一种治疗龋齿的新型复合氟化银的研制：体外研究。

IF 5.5 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of dentistry Pub Date : 2025-09-20 DOI:10.1016/j.jdent.2025.106121

Grace Yuchan Xu , Iris Xiaoxue Yin , Irene Shuping Zhao , Christie Ying Kei Lung , Edward Chin Man Lo , Chun Hung Chu

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引用次数: 0

摘要

目的：研制一种新型抗菌、不变色的复合氟化银（SCF），用于预防龋病。方法：以1,3,5-三氮杂-7-磷金刚烷（PTA）与氟化银反应合成SCF，并采用电喷雾质谱法对其进行表征。采用电感耦合等离子体原子发射光谱和氟离子特异性电极测定了银和氟离子浓度。用pH计测量碱度。通过测定对人牙龈成纤维细胞（HGF-1）和人脱落乳牙（SHED）干细胞的半数最大抑制浓度（IC50）来评估细胞毒性。用SCF处理脱矿的人牙本质块，与变形链球菌孵育7天，并评估抗菌活性和变色情况。通过菌落形成单位（CFU）计数和共聚焦激光扫描显微镜（CLSM）分析生物膜动力学和活力。用显色分光光度法定量测定牙本质变色。对照包括38%的氟化二胺银（SDF；阳性）和水（阴性）。结果：在180天，55% SCF溶液中含有127,498±3,535 ppm的银和27,215±118 ppm的氟，pH为10。SCF在HGF-1中的IC50值高于SDF（35.5±1.1 ppm比17.7±0.5 ppm, p < 0.001）和SHED（95.5±0.1 ppm比6.4±0.3 ppm, p < 0.001）。SCF、SDF和water组的Log CFU计数分别为4.2±0.2、3.8±0.4和5.2±0.2 （SCF、SDF water）。分光光度法分析SCF、SDF和water的ΔE值分别为5.8±3.3、73.0±17.6和5.3±2.7 （p < 0.001; SCF、water < SDF）。结论：成功研制了一种新型抗菌效果强、变色小的SCF溶液。临床意义：SCF在预防龋齿方面具有良好的临床应用潜力，结合抗菌作用和美观性。

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Development of a novel silver complex fluoride for caries management: an in vitro study

Objective

The objective of this study is to develop a novel antibacterial and non-discolouring silver complex fluoride (SCF) for caries management.

Methods

SCF was synthesized by reacting 1,3,5-triaza-7-phosphaadamantane (PTA) with silver fluoride and characterised by electrospray ionization mass spectrometry. Silver and fluoride concentrations were measured by inductively coupled plasma atomic emission spectroscopy and fluoride ion-specific electrode. Alkalinity was measured using a pH meter. Cytotoxicity was assessed by determining the half maximal inhibitory concentration (IC₅₀) against the human gingival fibroblasts (HGF-1) and stem cells from human exfoliated deciduous teeth (SHED). Demineralised human dentine blocks were treated with SCF, incubated for 7 days with Streptococcus mutans, and assessed for antibacterial activity and discolouration. Biofilm kinetics and viability were analysed via colony-forming unit (CFU) counts and confocal laser scanning microscopy (CLSM). Dentine discolouration was quantified using colour spectrophotometry. Controls included 38% silver diamine fluoride (SDF; positive) and water (negative).

Results

At 180 days, 55% SCF solution contained 127,498 ± 3,535 ppm silver and 27,215 ± 118 ppm fluoride, with a pH of 10. SCF demonstrated higher IC₅₀ values compared to SDF in HGF-1 (35.5 ± 1.1 ppm vs. 17.7 ± 0.5 ppm; p < 0.001) and SHED (95.5 ± 0.1 ppm vs. 6.4 ± 0.3 ppm; p < 0.001). Log CFU counts for the SCF, SDF, and Water groups were 4.2 ± 0.2, 3.8 ± 0.4 and 5.2 ± 0.2 (SCF, SDF<Water, p < 0.001). CLSM showed dead-to-live ratios of SCF, SDF and Water group were 1.0 ± 0.3, 1.4 ± 0.5, and 0.2 ± 0.1 (p < 0.001; SCF, SDF > Water). Spectrophotometric analysis revealed ΔE values of SCF, SDF and Water groups were 5.8 ± 3.3, 73.0 ± 17.6, and 5.3 ± 2.7 (p < 0.001; SCF, Water < SDF).

Conclusion

A novel SCF solution with potent antibacterial efficacy and minimal discolouration was successfully developed.

Clinical significance

SCF demonstrates promising potential for clinical use in caries management, combining antimicrobial action with aesthetic preservation.

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来源期刊

Journal of dentistry 医学-牙科与口腔外科

CiteScore

7.30

自引率

11.40%

发文量

349

审稿时长

35 days

期刊介绍： The Journal of Dentistry has an open access mirror journal The Journal of Dentistry: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal of Dentistry is the leading international dental journal within the field of Restorative Dentistry. Placing an emphasis on publishing novel and high-quality research papers, the Journal aims to influence the practice of dentistry at clinician, research, industry and policy-maker level on an international basis. Topics covered include the management of dental disease, periodontology, endodontology, operative dentistry, fixed and removable prosthodontics, dental biomaterials science, long-term clinical trials including epidemiology and oral health, technology transfer of new scientific instrumentation or procedures, as well as clinically relevant oral biology and translational research. The Journal of Dentistry will publish original scientific research papers including short communications. It is also interested in publishing review articles and leaders in themed areas which will be linked to new scientific research. Conference proceedings are also welcome and expressions of interest should be communicated to the Editor.