Journal of Clinical Biochemistry and Nutrition最新文献_第7页

Effect of chronic kidney disease and type 2 diabetes on plasma branched-chain amino acid cross-correlation: a novel metric for evaluating homeostasis. 慢性肾病和2型糖尿病对血浆支链氨基酸相互关系的影响：一种评估体内平衡的新指标

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-11-01 Epub Date: 2025-06-21 DOI: 10.3164/jcbn.25-47

Hideki Ikeda

{"title":"Effect of chronic kidney disease and type 2 diabetes on plasma branched-chain amino acid cross-correlation: a novel metric for evaluating homeostasis.","authors":"Hideki Ikeda","doi":"10.3164/jcbn.25-47","DOIUrl":"https://doi.org/10.3164/jcbn.25-47","url":null,"abstract":"Cross-correlations between plasma isoleucine (Ile), leucine (Leu), and valine (Val) concentrations are observed in healthy individuals (C group). A parallel shift in these correlations has been noted in patients with end-stage renal failure. To evaluate this shift, a novel b value metric is proposed to assess variations in individuals. Branched-chain amino acids (BCAAs) concentrations were analyzed in four groups: 69 healthy controls (C group), 97 patients with type 2 diabetes mellitus (T2DM), 63 patients with chronic kidney disease (CKD) not on hemodialysis (CKD), and 45 patients with CKD on hemodialysis (CKD-HD). The b value was calculated using the equation Y = aX + b, where coefficient a was derived from the regression line in the C, and each BCAA pair (X, Y) was substituted accordingly. The T2DM exhibited elevated BCAA levels; however, the b values for the (Ile, Leu) and (Ile, Val) pairs remained unchanged. The CKD and CKD-HD showed significant decreases in b values. In T2DM, increased BCAA levels followed the regression line observed in healthy individuals, whereas in CKD, decreased BCAA levels deviated from this pattern. The b value effectively detects changes in BCAA metabolism that are not captured by individual BCAA levels alone, serving as a novel indicator of altered BCAA metabolism and homeostasis.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"77 3","pages":"257-263"},"PeriodicalIF":1.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12646843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145633657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxidative modification of methionine residue in α-synuclein by dopamine induces cellular vulnerability, oligomerization and secretion of α-synuclein. 多巴胺对α-synuclein中蛋氨酸残基的氧化修饰导致细胞易感性、α-synuclein的寡聚和分泌。

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-11-01 Epub Date: 2025-08-20 DOI: 10.3164/jcbn.25-74

Yugo Kato, Masahiro Kanatani, Tadashi Adachi, Hidetoshi Nagamine, Yosuke Horikoshi, Kazuhiro Nakaso

{"title":"Oxidative modification of methionine residue in α-synuclein by dopamine induces cellular vulnerability, oligomerization and secretion of α-synuclein.","authors":"Yugo Kato, Masahiro Kanatani, Tadashi Adachi, Hidetoshi Nagamine, Yosuke Horikoshi, Kazuhiro Nakaso","doi":"10.3164/jcbn.25-74","DOIUrl":"https://doi.org/10.3164/jcbn.25-74","url":null,"abstract":"Parkinson's disease (PD) is a neurodegenerative disorder characterized by the selective loss of dopamine (DA) neurons and presence of Lewy bodies, with prion-like propagation of α-synuclein (α-syn) also attracting attention recently. However, the specific causes for PD-related pathogenesis, including cell vulnerability and α-syn propagation, occurring only in selective neurons remain unclear. Therefore, we aimed to investigate the interactions between DA and α-syn protein to clarify its effects on α-syn degradation, secretion, and toxicity. We generated PC12 cells expressing human α-syn and M127A mutant in a tetracycline-inducible manner. In these cells, intracellular α-syn levels were controlled via autophagic/lysosomal degradation and secretion to extracellular space. Notably, M127A mutation decreased the intracellular degradation and secretion of α-syn. Using the generated cells, we investigated the association between cell viability and oxidized methionine [Met(O)] in α-syn. We also investigated the effects of Met(O) on α-syn toxicity and stability upon DA induction. Wildtype α-syn overexpression decreased the cell viability, and inhibition of methionine sulfoxide reductase, a methionine sulfoxide-reducing enzyme, further amplified this effect, suggesting that α-syn cytotoxicity is associated with methionine oxidation. Notably, vulnerabilities of M127A mutant cells were lower than those of wildtype α-syn-expressing cells. Overall, our results suggest M127 as the major target for oxidative modification by DA and that this modification is associated with both cell vulnerability and α-syn intracellular stability and secretion in PD pathogenesis.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"77 3","pages":"216-222"},"PeriodicalIF":1.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12646842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145633797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting Helicobacter pylori in liver-impaired populations: a systematic review of efficacy and safety. 针对肝受损人群的幽门螺杆菌：疗效和安全性的系统评价。

IF 1.9 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-11-01 Epub Date: 2025-07-02 DOI: 10.3164/jcbn.25-68

Hideki Mori, Chikara Iino, Takeshi Fujieda

{"title":"Targeting Helicobacter pylori in liver-impaired populations: a systematic review of efficacy and safety.","authors":"Hideki Mori, Chikara Iino, Takeshi Fujieda","doi":"10.3164/jcbn.25-68","DOIUrl":"10.3164/jcbn.25-68","url":null,"abstract":"Liver impairment can alter the metabolic capacity of the organ significantly, potentially affecting the efficacy and safety of Helicobacter pylori (H. pylori) eradication regimens. Although this issue is highly significant, no standardized guidelines currently exist for H. pylori eradication therapy in patients with liver dysfunction. To bridge this gap, we systematically reviewed studies on the efficacy and safety of H. pylori eradication therapy in individuals with liver impairment. The systematic review included a search of PubMed, the Igaku Chuo Zasshi, and the Cochrane Library database to identify eligible randomized controlled trials (RCTs) and/or non-randomized controlled trials (NRCTs). Three RCTs and five NRCTs were included. Research on the effectiveness of H. pylori eradication treatment in patients with and without liver disease has produced inconsistent findings, with a study reporting higher eradication rates in the liver disease group, and others finding no significant difference, leaving no definitive consensus. Two studies reported eradication rates <70% in patients with HCV infection, whereas other studies demonstrated eradication rates >80% in groups with liver impairment. Furthermore, even in cases of advanced liver impairment such as non-alcoholic steatohepatitis (NASH) or liver cirrhosis, eradication rates did not decline, and adverse events were either minimal or comparable with those observed in groups without liver impairment. H. pylori eradication therapy is applicable and safe for patients with liver disease; however, treatment indications should be determined carefully by considering reduced hepatic metabolic capacity and specific indications for eradication, while remaining vigilant for potential adverse effects.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"77 3","pages":"203-208"},"PeriodicalIF":1.9,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12646836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145633831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of autonomic function in patients with functional dyspepsia using real-time wearable devices and smartphone applications. 利用实时可穿戴设备和智能手机应用分析功能性消化不良患者的自主神经功能。

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-11-01 Epub Date: 2025-07-03 DOI: 10.3164/jcbn.24-180

Fumio Tanaka, Rieko Nakata, Noriaki Sugawara, Kazuhide Higuchi, Yasuhiro Fujiwara

{"title":"Analysis of autonomic function in patients with functional dyspepsia using real-time wearable devices and smartphone applications.","authors":"Fumio Tanaka, Rieko Nakata, Noriaki Sugawara, Kazuhide Higuchi, Yasuhiro Fujiwara","doi":"10.3164/jcbn.24-180","DOIUrl":"https://doi.org/10.3164/jcbn.24-180","url":null,"abstract":"Autonomic dysfunction is involved in functional dyspepsia (FD) however, the details have not been elucidated. The aim is to clarify the relationship among autonomic nervous system (ANS) activity, life events, and abdominal symptoms by real-time recordings. This is a prospective multicenter study including 9 patients with FD and 23 healthy controls (HC). The ANS activity was recorded for 24 ‍h using a T-shirt-type wearable device. Life events and abdominal symptoms were simultaneously recorded with ANS activity by smartphone application software. We defined low-frequency/high-frequency (LF/HF) and HF as activity indicators of the sympathetic and parasympathetic nerves, respectively. The number of abnormal LF/HF and HF in patients with FD was significantly higher than that in HC. The number of abnormal LF/HF and HF signals was significantly positively correlated with dyspeptic symptoms. The number of abnormal LF/HF signals was significantly negatively correlated with quality of life (QOL). During the symptomatic period, the HF levels were elevated in the HC group. In contrast, HF significantly decreased in patients with FD, which may imply parasympathetic nervous system dysfunction. In conclusion, patients with FD have abnormal ANS activity, which is associated with symptoms and lower QOL, and they exhibit parasympathetic nervous system dysfunction during symptomatic period.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"77 3","pages":"249-256"},"PeriodicalIF":1.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12646847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145633993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the nutritional applications of therapeutic diet for swallowing disorders at different IDDSI levels. 不同IDDSI水平下吞咽障碍治疗性饮食的营养应用研究。

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-11-01 Epub Date: 2025-09-10 DOI: 10.3164/jcbn.25-116

Yi Cheng, Muxi Chen, Ke Li, Jiuming Yan, Wen Hu, Lei Shi, Yuan Liu

{"title":"Investigation of the nutritional applications of therapeutic diet for swallowing disorders at different IDDSI levels.","authors":"Yi Cheng, Muxi Chen, Ke Li, Jiuming Yan, Wen Hu, Lei Shi, Yuan Liu","doi":"10.3164/jcbn.25-116","DOIUrl":"https://doi.org/10.3164/jcbn.25-116","url":null,"abstract":"This study investigates the nutritional applicability of balanced therapeutic diets for elderly individuals (aged 65 and above) with dysphagia, using the International Dysphagia Diet Standardization Initiative (IDDSI) and the 2022 Chinese Dietary Guidelines as dual frameworks. Ingredient data were collected from \"Meishichina\", a Chinese culinary database, and processed into dishes complying with IDDSI levels 1-7. Nutritional values were calculated using West China Hospital Nutrition Software, and statistical analyses, including ANOVA and Spearman correlation, were conducted to compare nutrients across IDDSI levels. Results showed significant variation in protein, fat, and carbohydrate content among food categories and IDDSI levels (p≤0.05), with meats and eggs contributing mainly to protein and fat, while fruits and grains contributed carbohydrates. Spearman analysis revealed positive correlations between IDDSI levels and energy, protein, and fat intake (p≤0.05). Only IDDSI level 7 met the elderly-specific target of 1,000 ‍kcal and 60 ‍g protein per meal. To address the nutritional gaps in other levels, supplementation with \"Yi Quan Su\" enteral nutrition powder was calculated based on the deficits. These findings highlight the nutritional limitations of texture-modified diets at lower IDDSI levels and emphasize the importance of targeted supplementation to meet the needs of older adults with dysphagia.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"77 3","pages":"268-279"},"PeriodicalIF":1.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12646846/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145633669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adiponectin inhibits chemokine-induced cell migration via direct interaction with platelet factor 4. 脂联素通过与血小板因子4的直接相互作用抑制趋化因子诱导的细胞迁移。

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-09-01 Epub Date: 2025-03-27 DOI: 10.3164/jcbn.24-230

Mohamed Elfeky, Shima Goto, Ali El-Far, Yuko Okamatu-Ogura, Kazuhiro Kimura

引用次数: 0

Regulating effect of serum selenium on diabetic kidney disease related all-cause mortality: evidence from NHANES database. 血清硒对糖尿病肾病相关全因死亡率的调节作用：来自NHANES数据库的证据

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-09-01 Epub Date: 2025-04-05 DOI: 10.3164/jcbn.25-25

Ruolan Lin, Yurong Xian, Miaoling Tan, Guoqin Liu

{"title":"Regulating effect of serum selenium on diabetic kidney disease related all-cause mortality: evidence from NHANES database.","authors":"Ruolan Lin, Yurong Xian, Miaoling Tan, Guoqin Liu","doi":"10.3164/jcbn.25-25","DOIUrl":"10.3164/jcbn.25-25","url":null,"abstract":"To explore the interaction between Diabetic kidney disease (DKD) and serum selenium (Se) on mortality from all-cause. Information from the National Health and Nutrition Examination Surveys database (2011-2016) were retrospectively extracted for this cohort study. Associations of DKD and serum Se level with all-cause mortality were evaluated by weighted uni- and multi-variate COX regression analyses, with 95% confidence intervals (CIs) and hazard ratios (HRs). The interaction was measured by attributable proportion of interaction (APAB), relative excess risk due to interaction (RERI), synergy index (S) and 95% CIs. Among 793 adult patients, 98 died from all cause. DKD was linked to an increased all-cause mortality risk [HR = 3.69, 95% CI (1.75-7.81)], while elevated serum Se levels were linked to a decreased all-cause mortality risk [HR = 0.49, 95% CI (0.28-0.86)], after adjusting for co-variables including age, education level, physical activity (PA), CVD, WBC, neutrophil, HB, ALB, and Zn. Additionally, there was a potential antagonistic interaction between DKD and serum Se level on all-cause mortality, with the S value of 0.228. Se may play a protective role in all-cause mortality related to DKD in patients with DM, but this interaction effect needed further exploration.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"77 2","pages":"182-188"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12440675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reduced phase angle as a potential indicator of mild cognitive impairment. 相角减小作为轻度认知障碍的潜在指标。

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-09-01 Epub Date: 2025-05-22 DOI: 10.3164/jcbn.25-70

Mika Hasegawa, Hidenori Onishi, Yasutaka Mizukami, Yuki Niida, Tomoko Okamoto, Masafumi Kubota, Yuya Nakajima, Taisei Inoue, Hirohiko Ohama, Tokuharu Tanaka, Shinya Sugawara, Fumie Maeda, Akemi Koujimoto, Yuta Shimoura, Osamu Muto, Naohiro Konoshita, Akiko Matsunaga, Masamichi Ikawa, Hiroyuki Hayashi, Osamu Yamamura

{"title":"Reduced phase angle as a potential indicator of mild cognitive impairment.","authors":"Mika Hasegawa, Hidenori Onishi, Yasutaka Mizukami, Yuki Niida, Tomoko Okamoto, Masafumi Kubota, Yuya Nakajima, Taisei Inoue, Hirohiko Ohama, Tokuharu Tanaka, Shinya Sugawara, Fumie Maeda, Akemi Koujimoto, Yuta Shimoura, Osamu Muto, Naohiro Konoshita, Akiko Matsunaga, Masamichi Ikawa, Hiroyuki Hayashi, Osamu Yamamura","doi":"10.3164/jcbn.25-70","DOIUrl":"10.3164/jcbn.25-70","url":null,"abstract":"In this study, we investigated the relationship between phase angle (PhA), measured using bioelectrical impedance analysis (BIA), and mild cognitive impairment (MCI). A cross-sectional analysis was conducted on 290 community residents (83.7% female, average age 74.9 years). Cognitive function was assessed using the Japanese version of the Montreal Cognitive Assessment (MoCA-J), with MCI defined as a score of ≤25. Body composition, including PhA, was measured using BIA. Multiple regression analysis was used to examine the association between PhA and MCI presence, adjusting for potential confounders. MCI was found in 168 participants. The PhA (leg) was significantly lower in those with MCI than in those without (p = 0.013). A significant association between leg PhA and MCI was identified in the regression model (β = 0.103, p = 0.015), with an adjusted R 2 value of 0.50. These findings suggested that PhA may serve as an indicator of MCI. Longitudinal and intervention studies are needed to explore the potential of PhA in dementia prevention strategies. In addition, future research should focus on developing dementia prevention strategies that utilize PhA through longitudinal and interventional studies.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"77 2","pages":"189-194"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12440668/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mallotus japonicus extract suppresses ferroptosis by inhibiting transferrin receptor-mediated ferrous ion uptake in human tubular epithelial HK2 cells. 马蹄莲提取物通过抑制人小管上皮HK2细胞转铁蛋白受体介导的铁离子摄取来抑制铁凋亡。

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-09-01 Epub Date: 2025-05-14 DOI: 10.3164/jcbn.25-42

Tetsuro Kamiya, Misaki Nishimura, Tomohiro Otsuka, Hiroki Yoshinaka, Hiroe Maruyama, Hiroyuki Kono, Hirokazu Hara

{"title":"Mallotus japonicus extract suppresses ferroptosis by inhibiting transferrin receptor-mediated ferrous ion uptake in human tubular epithelial HK2 cells.","authors":"Tetsuro Kamiya, Misaki Nishimura, Tomohiro Otsuka, Hiroki Yoshinaka, Hiroe Maruyama, Hiroyuki Kono, Hirokazu Hara","doi":"10.3164/jcbn.25-42","DOIUrl":"10.3164/jcbn.25-42","url":null,"abstract":"Iron (Fe) is the most abundant metal ion in the body, but its excess accumulation causes Fe2+-dependent and lipid peroxidation-dependent cell death (ferroptosis) via the production of reactive oxygen species (ROS). It is thought that ferroptosis is related to the progression of various kidney problems, including acute kidney injury and diabetic nephropathy. Mallotus japonicus (M. japonicus), a deciduous shrub belonging to the Euphorbiaceae family, contains ellagitannins such as corilagin, geraniin, mallotinic acid, and mallotusinic acid, which have antioxidant properties. Here, we investigated whether M. japonicus leaf extract inhibits ferroptosis in human proximal tubular epithelial HK2 cells. M. japonicus extract suppressed HK2 cell death caused by erastin, a ferroptosis inducer; this was accompanied by a reduction in intracellular Fe2+, ROS accumulation, and lipid peroxidation. Our findings suggested that the inhibitory effect of M. japonicus extract was due to the inhibition of transferrin receptor (CD71)-mediated Fe3+/2+ uptake. Furthermore, we determined that mallotinic acid is a key compound that exhibits anti-ferroptosis effects. Overall, our results provide useful information for the use of M. japonicus extract in the treatment of kidney injuries.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"77 2","pages":"153-161"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12440676/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell sequencing analysis reveals the promotion of ELR CXCL genes on angiogenesis and the influence on malignant progression in glioblastoma. 单细胞测序分析揭示了ELR CXCL基因对胶质母细胞瘤血管生成的促进作用和对恶性进展的影响。

IF 1.7 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2025-09-01 Epub Date: 2025-05-28 DOI: 10.3164/jcbn.25-26

Fanyong Gong, Yi Huang, Junjun Zhang, Jianfei Zhang, Haifeng Wang

{"title":"Single-cell sequencing analysis reveals the promotion of ELR CXCL genes on angiogenesis and the influence on malignant progression in glioblastoma.","authors":"Fanyong Gong, Yi Huang, Junjun Zhang, Jianfei Zhang, Haifeng Wang","doi":"10.3164/jcbn.25-26","DOIUrl":"10.3164/jcbn.25-26","url":null,"abstract":"Glioblastoma (GBM) is a highly aggressive cancer that significantly impacts human health. Myeloid cells and bone marrow-derived macrophages (BMDMs) are major components of tumor microenvironment; however, their roles in GBM tumorigenesis, progression, and treatment response remain unclear due to considerable heterogeneity. Single-cell sequencing data for GBM were collected from the Gene Expression Omnibus (GEO) database to analyze the heterogeneity of GBM cell subpopulations. Trends in myeloid cell subpopulations were examined, identifying cancer-associated BMDMs and key genes that were specifically overexpressed in this subgroup. Validation was performed through experiments in vitro, including quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) to measure mRNA expressions of chemokine (C-X-C motif) ligand 3 (CXCL3), vascular endothelial growth factor A (VEGF-A), and matrix metallopeptidase 9 (MMP9); angiogenesis assays to observe endothelial cell tube formation; Cell Counting Kit-8 (CCK-8) assays to assess cell viability; colony formation assays for cell proliferation; Transwell assays to evaluate cell migration and invasion; and flow cytometry to measure apoptosis. Eight distinct cell types were identified in GBM, with a notable proportion of myeloid cells. Cancer-related BMDMs were characterized within the myeloid cell population, revealing specific overexpression of Glu-Leu-Arg (ELR) CXCL genes associated with angiogenesis and tumor development. Experiments in vitro confirmed that the ELR CXCL-related gene CXCL3 derived from BMDMs promoted angiogenesis and influenced GBM malignancy. The ELR CXCL + BMDM subgroup represented a critical cell type associated with the rapid growth of GBM, as the secreted CXCL3 enhanced angiogenesis and impacted GBM malignant progression.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"77 2","pages":"144-152"},"PeriodicalIF":1.7,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12440671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145080950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0