Journal of Medical Biochemistry最新文献

{"title":"Pramipexole enhances levodopa's therapeutic efficacy in Parkinson's disease: Role of glutaredoxin-1 (GRX1), peroxiredoxin3 (PRX3), thioredoxin (TRX), 8-hydroxy-2 -deoxyguanosine (8-OHDG), and neurosteroid dehydroepiandrosterone sulfate (DHEA-S).","authors":"Yu Shu, Jinjun Qian","doi":"10.5937/jomb0-59700","DOIUrl":"10.5937/jomb0-59700","url":null,"abstract":"<p><strong>Background: </strong>Parkinson's disease (PD) is characterised byprogressive neurodegeneration and dopamine deficiency.Levodopa remains a cornerstone treatment, but its longterm use is associated with motor complications andreduced efficacy. This study investigates the clinical benefits of combining pramipexole with Levodopa in PDpatients, focusing on improvements in oxidative stress,cognitive function, and motor control. Additionally, thestudy explores the role of novel biomarkers, includingglutaredoxin-1 (Grx1), peroxiredoxin-3 (Prx3), thioredoxin(Trx), 8-hydroxy-2'-deoxyguanosine (8-OHdG), and neurosteroid dehydroepiandrosterone sulfate (DHEA-S), inevaluating oxidative stress and neuroprotection.</p><p><strong>Methods: </strong>A total of 92 PD patients were enrolled andassigned to either a levodopa monotherapy group (n=46)or a pramipexole-levodopa combination group (n=46).Clinical efficacy was assessed using the Unified Parkinson'sDisease Rating Scale (UPDRS). Cognitive function wasevaluated using the Mini-Mental State Examination(MMSE) and the Montreal Cognitive Assessment (MoCA).Oxidative stress markers (SOD, GSH, GSH-PX, CAT, alongwith Grx1, Prx3, Trx, and 8-OHdG) were measured inserum samples. Additionally, DHEA-S was analysed as aneurosteroid biomarker to assess its potential role in cognitive and motor function improvements. Quality of life(QOL) was evaluated using the PDQ-39 questionnaire.</p><p><strong>Results: </strong>The combination therapy group exhibited a significantly higher effective rate (93.48% ) compared to the levodopa group (78.26% ) (P< 0.05). UPDRS scores were significantly lower in the combination group at 6- and12-week post-treatment (P < 0 .0 5 ). The combinationgroup also showed a significantly lower incidence ofadverse drug reactions (6.52% vs. 23.91% , P< 0.05). After3 months, the combination group displayed significantlyhigher levels of SOD, GSH, GSH-PX, CAT, Grx1, Prx3, andTrx, while 8-OHdG levels were significantly reduced, indicating enhanced neuroprotection and reduced oxidativestress. DHEA-S levels were also elevated, correlating withimproved MMSE and M oCA scores (P < 0 .0 5 ). Theobserved DHEA-S elevation in the combination group maybe due to pramipexole's dopaminergic modulation ofhypothalamic-pituitary-adrenal axis activity, potentiallyenhancing adrenal steroidogenesis. Alternatively, improvements in motor and cognitive function may reduce chronicstress, indirectly elevating DHEA-S. It suggests a neurosteroid-mediated cognitive benefit. QOL was significantlybetter in the combination group after 3 months of intervention (P< 0.05).</p><p><strong>Conclusions: </strong>Pramipexole combined with Levodopa significantly improves clinical outcomes, reduces adverse effects,enhances cognitive function, and alleviates oxidative stressin PD patients. The inclusion of novel biomarkers such asGrx1, Prx3, Trx, 8-OHdG, and DHEA-S provides deeperinsight into the molecular mechanisms underlying t","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"539-549"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596838/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical and clinical effects of continuous positive airway pressure combined with sildenafil on inflammatory and hemodynamic profiles in obese asthma patients with pulmonary arterial hypertension.","authors":"Wenmei Bai, Nana Wang, Zixuan Wang, Zhimin Wu, Xueying Luo, Fengsen Li","doi":"10.5937/jomb0-62326","DOIUrl":"10.5937/jomb0-62326","url":null,"abstract":"<p><strong>Background: </strong>Obesity-associated asthma complicated by pulmonary arterial hypertension (PAH) involves multifactorial biochemical dysregulation, including endothelial dysfunction, oxidative stress, and chronic inflammation. This study aimed to evaluate the combined biochemical and clinical effects of continuous positive airway pressure (CPAP) and sildenafil on pulmonary function, inflammatory biomarkers, and hemodynamic parameters in obese asthma patients with PAH.</p><p><strong>Methods: </strong>A total of 134 patients (BMI &gt;30 kg/m2, FEV1 &lt; 70% pred, PASP &gt; 30 mmHg) were enrolled and divided into a treatment group (n = 63, CPAP + sildenafil) and a control group (n = 71, sildenafil alone). Pulmonary and hemodynamic indices (PaÜ2, FiÜ2, PASP, OI, and PaO2/FiO2), lung function parameters (FEV1 , PEF), and asthma control test (ACT) scores were assessed at baseline and follow-up (6 and 12 months). Serum interleukin-6 (IL-6), tumor necrosis factor-a (TNF-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\">α</span>), and fractional exhaled nitric oxide (FeNO) were quantified to evaluate biochemical inflammation. Correlation analysis was performed between biomarker changes and ACT improvements.</p><p><strong>Results: </strong>The CPAP + sildenafil group showed significantly higher post-treatment PaÜ2 levels, lower PASP and FiÜ2 values, improved OI, and greater increases in PaO2/FiO2 and FEV1 percentages compared with the control group (P &lt; 0.001). Biochemical assays revealed substantial reductions in serum IL-6 and TNF-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\">α</span> levels, as well as FeNO concentrations, in the combination group. Post-treatment changes in IL-6 (r= 0.68, P &lt; 0.0001), TNF-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\">α</span> (r= 0.59, P &lt; 0.0001), and FeNO (r= 0.72, P &lt; 0.0001) were strongly correlated with improved ACT scores, suggesting a close biochemical-clinical relationship between airway inflammation and therapeutic response.</p><p><strong>Conclusions: </strong>CPAP combined with sildenafil exerts dual biochemical and clinical benefits in obese asthma patients with PAH by enhancing oxygenation, lowering pulmonary artery pressure, and suppressing inflammatory cytokine activity. The observed reductions in IL-6, TNF-<span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\">α</span>, and FeNO highlight the biochemical basis of treatment efficacy and the potential of these markers as laboratory indicators for monitoring therapeutic outcomes.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"626-635"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149024880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of serum inflammatory factors and hematologic parameters with lower limb deep vein thrombosis in neurocritical patients: A retrospective cohort study.","authors":"Yunqiu Zhu, Chun Wang, Jia Wang, Tian Shi, Weijie Tang, Huaping Zhou, Wanfei Guo, Hongbin Mei, Jixiang Bo","doi":"10.5937/jomb0-58954","DOIUrl":"10.5937/jomb0-58954","url":null,"abstract":"<p><strong>Background: </strong>The aim of this study was to investigate the mechanisms of postoperative deep vein thrombosis (DVT) of the lower extremities in neurocritical care patients, focusing on the interaction of inflammatory factors [D-dimer (DD), prothrombin time (PT), blood cell parameters (lymphocytes, platelets, plateletcrit) and coagulation function.</p><p><strong>Methods: </strong>A retrospective cohort study design was used to include 261 neurocritical care patients who underwent surgery between August 2019 and August 2021. They were categorized into DVT group (n = 121) and non-DVT group (n = 140) based on postoperative lower limb Doppler ultrasound findings. Preoperative coagulation indices (TT, DD), inflammatory markers [C reactive protein (CRP), procalcitonin (PCT), fibrinogen (FIB)], and blood cell parameters (hemoglobin, neutrophils, lymphocytes, platelet counts, and plateletcrit) were collected from the patients.</p><p><strong>Results: </strong>DD levels were significantly higher and TT shorter in the DVT group than in the non-DVT group (P&lt; 0.05); DD was positively correlated with PT-INR (P&lt; 0.001). In addition, lymphocyte and plateletcrit were significantly lower in the DVT group (P&lt; 0.05). Multifactorial analysis showed that age (OR = 1.027), days of hemostatic medication (OR = 1.203), postoperative infections (OR= 2.728), elevated DD (OR = 1.056), and shortened TT (OR = 0.840) were independent risk factors (P&lt; 0.05).</p><p><strong>Conclusions: </strong>The development of DVT in neurocritical patients is the result of a combination of hypercoagulation, inflammatory response, and immunosuppression.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"572-578"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596855/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149024959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk correlation analysis of serum adiponectin (APN), D-dimer (DD) and the neutrophil - lymphocyte ratio (NLR) with the prognosis of diabetic foot ulcer infection.","authors":"Guodong Guo, Xiaohui Zhang, Peng Fang, Kangkanng Li, Chaoyang Wang, Gang Liu, Huijuan Ma","doi":"10.5937/jomb0-60169","DOIUrl":"10.5937/jomb0-60169","url":null,"abstract":"<p><strong>Background: </strong>To investigate the connections between diabetic foot ulcer infection patient prognosis and disease severity, we measured serum adiponectin (APN) and D-dimer (D-D) levels and the neutrophil lymphocyte ratio (NLR).</p><p><strong>Methods: </strong>A total of 292 patients with diabetic foot ulcer infection admitted to our hospital from February 2024 to February 2025 were selected and divided into mild (n = 95), moderate (n = 139) and severe (n = 58) patients according to the severity of the disease. The levels of serum APN and D-D and the NLR in patients with different severity levels were compared. To analyse the serum APN, D-D, and NLR, patients were followed up for one year, and the prognoses of the patients were statistically analysed.</p><p><strong>Results: </strong>Comparisons of the serum APN, D-D and NLR levels among patients with different disease severities revealed that the serum APN levels in patients with moderate disease were lower than those in patients with mild disease (P&lt;0.05). The serum APN of severe patients was 5.35± 0.98, and that of moderate patients was 7.64±1.25, which was lower than that of mild patients (9.19±1.73; P&lt;0.05), and the serum APN level of severe patients was lower than that of moderate patients (P&lt;0.05). Comparisons of the serum D-D and NLR levels revealed that the serum D-D and NLR in severe patients were 3.49±0.72 and 2.86±0.58, respectively, and those in moderate patients were 3.02±0.63 and 2.24±0.46, respectively, both of which were greater than those in mild patients, which were 2.43±0.51 and 1.71±0.33 (P&lt;0.05). The levels of serum D-D and the NLR were greater in severe patients than in moderate patients (P&lt;0.05). Spearman correlation analysis revealed that the severity of disease in diabetic foot ulcer infection patients was positively related to the serum D-D level and NLR (r=0.387, P&lt;0.001; r=0.461, P&lt;0.001) and negatively related to the serum APN level (r=-0.414, P&lt;0.001). ROC analysis revealed that the optimal cutoff values of the serum APN, D-D and NLR for predicting poor prognosis in patients were 5.73 mg/L, 3.06 mg/L and 2.12, respectively; the sensitivities were 78.57%, 82.14% and 85.71%, respectively; and the specificities were 76.56%, 67.19% and 73.44%, respectively. The areas under the curve (AUCs) were 0.793, 0.784, and 0.818, respectively. The specificity and AUC of the combination of the three methods were 98.44% and 0.918, respectively.</p><p><strong>Conclusions: </strong>Serum APN and D-D levels and the NLR in patients with diabetic foot ulcer infection are related to disease severity. The serum APN, D-D and NLR can be used as sensitive indicators for predicting poor patient prognosis.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"558-565"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic value and risk correlation of serum heart-type fatty acid-binding protein (H-FABP) and osteoprotegerin (OPG) in chronic heart failure.","authors":"Zheng Weifeng, Liu Mingda, Xu Sanduo, Xu Wang","doi":"10.5937/jomb0-60310","DOIUrl":"10.5937/jomb0-60310","url":null,"abstract":"<p><strong>Background: </strong>To analyse the value of H-FABP and OPG in determining the severity of heart function in those suffering from long-term heart failure.</p><p><strong>Methods: </strong>A total of 196 patients with persistent heart failure who were admitted to our hospital between June 2023 and October 2024 were chosen. A comparison of the three groups of chronic heart failure patients' general clinical information and H-FABP and OPG levels was performed. The associations between OPG and H-FABP and various clinical forms of chronic heart failure, heart failure severity, and measurement markers related to echocardiography were examined. To assess the diagnostic utility of NT-proBNP, H-FABP, and OPG detection alone and in combination for HFrEF and HFpEF patients. All patients were monitored for three to six months following their discharge.</p><p><strong>Results: </strong>There was a positive correlation (r=0.61) between H-FABP and the NYHA classification, LA (r=0.46), LV (r=0.51), HS-TnT (r=0.31), NT-proBNP (r=0.58), SUA (r=0.38), etc., and negatively correlated with the LVEF (r=-0.76), NT-proBNP (r=0.49), etc., and negatively correlated with the LVEF (r=-0.60) (P&lt;0.05). Binary logistic regression analysis of NT-proBNP, H-FABP, and OPG levels and endpoint events revealed that NT-proBNP had a high value in predicting endpoint events. The readmission rate and mortality rate of patients with chronic heart failure increased with increasing NT-proBNP concentration. Serum H-FABP and OPG all have high diagnostic value for HFrEF. Compared with the traditional biomarker NT-proBNP, H-FABP had greater sensitivity (94.9%) and specificity (83.1%) in the diagnosis of HFrEF, whereas OPG had greater sensitivity (92.3%) and lower specificity (57.7%). H-FABP and OPG can significantly improve the sensitivity (86.44%) and specificity (89.74%) in the diagnosis of patients with HFrEF. Serum NT-proBNP, H-FABP and OPG all have high diagnostic value for HFpEF. Compared with the traditional biomarker NT-proBNP, H-FABP has greater sensitivity (91.7%) and specificity (82.0%) in the diagnosis of HFpEF, whereas OPG has greater specificity (82.0%) and lower sensitivity (58.3%).</p><p><strong>Conclusions: </strong>The combined detection of H-FABP, OPG, and NT-proBNP can be used as an essential strategy for the early detection of decreased cardiac function in heart failure patients.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"690-698"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596845/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149024921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of platelet-rich fibrin combined with hydroxychloroquine on the inflammatory response; bone metabolism; and cell differentiation ability in temporomandibular joint osteoarthritis.","authors":"Yuhan Zhao, Hongwei Dai","doi":"10.5937/jomb0-60052","DOIUrl":"10.5937/jomb0-60052","url":null,"abstract":"<p><strong>Background: </strong>This study aims to explore the regulatory effects of platelet-rich fibrin (PRF) on serum inflammatory response, bone metabolic balance and cell differentiation ability in patients with temporomandibular joint osteoarthritis (TMJ-OA) at the molecular level.</p><p><strong>Methods: </strong>Sixty patients were randomly divided into Group A and Group B (30 cases in each group). Group A was treated with meloxicam dispersible combined with hydrox-ychloroquine sulfate (HCQ); Group B was supplemented with autologous PRF intra-articular injection on the basis of Group A. Fasting venous blood was collected before treat-ment and 3 months after treatment. Inflammatory and bone metabolism markers such as IL-ip, IL-6, TNF-a, OPG, OPN, ALP and Runx2 were detected by e L iSA, and coagulation indicators such as A PTT and PT were deter-mined by an automatic coagulation analyzer.</p><p><strong>Results: </strong>After treatment, the inflammatory factors (IL-ip, IL-6, TNF-a) in both groups decreased significantly, but the decrease in group B was more significant (P&lt; 0.05). Bone metabolism indicators showed that the improvement in group B was significantly better than that in group A (P&lt; 0.05). Among the cell differentiation-related factors, the expression levels of Runx2, TGF-pi and Sox9 in group B were significantly increased compared with those before treatment (P&lt; 0.05), and were higher than those in group A (P&lt; 0.05).</p><p><strong>Conclusions: </strong>The use of PRF can optimize the therapeutic effect of TMJ-OA, improve inflammatory responses, promote cell differentiation and improve coagulation function parameters.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"586-593"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596861/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardiac function, heart failure indicators and inflammatory factors in continuous mild hypothermic hemodialysis patients after valvular heart disease surgery.","authors":"Mao Yun, Zhu Li, Chen Huilan, Li Danxin, Yuan Chikai, Wang Renqiang, Liu Jian","doi":"10.5937/jomb0-60316","DOIUrl":"10.5937/jomb0-60316","url":null,"abstract":"<p><strong>Background: </strong>To compare the effects of continuous hemodialysis at normal temperature and mild hypothermia in the treatment of cardiogenic shock after valvular heart disease surgery and its influences on patients' cardiac function, heart failure indicators and inflammatory factors.</p><p><strong>Methods: </strong>After surgery for valvular heart disease, 180 patients with cardiogenic shock were brought to our hospital between January 2023 and December 2024. Patients in the control group received continuous hemodialysis treatment at normal temperature, whereas patients in the observation group received continuous hemodialysis treatment at mild hypothermia. The postoperative drainage volume, blood purification time, ventilator assistance time, ICU stay time, and occurrence of death, arrhythmia and infection were compared between the two groups. Cardiac function indicators, laboratory indicators of heart failure, and serum creatinine (Cr) and inflammatory factor data were compared between the two patient groups before and after therapy.</p><p><strong>Results: </strong>Postoperative drainage volume, ventilator assistance time, intensive care unit (ICU) stay, blood purification time and mortality rate (P&lt;0.05) were analysed. The laboratory measurements of BNP , hs-CRP , and CR-related heart failure did not differ before treatment (P&gt;0.05). BNP , hs-CRP , and CR-related heart failure were laboratory markers that were reduced in both groups after treatment; the levels in the observation group were lower than those in the control group (P&lt;0.05). Tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1 (IL-1) were present at the same pretreatment levels in both groups (P&gt;0.05). The levels decreased after treatment and were lower in the observation group than in the control group (P&lt;0.05).</p><p><strong>Conclusions: </strong>For patients with cardiogenic shock after valvular heart disease surgery, mild hypothermia hemodialysis treatment can further reduce the postoperative drainage volume and hemopurification time, promote early recovery, and reduce the mortality rate. Moreover, mild hypothermia hemodialysis treatment can further improve the cardiac function of patients and alleviate heart failure. The levels of inflammatory factors in the patient's body should be reduced.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"699-706"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149024922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variations and implications of Irisin, FGF-23, and N-MID osteocalcin in diabetic osteoporosis patients undergoing acarbose plus sitagliptin therapy: A prospective cohort analysis.","authors":"Lilan Zhou, Linghua Peng","doi":"10.5937/jomb0-60451","DOIUrl":"10.5937/jomb0-60451","url":null,"abstract":"<p><strong>Background: </strong>The present study intends to explore the dynamic variations in Irisin, fibroblast growth factor 23 (FGF-23), and N-terminal propeptide of osteocalcin (N-MID) among diabetes mellitus osteoporosis (DOP) patients undergoing treatment with acarbose plus sitagliptin, as well as their ability to predict bone metabolic status and fracture risk. The ultimate objective is to identify potential biomarkers for optimizing DOP therapy.</p><p><strong>Methods: </strong>From January 2023 to March 2024, 124 DOP patients admitted to our hospital were administered acarbose in combination with sitagliptin. For comparison, 119 uncomplicated diabetes mellitus (DM) patients were recruited as controls. Serum levels of Irisin, FGF-23, and N-MID were tested both at baseline and post-treatment. Data on bone mineral density (BMD), bone metabolic markers, and fracture occurrences during follow-up were also collected.</p><p><strong>Results: </strong>Compared with uncomplicated DM individuals, DOP patients exhibited a rise in FGF-23 (P&lt; 0.05) and a decrease in Irisin and N-MID (P&lt; 0.05). Irisin + FGF-23 + N-MID combination demonstrated 87.10% sensitivity and 74.58% specificity in diagnosing DOP (AU C= 0.867). Treatment with acarbose and sitagliptin contributed to reduced FGF-23 in DOP patients, along with increased Irisin and N-MID (P&lt; 0.05). BMD was positively influenced by irisin but negatively affected by FGF-23. Fracture prediction efficacy was markedly enhanced (AU C= 0.822) by combining the three indices, with statistical superiority over single-index analysis (P&lt; 0.05).</p><p><strong>Conclusions: </strong>The combined detection of Irisin, FGF-23, and N-MID enables early detection of DOP while allowing for dynamic assessment of treatment outcomes and fracture risk.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"529-538"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149024989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic value of the HALP index, erythrocyte sedimentation rate, and rheumatoid factor in rheumatoid arthritis: A laboratory-based multivariate analysis.","authors":"Xiaohua Xu, Xiaohua Huang, Lu Yongfang, Zeng Defei","doi":"10.5937/jomb0-61610","DOIUrl":"10.5937/jomb0-61610","url":null,"abstract":"<p><strong>Background: </strong>Biochemical and hematological indices play a critical role in evaluating systemic inflammation and disease activity in rheumatoid arthritis (RA). This study investigated the prognostic value of the hemoglobin-albumin-lymphocyte-platelet (HALP) index, in combination with erythrocyte sedimentation rate (ESR) and rheumatoid factor (RF), for predicting RA disease progression.</p><p><strong>Methods: </strong>A retrospective analysis was performed on 146 RA patients admitted between December 2022 and December 2023. Laboratory variables included HALP index, ESR, RF, C-reactive protein, anti-CCP antibodies, hemoglobin, albumin, lymphocyte and platelet counts, and serum creatinine. Measurements were obtained using standardized automated hematology and biochemical analyzers. Disease activity and radiographic joint damage scores were also assessed. Statistical approaches included correlation analysis, regression analysis, and receiver operating characteristic (ROC) curve evaluation.</p><p><strong>Results: </strong>The HALP index, ESR, and RF were significantly associated with RA disease progression. The HALP index demonstrated a strong correlation with disease activity (r=0.602, P&lt;0.001). ROC analysis showed an area under the curve (AUC) of 0.858 for HALP, 0.841 for ESR, and 0.924 for RF. When combined in a multivariate regression model, the predictive performance improved (AUC=0.943).</p><p><strong>Conclusions: </strong>The HALP index, as a composite biochemical marker, in combination with ESR and RF provides significant prognostic information for RA progression. These findings underscore the importance of laboratory-based indices in refining prognostic assessments and highlight their potential role in personalized disease management.</p>","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"645-654"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596860/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149024992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The application value of serum Th1/Th2 cytokines combined with tumour markers in the diagnosis of HR-HPV-positive cervical cancer.","authors":"Xia Cao, Xuemei Zhang, Jingpo Zhang, Xiaodang Zhong, Shanshan Hu","doi":"10.5937/jomb0-59352","DOIUrl":"10.5937/jomb0-59352","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;To determine the efficacy of tumour markers in conjunction with serum helper T-cell 1 (Th1) and helper T-cell 2 (Th2) cytokines for the detection of HR-HPV-positive cervical cancer.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;49 patients with HR-HPV-positive benign cervical diseases of the same age group who were admitted to the hospital between January 2020 and June 2024 were chosen as the control group, and 49 patients with HR-HPV-positive cervical cancer were chosen as the study group. Serum Th1/Th2 cytokine levels of patients with various clinicopathological characteristics in the study group were compared with the basic data, tumour markers, and serum Th1/Th2 cytokines [interleukin (IL)-10, IL-6, interferon-g (IFN-g), and tumour necrosis factor-a (TNF-&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;α&lt;/span&gt;)] and tumour markers between the two groups. The association between tumour markers and serum Th1/Th2 cytokine levels in patients with HR-HPV-positive cervical cancer was examined using Pearson correlation analysis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;International Federation of Obstetrics and Gynecology (FIGO) stages III-IV patients in the study group had greater serum levels of IL-10, IL-6, IFN-g, and TNF-&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;α&lt;/span&gt; than the control group (P&lt; 0.05), and the levels of these substances in the patients with a myometrial invasion depth 1/2 were all higher than in the patients with a myometrial invasion depth &lt;1/2 (P&lt; 0.05). SCC-Ag, CA199, and there was a significant correlation (P&lt; 0.05) between CYFRA21-1 levels and blood levels of IL-10, IL-6, IFN-g, and TNF-&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;α&lt;/span&gt; in patients with HR-HPV-positive cervical cancer. The AUCs of IL-10, IL-6, IFN-g and TNF-&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;α&lt;/span&gt; for the single diagnosis of HR-HPV-positive cervical cancer were 0.809, 0.773, 0.801 and 0.794, respectively, and the AUCs of SCC-Ag, CA199 and CYFRA21-1 for the single diagnosis of HR-HPV-positive cervical cancer were 0.831 and 0, respectively. 728, 0.789. The AUC of the combined diagnosis of HR-HPV-positive cervical cancer by serum IL-10, IL-6, IFN-g, TNF-&lt;span style=\"color: rgb(32, 33, 34); font-family: sans-serif; font-size: 16px; background-color: rgb(248, 249, 250);\"&gt;α&lt;/span&gt;, SCC-Ag, CA199, and CYFRA21-1 was 0.927. The AUC of the combined diagnosis was greater than that of the individual diagnosis of each index (Z = 2 .1 16, 2.690, 2.341, 2.565, 1.957, 3.351, 2.631, P= 0.034, 0.007, 0.019, 0.010, 0.039, 0.001, 0.009).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusions: &lt;/strong&gt;The combined detection of serum Th1/Th2 cytokine imbalance (elevated IL-6 and IL","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"45 3","pages":"579-585"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13596854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149025012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}