Journal of Clinical Biochemistry and Nutrition最新文献_第2页

The effects of lutein, zeaxanthin and anthocyanins supplementation on visual function and vision protection in healthy school-aged children: a single-center prospective randomized controlled study. 补充叶黄素、玉米黄质和花青素对健康学龄儿童视觉功能和视力保护的影响：一项单中心前瞻性随机对照研究

IF 1.9 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2026-07-01 Epub Date: 2026-03-19 DOI: 10.3164/jcbn.25-284

Zixiu Zhou, Pengjie Zheng, Sizhen Li, Qingsong Yang, Shanshan Xu, Na Zhao, Jinshuo Wang, Yuan Shi, Qidong Lu, Zhengyuan Zhou, Annan Xu

{"title":"The effects of lutein, zeaxanthin and anthocyanins supplementation on visual function and vision protection in healthy school-aged children: a single-center prospective randomized controlled study.","authors":"Zixiu Zhou, Pengjie Zheng, Sizhen Li, Qingsong Yang, Shanshan Xu, Na Zhao, Jinshuo Wang, Yuan Shi, Qidong Lu, Zhengyuan Zhou, Annan Xu","doi":"10.3164/jcbn.25-284","DOIUrl":"10.3164/jcbn.25-284","url":null,"abstract":"Dietary carotenoids (lutein and zeaxanthin) and anthocyanins show retinal protective potential, yet their clinical efficacy in healthy children's developing visual systems remains unclear. We aimed to investigate whether lutein, zeaxanthin, and anthocyanin (LA) supplementation enhances visual function and vision protection in healthy school-aged children (6-10 years). Sixty-four participants were randomized to control or treatment (received LA supplementation daily) groups. Macular pigment optical density (MPOD), central foveal thickness (CFT), minimum foveal thickness (MFT), axial length (AL), spherical equivalent refraction (SER) and contrast sensitivity function (CSF) after LA supplementation were examined at baseline, 1, 3, and 6 months. ASQ-11 questionnaire was used to evaluate eye fatigue. Compared with control group, from baseline to 1, 3, and 6 months, MPOD levels were increased in treatment group, with the most significant increase at the 6-month follow-up. CFT and MFT levels were also elevated with time. Moreover, AL remarkably increased at 1, 3, and 6 months, considering it is a physiological increase caused by growth and development of children. At 6 months, the change in AL was prominently reduced in the treatment group. Besides, the reduction of SER levels was less in treatment group than that in control group. Additionally, CSF of control group and treatment group increased during the 6-month follow-up. CSF at spatial frequency 3 c/d in the treatment group was markedly higher than that in the control group at the sixth month follow-up. Furthermore, LA notably improved visual fatigue symptoms. Collectively, LA supplementation enhances visual function and vision protection in healthy school-aged children. Registration number (TRN): ChiCTR2500100376.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"79 1","pages":"52-59"},"PeriodicalIF":1.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13412534/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148620421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanistic insights and evidence-based management of metabolic dysfunction-associated steatotic liver disease (MASLD; formerly NAFLD) through combined diet and exercise interventions: an evidence-based narrative review. 通过饮食和运动联合干预代谢功能障碍相关脂肪性肝病（MASLD）的机制见解和循证管理：一项基于证据的叙事回顾。

IF 1.9 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2026-07-01 Epub Date: 2026-03-19 DOI: 10.3164/jcbn.25-259

Yuanyuan Chen, Rongqin Liu, Renli Huang, Qinwei Li, Yu Zhang

{"title":"Mechanistic insights and evidence-based management of metabolic dysfunction-associated steatotic liver disease (MASLD; formerly NAFLD) through combined diet and exercise interventions: an evidence-based narrative review.","authors":"Yuanyuan Chen, Rongqin Liu, Renli Huang, Qinwei Li, Yu Zhang","doi":"10.3164/jcbn.25-259","DOIUrl":"10.3164/jcbn.25-259","url":null,"abstract":"Metabolic dysfunction-associated steatotic liver disease (MASLD; formerly NAFLD) is increasing worldwide, while management is constrained by limited effective pharmacotherapies and poor long-term adherence to lifestyle change. We synthesized mechanistic pathways and primary human interventional evidence on combined diet-plus-exercise strategies to inform lifestyle management. Evidence-based narrative review with a structured literature search and a predefined evidence framework. We prioritized primary human interventional studies (randomized or controlled trials when available) evaluating combined dietary and exercise interventions in MASLD/NAFLD; mechanistic studies were used for biological context. Reviews/meta-analyses were used only to summarize existing evidence and were not treated as additional primary studies. Owing to heterogeneity in interventions and outcomes, we performed qualitative synthesis (no de novo meta-analysis). Evidence indicates that combined dietary and exercise interventions exert synergistic effects on key metabolic and inflammatory pathways, including AMPK/SIRT1 activation, mitochondrial biogenesis, and gut-liver axis modulation, thereby improving hepatic steatosis and metabolic profiles more effectively than single interventions. Integrated dietary and exercise interventions appear more effective than diet- or exercise-only strategies for MASLD/NAFLD management. Individualized programs supported by digital health technologies and multi-omics profiling may further optimize lifestyle intervention delivery and move care toward a precision-oriented model.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"79 1","pages":"1-14"},"PeriodicalIF":1.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13412594/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148620367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of body position on high-resolution manometry parameters and their correlations with symptoms. 体位对高分辨率测压参数的影响及其与症状的相关性。

IF 1.9 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2026-07-01 Epub Date: 2026-04-23 DOI: 10.3164/jcbn.25-282

Sayaka Yoshida, Hideki Mori, Tatsuhiro Masaoka, Juntaro Matsuzaki, Kazumi Inokuchi, Hiroko Ando, Takanori Kanai

{"title":"Effects of body position on high-resolution manometry parameters and their correlations with symptoms.","authors":"Sayaka Yoshida, Hideki Mori, Tatsuhiro Masaoka, Juntaro Matsuzaki, Kazumi Inokuchi, Hiroko Ando, Takanori Kanai","doi":"10.3164/jcbn.25-282","DOIUrl":"10.3164/jcbn.25-282","url":null,"abstract":"High-resolution manometry (HRM) is essential for evaluating esophageal motility, and the Chicago Classification ver. 4.0 recommends measurements in both supine and upright positions. However, the impact of body position on HRM metrics and their relationship with symptoms has not been fully clarified. In this retrospective observational study, 287 patients undergoing HRM were categorized into postoperative, disorders of esophagogastric junction outflow (DEO), peristaltic disorders, and normal motility groups. Integrated relaxation pressure (IRP), distal contractile integral (DCI), and intrabolus pressure (IBP) were assessed in both positions, and symptom severity was evaluated using the Eckardt score, Gastroesophageal Reflux Disease Questionnaire (GerdQ), and Hospital Anxiety and Depression Scale (HADS). IRP and DCI were significantly lower in the upright position, whereas IBP showed no positional difference. In the DEO group, IRP correlated with the Eckardt score in both positions, with a slightly stronger correlation in the supine position. Reflux and anxiety symptoms also correlated with the Eckardt score, suggesting interactions between motility impairment, reflux, and psychological factors. Although HRM metrics varied by position, neither position was clearly superior for explaining symptoms. HRM in both supine and upright positions remains essential for comprehensive clinical assessment.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"79 1","pages":"97-103"},"PeriodicalIF":1.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13412535/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148620427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Topical Mongolian Lycium ruthenicum extract attenuates oxidative stress, inflammation, and itch signaling in atopic dermatitis models. 外用蒙古枸杞提取物可减轻特应性皮炎模型中的氧化应激、炎症和瘙痒信号。

IF 1.9 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2026-07-01 Epub Date: 2026-05-15 DOI: 10.3164/jcbn.26-41

Himeno Saito, Chizuki Usui, Reo Matsuzaka, Chiharu Ohira, Mao Kaneki, Yoshiichi Takagi, Yoshikazu Hoshino, Tomoki Fukuyama

{"title":"Topical Mongolian Lycium ruthenicum extract attenuates oxidative stress, inflammation, and itch signaling in atopic dermatitis models.","authors":"Himeno Saito, Chizuki Usui, Reo Matsuzaka, Chiharu Ohira, Mao Kaneki, Yoshiichi Takagi, Yoshikazu Hoshino, Tomoki Fukuyama","doi":"10.3164/jcbn.26-41","DOIUrl":"10.3164/jcbn.26-41","url":null,"abstract":"Mongolian Lycium ruthenicum (LR) are rich in anthocyanins and oligomeric proanthocyanidins, which possess potent antioxidant and anti-inflammatory properties. However, their therapeutic potential in inflammatory skin diseases, such as atopic dermatitis (AD), remains unclear. This study investigated the antioxidant and anti-inflammatory properties of topical LR extract and its therapeutic potential in experimental models of AD. Antioxidant activity was assessed in UVB-irradiated human keratinocytes (HaCaT) by measuring intracellular reactive oxygen species (ROS) and the expression of Nrf2 and HO-1. Anti-inflammatory effects were examined in TNF-α/IFN-γ-stimulated HaCaT and LPS-induced DC2.4 cells. In vivo efficacy was evaluated in NC/nga and HR-1 mouse models of AD. LR extract significantly suppressed UVB-induced ROS production and enhanced Nrf2 and HO-1 expression. It reduced pro-inflammatory cytokine and chemokine levels by inhibiting ERK phosphorylation in HaCaT cells. Topical LR treatment in vivo attenuated trans-epidermal water loss, epidermal hyperplasia and immune cell infiltration, and Th2 cytokine levels in the lymph nodes. Dorsal root ganglion calcium imaging revealed decreased neuronal responsiveness to pruritogens following LR application. Topical application of LR extract ameliorated AD-like inflammation and pruritus through antioxidative and immunomodulatory mechanisms. These findings highlight LR as a natural therapeutic agent for managing oxidative stress-related skin inflammation and barrier dysfunction.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"79 1","pages":"111-124"},"PeriodicalIF":1.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13412595/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148620525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanism of enteral nutrition in regulating Tfh/Tfr immune imbalance in colorectal cancer via the TLR4 signaling pathway. 肠内营养通过TLR4信号通路调节结直肠癌中Tfh/Tfr免疫失衡的机制

IF 1.9 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2026-07-01 Epub Date: 2026-04-03 DOI: 10.3164/jcbn.25-260

Ming Zhang, LingYong Kong, TengFei Chen, Chuang Ding

{"title":"Mechanism of enteral nutrition in regulating Tfh/Tfr immune imbalance in colorectal cancer via the TLR4 signaling pathway.","authors":"Ming Zhang, LingYong Kong, TengFei Chen, Chuang Ding","doi":"10.3164/jcbn.25-260","DOIUrl":"10.3164/jcbn.25-260","url":null,"abstract":"This study aims to investigate the regulatory effects and underlying mechanisms of enteral nutrition (EN) on colorectal cancer (CRC). An inflammatory colorectal tumor model was established in mice using the azoxymethane-dextran sulfate sodium method. The mice were divided into groups including a model group and groups treated with EN or EN plus the Toll-like receptor 4 (TLR4) activator lipopolysaccharide. The T follicular helper/T follicular regulatory (Tfh/Tfr) cell ratios in splenic tissues were analyzed by flow cytometry. Protein expression related to the TLR4 pathway and cytokines in colonic tissues was assessed by Western blotting and quantitative real-time polymerase chain reaction. Histopathological changes were evaluated using hematoxylin-eosin staining. The proportion of Tfh cells to Tfr cells is significantly imbalanced in CRC, and this imbalance was corrected following EN treatment. This correction of Tfh/Tfr imbalance was found to inhibit tumor growth, suggesting that the regulatory effect of EN on CRC may be mediated through the modulation of the Tfh/Tfr cell balance. At the molecular level, we discovered that EN was found to suppress the activation of the TLR4 signaling pathway. Furthermore, the addition of lipopolysaccharide abolished the effects of EN, indicating that the regulation of the Tfh/Tfr cell ratio by EN is mediated through the TLR4 signaling pathway. This study provides evidence that EN can inhibit the progression of CRC, potentially by regulating Tfh/Tfr immune imbalance via the TLR4 signaling pathway. These findings highlight the potential of EN as a therapeutic strategy for CRC treatment.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"79 1","pages":"69-75"},"PeriodicalIF":1.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13412593/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148620400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine learning identifies neutrophil-related signatures for diagnostic value in neonatal sepsis. 机器学习识别中性粒细胞相关特征对新生儿败血症的诊断价值。

IF 1.9 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2026-07-01 Epub Date: 2026-03-19 DOI: 10.3164/jcbn.25-270

Qibing Chen, Jiandong Chen, Ronghua Zhong

{"title":"Machine learning identifies neutrophil-related signatures for diagnostic value in neonatal sepsis.","authors":"Qibing Chen, Jiandong Chen, Ronghua Zhong","doi":"10.3164/jcbn.25-270","DOIUrl":"10.3164/jcbn.25-270","url":null,"abstract":"Neonatal sepsis (NS) is one of the leading causes of neonatal mortality. The nonspecific clinical manifestations and the limited timeliness of existing biomarkers (such as C-reactive protein) highlight the urgent need for highly accurate diagnostic tools. Neutrophils, as key effector cells of innate immunity, are closely involved in the progression of NS. This study integrated training (GSE69686) and validation (GSE25504) datasets from the GEO database. Neutrophil infiltration characteristics were analyzed utilizing CIBERSORT, and weighted gene co-expression network analysis (WGCNA) was introduced to determine neutrophil-related co-expression modules. Three machine learning algorithms-LASSO, SVM-RFE, and RF-were implemented to cross-screen core diagnostic genes. A combined diagnostic model was distributed based on these genes. NetworkAnalyst was utilized to predict miRNA-TF regulatory networks, and GSVA was conducted to interpret biological functions. Three algorithms identified IL1R2 and METTL7B as core diagnostic genes; the model showed strong reliability. IL1R2 high expression correlated with reduced CD8+ T cells, regulatory T cells, and neutrophils (p<0.05). METTL7B high expression linked positively to B cells and negatively to NK cells/neutrophils. The two genes synergistically cause immune cell dysfunction. Six miRNAs and 15 transcription factors (e.g., NFKB1/RELA, STAT3) regulating these genes were found, involved in inflammation and metabolic reprogramming. Integrating neutrophil infiltration and triple-machine-learning, this study first proposed an IL1R2/METTL7B two-gene panel. The model had high accuracy and generalizability, potentially contributing to NS pathogenesis via immune dysfunction and metabolic reprogramming, supporting rapid diagnostics and targeted interventions.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"79 1","pages":"40-51"},"PeriodicalIF":1.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13412481/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148620406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trace elements in relation to glycemic control and disease complications in children living with type 1 diabetes mellitus. 微量元素与1型糖尿病儿童血糖控制和疾病并发症的关系

IF 1.9 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2026-07-01 Epub Date: 2026-04-28 DOI: 10.3164/jcbn.26-3

Randa Ibrahim, Amal Ahmed Mohamed, Enas Sayed Abbas, Eman Sultan, May Mohsen Samy Selim, Lamees Ahmed Samy, Eman M Fahmy, Mohamed AbdelFattah Ibrahim Saad, Omnia M Anwer, Mina Wassef Girgiss, Mohamed El Sherbiny, Yasser Batota, Rahmathullah Mohammad, Wael Hafez

{"title":"Trace elements in relation to glycemic control and disease complications in children living with type 1 diabetes mellitus.","authors":"Randa Ibrahim, Amal Ahmed Mohamed, Enas Sayed Abbas, Eman Sultan, May Mohsen Samy Selim, Lamees Ahmed Samy, Eman M Fahmy, Mohamed AbdelFattah Ibrahim Saad, Omnia M Anwer, Mina Wassef Girgiss, Mohamed El Sherbiny, Yasser Batota, Rahmathullah Mohammad, Wael Hafez","doi":"10.3164/jcbn.26-3","DOIUrl":"10.3164/jcbn.26-3","url":null,"abstract":"Trace elements such as copper, zinc, and magnesium are essential for antioxidant defense, insulin action and glucose homeostasis, yet their clinical utility as biomarkers in childhood type 1 diabetes mellitus remains uncertain. We conducted a case-control study of 199 Egyptian children aged 5-18 years (100 with type 1 diabetes mellitus and 99 non-diabetic controls) attending the National Nutrition Center. Anthropometry, lifestyle characteristics and biochemistry were assessed, and serum copper, zinc, and magnesium were measured by atomic absorption; the copper to zinc ratio was calculated. Children with type 1 diabetes mellitus had significantly lower zinc and magnesium concentrations and higher copper to zinc ratios than controls, while mean copper levels did not differ significantly. Lower magnesium and zinc, higher body mass index, elevated triglycerides, and reduced physical activity were independently associated with type 1 diabetes mellitus. In receiver operating characteristic analyses, magnesium showed the best discriminative performance between diabetic and control children, whereas zinc and the copper to zinc ratio were highly specific markers of poor glycemic control, and zinc deficiency and reduced estimated glomerular filtration rate were consistently associated with microvascular complications. These findings support trace-element monitoring to refine risk stratification and management in pediatric type 1 diabetes mellitus.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"79 1","pages":"86-96"},"PeriodicalIF":1.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13412591/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148620508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbonyl stress by glyceraldehyde promotes glycation of nuclear non-muscle myosins in DNA-protein crosslink assemblies. 甘油醛的羰基胁迫促进dna -蛋白质交联组装中核非肌球蛋白的糖基化。

IF 1.9 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2026-07-01 Epub Date: 2026-03-19 DOI: 10.3164/jcbn.26-18

Kenji Takeda, Hironobu Akao, Akiko Sakai-Sakasai, Kouji Kajinami, Masayoshi Takeuchi

{"title":"Carbonyl stress by glyceraldehyde promotes glycation of nuclear non-muscle myosins in DNA-protein crosslink assemblies.","authors":"Kenji Takeda, Hironobu Akao, Akiko Sakai-Sakasai, Kouji Kajinami, Masayoshi Takeuchi","doi":"10.3164/jcbn.26-18","DOIUrl":"10.3164/jcbn.26-18","url":null,"abstract":"DNA-protein crosslinks are bulky lesions that block DNA replication and transcription and are increasingly recognized in metabolic disorders characterized by carbonyl stress. Glyceraldehyde is a highly reactive triose that forms advanced glycation end-products, but its contribution to nuclear DNA-protein crosslink formation has not been defined. We examined glyceraldehyde-induced DNA-protein crosslinks in rat cardiomyoblast-derived H9c2 cells using an advanced recovery of potassium sodium dodecyl sulfate precipitates (ARK) assay combined with liquid chromatography-tandem mass spectrometry. Glyceraldehyde exposure modestly but significantly increased DNA-protein crosslink levels and generated nuclear high-molecular-weight complexes enriched in glycation adducts. Proteomic profiling of the DNA-tethered protein fraction revealed non-muscle myosin heavy chain isoforms 9 and 10 among the most abundant components. Liquid chromatography-tandem mass spectrometry mapping identified glycation adducts on multiple arginine residues within the coiled-coil rod and non-helical tail regions of non-muscle myosin heavy chains, including N δ-(5-hydro-5-methyl-4-imidazolon-2-yl)-ornithine and argpyrimidine. These modifications coincided with detergent-insoluble, high-molecular-weight myosin species in nuclear fractions. Our data identify glycated non-muscle myosins as major constituents of glyceraldehyde-induced, DNA-tethered DPC-associated assemblies and suggest a link between carbonyl stress and DNA damage associated with nuclear protein aggregation in cardiomyoblasts.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"79 1","pages":"28-39"},"PeriodicalIF":1.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13412479/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148620389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Homocysteine increases phosphorylation and aggregation of α-synuclein. 同型半胱氨酸增加α-突触核蛋白的磷酸化和聚集。

IF 1.9 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2026-05-01 Epub Date: 2026-03-11 DOI: 10.3164/jcbn.25-119

Soichi Enomoto, Tadanori Hamano, Rei Asano, Hirohito Sasaki, Tomohisa Yamaguchi, Yuki Kitazaki, Yoshinori Endo, Norimichi Shirafuji, Shu-Hui Yen, Yasuhisa Sawai, Hiroyuki Nakano, Hidenori Onishi, Masamichi Ikawa, Osamu Yamamura, Koji Aoki, Masashi Okuro, Yasunari Nakamoto, Yasuhiro Nishiyama

{"title":"Homocysteine increases phosphorylation and aggregation of α-synuclein.","authors":"Soichi Enomoto, Tadanori Hamano, Rei Asano, Hirohito Sasaki, Tomohisa Yamaguchi, Yuki Kitazaki, Yoshinori Endo, Norimichi Shirafuji, Shu-Hui Yen, Yasuhisa Sawai, Hiroyuki Nakano, Hidenori Onishi, Masamichi Ikawa, Osamu Yamamura, Koji Aoki, Masashi Okuro, Yasunari Nakamoto, Yasuhiro Nishiyama","doi":"10.3164/jcbn.25-119","DOIUrl":"10.3164/jcbn.25-119","url":null,"abstract":"The hyperphosphorylation of α-synuclein (α-Syn) at serine 129 is associated with in its aggregation, culminating in the formation of Lewy bodies (LBs) in Parkinson's disease (PD) and dementia with LBs. Plasma homocysteine (Hcy) levels are typically high in PD, particularly in those treated with Levodopa. Therefore, we aimed to investigate the effects of Hcy on phosphorylation and aggregation of α-Syn. The human neuroblastoma cell line 3D5 expressing wild-type α-Syn under the control of a tetracycline-off system was treated with Hcy, and aggregation and phosphorylation of α-Syn were examined. Hcy was cytotoxic to 3D5 cells, and Hcy induced cell shrinkage at a concentration >100 ‍μM. Treatment with Hcy upregulated the levels of α-Syn, increased its phosphorylation at serine 129, and increased casein kinase 2A. Moreover, Hcy treatment significantly increased the level of aggregated form of α-Syn, including oligomers in 3D5 cells. However, folate significantly reversed the Hcy-mediated increase in the levels of total α-Syn and its phosphorylation. In conclusion, Hcy enhances the total α-Syn level, and phosphorylated and oligomeric α-Syn formation, thereby promoting LBs formation.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"78 3","pages":"259-267"},"PeriodicalIF":1.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13193807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148015508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The ratio of TG/CysC is a significant indicator of nephritis in children with IgA vasculitis. TG/CysC比值是IgA血管炎患儿肾炎的重要指标。

IF 1.9 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2026-05-01 Epub Date: 2026-02-11 DOI: 10.3164/jcbn.25-242

Xiucui Han, Pengfei Xu

{"title":"The ratio of TG/CysC is a significant indicator of nephritis in children with IgA vasculitis.","authors":"Xiucui Han, Pengfei Xu","doi":"10.3164/jcbn.25-242","DOIUrl":"10.3164/jcbn.25-242","url":null,"abstract":"The incidence of IgA vasculitis (IgAV) in children is relatively high. Cases with severe kidney involvement can lead to death. This study aimed to assess whether the ratio of triglyceride and cystatin C (TG/CysC) represented a risk factor for nephritis in children with IgAV. A total of 451 children were recruited in this study, including 64 children with IgAV and 387 with IgAV with nephritis (IgAVN), according to renal puncture results. Triglycerides, creatinine, urea, uric acid, cystatin C, immunoglobulin A, complement C3, and complement C4 were measured. Compared with the IgAV group, the children with IgAVN had higher triglycerides, creatinine, urea, uric acid and TG/CysC and lower cystatin C and complement C4 (p<0.05). Stepwise multiple logistic regression models showed that TG/CysC (odds ratio: OR = 3.246) was an independent indicator of the presence of nephritis in children with IgAV as well as age (OR = 1.233), creatinine (OR = 1.052), and complement C4 (OR = 0.007). Furthermore, after adjustment for confounding variables, the prevalence of nephritis in children with IgAV was significantly higher according to increased serum TG/CysC levels. The adjusted ORs for nephritis according to TG/CysC tertiles were 1.00 (as references), 2.202 (1.117-4.342), and 14.188 (4.576-43.992). When the TG/CysC ratio was set as 1.94, the diagnostic sensitivity was 53%, and the specificity was 90.6%. Our data suggested that high levels of TG/CysC in children with IgAV was an increased risk for developing nephritis.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"78 3","pages":"214-218"},"PeriodicalIF":1.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13193792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148015626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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