Journal of Clinical Biochemistry and Nutrition最新文献_第8页

Tumor-derived exosomes containing circ_0008039 promote the stemness of colorectal cancer cells by inhibiting ferroptosis. 含有circ_0008039的肿瘤源性外泌体通过抑制铁下垂促进结直肠癌细胞的干性。

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-09-01 Epub Date: 2025-04-11 DOI: 10.3164/jcbn.24-240

Mengjun Shen, Shenghong Wan, Xianwei Yang, Xiaoling Liu, Lin Ma, Xin Liao

{"title":"Tumor-derived exosomes containing circ_0008039 promote the stemness of colorectal cancer cells by inhibiting ferroptosis.","authors":"Mengjun Shen, Shenghong Wan, Xianwei Yang, Xiaoling Liu, Lin Ma, Xin Liao","doi":"10.3164/jcbn.24-240","DOIUrl":"10.3164/jcbn.24-240","url":null,"abstract":"This study was to investigate the impact of exosomes-derived circ_0008039 on ferroptosis and stemness in colorectal cancer cells, as well as its associated molecular mechanisms. The cell colony formation ability was evaluated using the MTT assay and colony formation assay, respectively. Additionally, the sphere formation assay was employed to examine the cells' ability to aggregate into spheres. The targeting relationship between circ_0008039, miR-302e, and SLC7A11 was verified through dual luciferase assay. Furthermore, RNA immunoprecipitation (RIP) analysis of circ_0008039 and miR-302e was conducted. Western blotting was utilized to detect the protein expression of exosome surface antigens (TSG101 and CD63), stem cell markers (CD133, Nanog, and SOX-2), as well as SLC7A11 in both exosomes and cell lysates. The levels of glutathione (GSH), malondialdehyde (MDA), reactive oxygen species (ROS), and Fe2+ were determined using corresponding kits. Circ_0008039 exhibited significant downregulation in cancer tissues and cells. Furthermore, exosome-derived circ_0008039 played a crucial role in promoting cell viability, clone formation, and resistance to ferroptosis by inhibiting MDA, ROS, and Fe2+ levels. Additionally, circ_0008039 acted as a miR-302e sponge to regulate SLC7A11 expression. In colorectal cancer (CRC) tissues, miR-302e was negatively correlated with SLC7A11 expression while circ_0008039 was negatively correlated with miR-302e expression. Mechanistic validation demonstrated that circ_0008039 regulated CRC cell proliferation, stemness maintenance and ferroptosis through the modulation of the miR-302e/SLC7A11 axis. The Circ_0008039/miR-302e/SLC7A11 axis plays a pivotal role in facilitating the proliferation and maintenance of stemness in CRC cells, while enhancing resistance to ferroptosis.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"77 2","pages":"120-130"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12440672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Newly developed evaluation for intestinal permeability using Caenorhabditis elegans: protective effects of agaro-oligosaccharides on intestinal permeability. 用秀丽隐杆线虫评价肠道通透性的新进展：琼脂寡糖对肠道通透性的保护作用。

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-09-01 Epub Date: 2025-03-15 DOI: 10.3164/jcbn.24-149

Natsumi Desaka, Yuji Naito, Yasuki Higashimura

{"title":"Newly developed evaluation for intestinal permeability using Caenorhabditis elegans: protective effects of agaro-oligosaccharides on intestinal permeability.","authors":"Natsumi Desaka, Yuji Naito, Yasuki Higashimura","doi":"10.3164/jcbn.24-149","DOIUrl":"10.3164/jcbn.24-149","url":null,"abstract":"The intestinal barrier represents the first line of host defense. Its dysfunction, defined as increased intestinal permeability, is widely recognized as an important factor in the clinical manifestation of various diseases. Consequently, maintenance of the intestinal barrier is necessary for human health. Caenorhabditis elegans has recently been used frequently as a model organism in studies of gut bacteria-host interactions and in screening for probiotics and prebiotics that promote gut health. Nevertheless, no quantitative method for evaluating the intestinal permeability of Caenorhabditis elegans has yet been established. This study assesses a newly developed evaluation method to assess the intestinal permeability of Caenorhabditis elegans quantitatively using liposomes encapsulating fluorescein isothiocyanate-dextran. The usefulness of this method was confirmed by measuring the intestinal permeability of Pseudomonas aeruginosa-infected or oxidative stress-induced worms. Furthermore, our method found that agaro-oligosaccharides, the hydrolysis products of agarose, have a beneficial function of preventing increased intestinal permeability. This approach can expand the utility of Caenorhabditis elegans for functional food discovery and drug candidate screening, with specific examination of their effects on gut function.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"77 2","pages":"113-119"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12440670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of transarterial chemoembolization on serum opsonic activity in hepatocellular carcinoma measured by chemiluminescence. 化学发光法测定经动脉化疗栓塞对肝癌患者血清opsonic活性的影响。

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-09-01 Epub Date: 2025-05-14 DOI: 10.3164/jcbn.25-80

Satoshi Sato, Daisuke Chinda, Misa Ozaki, Naoki Akimoto, Tetsu Arai, Kenta Yoshida, Chikara Iino, Shinya Kakehata, Fumiyasu Tsushima, Shingo Kakeda, Hirotake Sakuraba

{"title":"Influence of transarterial chemoembolization on serum opsonic activity in hepatocellular carcinoma measured by chemiluminescence.","authors":"Satoshi Sato, Daisuke Chinda, Misa Ozaki, Naoki Akimoto, Tetsu Arai, Kenta Yoshida, Chikara Iino, Shinya Kakehata, Fumiyasu Tsushima, Shingo Kakeda, Hirotake Sakuraba","doi":"10.3164/jcbn.25-80","DOIUrl":"10.3164/jcbn.25-80","url":null,"abstract":"This study aimed to evaluate the physical stress associated with transarterial chemoembolization (TACE), a catheter-based treatment for hepatocellular carcinoma, by examining changes in serum opsonic activity (SOA). SOA was examined by measuring reactive oxygen species (ROS) produced by neutrophils using lucigenin-dependent chemiluminescence (LgCL) and luminol-dependent chemiluminescence (LmCL). Sixty-four patients were enrolled, and SOA was measured at admission, the following day, and 3 days after TACE. The area under the curve (AUC) for LgCL did not change significantly from baseline to the day after TACE but increased significantly from the following day to 3 days post-TACE. In contrast, no changes were observed in the AUC of LmCL. Subgroup analyses revealed a significant increase in LgCL from day 1 to day 3 post-TACE among patients aged >75 years, males, body mass index (BMI) <25 ‍kg/m2, those with a FIB-4 index of ≥2.67, cisplatin use, Hepatitis B virus/Hepatitis C virus-related liver disease, or a procedure time ≥120 ‍min. Multivariate analyses identified BMI <25 ‍kg/m2 and cisplatin use as significant risk factors for increased LgCL. Although TACE is considered a minimally invasive procedure, low BMI and cisplatin use have been identified as notable sources of significant physical stresses.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"77 2","pages":"195-201"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12440674/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial oxidative stress, cellular damages and stem cell aging in premature aging models with complex II electron transport defect. 线粒体氧化应激、细胞损伤和干细胞衰老在复合体II电子传递缺陷早衰模型中的作用。

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-09-01 Epub Date: 2025-07-02 DOI: 10.3164/jcbn.25-62

Takamasa Ishii, Kayo Yasuda, Masaki Miyazawa, Hiromi Onouchi, Sumino Yanase, Naoaki Ishii

{"title":"Mitochondrial oxidative stress, cellular damages and stem cell aging in premature aging models with complex II electron transport defect.","authors":"Takamasa Ishii, Kayo Yasuda, Masaki Miyazawa, Hiromi Onouchi, Sumino Yanase, Naoaki Ishii","doi":"10.3164/jcbn.25-62","DOIUrl":"10.3164/jcbn.25-62","url":null,"abstract":"Mitochondria which are the major intracellular reactive oxygen species (ROS) sources produce especially superoxide anion (O2 •-) as a byproduct of energy production. It has been well known that O2 •- is converted from oxygen (O2) and is overproduced by excessive electron leakage from the mitochondrial electron transport chain (ETC), mainly complexes I and III. However we have previously reported that several point mutations (specifically G71E in C. elegans, I71E in Drosophila and V69E in mouse) in succinate dehydrogenase C subunit (SDHC) of complex II cause mitochondrial electron transport defect leading to O2 •- overproduction from mitochondria. These mutations can cause endogenous oxidative stress resulting in tumorigenesis and apoptosis as well as premature death. Recently, we have also demonstrated that premature aging of hematopoietic stem cell with a mutation in SDHC is developed after the growth phase and normal development. Here, we review cellular damages by complex II electron transport defect-induced endogenous oxidative stress in premature aging models.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"77 2","pages":"101-112"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12440677/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness of the serum ratio of l-cysteine to glutathione for the prediction of cardiovascular events. 血清l-半胱氨酸/谷胱甘肽比值预测心血管事件的有效性。

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-09-01 Epub Date: 2025-05-28 DOI: 10.3164/jcbn.24-156

Yuki Ishinoda, Nobuyuki Masaki, Yasuhiro Hitomi, Ryota Nakazawa, Akira Taruoka, Akane Kawai, Midori Iwashita, Yasuo Ido, Yusuke Yumita, Kazuki Kagami, Risako Yasuda, Yukinori Ikegami, Takumi Toya, Yuji Nagatomo, Bonpei Takase, Takeshi Adachi

{"title":"Effectiveness of the serum ratio of l-cysteine to glutathione for the prediction of cardiovascular events.","authors":"Yuki Ishinoda, Nobuyuki Masaki, Yasuhiro Hitomi, Ryota Nakazawa, Akira Taruoka, Akane Kawai, Midori Iwashita, Yasuo Ido, Yusuke Yumita, Kazuki Kagami, Risako Yasuda, Yukinori Ikegami, Takumi Toya, Yuji Nagatomo, Bonpei Takase, Takeshi Adachi","doi":"10.3164/jcbn.24-156","DOIUrl":"10.3164/jcbn.24-156","url":null,"abstract":"Aminothiols play an important role in the antioxidant defense system. Their serum profile may be a predictor of prognosis and cardiovascular events. This study followed-up 262 patients (202 men; age, 65 ± 13 years) who had been treated for cardiovascular disease. The patients were divided into two groups by the median total l-cysteine to total glutathione (tCySH/tGSH) ratio in serum at enrollment. There were 32 (11%) all-cause deaths, 20 (8%) cardiovascular deaths, and 32 (12%) major cardiovascular events in 5.5 ± 3.2 years. Twenty-nine (11%) patients were hospitalized for heart failure. The high tCySH/tGSH ratio group (≥80.70) had a higher incidence of all-cause death than the low tCySH/tGSH ratio group (<80.70; log-rank test, p = 0.025). Multivariate Cox regression analysis adjusted for age, sex, body mass index, current smoking, renal function, and log10-transformed brain natriuretic peptide showed that the tCySH/tGSH ratio had predictive value for all-cause death, cardiovascular death, and heart failure. The adjusted hazard ratio for heart failure for the high versus low tCySH/tGSH ratio groups was 3.071 (95% confidence interval: 1.186-7.952; p = 0.021). The tCySH/tGSH ratio may be an useful biomarker to assess prognosis, cardiovascular events, and heart failure.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"77 2","pages":"174-181"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12440669/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Capturing systemic inflammation in a nonhuman primate using a new leukocyte activity assessment system. 使用新的白细胞活性评估系统捕获非人灵长类动物的全身炎症。

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-09-01 Epub Date: 2025-06-11 DOI: 10.3164/jcbn.25-65

Kozo Takeuchi, Shigeyuki Yamamoto, Yukiko Hatano, Dai Fukumoto, Takeharu Kakiuchi, Yoshiyuki Shimizu, Kimiko Kazumura

{"title":"Capturing systemic inflammation in a nonhuman primate using a new leukocyte activity assessment system.","authors":"Kozo Takeuchi, Shigeyuki Yamamoto, Yukiko Hatano, Dai Fukumoto, Takeharu Kakiuchi, Yoshiyuki Shimizu, Kimiko Kazumura","doi":"10.3164/jcbn.25-65","DOIUrl":"10.3164/jcbn.25-65","url":null,"abstract":"We developed an optical system that simultaneously monitors leukocytes producing the reactive oxygen species (ROS) superoxide and hypochlorite ions using only a few microliters of whole blood. Here, we report the first use of this system to measure circulating blood from a nonhuman primate. This observational pilot study involved rhesus monkeys recruited for a different purpose, brain positron emission tomography in the conscious state. The results indicated that (1) surgical inflammation influences the total ROS produced by leukocytes per unit of time; (2) blood analysis of a septic rhesus monkey exhibited a characteristic pattern of leukocyte ROS production; and (3) a strong positive correlation was observed between leukocyte ROS production and neutrophil counts at normal C-reactive protein (CRP) levels, which decreased under abnormal CRP levels. These findings suggest that the system can capture systemic inflammation in non-human primates and highlight the importance of monitoring leukocyte ROS production to understand inflammatory status. The difficulty of identifying subjective symptoms in veterinary medicine highlights the importance of technologies for easily and objectively monitor animals' physical conditions. Further analyses, including studies with larger animal populations and comparisons with conventional biomarkers, are needed to determine the specific inflammatory status reflected in leukocyte ROS production.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"77 2","pages":"162-173"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12440673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Poricoic acid a inhibits mitochondrial dysfunction in myocardial infarction by activating SIRT3. 茯苓酸a通过激活SIRT3抑制心肌梗死线粒体功能障碍。

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-09-01 Epub Date: 2025-04-19 DOI: 10.3164/jcbn.24-208

Jinzhu Yin, Qu Jin, Zhaozheng Liu

{"title":"Poricoic acid a inhibits mitochondrial dysfunction in myocardial infarction by activating SIRT3.","authors":"Jinzhu Yin, Qu Jin, Zhaozheng Liu","doi":"10.3164/jcbn.24-208","DOIUrl":"10.3164/jcbn.24-208","url":null,"abstract":"Acute myocardial infarction (MI) is the most severe clinical manifestation of ischemic heart disease. Despite this, the mechanisms that disrupt mitochondrial homeostasis and contribute to cardiomyocyte loss during MI are poorly understood, emphasizing the urgent need for new therapeutic interventions. Poricoic acid A (PAA), the principal active component of pachymaria, possesses a range of pharmacological effects. However, the specific role and mechanisms by which PAA addresses mitochondrial dysfunction in MI remain unclear. This study aims to elucidate the impact of PAA on MI and uncover its potential regulatory mechanisms. We developed MI cell models using mouse primary cardiomyocytes incubated in a Forma Steri-Cult chamber containing 1% oxygen, 94% nitrogen, and 5% carbon dioxide. Our results demonstrate that PAA significantly improves cardiomyocyte injury in hypoxia-induced mouse primary cardiomyocytes. Furthermore, PAA activates the AMP-activated protein kinase/peroxisome proliferator-activated receptor gamma coactivator 1-alpha/Sirtuin 3 (AMPK/PGC-1α/SIRT3) signaling pathway in hypoxia-induced mouse primary cardiomyocytes. PAA enhances the oxidative stress response in hypoxia-induced mouse primary cardiomyocytes by activating SIRT3. Additionally, it improves mitochondrial dysfunction in these cardiomyocytes and reduces apoptosis by activating SIRT3. In summary, PAA inhibits mitochondrial dysfunction associated with MI by activating SIRT3, indicating its promise as a therapeutic agent for MI.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"77 2","pages":"136-143"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12440666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationship between serum pyridoxal 5'-phosphate concentration and cognitive function in older Japanese. 老年日本人血清吡哆醛5′-磷酸浓度与认知功能的关系。

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-07-01 Epub Date: 2025-02-20 DOI: 10.3164/jcbn.24-233

Kotaro Itoh, Akiko Kuwabara, Rei Otsuka, Taiki Sugimoto, Takashi Sakurai, Shumpei Niida, Misora Ao, Kiyoshi Tanaka, Shigeo Takenaka, Hiroaki Kanouchi

{"title":"Relationship between serum pyridoxal 5'-phosphate concentration and cognitive function in older Japanese.","authors":"Kotaro Itoh, Akiko Kuwabara, Rei Otsuka, Taiki Sugimoto, Takashi Sakurai, Shumpei Niida, Misora Ao, Kiyoshi Tanaka, Shigeo Takenaka, Hiroaki Kanouchi","doi":"10.3164/jcbn.24-233","DOIUrl":"10.3164/jcbn.24-233","url":null,"abstract":"Deficiency of pyridoxal 5'-phosphate (PLP) causes neurological abnormalities. The decline in blood PLP concentration has been associated with the onset of dementia, but no studies have been conducted on Japanese. This study aimed to determine the relationship between serum PLP concentration and Alzheimer's disease (AD) in older Japanese individuals. A total of 266 participants, 84 healthy participants, 87 participants with mild cognitive impairment (MCI), and 95 patients with AD were randomly selected from those who visited the National Center for Geriatrics and Gerontology for Longevity Sciences. Serum PLP concentration was significantly lower in the AD compared to the NC. Participants were divided into quartiles (Q1-Q4). The relationship between quartiles of serum PLP concentration and cognitive function was analyzed using logistic regression analyses adjusted with covariate factors (sex, age, number of applicable frailty evaluations, APOE4, educational level, albumin, homocysteine, vitamin B1, B12, and folate). The odds ratios (ORs) for MCI or AD were significantly lower for Q3 and Q4 compared to Q1, and the ORs (95% CI) for Q3 and Q4 were 0.40 (0.16-0.98) and 0.37 (0.13-0.99), respectively. Lower serum PLP concentration is independently related to the incidence of MCI or AD. Further research is needed to clarify the causal relationship.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"77 1","pages":"79-84"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12326249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TFAP2C activates PTGES through the NOTCH3 signaling pathway to affect gefitinib resistance in lung adenocarcinoma. TFAP2C通过NOTCH3信号通路激活PTGES，影响肺腺癌患者吉非替尼耐药。

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-07-01 Epub Date: 2025-04-25 DOI: 10.3164/jcbn.24-104

Junmeng Xiao, Jianwei Cao, Lei Zhu, Jianbin Hou

{"title":"TFAP2C activates PTGES through the NOTCH3 signaling pathway to affect gefitinib resistance in lung adenocarcinoma.","authors":"Junmeng Xiao, Jianwei Cao, Lei Zhu, Jianbin Hou","doi":"10.3164/jcbn.24-104","DOIUrl":"10.3164/jcbn.24-104","url":null,"abstract":"Lung adenocarcinoma (LUAD) is one of the primary culprits of cancer-related deaths. Current treatment modalities for LUAD have certain limitations, necessitating innovating effective LUAD treatment strategies. Prostaglandin E synthase (PTGES) and TF activating protein 2C (TFAP2C) in the process of drug resistance in LUAD are less studied and need further in-depth research. This study aimed to investigate the specific molecular mechanisms of PTGES and TFAP2C in gefitinib resistance in LUAD. The results indicated that PTGES and TFAP2C were considerably overexpressed in LUAD tissues and cells. Chromatin immunoprecipitation and dual luciferase assay validated that TFAP2C targeted the PTGES promoter region. In addition, gene set enrichment analysis results demonstrated the notable enrichment of PTGES in the NOTCH3 signaling pathway. Overexpression of PTGES remarkably enhanced the viability of PC-9/GR (gefitinib-resistant) cells and their response to gefitinib resistance, which was reversed by the addition of a NOTCH3 inhibitor. Furthermore, overexpressing PTGES upon the TFAP2C silence restored the great inhibition effect conferred by TFAP2C silence in PC-9/GR cells on cell viability and cell response to gefitinib resistance. This study confirmed that TFAP2C can transcriptionally activate PTGES through the NOTCH3 signaling pathway to enhance the response of LUAD cells to gefitinib resistance, proffering a new approach for the treatment of gefitinib resistance in LUAD cells.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"77 1","pages":"1-9"},"PeriodicalIF":1.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12326243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144799260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kurarinone activates the Nrf-2/HO-1 signaling pathway and alleviates high glucose-induced ferroptosis in HK2 cells. Kurarinone激活Nrf-2/HO-1信号通路，缓解高糖诱导的HK2细胞铁下垂。

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-07-01 Epub Date: 2025-04-16 DOI: 10.3164/jcbn.24-210

Chunmei Ma

引用次数: 0