Journal of Clinical Biochemistry and Nutrition最新文献_第10页

Nutritional status and body composition of patients with head and neck cancer treated with perioperative evidence-based nutritional management. 接受围手术期循证营养管理治疗的头颈部癌症患者的营养状况和身体组成。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2024-07-01 Epub Date: 2024-05-21 DOI: 10.3164/jcbn.24-35

Zhen Ding, Lingmei Zhou, Yan Zhou, Kemei Jin, Runjinxing Wu, Yihua Gui

{"title":"Nutritional status and body composition of patients with head and neck cancer treated with perioperative evidence-based nutritional management.","authors":"Zhen Ding, Lingmei Zhou, Yan Zhou, Kemei Jin, Runjinxing Wu, Yihua Gui","doi":"10.3164/jcbn.24-35","DOIUrl":"10.3164/jcbn.24-35","url":null,"abstract":"We aimed to describe nutritional status and body composition profiles perioperative head and neck cancer (HNC) patients managed with whole-course nutritional support. Scored Nutritional Risk Screening (NRS 2002), Patient-Generated Subjective Global Assessment (PG-SGA), and body composition were conducted. The factors related to weight loss and skeletal muscle mass (SMM) were identified. Lower weight and body composition levels in low skeletal muscle index (SMI≤9.90 kg/m2) group were observed. Levels of albumin, prealbumin, prognostic nutritional index (PNI), and lymphocyte-to-monocyte ratio (LMR) were lower than pre-operative, but the values after 2 weeks were higher than 1 week post-operatively (all p<0.01). The neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) were increased at 1 and 2 weeks post-operative compared to pre-operative (both p<0.01). Post-operatively, NLR at 2 weeks was lowed than 1 week (p = 0.02). A negative correlation was observed between SMM loss and serum prealbumin (r = -0.255, p = 0.029). Pre-operative BMI (p<0.01), tumor differentiation (p = 0.003), and nutritional risk (p = 0.049) were risk factors for weight loss. In conclusions, for perioperative HNC patients, loss of adipose tissue occurred earlier than muscle. Prealbumin should be considered as an indicator for monitoring of recovery in clinical practice.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"75 1","pages":"71-77"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11273269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reciprocal regulation of protein arginine deiminase 2 and 4 expression in the colonic mucosa of ulcerative colitis. 溃疡性结肠炎结肠粘膜中精氨酸脱氨酶蛋白 2 和 4 表达的相互调控。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2024-07-01 Epub Date: 2023-12-19 DOI: 10.3164/jcbn.23-77

Yasuo Otsuka, Yasuhiro Masuta, Kosuke Minaga, Natsuki Okai, Akane Hara, Ryutaro Takada, Sho Masaki, Ken Kamata, Hajime Honjo, Kouhei Yamashita, Masatoshi Kudo, Tomohiro Watanabe

{"title":"Reciprocal regulation of protein arginine deiminase 2 and 4 expression in the colonic mucosa of ulcerative colitis.","authors":"Yasuo Otsuka, Yasuhiro Masuta, Kosuke Minaga, Natsuki Okai, Akane Hara, Ryutaro Takada, Sho Masaki, Ken Kamata, Hajime Honjo, Kouhei Yamashita, Masatoshi Kudo, Tomohiro Watanabe","doi":"10.3164/jcbn.23-77","DOIUrl":"10.3164/jcbn.23-77","url":null,"abstract":"Neutrophils express protein arginine deiminase 2 and PAD4, both of which mediate the citrullination of target proteins to induce production of neutrophil extracellular traps. Although PAD-dependent NETs trigger inflammatory bowel disease, the mechanisms governing the expression of PAD2 and PAD4 are poorly understood. In this study, we tried to clarify expression mechanisms of PAD2 and PAD4 in the colonic mucosa of patients with ulcerative colitis and Crohn's disease. Administration of Cl-amidine, a pan PAD-inhibitor, attenuated the development of dextran sodium sulfate-induced colitis, the effects of which were accompanied by reduced IL-6 and TNF-α production by colonic lamina propria mononuclear cells upon exposure to Toll-like receptor ligands. The mRNA expression of colonic PAD2 and PAD4 was negatively and positively correlated with disease activity and pro-inflammatory cytokine responses in patients with UC, respectively. Reciprocal regulation of PAD2 and PAD4 mRNA expression was observed in the colonic mucosa of UC patients, but not in those of CD patients. PAD4 mRNA expression was correlated with disease activity and pro-inflammatory cytokine responses in patients with CD. Collectively, these data suggest that reciprocal regulation of PAD2 and PAD4 expression is associated with disease activity in UC patients.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"75 1","pages":"46-53"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11273265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

National trends in hospitalizations for gastrointestinal bleeding in Japan. 日本全国因消化道出血住院的趋势。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2024-07-01 Epub Date: 2024-02-28 DOI: 10.3164/jcbn.23-111

Chikamasa Ichita, Tadahiro Goto, Akiko Sasaki, Sayuri Shimizu

{"title":"National trends in hospitalizations for gastrointestinal bleeding in Japan.","authors":"Chikamasa Ichita, Tadahiro Goto, Akiko Sasaki, Sayuri Shimizu","doi":"10.3164/jcbn.23-111","DOIUrl":"10.3164/jcbn.23-111","url":null,"abstract":"Gastrointestinal bleeding (GIB) is a significant public health concern, predominantly associated with high morbidity. However, there have been no reports investigating the trends of GIB in Japan using nationwide data. This study aims to identify current trends and issues in the management of GIB by assessing Japan's national data. We analyzed National Database sampling data from 2012 to 2019, evaluating annual hospitalization rates for major six types of GIB including hemorrhagic gastric ulcers, duodenal ulcers, esophageal variceal bleeding, colonic diverticular bleeding, ischemic colitis, and rectal ulcers. In this study, hospitalization rates per 100,000 indicated a marked decline in hemorrhagic gastric ulcers, approximately two-thirds from 41.5 to 27.9, whereas rates for colonic diverticular bleeding more than doubled, escalating from 15.1 to 34.0. Ischemic colitis rates increased 1.6 times, from 20.8 to 34.9. In 2017, the hospitalization rate per 100,000 for colonic diverticular bleeding and ischemic colitis surpassed those for hemorrhagic gastric ulcers (31.1, 31.3, and 31.0, respectively). No significant changes were observed for duodenal ulcers, esophageal variceal bleeding, or rectal ulcers. The findings of this study underscore a pivotal shift in hospitalization frequencies from upper GIB to lower GIB in 2017, indicating a potential shift in clinical focus and resource allocation.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"75 1","pages":"60-64"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11273266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suppressive effect of resveratrol, catechin and their conformationally constrained analogs on neutrophil extracellular trap formation by HL-60-derived neutrophils. 白藜芦醇、儿茶素及其构象约束类似物对源自 HL-60 的中性粒细胞形成中性粒细胞胞外陷阱的抑制作用

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2024-07-01 Epub Date: 2024-03-05 DOI: 10.3164/jcbn.23-80

Hitomi Ohinata, Wiraphol Phimarn, Mirei Mizuno, Takashi Obama, Kiyoshi Fukuhara, Tomohiko Makiyama, Yuichi Watanabe, Hiroyuki Itabe

{"title":"Suppressive effect of resveratrol, catechin and their conformationally constrained analogs on neutrophil extracellular trap formation by HL-60-derived neutrophils.","authors":"Hitomi Ohinata, Wiraphol Phimarn, Mirei Mizuno, Takashi Obama, Kiyoshi Fukuhara, Tomohiko Makiyama, Yuichi Watanabe, Hiroyuki Itabe","doi":"10.3164/jcbn.23-80","DOIUrl":"10.3164/jcbn.23-80","url":null,"abstract":"Neutrophil extracellular trap (NET) formation is a unique self-defense mechanism of neutrophils; however, it is also involved in many diseases, including atherosclerosis. Resveratrol and catechin are antioxidants with anti-atherosclerotic properties. Here, we examined the effects of resveratrol, catechin, and other related compounds on NET formation. HL-60-derived neutrophils were pretreated with resveratrol and other compounds before stimulation with phorbol-myristate acetate (PMA). DNA and myeloperoxidase released from neutrophils were determined. Resveratrol suppressed the DNA release from neutrophils in a dose-dependent manner. NET formation was enhanced by 1-palmitoyl-2-oxovaleroyl phosphatidylcholine (POVPC), a truncated form of oxidized phospholipid, and resveratrol suppressed NET formation induced by POVPC and PMA. Furthermore, we designed several analogs of resveratrol or catechin whose conformation was restricted by the inhibition of the free rotation of aromatic rings. The conformationally constrained analogs were more effective at inhibiting NET formation; however, their inhibitory function decreased when compound was a large, hydrophobic analog. The most potent compounds, planar catechin and resveratrol, suppressed myeloperoxidase release from activated neutrophils. In addition, these compounds suppressed DNA release from neutrophils stimulated with calcium ionophore. These results suggest that resveratrol, catechin and their analogs exert anti-NET effects, and that constraining the geometry of these compounds enhanced their inhibitory effects.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"75 1","pages":"17-23"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11273275/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of copper and SOD3-mediated extracellular redox regulation in tumor progression. 铜和 SOD3 介导的细胞外氧化还原调节在肿瘤进展中的作用

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2024-07-01 Epub Date: 2024-04-06 DOI: 10.3164/jcbn.24-14

Tetsuro Kamiya

{"title":"Role of copper and SOD3-mediated extracellular redox regulation in tumor progression.","authors":"Tetsuro Kamiya","doi":"10.3164/jcbn.24-14","DOIUrl":"10.3164/jcbn.24-14","url":null,"abstract":"Copper (Cu), an essential micronutrient, participates in several physiological processes, including cell proliferation and development. Notably, the disturbance of Cu homeostasis promotes tumor progression through the generation of oxidative stress. Chronic or excessive accumulation of reactive oxygen species (ROS) causes lipid peroxidation, protein denaturation, and enzyme inactivation, which leads to a breakdown of intracellular homeostasis and exacerbates tumor progression. The disruption of the ROS scavenging mechanism also reduces resistance to oxidative stress, leading to further deterioration in a disease state, and maintenance of redox homeostasis is thought to inhibit the onset and progression of various diseases. Superoxide dismutase 3 (SOD3), a Cu-containing secretory antioxidative enzyme, plays a key role in extracellular redox regulation, and the significant reduction in SOD3 facilitates tumor progression. Furthermore, the significant induction of SOD3 participates in tumor metastasis. This review focuses on the role of Cu homeostasis and antioxidative enzymes, including SOD3, in tumor progression, to help clarify the role of redox regulation.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"75 1","pages":"1-6"},"PeriodicalIF":2.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11273271/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eurycomanone from Eurycoma longifolia Jack upregulates neurotrophin-3 gene expression in retinal Müller cells in vitro. Eurycoma longifolia Jack 中的 Eurycomanone 能上调体外视网膜 Müller 细胞中神经营养素-3 基因的表达。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2024-05-01 Epub Date: 2023-12-15 DOI: 10.3164/jcbn.23-73

Yumi Sakai, Masayoshi Yamada, Tomomichi Watanabe, Arisa Yamazaki, Megumi Furukawa, Nobuo Izumo, Hideo Matsuzaki

{"title":"Eurycomanone from Eurycoma longifolia Jack upregulates neurotrophin-3 gene expression in retinal Müller cells in vitro.","authors":"Yumi Sakai, Masayoshi Yamada, Tomomichi Watanabe, Arisa Yamazaki, Megumi Furukawa, Nobuo Izumo, Hideo Matsuzaki","doi":"10.3164/jcbn.23-73","DOIUrl":"10.3164/jcbn.23-73","url":null,"abstract":"Photoreceptor degeneration decreases light sensitivity and leads to vision loss and various retinal diseases. Neurotrophin-3, originating from Müller glial cells in the retina, plays a key role in protecting photoreceptors from damage induced by light or hypoxia. This neuroprotective approach is important because there are no established methods to regenerate lost photoreceptors. Dietary supplements are one of the useful methods for improving eye health. Eurycoma longifolia (E. longifolia) Jack, which is native to the tropical forest of Malaysia and other Southeast Asian countries, exhibits several medicinal properties. In the present study, we demonstrated that the water extract of E. longifolia roots enhanced neurotrophin-3 gene expression in primary rat Müller cells. Using a stepwise bioassay-guided fractionation and purification of E. longifolia root extracts, we isolated the active compound underlying neurotrophin-3 gene-enhancing activities. Mass spectrometry and nuclear magnetic resonance spectral data identified the compound as eurycomanone. This study provides evidence for the efficacy of E. longifolia and eurycomanone in enhancing neurotrophin-3 expression in Müller cells in vitro. Although the biological significance of this effect and its underlying mechanism remain to be elucidated, this study suggests that E. longifolia may be promising for improving eye health and must be further investigated.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"74 3","pages":"199-206"},"PeriodicalIF":2.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11111470/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141154439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the relationship between colonoscopy insertion difficulty factors and endoscope shape using an endoscopic position detection unit. 使用内窥镜位置检测装置研究结肠镜插入难度因素与内窥镜形状之间的关系。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2024-05-01 Epub Date: 2023-12-15 DOI: 10.3164/jcbn.23-109

Takashi Kawai, Yusuke Kawai, Yoshika Akimito, Mariko Hamada, Eri Iwata, Ryota Niikura, Naoyoshi Nagata, Mitsushige Sugimoto, Kyosuke Yanagisawa, Masakatsu Fukuzawa, Takao Itoi

{"title":"Investigation of the relationship between colonoscopy insertion difficulty factors and endoscope shape using an endoscopic position detection unit.","authors":"Takashi Kawai, Yusuke Kawai, Yoshika Akimito, Mariko Hamada, Eri Iwata, Ryota Niikura, Naoyoshi Nagata, Mitsushige Sugimoto, Kyosuke Yanagisawa, Masakatsu Fukuzawa, Takao Itoi","doi":"10.3164/jcbn.23-109","DOIUrl":"10.3164/jcbn.23-109","url":null,"abstract":"In this study, we investigated the relationship between the cecal intubation time (CIT) and the form and method used for passing through the sigmoid/descending colon junction (SDJ) and the hepatic flexure using an endoscopic position detection unit (UPD), with reference to various factors [age, sex, body mass index (BMI), history of abdominal and pelvic surgery, and diverticulum]. A total of 152 patients underwent colonoscopy with UPD. The mean age was 66.9 ± 12.4 years, and the male to female ratio was 3.6:1. The average CIT time was 14.3 ± 8.2 min. Age, number of experienced endoscopies, history of abdominal and pelvic surgery, BMI, and diverticulum were associated with prolonged CIT; SDJ passage pattern was straight: 8.6 ± 5.0, alpha loop: 11.8 ± 5.6, puzzle ring-like loop: 20.2 ± 5.0, reverse alpha loop: 22.4 ± 9.7, and other loop: 24.7 ± 10.5. The hepatic flexure passing method was in the following order: right rotation maneuver: 12.6 ± 6.6, push maneuver: 15.1 ± 5.9, and right rotation with positional change maneuver: 20.5 ± 7.2. In conclusion, colonoscopy with UPD revealed an association between CIT and SDJ passage pattern and hepatic flexure passing method.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"74 3","pages":"245-252"},"PeriodicalIF":2.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11111465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141154484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sirtuin 3 ameliorates inflammatory bowel disease via inhibiting intestinal inflammation and oxidative stress. Sirtuin 3 可通过抑制肠道炎症和氧化应激改善炎症性肠病。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2024-05-01 Epub Date: 2024-02-09 DOI: 10.3164/jcbn.23-42

Zhen Qin, Qiang-Qiang Chu, An-Lan Ding, Chuan-Ying Li, Mao-Yan Zhang

{"title":"Sirtuin 3 ameliorates inflammatory bowel disease via inhibiting intestinal inflammation and oxidative stress.","authors":"Zhen Qin, Qiang-Qiang Chu, An-Lan Ding, Chuan-Ying Li, Mao-Yan Zhang","doi":"10.3164/jcbn.23-42","DOIUrl":"10.3164/jcbn.23-42","url":null,"abstract":"Sirtuin 3 involved in development of various diseases, but its role in inflammatory bowel disease is still unknown. We used inflammatory bowel disease biopsies, colitis animal model, and vitro cells RAW264.7 to study the role of Sirtuin 3 in the pathophysiology of inflammatory bowel disease. Sirtuin 3 negatively correlated with intestinal TNF-α. Sirt3 was less pronounced in pediatric and adult inflammatory bowel disease patients compared with corresponding control group. Sirtuin 3 activator Honokiol suppressed dextran sulfate sodium induced colonic manifestations, while Sirt3 inhibitor caused opposite results. Honokiol inhibited colonic oxidative stress by and reduced intestinal permeability. Honokiol repressed inflammatory response by reducing macrophage infiltration, pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 levels, and inhibiting activation of NF-κB p65 in the colitis mice. However, Sirt3 inhibitor amplified colonic oxidative stress and inflammatory response. In vitro study, Sirt3 inhibitor or siRNA Sirtuin 3 activated NF-κB p65 and enhanced TNF-α, IL-1β, and IL-6 secretion from LPS stimulated RAW264.7, while Honokiol remarkably attenuated these pro-inflammatory cytokines secretion. Finally, knockdown of Sirt3 in Caco-2 cells enhanced TNF-α induced intestinal barrier integrity injury. Sirtuin 3 negatively regulates inflammatory bowel disease progression via reducing colonic inflammation and oxidative stress. Sirtuin 3 is a promising therapeutic target in clinical application for inflammatory bowel disease therapy.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"74 3","pages":"235-244"},"PeriodicalIF":2.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11111467/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141154665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reactive oxygen species induced by indomethacin enhance accumulation of heme carrier protein 1 and hematoporphyrin accumulation in vitro and in vivo in a brain tumor model. 吲哚美辛诱导的活性氧可增强脑肿瘤模型中血红素载体蛋白1的体外和体内蓄积以及血卟啉的蓄积。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2024-05-01 Epub Date: 2024-02-01 DOI: 10.3164/jcbn.23-20

Hidehiro Kohzuki, Hiromu Ito, Hiromi Kurokawa, Hirofumi Matsui, Tetsuya Yamamoto, Eiichi Ishikawa

{"title":"Reactive oxygen species induced by indomethacin enhance accumulation of heme carrier protein 1 and hematoporphyrin accumulation in vitro and in vivo in a brain tumor model.","authors":"Hidehiro Kohzuki, Hiromu Ito, Hiromi Kurokawa, Hirofumi Matsui, Tetsuya Yamamoto, Eiichi Ishikawa","doi":"10.3164/jcbn.23-20","DOIUrl":"10.3164/jcbn.23-20","url":null,"abstract":"Photodynamic therapy (PDT) is useful for various cancers such as high-grade glioma and cancers of other organs. However, the mechanism of tumor-specific accumulation of porphyrin is not clear. The authors previously reported that heme carrier protein 1 (HCP1) contributes to the transport of porphyrins; specifically, we showed that the production of cancer-specific reactive oxygen species from mitochondria (mitROS) leads in turn to enhanced HCP1 expression. Indomethacin (IND), a non-steroidal anti-inflammatory drug, increases ROS production by affecting mitochondrial electron transfer system. In the present work, the authors investigated the effect of pretreatment with IND on cancer-specific porphyrin accumulation, using both a glioma cell line and a rat brain tumor model. This work demonstrated that exposure of a rat glioma cell to IND results in increased generation of cancer-specific mitROS and accumulation of HCP1 expression and porphyrin concentration. Additionally, systemic dosing of a brain tumor animal model with IND resulted in elevated cellular accumulation of porphyrin in tumor cell. This is an effect not seen with normal brain tissue. Thus, the administration of IND increases intracellular porphyrin concentrations in tumor cell without exerting harmful effects on normal brain tissue, and increased porphyrin concentration in tumor cell may lead to improved PDT effect.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"74 3","pages":"207-212"},"PeriodicalIF":2.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11111468/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141156176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crosstalk between oxygen signaling and iron metabolism in renal interstitial fibroblasts. 肾间质成纤维细胞中氧信号传导与铁代谢之间的相互关系

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2024-05-01 Epub Date: 2024-02-28 DOI: 10.3164/jcbn.24-8

Norio Suzuki, Yuma Iwamura, Koichiro Kato, Hirotaka Ishioka, Yusuke Konta, Koji Sato, Nao Uchida, Noa Koida, Hiroki Sekine, Tetsuhiro Tanaka, Naonori Kumagai, Taku Nakai

{"title":"Crosstalk between oxygen signaling and iron metabolism in renal interstitial fibroblasts.","authors":"Norio Suzuki, Yuma Iwamura, Koichiro Kato, Hirotaka Ishioka, Yusuke Konta, Koji Sato, Nao Uchida, Noa Koida, Hiroki Sekine, Tetsuhiro Tanaka, Naonori Kumagai, Taku Nakai","doi":"10.3164/jcbn.24-8","DOIUrl":"10.3164/jcbn.24-8","url":null,"abstract":"To maintain the oxygen supply, the production of red blood cells (erythrocytes) is promoted under low-oxygen conditions (hypoxia). Oxygen is carried by hemoglobin in erythrocytes, in which the majority of the essential element iron in the body is contained. Because iron metabolism is strictly controlled in a semi-closed recycling system to protect cells from oxidative stress caused by iron, hypoxia-inducible erythropoiesis is closely coordinated by regulatory systems that mobilize stored iron for hemoglobin synthesis. The erythroid growth factor erythropoietin (EPO) is mainly secreted by interstitial fibroblasts in the renal cortex, which are known as renal EPO-producing (REP) cells, and promotes erythropoiesis and iron mobilization. Intriguingly, EPO production is strongly induced by hypoxia through iron-dependent pathways in REP cells. Here, we summarize recent studies on the network mechanisms linking hypoxia-inducible EPO production, erythropoiesis and iron metabolism. Additionally, we introduce disease mechanisms related to disorders in the network mediated by REP cell functions. Furthermore, we propose future studies regarding the application of renal cells derived from the urine of kidney disease patients to investigate the molecular pathology of chronic kidney disease and develop precise and personalized medicine for kidney disease.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"74 3","pages":"179-184"},"PeriodicalIF":2.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11111471/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141154200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0