Journal of dentistry最新文献

{"title":"Decellularized matrix-hyaluronic acid-alginate hybrid hydrogels to enable a multi-layered full-thickness oral mucosa-on-a-chip","authors":"Toan V. Phan ,&nbsp;Thanaporn Pimpakan ,&nbsp;Pipob Suwanchaikasem ,&nbsp;Waranyoo Phoolcharoen ,&nbsp;Sawang Kesdangsakonwut ,&nbsp;Padet Tummaruk ,&nbsp;Supansa Yodmuang ,&nbsp;Risa Chaisuparat ,&nbsp;Oranart Matangkasombut ,&nbsp;Yeo-Jun Yoon ,&nbsp;Jae-Yol Lim ,&nbsp;João N. Ferreira","doi":"10.1016/j.jdent.2025.106115","DOIUrl":"10.1016/j.jdent.2025.106115","url":null,"abstract":"<div><h3>Objective</h3><div>Oral mucosa-on-a-chip (OMoC) devices mimic oral epithelium but require continuous nutrient support from underlying lamina propria. However, the common Matrigel or Basement membrane extract (BME) may not effectively support the oral mucosa (OM) native architecture. This study investigated whether a hybrid hydrogel composed of OM decellularized extracellular matrix (OM dECM), hyaluronic acid (HA), and alginate (Alg) surpasses Matrigel and BME for OMoC applications.</div></div><div><h3>Methods</h3><div>Porcine OM tissues were decellularized using 0.1–1 % sodium dodecyl sulfate (SDS), then freeze-dried, and pepsin-digested to produce OM dECM hydrogels (5, 10, and 20 mg/mL). DNA quantification, histological, biochemical and rheological assays, electron microscopy, and mass spectrometry were performed for dECM/hydrogels. Normal oral keratinocytes (NOK), gingival fibroblasts (HGF), and human umbilical vein endothelial cells (HUVEC) cultured in hydrogels were evaluated with proliferation, cytotoxicity, and immunocytochemistry (ICC) assays, and compared to Matrigel/BME. Hybrid hydrogels were used to tri-culture NOK, HGF, and HUVEC in a polydimethylsiloxane (PDMS)-based microfluidic chip for OM modeling.</div></div><div><h3>Results</h3><div>The 1 % SDS decellularization preserved greater ECM components. OM dECM hydrogels enhanced NOK, HGF, and HUVEC viability/proliferation, outperforming Matrigel/BME. Expression of OM-related markers (K14, Ki67, p63, Involucrin, E-cadherin, Von Willebrand factor) increased with hydrogels. Hybrid hydrogel of HA/Alg and 10 mg/mL dECM better supported NOK stratification while maintaining the native phenotype of HGF and HUVEC compared to Matrigel. Within our PDMS-based OMoC, the hybrid hydrogel facilitated the formation of a multi-layered microtissue construct with a stratified epithelial layer, a stromal compartment, and an endothelial network, better replicating the structural features of native OM as compared to other matrices and commercial microfluidic devices.</div></div><div><h3>Conclusions</h3><div>A 1 % SDS decellularization protocol effectively preserved OM ECM, enabling development of an injectable OM dECM hydrogel. The optimized dECM–HA/Alg hybrid hydrogel outperformed Matrigel in supporting oral microtissue stratification, and phenotype maintenance, making it a superior alternative for OMoC applications.</div></div><div><h3>Clinical Significance</h3><div>This study developed a pre-clinical hybrid hydrogel-based OMoC that can more accurately replicate the oral mucosa ECM and tissue architecture when compared to Matrigel/BME. By enhancing the ECM mimicry, the dECM–HA/Alg hybrid hydrogel supported the development of an optimized OMoC for cytotoxicity screening of oral drugs and potential disease modeling of oral mucosal conditions.</div></div>","PeriodicalId":15585,"journal":{"name":"Journal of dentistry","volume":"163 ","pages":"Article 106115"},"PeriodicalIF":5.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145112851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fatigue behaviour of fully crystallised glass-based CAD/CAM ceramics","authors":"Shujiang Chen ,&nbsp;Chun Yin Kam ,&nbsp;Yun Hong Lee ,&nbsp;Xuedong Bai ,&nbsp;Yanning Chen ,&nbsp;James Kit Hon Tsoi","doi":"10.1016/j.jdent.2025.106118","DOIUrl":"10.1016/j.jdent.2025.106118","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to investigate the fatigue behaviour of a novel fully crystallised lithium disilicate CAD/CAM ceramic in comparison to glass-based CAD/CAM materials.</div></div><div><h3>Methods</h3><div>The fully crystallised lithium disilicate (Initial LiSi Block; <strong>LISI</strong>), along with a fully sintered zirconia-containing lithium silicate (ZLS) (Celtra DUO; <strong>DUO</strong>) and a leucite-based glass ceramic (Empress CAD; <strong>EMP</strong>), were investigated. Flexural strength and flexural modulus (<em>n</em> = 15) were tested via three-point bending. After adhesively luting to dentine analogue abutments (30 % glass fibre reinforced polyamide-nylon 6,6; RPN), CAD/CAM-fabricated anatomic maxillary premolar crowns were subjected to wet cyclic step-stress loading until fracture (500 N initial load, 100 N step size, 100,000 cycles per step, 20 Hz frequency; <em>n</em> = 12). After testing the data normality and homogeneity of variance, statistical analysis was performed using one-way ANOVA, Kruskal-Wallis test, and median-rank Weibull analysis, with survival probability assessed by Mantel-Cox Log-Rank test (α = 0.05, 95 % CI).</div></div><div><h3>Results</h3><div>Significant difference in flexural strength was detected among three glass-based ceramics (<em>p</em> &lt; 0.001), while no significant difference was observed in flexural modulus (<em>p</em> = 0.630). No significant difference was found in the fatigue failure load (FFL) and number of cycles until failure (NCF) among groups (<em>p</em> = 0.106 and 0.061, respectively), except for the significantly higher NCF of LISI than EMP (<em>p</em> = 0.049). Despite similar survival probabilities (<em>p</em> = 0.165), LISI showed the highest FFL and structural reliability, and the lowest relative standard deviation (RSD).</div></div><div><h3>Significance</h3><div>Three chairside glass-based CAD/CAM materials presented satisfactory fatigue properties for single-unit restorations, with LISI showing higher reliability under fatigue loading.</div></div>","PeriodicalId":15585,"journal":{"name":"Journal of dentistry","volume":"163 ","pages":"Article 106118"},"PeriodicalIF":5.5,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145112959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AI-enhanced orthodontic treatment planning - A scoping review on Evidence-based clinical application with commercial software overview.","authors":"Flavia Preda, Nehal Elshazly, Reinhilde Jacobs","doi":"10.1016/j.jdent.2025.106112","DOIUrl":"https://doi.org/10.1016/j.jdent.2025.106112","url":null,"abstract":"<p><strong>Objective: </strong>This scoping review aimed to identify evidence-based research papers on AI-enhanced treatment planning tools for orthodontics. The clinical relevance and applicability of academically validated AI tools were examined and complemented by an assessment of commercially available software.</p><p><strong>Data sources: </strong>PubMed, Embase, Web of Science, and Cochrane were searched up to February 2025 for English-language studies on AI-based orthodontic treatment planning tools.</p><p><strong>Study selection: </strong>Included studies were validation, accuracy, evaluation research papers; were available in full text; published in English; and focused on digital orthodontic treatment planning.</p><p><strong>Results: </strong>Of 307 studies identified, 17 met inclusion criteria. The most focused on AI-driven decision-making for orthodontic extractions and design. Others explored automation for deep-bite planning and expansion in mixed dentition. Several studies evaluated large language models (LLMs) for answering orthodontic questions. Included were eight evaluation studies, three validation studies, two accuracy studies, and three comparative studies. AI methods included machine learning, deep learning, and LLMs, with reported accuracies from 72% to 95%. Seven commercial AI tools for orthodontic treatment planning were identified.</p><p><strong>Conclusions: </strong>The reviewed studies primarily addressed key treatment planning decisions or broader treatment recommendations. Academically validated tools typically rely on clinician-provided text inputs, whereas commercial AI solutions can process raw clinical data, such as intraoral scans. There is a mismatch between academically validated tools and commercially available systems, which generally lack published validation. This gap highlights the need for validation of commercial tools to ensure effective clinical integration.</p><p><strong>Clinical significance: </strong>AI-based tools for orthodontic treatment planning might enhance clinical efficiency and promote consistency in decision-making. Both academic and commercial solutions demonstrate significant potential as decision-support systems, reinforcing - rather than replacing - the expertise of orthodontic professionals.</p>","PeriodicalId":15585,"journal":{"name":"Journal of dentistry","volume":" ","pages":"106112"},"PeriodicalIF":5.5,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of digital technologies for occlusal analysis in dentate arches: A systematic review.","authors":"Gökçen Dinçer, Camila Nogueira Chamma-Wedemann, Isabella Neme Ribeiro Dos Reis, Emily Vivianne Freitas da Silva, Gülce Çakmak, Burak Yılmaz, Newton Sesma","doi":"10.1016/j.jdent.2025.106114","DOIUrl":"https://doi.org/10.1016/j.jdent.2025.106114","url":null,"abstract":"<p><strong>Background: </strong>Digital occlusal analysis systems are increasingly utilized to enhance the accuracy and reproducibility of occlusal assessments in dentistry by providing quantitative data on occlusal forces and contact sequences. The variability in the methodologies and outcomes across studies necessitates a systematic evaluation of these technologies.</p><p><strong>Objective: </strong>This systematic review evaluates the accuracy, reproducibility, and clinical applications of digital occlusal analysis systems in dentate arches.</p><p><strong>Methods: </strong>MEDLINE/PubMed, Scopus, Web of Science, and Embase databases were searched for articles published up to September 2024. Inclusion criteria encompassed in vitro and clinical studies comparing digital occlusal analysis systems, including T-Scan, OccluSense, Accura, and intraoral scanners.</p><p><strong>Results: </strong>A total of 3354 articles were identified, of which eight were included. Studies consistently demonstrated superior accuracy and reproducibility of T-Scan systems in dynamic occlusal force assessment. Cerec Omnicam provided reliable static occlusal evaluations, particularly in anterior regions, while OccluSense was an affordable alternative, although reproducibility across various operators and settings was limited. The integration of intraoral scanners with digital occlusal analyzers significantly enhanced diagnostic precision, allowing the simultaneous visualization of occlusal contacts, force distribution, and dynamic sequences. However, variability in the calibration methods and reporting metrics was noted across studies.</p><p><strong>Conclusions: </strong>Digital occlusal analysis systems, particularly T-Scan, deliver enhanced accuracy and reproducibility for occlusal assessment. Combining systems such as T-Scan with intraoral scanners (Trios or Medit I600) optimizes the clinical outcomes by integrating dynamic force analyses with precise static visualization. Future research should prioritize standardizing methodologies and conducting robust clinical trials to establish comprehensive clinical guidelines.</p><p><strong>Clinical significance: </strong>This systematic review provides clinicians with evidence-based guidance to select appropriate digital occlusal analysis technologies, which can enhance diagnostic accuracy and patient care.</p>","PeriodicalId":15585,"journal":{"name":"Journal of dentistry","volume":" ","pages":"106114"},"PeriodicalIF":5.5,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145091813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unifying intraoral scanner, computer-aided manufacturing, and final crown accuracy: A virtual-fit method for marginal gap evaluation","authors":"Daniel Borbola,&nbsp;Janos Vag","doi":"10.1016/j.jdent.2025.106113","DOIUrl":"10.1016/j.jdent.2025.106113","url":null,"abstract":"<div><h3>Objectives</h3><div>To validate a high-resolution virtual-fit method for evaluating marginal gap (MG) across CAD/CAM crown fabrication stages and to determine whether the Root Sum of Squares (RSS) method can reliably estimate total error from independent intraoral scanning (IOS) and manufacturing (CAM) steps.</div></div><div><h3>Methods</h3><div>A typodont model with a prepared maxillary first premolar was scanned 12 times using the EmeraldS IOS. Cement space settings from 30 to 140 µm (in 10 µm steps) were applied to CAD designs. Thirty-six hybrid-ceramic crowns were milled using only the 70, 100, and 140 µm settings. Final crowns and the reference model were scanned using both the MeditT710 lab scanner and the ATOSQ industrial scanner. A unified marginal gap assessment method was applied after non-penetrative alignment (virtual-fit) to measure MG at the IOS, CAM, and final (IOS+CAM) stages. The total error was estimated using the RSS formula. A sensitivity analysis was conducted to verify statistical power.</div></div><div><h3>Results</h3><div>MG was significantly overestimated when the MeditT710 scanner was used compared to ATOSQ, particularly in the IOS stage. The RSS estimate (52.0 ± 5.7 µm) slightly overestimated the final MG at 70 µm spacing by 4.7 µm (p &lt; 0.01), likely due to STL mesh artifacts. At 100 µm and 140 µm spacing, no significant difference was observed. The virtual-fit method demonstrated high statistical power, capable of detecting differences as small as 6 µm. At the final stage, MG at 140 µm spacing was 6 µm higher than at 100 µm (p &lt; 0.05).</div></div><div><h3>Conclusions</h3><div>The virtual-fit method provides consistent, high-resolution marginal fit measurement throughout the CAD/CAM workflow and supports cumulative error modeling via the RSS approach.</div></div><div><h3>Clinical significance</h3><div>RSS modeling enables clinicians and researchers to assess whether new IOS or CAM systems meet clinical accuracy thresholds without physically testing all device combinations. Cement spacing between 70–100 µm provides reasonable accuracy for chairside CAD/CAM crown fabrication.</div></div>","PeriodicalId":15585,"journal":{"name":"Journal of dentistry","volume":"163 ","pages":"Article 106113"},"PeriodicalIF":5.5,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145091876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel full digital positioning guide for autogenous bone grafting in the esthetic zone","authors":"Bassem Nabil Elfahl ,&nbsp;Tamer Mohamed Nasr Mostafa ,&nbsp;Dalia Ghalwash ,&nbsp;Ahmed Hamdy","doi":"10.1016/j.jdent.2025.106102","DOIUrl":"10.1016/j.jdent.2025.106102","url":null,"abstract":"<div><h3>Objectives</h3><div>This study introduces a novel fully digital workflow for fabricating a positioning guide for autogenous bone grafts in the esthetic maxillary zone. The technique was applied to patients presenting with missing, mobile, or fractured anterior maxillary teeth requiring replacement with dental implants and autogenous block grafting.</div></div><div><h3>Methods</h3><div>Cone-beam computed tomography (CBCT) and intraoral scans were obtained and merged for virtual planning. Both harvesting and positioning guides were designed using CAD software and fabricated via 3D printing. During surgery, the autogenous bone plate was harvested using the digital harvesting guide and stabilized at the recipient site with the positioning guide. Postoperative CBCT scans and superimpositions with preoperative models were performed to evaluate accuracy.</div></div><div><h3>Results</h3><div>The full digital guide (FDG) enabled accurate and predictable harvesting and positioning of the autogenous bone block. The margin of difference between the planned standard tesselletion language (STL) models and the postoperative Digital Imaging and Communication in Medicine (DICOM) outcomes ranged from 0.6 mm (axial plane) to 1.0 mm (sagittal plane), demonstrating high fidelity to the digital plan.</div></div><div><h3>Conclusions</h3><div>Within the limitation of this study, the FDG technique provided a predictable and precise approach for harvesting and positioning autogenous block grafts in the esthetic zone. This workflow facilitates the transfer of preoperative digital planning to the clinical setting, ensuring stable fixation and minimizing intraoperative variability. Future prospective randomized trials are required to confirm these findings.</div></div><div><h3>Clinical Relevance</h3><div>The FDG technique enhances the accuracy, safety, and predictability of autogenous bone grafting in the esthetic zone, potentially reducing surgical time, minimizing risk of complications, and improving functional and aesthetic outcomes.</div></div>","PeriodicalId":15585,"journal":{"name":"Journal of dentistry","volume":"163 ","pages":"Article 106102"},"PeriodicalIF":5.5,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of post-processing procedures on the dimensional accuracy and internal gap of additively manufactured definitive implant-supported single crowns: An in vitro study","authors":"Zhen Mao ,&nbsp;Shengxin Weng ,&nbsp;Almira Ada Diken Turksayar ,&nbsp;Elisabeth Prause ,&nbsp;Alessandro Casamenti ,&nbsp;Florian Beuer ,&nbsp;Franziska Schmidt","doi":"10.1016/j.jdent.2025.106111","DOIUrl":"10.1016/j.jdent.2025.106111","url":null,"abstract":"<div><h3>Objectives</h3><div>To evaluate the effects of post-processing protocols on the accuracy and internal fit of 3D-printed definitive implant-supported single crowns (ISCs).</div></div><div><h3>Methods</h3><div>An ISC was digitally designed based on a 3D-printed model with an incorporated one-piece zirconia implant. 120 definitive ISCs were fabricated by additive manufacturing (AM) using a resin-based material. The crowns were divided into 12 experimental groups, and each group was subjected to combinations of post-washing and post-polymerization conditions. Variations included washing solution (96 % isopropyl alcohol (IPA) or pure ethanol), washing equipment (ultrasonic bath or rotary washer), and photopolymerization conditions (air-contact, nitrogen-contact, or low-vacuum). Ten ISCs were fabricated by subtractive manufacturing (SM) as the control group. The root mean square values (RMS) between the design and acquired scan data, and internal gap dimensions were calculated. Statistics involved the Mann-Whitney U test and three-way ANOVA.</div></div><div><h3>Results</h3><div>Using a rotary washer with IPA led to significantly higher accuracy in external and overall regions. In contrast, post-curing did not significantly affect the RMS of ISCs regardless of the analyzed region. No significant differences in internal gap dimensions were found among the various post-processing strategies. SM crowns demonstrated significantly lower accuracy in the intaglio surfaces and smaller internal gaps than AM ones.</div></div><div><h3>Conclusions</h3><div>Post-processing strategies affected the printing accuracy of AM ISCs but had no significant influence on internal fit. SM ISCs exhibited smaller internal gaps despite lower dimensional accuracy of the intaglio surface, which may lead to early contact during crown seating, which requires more attention to intaglio adjustments.</div></div><div><h3>Significance</h3><div>AM can be recommended for producing ISCs, while post-processing protocols should be carefully considered.</div></div>","PeriodicalId":15585,"journal":{"name":"Journal of dentistry","volume":"163 ","pages":"Article 106111"},"PeriodicalIF":5.5,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occlusal accuracy of digitally manufactured removable dentures: a systematic review of in vitro studies","authors":"Xiaoxia Wang ,&nbsp;Andrew B. Cameron ,&nbsp;Nicholas C․K. Heng ,&nbsp;John M. Aarts ,&nbsp;Joanne J․E Choi","doi":"10.1016/j.jdent.2025.106110","DOIUrl":"10.1016/j.jdent.2025.106110","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the occlusal accuracy of digitally manufactured removable dentures compared to conventional fabrication, and to identify key influencing factors.</div></div><div><h3>Methods</h3><div>A comprehensive literature search of Ovid MEDLINE(R), Scopus, and Web of Science (All Databases), was conducted up to December 2024, supplemented by manual searches in reference lists and Google Scholar. Eligible studies were peer-reviewed, published in English, available in full text, and investigated the occlusal accuracy of digitally manufactured removable dentures in vitro. Studies were excluded if they lacked a method to assess the accuracy of denture teeth, tooth sockets, or occlusal surfaces. Risk of bias was evaluated with the Quality Assessment Tool For In Vitro Studies.</div></div><div><h3>Results</h3><div>Twenty-one studies met inclusion criteria. Digitally manufactured removable dentures, particularly subtractively manufactured dentures, demonstrated superior occlusal accuracy compared to conventional dentures. Additive manufacturing showed promising results but was influenced by layer thickness (100 µm outperformed 50 µm in DLP, whereas 50 µm was superior in SLA), build orientation (0° outperformed 90°, which outperformed 45°), and post-processing protocols (post-curing of dentures on casts). Optimal denture base-tooth offset values (0.05–0.25 mm, tooth-type dependent), tooth basal shapes (projections/dimples), and monolithic designs enhanced accuracy, while teeth positioning keys did not improve outcomes. Methodological heterogeneity, including variations in specimen shapes and measurement techniques, was noted.</div></div><div><h3>Conclusions</h3><div>Digital workflows, especially subtractive manufacturing, offer significant advantages in enhancing the occlusal accuracy of removable dentures. Key factors such as design schemes, manufacturing parameters, post-processing protocols, and bonding strategies critically influence outcomes. Standardized research methodologies are needed to facilitate cross-study comparisons. Future studies should explore the impact of palatal morphology, specimen pre-treatment, post-washing parameters, and the accuracy of digitally manufactured removable partial dentures.</div></div><div><h3>Clinical Significance</h3><div>Digital removable dentures have superior occlusal accuracy compared to conventionally made dentures. Standardized workflow and analysis method for occlusal accuracy need further study and development to provide clinically relevant information to dental practitioners.</div></div>","PeriodicalId":15585,"journal":{"name":"Journal of dentistry","volume":"162 ","pages":"Article 106110"},"PeriodicalIF":5.5,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary microbiota in children with and without type 1 diabetes mellitus: A one-year follow-up study","authors":"Neslihan Yilmaz ,&nbsp;Ulvi Kahraman Gürsoy ,&nbsp;Daniel Belstrøm ,&nbsp;Recep Polat ,&nbsp;Mervi Gürsoy","doi":"10.1016/j.jdent.2025.106109","DOIUrl":"10.1016/j.jdent.2025.106109","url":null,"abstract":"<div><h3>Objective</h3><div>Longitudinal data on the composition of salivary microorganisms in type 1 diabetes mellitus (T1DM) patients are lacking. This study aimed to characterize and compare the salivary microbiota of children with and without T1DM in a longitudinal approach. We hypothesized that the bacterial composition in saliva differs between healthy and T1DM children in a 1-year period.</div></div><div><h3>Methods</h3><div>Overall, 55 children (4–15 years old; 26 with T1DM, 29 healthy controls) completed the study. Oral examinations (plaque index, bleeding on probing, and Decayed, Missing, Filled Teeth index) and unstimulated saliva sampling were performed at baseline and after 1 year. Microbial composition was assessed via 16S rRNA gene sequencing (V1-V3 region) and referenced against the Human Oral Microbiome Database.</div></div><div><h3>Results</h3><div>Beta diversity analysis (Principal coordinate analysis (PCoA)) showed greater separation between groups at baseline than at follow-up. Linear discriminant analysis effect size identified that T1DM was associated with <em>Fusobacterium</em> species, whereas <em>Rothia</em> species associated with health. Alpha diversity indexes (Chao 1, Shannon and Simpson) showed no significant differences between the groups (<em>P</em> &gt; 0.05).</div></div><div><h3>Conclusion</h3><div>Our results demonstrated that the salivary microbiota of T1DM children is significantly distinct from healthy controls during 1-year of follow-up. Future studies are needed to reveal whether improved T1DM management benefits microbial composition.</div></div><div><h3>Clinical significance</h3><div>The microbial shift in diabetic children may contribute to increased susceptibility to oral diseases, highlighting the importance of preventive dental care in this population.</div></div>","PeriodicalId":15585,"journal":{"name":"Journal of dentistry","volume":"163 ","pages":"Article 106109"},"PeriodicalIF":5.5,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical performance of restorations in anterior teeth using composites with two levels of translucency: split-mouth randomized clinical trial","authors":"C.R.G. Torres,&nbsp;M.C. Mailart,&nbsp;R.F.A. Pinatti,&nbsp;D.F. da Silva,&nbsp;J.C. Moreira,&nbsp;T.C. Pereira,&nbsp;V. Ruano,&nbsp;M.A.R. de Holanda,&nbsp;P.C.A. Barros,&nbsp;R.P. Campos,&nbsp;T.P. Prado,&nbsp;A.B. Borges","doi":"10.1016/j.jdent.2025.106108","DOIUrl":"10.1016/j.jdent.2025.106108","url":null,"abstract":"<div><h3>Objectives</h3><div>This study evaluated the clinical performance of high- and medium-translucency resin composite restorations in anterior teeth using a randomized split-mouth clinical trial design.</div></div><div><h3>Material and methods</h3><div>Participants received two class III or class IV restorations using different resin composites (<em>n</em> = 30). Control restorations were performed using the medium-translucency enamel shade composite (GrandioSO, Voco), along with opaque dentin shades. The experimental restorations were performed using a high-translucency enamel shade composite (Amaris, Voco), also combined with opaque dentin shades. The materials were applied in increments up to 2 mm thick. A self-etching adhesive system (Futurabond U, Voco) was used in all restorations. Calibrated examiners evaluated the restorations using the FDI criteria at 7 days, 1 year, and 2 years. Data was analyzed with the Fisher’s Exact test (α=0.05).</div></div><div><h3>Results</h3><div>No significant differences between the tested materials were found for any of the FDI criteria. At 2-year recall, all restorations received acceptable scores for esthetic and biological parameters, and 96.67 % of restorations in both groups exhibited acceptable scores for functional properties. All the participants in the medium-translucency group were satisfied with aesthetics and function, while 96.76 % of participants in the high-translucency group reported complete satisfaction.</div></div><div><h3>Conclusions</h3><div>Both restorative materials exhibited good clinical performance for the aesthetic, functional, and biological properties analyzed with no differences between them after 24-month follow-up.</div></div><div><h3>Clinical significance</h3><div>Both medium- and high-translucency resin composites provided excellent esthetic outcomes with acceptable functional and biological properties for anterior Class III and IV restorations.</div></div><div><h3>Registration number</h3><div>RBR-6dy4mgj.</div></div>","PeriodicalId":15585,"journal":{"name":"Journal of dentistry","volume":"163 ","pages":"Article 106108"},"PeriodicalIF":5.5,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}