Journal of dentistry最新文献

{"title":"Combined quantitative and relative microbiome profiling reveals key salivary bacteriome shifts associated with periodontal health and different stages of periodontitis","authors":"Junlin He ,&nbsp;Yefei Liu ,&nbsp;Haohao Liu ,&nbsp;Hui Liu ,&nbsp;Mi Xu ,&nbsp;Xiaoling Wei ,&nbsp;Meihua Chen","doi":"10.1016/j.jdent.2025.106125","DOIUrl":"10.1016/j.jdent.2025.106125","url":null,"abstract":"<div><h3>Aim</h3><div>To characterize the salivary microbiota in subjects with periodontal health (PH), stage I-II periodontitis (moderate periodontitis, MP), and stage III-IV periodontitis (severe periodontitis, SP) by integrating quantitative microbiome profiling (QMP) and relative microbiome profiling (RMP) approaches.</div></div><div><h3>Methodology</h3><div>This cross-sectional study involved the collection of 74 saliva samples, categorized into PH (n = 25), MP (n = 25), and SP (n = 24) groups, based on the 2018 Classification of Periodontal and Peri-Implant Diseases and Conditions. Samples were processed using absolute quantitative high-throughput sequencing technologies (Illumina NovaSeq). Alpha diversity, beta diversity, taxonomic shifts, bacterial types, and co-occurrence networks were compared across groups using QMP and RMP.</div></div><div><h3>Results</h3><div>Significant differences in microbial composition and microbial load were observed in the salivary microbiota across the PH, MP, and SP groups. With increasing periodontitis severity, salivary microbiota showed decreased alpha diversity but increased total abundance. Gram-negative anaerobic pathogens (e.g., <em>Porphyromonas, Prevotella, Treponema, Fusobacterium</em>) increased in both absolute and relative abundances. Conversely, aerobic and facultative anaerobic commensals (e.g., <em>Streptococcus, Haemophilus, Neisseria, Rothia</em>) showed increased absolute but decreased relative abundances. Additionally, co-occurrence networks constructed using QMP contained more species and connections compared to those constructed using RMP.</div></div><div><h3>Conclusion</h3><div>A strong association between increasing periodontitis severity and both elevated total microbial load and microbial dysbiosis, characterized by increased relative abundance of periodontal pathogens, was detected in the salivary microbiota. The combined QMP and RMP approach proved essential for capturing these complex microbial dynamics.</div></div><div><h3>Clinical Relevance</h3><div>Effective periodontitis management requires a dual approach: mechanical debridement to reduce microbial load and disrupt pathogenic ecological niches by decreasing pocket depth and inflammation, combined with targeted therapies to correct dysbiotic microbial communities and restore health-associated microbiota composition.</div></div>","PeriodicalId":15585,"journal":{"name":"Journal of dentistry","volume":"163 ","pages":"Article 106125"},"PeriodicalIF":5.5,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strategic approaches for enhancing the bioactivity of glass ionomer cement: A mechanistic and clinical perspective in terms of structural and surface modifications","authors":"Ali Saatchifard,&nbsp;Nader Nezafati,&nbsp;Saeed Hesaraki","doi":"10.1016/j.jdent.2025.106126","DOIUrl":"10.1016/j.jdent.2025.106126","url":null,"abstract":"<div><h3>Objectives</h3><div>To classify and evaluate current methods that increase the bioactivity of (GICs). This will highlight their clinical relevance and mechanism.</div></div><div><h3>Data/Sources</h3><div>Relevant literature was downloaded from PubMed and ScienceDirect, without consideration for language or publication year. Experiments that examined the surface, composition, and structural modification of GICs were included, both in vitro and in vivo studies.</div></div><div><h3>Study selection</h3><div>A critical analysis reviewed literature that focused on four main strategies: surface functionalization, liquid phase modification, powder phase modification, and additive addition. The review took into account ion release, apatite formation, antibacterial, and biological response based on reported findings. The results determined powder and liquid phase interventions to improve remineralization and bonding capacity. In contrast to this, bioactive additives like Bioactive Glass (BAG), chitosan, and nanoparticles increased ion exchange and antibacterial activity. While difficult to compare due to the difference in method and lack of long-term evidence, changes at the surface level improved cellular adhesion and apatite deposition.</div></div><div><h3>Conclusions</h3><div>Our review identified several laboratory methods that increase bioactivity. However, because studies differ in methods and report inconsistent mechanical results—and because long-term clinical data are scarce—it remains unclear which approaches will provide durable advantages for patients.</div></div><div><h3>Clinical Relevance</h3><div>Revised GICs are promising as restorative materials because they can integrate better with tissue, remineralize, and be resistant to bacteria better. However, when considering using them in the clinic, doctors should also discuss the possible benefits and the current uncertainties with patients. There is no normative evidence, and different modification methods suit different people.</div></div>","PeriodicalId":15585,"journal":{"name":"Journal of dentistry","volume":"163 ","pages":"Article 106126"},"PeriodicalIF":5.5,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validity and reliability of digital occlusal analyzing methods in Dentistry. A systematic review","authors":"Chahak Seth-Johansen,&nbsp;Klaus Gotfredsen","doi":"10.1016/j.jdent.2025.106124","DOIUrl":"10.1016/j.jdent.2025.106124","url":null,"abstract":"<div><h3>Objective</h3><div>This systematic review aimed to evaluate the validity and reliability of digital occlusal analyzing methods in terms of intraoral scanning (IOS) and sensor-based systems.</div></div><div><h3>Material and methods</h3><div>Following PRISMA guidelines, a comprehensive search was conducted across the databases: PubMed, Cochrane Library, Web of Science, Scopus and Embase. In vitro and in vivo studies published between January 1990 and July 2024, assessing the focused question: “Do digital occlusal analyzing methods provide sufficient reliability and validity in occlusal registration”. In vivo studies were limited to dentate or partially dentate patients. Exclusion criteria were systematic reviews, case reports, questionnaires, and studies not available in English.</div></div><div><h3>Results</h3><div>Thirty-nine studies were included (11 in vitro, 28 in vivo). Intraoral scanners (IOS) were assessed in 27 studies, while 15 studies investigated T-scan technology, 3 studies evaluated Dental Prescale, 2 studies explored the Accura system, and 2 studies with Occlusense®. Both IOS and the sensor-based systems demonstrated high degree of reliability in identifying occlusal contacts. However, validity in assessing contact intensity and exact interocclusal relationships was limited. Methodological heterogeneity between studies and lack of patient-reported outcomes were noted.</div></div><div><h3>Conclusions</h3><div>Digital methods, including both sensor-based systems and IOS, provide relatively reliable and valid illustration of teeth with interocclusal contacts, but show limited validity in evaluating contact intensity and area.</div></div><div><h3>Clinical implications</h3><div>Digital methods can indicate the location of tooth contacts, but their validity remains insufficient for making occlusal diagnoses used for occlusal corrections. These limitations suggest that digital occlusal tools are best used as adjuncts rather than replacements for traditional occlusal analysis. Future directions could be to include patient-reported outcomes and calibration guidelines for digital occlusal analysis systems.</div></div>","PeriodicalId":15585,"journal":{"name":"Journal of dentistry","volume":"163 ","pages":"Article 106124"},"PeriodicalIF":5.5,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145130856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface pretreatments for enhancing bond strength of resin composite to sclerotic dentin in non-carious cervical lesions: A systematic review","authors":"Kareem Hamdi ,&nbsp;Ahmed Zaeneldin ,&nbsp;Ahmed Hesham Samaha ,&nbsp;Hamdi H Hamama","doi":"10.1016/j.jdent.2025.106123","DOIUrl":"10.1016/j.jdent.2025.106123","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the impact of different surface pretreatments of cervical non-carious dentin on the bond strength to resin composite.</div></div><div><h3>Data sources</h3><div><em>In vitro</em> studies that assessed the impact of pretreatment of cervical non-carious dentin on bond strength to resin composite were considered for inclusion. The protocol was conducted following PRISMA guidelines and registered in PROSPERO (CRD420251102471). Studies were identified after comprehensive online search in 5 different electronic databases including PubMed (Medline), Web of science, Scopus, Cochrane library, and Google scholar.</div></div><div><h3>Results</h3><div>Eight studies met the inclusion criteria. However, the results were inconsistent, with some studies reporting no significant differences or even inferior outcomes with pretreatments like ethylenediaminetetraacetic acid (EDTA) or sodium hypochlorite. Prolonged application of phosphoric acid (30–40 %, 15–90 s) and combined chemical-mechanical pretreatments (<em>e.g.</em>, sandblasting with phosphoric acid) showed potential to improve bond strength compared to controls. Most studies exhibited a high risk of bias, primarily due to lack of blinding, inadequate sample size calculations, and failure to simulate clinical conditions.</div></div><div><h3>Conclusion</h3><div>Current scientific evidence suggests that pretreating cervical non-carious sclerotic dentin with prolonged application of 37 % phosphoric acid—either alone or in combination with mechanical roughening—may enhance bond strength to resin composite. However, the quality of the evidence is low. Further high-quality research, including studies on laser pretreatments and those simulating clinical conditions, is needed to establish reliable guidelines for clinical practice.</div></div><div><h3>Clinical significance</h3><div>Dentin of non-carious cervical lesion is physiologically different from normal dentin due to frequent and prolonged exposure to various oral conditions, such as thermal, chemical, and mechanical stimuli. Consequently, achieving reliable adhesion to this altered dentin substrate remains a clinical challenge. Therefore, surface pretreatment with prolonged application of 37 % phosphoric acid, either alone or in combination with mechanical roughening is recommended to increase the bond strength of resin composite to these lesions.</div></div>","PeriodicalId":15585,"journal":{"name":"Journal of dentistry","volume":"163 ","pages":"Article 106123"},"PeriodicalIF":5.5,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145130845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Social determinants of health are associated with molar-incisor hypomineralization (MIH): Results from a cross-sectional study in Bavaria, Germany","authors":"Nour Ammar ,&nbsp;Karl-Ferdinand Fresen ,&nbsp;Ramy Gaballah ,&nbsp;Helen Irina Schill ,&nbsp;Stefanie Amend ,&nbsp;Vinay Pitchika ,&nbsp;Joachim Heinrich ,&nbsp;Norbert Krämer ,&nbsp;Jan Kühnisch","doi":"10.1016/j.jdent.2025.106122","DOIUrl":"10.1016/j.jdent.2025.106122","url":null,"abstract":"<div><div>This population-based study investigated the associations between Molar Incisor Hypomineralization (MIH), caries, and the social determinants of health represented by a family’s socioeconomic status (SES), regional Gross Domestic Product (GDP), and regional population density in 8- to 10-year-olds from Bavaria, Germany.</div><div>A total of 5418 children from 87 randomly selected schools were clinically examined. Parental questionnaires were distributed to collect information on the families’ SES, and 1315 questionnaires (representing the subsample) were available. Regional GDP and population density data were retrieved from official databases. The data were analysed using marginalized zero-inflated negative binomial regression, and Incidence Rate Ratios (IRR) and adjusted Odds Ratios (aOR) were determined.</div><div>In the subsample (aged 9.7 ± 0.6 years, 47 % female), the prevalence rates of MIH and caries in the primary (idmft &gt; 0) and permanent dentitions (IDMFT &gt; 0) were 20.0 %, 33.0 %, and 14.6 %, respectively. Regression analyses revealed that the SES (IRR: 1.14 (1.02 – 1.27)), GDP (IRR: 2.58 (1.34 – 5.00); aOR: 5.23 (3.74 – 7.32)), and population density (IRR: 1.27 (1.11 – 1.45); aOR: 1.30 (1.14 – 1.48)) were significantly associated with MIH. Significant trends were observed between the SES (IRR: 0.90 (0.85 – 0.96); aOR: 0.90 (0.82 – 0.98)), GDP (IRR: 1.52 (1.77 – 1.96); aOR: 2.82 (1.80 – 4.42)), and population density (aOR: 1.13 (1.03 – 1.25)) and caries. Similar trends were observed in the data from the total sample.</div><div>The results revealed consistent positive associations between MIH and the social determinants of health. These findings challenge conventional assumptions about oral health disparities, as MIH was more prevalent in more prosperous, rather than disadvantaged populations.</div></div>","PeriodicalId":15585,"journal":{"name":"Journal of dentistry","volume":"163 ","pages":"Article 106122"},"PeriodicalIF":5.5,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a novel silver complex fluoride for caries management: an in vitro study","authors":"Grace Yuchan Xu ,&nbsp;Iris Xiaoxue Yin ,&nbsp;Irene Shuping Zhao ,&nbsp;Christie Ying Kei Lung ,&nbsp;Edward Chin Man Lo ,&nbsp;Chun Hung Chu","doi":"10.1016/j.jdent.2025.106121","DOIUrl":"10.1016/j.jdent.2025.106121","url":null,"abstract":"<div><h3>Objective</h3><div>The objective of this study is to develop a novel antibacterial and non-discolouring silver complex fluoride (SCF) for caries management.</div></div><div><h3>Methods</h3><div>SCF was synthesized by reacting 1,3,5-triaza-7-phosphaadamantane (PTA) with silver fluoride and characterised by electrospray ionization mass spectrometry. Silver and fluoride concentrations were measured by inductively coupled plasma atomic emission spectroscopy and fluoride ion-specific electrode. Alkalinity was measured using a pH meter. Cytotoxicity was assessed by determining the half maximal inhibitory concentration (IC₅₀) against the human gingival fibroblasts (HGF-1) and stem cells from human exfoliated deciduous teeth (SHED). Demineralised human dentine blocks were treated with SCF, incubated for 7 days with <em>Streptococcus mutans</em>, and assessed for antibacterial activity and discolouration. Biofilm kinetics and viability were analysed via colony-forming unit (CFU) counts and confocal laser scanning microscopy (CLSM). Dentine discolouration was quantified using colour spectrophotometry. Controls included 38% silver diamine fluoride (SDF; positive) and water (negative).</div></div><div><h3>Results</h3><div>At 180 days, 55% SCF solution contained 127,498 ± 3,535 ppm silver and 27,215 ± 118 ppm fluoride, with a pH of 10. SCF demonstrated higher IC₅₀ values compared to SDF in HGF-1 (35.5 ± 1.1 ppm vs. 17.7 ± 0.5 ppm; p &lt; 0.001) and SHED (95.5 ± 0.1 ppm vs. 6.4 ± 0.3 ppm; p &lt; 0.001). Log CFU counts for the SCF, SDF, and Water groups were 4.2 ± 0.2, 3.8 ± 0.4 and 5.2 ± 0.2 (SCF, SDF&lt;Water, p &lt; 0.001). CLSM showed dead-to-live ratios of SCF, SDF and Water group were 1.0 ± 0.3, 1.4 ± 0.5, and 0.2 ± 0.1 (p &lt; 0.001; SCF, SDF &gt; Water). Spectrophotometric analysis revealed ΔE values of SCF, SDF and Water groups were 5.8 ± 3.3, 73.0 ± 17.6, and 5.3 ± 2.7 (p &lt; 0.001; SCF, Water &lt; SDF).</div></div><div><h3>Conclusion</h3><div>A novel SCF solution with potent antibacterial efficacy and minimal discolouration was successfully developed.</div></div><div><h3>Clinical significance</h3><div>SCF demonstrates promising potential for clinical use in caries management, combining antimicrobial action with aesthetic preservation.</div></div>","PeriodicalId":15585,"journal":{"name":"Journal of dentistry","volume":"163 ","pages":"Article 106121"},"PeriodicalIF":5.5,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145124424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functionalization of titanium surfaces with bioactive and antimicrobial peptide coatings - A systematic review and meta-analysis","authors":"Lívia Maiumi Uehara ,&nbsp;Giovanna Lara de Souza Reis ,&nbsp;João Marcos Carvalho-Silva ,&nbsp;Andréa Cândido dos Reis","doi":"10.1016/j.jdent.2025.106116","DOIUrl":"10.1016/j.jdent.2025.106116","url":null,"abstract":"<div><h3>Background</h3><div>This systematic aimed to answer: \"How do peptide coatings functionalize titanium (Ti) surfaces?\". The question was structured according to the PICOS strategy, PRISMA guidelines were followed and the review was registered in the Open Science Framework.</div></div><div><h3>Methods</h3><div>A personalized search strategy was applied to Embase, Lilacs, PubMed/MEDLINE, Science Direct, Scopus, Web of Science Google Scholar and ProQuest databases. Included studies were <em>in vitro, in vivo</em>, or <em>ex vivo</em> experiments investigating the antimicrobial and osteogenic effects of peptide-functionalized Ti surfaces against <em>Sthaphylococcus aureus</em> and/or MC3T3-E1 cells. Excluded papers were clinical studies, reviews, studies lacking Ti surfaces or controls, those not using <em>S. aureus</em> or MC3T3-E1 cells, or involving non-murine models or peptide coatings combined with other agents. The risk of bias was assessed using RoBDEMAT for <em>in vitro</em> and Syrcle for <em>in vivo</em> studies. Meta-analysis was performed using inverse variance method under a random-effects model, 95 % confidence intervals (95 % CI), p-values, and heterogeneity index (I2).</div></div><div><h3>Results</h3><div>A total of 1731 potential studies were identified, 728 were screened by title and abstract, 52 were selected for full reading, 43 were included in this systematic review and all studies showed high risk of bias. <em>In vitro,</em> peptide-functionalized Ti surfaces significantly reduced <em>S. aureus</em> adhesion and proliferation (effect size: -8.10, 95 % CI [-13.33 to -2.86], <em>p</em> = 0.002) and showed slightly higher cytotoxicity (0.67 [0.37–0.98], <em>p</em> &lt; 0.001) for MC3T3-E1 cells, but enhanced bone mineralization (4.63 [2.44–6.81], <em>p</em> &lt; 0.001) and expression of pro-osteogenic genes (2.30 [1.89–2.71], <em>p</em> &lt; 0.001). <em>In vivo</em>, functionalized implants led to greater new bone formation (7.81 [5.44–10.18], <em>p</em> &lt; 0.001), trabecular number (11.22 [3.78–18.66], <em>p</em> = 0.003), and thickness (6.38 [3.05–9.71], <em>p</em> = 0.0002).</div></div><div><h3>Discussion</h3><div>Peptide coating of Ti surfaces for dental application can optimize implant performance, reduce infection and failure rates, and contribute to the development of safer and more effective therapies in oral rehabilitation. Therefore, this systematic review answered its question, as Ti coatings with peptides show antimicrobial and osteogenic activity as a surface functionalization mechanism.</div></div>","PeriodicalId":15585,"journal":{"name":"Journal of dentistry","volume":"163 ","pages":"Article 106116"},"PeriodicalIF":5.5,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145124422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of sagittal and transverse condylar inclination measurement techniques in virtual articulator programming: a technical report","authors":"Hong-lei Lin ,&nbsp;Zheng-xing Lin ,&nbsp;Ling-hui Jia ,&nbsp;Zhi-cen Lu ,&nbsp;Hao Yu","doi":"10.1016/j.jdent.2025.106120","DOIUrl":"10.1016/j.jdent.2025.106120","url":null,"abstract":"<div><h3>Objectives</h3><div>This technical evaluation aimed to compare 5 techniques for measuring sagittal (SCI) and transverse condylar inclination (TCI) to optimize virtual articulator programming in digital dentistry.</div></div><div><h3>Methods</h3><div>14 healthy participants (7 males, 7 females; aged 18–25 years) with 28 temporomandibular joints were evaluated. A novel virtual facebow system was developed to assess 5 measurement approaches based on natural head position (NHP) with horizontal plane markers: (1) Adjustable articulator (AAG); (2) Facial/intraoral scan integration (FIG); (3) CBCT/intraoral scan fusion (CIG); (4) Direct CBCT measurement (CTG), and (5) Kinematic facebow with T-Scan analysis (KFG). Triplicate measurements of interocclusal, protrusive, and lateral records were averaged. Statistical analysis included paired <em>t</em>-tests for bilateral comparisons and repeated measures ANOVA with Bonferroni-Greenhouse-Geisser corrections for technique comparisons.</div></div><div><h3>Results</h3><div>No significant differences were observed between left and right condylar inclination values (<em>P</em> &gt; 0.05). Significant differences were detected among the measurement techniques for both SCI and TCI values (<em>P</em> &lt; 0.001). Specifically, for SCI, KFG (41.81 ± 13.92°), CIG (43.05 ± 13.38°), and FIG (43.60 ± 12.64°) showed similar results (<em>P</em> &gt; 0.05), while CTG (47.52 ± 9.12°) produced significantly higher values than AAG (37.68 ± 9.75°) (<em>P</em> &lt; 0.05). For TCI, KFG (12.58 ± 7.27°), FIG (13.91 ± 7.72°), and CIG (11.85 ± 8.14°) again demonstrated similarity (<em>P</em> &gt; 0.05), whereas both CTG (18.57 ± 8.75°) and AAG (19.50 ± 6.33°) yielded significantly higher values (<em>P</em> &lt; 0.05).</div></div><div><h3>Conclusions</h3><div>Digital workflows based on natural head position with horizontal plane markers—integrating facial, intraoral, and cone beam computed tomography data—achieve sagittal and transverse condylar inclination values comparable to kinematic facebow recordings, supporting their use as reliable alternatives for virtual articulator programming.</div></div><div><h3>Clinical significance</h3><div>Integrated natural head position‑based digital protocols provide efficient, clinically valid solutions for condylar inclination assessment, supporting the transition to digital prosthodontic workflows.</div></div>","PeriodicalId":15585,"journal":{"name":"Journal of dentistry","volume":"163 ","pages":"Article 106120"},"PeriodicalIF":5.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145112887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of molar incisor hypomineralization-related enamel defects in anterior teeth on peer perception of smile esthetics in children and adolescents","authors":"Berkant Sezer ,&nbsp;Zeynep Sude Akbaş ,&nbsp;Asena Tuğçe Kara","doi":"10.1016/j.jdent.2025.106117","DOIUrl":"10.1016/j.jdent.2025.106117","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to evaluate the influence of enamel defects in anterior teeth associated with molar incisor hypomineralization (MIH) on peer perception of smile esthetics in children and adolescents.</div></div><div><h3>Methods</h3><div>A total of 288 children and adolescents aged 10–14 years from both public and private schools participated. Each participant evaluated standardized photographs of peers’ smiles, either showing MIH-related anterior enamel opacities or digitally altered to appear defect-free. Participants rated each image across four psychosocial domains: athletic ability, social competence, leadership potential, and academic performance. Independent-sample t-tests were used to compare ratings between smile types, and General Linear Models (GLMs) were applied to analyze the influence of evaluator-related variables such as age and gender.</div></div><div><h3>Results</h3><div>Digitally modified smiles received significantly higher ratings than unedited images for athletic ability (59.72 ± 0.86 vs. 50.94 ± 0.89; mean difference = 8.78, p &lt; 0.001), social competence (52.62 ± 0.80 vs. 43.78 ± 0.81; difference = 8.84, p &lt; 0.001), leadership potential (64.15 ± 0.84 vs. 56.44 ± 0.86; difference = 7.71, p &lt; 0.001), and academic performance (62.52 ± 0.79 vs. 58.36 ± 0.83; difference = 4.16, p &lt; 0.001). GLM analysis indicated that evaluator age and gender were stronger predictors than smile type, with older children and female evaluators demonstrating greater esthetic sensitivity (p &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>MIH-related enamel defects in anterior teeth negatively affect peer perception, but evaluator-related factors such as age and gender play an important role in shaping social judgments.</div></div><div><h3>Clinical significance</h3><div>This study demonstrates that enamel defects in anterior teeth caused by MIH not only present a clinical challenge but also influence how children are perceived by their peers in terms of social attributes.</div></div>","PeriodicalId":15585,"journal":{"name":"Journal of dentistry","volume":"163 ","pages":"Article 106117"},"PeriodicalIF":5.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oraltypes of the human oral bacteriome and mycobiome","authors":"Xu Wang ,&nbsp;Xinming Zhang ,&nbsp;Shuangshuang Wu ,&nbsp;Zhimin Yan","doi":"10.1016/j.jdent.2025.106119","DOIUrl":"10.1016/j.jdent.2025.106119","url":null,"abstract":"<div><h3>Objectives</h3><div>The inter‑individual variations in oral microbial composition remain largely unknown. This study aims to identify the bacterial and fungal compositional patterns of human oral microbiome.</div></div><div><h3>Methods</h3><div>A total of 389 bacterial samples from 5 cohorts and 118 fungal samples from 3 cohorts were collected. Partitioning around medoid (PAM) clustering method was used to cluster the samples of 16S and ITS amplification, respectively. Considering that the clusters identified in gut microbiome were previously named as “enterotypes”, the identified clusters in oral microbiome were called “oraltypes”.</div></div><div><h3>Results</h3><div>In the oral bacteriome, <em>Prevotella, Streptococcus</em> and <em>Neisseria</em> were the most abundant genera. Meanwhile, two bacterial “oraltypes” dominated by <em>Neisseria</em> and <em>Veillonella</em> were identified. In the oral mycobiome, <em>Candida</em> and <em>Malassezia</em> were the most abundant genera, which were also the dominant genera of the two identified fungal “oraltypes”. Further analysis showed that bacterial “oraltypes” were associated with the human age.</div></div><div><h3>Conclusions</h3><div>There are two bacterial and two fungal compositional patterns in human oral microbiome.</div></div><div><h3>Clinical significance</h3><div>This study identified the “oraltypes”, which can be used to depict the homogeneity and heterogeneity of oral microbiota among populations, as well as to analyze the susceptibility to certain diseases in different individuals.</div></div>","PeriodicalId":15585,"journal":{"name":"Journal of dentistry","volume":"163 ","pages":"Article 106119"},"PeriodicalIF":5.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}