Salivary microbiota in children with and without type 1 diabetes mellitus: A one-year follow-up study

IF 5.5 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of dentistry Pub Date : 2025-09-13 DOI:10.1016/j.jdent.2025.106109

Neslihan Yilmaz , Ulvi Kahraman Gürsoy , Daniel Belstrøm , Recep Polat , Mervi Gürsoy

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引用次数: 0

Abstract

Objective

Longitudinal data on the composition of salivary microorganisms in type 1 diabetes mellitus (T1DM) patients are lacking. This study aimed to characterize and compare the salivary microbiota of children with and without T1DM in a longitudinal approach. We hypothesized that the bacterial composition in saliva differs between healthy and T1DM children in a 1-year period.

Methods

Overall, 55 children (4–15 years old; 26 with T1DM, 29 healthy controls) completed the study. Oral examinations (plaque index, bleeding on probing, and Decayed, Missing, Filled Teeth index) and unstimulated saliva sampling were performed at baseline and after 1 year. Microbial composition was assessed via 16S rRNA gene sequencing (V1-V3 region) and referenced against the Human Oral Microbiome Database.

Results

Beta diversity analysis (Principal coordinate analysis (PCoA)) showed greater separation between groups at baseline than at follow-up. Linear discriminant analysis effect size identified that T1DM was associated with Fusobacterium species, whereas Rothia species associated with health. Alpha diversity indexes (Chao 1, Shannon and Simpson) showed no significant differences between the groups (P > 0.05).

Conclusion

Our results demonstrated that the salivary microbiota of T1DM children is significantly distinct from healthy controls during 1-year of follow-up. Future studies are needed to reveal whether improved T1DM management benefits microbial composition.

Clinical significance

The microbial shift in diabetic children may contribute to increased susceptibility to oral diseases, highlighting the importance of preventive dental care in this population.

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患有和不患有1型糖尿病的儿童唾液微生物群：一项为期一年的随访研究。

目的：缺乏1型糖尿病（T1DM）患者唾液微生物组成的纵向数据。本研究旨在通过纵向方法表征和比较有和没有T1DM的儿童的唾液微生物群。我们假设健康儿童和T1DM儿童在1年内唾液中的细菌组成是不同的。方法：总共有55名儿童（4-15岁，26名T1DM患者，29名健康对照）完成了研究。在基线和1年后进行口腔检查（牙菌斑指数、探牙出血、蛀牙、缺牙、补牙指数）和非刺激唾液取样。通过16S rRNA基因测序（V1-V3区）评估微生物组成，并参照人类口腔微生物组数据库。结果：Beta多样性分析（主坐标分析（PCoA））显示基线时组间差异大于随访时组间差异。线性判别分析效应大小发现T1DM与梭杆菌属相关，而罗氏菌属与健康相关。α多样性指数Chao 1、Shannon和Simpson组间差异无统计学意义（P < 0.05）。结论：我们的研究结果表明，在1年的随访中，T1DM儿童的唾液微生物群与健康对照组明显不同。未来的研究需要揭示改善T1DM管理是否有益于微生物组成。临床意义：糖尿病儿童的微生物变化可能导致对口腔疾病的易感性增加，突出了这一人群预防性牙科保健的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of dentistry 医学-牙科与口腔外科

CiteScore

7.30

自引率

11.40%

发文量

349

审稿时长

35 days

期刊介绍： The Journal of Dentistry has an open access mirror journal The Journal of Dentistry: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal of Dentistry is the leading international dental journal within the field of Restorative Dentistry. Placing an emphasis on publishing novel and high-quality research papers, the Journal aims to influence the practice of dentistry at clinician, research, industry and policy-maker level on an international basis. Topics covered include the management of dental disease, periodontology, endodontology, operative dentistry, fixed and removable prosthodontics, dental biomaterials science, long-term clinical trials including epidemiology and oral health, technology transfer of new scientific instrumentation or procedures, as well as clinically relevant oral biology and translational research. The Journal of Dentistry will publish original scientific research papers including short communications. It is also interested in publishing review articles and leaders in themed areas which will be linked to new scientific research. Conference proceedings are also welcome and expressions of interest should be communicated to the Editor.