Apoptosis in prostate carcinoma tissue: The role of caspase-3, caspase-1, and alkaline DNase activity.

IF 1.5 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Medical Biochemistry Pub Date : 2025-09-05 DOI:10.5937/jomb0-57574

Andrej Veljkovic, Jovan Hadzi-Djokic, Gordana Kocic, Xiaobo Li, Stefanos Roumeliotis, Dušan Sokolović, Aleksandra Klisic

{"title":"Apoptosis in prostate carcinoma tissue: The role of caspase-3, caspase-1, and alkaline DNase activity.","authors":"Andrej Veljkovic, Jovan Hadzi-Djokic, Gordana Kocic, Xiaobo Li, Stefanos Roumeliotis, Dušan Sokolović, Aleksandra Klisic","doi":"10.5937/jomb0-57574","DOIUrl":null,"url":null,"abstract":"Background: Prostate glandular tissue maintains a delicate balance between cellular proliferation and programmed cell death (apoptosis), ensuring the preservation of normal glandular architecture in healthy individuals. Disruption of this equilibrium - whether due to excessive proliferation or impaired apoptotic mechanisms - can contribute to the initiation and progression of prostate cancer. The objective of this study was to evaluate the expression and activity of caspase-3, caspase-1, and alkaline deoxyribonuclease (DNase) in prostate cancer tissue and tumour-adjacent tissue in comparison to clinically healthy prostate tissue. The aim was to determine whether alterations in these parameters could serve as early biomarkers for the transformation of surrounding tissue into a precancerous phenotype.Methods: The concentration of caspase-3 and caspase-1, as well as the activity of alkaline DNase, were examined in prostate tissue samples, including cancerous tissue, adjacent tissue near the tumour, and surrounding healthy tissue.Results: The results revealed a significant reduction in caspase-3 levels in cancerous tissue (p<0.05), with an even more pronounced decrease in the adjacent peritumoural tissue (p<0.05). In contrast, caspase-1 levels were markedly elevated in both cancerous tissue (p<0.00001) and the surrounding non-malignant peritumoural tissue (p<0.0005). Similarly, alkaline DNase activity (both total and specific) was significantly increased in cancerous tissue (p<0.00001), with a moderate but statistically significant elevation in the tumour-adjacent tissue (p<0.000017) compared to control tissue.Conclusions: These findings suggest a disruption in the interplay between caspase-3 and alkaline DNase, potentially as a consequence of necrotic processes or enzyme release from inhibitory complexes. Furthermore, the increased expression of caspase-1 implies that inflammatory responses may play a role in tumourigenesis.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"44 6","pages":"1297-1304"},"PeriodicalIF":1.5000,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12497462/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Medical Biochemistry","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.5937/jomb0-57574","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Prostate glandular tissue maintains a delicate balance between cellular proliferation and programmed cell death (apoptosis), ensuring the preservation of normal glandular architecture in healthy individuals. Disruption of this equilibrium - whether due to excessive proliferation or impaired apoptotic mechanisms - can contribute to the initiation and progression of prostate cancer. The objective of this study was to evaluate the expression and activity of caspase-3, caspase-1, and alkaline deoxyribonuclease (DNase) in prostate cancer tissue and tumour-adjacent tissue in comparison to clinically healthy prostate tissue. The aim was to determine whether alterations in these parameters could serve as early biomarkers for the transformation of surrounding tissue into a precancerous phenotype.

Methods: The concentration of caspase-3 and caspase-1, as well as the activity of alkaline DNase, were examined in prostate tissue samples, including cancerous tissue, adjacent tissue near the tumour, and surrounding healthy tissue.

Results: The results revealed a significant reduction in caspase-3 levels in cancerous tissue (p<0.05), with an even more pronounced decrease in the adjacent peritumoural tissue (p<0.05). In contrast, caspase-1 levels were markedly elevated in both cancerous tissue (p<0.00001) and the surrounding non-malignant peritumoural tissue (p<0.0005). Similarly, alkaline DNase activity (both total and specific) was significantly increased in cancerous tissue (p<0.00001), with a moderate but statistically significant elevation in the tumour-adjacent tissue (p<0.000017) compared to control tissue.

Conclusions: These findings suggest a disruption in the interplay between caspase-3 and alkaline DNase, potentially as a consequence of necrotic processes or enzyme release from inhibitory complexes. Furthermore, the increased expression of caspase-1 implies that inflammatory responses may play a role in tumourigenesis.

Abstract Image

查看原文本刊更多论文

前列腺癌组织凋亡：caspase-3、caspase-1和碱性dna酶活性的作用。

背景：前列腺组织在细胞增殖和程序性细胞死亡（凋亡）之间保持着微妙的平衡，确保了健康个体正常腺体结构的保存。这种平衡的破坏——无论是由于过度增殖还是凋亡机制受损——都可能导致前列腺癌的发生和发展。本研究的目的是评估caspase-3、caspase-1和碱性脱氧核糖核酸酶（DNase）在前列腺癌组织和肿瘤邻近组织中的表达和活性，并与临床健康前列腺组织进行比较。目的是确定这些参数的改变是否可以作为周围组织转化为癌前表型的早期生物标志物。方法：检测前列腺癌组织、癌旁组织及周围健康组织中caspase-3、caspase-1的浓度及碱性dna酶活性。结果：结果显示癌组织中caspase-3水平显著降低(结论：这些发现表明caspase-3和碱性dna酶之间的相互作用被破坏，可能是坏死过程或抑制复合物释放酶的结果。此外，caspase-1表达的增加表明炎症反应可能在肿瘤发生中起作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Medical Biochemistry BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

3.00

自引率

12.00%

发文量

审稿时长

>12 weeks

期刊介绍： The JOURNAL OF MEDICAL BIOCHEMISTRY (J MED BIOCHEM) is the official journal of the Society of Medical Biochemists of Serbia with international peer-review. Papers are independently reviewed by at least two reviewers selected by the Editors as Blind Peer Reviews. The Journal of Medical Biochemistry is published quarterly. The Journal publishes original scientific and specialized articles on all aspects of clinical and medical biochemistry, molecular medicine, clinical hematology and coagulation, clinical immunology and autoimmunity, clinical microbiology, virology, clinical genomics and molecular biology, genetic epidemiology, drug measurement, evaluation of diagnostic markers, new reagents and laboratory equipment, reference materials and methods, reference values, laboratory organization, automation, quality control, clinical metrology, all related scientific disciplines where chemistry, biochemistry, molecular biology and immunochemistry deal with the study of normal and pathologic processes in human beings.