Journal of Clinical Biochemistry and Nutrition最新文献_第3页

Optimizing vitamin supplementation via reference interval update of vitamins A, E, B1, and B6 using HPLC. 通过高效液相色谱法更新维生素A、E、B1和B6的参考区间，优化维生素补充。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-03-01 Epub Date: 2025-01-22 DOI: 10.3164/jcbn.24-155

Andrea Caballero, Gonzalo Gonzalez-Silva, Pablo Gabriel-Medina, Marc Cuadros, Alfonso Ayora, Albert Blanco-Grau, Víctor Martin-Riera, Laura Conesa, Fernando Moreno, Sarai Garriga-Edo, Lydia Peris-Serra, Clara Sanz-Gea, Yolanda Villena

{"title":"Optimizing vitamin supplementation via reference interval update of vitamins A, E, B1, and B6 using HPLC.","authors":"Andrea Caballero, Gonzalo Gonzalez-Silva, Pablo Gabriel-Medina, Marc Cuadros, Alfonso Ayora, Albert Blanco-Grau, Víctor Martin-Riera, Laura Conesa, Fernando Moreno, Sarai Garriga-Edo, Lydia Peris-Serra, Clara Sanz-Gea, Yolanda Villena","doi":"10.3164/jcbn.24-155","DOIUrl":"10.3164/jcbn.24-155","url":null,"abstract":"Vitamins are essential micronutrients obtained from the diet, required by the body in small amounts daily for proper metabolism. Monitoring their levels is necessary for detecting deficiencies and guiding supplementation in certain clinical conditions. This study aimed to update the reference values for vitamins A, B1, B6, and E, and some related ratios, adjusted to the adult population of our health reference area using liquid chromatography in a direct approach calculation (n = 146, age: 21-64 years, 64% females). No significant differences in vitamin levels or ratios were observed based on age and sex. We obtained reliable and updated reference values: 1.1-2.8 ‍μmol/L and 18.9-42.2 ‍μmol/L for vitamins A and E respectively, 85.9-181.6 nmol/L and 57.0-165.7 nmol/L for vitamins B1 and B6 respectively; and related ratios of 246.2-561.1 ‍ng/g for vitamin B1 corrected by hemoglobin; 5.2-8.9 ‍μmol/mmol and 4.5-7.4 ‍μmol/mmol for vitamin E corrected by cholesterol and total lipids, respectively. These reference values significantly differ from those provided by the reagent manufacturer currently in use. While correcting vitamin E for lipids and vitamin B1 for hemoglobin is not recommended for the general population, these adjustments may be useful in interpreting results in certain pathological conditions.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 2","pages":"148-155"},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The protective role of kakkalide in sepsis-induced intestinal barrier dysfunction via inhibition of NF-κB pathway activation. kakkalide通过抑制NF-κB通路激活在脓毒症诱导的肠屏障功能障碍中的保护作用。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-03-01 Epub Date: 2024-12-05 DOI: 10.3164/jcbn.24-182

Tongrong Xu, Jiahui Han, Nan Wang, Zhirong Huan, Hao Yao, Xin Ge

{"title":"The protective role of kakkalide in sepsis-induced intestinal barrier dysfunction via inhibition of NF-κB pathway activation.","authors":"Tongrong Xu, Jiahui Han, Nan Wang, Zhirong Huan, Hao Yao, Xin Ge","doi":"10.3164/jcbn.24-182","DOIUrl":"10.3164/jcbn.24-182","url":null,"abstract":"Sepsis, a systemic inflammatory response often triggered by infection, can lead to multi-organ failure, with the intestine being one of the most vulnerable organs. The nuclear factor kappa-B (NF-κB) pathway plays a crucial role in immune responses, inflammation, and cell survival, making it central to sepsis-induced intestinal damage. Kakkalide (KA), a bioactive compound known for its anti-inflammatory, cardiovascular, neuroprotective, and anti-diabetic properties, has potential therapeutic effects. However, its impact on sepsis-induced intestinal injury remains unclear. In this study, murine sepsis models were used both in vivo and in vitro to evaluate the protective effects of KA on intestinal histopathology, apoptosis, and inflammation. Results showed that KA significantly reduced intestinal damage and apoptosis, as evidenced by hematoxylin-eosin and TUNEL staining. KA also improved intestinal barrier integrity, as indicated by reduced diamine oxidase activity, d-lactic acid content, and fluorescein isothiocyanate intensity, along with increased expression of zonula occludens-1. Furthermore, KA alleviates inflammation by reducing the levels of tumor necrosis factor-α, interleukin-1β, prostaglandin E2, inducible nitric oxide synthase, and cyclooxygenase-2. Immunofluorescence and Western blot analysis revealed that KA inhibited the sepsis-induced phosphorylation of inhibitor-kappaBα and RelA (P65) and prevented P65's translocation to the nucleus. These findings were confirmed in lipopolysaccharide-induced Caco-2 cells, suggesting that KA protected the intestinal barrier during sepsis by suppressing the NF-κB pathway.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 2","pages":"139-147"},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936737/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dietary coconut oil lowered circulating fetuin-A levels and hepatic expression of fetuin-A in KK/TaJcl mice. 椰子油可降低KK/TaJcl小鼠循环胎儿素a水平和肝脏胎儿素a表达。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-03-01 Epub Date: 2024-10-11 DOI: 10.3164/jcbn.24-160

Yuzuru Iizuka, Chikako Kitagawa, Towa Tamura, Hidehiro Ueshiba, Satoshi Hirako, Toshifumi Osaka, Hyounju Kim, Naoko Yanagisawa

{"title":"Dietary coconut oil lowered circulating fetuin-A levels and hepatic expression of fetuin-A in KK/TaJcl mice.","authors":"Yuzuru Iizuka, Chikako Kitagawa, Towa Tamura, Hidehiro Ueshiba, Satoshi Hirako, Toshifumi Osaka, Hyounju Kim, Naoko Yanagisawa","doi":"10.3164/jcbn.24-160","DOIUrl":"10.3164/jcbn.24-160","url":null,"abstract":"Although coconut oil has attracted great attention as a functional food, enough supportive scientific evidence is lacking. In addition, the beneficial effects of coconut oil consumption on the prevention of metabolic disorders are controversial. Fetuin-A is a plasma glycoprotein secreted by hepatocytes and adipocytes. Circulating fetuin-A levels relate to insulin resistance due to macrophage-mediated adipose tissue inflammation. This study demonstrated that coconut oil feeding significantly downregulated the hepatic expression of fetuin-A and reduced its plasma level in KK mice-an obese diabetic model animal. The expression of monocyte chemoattractant protein-1, a potent inducer for macrophage infiltration, decreased in epididymal white adipose tissue in coconut oil-fed KK mice. The expression of CD68 and CD11c, markers of proinflammatory M1 macrophages, was significantly reduced by coconut oil feeding in epididymal white adipose tissue of KK mice. However, the mice did not exhibit improved insulin resistance. Our results may further support the potential of coconut oil as a dietary trigger that can reduce both circulating fetuin-A levels and infiltration of proinflammatory macrophages in visceral adipose tissue.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 2","pages":"131-138"},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reducing early pregnancy loss with vitamin D₃: an analysis of serum 1,25-(OH)D₃ modulation and miscarriage risk. 减少早期妊娠损失与维生素D3：血清125 -(OH)D3调节和流产风险的分析。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-03-01 Epub Date: 2024-10-29 DOI: 10.3164/jcbn.24-147

Qian Zhao, Liangying Wang, Hongqin Xiang, Luling Qiu

引用次数: 0

Effects of ergothioneine on oxidative DNA damage and immune response induced by circadian rhythm disturbance in mice. 麦角硫因对小鼠昼夜节律紊乱诱导的DNA氧化损伤和免疫应答的影响。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-03-01 Epub Date: 2024-12-26 DOI: 10.3164/jcbn.24-220

Yun-Shan Li, Hiroaki Fujihara, Koichi Fujisawa, Kazuaki Kawai

{"title":"Effects of ergothioneine on oxidative DNA damage and immune response induced by circadian rhythm disturbance in mice.","authors":"Yun-Shan Li, Hiroaki Fujihara, Koichi Fujisawa, Kazuaki Kawai","doi":"10.3164/jcbn.24-220","DOIUrl":"10.3164/jcbn.24-220","url":null,"abstract":"Ergothioneine has antioxidant, anti-inflammatory, and cell-protective properties. Circadian rhythm disruption can lead to health issues, such as insomnia, mental illness, chronic diseases, and cancer. However, the impact of ergothioneine, as an antioxidant, on oxidative DNA damage and immune variations caused by circadian rhythm disruptions remains unclear. To investigate the effect of ergothioneine on oxidative DNA damage and immune responses caused by circadian rhythm disruption, 8-week-old mice were subjected to night time feeding and exercise restrictions for 14 days. Body weight, daytime running wheel activity, and anxiety-like behavior showed no significant differences between the night-restricted groups, regardless of ergothioneine administration. Serum interleukin-6 levels, 8-hydroxy-2'-deoxyguanosine levels in urine and nuclear DNA of the liver, testes, lungs, and pancreas were significantly reduced in the night-restricted group receiving ergothioneine compared with that of the group without ergothioneine, with no significant differences observed when compared to the control group. Ergothioneine can mitigate immune function changes and oxidative DNA damage induced by circadian rhythm disruption caused by abnormal dietary timing in mice. However, it did not alleviate obesity or mental state dysregulation. These findings have important implications for improving night-shift workers health and developing therapies for diseases associated with circadian rhythm disturbances.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 2","pages":"117-124"},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936743/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Short-term intake of delta-tocotrienol on lipid profiles in healthy subjects. 短期摄入-生育三烯醇对健康受试者血脂的影响。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-01-01 Epub Date: 2024-10-11 DOI: 10.3164/jcbn.24-124

Naho Serizawa, Yusuke Ninomiya, Akiho Shinagawa, Ayako Minematsu, Yuichi Miyakoshi, Tomohiro Yano, Masako Ota

{"title":"Short-term intake of delta-tocotrienol on lipid profiles in healthy subjects.","authors":"Naho Serizawa, Yusuke Ninomiya, Akiho Shinagawa, Ayako Minematsu, Yuichi Miyakoshi, Tomohiro Yano, Masako Ota","doi":"10.3164/jcbn.24-124","DOIUrl":"10.3164/jcbn.24-124","url":null,"abstract":"Delta-tocotrienol (δ-T3) are beneficial for lipid metabolism. However, there are almost no reports on the specific numerical values of changes in blood tocotrienol levels when Japanese people consume tocotrienol. To observe the effect on blood δ-T3 and lipid concentrations after taking δ-T3, a crossover test was conducted with a control group, alpha-tocopherol (α-TP) group (264 ‍mg/day), and δ-T3 group (250 ‍mg/day). Participants took the target supplements for 14 days. This study revealed that plasma δ-T3 concentrations in healthy men and women increased significantly even with short-term intake (pre; 0.11 ± 0.03 to post; 0.40 ± 0.19 ‍μmol/L). The results of this study showed that α-TP and δ-T3 intake did not significantly affect any of the lipid profiles. However, the intake of δ-T3 tended to increase Low-density lipoprotein cholesterol (HDL-C) levels (pre: 59 ± 17 to post: 64 ± 20 ‍mg/dl). These results provide new evidence on changes in blood δ-T3 concentrations with δ-T3 intake in young people and suggest the potential of δ-T3 for the prevention of atherosclerosis.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 1","pages":"3-7"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11782774/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143079195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell analysis reveals islet autoantigen's immune activation in type 1 diabetes patients. 单细胞分析揭示1型糖尿病患者胰岛自身抗原的免疫激活。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-01-01 Epub Date: 2024-08-29 DOI: 10.3164/jcbn.24-86

Takuro Okamura, Noriyuki Kitagawa, Nobuko Kitagawa, Kimiko Sakai, Madoka Sumi, Genki Kobayashi, Dan Imai, Takaaki Matsui, Masahide Hamaguchi, Michiaki Fukui

{"title":"Single-cell analysis reveals islet autoantigen's immune activation in type 1 diabetes patients.","authors":"Takuro Okamura, Noriyuki Kitagawa, Nobuko Kitagawa, Kimiko Sakai, Madoka Sumi, Genki Kobayashi, Dan Imai, Takaaki Matsui, Masahide Hamaguchi, Michiaki Fukui","doi":"10.3164/jcbn.24-86","DOIUrl":"10.3164/jcbn.24-86","url":null,"abstract":"In this study, we used single-cell sequencing, which can comprehensively detect the type and number of transcripts per cell, to efficiently stimulate peripheral blood mononuclear cells of type 1 diabetic patients with overlapping peptides of GAD, IA-2, and insulin antigens, and performed gene expression analysis by single-cell variable-diversity-joining sequencing and T-cell receptor repertoire analysis. Twenty male patients with type 1 diabetes mellitus participating in the KAMOGAWA-DM cohort were included. Four of them were randomly selected for BD Rhapsody system after reacting peripheral blood mononuclear cells with overlapping peptides of GAD, IA-2, and insulin antigen. Peripheral blood mononuclear cells were clustered into CD8+ T cells, CD4+ T cells, granulocytes, natural killer cells, dendritic cells, monocytes, and B cells based on Seurat analysis. In the insulin group, gene expression of inflammatory cytokines was elevated in cytotoxic CD8+ T cells and Th1 and Th17 cells, and gene expression related to exhaustion was elevated in regulatory T cells. In T cell receptors of various T cells, the T cell receptor β chain was monoclonally increased in the TRBV28/TRBJ2-7 pairs. This study provides insights into the pathogenesis of type 1 diabetes and provides potential targets for the treatment of type 1 diabetes.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 1","pages":"64-84"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11782777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143079367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fat-free oral nutritional supplements for patients after acute illness: a prospective observational study. 急性疾病后患者的无脂口服营养补充剂：一项前瞻性观察研究。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-01-01 Epub Date: 2024-10-11 DOI: 10.3164/jcbn.24-162

Kensuke Nakamura, Yuji Takahashi, Yusuke Yanai, Tomoka Miyagi, Hidehiko Nakano, Yasuaki Koyama, Hideki Hashimoto

{"title":"Fat-free oral nutritional supplements for patients after acute illness: a prospective observational study.","authors":"Kensuke Nakamura, Yuji Takahashi, Yusuke Yanai, Tomoka Miyagi, Hidehiko Nakano, Yasuaki Koyama, Hideki Hashimoto","doi":"10.3164/jcbn.24-162","DOIUrl":"10.3164/jcbn.24-162","url":null,"abstract":"There is a lack of evidence for compliance with and the acceptability of oral nutritional supplements (ONS) by post-acute care patients. Therefore, the present study examined compliance with fat-free ONS, which are easy to drink. Patients who started oral intake in the general ward after being transferred from the Emergency Department were offered three ONS including fat-free ONS: Isocal Clear, Maybalance Mini, and Medimil, three times a day for three days. On days 1 and 3, patients evaluated each ONS using a questionnaire. Thirty-five eligible patients participated in the present study, which began a median of 10 days after their admission. Median taste ratings for Isocal Clear, Maybalance, and Medimil on day 1 were 8, 7, and 3, respectively, while median ease-to-drink ratings were 8, 7, and 5, respectively. In contrast, median taste ratings on day 3 were 5, 0, and 0, respectively, while median ease-to-drink ratings were 7, 1, and 0, respectively. Intakes of the prescribed diet during the three days had a median value as low as 30-50%. In conclusion, good compliance with fat-free ONS by post-acute care patients may be achieved.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 1","pages":"85-89"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11782773/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LC/MS/MS detection of uric acid oxidation metabolites to evaluate reactive oxygen and nitrogen species in vivo with application to pseudo-inflammation. LC/MS/MS检测尿酸氧化代谢产物评价体内活性氧和活性氮种类及其在假性炎症中的应用。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-01-01 Epub Date: 2024-11-09 DOI: 10.3164/jcbn.24-152

Yuka Yokozawa, Haruki Watanabe, Aya Matsubara, Rina Horinouchi, Sayaka Iida, Yorihiro Yamamoto, Akio Fujisawa

{"title":"LC/MS/MS detection of uric acid oxidation metabolites to evaluate reactive oxygen and nitrogen species in vivo with application to pseudo-inflammation.","authors":"Yuka Yokozawa, Haruki Watanabe, Aya Matsubara, Rina Horinouchi, Sayaka Iida, Yorihiro Yamamoto, Akio Fujisawa","doi":"10.3164/jcbn.24-152","DOIUrl":"10.3164/jcbn.24-152","url":null,"abstract":"Uric acid, a water-soluble antioxidant ubiquitous in human bodily fluids at relatively high concentrations, reacts with a variety of reactive oxygen and nitrogen species. Ours and other previous studies identified allantoin, oxaluric acid, triuret, and 5-N-carboxyimino-6-aminopyrimidine-2,4(3H)-dione as specific metabolites reactive against free radicals, singlet oxygen, peroxynitrite, and hypochlorous anion, respectively. We analyzed human plasma spiked with these products using high-performance liquid chromatography-tandem mass spectrometry. We observed recoveries of 40-110% and coefficients of variance within 7%. Samples remained stable at -80°C for at least 4 weeks, indicating the analytical method is sound. Detection of these metabolites in biological samples enables the identification of each species generated in vivo. We observed changes in the products in human blood during pseudo-inflammation induced by treatment with lipopolysaccharide. Levels of allantoin, oxaluric acid, triuret, and 5-N-carboxyimino-6-aminopyrimidine-2,4(3H)-dione increased after addition of lipopolysaccharide. The formation of singlet oxygen was confirmed by increased formation of Trp-derived cis-hydroxide ([2S,3aR,8aR]-3a-hydroxy-1,2,3,3a,8,8a-hexahydropyrrolo[2,3-b]indole-2-carboxylic acid) (cis-WOH). We believe our method will aid development of strategies to treat oxidative stress-associated diseases.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 1","pages":"8-15"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11782772/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MiR-301b-3p targets and regulates EBF3 to impact the stem-like phenotype of breast cancer cells through glycolysis. MiR-301b-3p通过糖酵解作用靶向并调控EBF3，影响乳腺癌细胞的干样表型。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-01-01 Epub Date: 2024-10-29 DOI: 10.3164/jcbn.23-131

Jiankang Huang, Weidong Zhen, Xiaokai Ma, Suxia Ge, Ling Ma

{"title":"MiR-301b-3p targets and regulates EBF3 to impact the stem-like phenotype of breast cancer cells through glycolysis.","authors":"Jiankang Huang, Weidong Zhen, Xiaokai Ma, Suxia Ge, Ling Ma","doi":"10.3164/jcbn.23-131","DOIUrl":"10.3164/jcbn.23-131","url":null,"abstract":"Background: Cancer stem cells are essential for the development of tumors, their recurrence, metastasis, and resistance to treatment. Previous studies have shown that the silencing of EBF3 promotes the progression of malignant tumors, but its impact on the stem-like phenotype of tumor cells remains unexplored. Therefore, this work aims to investigate the influence of EBF3 on the stem-like phenotype of breast cancer (BC) cells and its underlying molecular mechanisms.Methods: Bioinformatics analysis was utilized to predict EBF3 and miR-301b-3p expression and their binding sites in BC tissues. qRT-PCR was conducted to assess EBF3 and miR-301b-3p expression in BC cells. Cell viability was assessed using CCK-8 assay, while sphere-forming ability was assayed by sphere formation experiments. Western blot analysis was employed to assess the expression of stem cell-related markers and proteins associated with the glycolysis metabolic pathway. ECAR experiments and analysis of glycolysis metabolite production were performed to evaluate cellular glycolysis capacity. Dual-luciferase reporter assays and RIP were utilized to validate the binding relationship between EBF3 and miR-301b-3p.Results: EBF3 was downregulated in BC tissues and cells, and overexpression of EBF3 repressed the glycolysis capacity of BC cells, thereby suppressing stem-like phenotype. Furthermore, miR-301b-3p was identified as a direct target of EBF3, and its expression was increased in BC. Cell experiments revealed that miR-301b-3p suppressed EBF3 expression, thereby promoting the glycolysis capacity and stem-like phenotype of BC cells.Conclusion: miR-301b-3p enhanced glycolysis and promoted the stem-like phenotype of BC cells by targeting EBF3. These findings can offer new therapeutic approaches for BC.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 1","pages":"25-34"},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11782780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0