Journal of Clinical Biochemistry and Nutrition最新文献

{"title":"β-Elemene inhibits adipogenesis in 3T3-L1 cells by regulating AMPK pathway.","authors":"Xiang Deng, Zhenmin Liu, Sen Yang","doi":"10.3164/jcbn.24-179","DOIUrl":"10.3164/jcbn.24-179","url":null,"abstract":"<p><p>The prevalence of childhood obesity in global is quickly augmented, resulting into grievous public health problems and influencing adolescent development. β-Elemene is a sesquiterpene, and can extracted from traditional Chinese medicine-<i>Curcuma longa</i> L. β-Elemene has been discovered to display regulatory functions in multiple diseases, but it's roles in obesity need further investigations. The purpose of this work is to investigate the regulatory impacts of β-elemene on obesity progression and associated pathways. In this study, it was revealed that the heightened lipid accumulation in 3T3-L1 cells triggered by 3-isobutyl-1-methylxanthine + dexamethazone + insulin (MDI) can be restrained by β-elemene. Furthermore, β-elemene can modulate lipid metabolism in 3T3-L1 cells mediated by MDI. The glucose consumption was descended after insulin resistance treatment, but this impact was reversed after β-elemene treatment. At last, it was illustrated that the AMPK pathway was retarded after β-elemene induction, but this change was offset after β-elemene treatment. To sum up, our results manifested that β-elemene inhibited adipogenesis in 3T3-L1 cells, and evoked the AMPK pathway. This project may supply serviceable insights of β-elemene in the progression of obesity.</p>","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 2","pages":"125-130"},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the gut phageome of Japanese patients with ulcerative colitis under endoscopic remission.","authors":"Akinori Otsuki, Ryo Inoue, Takayuki Imai, Hiroto Miura, Atsushi Nishida, Osamu Inatomi, Akira Andoh","doi":"10.3164/jcbn.24-173","DOIUrl":"10.3164/jcbn.24-173","url":null,"abstract":"<p><p>This study aimed to analyze the gut phageome in Japanese patients with ulcerative colitis (UC) in endoscopic remission. Fecal samples were collected from 35 UC patients and 22 healthy controls. The gut microbiome was analyzed using 16S rRNA amplicon sequencing, and the phageome was profiled through shotgun metagenomic sequencing. Compared to healthy controls, UC patients showed a significant reduction in phageome richness (observed species and Chao1 index). Principal coordinate analysis revealed a significant difference in beta-diversity between UC and healthy controls (<i>p</i> = 0.001). The abundance of temperate phages was higher in UC (15.2%) compared to healthy controls (5.9%), although this was not statistically significant (<i>p</i> = 0.088). Temperate phages associated with <i>Coprococcus</i> sp., <i>Bacteroides</i> sp. KFT8, and <i>Faecalibacterium prausnitzii</i>, as well as virulent phages associated with <i>Ruminococcus gnavus</i> and <i>Lactobacillus farciminis</i>, were increased in UC patients. Conversely, phages associated with <i>Thermosipho affectus</i>, <i>Bacteroides</i> sp. OF03-11BH, and <i>Odoribacter splanchnicus</i> were decreased in UC patients. Phages associated with the genera <i>Odoribacter</i> (<i>p</i> = 0.0004), <i>Ruminococcus</i> (<i>p</i> = 0.009), and <i>Veillonella</i> (<i>p</i> = 0.013) were significantly reduced in UC patients. The gut phageome of inactive UC patients exhibited notable alterations in viral composition compared to healthy controls. These results suggest that changes in the gut phageome might be involved in the pathogenesis of UC.</p>","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 2","pages":"202-209"},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one-containing lactic acid bacterial beverages on skin moisture: a randomized, double-blind, placebo-controlled, parallel study.","authors":"Taiki Sato, Masato Tomizawa, Shuichi Segawa, Masao Matsuoka, Takashi Aurues","doi":"10.3164/jcbn.24-178","DOIUrl":"10.3164/jcbn.24-178","url":null,"abstract":"<p><p>Although we previously reported that 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one has antioxidant properties, its effect on the skin remains unclear. This study aimed to investigate the effects of beverages containing 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one on skin moisture. This study enrolled 220 healthy Japanese participants with dry skin who were randomly assigned to the test or placebo group (<i>n</i> = 110 each). Each group received either a beverage containing 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one or a placebo for 12 weeks. The primary outcome was stratum corneum water content. Secondary outcomes were transdermal water loss, number of blemishes and wrinkles, and blood antioxidant markers such as biological antioxidant potential and diacron-reactive oxygen metabolites. Visual analog scale was used to assess skin improvement. Stratum corneum water content and visual analog scale scores differed significantly between the test and placebo groups. Water content significantly increased in the test group compared to the placebo group at 4 and 8 weeks. Subjective skin symptoms significantly improved with the test beverage intake compared with the placebo. No other significant or adverse effects were observed. In conclusion, the of 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one-containing beverage for 12 consecutive weeks significantly increases stratum corneum water content. The study findings could aid in the development of safe functional foods enriched with this compound.</p>","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 2","pages":"195-201"},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut dysbiosis induced by a high-salt diet aggravates atherosclerosis by increasing the absorption of saturated fatty acids in ApoE-deficient mice.","authors":"Takashi Yoshimura, Takuro Okamura, Hiroki Yuge, Yukako Hosomi, Tomonori Kimura, Emi Ushigome, Naoko Nakanishi, Ryoichi Sasano, Takehiro Ogata, Masahide Hamaguchi, Michiaki Fukui","doi":"10.3164/jcbn.24-163","DOIUrl":"10.3164/jcbn.24-163","url":null,"abstract":"<p><p>Excessive salt intake has been associated with gut dysbiosis and increased cardiovascular risk. This study investigates the role of gut dysbiosis induced by a high-salt diet in the progression of atherosclerosis in ApoE-deficient mice. Sixteen-week-old male ApoE-deficient mice were fed either a high-fat, high-sucrose diet or high-fat, high-sucrose diet supplemented with 4% NaCl for eight weeks. The group on the HFHSD with high salt showed significant progression of atherosclerosis compared to the high-fat, high-sucrose diet group. Analysis of the gut microbiota revealed reduced abundance of beneficial bacteria such as <i>Allobaculum spp.</i>, <i>Lachnospiraceae</i>, and <i>Alphaproteobacteria</i> in the high-salt group. Additionally, this group exhibited increased expression of the Cd36 gene, a transporter of long-chain fatty acids, in the small intestine. Serum and aortic levels of saturated fatty acids, known contributors to atherosclerosis, were markedly elevated in the high-salt group. These findings suggest that a high-salt diet exacerbates atherosclerosis by altering gut microbiota and increasing the absorption of saturated fatty acids through upregulation of intestinal fatty acid transporters. This study provides new insights into how dietary salt can influence cardiovascular health through its effects on the gut microbiome and lipid metabolism.</p>","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 2","pages":"210-220"},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936735/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic mediators of the causal relationship between inflammatory bowel disease and allergic rhinitis: insights from Mendelian randomization.","authors":"Junyan Zhang, Huancheng Xie, Yuyi Huang","doi":"10.3164/jcbn.24-161","DOIUrl":"10.3164/jcbn.24-161","url":null,"abstract":"<p><p>Inflammatory bowel disease, including Crohn's disease and ulcerative colitis, is associated with various comorbidities, such as allergic diseases like allergic rhinitis. However, the causal relationship and potential metabolic mechanisms remain unclear. This study investigates the association between inflammatory bowel disease and allergic rhinitis, focusing on potential metabolic mediation through Mendelian randomization analysis. A two-sample Mendelian randomization analysis was conducted using datasets from European populations to evaluate the relationships between inflammatory bowel disease, Crohn's disease, ulcerative colitis, and allergic rhinitis. Additionally, 212 potential mediating metabolites were analyzed to explore metabolic mechanisms. Horizontal pleiotropy was excluded, and mediation analysis identified specific metabolites mediating these effects. Results revealed a significant association between inflammatory bowel disease, Crohn's disease, and allergic rhinitis, while ulcerative colitis showed no significant impact. Further analysis confirmed a unidirectional causal relationship from inflammatory bowel disease and Crohn's disease to allergic rhinitis. Metabolite analysis identified 91 significant metabolites, with 67 showing consistent effects. Notably, erythritol, 1-myristoyl-2-arachidonoyl-GPC, and the 3-methyl-2-oxovalerate to 4-methyl-2-oxopentanoate ratio exhibited significant mediation effects. These findings highlight a significant causal link between inflammatory bowel disease, particularly Crohn's disease, and allergic rhinitis, mediated by specific metabolites, offering new insights and potential targets for clinical interventions.</p>","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 2","pages":"187-194"},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploratory analysis of the association between dietary niacin intakes and nonalcoholic fatty liver disease among US adults: 1999-2018 data analysis from the National Health and Nutrition Examination Survey (NHANES).","authors":"Yue Chen, Xianwei Guo, Lei Hu, Wenzhi Yang, Ran Lin, Guodong Cao, Maoming Xiong, Bo Chen","doi":"10.3164/jcbn.23-63","DOIUrl":"10.3164/jcbn.23-63","url":null,"abstract":"<p><strong>Background: </strong>Previous researches have revealed the potential association between dietary niacin intakes and several diseases, but studies assessing the association between dietary niacin intakes and nonalcoholic fatty liver disease (NAFLD) is limited and remains unclear. This study was performed to explore the association.</p><p><strong>Methods: </strong>In this study, 10,528 participants (male: 5,257) in the 10 National Health and Nutrition Examination Survey (NHANES) cycles (1999-2018) from the NHANES database were selected for the analyses. We built three logistic regression models to explore the independent association between dietary niacin intakes and NAFLD and to explore whether such association exists. Finally, a restricted cubic spline model was applied to simulate the potential nonlinear association between dietary niacin intakes and the occurrence of NAFLD.</p><p><strong>Results: </strong>The result of the fully-adjusted model suggested that ln-transformed dietary niacin intakes were significantly associated with the reduced occurrence of NAFLD. The odd ratio (OR) of the model and its 95% confidence interval (CI) were 0.81 (0.73, 0.90). When taking the lowest quartile as a reference, the level of niacin in the highest quartile was associated with decreased prevalence of NAFLD (OR: 0.76, 95% CI: 0.63, 0.91). The restricted cubic spline plot presented a negative dose-response association between levels of daily niacin consumption and the occurrence of NAFLD (<i>p</i> for nonlinearity = 0.762).</p><p><strong>Conclusion: </strong>According to the results of this study, dietary niacin intakes may have a negative association with NAFLD, and more well-designed cohort studies are required in the future to confirm the obtained finding.</p>","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 2","pages":"179-186"},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936733/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the protective role of maternal lung cancer history on allergic rhinitis.","authors":"Junyan Zhang, Songsheng Wang, Yu-Yi Huang","doi":"10.3164/jcbn.24-172","DOIUrl":"10.3164/jcbn.24-172","url":null,"abstract":"<p><strong>Background: </strong>The causal relationship between family history of lung cancer and allergic rhinitis remains unclear. This study aimed to explore the association between family history of lung cancer and allergic rhinitis, along with potential mediating mechanisms, using Mendelian randomization.</p><p><strong>Methods: </strong>A bidirectional two-sample Mendelian randomization analysis was conducted to assess the causal relationship between family history of lung cancer (including parental, paternal, maternal, and sibling histories) and allergic rhinitis, using genetic variants associated with family history of lung cancer as instrumental variables. Additionally, mediation Mendelian randomization analysis was performed to investigate the role of specific metabolites in mediating this relationship.</p><p><strong>Results: </strong>The analysis revealed a significant causal relationship between parental history of lung cancer and allergic rhinitis, with maternal lung cancer history showing a strong protective effect against allergic rhinitis (OR = 0.28, <i>p</i><0.05). Mediation analysis further indicated that metabolites such as 1-linoleoyl-GPE (18:2) and <i>N</i>-palmitoyl-sphingosine exhibited negative mediating effects in the association between maternal lung cancer and allergic rhinitis. Lower levels of these metabolites enhanced the protective effect of maternal lung cancer history on allergic rhinitis.</p><p><strong>Conclusion: </strong>This study demonstrates a significant causal relationship between maternal lung cancer history and allergic rhinitis, with specific metabolites potentially playing a mediating role. Changes in the levels of 1-linoleoyl-GPE (18:2) and <i>N</i>-palmitoyl-sphingosine are associated with the protective effect of maternal lung cancer history on allergic rhinitis, suggesting that metabolites may be crucial in regulating this relationship. These findings provide new insights into the relationship between family history of lung cancer and immune-related diseases, offering potential directions for future clinical prevention and treatment strategies.</p>","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 2","pages":"156-163"},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing vitamin supplementation via reference interval update of vitamins A, E, B1, and B6 using HPLC.","authors":"Andrea Caballero, Gonzalo Gonzalez-Silva, Pablo Gabriel-Medina, Marc Cuadros, Alfonso Ayora, Albert Blanco-Grau, Víctor Martin-Riera, Laura Conesa, Fernando Moreno, Sarai Garriga-Edo, Lydia Peris-Serra, Clara Sanz-Gea, Yolanda Villena","doi":"10.3164/jcbn.24-155","DOIUrl":"10.3164/jcbn.24-155","url":null,"abstract":"<p><p>Vitamins are essential micronutrients obtained from the diet, required by the body in small amounts daily for proper metabolism. Monitoring their levels is necessary for detecting deficiencies and guiding supplementation in certain clinical conditions. This study aimed to update the reference values for vitamins A, B1, B6, and E, and some related ratios, adjusted to the adult population of our health reference area using liquid chromatography in a direct approach calculation (<i>n</i> = 146, age: 21-64 years, 64% females). No significant differences in vitamin levels or ratios were observed based on age and sex. We obtained reliable and updated reference values: 1.1-2.8 ‍μmol/L and 18.9-42.2 ‍μmol/L for vitamins A and E respectively, 85.9-181.6 nmol/L and 57.0-165.7 nmol/L for vitamins B1 and B6 respectively; and related ratios of 246.2-561.1 ‍ng/g for vitamin B1 corrected by hemoglobin; 5.2-8.9 ‍μmol/mmol and 4.5-7.4 ‍μmol/mmol for vitamin E corrected by cholesterol and total lipids, respectively. These reference values significantly differ from those provided by the reagent manufacturer currently in use. While correcting vitamin E for lipids and vitamin B1 for hemoglobin is not recommended for the general population, these adjustments may be useful in interpreting results in certain pathological conditions.</p>","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 2","pages":"148-155"},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The protective role of kakkalide in sepsis-induced intestinal barrier dysfunction via inhibition of NF-κB pathway activation.","authors":"Tongrong Xu, Jiahui Han, Nan Wang, Zhirong Huan, Hao Yao, Xin Ge","doi":"10.3164/jcbn.24-182","DOIUrl":"10.3164/jcbn.24-182","url":null,"abstract":"<p><p>Sepsis, a systemic inflammatory response often triggered by infection, can lead to multi-organ failure, with the intestine being one of the most vulnerable organs. The nuclear factor kappa-B (NF-κB) pathway plays a crucial role in immune responses, inflammation, and cell survival, making it central to sepsis-induced intestinal damage. Kakkalide (KA), a bioactive compound known for its anti-inflammatory, cardiovascular, neuroprotective, and anti-diabetic properties, has potential therapeutic effects. However, its impact on sepsis-induced intestinal injury remains unclear. In this study, murine sepsis models were used both <i>in vivo</i> and <i>in vitro</i> to evaluate the protective effects of KA on intestinal histopathology, apoptosis, and inflammation. Results showed that KA significantly reduced intestinal damage and apoptosis, as evidenced by hematoxylin-eosin and TUNEL staining. KA also improved intestinal barrier integrity, as indicated by reduced diamine oxidase activity, d-lactic acid content, and fluorescein isothiocyanate intensity, along with increased expression of zonula occludens-1. Furthermore, KA alleviates inflammation by reducing the levels of tumor necrosis factor-α, interleukin-1β, prostaglandin E2, inducible nitric oxide synthase, and cyclooxygenase-2. Immunofluorescence and Western blot analysis revealed that KA inhibited the sepsis-induced phosphorylation of inhibitor-kappaBα and RelA (P65) and prevented P65's translocation to the nucleus. These findings were confirmed in lipopolysaccharide-induced Caco-2 cells, suggesting that KA protected the intestinal barrier during sepsis by suppressing the NF-κB pathway.</p>","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 2","pages":"139-147"},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936737/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary coconut oil lowered circulating fetuin-A levels and hepatic expression of fetuin-A in KK/TaJcl mice.","authors":"Yuzuru Iizuka, Chikako Kitagawa, Towa Tamura, Hidehiro Ueshiba, Satoshi Hirako, Toshifumi Osaka, Hyounju Kim, Naoko Yanagisawa","doi":"10.3164/jcbn.24-160","DOIUrl":"10.3164/jcbn.24-160","url":null,"abstract":"<p><p>Although coconut oil has attracted great attention as a functional food, enough supportive scientific evidence is lacking. In addition, the beneficial effects of coconut oil consumption on the prevention of metabolic disorders are controversial. Fetuin-A is a plasma glycoprotein secreted by hepatocytes and adipocytes. Circulating fetuin-A levels relate to insulin resistance due to macrophage-mediated adipose tissue inflammation. This study demonstrated that coconut oil feeding significantly downregulated the hepatic expression of fetuin-A and reduced its plasma level in KK mice-an obese diabetic model animal. The expression of monocyte chemoattractant protein-1, a potent inducer for macrophage infiltration, decreased in epididymal white adipose tissue in coconut oil-fed KK mice. The expression of CD68 and CD11c, markers of proinflammatory M1 macrophages, was significantly reduced by coconut oil feeding in epididymal white adipose tissue of KK mice. However, the mice did not exhibit improved insulin resistance. Our results may further support the potential of coconut oil as a dietary trigger that can reduce both circulating fetuin-A levels and infiltration of proinflammatory macrophages in visceral adipose tissue.</p>","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 2","pages":"131-138"},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}