Journal of Functional Biomaterials最新文献

{"title":"Evaluating Bioassays for the Determination of Simvastatin's Osteogenic Activity: A Systematic Review.","authors":"Lara Steiner Back, Isabella Schönhofen Manso, Mariane Beatriz Sordi, Gabriel Leonardo Magrin, Águedo Aragonês, Ricardo de Souza Magini, Reinhard Gruber, Ariadne Cristiane Cabral Cruz","doi":"10.3390/jfb16020061","DOIUrl":"10.3390/jfb16020061","url":null,"abstract":"<p><strong>Objective: </strong>Osteogenic differentiation is a complex process, and its analysis requires several biomarkers. Allied with this, there are no standardized bioassays to monitor the activity of simvastatin in osteogenesis in vitro. Therefore, identifying the most efficient and sensitive bioassays may enhance the quality of in vitro studies, bridging the gap with in vivo findings, saving time and resources, and benefiting the community. This systematic review aimed to determine the most efficient bioassay for simvastatin's osteogenic activity in vitro, in terms of sensitivity.</p><p><strong>Materials and methods: </strong>In vitro studies evaluating undifferentiated mesenchymal cells treated with simvastatin were considered eligible. References were selected in a two-phase process. Electronic databases and the grey literature were screened up to September 2023. The Office of Health Assessment and Translation (OHAT) tool was used to assess the risk of bias. Certainty in cumulative evidence was evaluated using the Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) criteria. Data were analyzed considering extracellular matrix mineralization, alkaline phosphatase, and the expression of potential target genes, such as bone morphogenetic protein-2 (BMP-2), collagen type I, Runt-related transcription factor 2, osterix, osteocalcin, and osteopontin.</p><p><strong>Results: </strong>Fourteen studies were included. A \"probably low\" or a \"definitely low\" risk of bias was assigned to the included studies. The simvastatin concentration ranged from 0.1 nM to 10 µM. Considering a minimum 4-fold increase, simvastatin caused robust mineralization of the extracellular matrix in four studies (4.0-, 4.4-, 5.0-, and 39.5-fold). Moreover, simvastatin substantially increased BMP-2 expression in mesenchymal cells in three studies (4-, 11-, and 19-fold).</p><p><strong>Conclusion: </strong>Therefore, mineralization of the extracellular matrix and BMP-2 expression in mesenchymal cells are the most efficient bioassays for determining the osteogenic activity of simvastatin in vitro (high certainty level). These findings provide a standardized approach that can enhance the reliability and comparability of in vitro studies, bridging the gap with in vivo research and optimizing resources in the field of bone regeneration.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 2","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors Affecting the Color Change of Monolithic Zirconia Ceramics: A Narrative Review.","authors":"Ebru Binici Aygün, Esra Kaynak Öztürk, Ayşe Bilge Tülü, Bilge Turhan Bal, Seçil Karakoca Nemli, Merve Bankoğlu Güngör","doi":"10.3390/jfb16020058","DOIUrl":"10.3390/jfb16020058","url":null,"abstract":"<p><p>Zirconia restorations are widely used in dentistry due to their high esthetic expectations and physical durability. However, zirconia's opaque white color can compromise esthetics. Therefore, zirconia is often veneered with porcelain, but fractures may occur in the veneer layer. Monolithic zirconia restorations, which do not require porcelain veneering and offer higher translucency, have been developed to address this issue. Zirconia exists in three main crystal phases: monoclinic, tetragonal, and cubic. Metal oxides such as yttrium are added to stabilize the tetragonal phase at room temperature. 3Y-TZP contains 3 mol% yttrium and provides high mechanical strength but has poor optical properties. Recently, 4Y-PSZ and 5Y-PSZ ceramics, which offer better optical properties but lower mechanical strength, have been introduced. This review examines the factors affecting the color change in monolithic zirconia ceramics. These factors are categorized into six main groups: cement type and color, restoration thickness, substrate color, sintering, aging, and zirconia type. Cement type and color are crucial in determining the final shade, especially in thin restorations. Increased restoration thickness reduces the influence of the substrate color while the sintering temperature and process improve optical properties. These findings emphasize the importance of material selection and application processes in ensuring esthetic harmony in zirconia restorations. This review aims to bridge gaps in the literature by providing valuable insights that guide clinicians in selecting and applying zirconia materials to meet both esthetic and functional requirements in restorative dentistry.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 2","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11855960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Personalized 3D-Printed Prostheses for Bone Defect Reconstruction After Tumor Resection in the Foot and Ankle.","authors":"Chang-Jin Yon, Byung-Chan Choi, Jung-Min Lee, Si-Wook Lee","doi":"10.3390/jfb16020062","DOIUrl":"10.3390/jfb16020062","url":null,"abstract":"<p><p>Three-dimensional (3D)-printing technology is revolutionizing orthopedic oncology by providing precise, customized solutions for complex bone defects following tumor resection. Traditional modular endoprostheses are prone to complications such as fretting corrosion and implant failure, underscoring the need for innovative approaches. This case series reports on three patients treated with 3D-printed, patient-specific prostheses and cutting guides. Preoperative CT and MRI data were used to design implants tailored to each patient's anatomy, manufactured using electron beam melting technology with a titanium-aluminum-vanadium alloy. Functional outcomes showed significant improvements: in Case I, AOFAS improved from 71 to 96, and VAS decreased from 6 to 1; in Case II, AOFAS increased from 65 to 79, and VAS decreased from 5 to 3. Radiographic evaluations demonstrated stable prosthesis placement and early evidence of bone integration in Cases I and II, while in Case III, localized disease control was achieved before systemic progression. This case series highlights the transformative potential of 3D-printed prostheses in addressing the challenges of reconstructing anatomically complex defects. By enabling precise tumor resection and improving functional outcomes, this approach can advance current practices in orthopedic oncology. Further research should explore larger cohorts and use cost-effectiveness analyses to validate these findings and facilitate broader clinical adoption.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 2","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856901/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dental Implant Survival Rates: Comprehensive Insights from a Large-Scale Electronic Dental Registry.","authors":"Guy Tobias, Tali Chackartchi, Doron Haim, Jonathan Mann, Mordechai Findler","doi":"10.3390/jfb16020060","DOIUrl":"10.3390/jfb16020060","url":null,"abstract":"<p><strong>Background: </strong>This descriptive study aimed to assess the survival rates and outcomes of dental implants in one of the four national HMOs in Israel. Data are provided for the period from 1 January 2014 to 31 December 2022.</p><p><strong>Materials and methods: </strong>This retrospective analysis utilized electronic medical records of patients who underwent dental implant placement during the specified period. Statistical analyses included chi-squared tests, Student's <i>t</i>-tests, and generalized estimating equation (GEE) analyses to identify potential risk factors associated with early and late implant failures.</p><p><strong>Results: </strong>A total of 158,824 dental implants were placed in 53,874 patients. The overall implant failure rate was 2.21%, while the early failure rate during the osseointegration phase-before prosthetic reconstruction-was 1.56%. Significant associations with implant failure were observed for male patients (2.53% failure rate), implants in the maxillary molar region (3%), and the central incisor region (3.37%), approximately double the failure rates seen in other implant sites (<i>p</i> < 0.001).</p><p><strong>Conclusions: </strong>This extensive data analysis demonstrates a low overall failure rate for dental implants. The highest failure incidence occurred within the first year post-implantation, declining in subsequent years irrespective of rehabilitation status. Early failure risk factors differ based on various factors and should be carefully integrated into presurgical planning.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 2","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of PVA Coatings Applied to Electrospun PLGA Scaffolds to Prevent Postoperative Adhesions.","authors":"Arsalan D Badaraev, Evgenii V Plotnikov, Vladislav R Bukal, Gleb E Dubinenko, Johannes Frueh, Sven Rutkowski, Sergei I Tverdokhlebov","doi":"10.3390/jfb16020057","DOIUrl":"10.3390/jfb16020057","url":null,"abstract":"<p><p>There is currently a demand for anti-adhesive materials that are capable of preventing the formation of intra-abdominal adhesions. In this study, electrospun poly(lactide-co-glycolide) scaffolds were dip-coated in aqueous solutions of polyvinyl alcohol with concentrations of 3 wt.%, 6 wt.% and 9 wt.% to obtain a nontoxic and anti-adhesive biomedical material. The viscosities of the applied 3 wt.%, 6 wt.% and 9 wt.% polyvinyl alcohol solutions were 7.7 mPa∙s, 38.2 mPa∙s and 180.8 mPa∙s, respectively, and increased exponentially. It is shown that increasing the viscosity of the polyvinyl alcohol solution from 6 wt.% to 9 wt.% increases the thickness of the polyvinyl alcohol layer from (3.32 ± 0.97) µm to (8.09 ± 1.43) µm. No pronounced polyvinyl alcohol layer can be observed on samples dip-coated in 3 wt.% PVA solution. Increasing the viscosity of the polyvinyl alcohol solution from 3 wt.% to 9 wt.% increases the mechanical properties of the poly(lactide-co-glycolide) samples by a factor of 1.16-1.45. Cytotoxicity analysis of all samples reveals that none is toxic to 3T3-L1 fibroblast cells. A cell adhesion assay indicates that the anti-adhesion properties increase with increasing viscosity of the polyvinyl alcohol solution and the thickness of the polyvinyl alcohol layer on the poly(lactide-co-glycolide) scaffolds. Fluorescence images of the cells show that as the thickness of the polyvinyl alcohol coating increases, the number of cells decreases, and they do not cover the surface of the samples and form spherical three-dimensional agglomerates. The highest mechanical and anti-adhesion properties are obtained with the poly(lactide-co-glycolide) scaffold sample dip-coated in the 9 wt.% polyvinyl alcohol solution. This is because this sample has the thickest polyvinyl alcohol coating.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 2","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epigallocatechin-3-Gallate (EGCG)-Loaded Hyaluronic Acid Hydrogel Seems to Be Effective in a Rat Model of Collagenase-Induced Achilles Tendinopathy.","authors":"Hwa Jun Kang, Sivakumar Allur Subramanian, Si Young Song, Jihyun Hwang, Collin Lee, Sung Jae Kim","doi":"10.3390/jfb16020055","DOIUrl":"10.3390/jfb16020055","url":null,"abstract":"<p><p>Tendon injuries account for 45% of musculoskeletal injuries. However, research on the occurrence and pathogenesis of tendinopathy is insufficient, and there is still much debate regarding treatment methods. It is important to understand the molecular mechanisms of oxidative stress and inflammatory responses because oxidative stress in tendon tissue is induced by various factors, including inflammatory cytokines, drug exposure, and metabolic abnormalities. In this study, 28 rats were divided into four groups (7 rats assigned to each group): control group (CON), collagenase injection group (CL), collagenase injection and hyaluronic acid injection group (CL + HA), and collagenase injection and EGCG-loaded hyaluronic acid injection group (CL + HA + EGCG). Seven weeks after the start of the study, all rats underwent histochemical analysis, immunofluorescence staining, and Western blot. The results showed increased inflammatory cells, disarray of collagen matrix, and degradation of the collagen matrix in the CL group. However, in the EGCG-treated group, there was a significant increase in type I collagen expression and a significant decrease in type III collagen expression, compared to the CL group. Additionally, there was an increase in the expression of antioxidant markers SOD (Superoxide Dismutase) and CAT (Catalase), tenogenic markers COLL-1 (collagen type I), and SCX (Scleraxis), and a downregulated expression of apoptosis markers cas-3 and cas-7. Our findings suggest that EGCG-loaded hyaluronic acid hydrogel exhibits potential in preventing tendon damage and promoting the regeneration process in a rat model of Achilles tendinopathy. The insights gained from our histological and molecular investigations highlight the future potential for testing novel tendinopathy treatments in human subjects.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 2","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856759/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrospun Silk Fibroin-Silk Sericin Scaffolds Induced Macrophage Polarization and Vascularization for Volumetric Muscle Loss Injury.","authors":"Yuqing Wang, Fangyu Ye, Xinbo Wei, Manman Wang, Zheng Xing, Haifeng Liu","doi":"10.3390/jfb16020056","DOIUrl":"10.3390/jfb16020056","url":null,"abstract":"<p><p>Volumetric muscle loss (VML) results in the impediment of skeletal muscle function. Tissue engineering scaffolds have been widely developed and used in skeletal muscle regeneration. However, scaffold implantation causes an immune response that endogenously regulates implant integration and tissue regeneration. Moreover, vascularization is thought to be a principal obstacle in the reconstruction of skeletal muscle defects. Thus, creating a pro-regenerative microenvironment that facilitates muscle regeneration and supports angiogenesis represents a promising strategy for tissue repair following volumetric muscle loss (VML) injury. Previously, the electrospun silk fibroin-silk sericin (SF-SS) film could regulate macrophage polarization and promote neovessel formation. This study aimed to investigate if the electrospun SF-SS scaffold was capable of supporting functional muscle regeneration. The results indicate that the conditioned medium collected from macrophages co-cultured with the 7:3 SF-SS scaffold significantly enhanced the proliferation and migration of myoblast C2C12 cells and improved the tube formation of HUVECs. Data from animal studies showed that the 7:3 SF-SS scaffold significantly enhanced M2 macrophage polarization, vascularization, and muscle fiber regeneration, reduced fibrosis, and improved muscle function after VML injury, thereby promoting the repair of muscle tissue. Therefore, the 7:3 SF-SS scaffold might represent a potential candidate for skeletal muscle regeneration following VML injury.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 2","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856479/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hybrid Biomechanical Design of Dental Implants: Integrating Solid and Gyroid Triply Periodic Minimal Surface Lattice Architectures for Optimized Stress Distribution.","authors":"Dawit Bogale Alemayehu, Masahiro Todoh, Song-Jeng Huang","doi":"10.3390/jfb16020054","DOIUrl":"10.3390/jfb16020054","url":null,"abstract":"<p><strong>Background: </strong>Dental implantology has evolved significantly since the introduction of additive manufacturing, which allows for the reproduction of natural bone's porous architecture to improve bone tissue compatibility and address stress distribution issues important to long-term implant success. Conventional solid dental implants frequently cause stress shielding, which compromises osseointegration and reduces durability.</p><p><strong>Aim: </strong>The current research proposes to examine the biomechanical efficacy of fully and hybrid gyroid triply periodic minimum surface (TPMS) latticed implants across different cell sizes to optimize stress distribution and improve implant durability.</p><p><strong>Methods: </strong>This study evaluates six fully and hybrid gyroid (TPMS) latticed implants, including fully latticed designs with three cell sizes-FLI_111 (1 mm × 1 mm × 1 mm), FLI_222 (2 mm × 2 mm × 2 mm), and FLI_333 (3 mm × 3 mm × 3 mm)-and hybrid gyroid TPMS latticed implants with solid necks in corresponding sizes-HI_111, HI_222, and HI_333. To enhance initial stability, a square-threaded design was added into the bottom part of both fully and hybrid lattice implants. The designs also incorporate anti-rotational connections to enhance fixation, and they undergo a clinical viability comparison with contemporary implants. To improve lattice designs, finite element analysis (FEA) was utilized through nTopology (nTOP 4.17.3) to balance stiffness and flexibility. To examine mechanical performance under realistic conditions, a dynamic mastication loading simulation was conducted for 1.5 s across three cycles.</p><p><strong>Results: </strong>The findings reveal that hybrid implants, particularly HI_222, exhibited improved mechanical characteristics by reducing micromotions at the bone-implant interface, improving osteointegration, and attaining better stress distribution.</p><p><strong>Conclusions: </strong>By addressing stress shielding and boosting implant performance, this work paves the way for personalized implant designs, developing dental technology, and improving clinical results.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 2","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856169/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Processing Parameters on Additively Manufactured Architected Cellular Metals: Emphasis on Biomedical Applications.","authors":"Yixuan Shi, Yuzhe Zheng, Chengcong Huang, Shangyan Zhao, Xuan Li, Yuchen Lu, Yuzhi Wu, Peipei Li, Luning Wang, Yageng Li","doi":"10.3390/jfb16020053","DOIUrl":"10.3390/jfb16020053","url":null,"abstract":"<p><p>Laser powder bed fusion (LPBF) has emerged as a transformative additive manufacturing technique for fabricating architected cellular metallic structures, offering tailored properties for diverse biomedical applications. These structures are particularly well-suited for bone implants, scaffolds, and other load-bearing medical devices due to their ability to achieve lightweight designs, enhanced mechanical properties, and customized geometries. However, the complex interactions between LPBF process parameters and the resulting structural and mechanical properties pose significant challenges in achieving the precision and reliability required for clinical applications. This review provides a comprehensive analysis of the effects of LPBF process parameters, including laser power, scanning speed, and layer thickness, on key attributes such as dimensional accuracy, density, surface roughness, and microstructure. Their influence on the mechanical performance, including strength, fatigue resistance, and functional properties, is critically examined, with specific attention to biomedical relevance. The impact of lattice design factors, such as topology, unit cell size, and orientation, is also discussed, underscoring their role in optimizing biocompatibility and structural integrity for medical applications. Challenges such as surface defects, geometric inaccuracies, and microstructural inconsistencies are highlighted as key barriers to the broader adoption of LPBF in biomedical fields. Future perspectives focus on advancing LPBF technologies through process optimization and integration with advanced computational tools, such as machine learning, to enable efficient manufacturing of complex, patient-specific architectures. By addressing these challenges, LPBF has the potential to revolutionize the development of next-generation biomaterials, tailored to meet evolving clinical needs and improve patient outcomes.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 2","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856420/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Next-Generation Biomaterials for Wound Healing: Development and Evaluation of Collagen Scaffolds Functionalized with a Heparan Sulfate Mimic and Fibroblast Growth Factor 2.","authors":"Merel Gansevoort, Sabine Wentholt, Gaia Li Vecchi, Marjolein de Vries, Elly M M Versteeg, Bouke K H L Boekema, Agnes Choppin, Denis Barritault, Franck Chiappini, Toin H van Kuppevelt, Willeke F Daamen","doi":"10.3390/jfb16020051","DOIUrl":"10.3390/jfb16020051","url":null,"abstract":"<p><p>Fibrosis after full-thickness wound healing-especially after severe burn wounds-remains a clinically relevant problem. Biomaterials that mimic the lost dermal extracellular matrix have shown promise but cannot completely prevent scar formation. We present a novel approach where porous type I collagen scaffolds were covalently functionalized with ReGeneRating Agent (RGTA^®) OTR4120. RGTA^® is a glycanase-resistant heparan sulfate mimetic that promotes regeneration when applied topically to chronic wounds. OTR4120 is able to capture fibroblast growth factor 2 (FGF-2), a heparan/heparin-binding growth factor that inhibits the activity of fibrosis-driving myofibroblasts. Scaffolds with various concentrations and distributions of OTR4120 were produced. When loaded with FGF-2, collagen-OTR4120 scaffolds demonstrated sustained release of FGF-2 compared to collagen-heparin scaffolds. Their anti-fibrotic potential was investigated in vitro by seeding primary human dermal fibroblasts on the scaffolds followed by stimulation with transforming growth factor β1 (TGF-β1) to induce myofibroblast differentiation. Collagen-OTR4120(-FGF-2) scaffolds diminished the gene expression levels of several myofibroblast markers. In absence of FGF-2 the collagen-OTR4120 scaffolds displayed an inherent anti-fibrotic effect, as the expression of two fibrotic markers (TGF-β1 and type I collagen) was diminished. This work highlights the potential of collagen-OTR4120 scaffolds as biomaterials to improve skin wound healing.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 2","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11856099/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}