Journal of Clinical Biochemistry and Nutrition最新文献_第3页

Andrographolide derivative CX-10 alleviates ulcerative colitis by modulating autophagy and cellular stress. 穿心莲内酯衍生物CX-10通过调节自噬和细胞应激减轻溃疡性结肠炎。

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-07-01 Epub Date: 2025-03-15 DOI: 10.3164/jcbn.24-203

Zhengshi Chen, Yongheng He, Yi Hong, Feng Yu, Tianyun Gong, Xiaoxiao Tong

{"title":"Andrographolide derivative CX-10 alleviates ulcerative colitis by modulating autophagy and cellular stress.","authors":"Zhengshi Chen, Yongheng He, Yi Hong, Feng Yu, Tianyun Gong, Xiaoxiao Tong","doi":"10.3164/jcbn.24-203","DOIUrl":"10.3164/jcbn.24-203","url":null,"abstract":"Ulcerative colitis (UC) is a debilitating inflammatory bowel disease that poses significant challenges in clinical management. Despite existing therapies, many patients fail to achieve adequate symptom relief, underscoring the need to address the underlying mechanisms contributing to the pathogenesis of UC. Andrographis paniculata has been extensively studied in traditional Chinese medicine for its anti-inflammatory properties. This study aimed to evaluate the effects of CX-10, a derivative of andrographolide, on autophagy, oxidative stress, and inflammation in UC. Using dextran sulfate sodium (DSS)-induced mouse model of UC, our findings demonstrated that CX-10 treatment resulted in significant reductions in body weight loss, Disease Activity Index (DAI), and histopathological injury scores, characterized by decreased inflammatory cell infiltration and mucosal damage compared to DSS-treated controls. Quantitative real-time PCR (qRT-PCR) revealed a marked restoration of autophagy-related genes Becn1 and Atg5 in CX-10-treated colonic tissues. Western blot analysis further confirmed enhanced autophagic flux, evidenced by significant increases in the LC3-II/I ratio. CX-10 treatment also led to reduced endoplasmic reticulum (ER) stress, indicated by decreases in the transcript and protein levels of GRP78 and CHOP. Consistent with the in vivo findings, in vitro studies demonstrated that CX-10 effectively enhanced autophagy and reduced oxidative stress in lipopolysaccharides (LPS)-treated HT-29 colonic epithelial cells and RAW 264.7 macrophages. This was accompanied by a marked decrease in reactive oxygen species (ROS) levels, as determined by DCFDA assays. In conclusion, CX-10 exerts protective effects against DSS-induced UC through modulation of autophagy and oxidative stress pathways, suggesting its potential as a novel therapeutic agent for managing UC.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"77 1","pages":"45-54"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12326248/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

8-Prenylnaringenin suppresses obesity in high-fat diet-fed C57BL/6J mice via adiponectin secretion. 8- prenylnaringin通过脂联素分泌抑制高脂饮食C57BL/6J小鼠的肥胖。

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-07-01 Epub Date: 2025-02-22 DOI: 10.3164/jcbn.24-214

Fukiko Okada, Akiko Kohara, Yuichi Ukawa, Rie Mukai, Hitoshi Ashida, Yoko Yamashita

{"title":"8-Prenylnaringenin suppresses obesity in high-fat diet-fed C57BL/6J mice via adiponectin secretion.","authors":"Fukiko Okada, Akiko Kohara, Yuichi Ukawa, Rie Mukai, Hitoshi Ashida, Yoko Yamashita","doi":"10.3164/jcbn.24-214","DOIUrl":"10.3164/jcbn.24-214","url":null,"abstract":"8-Prenylnaringenin (8-PN) is a prenylflavonoid found in hops (Humulus lupulus L.). It has several beneficial functions, which include the inhibition of bone loss and muscle atrophy. 8-PN is a metabolite of xanthohumol, which can prevent obesity in mice; however, the effect of 8-PN on obesity is still unknown. In the present study, we found that 8-PN prevented obesity in high-fat diet-fed mice. When C57BL6/J male mice were fed 8-PN at 0.0005% or 0.005% with a high-fat diet for 8 weeks, body weight gain, fat accumulation in adipose tissue, and fatty liver induced by the high-fat diet were prevented. In mice fed a high-fat diet and 8-PN, adenosine monophosphate-activated protein kinase (AMPK) was activated in visceral adipose tissue, which was accompanied by decreased expression of a fatty acid synthesis-related factor and increased expression of a mitochondrial biosynthesis-related factor downstream of AMPK. AMPK appeared to be activated by adiponectin secretion, which was associated with increased expression of adipocyte differentiation markers in mice fed a high-fat diet and 8-PN. For the first time, this study shows that 8-PN can prevent obesity in mice and that it is effective at low concentrations that humans could consume in their daily diet.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"77 1","pages":"64-73"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12326250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FOXM1 transcriptionally activates NEIL3 to inhibit ferroptosis in lung adenocarcinoma cells. FOXM1转录激活NEIL3抑制肺腺癌细胞铁下垂。

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-07-01 Epub Date: 2025-03-08 DOI: 10.3164/jcbn.24-136

Hailang Hou, Xinpu Geng, Xingxing Shao, Jindao Wang, Wan Xia, Huijie Chen

{"title":"FOXM1 transcriptionally activates NEIL3 to inhibit ferroptosis in lung adenocarcinoma cells.","authors":"Hailang Hou, Xinpu Geng, Xingxing Shao, Jindao Wang, Wan Xia, Huijie Chen","doi":"10.3164/jcbn.24-136","DOIUrl":"10.3164/jcbn.24-136","url":null,"abstract":"Lung adenocarcinoma (LUAD) is responsible for a substantial portion of cancer-related deaths, carrying a bleak treatment outlook. The application of ferroptosis-focused treatments has shown great potential. This research is committed to uncovering the molecular mechanisms by which Nei Like DNA Glycosylase 3 (NEIL3) impacts ferroptosis in LUAD, in the quest for robust biomarkers. Using The Cancer Genome Atlas database, qRT-PCR, and Western blot (WB), we evaluated the expression of NEIL3 in LUAD tissues and cells, then performed gene set enrichment analysis to identify enriched gene sets. Predictive tools hTFtarget and MoLoTool assisted in identifying potential upstream transcription factors and their promoter binding sites for NEIL3, following which we conducted Pearson correlation analysis. The binding affinity of NEIL3 to Forkhead box protein M1 (FOXM1) was validated with dual-luciferase and chromatin immunoprecipitation assays. Cell viability was determined by measuring MDA and Fe2+ content in cells with the aid of cell counting kit-8. Lipid reactive oxygen species (ROS) levels were detected by flow cytometry, and WB was employed to evaluate the expression of GPX4 and SLC7A11 proteins. An upregulation of NEIL3 is observed in LUAD tissues and cell lines, particularly within pathways linked to ferroptosis. When NEIL3 was knocked down, there was a decline in the viability of LUAD cells, coupled with elevated MDA, Fe2+, and lipid ROS levels. Protein expression of GPX4 and SLC7A11 was inhibited, but these phenotypes were rescued by the application of a ferroptosis inhibitor. FOXM1 could interact with the NEIL3 gene promoter, initiating its transcription. In the context of LUAD, the activation of NEIL3 by FOXM1 constitutes the FOXM1/NEIL3 axis that counteracts ferroptosis in LUAD cells.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"77 1","pages":"10-17"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12326245/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enteral nutrition intervention improves intestinal ischemia-reperfusion injury by modulating the SENP1/SIRT3 axis. 肠内营养干预通过调节SENP1/SIRT3轴改善肠缺血再灌注损伤。

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-07-01 Epub Date: 2025-04-09 DOI: 10.3164/jcbn.25-31

Shizhuang Wei, Zhenhua Li, Bo Wen, Wei Wang, Daolai Huang, Chao Zhang, Xianghua Wu

{"title":"Enteral nutrition intervention improves intestinal ischemia-reperfusion injury by modulating the SENP1/SIRT3 axis.","authors":"Shizhuang Wei, Zhenhua Li, Bo Wen, Wei Wang, Daolai Huang, Chao Zhang, Xianghua Wu","doi":"10.3164/jcbn.25-31","DOIUrl":"10.3164/jcbn.25-31","url":null,"abstract":"Intestinal ischemia-reperfusion (II/R) injury is a significant clinical concern with high mortality rates. Mitochondria play a crucial role in this process, and maintaining mitochondrial homeostasis is a potential treatment target. SENP1 is a de-SUMOylated hydrolase that may regulate SIRT3, a major mitochondrial deacetylase. However, the role of SENP1 and SIRT3 in II/R remains unclear. Employing a combination of in vitro cell culture experiments utilizing Caco-2 cells and in vivo II/R models with SD rats, along with an array of molecular biology techniques such as gene silencing, protein detection methods, immunoprecipitation, histological analysis, and functional assays, this study delved into the role of SENP1 and SIRT3 in intestinal ischemia-reperfusion injury. Statistical analysis was meticulously conducted to evaluate the significance of the obtained results. SENP1 and SIRT3 are co-expressed and interact in Caco-2 cells. In models of II/R, the expression of SENP1 increased while that of SIRT3 decreased. Reducing SENP1 expression by siRNA or enteral nutrition intervention with bupropion alleviated intestinal II/R injury, reduced mitochondrial damage and oxidative stress, and improved the number and function of mitochondria. Our study demonstrates the importance of SENP1 and SIRT3 in intestinal ischemia-reperfusion injury. Reducing SENP1 expression through siRNA or enteral nutrition intervention shows promise as a potential therapeutic approach. This research provides new insights into the mechanism of II/R injury and paves the way for further investigations.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"77 1","pages":"55-63"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12326244/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Levosimendan alleviates myocardial ischemia-reperfusion injury by regulating mitochondrial autophagy through cGAS-STING signaling pathway. 左西孟旦通过cGAS-STING信号通路调节线粒体自噬，减轻心肌缺血再灌注损伤。

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-07-01 Epub Date: 2025-03-18 DOI: 10.3164/jcbn.25-8

Feng Shi, Han Bao, Fanqing Meng, Yunting Pang

{"title":"Levosimendan alleviates myocardial ischemia-reperfusion injury by regulating mitochondrial autophagy through cGAS-STING signaling pathway.","authors":"Feng Shi, Han Bao, Fanqing Meng, Yunting Pang","doi":"10.3164/jcbn.25-8","DOIUrl":"10.3164/jcbn.25-8","url":null,"abstract":"Levosimendan, a calcium sensitizer, has cardioprotective effects against myocardial ischemia-reperfusion injury (MIRI). Mitophagy plays an important role in MIRI, and the cGAS-STING signaling pathway can participate in mitophagy in a variety of ways. The purpose of this study was to explore the new molecular mechanism by which levosimendan exerts cardioprotective effects in order to provide a new experimental basis for the clinical application of levosimendan. In this study, an isolated MIRI rat model was established, and 48 rats were randomly divided into four groups (n = 12): continuous perfusion group (Group C), ischemia-reperfusion group (IR group), ischemia-reperfusion + levosimendan group (IR + L group), and ischemia-reperfusion + levosimendan + sting activator group (IR + LA group). The hemodynamic indices, myocardial infarction volume, expression of cGAS-STING signaling pathway proteins, and mitophagy-related proteins in isolated rat hearts of the four groups were compared. This study showed that levosimendan can reduce the level of myocardial mitophagy in ischemia-reperfusion rats by inhibiting the cGAS-STING signaling pathway, reducing myocardial injury, and playing a myocardial protective role.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"77 1","pages":"74-78"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12326253/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of miRNAs associated with eosinophilic esophagitis shown by esophageal mucosal biopsy results of Japanese patients. 日本患者食管粘膜活检结果显示与嗜酸性粒细胞性食管炎相关的mirna鉴定。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-05-01 Epub Date: 2024-12-28 DOI: 10.3164/jcbn.24-193

Naoki Oshima, Norihisa Ishimura, Satoshi Kotani, Tomotaka Yazaki, Akihiko Oka, Nobuhiko Fukuba, Yoshiyuki Mishima, Hiroshi Tobita, Kotaro Shibagaki, Kousaku Kawashima, Yoshikazu Kinoshita, Shunji Ishihara

{"title":"Identification of miRNAs associated with eosinophilic esophagitis shown by esophageal mucosal biopsy results of Japanese patients.","authors":"Naoki Oshima, Norihisa Ishimura, Satoshi Kotani, Tomotaka Yazaki, Akihiko Oka, Nobuhiko Fukuba, Yoshiyuki Mishima, Hiroshi Tobita, Kotaro Shibagaki, Kousaku Kawashima, Yoshikazu Kinoshita, Shunji Ishihara","doi":"10.3164/jcbn.24-193","DOIUrl":"10.3164/jcbn.24-193","url":null,"abstract":"The number of eosinophilic esophagitis (EoE) patients has been rapidly increasing worldwide in recent years, though remains relatively lower in Japan. To determine whether Japanese EoE cases have pathogenic differences, microRNA (miRNA) transcriptome analyses of esophageal biopsy specimens from patients with EoE were performed to identify involved miRNAs and the results were compared with those for cases reported in Western countries. Japanese patients with EoE (n = 9) or reflux esophagitis (RE) (n = 4) were enrolled, with the latter serving as controls. Biopsies of esophageal mucosal tissue were performed as a part of an upper gastrointestinal endoscopy, followed by comprehensive miRNA expression analysis (2,588 target human miRNAs) using microarray technology. The EoE patients were then treated with a proton pomp inhibitor, and miRNA expression in the esophagus was compared before and after treatment. Hierarchical cluster analysis findings showed clear differences in miRNA expression patterns between the EoE and RE patients, with increased expression of 13 and decreased expression of 10 miRNAs noted in the EoE cases. These results were then compared with miRNA expression in esophageal mucosa of EoE patients presented in Western reports and the expression profiles were found to be very similar. In addition, changes in expression profiles of several miRNAs before and after treatment were observed in the present EoE patients. miRNA microarray analysis of Japanese EoE patients demonstrated a significant overlap of miRNA expression in comparison with Western patients, indicating that EoE likely represents the same disease among diverse racial populations and shows a consistent pathogenesis worldwide.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 3","pages":"289-295"},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152238/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary combination of sucrose and linoleic acid increases intramyocellular lipid and impairs muscle strength in female Zucker diabetic fatty rats. 膳食中蔗糖和亚油酸的组合增加了雌性Zucker糖尿病脂肪大鼠的细胞内脂质并损害了肌肉力量。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-05-01 Epub Date: 2025-01-18 DOI: 10.3164/jcbn.24-229

Masaki Takikawa, Hirokazu Ohminami, Takayuki Uchida, Ayano Kitamura, Yuichiro Adachi, Yuki Mori, Kohta Ohnishi, Masashi Masuda, Hisami Yamanaka-Okumura, Yutaka Taketani

{"title":"Dietary combination of sucrose and linoleic acid increases intramyocellular lipid and impairs muscle strength in female Zucker diabetic fatty rats.","authors":"Masaki Takikawa, Hirokazu Ohminami, Takayuki Uchida, Ayano Kitamura, Yuichiro Adachi, Yuki Mori, Kohta Ohnishi, Masashi Masuda, Hisami Yamanaka-Okumura, Yutaka Taketani","doi":"10.3164/jcbn.24-229","DOIUrl":"10.3164/jcbn.24-229","url":null,"abstract":"Sarcopenic obesity is associated with metabolic disorders and physical limitations. Intramyocellular lipid (IMCL) accumulation is a critical factor affecting muscle strength, independent of muscle mass, in obesity. While diet plays an important role in regulating IMCL, the effects of specific dietary combinations remain poorly understood. This study examined the effects of different combinations of dietary carbohydrates and fats on skeletal muscle quality and function in obese rats. Female Zucker diabetic fatty rats were fed diets containing either sucrose or palatinose combined with either oleic or linoleic acid for 12 weeks. Body and muscle weights, IMCL content, and grip strength were measured. C2C12 myotubes were treated in vitro with varying concentrations of glucose or insulin, along with fatty acids (oleic acid or linoleic acid), to mimic the exposure of each diet. The diet combining sucrose and linoleic acid (SL) significantly increased IMCL accumulation in the extensor digitorum longus (EDL) muscle and reduced grip strength. A negative correlation was observed between IMCL and grip strength. Cluster of differentiation 36 (CD36) protein levels tended to increase in the EDL of the SL diet-fed group. In vitro experiments demonstrated that high glucose levels combined with linoleic acid increased IMCL and CD36 expression. In conclusion, diets high in sucrose and linoleic acid exacerbate IMCL accumulation and reduce muscle strength in obese rats through hyperglycemia-induced interaction.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 3","pages":"271-279"},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152242/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum. 勘误表。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-05-01 DOI: 10.3164/jcbn.24-178_Erratum

引用次数: 0

Partially hydrolyzed guar gum ingestion suppresses atopic dermatitis-like symptoms through prebiotic effect in mice. 部分水解瓜尔胶摄入通过益生元效应抑制小鼠特应性皮炎样症状。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-05-01 Epub Date: 2025-02-06 DOI: 10.3164/jcbn.24-219

So Morishima, Aya Abe, Saki Okamoto, Mahendra P Kapoor, Shiho Matsuura, Kenji Kuriya, Makoto Ozeki, Masahiro Nishio, Hiroto Miura, Ryo Inoue

{"title":"Partially hydrolyzed guar gum ingestion suppresses atopic dermatitis-like symptoms through prebiotic effect in mice.","authors":"So Morishima, Aya Abe, Saki Okamoto, Mahendra P Kapoor, Shiho Matsuura, Kenji Kuriya, Makoto Ozeki, Masahiro Nishio, Hiroto Miura, Ryo Inoue","doi":"10.3164/jcbn.24-219","DOIUrl":"10.3164/jcbn.24-219","url":null,"abstract":"Growing knowledge reveals the association between the gut microbiome and skin, rendering the gut microbiome an appealing potential therapeutic target for atopic dermatitis (AD). In this study, we assessed the effect of partially hydrolyzed guar gum (PHGG) on AD-like symptoms induced by topical 1-Chloro-2,4-dinitrobenzene (DNCB) in BALB/c mice. Four weeks of PHGG feeding prevented the loss of epidermal barrier integrity and epithelial hyperplasia in the AD lesion (p<0.05, effect size >0.80), indicating a reduction in AD-like symptoms. According to the postulated mechanism, PHGG ingestion modulates the gut microbiome resulting in enhanced butyrate production (p<0.05). Butyrate suppresses Th2 function in gut immunity, which is believed to have significance in systemic immune regulation. The lowering of blood Th2 cytokines (IL-4 and IL-10, p<0.05) in the PHGG-fed group confirmed the existence of such a pathway, and butyrate can possibly be considered to have an indirect involvement in the suppression of Th2 immune response in the AD lesions. These findings encourage support for an association between gut microbiome and skin through the immune system, implying that daily PHGG ingestion may be beneficial for suppressing AD symptoms across the gut-immune-skin axis.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 3","pages":"280-288"},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152237/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of serum zinc level in patients with severe trauma. 严重创伤患者血清锌水平分析。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-05-01 Epub Date: 2024-11-20 DOI: 10.3164/jcbn.24-87

Hanna Kavaliova, Shuhei Yamano, Shimon Murahashi, Shuntaro Sato, Yurika Kawazoe, Eri Uemura, Takanobu Otaguro, Hiroo Izumino, Takamitsu Inokuma, Goro Tajima, Kazunori Yamashita, Koichi Hayakawa, Osamu Tasaki

{"title":"Analysis of serum zinc level in patients with severe trauma.","authors":"Hanna Kavaliova, Shuhei Yamano, Shimon Murahashi, Shuntaro Sato, Yurika Kawazoe, Eri Uemura, Takanobu Otaguro, Hiroo Izumino, Takamitsu Inokuma, Goro Tajima, Kazunori Yamashita, Koichi Hayakawa, Osamu Tasaki","doi":"10.3164/jcbn.24-87","DOIUrl":"10.3164/jcbn.24-87","url":null,"abstract":"Zinc plays a role in immune function. This study aimed to investigate the clinical significance of zinc levels in patients with severe trauma by examining the association between serum zinc levels and infectious complications. Inclusion criteria were hospitalization of 2 weeks or more; Abbreviated Injury Scale score of 3 or more; zinc levels measured on admission and at 2 weeks later. Among the 141 included patients on admission, 136 (96.5%) had zinc deficiency, whereas at two weeks later, serum zinc levels increased significantly, with the number of patients with zinc deficiency decreasing to 31 (22.0%). Between the zinc-deficient group and zinc-sufficient group stratified by serum zinc level at two weeks, there were significant differences in lengths of ICU stay and mechanical ventilation, the complication of infection, and positive blood cultures. Logistic regression showed that zinc level at two weeks after admission were significantly associated with the complication of infection (p = 0.03). In addition, age, initial nutrition, Sequential Organ Failure Assessment score, probability of survival, serum phosphate on admission were highly associated with zinc deficiency at two weeks after admission by logistic regression. In the patients with risk factors on admission, zinc supplementation started on the first day of hospitalization might be beneficial.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 3","pages":"296-303"},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0