Journal of Functional Biomaterials最新文献_第2页

Clinical and Radiological Evaluation of Flap and Flapless Procedures with Biomaterials in Alveolar Ridge Preservation. 生物材料皮瓣和无皮瓣在牙槽嵴保存中的临床和放射学评价。

IF 5.2 3区医学

Journal of Functional Biomaterials Pub Date : 2025-09-14 DOI: 10.3390/jfb16090345

Ewa Dolińska, Ewa Duraj, Marcin Bernaczyk, Magdalena Sulewska, Małgorzata Pietruska

{"title":"Clinical and Radiological Evaluation of Flap and Flapless Procedures with Biomaterials in Alveolar Ridge Preservation.","authors":"Ewa Dolińska, Ewa Duraj, Marcin Bernaczyk, Magdalena Sulewska, Małgorzata Pietruska","doi":"10.3390/jfb16090345","DOIUrl":"10.3390/jfb16090345","url":null,"abstract":"Although ridge preservation procedures have been shown to prevent post-extraction bone loss, the effectiveness of using a flap or flapless surgical approach remains unclear. The aim of the study was to compare the mentioned above alveolar ridge preservation procedures in the esthetic region of maxilla. Twenty-nine patients were randomly assigned to receive flap (n = 14) or flapless (n = 15) alveolar ridge preservation procedure. Sockets were grafted with alloplastic biomaterial, then covered with a collagen membrane in both groups. Clinical examinations were performed over a 6-month observation period and radiological (CBCT) examination was conducted before and 6 months after treatment. For both after flap and flapless procedures, there was a reduction in interdental papillae height and keratinized tissue width, increase in buccal soft tissues thickness with a decrease in radiological buccal bone plate width, decrease in radiological buccal and lingual plate height (significantly for the flapless group) and radiological alveolar process width reduction (significantly in flapless group at the height of 5 mm and 7 mm from the bottom of the socket). A decrease in the radiological buccal bone plate width was observed, where the further measuring point was from the bottom of the alveolus. In the mucoperiosteal flap preparation, group buccal bone plate width reduction at the height 3 mm, 5 mm and 7 mm was significant and in the flapless group a significant decrease was observed from 5 to 9 mm from the bottom of the socket. Despite ridge preservation, there is soft tissue thickening and a decrease in bone height and width regardless of the flap/flapless method used.","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 9","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470390/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial Activity of a Trace-Cu-Modified Mg Alloy in Simulated Intestinal Fluid. 微量cu修饰镁合金在模拟肠液中的抗菌活性

IF 5.2 3区医学

Journal of Functional Biomaterials Pub Date : 2025-09-12 DOI: 10.3390/jfb16090344

Baiyun Zhong, Zemeng Wei, Yi Yao, Lixun Jiang, Manli Zhou, Jinping Li, Weidong Liu, Xin Li, Ming-Chun Zhao

{"title":"Antibacterial Activity of a Trace-Cu-Modified Mg Alloy in Simulated Intestinal Fluid.","authors":"Baiyun Zhong, Zemeng Wei, Yi Yao, Lixun Jiang, Manli Zhou, Jinping Li, Weidong Liu, Xin Li, Ming-Chun Zhao","doi":"10.3390/jfb16090344","DOIUrl":"10.3390/jfb16090344","url":null,"abstract":"Mg alloys hold promise for biodegradable gastrointestinal implants, but most evaluations rely on simplified media like Hank's solution, which lacks organic components and fails to replicate the acidic-to-alkaline transition of intestinal fluid, risking underestimation of biodegradation rates and clinical relevance. This work investigated a trace-Cu-modified Mg alloy (Mg-0.05Cu) in simulated intestinal fluid (SIF) versus Hank's solution. Microstructural analysis confirmed Mg2Cu intermetallic phases as Cu reservoirs. Electrochemical and immersion tests revealed significantly accelerated biodegradation in SIF, due to its disruption of protective layer formation, sustaining loose biodegradation products. The biodegradation rate of the trace-Cu-modified Mg alloy in SIF was consistent with reported values for Mg alloys in similar media, as was that in Hank's solution. Remarkably, Mg-0.05Cu exhibited potent antibacterial activity against E. coli, achieving 99.3% eradication within 12 h and 100% elimination by 24-48 h, alongside excellent cytocompatibility with L929 cells (>95% viability). This efficacy arose from the synergistic Cu2+ release and high-pH microenvironment. These findings demonstrate that trace Cu alloying in high-purity Mg balances rapid antibacterial action with controlled biodegradation in a physiologically relevant SIF. This positions Mg-0.05Cu as a highly promising candidate for practical applications, such as biodegradable intestinal stents, anti-adhesion barriers, anastomosis rings, and anti-obesity devices, where rapid infection control and predictable degradation are critical for clinical success. This work underscores the importance of using biomimetic media for evaluating gastrointestinal implants and establishes Mg-0.05Cu as a promising strategy for developing infection-resistant biodegradable devices.","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 9","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrophoretic Coatings for Orthodontic Implants: Evaluation of Surface Properties, Adhesion, and Antibacterial Activity in Simulated Implantation Trials. 正畸种植体的电泳涂层：模拟种植试验中表面特性、粘附性和抗菌活性的评估。

IF 5.2 3区医学

Journal of Functional Biomaterials Pub Date : 2025-09-12 DOI: 10.3390/jfb16090343

Maria Biegun-Żurowska, Karolina Klesiewicz, Katarzyna Matysiak, Marcin Gajek, Alicja Rapacz-Kmita, Magdalena Ziąbka

{"title":"Electrophoretic Coatings for Orthodontic Implants: Evaluation of Surface Properties, Adhesion, and Antibacterial Activity in Simulated Implantation Trials.","authors":"Maria Biegun-Żurowska, Karolina Klesiewicz, Katarzyna Matysiak, Marcin Gajek, Alicja Rapacz-Kmita, Magdalena Ziąbka","doi":"10.3390/jfb16090343","DOIUrl":"10.3390/jfb16090343","url":null,"abstract":"In this study, the properties of electrophoretically deposited (EPD) coatings on orthodontic implants made from Ti-6Al-4V alloy were evaluated during simulated implantation trials on animal bones. Three types of chitosan-based coatings were prepared using EPD: titanium nitride microparticles (TiNPs), titanium nitride nanoparticles (TiNNPs), and boron nitride particles (BNPs). Each of these coatings was also modified by adding a polylactic acid (PLA) layer using a dip-coating technique to compare their properties with and without this additional layer. The coatings were analysed using optical microscopy, confocal microscopy, and scanning electron microscopy (SEM) with elemental analysis. Surface roughness measurements of the coated implants were also conducted to highlight differences that could significantly influence the type and strength of the bone-implant interface, directly affecting the stability of the implant as an anchorage unit. Eventually, to evaluate the antibacterial properties of the EPD coatings, their antibacterial activity against both Gram-positive and Gram-negative bacteria strains was tested. Scanning electron observations confirmed the homogenous distribution of micro- and nanoparticles in all coatings. The highest surface roughness values were observed in layers containing titanium nitride nanoparticles (TiNNPs) and chitosan. The presence of an additional dip-coating PLA layer improved the adhesion, and its effect on the surface roughness depended on the particle size. While the antibacterial properties of the coatings show promising results, achieving optimal adhesion of the coatings to implants remains a challenge that requires further development.","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 9","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470570/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of Design Parameters on Implant Abutment Performance: A Scoping Review. 设计参数对种植体基台性能影响的研究综述。

IF 5.2 3区医学

Journal of Functional Biomaterials Pub Date : 2025-09-11 DOI: 10.3390/jfb16090342

Vladimir Prpic, Petar Kosec, Stanko Skec, Amir Catic

{"title":"Influence of Design Parameters on Implant Abutment Performance: A Scoping Review.","authors":"Vladimir Prpic, Petar Kosec, Stanko Skec, Amir Catic","doi":"10.3390/jfb16090342","DOIUrl":"10.3390/jfb16090342","url":null,"abstract":"Implant abutments serve as the critical interface between dental implants and prosthodontic restorations, playing a central role in the functional and aesthetic success of implant-supported rehabilitations. With the development of CAD/CAM technologies, dental implantology has experienced a significant transformation. The latest and still unexplored field of science includes an advanced algorithm-driven process known as generative design. Generally, generative design is used to investigate a broad spectrum of design alternatives that satisfy predetermined criteria established by a designer. Prior to the application of generative design, it is essential to define the key parameters that influence the optimization of the designed object's configuration, such as a hybrid implant abutment. A bibliographic search was performed using PubMed and Scopus databases to identify relevant studies published up to 1 July 2025. Studies that investigated transmucosal and prosthodontic height of implant abutments were selected for inclusion. Only 13 studies met inclusion criteria and were further analyzed. Included studies showed the importance of transmucosal and prosthodontic height of implant abutments in order to reduce possible complications. The review highlights the importance of optimizing the transmucosal and transgingival heights of implant abutments. For predictable biological and mechanical outcomes, both dimensions should be at least 2 mm. The obtained parameter values will be used to create the initial parametric model, which will then be utilized in the generative design process of hybrid implant abutments.","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 9","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Types of Bone Substitutes and Their Application in Regenerative Medicine: A Systematic Review. 骨替代品的种类及其在再生医学中的应用：系统综述。

IF 5.2 3区医学

Journal of Functional Biomaterials Pub Date : 2025-09-09 DOI: 10.3390/jfb16090341

Nikoleta Ivanova, Stoyan Ivanov, Stefan Peev, Tsanka Dikova

{"title":"Types of Bone Substitutes and Their Application in Regenerative Medicine: A Systematic Review.","authors":"Nikoleta Ivanova, Stoyan Ivanov, Stefan Peev, Tsanka Dikova","doi":"10.3390/jfb16090341","DOIUrl":"10.3390/jfb16090341","url":null,"abstract":"Background: The growing demand for effective methods of bone tissue regeneration highlights the relevance of studying modern bone substitutes and their applications in regenerative medicine. The aim of this work was to conduct a comprehensive analysis of the biological, mechanical, and clinical characteristics of various types of bone substitutes to determine their potential in regenerative medicine.Methods: The study was performed as a systematic literature review in accordance with PRISMA guidelines, analyzing 68 high-quality scientific sources from 2019 to May 2025, using the PubMed, Scopus, Web of Science, and Google Scholar databases.Results: It was established that autogenous grafts exhibit the highest osteogenic properties due to the presence of growth factors BMP-2, BMP-7, and concentrated growth factors; however, their use is limited by donor site morbidity in 20-30% of patients and the requirement to treat 6% of fractures complicated by non-union. Allogeneic and xenogeneic substitutes provide structural support for large defects but require intensive processing in accordance with European Directives 2004/23/EC and 2006/86/EC to minimize the risk of infection transmission. Synthetic substitutes based on calcium phosphate ceramics with pore sizes ranging from 23 to 210 micrometres demonstrate excellent biocompatibility and controlled degradation, with β-tricalcium phosphate exhibiting optimal characteristics for long-term applications compared to calcium sulphate.Conclusions: The findings of the study highlight the necessity of a personalized approach in selecting bone substitutes, considering the specific requirements of medical specialities, and support the development of hybrid biomaterials to combine structural strength with biological activity.","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 9","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470451/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of 45S5-Bioactive Glass on Chondrocytes in Knee Osteoarthritis-In Vitro Study Exploring Cellular Responses. 45s5生物活性玻璃对膝关节骨关节炎软骨细胞影响的体外细胞反应研究

IF 5.2 3区医学

Journal of Functional Biomaterials Pub Date : 2025-09-09 DOI: 10.3390/jfb16090339

Max Marinescu, Sébastien Hagmann, Jörg Fellenberg, Elena Tripel, Simone Gantz, Ravikumar Mayakrishnan, Aldo R Boccaccini, Tobias Renkawitz, Babak Moradi, Fabian Westhauser, Hadrian Platzer

{"title":"Impact of 45S5-Bioactive Glass on Chondrocytes in Knee Osteoarthritis-In Vitro Study Exploring Cellular Responses.","authors":"Max Marinescu, Sébastien Hagmann, Jörg Fellenberg, Elena Tripel, Simone Gantz, Ravikumar Mayakrishnan, Aldo R Boccaccini, Tobias Renkawitz, Babak Moradi, Fabian Westhauser, Hadrian Platzer","doi":"10.3390/jfb16090339","DOIUrl":"10.3390/jfb16090339","url":null,"abstract":"Osteoarthritis (OA), the most common joint disease, is marked by cartilage degradation and chronic inflammation. While 45S5-bioactive glass (45S5-BG) is well-established in bone regeneration and has been suggested to exert immunomodulatory effects, its impact on OA chondrocytes remains largely unexplored. Therefore, this in vitro study investigated the effects of 45S5-BG microparticles (0.125 mg/mL) on chondrocytes derived from OA patients, evaluating its therapeutic potential in OA. Chondrocytes were cultured with or without 45S5-BG for 1 and 7 days. Gene expression of cartilage markers, cytokines, matrix metalloproteinases (MMPs), and toll-like receptors (TLRs) was analyzed by qPCR. Protein levels were assessed by ELISA. 45S5-BG stimulation significantly altered chondrocyte activity, inducing upregulation of IL-6, IL-1β, TNF-α, MMP-1/-3/-13, and TLR4. Expression of ACAN and COL2A1 was reduced, while COL10A1-a marker of chondrocyte hypertrophy-was significantly increased at day 1. These findings show a catabolic and pro-inflammatory shift in chondrocyte phenotype upon 45S5-BG exposure, showing no therapeutic benefit of 45S5-BG on OA chondrocytes. However, considering the pronounced effects on chondrocyte activity and the well-established bioactivity and biocompatibility of 45S5-BG, our findings suggest that modified BG formulations could be developed to enhance chondroprotective and anti-inflammatory properties, warranting further investigation in co-culture and in vivo models.","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 9","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modified Polysaccharides: Potential Biomaterials for Bioprinting. 改性多糖：生物打印的潜在生物材料。

IF 5.2 3区医学

Journal of Functional Biomaterials Pub Date : 2025-09-09 DOI: 10.3390/jfb16090338

Tao Jiang, Yun Yang, Zening Lin, Yang Hong, Zirong Luo

{"title":"Modified Polysaccharides: Potential Biomaterials for Bioprinting.","authors":"Tao Jiang, Yun Yang, Zening Lin, Yang Hong, Zirong Luo","doi":"10.3390/jfb16090338","DOIUrl":"10.3390/jfb16090338","url":null,"abstract":"Polysaccharides have emerged as promising biomaterials for 3D bioprinting due to their inherent biocompatibility, biodegradability, and structural diversity. However, their limited mechanical strength, insufficient bioactivity, and suboptimal printability hinder their direct application in fabricating complex tissue constructs. This review systematically summarizes universal modification strategies to address these challenges by tailoring polysaccharides' physicochemical and biological properties. We first analyse the fundamental requirements of bioprinting materials, emphasising on the critical role of shear-thinning behaviours, post-printing structural fidelity, and cell-instructive functions. Subsequently, we highlight the advantages and limitations of representative polysaccharides, including chitosan, alginate, and hyaluronic acid. Chemical functionalisation, physical reinforcement, and biological hybridisation are proposed as versatile approaches to synergistically enhance printability, mechanical robustness, and bioactivity to tackle the limitations. Furthermore, dynamic crosslinking mechanisms enabling self-healing and stimuli-responsive behaviours are discussed as emerging solutions for constructing biomimetic architectures. Finally, we outline future directions in balancing material processability with cellular viability and scaling up modified polysaccharides for clinical translation. This review aims to provide a design blueprint for engineering polysaccharide-based bioinks toward next-generation regenerative medicine.","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 9","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Evaluation of the Bone Regenerative Potential of a Novel Calcium Silicate-Modified Calcium Carbonate Graft Material: Histological and Micro-Computed Tomography Assessment Using a Rat Calvarial Defect Model. 一种新型硅酸钙改性碳酸钙移植材料骨再生潜能的比较评价：用大鼠颅骨缺损模型进行组织学和显微计算机断层扫描评估。

IF 5.2 3区医学

Journal of Functional Biomaterials Pub Date : 2025-09-09 DOI: 10.3390/jfb16090337

Masataka Nakayama, Yu Kataoka, Naoki Kitamura, Chie Watanabe, Satoko Kujiraoka, Kikue Yamaguchi, Yuma Seki, Toshitake Furusawa, Hidero Unuma, Motohiro Munakata

{"title":"Comparative Evaluation of the Bone Regenerative Potential of a Novel Calcium Silicate-Modified Calcium Carbonate Graft Material: Histological and Micro-Computed Tomography Assessment Using a Rat Calvarial Defect Model.","authors":"Masataka Nakayama, Yu Kataoka, Naoki Kitamura, Chie Watanabe, Satoko Kujiraoka, Kikue Yamaguchi, Yuma Seki, Toshitake Furusawa, Hidero Unuma, Motohiro Munakata","doi":"10.3390/jfb16090337","DOIUrl":"10.3390/jfb16090337","url":null,"abstract":"In the present study, we evaluated the usefulness of a porous sintered calcium carbonate body with CaSiO3 by comparing its osteogenic capacity with that of calcium carbonate without CaSiO3 and that of β-tricalcium phosphate (TP). A cranial defect model of eight-week-old male Wistar rats was divided into three groups: calcium carbonate (CC), calcium carbonate-CaSiO3 composite (CC+CS), and TP. Micro-computed tomography (CT) and histological analysis were performed at four and eight weeks postoperatively. Upon quantitative evaluation of newly formed bone volume by radiography, the CC+CS group demonstrated the highest value at eight weeks postoperatively and exhibited significantly more new bone formations than the CC group (p < 0.05). Upon histological evaluation, the CC+CS group demonstrated significantly higher new bone formation than the CC group (p < 0.05). Furthermore, in terms of residual graft material ratio, at eight weeks postoperatively, the amount of residual graft material in the CC+CS group was significantly higher than that in the TP group (p < 0.05). Therefore, the addition of CaSiO3 enhances the functional regulation of calcium carbonate-based artificial bone and can be incorporated in bone graft materials.","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 9","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470458/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intraoperative Biologization of β-TCP and PCL-TCP by Autologous Proteins. 术中自体蛋白对β-TCP和PCL-TCP的生物学作用。

IF 5.2 3区医学

Journal of Functional Biomaterials Pub Date : 2025-09-09 DOI: 10.3390/jfb16090340

Andrea Sowislok, Gerrit Gruber, Farnusch Kaschani, Markus Kaiser, Eleftherios Papaeleftheriou, Marcus Jäger

{"title":"Intraoperative Biologization of β-TCP and PCL-TCP by Autologous Proteins.","authors":"Andrea Sowislok, Gerrit Gruber, Farnusch Kaschani, Markus Kaiser, Eleftherios Papaeleftheriou, Marcus Jäger","doi":"10.3390/jfb16090340","DOIUrl":"10.3390/jfb16090340","url":null,"abstract":"Protein adsorption on orthopedic biomaterials during the initial intraoperative contact critically influences biological responses and osseointegration. Osteoconductive grafts such as β-tricalcium phosphate (β-TCP) and poly (ε-caprolactone)-β-TCP (PCL-TCP) can be functionally activated by exposure to autologous tissue. However, the composition and relevance of the resulting protein layer still remain unclear. In this study involving 10 patients undergoing primary total hip arthroplasty, β-TCP and PCL-TCP samples were incubated both in the femoral medullary cavity and within a surgical tissue collector harvesting autologous tissue (blood, bone fragments, muscle, and fat). Surface morphology was assessed microscopically, and protein adsorption was characterized via high-resolution LC-MS/MS with subsequent bioinformatics and statistical analysis. Both materials adsorbed over 2000 different autologous proteins. β-TCP showed higher overall protein concentrations, while PCL-TCP demonstrated greater proteomic diversity and incubation method-dependent shifts in protein profiles, influenced by surface roughness and wettability. Samples incubated in the tissue collector exhibited less protein variability and smaller material-specific differences compared to incubation in the femoral cavity, particularly for PCL-TCP. Predominant proteins were linked to immune regulation, stress response, and protein metabolism. These findings emphasize the impact of material properties and incubation environment on protein adsorption, with ex vivo incubation leading to more consistent protein adsorption patterns.","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 9","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12470415/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Commercial and Experimental Fibre-Reinforced Composites in Restoring Endodontically Treated Teeth with Minimal Coronal Dentine: An In Vitro Study. 商业纤维增强复合材料与实验纤维增强复合材料修复牙髓治疗后冠状牙本质最小的牙体的比较：一项体外研究。

IF 5.2 3区医学

Journal of Functional Biomaterials Pub Date : 2025-09-08 DOI: 10.3390/jfb16090335

Amre R Atmeh, Faisal Masaud, Luba AlMuhaish, Abdulkarim Alanazi, Hadeel Almutiri, Saqib Ali, Hassan Almoqhawi, Abdul Samad Khan

{"title":"Comparison of Commercial and Experimental Fibre-Reinforced Composites in Restoring Endodontically Treated Teeth with Minimal Coronal Dentine: An In Vitro Study.","authors":"Amre R Atmeh, Faisal Masaud, Luba AlMuhaish, Abdulkarim Alanazi, Hadeel Almutiri, Saqib Ali, Hassan Almoqhawi, Abdul Samad Khan","doi":"10.3390/jfb16090335","DOIUrl":"10.3390/jfb16090335","url":null,"abstract":"Aim: To compare the fracture resistance of teeth with varying degrees of residual coronal dentine after restoration using two fibre-reinforced composite core materials.Materials and methods: Seventy extracted human lower premolars were divided into four groups: sound (control), one missing proximal wall (Cl-II), two missing proximal walls (MOD), and endocrown (EC). Subgroups were restored with either a short fibre-reinforced flowable composite (EverX Flow) or an experimental fibre-reinforced composite. Except for the control, teeth underwent endodontic treatment and were restored accordingly. Fracture resistance was tested using a universal testing machine. Statistical analysis compared fracture resistance across groups.Results: Teeth in EC exhibited the highest fracture resistance (1153.43 ± 332.52 N), comparable to sound teeth (1114.03 ± 185.58 N) and not significantly different from the experimental composite group (1006.89 ± 200.51 N) (p = 0.304). Cl-II restorations with EverX had significantly lower strength (652.48 ± 314.04 N) compared to MOD (773.02 ± 261.18 N) and EC (p < 0.05). The experimental composite showed a similar trend, with MOD having the lowest strength (408.6 ± 168.85 N). Significant differences were noted between materials in the MOD group (p = 0.009). Scanning electron microscopy revealed distinct fracture patterns.Conclusions: Endocrowns using direct fibre-reinforced composites provided protection for endodontically treated teeth with higher fracture resistance compared to teeth with MOD and Cl-II cavities. This gives direct composite endocrowns a potential for high-stress applications, though design and material selection remain critical.","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 9","pages":""},"PeriodicalIF":5.2,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12471155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0