Journal of Clinical Biochemistry and Nutrition最新文献_第7页

The role of nutrition and oxidative stress as aging factors in Caenorhabditis elegans. 营养和氧化应激在秀丽隐杆线虫衰老因子中的作用。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-11-01 Epub Date: 2023-07-06 DOI: 10.3164/jcbn.23-44

Kayo Yasuda, Masaki Miyazawa, Takamasa Ishii, Naoaki Ishii

引用次数: 0

Response to the Letter. 对信的回应。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-11-01 DOI: 10.3164/jcbn.23-72R

Hidekazu Arai

引用次数: 0

Dietary phosphate disturbs of gut microbiome in mice. 膳食磷酸盐对小鼠肠道微生物群的干扰。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-11-01 Epub Date: 2023-07-15 DOI: 10.3164/jcbn.23-9

Naoko Oda, Kohei Sugihara, Takashi Uebanso, Hirokazu Ohminami, Kohta Ohnishi, Masashi Masuda, Hisami Yamanaka-Okumura, Yutaka Taketani

{"title":"Dietary phosphate disturbs of gut microbiome in mice.","authors":"Naoko Oda, Kohei Sugihara, Takashi Uebanso, Hirokazu Ohminami, Kohta Ohnishi, Masashi Masuda, Hisami Yamanaka-Okumura, Yutaka Taketani","doi":"10.3164/jcbn.23-9","DOIUrl":"10.3164/jcbn.23-9","url":null,"abstract":"Disorder of phosphate metabolism is a common pathological condition in chronic kidney disease patients. Excessive intake of dietary phosphate deteriorates chronic kidney disease and various complications including cardiovascular and infectious diseases. Recent reports have demonstrated that gut microbiome disturbance is associated with both the etiology and progression of chronic kidney disease. However, the relationship between dietary phosphate and gut microbiome remains unknown. Here, we examined the effects of excessive intake of phosphate on gut microbiome. Five-week-old male C57BL/6J mice were fed either control diet or high phosphate diet for eight weeks. Analysis of the gut microbiota was carried out using MiSeq next generation sequencer, and short-chain fatty acids were determined with GC-MS. In analysis of gut microbiota, significantly increased in Erysipelotrichaceae and decreased in Ruminococcaceae were observed in high phosphate diet group. Furthermore, high phosphate diet induced reduction of microbial diversity and decreased mRNA levels of colonic tight junction markers. These results suggest that the excessive intake of dietary phosphate disturbs gut microbiota and affects intestinal barrier function.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"1 1","pages":"221-227"},"PeriodicalIF":2.4,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636580/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69361814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The significance of CDT1 expression in non-cancerous and cancerous liver in cases with hepatocellular carcinoma. CDT1在肝细胞癌非癌肝和癌肝中表达的意义。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-11-01 Epub Date: 2023-08-11 DOI: 10.3164/jcbn.23-43

Masahiro Ogawa, Mitsuhiko Moriyama, Yutaka Midorikawa, Hitomi Nakamura, Toshikatu Shibata, Kazumichi Kuroda, Hisashi Nakayama, Kazunori Kanemaru, Toshio Miki, Masahiko Sugitani, Tadatoshi Takayama

{"title":"The significance of CDT1 expression in non-cancerous and cancerous liver in cases with hepatocellular carcinoma.","authors":"Masahiro Ogawa, Mitsuhiko Moriyama, Yutaka Midorikawa, Hitomi Nakamura, Toshikatu Shibata, Kazumichi Kuroda, Hisashi Nakayama, Kazunori Kanemaru, Toshio Miki, Masahiko Sugitani, Tadatoshi Takayama","doi":"10.3164/jcbn.23-43","DOIUrl":"10.3164/jcbn.23-43","url":null,"abstract":"We previously reported that chromatin licensing and DNA replication factor 1 (CDT1) expression was associated with the extent of proliferation of atypical hepatocytes and the time to postoperative recurrence in cases of hepatocellular carcinoma (HCC). This study aimed to clarify the clinical significance or pathogenesis of CDT1 expression in both non-cancerous and cancerous liver in HCC cases, including previously published data. We investigated the association between the expression of CDT1 in non-cancerous or cancerous liver tissues and histologic findings or biochemical examination results in 62 cases. We also examined the dual localization between CDT1 and FbxW7, P57kip2, P53 and c-Myc by confocal laser scanning microscopy. CDT1 mRNA expression was significantly higher in cancerous liver than in non-cancerous liver (p<0.0001). Elevated CDT1 mRNA expression indicates a significantly degree of inflammatory cell infiltration within lobules, along with elevated serum transaminase levels, and hepatic spare decline. CDT1 mRNA was highly expressed in a group of poorly differentiated cancer cells. CDT1 co-localized with P57kip2, Fbwx7, P53 and c-Myc in the nucleus or cytoplasm of hepatocytes and cancer cells. We found that CDT1 mRNA expression could represent the degree of hepatic spare ability and the high carcinogenic state.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"1 1","pages":"234-248"},"PeriodicalIF":2.4,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636575/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69361200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between reactive oxygen species production in neutrophils and liver fibrosis in the general population. 中性粒细胞中活性氧的产生与一般人群肝纤维化之间的关系。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-11-01 Epub Date: 2023-07-25 DOI: 10.3164/jcbn.23-46

Satoshi Sato, Shigeyuki Nakaji, Kaori Sawada, Naoki Akimoto, Tetsuyuki Tateda, Masatoshi Kaizuka, Takafumi Sasada, Hiroki Nomiya, Go Igarashi, Chikara Iino, Daisuke Chinda, Tatsuya Mikami, Hirotake Sakuraba, Shinsaku Fukuda

{"title":"Association between reactive oxygen species production in neutrophils and liver fibrosis in the general population.","authors":"Satoshi Sato, Shigeyuki Nakaji, Kaori Sawada, Naoki Akimoto, Tetsuyuki Tateda, Masatoshi Kaizuka, Takafumi Sasada, Hiroki Nomiya, Go Igarashi, Chikara Iino, Daisuke Chinda, Tatsuya Mikami, Hirotake Sakuraba, Shinsaku Fukuda","doi":"10.3164/jcbn.23-46","DOIUrl":"10.3164/jcbn.23-46","url":null,"abstract":"Fibrosis, induced by reactive oxygen species (ROS) production in neutrophils, has harmful effects on the liver and various other organs. However, little is known about the association between liver fibrosis and ROS levels in neutrophils in the general population. This large-scale epidemiological study aimed to determine the association between liver fibrosis and neutrophil-generated ROS levels according to age and sex in the general population. This cross-sectional study included 1,000 participants from a district health promotion project. Participants were grouped based on sex (male; female) and age (young, <65 years; old, ≥65 years). The four groups were as follows: male, young (n = 289); male, old (n = 100); female, young (n = 425); and female, old (n = 186). Liver fibrosis was assessed using the fibrosis 4 (FIB-4) index, aspartate aminotransferase-to-platelet ratio index (APRI), and non-alcoholic fatty liver disease (NAFLD) fibrosis score (NFS). Basal and stimulated ROS were considered in the analysis. Multiple linear analyses showed (1) significant positive correlations between all liver fibrosis scores and basal ROS in the young groups, and (2) significant negative correlations between NFS and stimulated ROS in females. Preventing liver fibrosis through neutrophil-related immune system enhancement may avert the development of lifestyle-related diseases and infections.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"1 1","pages":"214-220"},"PeriodicalIF":2.4,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69361264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of lysophosphatidic acid molecules with liver fibrosis: different roles indicated. 溶血磷脂酸分子与肝纤维化的关系:不同的作用表明。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-11-01 Epub Date: 2023-08-18 DOI: 10.3164/jcbn.23-58

Hiroshi Tobita, Hiromichi Sakai, Akane Yamaguchi, Yoshitomo Notsu, Masatoshi Kataoka, Tomotaka Yazaki, Toru Nabika, Shunji Ishihara, Hironori Kobayashi

{"title":"Association of lysophosphatidic acid molecules with liver fibrosis: different roles indicated.","authors":"Hiroshi Tobita, Hiromichi Sakai, Akane Yamaguchi, Yoshitomo Notsu, Masatoshi Kataoka, Tomotaka Yazaki, Toru Nabika, Shunji Ishihara, Hironori Kobayashi","doi":"10.3164/jcbn.23-58","DOIUrl":"10.3164/jcbn.23-58","url":null,"abstract":"Lysophosphatidic acid is composed of lysophosphatidic acid (LPA) molecules with varied chemical forms. The present cross-sectional study was conducted to investigate the associations of various LPA molecules with liver fibrosis. Forty-six patients affected by various types of liver disease who underwent an ultrasound-guided liver biopsy were recruited for this study. Liver fibrosis was evaluated using histological grading, as well as shear wave velocity (Vs) and serum level of type IV collagen 7S (T4c7s). Serum levels of LPA molecules were determined using liquid-chromatography tandem mass-spectrometry (LC-MSMS). Total LPA showed a significant positive association with fibrosis severity evaluated based on histological grading, Vs, and T4c7s used as parameters, following adjustment for other confounding factors, including disease type, age, gender, body mass index, and high-sensitivity C-reactive protein. This association was replicated when 16:0-LPA was substituted for total LPA. In contrast, when 20:4-LPA was substituted for total LPA, no significant association with liver fibrosis was observed. In conclusion, the degree of association varied among the different LPA molecule chemical forms, suggesting different pathophysiological roles of individual LPA molecules, although total LPA concentration was shown to be associated with liver fibrosis.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"1 1","pages":"255-261"},"PeriodicalIF":2.4,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69361756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acute effect of proprotein convertase subtilisin/kexin type 9 inhibitor on oxidized low-density lipoprotein and lipid profile in patients at cardiovascular risk. 蛋白转化酶枯草杆菌素/kexin 9型抑制剂对心血管危险患者氧化低密度脂蛋白和血脂的急性影响

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-11-01 Epub Date: 2023-09-01 DOI: 10.3164/jcbn.23-45

Yiming Li, Minni Sun, Ran Li, Min Dou, Haozhe Dong, Liqi Xue, Guoju Sun

{"title":"Acute effect of proprotein convertase subtilisin/kexin type 9 inhibitor on oxidized low-density lipoprotein and lipid profile in patients at cardiovascular risk.","authors":"Yiming Li, Minni Sun, Ran Li, Min Dou, Haozhe Dong, Liqi Xue, Guoju Sun","doi":"10.3164/jcbn.23-45","DOIUrl":"10.3164/jcbn.23-45","url":null,"abstract":"Proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitors are a new class of potent lipid-lowering drugs. Oxidized low-density lipoprotein (ox-LDL) is the key pathogenic factor leading to atherosclerosis. However, its effect on ox-LDL levels has not been clinically reported. The clinical data of 290 very high-risk atherosclerotic cardiovascular disease (ASCVD) patients diagnosed in the First Affiliated Hospital of Zhengzhou University from May 2022 to October 2022 were collected retrospectively. According to whether evolocumab (a PCSK9 inhibitor) was used after percutaneous coronary intervention (PCI), they were divided into evolocumab group (153 cases) and statin monotherapy group (137 cases). At hospital admission, ox-LDL, total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), apolipoproteinA1 (apoA1), apolipoprotein B-100 (apoB), lipoprotein (a) [Lp(a)], and high-sensitivity reactive protein (hs-CRP) levels were collected and used as baseline data. After two weeks of treatment, ox-LDL in the evolocumab group and statin monotherapy group were significantly lower than those before treatment (p<0.05). The decrease of ox-LDL in the evolocumab group was more than in the stain monotherapy group (p<0.05). In conclusion, PCSK9 inhibitors reduce ox-LDL levels in very high-risk ASCVD patients in a short time.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"1 1","pages":"249-254"},"PeriodicalIF":2.4,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10636578/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69361258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Letter to the Editor: Increased uric acid levels following fructose consumption: a biochemical perspective. 致编辑的信:果糖摄入后尿酸水平升高:生化角度。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-11-01 DOI: 10.3164/jcbn.23-72L

Sarita Anil Shinde

引用次数: 0

TRIM62 knockdown by inhibiting the TLR4/NF-κB pathway and NLRP3 inflammasome attenuates cognitive impairment induced by diabetes in mice. 通过抑制TLR4/NF-κB通路和NLRP3炎性体，敲低TRIM62可减轻糖尿病小鼠认知功能障碍。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-09-01 DOI: 10.3164/jcbn.22-104

Xiting Nong, Nan Li, Xiang Wang, Heng Li, Xiaoping Wu, Ming Li, Wenqing Hao, Guang Yang

{"title":"TRIM62 knockdown by inhibiting the TLR4/NF-κB pathway and NLRP3 inflammasome attenuates cognitive impairment induced by diabetes in mice.","authors":"Xiting Nong, Nan Li, Xiang Wang, Heng Li, Xiaoping Wu, Ming Li, Wenqing Hao, Guang Yang","doi":"10.3164/jcbn.22-104","DOIUrl":"https://doi.org/10.3164/jcbn.22-104","url":null,"abstract":"The tripartite motif 62 is an E3 ubiquitin ligase protein that regulates cellular processes, including differentiation, immunity, development and apoptosis, leading to various disease states, such as cancer and inflammatory diseases. However, the role and mechanism of the tripartite motif 62 in the process of diabetic-induced cognitive impairment have not been reported. Therefore, the aim of this study was to investigate the role and mechanism of the tripartite motif 62 in diabetic-induced cognitive impairment. The results showed that the expression of the tripartite motif 62 was up-regulated in diabetic mice. Silencing of TRIM62 increased body weight and decreased fasting blood glucose in diabetic mice. In addition, knockdown of the tripartite motif 62 inhibited STZ-induced inflammation, apoptosis and oxidative stress. Further studies showed that the TLR4/NF-κB pathway and NLRP3 inflammasomes were involved in the regulation of diabetic mice by the tripartite motif 62. More importantly, inhibition of the tripartite motif 62 improved cognitive impairment and learning ability in mice. In conclusion, inhibition of TRIM62 inhibits STZ-induced inflammation, cell apoptosis and oxidative stress, and improves the cognitive ability of mice. Therefore, the tripartite motif 62 may be an important target for the treatment of diabetes-induced cognitive impairment.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"73 2","pages":"131-137"},"PeriodicalIF":2.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a4/77/jcbn22-104.PMC10493211.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10606767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Involvement of proliferation of atypical hepatocytes and CDT 1 in the liver cancer of rats administered the diethylnitrosamine. 二乙基亚硝胺对肝癌大鼠非典型肝细胞和cdt1增殖的影响。

IF 2.4 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-09-01 DOI: 10.3164/jcbn.13-16

Masahiro Ogawa, Ryota Masuzaki, Tatsuo Kanda, Hiroshi Matsumura, Hitomi Nakamura, Motomi Yamazaki, Toshikatu Shibata, Hirofumi Kogure, Mitsuhiko Moriyama

{"title":"Involvement of proliferation of atypical hepatocytes and CDT 1 in the liver cancer of rats administered the diethylnitrosamine.","authors":"Masahiro Ogawa, Ryota Masuzaki, Tatsuo Kanda, Hiroshi Matsumura, Hitomi Nakamura, Motomi Yamazaki, Toshikatu Shibata, Hirofumi Kogure, Mitsuhiko Moriyama","doi":"10.3164/jcbn.13-16","DOIUrl":"https://doi.org/10.3164/jcbn.13-16","url":null,"abstract":"We have reported that extent of proliferation of atypical hepatocytes (POAH) in non-cancerous liver in hepatocellular carcinoma and chromatin licensing and DNA replication factor 1 (CDT1) are associated with postoperative recurrence. Here, we investigated whether extent of POAH and expression of CDT1 in liver are also associated with chemically induced liver cancer in rats. Male Fisher strain rats were orally administered diethylnitrosamine (DEN) in their drinking water and sacrificed at 6, 8, 12, or 14 weeks after start of DEN administration. We serially monitored changes in extent of POAH, CDT1 expression by immunohistochemistry (IHC), and CDT1 mRNA expression in liver by real-time quantitative PCR. The extent of POAH in liver progressed in a time-dependent manner after start of DEN administration. CDT1 expression was higher at 8 weeks than at 6 weeks by IHC, suggesting that CDT1 expression may be a marker of POAH severity. CDT1 mRNA expression in liver was significantly higher at 12 weeks than at 6 weeks (p<0.0001). We found that extent of POAH and the expression of CDT1 are also important factors in the development of chemical carcinogen-induced hepatocarcinogenesis. Furthermore, the association with POAH and CDT1 expression in carcinogenic process is important regardless of the cause of hepatocarcinogenesis.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"73 2","pages":"138-144"},"PeriodicalIF":2.4,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/60/f1/jcbn13-16.PMC10493214.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10240627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1