Journal of Functional Biomaterials最新文献_第10页

Spatial Platform for Periodontal Ligament Angulation and Regeneration: In Vivo Pilot Study. 牙周韧带成角与再生的空间平台：体内先导研究。

IF 5 3区医学

Journal of Functional Biomaterials Pub Date : 2025-03-13 DOI: 10.3390/jfb16030099

Min Guk Kim, Do-Yeon Kim, Hyoung-Gon Ko, Jin-Seok Byun, Joong-Hyun Kim, Chan Ho Park

{"title":"Spatial Platform for Periodontal Ligament Angulation and Regeneration: In Vivo Pilot Study.","authors":"Min Guk Kim, Do-Yeon Kim, Hyoung-Gon Ko, Jin-Seok Byun, Joong-Hyun Kim, Chan Ho Park","doi":"10.3390/jfb16030099","DOIUrl":"10.3390/jfb16030099","url":null,"abstract":"The periodontal ligament (PDL) is a fibrous connective tissue that anchors the tooth-root surface to the alveolar bone with specific orientations. It plays a crucial role in functional restoration, optimal position stabilities, biomechanical stress transmission, and appropriate tissue remodeling in response to masticatory loading conditions. This pilot study explored spatial microarchitectures to promote PDL orientations while limiting mineralized tissue formation. A computer-designed perio-complex scaffold was developed with two parts: (1) PDL-guiding architectures with defined surface topography and (2) a bone region with open structures. After SEM analysis of micropatterned topographies on PDL-guiding architectures, perio-complex scaffolds were transplanted into two-wall periodontal defects in the canine mandible. Despite the limited bone formation at the 4-week timepoint, bone parameters in micro-CT quantifications showed statistically significant differences between the no-scaffold and perio-complex scaffold transplantation groups. Histological analyses demonstrated that the PDL-guiding architecture regulated fiber orientations and facilitated the functional restoration of PDL bundles in immunohistochemistry with periostin and decorin (DCN). The perio-complex scaffold exhibited predictable and controlled fibrous tissue alignment with specific angulations, ensuring spatial compartmentalization for PDL tissues and bone regenerations. These findings highlighted that the perio-complex scaffold could serve as an advanced therapeutic approach to contribute periodontal tissue regeneration and functional restoration in tooth-supporting structures.","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 3","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11942940/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Formulating Mechanically Robust Composite Restorative Materials for High Performance. 制定机械坚固的高性能复合修复材料。

IF 5 3区医学

Journal of Functional Biomaterials Pub Date : 2025-03-13 DOI: 10.3390/jfb16030101

Austyn Salazar, Natalie Anderson, Jeffrey Stansbury

{"title":"Formulating Mechanically Robust Composite Restorative Materials for High Performance.","authors":"Austyn Salazar, Natalie Anderson, Jeffrey Stansbury","doi":"10.3390/jfb16030101","DOIUrl":"10.3390/jfb16030101","url":null,"abstract":"Although dental resin composite restoratives offer a widely used direct-placement treatment option aimed at replacing the form and function of a natural tooth, there are several clinically relevant performance aspects of these materials that can be improved. The formulation of the resin matrix phase of dental composites for high-efficiency photopolymerization leading to polymers with excellent mechanical properties has always been a challenge that is addressed here through the use of structurally new and more reactive monomers as well as the formation of polymer networks that incorporate non-covalent reinforcing interactions. The purpose of this study was to validate that a set of tetraurethane diacrylates (TUDAs) with a novel configuration of their urethane linkages in coordination with acidic comonomers could be devised to obtain highly robust new composite materials. Due to the novel molecular design, this exploratory approach was conducted using reaction kinetics and three-point bend testing to assess the performance. Conversion and mechanical properties were measured to refine these formulations prior to the addition of filler. The initial formulations demonstrated outstanding dry mechanical test results that subsequently showed a major intolerance to water storage, which led to a model study using urethane diacrylate (UDA) followed by the addition of hydrophobic TUDA monomers. Once the resin formulations were optimized, silane-treated particulate filler was added to determine the effectiveness as composite materials. The final formulation used a hydrophobic, aromatic TUDA along with 4-methacryloxyethyl trimellitic anhydride (4-META) as a latent acidic comonomer and a mixture of acrylic acid (AA) and methacrylic acid (MAA). This formulation achieves a very high level of both reactivity and mechanical properties relative to current dental composite restoratives.","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 3","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11942826/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hybridization Chain Reaction-Enhanced Ultrasensitive Electrochemical Analysis of miRNAs with a Silver Nano-Reporter on a Gold Nanostructured Electrode Array. 在金纳米结构电极阵列上用银纳米报告细胞杂交链反应增强mirna的超灵敏电化学分析。

IF 5 3区医学

Journal of Functional Biomaterials Pub Date : 2025-03-12 DOI: 10.3390/jfb16030098

Bin Wang, Huiqiang Ma, Mingxing Zhou, Xian Huang, Ying Gan, Hong Yang

{"title":"Hybridization Chain Reaction-Enhanced Ultrasensitive Electrochemical Analysis of miRNAs with a Silver Nano-Reporter on a Gold Nanostructured Electrode Array.","authors":"Bin Wang, Huiqiang Ma, Mingxing Zhou, Xian Huang, Ying Gan, Hong Yang","doi":"10.3390/jfb16030098","DOIUrl":"10.3390/jfb16030098","url":null,"abstract":"Abnormal expression of miRNAs is associated with the occurrence and progression of cancer and other diseases, making miRNAs essential biomarkers for disease diagnosis and prognosis. However, the intrinsic properties of miRNAs, such as short length, low abundance, and high sequence homology, represent great challenges for fast and accurate miRNA detection in clinics. Herein, we developed a novel hybridization chain reaction (HCR)-based electrochemical miRNAs chip (e-miRchip), featured with gold nanostructured electrodes (GNEs) and silver nanoparticle reporters (AgNRs), for sensitive and multiplexed miRNA detection. AgNRs were synthesized and applied on the e-miRchip to generate strong redox signals in the presence of miRNA. The stem-loop capture probe was covalently immobilized on the GNEs, and was opened upon miRNA hybridization to consequently trigger the HCR for signal amplification. The multiple long-repeated DNA helix generated by HCR provides the binding sites for the AgNRs, contributing to the amplification of the electrochemical signals of miRNA hybridization. To optimize the detection sensitivity, GNEs with three distinct structures were electroplated, in which flower-like GNEs were found to be the best electrode morphology for miRNAs analysis. Under optimal conditions, the HCR-based e-miRchip showed an excellent detection performance with an LOD of 0.9 fM and a linear detection range from 1 fM to 10 pM. Moreover, this HCR-based e-miRchip platform was able to effectively distinguish miRNAs from the one- or two-base mismatches. This HCR-based e-miRchip holds great potential as a highly efficient and promising miRNA detection platform for the diagnosis and prognosis of cancer and other diseases in the future.","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 3","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943109/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing Flexor Digitorum Profundus Tendon Repair: A Narrative Review. 优化指深屈肌腱修复：叙述回顾。

IF 5 3区医学

Journal of Functional Biomaterials Pub Date : 2025-03-11 DOI: 10.3390/jfb16030097

Rishith R Mereddy, Emily E Zona, Camille J LaLiberte, Aaron M Dingle

{"title":"Optimizing Flexor Digitorum Profundus Tendon Repair: A Narrative Review.","authors":"Rishith R Mereddy, Emily E Zona, Camille J LaLiberte, Aaron M Dingle","doi":"10.3390/jfb16030097","DOIUrl":"10.3390/jfb16030097","url":null,"abstract":"Zone II flexor digitorum profundus (FDP) tendon injuries are complex, and present significant challenges in hand surgery, due to the need to balance strength and flexibility during repair. Traditional suture techniques often lead to complications such as adhesions or tendon rupture, prompting the exploration of novel strategies to improve outcomes. This review investigates the use of flexor digitorum superficialis (FDS) tendon autografts to reinforce FDP repairs, alongside the integration of biomaterials to enhance mechanical strength without sacrificing FDS tissue. Key biomaterials, including collagen-polycaprolactone (PCL) composites, are evaluated for their biocompatibility, mechanical integrity, and controlled degradation properties. Collagen-PCL emerges as a leading candidate, offering the potential to reduce adhesions and promote tendon healing. Although nanomaterials such as nanofibers and nanoparticles show promise in preventing adhesions and supporting cellular proliferation, their application remains limited by manufacturing challenges. By combining advanced repair techniques with biomaterials like collagen-PCL, this approach aims to improve surgical outcomes and minimize complications. Future research will focus on validating these findings in biological models, assessing tendon healing through imaging, and comparing the cost-effectiveness of biomaterial-enhanced repairs with traditional methods. This review underscores the potential for biomaterial-based approaches to transform FDP tendon repair.","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 3","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11942686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Guided Tissue Regeneration of Periodontal Infrabony Defects with Frozen Radiation-Sterilized Allogenic Bone Graft Versus Deproteinized Bovine Bone Mineral: 5-Year Outcomes of RCT. 冷冻辐射灭菌同种异体骨移植物与脱蛋白牛骨矿物质诱导牙周下颌骨缺损组织再生：5年RCT结果。

IF 5 3区医学

Journal of Functional Biomaterials Pub Date : 2025-03-10 DOI: 10.3390/jfb16030095

Bartłomiej Górski, Aniela Brodzikowska, Kacper Nijakowski, Mariano Sanz

{"title":"Guided Tissue Regeneration of Periodontal Infrabony Defects with Frozen Radiation-Sterilized Allogenic Bone Graft Versus Deproteinized Bovine Bone Mineral: 5-Year Outcomes of RCT.","authors":"Bartłomiej Górski, Aniela Brodzikowska, Kacper Nijakowski, Mariano Sanz","doi":"10.3390/jfb16030095","DOIUrl":"10.3390/jfb16030095","url":null,"abstract":"The aim of this study was to compare the efficacy of the guided tissue regeneration (GTR) of periodontal infrabony defects using the frozen radiation-sterilized allogenic bone graft (FRSABG) versus deproteinized bovine bone mineral (DBBM) 5 years after treatment. The association between patients' compliance and periodontitis recurrence with 5-year outcomes was also evaluated. Thirty infrabony defects in 15 stage III/IV periodontitis patients were randomly allocated to the FRSBAG group (tests) or the DBBM group (controls). Between 1 and 5 years, one patient was lost to follow-up and one tooth was extracted due to root fracture. No tooth was extracted for periodontal reasons. Consequently, 13 teeth in test sites and 14 teeth in control sites were available for the 5-year analysis. The clinical attachment level gain (CAL-G, primary outcome), probing pocket depth (PPD), radiographic defect depth (DD), and linear defect fill (LDF) were examined at baseline and 5 years post-surgically. Both groups showed statistically significant improvements in all evaluated clinical and radiographic parameters at 5 years, with insignificant intergroup differences. CAL-Gs were 4.46 ± 2.07 mm in the FRSBAG group, and 3.86 ± 1.88 mm in the DBBM group (p = 0.5442). In six (43%) patients, we observed periodontitis recurrence, among whom two (33.33%) participated regularly in supportive periodontal care (SPC) and the other four (66.7%) did not take part in SPC. A regression analysis revealed that periodontitis recurrence was a significant predictor of CAL loss and DD increase. FRSBAG and DBBM were both equally effective 5 years after the GTR of infrabony defects. Within the limitations of the present study, its outcomes advocate that both grafts may be considered as a viable option based on patient preferences and clinical considerations.","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 3","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11942688/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in the Properties of Different Zones in Multilayered Translucent Zirconia Used in Monolithic Restorations During Aging Process. 单层半透明氧化锆在老化过程中不同区域性能的变化

IF 5 3区医学

Journal of Functional Biomaterials Pub Date : 2025-03-10 DOI: 10.3390/jfb16030096

Phil-Joon Koo, Jong-Hyuk Lee, Seung-Ryong Ha, Deog-Gyu Seo, Jin-Soo Ahn, Yu-Sung Choi

{"title":"Changes in the Properties of Different Zones in Multilayered Translucent Zirconia Used in Monolithic Restorations During Aging Process.","authors":"Phil-Joon Koo, Jong-Hyuk Lee, Seung-Ryong Ha, Deog-Gyu Seo, Jin-Soo Ahn, Yu-Sung Choi","doi":"10.3390/jfb16030096","DOIUrl":"10.3390/jfb16030096","url":null,"abstract":"This study assessed the changes in the mechanical and surface properties of the transition zone in multilayered translucent monolithic zirconia subjected to long-term hydrothermal aging. A total of 360 disk-shaped specimens (diameter: 15.0 mm; thickness: 1.2 mm) were prepared using conventional (3Y-TZP in LT; ZL, 4Y-TZP in MT; ZM) and multilayered translucent zirconia (5Y-TZP in MT Multi; ZT, 3Y/5Y-TZP in Prime; ZP) among IPS e.max ZirCAD blocks. Specimens were divided into three groups (n = 30) and aged in the autoclave at 134 °C under 0.2 MPa for 0 h (control group), 5 h (first aged group), and 10 h (second aged group). The mechanical and surface properties of the transition zone in the multilayered translucent zirconia were investigated, followed by statistical analysis (α = 0.05). Before and after aging, ZL (1102.64 ± 41.37 MPa) and ZP (1014.71 ± 139.86 MPa) showed the highest biaxial flexural strength (BFS); ZL showed the highest Weibull modulus (31.46) and characteristic strength (1121.63 MPa); and ZT exhibited the highest nanoindentation hardness (20.40 ± 1.80 GPa) and Young's modulus (284.90 ± 20.07 GPa). After aging, ZL (116.75 ± 9.80 nm) exhibited the highest surface roughness (Ra); the monoclinic phase contents in ZL and ZP increased; and surface uplifts, microcracks, and irregular defects caused by phase transformation appeared on ZL and ZP surfaces. The 3Y/5Y-TZP transition zone exhibited flexural strength, Vickers hardness, phase distribution changes, and surface microstructure changes similar to those of 3Y-TZP before and after aging; however, the surface roughness was lower than that of 3Y-TZP and higher than those of 4Y-TZP and 5Y-TZP after aging. The mechanical and surface characteristics, excluding BFS and Vickers hardness, were influenced by the yttrium oxide content in each zone and the aging process.","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 3","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of ECM Components on Periodontal Ligament Stem Cell Differentiation Under Conditions of Disruption of Wnt and TGF-β Signaling Pathways. 在Wnt和TGF-β信号通路中断的情况下，ECM成分对牙周韧带干细胞分化的影响。

IF 5 3区医学

Journal of Functional Biomaterials Pub Date : 2025-03-09 DOI: 10.3390/jfb16030094

Alla V Kuznetsova, Olga P Popova, Tamara I Danilova, Andrey V Latyshev, Oleg O Yanushevich, Alexey A Ivanov

{"title":"Effects of ECM Components on Periodontal Ligament Stem Cell Differentiation Under Conditions of Disruption of Wnt and TGF-β Signaling Pathways.","authors":"Alla V Kuznetsova, Olga P Popova, Tamara I Danilova, Andrey V Latyshev, Oleg O Yanushevich, Alexey A Ivanov","doi":"10.3390/jfb16030094","DOIUrl":"10.3390/jfb16030094","url":null,"abstract":"Periodontitis is accompanied by inflammation that causes dysregulation of the Wnt/β-catenin and TGF-β signaling pathways. This leads to a violation of the homeostasis of periodontal tissues. Components of the extracellular matrix (ECM) are an important part of biomaterials used for the repair of periodontal tissue. The purpose of this study was to evaluate the components of the effect of ECM (hyaluronic acid (HA), fibronectin (Fn), and laminin (Lam)) on the osteogenic and odontogenic differentiation of periodontal ligament stem cells (PDLSCs) in the collagen I hydrogel under conditions of disruption of the Wnt/β-catenin and TGF-β signaling pathways. The study showed that the addition of components of the ECM restored the expression of odontogenic markers in PDLSCs, which was absent during inhibition of the canonical Wnt signaling pathway, and their multidirectional effect on the secretion of transforming growth factor-β1 (TGF-β1) and bone morphogenetic protein 2 (BMP-2). Fn and Lam suppressed the expression of odontogenic markers in PDLSCs against the background of inhibition of the TGF-β signaling pathway. The addition of HA under the conditions of the TGF-β signaling pathway improved BMP-2 secretion, preserving odontogenic differentiation. Thus, our results demonstrated that disruption of the Wnt/β-catenin and TGF-β signaling pathways causes disorders in the differentiation of PDLSCs, preventing the regeneration of periodontal tissues. This should be taken into account when developing multicomponent scaffolds that recapitulate the ECM microenvironment at endogenic regeneration of the periodontium. Inclusion of hyaluronic acid as one of these components may enhance the therapeutic effect of such biomaterials.","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 3","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11942902/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intraoperative Profiling of the Supracrestal Implant Complex Minimizes Peri-Implant Crestal Bone Remodeling: The Guided Bone Profiling Concept. 术中盖骨上种植体复合体的侧写减少种植体周围嵴骨重塑：引导骨侧写概念。

IF 5 3区医学

Journal of Functional Biomaterials Pub Date : 2025-03-08 DOI: 10.3390/jfb16030093

Milan Stoilov, Joerg Winterhoff, Lea Stoilov, Anastasia Timoschenko, Helmut Stark, Florian Heuser, Michael Marder, Dominik Kraus, Norbert Enkling

{"title":"Intraoperative Profiling of the Supracrestal Implant Complex Minimizes Peri-Implant Crestal Bone Remodeling: The Guided Bone Profiling Concept.","authors":"Milan Stoilov, Joerg Winterhoff, Lea Stoilov, Anastasia Timoschenko, Helmut Stark, Florian Heuser, Michael Marder, Dominik Kraus, Norbert Enkling","doi":"10.3390/jfb16030093","DOIUrl":"10.3390/jfb16030093","url":null,"abstract":"(1) Background: Early-stage bone resorption following implant placement can significantly impact the long-term success of implants. This study evaluates whether a fully digitally planned implant position based on the E-point concept, along with guided profiling of the supracrestal complex, contributes to improved stability of peri-implant bone levels. (2) Methods: 29 implants were placed in 27 patients utilizing both immediate (Group 1; n = 19) and delayed placement (Group 2; n = 10) protocols. Implant position and emergence profile were preoperatively determined and consistently executed through guided surgery and CAD/CAM-fabricated restorations. Due to the subcrestal positioning of the implant, a corresponding bone profiler with a guide pin was used to shape the emergence profile and prevent the provisional restoration from impinging on the proximal bone. Provisional restorations were immediately placed to support the emergence profile. Bone level changes were documented radiographically over a two-year period. The first Bone-to-Implant Contact Level (∆ fBIC), change in highest approximal Bone Level (∆ haBL), and formation of an emergence profile width (WEP) were measured. (3) Results: All implants and restorations survived after two years, no significant change in first Bone-to-Implant Contact Level (∆ fBIC = 0 ± 0.02 mm), no change in highest approximal Bone Level (∆ haBL) of -0.23 mm ± 0.71 mm, and formation of an emergence profile width (WEP) averaging 0.18 ± 0.19 mm. (4) Conclusions: Despite the initial stress on the bone caused by bone profiling, guided implant placement and bone shaping, supported by an immediate provisional, have a positive effect on peri-implant bone stability.","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 3","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11942669/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Antimicrobial Peptides (AMPs) in Treatment of Osteomyelitis in Human and Veterinary Orthopedics. 抗菌肽（AMPs）在人畜骨科骨髓炎治疗中的应用

IF 5 3区医学

Journal of Functional Biomaterials Pub Date : 2025-03-05 DOI: 10.3390/jfb16030090

Dominika Nguyen Ngoc, Michał Latalski, Anna Danielewicz, Tomasz Szponder, Joanna Wessely-Szponder, Ewa Mazur

{"title":"Application of Antimicrobial Peptides (AMPs) in Treatment of Osteomyelitis in Human and Veterinary Orthopedics.","authors":"Dominika Nguyen Ngoc, Michał Latalski, Anna Danielewicz, Tomasz Szponder, Joanna Wessely-Szponder, Ewa Mazur","doi":"10.3390/jfb16030090","DOIUrl":"10.3390/jfb16030090","url":null,"abstract":"Osteomyelitis, a severe bone infection, poses a significant therapeutic challenge in both human and veterinary medicine, especially due to the increasing prevalence of antibiotic-resistant pathogens like methicillin-resistant Staphylococcus aureus (MRSA). Conventional treatments, including surgical debridement and systemic antibiotics, often prove inadequate due to the ability of bacteria to form biofilms and evade host immune responses. Antimicrobial peptides (AMPs), such as LL-37 and β-defensins, have emerged as a promising alternative therapeutic strategy. AMPs exhibit broad-spectrum antimicrobial activity, including efficacy against resistant strains, and possess immunomodulatory properties that can promote bone regeneration. This article comprehensively reviews AMP applications in treating osteomyelitis across both human and veterinary medicine. We discuss diverse therapeutic approaches, including free AMPs, their conjugation with biomaterials such as collagen and chitosan to enhance delivery and stability, and the development of AMP-based nanoparticles. Furthermore, we analyze preclinical and clinical findings, highlighting the efficacy and safety of AMPs in combating osteomyelitis in both human and animal patients. Finally, we explore future perspectives and challenges, such as optimizing delivery, stability, and efficacy, while minimizing cytotoxicity, and in translating AMP-based therapies into clinical practice to effectively manage this debilitating disease.","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 3","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943317/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication and Characterization of Immature Porcine Cartilage-Derived Cell Biomembranes. 未成熟猪软骨细胞生物膜的制备与表征。

IF 5 3区医学

Journal of Functional Biomaterials Pub Date : 2025-03-05 DOI: 10.3390/jfb16030092

Phuong-Vy Bui, Vang Pham Thi, Trung-Nhan Vo, Viet-Trinh Nguyen, Thai-Duong Tran, Vy-Khanh Vo, Phuong Le Thi, Dieu Linh Tran, Minh-Dung Truong

{"title":"Fabrication and Characterization of Immature Porcine Cartilage-Derived Cell Biomembranes.","authors":"Phuong-Vy Bui, Vang Pham Thi, Trung-Nhan Vo, Viet-Trinh Nguyen, Thai-Duong Tran, Vy-Khanh Vo, Phuong Le Thi, Dieu Linh Tran, Minh-Dung Truong","doi":"10.3390/jfb16030092","DOIUrl":"10.3390/jfb16030092","url":null,"abstract":"(1) Background: Knee cartilage injury is at the top of the rising concerns among bone and joint disorder patients. Autologous chondrocyte implantation (ACI) is widely used to approach knee cartilage deterioration. Integrating autologous chondrocytes and periosteal patches aids in forming new cartilage-like tissue at the lesion area. This study uses a novel cell source from one-day-old porcine cartilage to fabricate a biomembrane as a substitute for periosteal membranes in cell implantation techniques for treating knee cartilage injuries. (2) Methods: Cells isolated from one-day-old porcine cartilage tissue were identified and assessed for their proliferation capability, differentiation ability, and membrane formation potential. The protein component of the biomembrane was also defined by proteomics. The cartilage repair ability was also confirmed using an in vitro transplantation model. (3) Results: Negative results for porcine infectious diseases are pivotal in selecting suitable piglets to provide cartilage tissue. The cells successfully obtained from one-day-old porcine cartilage exhibited stem-cell-like characteristics (CD34-, CD45-, CD90+, CD105+), including a high proliferation to 20 passages (doubling time: 1-2 days) and a capacity to differentiate into various cell types (osteogenesis, adipogenesis, and chondrogenesis). The stem cells were successfully applied in the fabrication of the biomembranes. The protein components of the biomembrane included an extracellular matrix and growth factors. The in vitro transplantation model showed that the biomembrane induced the repair ability of cartilage defects. (4) Conclusions: This study is the first to successfully harvest stem cells from one-day-old porcine cartilage for biomembrane fabrication for a knee cartilage injury therapeutic application.","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"16 3","pages":""},"PeriodicalIF":5.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11943366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143709965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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