Journal of Functional Biomaterials最新文献

{"title":"Developing Bioengineered 3D-Printed Composite Scaffolds with Antimicrobial Potential for Bone Tissue Regeneration.","authors":"Andreea Trifan, Eduard Liciu, Cristina Busuioc, Izabela-Cristina Stancu, Adela Banciu, Carmen Nicolae, Mihai Dragomir, Doru-Daniel Cristea, Rosina-Elena Sabău, David-Andrei Nițulescu, Alexandru Paraschiv","doi":"10.3390/jfb16060227","DOIUrl":"10.3390/jfb16060227","url":null,"abstract":"<p><p>This research activity proposes to produce composite hydrogel-bioactive glass. The primary purpose of this research is to develop and optimize 3D-printed scaffolds using doped bioglass, aimed at enhancing bone regeneration in bone defects. The bioglass, a bioactive material known for its bone-bonding ability (SiO₂-P₂O₅-CaO-Na₂O), co-doped with europium and silver was synthesized and doped to improve its biological properties. This doped bioglass was then combined with a biocompatible hydrogel, chosen for its adequate cellular response and printability. The composite material was printed to form a scaffold, providing a structure that not only supports the damaged bone but also encourages osteogenesis. A variety of methods were employed to assess the rheological, compositional, and morphological characteristics of the samples: Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) coupled with energy-dispersive X-ray spectroscopy (EDS). Additionally, simulated body fluid (SBF) immersion for bioactivity monitoring and immunocytochemistry for cell viability were used to evaluate the biological response of the scaffolds.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 6","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194729/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What Are the Best Biocompatible Materials for Extracorporeal Membrane Oxygenation.","authors":"Junya Hagiwara, Jeffrey D DellaVolpe, Yuichi Matsuzaki","doi":"10.3390/jfb16060226","DOIUrl":"10.3390/jfb16060226","url":null,"abstract":"<p><p>Extracorporeal membrane oxygenation (ECMO) is a crucial life support therapy for patients with severe cardiac and respiratory failure. However, the complications associated with venoarterial ECMO (VA-ECMO), including thrombus formation, bleeding, and hemolysis, remain significant challenges that impact patient outcomes and healthcare costs. These complications primarily arise from blood-material interactions within the ECMO circuit, necessitating the development of biocompatible materials to optimize hemocompatibility. This review provides an updated overview of the latest advancements in VA-ECMO materials, focusing on cannula, oxygenators, and centrifugal pumps. Various surface modifications, such as heparin coatings, nitric oxide-releasing polymers, phosphorylcholine (PC)-based coatings, and emerging omniphobic surfaces, have been explored to mitigate thrombosis and bleeding risks. Additionally, novel oxygenator membrane technologies, including zwitterionic polymers and endothelial-mimicking coatings, offer promising strategies to enhance biocompatibility and reduce inflammatory responses. In centrifugal pumps, magnetic levitation systems and hybrid polymer-composite impellers have been introduced to minimize shear stress and thrombogenicity. Despite these advancements, no single material has fully addressed all complications, and further research is needed to refine surface engineering strategies. This review highlights the current progress in ECMO biomaterials and discusses future directions in developing more effective and durable solutions to improve patient safety and clinical outcomes.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 6","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential Applications of PRP-Enhanced Polybutylene Succinate Graft as Vascular Access for Chemotherapy in Oncological Patients: A Systematic Review.","authors":"Andrea Gottardo, Giulia Bonventre, Tancredi Didier Bazan Russo, Pietro Zanatta, Giulia Lo Monte, Valerio Gristina, Antonio Galvano, Antonio Russo, Attilio Ignazio Lo Monte","doi":"10.3390/jfb16060228","DOIUrl":"10.3390/jfb16060228","url":null,"abstract":"<p><p>This systematic review aimed to evaluate the potential of combining platelet-rich plasma (PRP) and polybutylene succinate (PBS) for the development of vascular grafts in patients undergoing chemotherapy. Relevant articles published in English or Italian were selected through a comprehensive search of MEDLINE (via PubMed) and the Cochrane Library. A total of ten screened articles and two additional relevant studies were included. The synthesis of results was conducted using digital tools, thoroughly reviewed by the authors. The quality assessment of the included studies revealed a medium-to-high risk of bias, with frequent limitations such as small sample sizes, experimental designs, and overall moderate to low methodological quality. Despite the heterogeneity of the findings, the available evidence suggests that radiocephalic graft placement and the use of PBS as a scaffold material, in combination with the growth factors contained in PRP, may improve clinical outcomes and reduce complications related to arteriovenous graft implantation. While promising, the current literature on this topic remains scarce and fragmented, underscoring the need for additional preclinical and clinical research. The proposed approach appears to hold potential for improving vascular access in oncology, but further in vivo validation is essential. This study received no external funding. Registration: PROSPERO ID CRD42025646724.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 6","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12193876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Impact of Different Fiber Placement Techniques on the Fracture Resistance of Premolars Restored with Direct Resin Composite, In Vitro Study.","authors":"Reham Hesham Ibrahim, Dina Wafik ElKassas, Sameh Mahmoud Nabih, Mennatallah Naguib Salem, Rasha Haridy","doi":"10.3390/jfb16060225","DOIUrl":"10.3390/jfb16060225","url":null,"abstract":"<p><p>Fiber-reinforced composites (FRCs) are recognized for enhancing the fracture resistance of structurally compromised teeth. However, the optimal orientation and placement of fibers in direct resin composite restorations remain under debate. This study aimed to evaluate the fracture resistance of maxillary premolars with mesio-occluso-distal (MOD) cavities restored using polyethylene fibers with different placement techniques, compared to conventional incremental composite restoration.</p><p><strong>Methods: </strong>Sixty intact maxillary premolars were randomly assigned to six groups (n = 10). Group 1: intact teeth (positive control); Group 2: MOD cavity without restoration (negative control); Group 3: MOD cavity restored with nanohybrid composite using the incremental technique; Group 4: polyethylene fiber placed on the pulpal floor; Group 5: fiber placed circumferentially along cavity walls (wall-papering technique); Group 6: fiber placed buccolingually in an occlusal groove (occlusal splinting). Fracture resistance was assessed using a universal testing machine. Failure mode was also analyzed.</p><p><strong>Results: </strong>Group 6 (occlusal splinting) exhibited the highest fracture resistance (1137.72 ± 316.20 N), significantly exceeding Group 3 (546.93 ± 59.89 N) and other fiber-reinforced groups (<i>p</i> < 0.05). Failure mode analysis revealed no significant differences between the fiber-reinforced groups and the intact teeth. Group 6 also had the highest percentage of restorable fractures (90%).</p><p><strong>Conclusions: </strong>Incorporating polyethylene fibers, especially through occlusal splinting, significantly improves fracture resistance in MOD-restored maxillary premolars. This technique may offer a promising alternative to conventional composite restorations in structurally weakened posterior teeth.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 6","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194444/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Influence of the Machining Drill and Direction of Rotation on the Surfaces of Ti6Al4V Dental Implants Subjected to Implantoplasty.","authors":"Esteban Padullés-Gaspar, Francisco Real-Voltas, Esteban Padullés-Roig, Miguel Punset, Guillermo Cabanes, Pablo Fernández, Javier Gil","doi":"10.3390/jfb16060224","DOIUrl":"10.3390/jfb16060224","url":null,"abstract":"<p><p>Implantoplasty is widely used to treat peri-implantitis by removing biofilms from Ti6Al4V dental implants using rotating drills. This study examined the effects of diamond and tungsten carbide drills, and rotation direction (clockwise/counterclockwise), on surface modification, corrosion behavior, and cytotoxicity. Machining was performed for one minute under a controlled load. Surface roughness, nanohardness, compressive residual stress, and wettability were evaluated, along with SEM and EDX microanalyses of the residues. Corrosion behavior was evaluated using potentiostatic and potentiodynamic tests in Hank's solution. Ion release was monitored over time, and fibroblast viability was tested using extracts at various dilutions. The higher abrasiveness of diamond drills leads to increases roughness from 0.22 mm (control) to 0.73 and 0.59 for diamond and tungsten carbide drills, respectively; in hardness from 2.2 GPa for the control to 4.8 and 3.9 GPa; and in residual compressive stress from -26 to -125 and -111 MPa, with diamond drills inducing more significant changes and producing more hydrophilic surfaces with contact angles around 54° in relation to 80° and 62° for the control and tungsten carbide, respectively. Tungsten carbide drills caused lower corrosion rates (0.0323 mm/year) than diamond drills (0.052 mm/year). In addition, we observed the presence of tungsten ion release. Cytotoxic effects on human fibroblasts were observed with both bur types, and were more pronounced with tungsten carbide, especially at lower dilutions. Only 1:10 dilutions maintained consistent cytocompatibility. The rotation direction showed no significant impact. These findings emphasize the critical influence of bur selection in implantoplasty on the biological response of surrounding tissues.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 6","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12193868/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Thermal Aging on Color Stability and Mechanical Properties of High-Density CAD/CAM Polymers Utilized for Provisional Restorations.","authors":"Rasha Alharthi, Ali Robaian Alqahtani, Abdullah Mohammed Alshehri, Abdulrahman Almalki, Heba Wageh Abozaed, Eman Mohamed Raffat Hussein, Tarek Ahmed Soliman","doi":"10.3390/jfb16060223","DOIUrl":"10.3390/jfb16060223","url":null,"abstract":"<p><strong>Background: </strong>This study evaluated and compared the effects of thermal aging on the color stability and mechanical properties of CAD/CAM polymers utilized for provisional restorations.</p><p><strong>Material and methods: </strong>Three CAD/CAM polymers in this study: CAD-Temp (CAT), Everest C-Temp (CT), and PEEK (PK). Forty specimens of each material were randomly assigned to two subgroups. Subgroup A was immersed in distilled water for 24 h, whereas Subgroup B was subjected to 5000 thermal cycles. The color stability, flexural strength (FS), survival probability, and microstructures were evaluated following thermal cycling. Data analysis was conducted utilizing two-way ANOVA along with Tukey's test.</p><p><strong>Results: </strong>The CAT (3.74 ± 0.39) and CT (3.51 ± 0.54) groups exhibited the highest color variations, while PEEK (2.95 ± 0.45) showed the lowest color change. The baseline groups showed that the CT group had the highest flexural strength value (<i>p</i> < 0.05). The flexural strength values of CAT and CT groups significantly decreased (<i>p</i> < 0.05) following thermal cycling. No significant decrease in FS was observed following thermal cycling in the Pk group (<i>p</i> = 0.16).</p><p><strong>Conclusions: </strong>The color measurement and flexural strength outcomes were significantly influenced by CAD/CAM materials and thermal cycling. The CT group demonstrated superior flexural strength compared to the other groups, both before and after thermal cycling. The PK group shows the lowest color change compared to other groups. Regardless of aging condition, C-Temp and PEEK materials recorded the highest survival probability, a 95% significance level compared to CAD-Temp.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 6","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194252/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced Osteogenesis and Antibacterial Properties of Ketoprofen-Loaded MgCu-MOF74-Coated Titanium Alloy for Bone Implant.","authors":"Ziqing Duan, Yifeng Yao, Jiamin Liu, Yanni Tan, Qingge Wang, Man Fang, Aqsa Kanwal, Shuqiao Cheng, Juan Huang, Hong Wu","doi":"10.3390/jfb16060222","DOIUrl":"10.3390/jfb16060222","url":null,"abstract":"<p><p>To address the dual clinical challenges of poor osseointegration and inadequate analgesia caused by postoperative infections in traditional titanium implants, this study proposes a multifunctional synergistic strategy based on metal-organic frameworks (MOFs). By integrating drug-controlled release and ionic microenvironment regulation, it constructs a titanium-based implant coating system with antibacterial and bone-regenerative properties. Ketoprofen, a drug with excellent analgesic properties, was loaded into MgCu-MOF74 powder, and the Ket@MgCu-MOF74 powder was successfully anchored onto the surface of the titanium alloy through dopamine-mediated adhesion. The maximum load of ketoprofen to MgCu-MOF74 is 18.55%, and it has a good controllable release effect. The results showed that MgCu-MOF74/Ti and Ket@MgCu-MOF74/Ti coatings enhanced osteogenic performance by promoting alkaline phosphatase activity, collagen secretion, and extracellular matrix mineralization. Additionally, the release of Mg²⁺ and Cu²⁺ created an alkaline environment, providing antibacterial properties. In summary, the MOF enabled the controlled release of ketoprofen, and the composite coating can improve osteogenic differentiation of osteoblasts and enhance the antibacterial properties of titanium alloy implants.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 6","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194423/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Biological Properties Assessment of 3D-Printed Hydroxyapatite-Polylactic Acid Scaffolds Intended for Bone Regeneration.","authors":"Eddy Shan, Cristina Chamorro, Ana Ferrández-Montero, Rosa M Martin-Rodriguez, Begoña Ferrari, Antonio Javier Sanchez-Herencia, Leire Virto, María José Marín, Elena Figuero, Mariano Sanz","doi":"10.3390/jfb16060218","DOIUrl":"10.3390/jfb16060218","url":null,"abstract":"<p><p>This study evaluated the biological performance in vitro of two 3D-printed hydroxyapatite (HA) and polylactic acid (PLA) composite scaffolds with two different infill densities (50% [HA-PLA50] and 70% [HA-PLA70]). Comparative analysis using MG-63 cell cultures evaluated the following: (1) integrity after exposure to various sterilization methods; (2) cell viability; (3) morphological characteristics; (4) cell proliferation; (5) cytotoxicity; (6) gene expression; and (7) protein synthesis. Ultraviolet radiation was the preferred sterilization method. Both scaffolds maintained adequate cell viability and proliferation over 7 days without significant differences in cytotoxicity. Notably, HA-PLA50 scaffolds demonstrated superior osteogenic potential, showing a significantly higher expression of collagen type I (COL1A1) and an increased synthesis of interleukins 6 and 8 (IL-6, IL-8) compared to HA-PLA70 scaffolds. While both scaffold types supported robust cell growth, the HA-PLA50 formulation exhibited enhanced bioactivity, suggesting a potential advantage for bone tissue engineering applications. These findings provide important insights for optimizing 3D-printed bone graft substitutes.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 6","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194503/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stress Distribution on Endodontically Treated Anterior Teeth Restored via Different Ceramic Materials with Varying Post Lengths Versus Endocrown-A 3D Finite Element Analysis.","authors":"Mai Soliman, Nawaf Almutairi, Ali Alenezi, Raya Alenezi, Amal Abdallah A Abo-Elmagd, Manal M Abdelhafeez","doi":"10.3390/jfb16060221","DOIUrl":"10.3390/jfb16060221","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to evaluate the stress distribution on endodontically treated anterior teeth restored using different restorative materials and different post lengths versus endocrowns employing finite element analysis (FEA).</p><p><strong>Methods: </strong>An extracted human central incisor tooth with a fully formed apex was scanned using high-resolution cone beam computed tomography (CBCT) to generate 3D finite element models. Six models of restorations of badly destructed central incisor were grouped according to the type of ceramic material and post length versus endocrown restorations. Group V-L: Vita Enamic, long post (10 mm intra-radicular), Group C-L: Celtra Duo, long post (10 mm intra-radicular), Group V-Sh: Vita Enamic, short post (3 mm intra-radicular), Group C-Sh: Celtra Duo, short post (3 mm intra-radicular), Group V-E: Vita Enamic endocrown (3 mm intra-radicular), and Group C-E: Celtra Duo endocrown (3 mm intra-radicular). A static load of 200 N was applied to the palatal surface at a 45 degree angle to the tooth's long axis. The maximum equivalent von Mises stress and maximum principal stress were analyzed at four locations: the finish line, coronal third of the root (12 mm from the apex), middle third of the root (8 mm from the apex), and apical third of the root (4 mm from the apex).</p><p><strong>Results: </strong>Group C-L exhibited the highest maximum VM stress and PS at the finish line, in addition to the highest maximum VM stress and PS at the root apical third, while group C-Sh reported the least maximum VM stress at the root apical third among the groups. All Celtra Duo groups reported higher maximum VM stress than the corresponding groups of Vita Enamic at the finish line and root coronal thirds. However, at the root middle and apical thirds, both materials recorded similar stresses.</p><p><strong>Conclusions: </strong>Short posts and Vita Enamic endocrowns showed minimal stress, especially at the finish line, while long posts increased stress and fracture risk. The findings support conservative restorations without posts, although clinical validation is needed to confirm their long-term effectiveness and safety.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 6","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194378/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrospinning of Bovine Split Hide Collagen and Collagen/Glycosaminoglycan for a Study of Stem Cell Adhesion and Proliferation on the Mats: Influence of Composition and Structural Morphology.","authors":"Todorka G Vladkova, Dilyana N Gospodinova, Peter D Dineff, Milena Keremidarska-Markova, Kamelia Hristova-Panusheva, Natalia Krasteva","doi":"10.3390/jfb16060219","DOIUrl":"10.3390/jfb16060219","url":null,"abstract":"<p><p>Electrospun collagen-based fibrous mats are of increasing interest for cell culture, regenerative medicine, and tissue engineering. The focus of this investigation is on the assessment of the electrospinning ability of bovine split hide collagen (BSHC), the effect of glycosaminoglycan (GAG) incorporation on the mats' structural morphology, and the impact on the adhesion and proliferation of human adipose-derived mesenchymal stem cells (hAD-MSCs). Electrospun mats were prepared using benign and fluoroalcohol solutions of BSHC and BSHC/GAGs under varied operation conditions. SEM observations and analysis were employed to characterize the structural morphology of the mats. Several parameters were used to evaluate the hAD-MSC behavior: cytotoxicity, cell morphology, cell number and spreading area, cytoskeleton, focal adhesion contacts, and cell proliferation. Electrospinning using benign solvents was impossible. However, fiber mats were successfully prepared from hexafluoropropanol (HFP) solutions. Different structural morphologies and fiber diameters of the electrospun mats were observed depending on the composition and concentration of the electrospinning solutions. Both BSHC and BSHC/GAG mats supported the in vitro adhesion, growth, and differentiation of hAD-MSCs, with some variations based on their composition and structural morphology. The absence of cytotoxicity and the good hAD-MSC adhesiveness make them promising substrates for cell adhesion, proliferation, and further stem cell differentiation.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 6","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}