Journal of Clinical Biochemistry and Nutrition最新文献_第9页

Relationships between body mass index and constipation, gastroesophageal reflux disease, stool forms based on the Bristol Stool Form Scale, and education level: results from an internet survey in Japan. 体质指数与便秘、胃食管反流病、基于布里斯托大便形式量表的大便形式和教育水平之间的关系:来自日本一项互联网调查的结果。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-07-01 DOI: 10.3164/jcbn.22-143

Naotaka Ogasawara, Kunio Kasugai, Yasushi Funaki, Masahide Ebi, Shinya Izawa, Yasuhiro Tamura, Aya Kato, Yoshiharu Yamaguchi, Kazunori Adachi, Tomoya Sugiyama, Makoto Sasaki

{"title":"Relationships between body mass index and constipation, gastroesophageal reflux disease, stool forms based on the Bristol Stool Form Scale, and education level: results from an internet survey in Japan.","authors":"Naotaka Ogasawara, Kunio Kasugai, Yasushi Funaki, Masahide Ebi, Shinya Izawa, Yasuhiro Tamura, Aya Kato, Yoshiharu Yamaguchi, Kazunori Adachi, Tomoya Sugiyama, Makoto Sasaki","doi":"10.3164/jcbn.22-143","DOIUrl":"https://doi.org/10.3164/jcbn.22-143","url":null,"abstract":"Detailed evaluations of body mass index (BMI) and stool form based on the Bristol Stool Form Scale (BSFS) in individuals with constipation, gastroesophageal reflux disease (GERD), and concomitant constipation and GERD have not been performed in Japan. This study was an internet survey conducted to examine the relationships between BMI and constipation, GERD, stool forms based on the BSFS, and education level. This internet-based survey recruited participants from general public survey panels. 10,000 individuals meeting the eligibility criteria were enrolled. Questions included demographics, medical data, and assessments based on validated measures for constipation and GERD. BMI was significantly lower in males with versus without constipation. BMI was significantly higher with GERD both males and females. Mean BMI increased from the BSFS-1/2 group through the BSFS-3/4/5 to the BSFS-6/7 groups in both sexes. BMI was highest in individuals with a maximum education level of junior high school and second highest in individuals completing high school. This is the first real-world survey that closely examines the relationship between BMI and stool forms of individuals in Japan. When the BMI increased, stool forms varied from hard to watery in Japanese people. BMI was related with education level in Japan. (Trial registration: UMIN000039688).","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"73 1","pages":"84-90"},"PeriodicalIF":2.4,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9d/b7/jcbn22-143.PMC10390807.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9933509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The alteration of serum bile acid profile among traumatic brain injury patients: a small-scale prospective study. 外伤性脑损伤患者血清胆汁酸谱的改变:一项小规模前瞻性研究。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-07-01 DOI: 10.3164/jcbn.23-10

Yuanrun Zhu, Peidong Zheng, Yajun Lin, Juehan Wang, Wendong You, Yadong Wang, Huiqing Zheng, Liang Wen, Xiaofeng Yang

{"title":"The alteration of serum bile acid profile among traumatic brain injury patients: a small-scale prospective study.","authors":"Yuanrun Zhu, Peidong Zheng, Yajun Lin, Juehan Wang, Wendong You, Yadong Wang, Huiqing Zheng, Liang Wen, Xiaofeng Yang","doi":"10.3164/jcbn.23-10","DOIUrl":"https://doi.org/10.3164/jcbn.23-10","url":null,"abstract":"Traumatic brain injury is one of the major causes of morbidity and mortality worldwide. With the development of bile acids as a potential treatment, to identify the influence of traumatic brain injury on bile acid metabolism shows growing importance. This present study did a preliminary exploration of the bile acid profile alteration among traumatic brain injury patients. In total, 14 patients and 7 healthy volunteers were enrolled. The bile acid profile of the blood samples were detected by an Ultra-performance Liquid Chromatography Mass Spectrometer/Mass Spectrometer system. It was found that 6 bile acids were statistically decreased in traumatic brain injury patients comparing with healthy volunteers: glycocholic acid (median level 44.4 ng/ml vs 98.7 ng/ml, p = 0.003), taurocholic acid (median level 10.9 ng/ml vs 19.5 ng/ml, p = 0.006), glycoursodeoxycholic acid (median level 17.4 ng/ml vs 71.4 ng/ml, p = 0.001), ursodeoxycholic acid (median level <1 ng/ml vs 32.4 ng/ml, p = 0.002), taurochenodeoxycholic acid (median level <1 ng/ml vs 53.6 ng/ml, p = 0.003) and glycochenodeoxycholic acid (GCDCA, median level 160 ng/ml vs 364 ng/ml, p<0.001). In conclusion, traumatic brain injury events are able to induce bile acid metabolism alteration in plasma and might cause reduction in glycocholic, taurocholic, glycoursodeoxycholic, ursodeoxycholic, taurochenodeoxycholic and glycochenodeoxycholic acid levels.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"73 1","pages":"97-102"},"PeriodicalIF":2.4,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/80/eb/jcbn23-10.PMC10390815.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9924395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-fat diet causes mitochondrial damage and downregulation of mitofusin-2 and optic atrophy-1 in multiple organs. 高脂肪饮食导致多器官线粒体损伤、mitofusin-2下调和视神经萎缩-1。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-07-01 DOI: 10.3164/jcbn.22-73

Peng Zheng, Wenjing Ma, Yilu Gu, Hengfang Wu, Zhiping Bian, Nannan Liu, Di Yang, Xiangjian Chen

{"title":"High-fat diet causes mitochondrial damage and downregulation of mitofusin-2 and optic atrophy-1 in multiple organs.","authors":"Peng Zheng, Wenjing Ma, Yilu Gu, Hengfang Wu, Zhiping Bian, Nannan Liu, Di Yang, Xiangjian Chen","doi":"10.3164/jcbn.22-73","DOIUrl":"https://doi.org/10.3164/jcbn.22-73","url":null,"abstract":"High-fat consumption promotes the development of obesity, which is associated with various chronic illnesses. Mitochondria are the energy factories of eukaryotic cells, maintaining self-stability through a fine-tuned quality-control network. In the present study, we evaluated high-fat diet (HFD)-induced changes in mitochondrial ultrastructure and dynamics protein expression in multiple organs. C57BL/6J male mice were fed HFD or normal diet (ND) for 24 weeks. Compared with ND-fed mice, HFD-fed mice exhibited increased body weight, cardiomyocyte enlargement, pulmonary fibrosis, hepatic steatosis, renal and splenic structural abnormalities. The cellular apoptosis of the heart, liver, and kidney increased. Cellular lipid droplet deposition and mitochondrial deformations were observed. The proteins related to mitochondrial biogenesis (TFAM), fission (DRP1), autophagy (LC3 and LC3-II: LC3-I ratio), and mitophagy (PINK1) presented different changes in different organs. The mitochondrial fusion regulators mitofusin-2 (MFN2) and optic atrophy-1 (OPA1) were consistently downregulated in multiple organs, even the spleen. TOMM20 and ATP5A protein were enhanced in the heart, skeletal muscle, and spleen, and attenuated in the kidney. These results indicated that high-fat feeding caused pathological changes in multiple organs, accompanied by mitochondrial ultrastructural damage, and MFN2 and OPA1 downregulation. The mitochondrial fusion proteins may become promising targets and/or markers for treating metabolic disease.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"73 1","pages":"61-76"},"PeriodicalIF":2.4,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d8/fe/jcbn22-73.PMC10390808.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9924399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quenching effect of cerium oxide nanoparticles on singlet oxygen: validation of the potential for reaction with multiple reactive oxygen species. 氧化铈纳米粒子对单线态氧的猝灭效应:与多种活性氧反应电位的验证。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-07-01 DOI: 10.3164/jcbn.22-68

Yukihiro Ogawa, Tsunetaka Kawaguchi, Mami Tanaka, Akiko Hashimoto, Koji Fukui, Naofumi Uekawa, Toshihiko Ozawa, Toshiaki Kamachi, Masahiro Kohno

{"title":"Quenching effect of cerium oxide nanoparticles on singlet oxygen: validation of the potential for reaction with multiple reactive oxygen species.","authors":"Yukihiro Ogawa, Tsunetaka Kawaguchi, Mami Tanaka, Akiko Hashimoto, Koji Fukui, Naofumi Uekawa, Toshihiko Ozawa, Toshiaki Kamachi, Masahiro Kohno","doi":"10.3164/jcbn.22-68","DOIUrl":"https://doi.org/10.3164/jcbn.22-68","url":null,"abstract":"Here we studied cerium oxide nanoparticles (nanoceria) as an agent for the future treatment of oxidative damage by validating and evaluating its scavenging activity towards reactive oxygen species (ROS) in vitro. Nanoceria has been shown to mimic the activities of superoxide dismutase and catalase, degrading superoxide (O2•-) and hydrogen peroxide (H2O2). We examined the antioxidative activity of nanoceria, focusing on its ability to quench singlet oxygen (1O2) in an aqueous solution. Electron paramagnetic resonance (EPR) was used to determine the rates of second-order reactions between nanoceria and three ROS (1O2, O2•-, and H2O2) in aqueous solution, and its antioxidative abilities were demonstrated. Nanoceria shows a wide range of ultraviolet-light absorption bands and thus 1O2 was produced directly in a nanoceria suspension using high-frequency ultrasound. The quenching or scavenging abilities of nanoceria for 1O2 and hypoxanthine-xanthine oxidase reaction-derived O2•- were examined by EPR spin-trapping methods, and the consumption of H2O2 was estimated by the EPR oximetry method. Our results indicated that nanoceria interact not only with two previously reported ROS but also with 1O2. Nanoceria were shown to degrade O2•- and H2O2, and their ability to quench 1O2 may be one mechanism by which they protect against oxidative damage such as inflammation.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"73 1","pages":"1-8"},"PeriodicalIF":2.4,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/14/6c/jcbn22-68.PMC10390806.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9924396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Oxidative stress induces tau hyperphosphorylation via MARK activation in neuroblastoma N1E-115 cells. 在神经母细胞瘤N1E-115细胞中，氧化应激通过MARK激活诱导tau过度磷酸化。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-07-01 DOI: 10.3164/jcbn.22-39

Yuhong Liu, Yunxi Chen, Koji Fukui

{"title":"Oxidative stress induces tau hyperphosphorylation via MARK activation in neuroblastoma N1E-115 cells.","authors":"Yuhong Liu, Yunxi Chen, Koji Fukui","doi":"10.3164/jcbn.22-39","DOIUrl":"https://doi.org/10.3164/jcbn.22-39","url":null,"abstract":"Reactive oxygen species are considered a cause of neuronal cell death in Alzheimer's disease (AD). Abnormal tau phosphorylation is a proven pathological hallmark of AD. Microtubule affinity-regulating kinases (MARKs) regulate tau-microtubule binding and play a crucial role in neuronal survival. In this study, we hypothesized that oxidative stress increases the phosphorylation of Ser262 of tau protein through activation of MARKs, which is the main reason for the development of AD. We investigated the relationship between tau hyperphosphorylation on Ser262 and MARKs in N1E-115 cells subjected to oxidative stress by exposure to a low concentration of hydrogen peroxide. This work builds on the observation that hyperphosphorylation of tau is significantly increased by oxidative stress. MARKs activation correlated with tau hyperphosphorylation at Ser262, a site that is essential to maintain microtubule stability and is the initial phosphorylation site in AD. These results indicated that MARKs inhibitors might serve a role as therapeutic tools for the treatment of AD.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"73 1","pages":"24-33"},"PeriodicalIF":2.4,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9a/4c/jcbn22-39.PMC10390814.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9935910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitophagy-mediated inflammation and oxidative stress contribute to muscle wasting in cancer cachexia. 线粒体介导的炎症和氧化应激有助于癌症恶病质中的肌肉萎缩。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-07-01 DOI: 10.3164/jcbn.23-1

Zhige Zhang, Shanjun Tan, Shuhao Li, Yuxi Cheng, Junjie Wang, Hao Liu, Mingyue Yan, Guohao Wu

{"title":"Mitophagy-mediated inflammation and oxidative stress contribute to muscle wasting in cancer cachexia.","authors":"Zhige Zhang, Shanjun Tan, Shuhao Li, Yuxi Cheng, Junjie Wang, Hao Liu, Mingyue Yan, Guohao Wu","doi":"10.3164/jcbn.23-1","DOIUrl":"https://doi.org/10.3164/jcbn.23-1","url":null,"abstract":"Cancer cachexia is commonly seen in patients with malignant tumors, which usually leads to poor life quality and negatively affects long-term prognosis and survival. Mitochondria dysfunction and enhanced autophagy are well-established to play an important role in skeletal muscle wasting. However, whether mitophagy is engaged in the pathogenesis of cancer cachexia requires further investigation. This study comprised a clinical study and animal experimentation. Clinical data such as CT images and laboratory results were obtained and analyzed. Then mice model of cancer cachexia and mitophagy inhibition were established. Data including skeletal muscle mass and function, mitochondria structure and function, inflammatory factors as well as ROS concentration. Mitophagy was enhanced in cancer cachexia patients with increased inflammatory factors. Greater disruption of skeletal muscle fiber and mitochondria structure were seen in cancer cachexia, with a higher level of inflammatory factors and ROS expression in skeletal muscle. Meanwhile, ATP production was undermined, indicating a close relationship with mitophagy, inflammation, and oxidative stress in the skeletal muscle of cancer cachexia mice models. In conclusion, mitophagy is activated in cancer cachexia and may play a role in skeletal muscle atrophy, and inflammation and oxidative stress might participate in mitophagy-related skeletal muscle injury.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"73 1","pages":"34-42"},"PeriodicalIF":2.4,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b1/3e/jcbn23-1.PMC10390805.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9924397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GPRC5A regulates proliferation and oxidative stress by inhibiting the STAT3/Socs3/c-MYC pathway in hepatocellular carcinoma. GPRC5A通过抑制肝细胞癌STAT3/Socs3/c-MYC通路调控增殖和氧化应激。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-07-01 DOI: 10.3164/jcbn.22-125

Lixia Zhang, Weibing Yang, Jin Yang, Fu Sun

{"title":"GPRC5A regulates proliferation and oxidative stress by inhibiting the STAT3/Socs3/c-MYC pathway in hepatocellular carcinoma.","authors":"Lixia Zhang, Weibing Yang, Jin Yang, Fu Sun","doi":"10.3164/jcbn.22-125","DOIUrl":"https://doi.org/10.3164/jcbn.22-125","url":null,"abstract":"The G protein-coupled receptor, class C, group 5, member A (GPRC5A) plays a key role in various diseases, but its effect on hepatocellular carcinoma (HCC) and the potential underlying mechanisms remains unclear. In the present study, we explored the effect of GPRC5A on the progression of HCC and further explored its mechanism of action. The results revealed that the expression of GPRC5A was lower in HCC tissues and cells. Overexpression of GPRC5A suppressed the proliferation and epithelial-mesenchymal transition (EMT) of HCC cells. In addition, overexpression of GPRC5A induced oxidative stress and apoptosis. Further study showed that overexpression of GPRC5A inhibited the expression of STAT3/Socs3/c-MYC related-protein and the NLRP3 inflammasome. Moreover, the STAT3/Socs3/c-MYC and NLRP3 inflammasome was involved in the effect of GPRC5A on HCC cells. These results suggest that GPRC5A suppresses proliferation and EMT, induces oxidative stress and leads to apoptosis of HCC cells, potentially by regulating STAT3/Socs3/c-MYC signalling and the NLRP3 inflammasome. These findings suggest that GPRC5A has an anti-tumor effect in the formation of HCC, and the molecular therapy of GPRC5A provides a theoretical basis for treating HCC.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"73 1","pages":"43-51"},"PeriodicalIF":2.4,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/49/d0/jcbn22-125.PMC10390809.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9930371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reduced abundance of butyric acid-producing bacteria in the ileal mucosa-associated microbiota of ulcerative colitis patients. 溃疡性结肠炎患者回肠粘膜相关微生物群中产丁酸的细菌数量减少。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-07-01 Epub Date: 2023-04-14 DOI: 10.3164/jcbn.22-86

Motoyasu Osawa, Osamu Handa, Shinya Fukushima, Hiroshi Matsumoto, Eiji Umegaki, Ryo Inoue, Yuji Naito, Akiko Shiotani

{"title":"Reduced abundance of butyric acid-producing bacteria in the ileal mucosa-associated microbiota of ulcerative colitis patients.","authors":"Motoyasu Osawa, Osamu Handa, Shinya Fukushima, Hiroshi Matsumoto, Eiji Umegaki, Ryo Inoue, Yuji Naito, Akiko Shiotani","doi":"10.3164/jcbn.22-86","DOIUrl":"10.3164/jcbn.22-86","url":null,"abstract":"Compositional changes in the microbiota are associated with various inflammatory diseases, including ulcerative colitis (UC). Aim: This study aimed to investigate the mucosa-associated microbiota (MAM) in patients with UC and its difference related with disease activity and classification. Brush samples were collected from the terminal ileum and sigmoid colon during endoscopic procedures. The microbiota of samples was profiled using the Illumina MiSeq platform. The V3-V4 regions of the gene encoding 16S rRNA (460 bp) were amplified using PCR. Fifty UC patients and twenty healthy controls were enrolled. UC patients displayed significantly reduced α-diversity in both the ileum and sigmoid colon compared to controls. A difference in β-diversity in the unweighted analysis was observed between the two groups. The abundance of Lactobacillus and Veillonella was significantly higher and that of Butyricicoccus, Ruminococcus and Lachnospiraceae was significantly lower in the ileum of UC patients than in controls. The abundance of Odoribacter in the ileum was significantly lower in left-sided colitis and pancolitis patients than in proctitis patients and lower in patients with highly severe disease activity than with mild disease activity. The reduction in abundance of butyric acid-producing bacteria, especially Odoribacter, in ileal MAM may play an important role in the pathophysiology of UC.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"73 1","pages":"77-83"},"PeriodicalIF":2.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/99/29/jcbn22-86.PMC10390811.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9930373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The dose-response relationships of the direct scavenging activity of amide-based local anesthetics against multiple free radicals. 酰胺类局部麻醉剂对多种自由基的直接清除活性的量效关系。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-07-01 DOI: 10.3164/jcbn.22-131

Yukiko Sato, Shigekiyo Matsumoto, Kazue Ogata, Kira Bacal, Misato Nakatake, Takaaki Kitano, Osamu Tokumaru

{"title":"The dose-response relationships of the direct scavenging activity of amide-based local anesthetics against multiple free radicals.","authors":"Yukiko Sato, Shigekiyo Matsumoto, Kazue Ogata, Kira Bacal, Misato Nakatake, Takaaki Kitano, Osamu Tokumaru","doi":"10.3164/jcbn.22-131","DOIUrl":"https://doi.org/10.3164/jcbn.22-131","url":null,"abstract":"This study aimed to illustrate the dose-response relationships of the direct scavenging activity of amide-based local anesthetics against multiple free radicals in vitro. We have demonstrated that amide-type local anesthetics selectively and directly scavenge some free radicals. Three kinds of free radicals were eliminated by all the four local anesthetics examined. Mepivacaine, lidocaine, bupivacaine, and dibucaine scavenged hydroxyl radicals in dose-dependent manners. Ascorbyl free radicals were also scavenged in dose-dependent manners, and lastly singlet oxygen was scavenged in dose-dependent manners. Three other free radicals were not scavenged by all of the four local anesthetics; tert-butoxyl radical was scavenged by all the anesthetics examined but dibucaine, nitric oxide by mepivacaine but not by the other three, and tyrosyl radical by mepivacaine and lidocaine but not by the other two. Some free radicals (superoxide anion, tert-butyl peroxyl radical, DPPH) were not scavenged by any of the four local anesthetics. The local anesthetics were also shown to inhibit lipid peroxidation by TBARS assay. These results suggest that local anesthetics have antioxidant properties through their free radical scavenging activities.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"73 1","pages":"16-23"},"PeriodicalIF":2.4,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/bd/c5/jcbn22-131.PMC10390804.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9930374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of luseogliflozin treatment on hyperglycemia-induced muscle atrophy in rats. 糖格列净对高血糖所致大鼠肌肉萎缩的影响。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-05-01 DOI: 10.3164/jcbn.22-58

Keyu Xie, Ken Sugimoto, Minoru Tanaka, Hiroshi Akasaka, Taku Fujimoto, Toshimasa Takahashi, Yuri Onishi, Tomohiro Minami, Shino Yoshida, Yoichi Takami, Koichi Yamamoto, Hiromi Rakugi

{"title":"Effects of luseogliflozin treatment on hyperglycemia-induced muscle atrophy in rats.","authors":"Keyu Xie, Ken Sugimoto, Minoru Tanaka, Hiroshi Akasaka, Taku Fujimoto, Toshimasa Takahashi, Yuri Onishi, Tomohiro Minami, Shino Yoshida, Yoichi Takami, Koichi Yamamoto, Hiromi Rakugi","doi":"10.3164/jcbn.22-58","DOIUrl":"https://doi.org/10.3164/jcbn.22-58","url":null,"abstract":"Diabetes mellitus is recognized as a risk factor for sarcopenia. Luseogliflozin, a selective sodium-glucose cotransporter 2 (SGLT2) inhibitor, reduces inflammation and oxidative stress by improving hyperglycemia, subsequently improving hepatosteatosis or kidney dysfunction. However, the effects of SGLT2 inhibitor on the regulation of skeletal muscle mass or function in hyperglycemia are still unknown. In this study, we investigated the effects of luseogliflozin-mediated attenuation of hyperglycemia on the prevention of muscle atrophy. Twenty-four male Sprague-Dawley rats were randomly divided into four groups: control, control with SGLT2 inhibitor treatment, hyperglycemia, and hyperglycemia with SGLT2 inhibitor treatment. The hyperglycemic rodent model was established using a single injection of streptozotocin, a compound with preferential toxicity toward pancreatic beta cells. Muscle atrophy in streptozotocin-induced hyperglycemic model rats was inhibited by the suppression of hyperglycemia using luseogliflozin, which consequently suppressed hyperglycemia-mediated increase in the levels of advanced glycation end products (AGEs) and activated the protein degradation pathway in muscle cells. Treatment with luseogliflozin can restore the hyperglycemia-induced loss in the muscle mass to some degree partly through the inhibition of AGEs-induced or homeostatic disruption of mitochondria-induced activation of muscle degradation.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"72 3","pages":"248-255"},"PeriodicalIF":2.4,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6d/ac/jcbn22-58.PMC10209601.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9539747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0