{"title":"TGFbI和S100A4在肝细胞癌诊断价值及作用机制的初步分析。","authors":"Liang Zhao, Qinghua Shu, Bowen Sha, Miao Wu, Yufeng Zhang","doi":"10.5937/jomb0-54550","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The aim was to analyse the diagnostic value of transforming growth factor-beta-induced protein (TGF I) and S100 calcium-binding protein A4 (S100A4) on hepatocellular carcinoma (HCC) and to explore further the effects of TGF I and S100A4 on ferroptosis in HCC cells.</p><p><strong>Methods: </strong>We retrospectively analysed 76 patients with HCC admitted to our hospital from October 2022 to June 2023 and detected the differences in the expression of TGF I and S100A4 in cancerous tissues and paracancerous tissues to analyse their diagnostic and prognostic assessment value for HCC. Additionally, the HCC cell line HepG2 was purchased and transfected with TGF I and S100A4 abnormal expression plasmids to check changes in cell viability, oxidative stress damage, mitochondrial damage, and ferroptosis.</p><p><strong>Results: </strong>TGF I and S100A4 were upregulated in HCC tissues (P<0.05), and their combined detection exhibited excellent diagnostic effects for HCC. The levels of TGF I and S100A4 in patients who died prognostically were higher than those in surviving patients (P<0.05). An increase in the levels of TGF I and S100A4 indicates an elevated risk of prognostic death in patients. Upregulating TGF I and S100A4 expression in cell experiments activated HepG2 activity, inhibited apoptosis, mitochondrial and oxidative stress damage, and improved cell ferroptosis.</p><p><strong>Conclusions: </strong>TGF I and S100A4 are elevated in HCC and can potentially be clinical diagnostic indicators of HCC.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 3","pages":"400-411"},"PeriodicalIF":1.5000,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12357624/pdf/","citationCount":"0","resultStr":"{\"title\":\"Preliminary analysis of the diagnostic value and mechanism of action of TGFbI and S100A4 in hepatocellular carcinoma.\",\"authors\":\"Liang Zhao, Qinghua Shu, Bowen Sha, Miao Wu, Yufeng Zhang\",\"doi\":\"10.5937/jomb0-54550\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The aim was to analyse the diagnostic value of transforming growth factor-beta-induced protein (TGF I) and S100 calcium-binding protein A4 (S100A4) on hepatocellular carcinoma (HCC) and to explore further the effects of TGF I and S100A4 on ferroptosis in HCC cells.</p><p><strong>Methods: </strong>We retrospectively analysed 76 patients with HCC admitted to our hospital from October 2022 to June 2023 and detected the differences in the expression of TGF I and S100A4 in cancerous tissues and paracancerous tissues to analyse their diagnostic and prognostic assessment value for HCC. Additionally, the HCC cell line HepG2 was purchased and transfected with TGF I and S100A4 abnormal expression plasmids to check changes in cell viability, oxidative stress damage, mitochondrial damage, and ferroptosis.</p><p><strong>Results: </strong>TGF I and S100A4 were upregulated in HCC tissues (P<0.05), and their combined detection exhibited excellent diagnostic effects for HCC. The levels of TGF I and S100A4 in patients who died prognostically were higher than those in surviving patients (P<0.05). An increase in the levels of TGF I and S100A4 indicates an elevated risk of prognostic death in patients. Upregulating TGF I and S100A4 expression in cell experiments activated HepG2 activity, inhibited apoptosis, mitochondrial and oxidative stress damage, and improved cell ferroptosis.</p><p><strong>Conclusions: </strong>TGF I and S100A4 are elevated in HCC and can potentially be clinical diagnostic indicators of HCC.</p>\",\"PeriodicalId\":16175,\"journal\":{\"name\":\"Journal of Medical Biochemistry\",\"volume\":\"44 3\",\"pages\":\"400-411\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2025-06-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12357624/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Medical Biochemistry\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.5937/jomb0-54550\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Medical Biochemistry","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.5937/jomb0-54550","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Preliminary analysis of the diagnostic value and mechanism of action of TGFbI and S100A4 in hepatocellular carcinoma.
Background: The aim was to analyse the diagnostic value of transforming growth factor-beta-induced protein (TGF I) and S100 calcium-binding protein A4 (S100A4) on hepatocellular carcinoma (HCC) and to explore further the effects of TGF I and S100A4 on ferroptosis in HCC cells.
Methods: We retrospectively analysed 76 patients with HCC admitted to our hospital from October 2022 to June 2023 and detected the differences in the expression of TGF I and S100A4 in cancerous tissues and paracancerous tissues to analyse their diagnostic and prognostic assessment value for HCC. Additionally, the HCC cell line HepG2 was purchased and transfected with TGF I and S100A4 abnormal expression plasmids to check changes in cell viability, oxidative stress damage, mitochondrial damage, and ferroptosis.
Results: TGF I and S100A4 were upregulated in HCC tissues (P<0.05), and their combined detection exhibited excellent diagnostic effects for HCC. The levels of TGF I and S100A4 in patients who died prognostically were higher than those in surviving patients (P<0.05). An increase in the levels of TGF I and S100A4 indicates an elevated risk of prognostic death in patients. Upregulating TGF I and S100A4 expression in cell experiments activated HepG2 activity, inhibited apoptosis, mitochondrial and oxidative stress damage, and improved cell ferroptosis.
Conclusions: TGF I and S100A4 are elevated in HCC and can potentially be clinical diagnostic indicators of HCC.
期刊介绍：
The JOURNAL OF MEDICAL BIOCHEMISTRY (J MED BIOCHEM) is the official journal of the Society of Medical Biochemists of Serbia with international peer-review. Papers are independently reviewed by at least two reviewers selected by the Editors as Blind Peer Reviews. The Journal of Medical Biochemistry is published quarterly.
The Journal publishes original scientific and specialized articles on all aspects of
clinical and medical biochemistry,
molecular medicine,
clinical hematology and coagulation,
clinical immunology and autoimmunity,
clinical microbiology,
virology,
clinical genomics and molecular biology,
genetic epidemiology,
drug measurement,
evaluation of diagnostic markers,
new reagents and laboratory equipment,
reference materials and methods,
reference values,
laboratory organization,
automation,
quality control,
clinical metrology,
all related scientific disciplines where chemistry, biochemistry, molecular biology and immunochemistry deal with the study of normal and pathologic processes in human beings.
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