Differently expressed miRNA in plasma samples of immune thrombocytopenic purpura patients and its clinical significance.

Background: To guide clinical doctors, current work has been designed to explore the abnormal expressions of miRNAs in plasma samples of patients with immune thrombocytopenic purpura (ITP).

Methods: Bioinformatic analysis was performed using the GSE80401 chip. The study subjects were recruited from the First People's Hospital of Lianyungang between May 2021 and December 2023. 48 ITP patients admitted to the intensive care unit were enrolled. miRNA levels were examined using real-time polymerase chain reaction. All data were analysed using SPSS 22.0 software. The potential diagnosis value of the significantly up-regulated and down-regulated miRNAs was evaluated using a receiver operator characteristic (ROC) curve.

Results: We performed bioinformatical analysis on the GSE80401 chip and identified the differently expressed miRNAs in ITP patients compared to the controls, and the results of the heat map showed the results. GO and pathway analysis revealed the process that involved the differently expressed miRNAs. Next, the results of RT-qPCR analysis showed the levels of miR-877-3p, miR-425-3p, miR-122-5p, miR-1281, and miR-1825 were significantly increased. In contrast, the miR-3945, miR-4430, miR-3158-5p, miR-3131, and miR-4655-3p levels were markedly decreased in plasma samples of ITP patients compared with the controls. Finally, results showed that the area under the ROC curve of miRNAs was as follows: miR-877-3p, 0.9349, miR-425-3p, 0.8607, miR-1281, 0.7131, miR-1825, 0.8928, miR-3945, 0.8459, miR-4430, 0.8112, miR-3158-5p, 0.6059, miR-3131, 0.8989, suggesting that the above miRNAs may serve as biomarkers for distinguishing the ITP patients from healthy controls.

Conclusions: miRNAs may have predictive value for the diagnosis of ITP. The results of current work may provide new clues for the pathogenesis of ITP, which in turn offers a new theoretical basis and therapeutic tools for the clinical diagnosis and treatment of ITP.