膝关节骨性关节炎患者外周血液CXCL12和S100A12水平及其与严重程度的相关性

IF 2 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Medical Biochemistry Pub Date : 2025-01-24 DOI:10.5937/jomb0-46310

LijiangTao, He Minjuan, Lu Yisheng, Zheng Jie, Ye Yili

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引用次数: 0

摘要

背景：本文旨在研究CXCL12和S100A12在膝关节骨性关节炎（OS）患者外周血（PB）和滑液（SF）中的表达，并分析其与膝关节骨性关节炎（OS）严重程度的相关性。方法：选择2020年1月至2022年12月在我院治疗的60例膝关节OS患者作为实验组，选择60例年龄相近的健康膝关节作为对照组。120例受试者清晨空腹静脉血抽取，关节手术或注射透明质酸钠时抽取SF。将收集的PB和SF放入-80°C的冰箱中。采用酶联免疫吸附法（Elisa）检测PB和SF组织中CXCL12和S100A12的水平。结果：CXCL12和S100A12在PB和SF中的表达水平与Kmurl分级和WOMAC评分存在相关性。观察组PB、SF中CXCL12、S100A12水平均高于对照组。实验组PB和SF中CXCL12和S100A12水平差异有统计学意义。膝关节OS Kmurl分级越高，PB和SF中CXCL12和S100A12的浓度越高。膝关节OS患者PB中CXCL12、S100A12水平与WOMAC评分呈正相关（r分别为0.767、0.521）。结论：PB、SF中CXCL12、S100A12水平可为膝关节OS的评价及预后提供依据。

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Serum CXCL12 and S100A12 levels in peripheral blood fluid and their correlation with severity in patients with knee osteoarthritis.

Background: This paper aimed to investigate the expression of CXCL12 and S100A12 in peripheral blood (PB) and synovial fluid (SF) of patients with knee osteoarthritis (OS) and to analyze the correlation between them and the severity of knee OS.

Methods: Sixty patients with knee OS treated in our hospital from January 2020 to December 2022 were selected as the experimental group, and 60 healthy knee joints with similar ages were selected as the control group. The fasting venous blood of 120 subjects was drawn in the early morning, and the SF was extracted during joint operation or sodium hyaluronate injection. Put the collected PB and SF in the refrigerator at -80 °C. The levels of CXCL12 and S100A12 in PB and SF were detected by enzyme-linked immunosorbent assay (Elisa).

Results: The correlation between the levels of CXCL12 and S100A12 in PB and SF and Kmurl L grade and WOMAC score. The levels of CXCL12 and S100A12 in PB and SF in the observation group were higher than those in the control group. There were significant differences in the levels of CXCL12 and S100A12 in PB and SF in the experimental group. The higher the Kmurl grade of knee OS, the higher the concentration of CXCL12 and S100A12 in PB and SF. The levels of CXCL12 and S100A12 in PB of knee OS were positively correlated with WOMAC score (r=0.767, 0.521, respectively, P<0.05); see Figure 1. The levels of CXCL12 and S100A12 in SF of knee OS were positively correlated with WOMAC score (r=0.663, 0.357 respectively, P<0.05). The levels of CXCL12 and S100A12 in PB and SF are positively correlated with the severity of knee OS.

Conclusions: The levels of CXCL12 and S100A12 in PB and SF can provide the basis for the evaluation and prognosis of knee OS.

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来源期刊

Journal of Medical Biochemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

3.00

自引率

12.00%

发文量

审稿时长

>12 weeks

期刊介绍： The JOURNAL OF MEDICAL BIOCHEMISTRY (J MED BIOCHEM) is the official journal of the Society of Medical Biochemists of Serbia with international peer-review. Papers are independently reviewed by at least two reviewers selected by the Editors as Blind Peer Reviews. The Journal of Medical Biochemistry is published quarterly. The Journal publishes original scientific and specialized articles on all aspects of clinical and medical biochemistry, molecular medicine, clinical hematology and coagulation, clinical immunology and autoimmunity, clinical microbiology, virology, clinical genomics and molecular biology, genetic epidemiology, drug measurement, evaluation of diagnostic markers, new reagents and laboratory equipment, reference materials and methods, reference values, laboratory organization, automation, quality control, clinical metrology, all related scientific disciplines where chemistry, biochemistry, molecular biology and immunochemistry deal with the study of normal and pathologic processes in human beings.