Differently expressed miRNA in plasma samples of immune thrombocytopenic purpura patients and its clinical significance.

IF 1.5 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Medical Biochemistry Pub Date : 2025-08-21 DOI:10.5937/jomb0-52498

Yuqian Yao, Hemeng Zhao, Xiaoyu Zhu, Yafei Fang, Yue Feng

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引用次数: 0

Abstract

Background: To guide clinical doctors, current work has been designed to explore the abnormal expressions of miRNAs in plasma samples of patients with immune thrombocytopenic purpura (ITP).

Methods: Bioinformatic analysis was performed using the GSE80401 chip. The study subjects were recruited from the First People's Hospital of Lianyungang between May 2021 and December 2023. 48 ITP patients admitted to the intensive care unit were enrolled. miRNA levels were examined using real-time polymerase chain reaction. All data were analysed using SPSS 22.0 software. The potential diagnosis value of the significantly up-regulated and down-regulated miRNAs was evaluated using a receiver operator characteristic (ROC) curve.

Results: We performed bioinformatical analysis on the GSE80401 chip and identified the differently expressed miRNAs in ITP patients compared to the controls, and the results of the heat map showed the results. GO and pathway analysis revealed the process that involved the differently expressed miRNAs. Next, the results of RT-qPCR analysis showed the levels of miR-877-3p, miR-425-3p, miR-122-5p, miR-1281, and miR-1825 were significantly increased. In contrast, the miR-3945, miR-4430, miR-3158-5p, miR-3131, and miR-4655-3p levels were markedly decreased in plasma samples of ITP patients compared with the controls. Finally, results showed that the area under the ROC curve of miRNAs was as follows: miR-877-3p, 0.9349, miR-425-3p, 0.8607, miR-1281, 0.7131, miR-1825, 0.8928, miR-3945, 0.8459, miR-4430, 0.8112, miR-3158-5p, 0.6059, miR-3131, 0.8989, suggesting that the above miRNAs may serve as biomarkers for distinguishing the ITP patients from healthy controls.

Conclusions: miRNAs may have predictive value for the diagnosis of ITP. The results of current work may provide new clues for the pathogenesis of ITP, which in turn offers a new theoretical basis and therapeutic tools for the clinical diagnosis and treatment of ITP.

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免疫性血小板减少性紫癜患者血浆样品中miRNA表达差异及其临床意义。

背景：探讨免疫性血小板减少性紫癜（ITP）患者血浆样本中mirna的异常表达，以指导临床医生。方法：采用GSE80401芯片进行生物信息学分析。研究对象于2021年5月至2023年12月在连云港市第一人民医院招募。48例ITP患者入组重症监护室。实时聚合酶链反应检测miRNA水平。所有数据采用SPSS 22.0软件进行分析。使用受试者操作特征（ROC）曲线评估显著上调和下调的mirna的潜在诊断价值。结果：我们对GSE80401芯片进行了生物信息学分析，鉴定出ITP患者与对照组相比表达差异的mirna，热图结果显示了结果。氧化石墨烯和通路分析揭示了涉及不同表达的mirna的过程。接下来，RT-qPCR分析结果显示miR-877-3p、miR-425-3p、miR-122-5p、miR-1281、miR-1825水平显著升高。相比之下，与对照组相比，ITP患者血浆样本中的miR-3945、miR-4430、miR-3158-5p、miR-3131和miR-4655-3p水平明显降低。最后，结果显示，mirna的ROC曲线下面积为：miR-877-3p， 0.9349, miR-425-3p, 0.8607, miR-1281, 0.7131, miR-1825, 0.8928, miR-3945, 0.8459, miR-4430, 0.8112, miR-3158-5p, 0.6059, miR-3131, 0.8989，提示上述mirna可能作为区分ITP患者与健康对照的生物标志物。结论：mirna可能对ITP的诊断具有预测价值。本研究结果可能为ITP的发病机制提供新的线索，从而为ITP的临床诊断和治疗提供新的理论依据和治疗工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Medical Biochemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

3.00

自引率

12.00%

发文量

审稿时长

>12 weeks

期刊介绍： The JOURNAL OF MEDICAL BIOCHEMISTRY (J MED BIOCHEM) is the official journal of the Society of Medical Biochemists of Serbia with international peer-review. Papers are independently reviewed by at least two reviewers selected by the Editors as Blind Peer Reviews. The Journal of Medical Biochemistry is published quarterly. The Journal publishes original scientific and specialized articles on all aspects of clinical and medical biochemistry, molecular medicine, clinical hematology and coagulation, clinical immunology and autoimmunity, clinical microbiology, virology, clinical genomics and molecular biology, genetic epidemiology, drug measurement, evaluation of diagnostic markers, new reagents and laboratory equipment, reference materials and methods, reference values, laboratory organization, automation, quality control, clinical metrology, all related scientific disciplines where chemistry, biochemistry, molecular biology and immunochemistry deal with the study of normal and pathologic processes in human beings.