Identification of miRNAs associated with eosinophilic esophagitis shown by esophageal mucosal biopsy results of Japanese patients.

IF 2 4区 医学 Q3 NUTRITION & DIETETICS
Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-05-01 Epub Date: 2024-12-28 DOI:10.3164/jcbn.24-193
Naoki Oshima, Norihisa Ishimura, Satoshi Kotani, Tomotaka Yazaki, Akihiko Oka, Nobuhiko Fukuba, Yoshiyuki Mishima, Hiroshi Tobita, Kotaro Shibagaki, Kousaku Kawashima, Yoshikazu Kinoshita, Shunji Ishihara
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引用次数: 0

Abstract

The number of eosinophilic esophagitis (EoE) patients has been rapidly increasing worldwide in recent years, though remains relatively lower in Japan. To determine whether Japanese EoE cases have pathogenic differences, microRNA (miRNA) transcriptome analyses of esophageal biopsy specimens from patients with EoE were performed to identify involved miRNAs and the results were compared with those for cases reported in Western countries. Japanese patients with EoE (n = 9) or reflux esophagitis (RE) (n = 4) were enrolled, with the latter serving as controls. Biopsies of esophageal mucosal tissue were performed as a part of an upper gastrointestinal endoscopy, followed by comprehensive miRNA expression analysis (2,588 target human miRNAs) using microarray technology. The EoE patients were then treated with a proton pomp inhibitor, and miRNA expression in the esophagus was compared before and after treatment. Hierarchical cluster analysis findings showed clear differences in miRNA expression patterns between the EoE and RE patients, with increased expression of 13 and decreased expression of 10 miRNAs noted in the EoE cases. These results were then compared with miRNA expression in esophageal mucosa of EoE patients presented in Western reports and the expression profiles were found to be very similar. In addition, changes in expression profiles of several miRNAs before and after treatment were observed in the present EoE patients. miRNA microarray analysis of Japanese EoE patients demonstrated a significant overlap of miRNA expression in comparison with Western patients, indicating that EoE likely represents the same disease among diverse racial populations and shows a consistent pathogenesis worldwide.

日本患者食管粘膜活检结果显示与嗜酸性粒细胞性食管炎相关的mirna鉴定。
近年来,嗜酸性粒细胞性食管炎(EoE)患者的数量在全球范围内迅速增加,尽管在日本仍然相对较低。为了确定日本EoE病例是否存在致病性差异,我们对EoE患者的食管活检标本进行了microRNA (miRNA)转录组分析,以确定涉及的miRNA,并将结果与西方国家报告的病例进行了比较。入选的日本EoE患者(n = 9)或反流性食管炎(RE)患者(n = 4),后者作为对照。作为上消化道内窥镜检查的一部分,对食管粘膜组织进行活检,然后使用微阵列技术进行全面的miRNA表达分析(2,588个目标人类miRNA)。然后对EoE患者进行质子泵抑制剂治疗,比较治疗前后食管miRNA的表达。分层聚类分析结果显示,EoE和RE患者的miRNA表达模式存在明显差异,EoE病例中有13个miRNA表达增加,10个miRNA表达减少。然后将这些结果与西方报道的EoE患者食管粘膜miRNA表达进行比较,发现表达谱非常相似。此外,在本例EoE患者中,观察到治疗前后几种mirna的表达谱变化。日本EoE患者的miRNA微阵列分析显示,与西方患者相比,miRNA表达明显重叠,表明EoE可能在不同种族人群中代表相同的疾病,并且在世界范围内表现出一致的发病机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
4.30
自引率
8.30%
发文量
57
审稿时长
6-12 weeks
期刊介绍: Journal of Clinical Biochemistry and Nutrition (JCBN) is an international, interdisciplinary publication encompassing chemical, biochemical, physiological, pathological, toxicological and medical approaches to research on lipid peroxidation, free radicals, oxidative stress and nutrition. The Journal welcomes original contributions dealing with all aspects of clinical biochemistry and clinical nutrition including both in vitro and in vivo studies.
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