Journal of Clinical Biochemistry and Nutrition最新文献_第10页

The nitrogen load is affected by high protein provision according to kidney function in critically ill patients. 危重病人根据肾功能不同，高蛋白供给对氮负荷有影响。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-05-01 DOI: 10.3164/jcbn.22-87

Masaki Mochizuki, Hidehiko Nakano, Daisuke Ikechi, Yuji Takahashi, Hideki Hashimoto, Kensuke Nakamura

{"title":"The nitrogen load is affected by high protein provision according to kidney function in critically ill patients.","authors":"Masaki Mochizuki, Hidehiko Nakano, Daisuke Ikechi, Yuji Takahashi, Hideki Hashimoto, Kensuke Nakamura","doi":"10.3164/jcbn.22-87","DOIUrl":"https://doi.org/10.3164/jcbn.22-87","url":null,"abstract":"Adequate protein delivery is recommended in the acute phase of critical illness with kidney dysfunction. However, the influence of the protein and nitrogen loads has not yet been clarified. Patients admitted to the intensive care unit were included. In the former period, patients received standard care (0.9 g/kg/day protein). In the latter, patients received the intervention of active nutrition therapy with high protein delivery (1.8 g/kg/day protein). Fifty patients in the standard care group and 61 in the intervention group were examined. Maximum blood urea nitrogen (BUN) on days 7-10 were 27.9 (17.3, 38.6) vs 33 (26.3, 51.8) (mg/dl) (p = 0.031). The maximum difference in BUN increased [31.3 (22.8, 55) vs 50 (37.3, 75.9) mg/dl (p = 0.047)] when patients were limited to an estimated glomerular filtration rate (eGFR) <50 ml/min/1.73 m2. This difference increased further when patients were limited to eGFR <30 ml/min/1.73 m2. No significant differences were observed in maximum Cre or in the use of RRT. In conclusion, the provision of 1.8 g/kg/day protein was associated with an increase in BUN in critically ill patients with kidney dysfunction; however, it was tolerated without the need for RRT.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"72 3","pages":"289-294"},"PeriodicalIF":2.4,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/11/3c/jcbn22-87.PMC10209593.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9539751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Method for detecting CoQ10 incorporation in the mitochondrial respiratory chain supercomplex. 线粒体呼吸链超复合体中辅酶q10掺入的检测方法。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-05-01 DOI: 10.3164/jcbn.22-137

Kyousuke Sugawara, Seiji Sato, Yuto Tanaka, Akari Nakamura, Akio Fujisawa, Yorihiro Yamamoto, Misato Kashiba

{"title":"Method for detecting CoQ10 incorporation in the mitochondrial respiratory chain supercomplex.","authors":"Kyousuke Sugawara, Seiji Sato, Yuto Tanaka, Akari Nakamura, Akio Fujisawa, Yorihiro Yamamoto, Misato Kashiba","doi":"10.3164/jcbn.22-137","DOIUrl":"https://doi.org/10.3164/jcbn.22-137","url":null,"abstract":"Coenzyme Q10 is an important component of the mitochondrial electron transfer chain. A supercomplex of mitochondrial electron transfer system proteins exists. This complex also contains coenzyme Q10. The concentrations of coenzyme Q10 in tissues decrease with age and pathology. Coenzyme Q10 is given as a supplement. It is unknown whether coenzyme Q10 is transported to the supercomplex. We develop a method for measuring coenzyme Q10 in the mitochondrial respiratory chain supercomplex in this study. Blue native electrophoresis was used to separate mitochondrial membranes. Electrophoresis gels were cut into 3 mm slices. Hexane was used to extract coenzyme Q10 from this slice, and HPLC-ECD was used to analyze coenzyme Q10. Coenzyme Q10 was found in the gel at the same site as the supercomplex. Coenzyme Q10 at this location was thought to be coenzyme Q10 in the supercomplex. We discovered that 4-nitrobenzoate, a coenzyme Q10 biosynthesis inhibitor, reduced the amount of coenzyme Q10 both within and outside the supercomplex. We also observed that the addition of coenzyme Q10 to cells increased the amount of coenzyme Q10 in the supercomplex. It is expected to analysis coenzyme Q10 level in supercomplex in various samples by using this novel method.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"72 3","pages":"207-214"},"PeriodicalIF":2.4,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b6/e1/jcbn22-137.PMC10209600.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9547251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of the oxidative profiles before and after revascularization in peripheral arterial disease: a pilot study. 外周动脉疾病血运重建前后氧化谱的比较:一项初步研究

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-05-01 DOI: 10.3164/jcbn.22-109

Kozo Takeuchi, Kimiko Kazumura, Akihiro Yoshida, Tappei Furuta, Kazunori Hayashi, Masashi Nagai, Yukiko Hatano, Michitaka Naito, Etsushi Matsushita

{"title":"Comparison of the oxidative profiles before and after revascularization in peripheral arterial disease: a pilot study.","authors":"Kozo Takeuchi, Kimiko Kazumura, Akihiro Yoshida, Tappei Furuta, Kazunori Hayashi, Masashi Nagai, Yukiko Hatano, Michitaka Naito, Etsushi Matsushita","doi":"10.3164/jcbn.22-109","DOIUrl":"https://doi.org/10.3164/jcbn.22-109","url":null,"abstract":"Reactive and highly reactive oxygen species (ROS and hROS) produced by white blood cells are essential for innate immunity; however, they may cause oxidative stress in the host. We developed systems for simultaneously monitoring ROS and hROS, i.e., superoxide radicals (O2•-) and hypochlorite ions (OCl-) secreted from stimulated white blood cells in a few microliters of whole blood. We previously reported on the evaluation of healthy volunteers' blood using the developed system; however, whether patients' blood can be assessed remains unclear. Here, we report a pilot study of 30 cases (28 patients) with peripheral arterial disease, in whom we measured the ROS and hROS levels before and approximately one month after endovascular treatment (EVT) using the system (CFL-H2200) that we developed. At approximately the same time points, physiological indices of blood vessels, oxidative stress markers, and standard clinical parameters in the blood were also monitored. The ankle-brachial index, a diagnostic tool for peripheral arterial disease, was significantly improved after EVT (p<0.001). The ROS-hROS ratio, low-density lipoprotein cholesterol, and hematocrit levels were decreased after EVT (p<0.05), while triglyceride and lymphocyte levels were increased after EVT (p<0.05). The correlations between the study parameters were also analyzed.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"72 3","pages":"278-288"},"PeriodicalIF":2.4,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9b/fe/jcbn22-109.PMC10209599.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9545313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased expression of ELOVL7 contributes to production of inflammatory cytokines in THP-1 cell-derived M1-like macrophages. ELOVL7的表达增加有助于THP-1细胞来源的m1样巨噬细胞产生炎症细胞因子。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-05-01 DOI: 10.3164/jcbn.22-69

Yuki Inoue, Tetsuro Kamiya, Hirokazu Hara

{"title":"Increased expression of ELOVL7 contributes to production of inflammatory cytokines in THP-1 cell-derived M1-like macrophages.","authors":"Yuki Inoue, Tetsuro Kamiya, Hirokazu Hara","doi":"10.3164/jcbn.22-69","DOIUrl":"https://doi.org/10.3164/jcbn.22-69","url":null,"abstract":"The elevation of intracellular very long-chain fatty acids (VLCFAs) augments pro-inflammatory activity of macrophages. VLCFAs are considered to function as regulators in macrophage inflammatory responses; however, the precise mechanism of regulating the production of VLCFAs is unclear. In this study, we focused on elongation of the very‑long‑chain fatty acid protein (ELOVL) family, rate-determining enzymes for VLCFA synthesis, in macrophages. ELOVL7 mRNA was upregulated in human monocytic THP-1 cell-derived M1-like macrophages. Metascape analysis using the RNA-seq data set showed the involvement of NF-κB and STAT1 in transcriptional regulation of ELOVL7 highly correlated genes. Gene ontology (GO) enrichment analysis suggested that ELOVL7 highly correlated genes were closely associated with multiple pro-inflammatory responses, including response to virus and positive regulation of NF-κB signaling. Consistent with RNA-seq analysis, the NF-κB inhibitor BAY11-7082, but not the STAT1 inhibitor fludarabine, canceled ELOVL7 upregulation in M1-like macrophages. ELOVL7 knockdown decreased interleukin (IL)-6 and IL-12/IL-23 p40 production. Moreover, RNA-seq analysis of plasmacytoid dendritic cells (pDCs) revealed that ELOVL7 was upregulated in pDCs treated with TLR7 and TLR9 agonists. In conclusion, we propose that ELOVL7 is a novel pro-inflammatory gene that is upregulated by inflammatory stimuli, and regulates M1-like macrophage and pDC functions.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"72 3","pages":"215-224"},"PeriodicalIF":2.4,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a2/f0/jcbn22-69.PMC10209594.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9539749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Pharmacokinetics and effect of maslinic acid with physical exercise on grip strength and trunk muscle mass in healthy Japanese individuals. 运动对日本健康人握力和躯干肌肉质量的药代动力学及山楂酸的影响

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-05-01 DOI: 10.3164/jcbn.23-5

Yuki Yamauchi, Tetsu Kinoshita, Satoshi Fukumitsu, Kazuhiko Aida, Koutatsu Maruyama, Isao Saito, Naofumi Yamamoto

{"title":"Pharmacokinetics and effect of maslinic acid with physical exercise on grip strength and trunk muscle mass in healthy Japanese individuals.","authors":"Yuki Yamauchi, Tetsu Kinoshita, Satoshi Fukumitsu, Kazuhiko Aida, Koutatsu Maruyama, Isao Saito, Naofumi Yamamoto","doi":"10.3164/jcbn.23-5","DOIUrl":"https://doi.org/10.3164/jcbn.23-5","url":null,"abstract":"Age-related changes in physical function are closely associated with daily activity impairment among the elderly. Continuous maslinic acid intake may improve skeletal muscle mass; however, the concentration-dependent benefits of maslinic acid for physical functionality remain unclear. Therefore, we evaluated the bioavailability of maslinic acid and examined the effect of maslinic acid intake on skeletal muscle and quality of life in the healthy Japanese elderly. Five healthy adult men were administered test diets containing 30, 60, or 120 mg of maslinic acid. Analysis of plasma maslinic acid revealed concentration-dependent elevations in blood maslinic acid levels (p<0.01). Next, 69 healthy Japanese adult men and women were administered a placebo or 30 or 60 mg of maslinic acid continuously for 12 weeks with physical exercise in a randomized, double-blind, placebo-controlled trial. The trunk muscle mass (p<0.05) and vitality score according to the Short-Form-8 (p<0.05) were significantly higher in the 60 mg maslinic acid group than in the placebo group. Additionally, grip strength was significantly higher in the 30 (p<0.05) and 60 mg (p<0.05) groups than in the placebo group. Overall, maslinic acid intake with physical exercise improved muscle strength, muscle mass, and quality of life in a maslinic acid-intake-dependent manner.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"72 3","pages":"270-277"},"PeriodicalIF":2.4,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/63/9d/jcbn23-5.PMC10209603.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9547250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of exposure and DNA damage from second-hand smoke using potential biomarker in urine: cigarettes and heated tobacco products. 利用尿液中潜在的生物标志物评估二手烟暴露和DNA损伤:香烟和加热烟草制品。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-05-01 DOI: 10.3164/jcbn.22-144

Yuya Kawasaki, Yun-Shan Li, Yuko Ootsuyama, Koichi Fujisawa, Hisamitsu Omori, Ayumi Onoue, Kenichi Kubota, Toshimi Yoshino, Yoshio Nonami, Minoru Yoshida, Hiroshi Yamato, Kazuaki Kawai

{"title":"Assessment of exposure and DNA damage from second-hand smoke using potential biomarker in urine: cigarettes and heated tobacco products.","authors":"Yuya Kawasaki, Yun-Shan Li, Yuko Ootsuyama, Koichi Fujisawa, Hisamitsu Omori, Ayumi Onoue, Kenichi Kubota, Toshimi Yoshino, Yoshio Nonami, Minoru Yoshida, Hiroshi Yamato, Kazuaki Kawai","doi":"10.3164/jcbn.22-144","DOIUrl":"https://doi.org/10.3164/jcbn.22-144","url":null,"abstract":"Second-hand smoke exposure is an established cause of several adverse health effects. Tobacco smoke exposure in the environment has been improved by the WHO Framework Convention on Tobacco Control. However, concerns have been raised regarding the health effects of heated tobacco products. Analysis of tobacco smoke biomarkers is critical for assessing the health effects of second-hand tobacco smoke exposure. In this study, nicotine metabolites (nicotine, cotinine, trans-3'-hydroxycotinine) and carcinogenic 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol were analysed in the urine of non-smokers with or without passive exposure to cigarettes and heated tobacco products. In addition, 7-methylguanine and 8-hydroxy-2'-deoxyguanosine were simultaneously measured as DNA damage markers. The results revealed higher levels of urinary nicotine metabolites and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol in participants exposed to second-hand tobacco smoke (both cigarettes and heated tobacco products) at home. In addition, the urinary levels of 7-methylguanine and 8-hydroxy-2'-deoxyguanosine tended to be higher in the second-hand tobacco smoke-exposed group. The urinary levels of nicotine metabolites and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol were high in workplaces with no protection against passive smoking. These biomarkers will be useful for evaluating passive exposure to tobacco products.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"72 3","pages":"242-247"},"PeriodicalIF":2.4,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/43/38/jcbn22-144.PMC10209597.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9539752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A newly developed solution for the preservation of short-chain fatty acids, bile acids, and microbiota in fecal specimens. 一种用于保存粪便标本中的短链脂肪酸、胆汁酸和微生物群的新开发的溶液。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-05-01 DOI: 10.3164/jcbn.22-107

Tomohisa Takagi, Tadao Kunihiro, Shunsuke Takahashi, Takayoshi Hisada, Koji Nagashima, Jun Mochizuki, Katsura Mizushima, Yuji Naito

{"title":"A newly developed solution for the preservation of short-chain fatty acids, bile acids, and microbiota in fecal specimens.","authors":"Tomohisa Takagi, Tadao Kunihiro, Shunsuke Takahashi, Takayoshi Hisada, Koji Nagashima, Jun Mochizuki, Katsura Mizushima, Yuji Naito","doi":"10.3164/jcbn.22-107","DOIUrl":"https://doi.org/10.3164/jcbn.22-107","url":null,"abstract":"Recent studies have revealed that the gut microbiome affects various health conditions via its metabolites, including short-chain fatty acids (SCFAs) and bile acids (BAs). In the analysis of these, appropriate collection, handling, and storage of fecal specimens are required, and convenient specimen handling processes will facilitate their investigation. Here, we developed a novel preservation solution, \"Metabolokeeper®\", to stabilize fecal microbiota, organic acids including SCFAs, and BAs at room temperature. In the present study, we collected fecal samples from 20 healthy adult volunteers and stored them at room temperature with Metabolokeeper® and at -80°C without preservatives for up to four weeks to evaluate the usefulness of the novel preservative solution. We found that microbiome profiles and short chain fatty acid contents were stably maintained at room temperature with Metabolokeeper® for 28 days, while the bile acids were stably maintained for 7 days under the same conditions. We conclude that this convenient procedure to obtain a fecal sample for collecting the gut microbiome and gut metabolites can contribute to a better understanding of the health effects of fecal metabolites produced by the gut microbiome.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"72 3","pages":"263-269"},"PeriodicalIF":2.4,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/30/50/jcbn22-107.PMC10209596.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9539753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A change-point regression approach for estimating no observed adverse effect level from systematic review. 用于估计系统评价中未观察到的不良反应水平的变点回归方法。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-05-01 DOI: 10.3164/jcbn.22-128

Yui Kuramochi, Kohsuke Hayamizu

{"title":"A change-point regression approach for estimating no observed adverse effect level from systematic review.","authors":"Yui Kuramochi, Kohsuke Hayamizu","doi":"10.3164/jcbn.22-128","DOIUrl":"https://doi.org/10.3164/jcbn.22-128","url":null,"abstract":"Systematic reviews can be used not only to evaluate the efficacy and usefulness of a drug or food ingredient, but also as a safety assessment method. One of the aims of safety assessment is to estimate the no observed adverse effect level and the lowest observed adverse effect level. However, no methodology to statistically estimate the no observed adverse effect level from systematic review results has yet been reported. Estimation of the no observed adverse effect level involves a search for the dose above which adverse events occur is even exploration of the thresholds in dose response. To search for the dose above which adverse events occur, we examined an estimation method using the weighted change-point regression model, which includes the weights of each study used for systematic reviews in the model. This model could be applied to safety data of an omega-3 study in the form of a systematic review. We demonstrated that the dose response to omega-3 intake regarding adverse events had a threshold value and that the no observed adverse effect level could be estimated using the developed model.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"72 3","pages":"256-262"},"PeriodicalIF":2.4,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/36/54/jcbn22-128.PMC10209598.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9547249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Transferrin, insulin, and progesterone modulate intracellular concentrations of coenzyme Q and cholesterol, products of the mevalonate pathway, in undifferentiated PC12 cells. 在未分化的PC12细胞中，转铁蛋白、胰岛素和黄体酮调节辅酶Q和胆固醇的细胞内浓度，它们是甲羟戊酸途径的产物。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-05-01 DOI: 10.3164/jcbn.22-115

Akari Nakamura, Yukina Aida, Mizuho Okamoto, Ayaka Maeda, Ayaka Nagao, Kanae Kitatani, Susumu Takekoshi, Akio Fujisawa, Yorihiro Yamamoto, Misato Kashiba

{"title":"Transferrin, insulin, and progesterone modulate intracellular concentrations of coenzyme Q and cholesterol, products of the mevalonate pathway, in undifferentiated PC12 cells.","authors":"Akari Nakamura, Yukina Aida, Mizuho Okamoto, Ayaka Maeda, Ayaka Nagao, Kanae Kitatani, Susumu Takekoshi, Akio Fujisawa, Yorihiro Yamamoto, Misato Kashiba","doi":"10.3164/jcbn.22-115","DOIUrl":"https://doi.org/10.3164/jcbn.22-115","url":null,"abstract":"Coenzyme Q (CoQ) is important not only as an essential lipid for the mitochondrial electron transport system, but also as an antioxidant. CoQ levels decrease during aging and in various diseases. Orally administered CoQ is not readily taken up in the brain, so it is necessary to develop a method to increase the amount of CoQ in neurons. CoQ is synthesized via mevalonate pathway, like cholesterol. Transferrin, insulin, and progesterone are factors used in the culture of neurons. In this study, we determined the effect of these reagents on cellular CoQ and cholesterol levels. The administration of transferrin, insulin, and progesterone increased cellular CoQ levels in undifferentiated PC12 cells. When serum was removed and only insulin was administered, intracellular CoQ levels increased. This increase was even more pronounced with concurrent administration of transferrin, insulin, and progesterone. Cholesterol level decreased by the administration of transferrin, insulin, and progesterone. Progesterone treatment lowered intracellular cholesterol levels in a concentration-dependent manner. Our findings suggest that transferrin, insulin, and progesterone may be useful in regulating CoQ levels and cholesterol levels, which are products of the mevalonate pathway.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"72 3","pages":"199-206"},"PeriodicalIF":2.4,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/99/36/jcbn22-115.PMC10209592.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9545310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HIF1α/CCL7/KIAA1199 axis mediates hypoxia-induced gastric cancer aggravation and glycolysis alteration. HIF1α/CCL7/KIAA1199轴介导缺氧诱导的胃癌加重和糖酵解改变。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-05-01 DOI: 10.3164/jcbn.22-48

Chen Mi, Yan Zhao, Li Ren, Dan Zhang

{"title":"HIF1α/CCL7/KIAA1199 axis mediates hypoxia-induced gastric cancer aggravation and glycolysis alteration.","authors":"Chen Mi, Yan Zhao, Li Ren, Dan Zhang","doi":"10.3164/jcbn.22-48","DOIUrl":"https://doi.org/10.3164/jcbn.22-48","url":null,"abstract":"Gastric cancer is a common digestion tumor with high malignant severity and prevalence. Emerging studies reported C-C motif chemokine ligand 7 (CCL7) as a regulator of various tumor diseases. Our research explored the function and underlying mechanism of CCL7 during gastric cancer development. RT-qPCR, Western blot and other datasets were employed to evaluate CCL7 expression in tissues and cells. Kaplan-Meier and Cox regression analyses were recruited to evaluate the correlations between CCL7 expression and patients' survival or clinical features. A loss-of-function assay was performed to evaluate the function of CCL7 in gastric cancer. 1% O2 was utilized to mimic hypoxic condition. KIAA1199 and HIF1α were included in the regulatory mechanism. The results showed that CCL7 was up-regulated and its high expression was correlated with poor survival of gastric cancer patients. Depressing CCL7 attenuated proliferation, migration, invasion, and induced apoptosis of gastric cancer cells. Meanwhile, CCL7 inhibition weakened hypoxia-induced gastric cancer aggravation. Besides, KIAA1199 and HIF1α were involved in the mechanism of CCL7-mediated gastric cancer aggravation under hypoxia. Our research identified CCL7 as a novel tumor-activator in gastric cancer pathogenesis and hypoxia-induced tumor aggravation was regulated by HIF1α/CCL7/KIAA1199 axis. The evidence may provide a novel target for gastric cancer treatment.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"72 3","pages":"225-233"},"PeriodicalIF":2.4,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/50/e9/jcbn22-48.PMC10209595.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9539746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0