Effect of fat ingestion on postprandial oxidative status in healthy young women: a pilot study.

IF 2 4区 医学 Q3 NUTRITION & DIETETICS
Journal of Clinical Biochemistry and Nutrition Pub Date : 2024-01-01 Epub Date: 2023-08-09 DOI:10.3164/jcbn.23-50
Kozo Takeuchi, Kimiko Kazumura, Kaori Kuzawa, Yukiko Hatano, Masashi Nagai, Michitaka Naito
{"title":"Effect of fat ingestion on postprandial oxidative status in healthy young women: a pilot study.","authors":"Kozo Takeuchi, Kimiko Kazumura, Kaori Kuzawa, Yukiko Hatano, Masashi Nagai, Michitaka Naito","doi":"10.3164/jcbn.23-50","DOIUrl":null,"url":null,"abstract":"<p><p>Reactive oxygen species (ROS) and highly reactive oxygen species (hROS) secreted by leukocytes are crucial to innate immunity; however, they pose a risk of oxidative stress. To monitor their balance in daily health check-ups, optical technologies for the simultaneous measurement of ROS (superoxide radicals) and hROS (hypochlorite ions) that utilize only a few microliters of whole blood have been developed. The aim of this study was to clarify whether this system could assess the effects of fat ingestion on postprandial oxidative status. Eight healthy young Japanese women ingested a beverage containing oral fat tolerance test cream. Blood samples were collected before and 0.5, 1, 2, 4, and 6 h after fat ingestion. Blood ROS and hROS levels, oxidative stress markers, and biochemical markers were monitored. Consistent with previous studies, triglyceride levels significantly increased at 4 h (<i>p</i><0.01) and returned to near-baseline levels 6 h after ingestion. ROS levels peaked significantly at 2 h (<i>p</i><0.05), and hROS levels peaked significantly at 1 (<i>p</i><0.05) and 2 h (<i>p</i><0.01) after ingestion. This study offers an insight into the acute effects of fat ingestion on leukocyte activity and provides a methodology for monitoring postprandial oxidative status.</p>","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"1 1","pages":"30-36"},"PeriodicalIF":2.0000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10822754/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Clinical Biochemistry and Nutrition","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3164/jcbn.23-50","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/8/9 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"NUTRITION & DIETETICS","Score":null,"Total":0}
引用次数: 0

Abstract

Reactive oxygen species (ROS) and highly reactive oxygen species (hROS) secreted by leukocytes are crucial to innate immunity; however, they pose a risk of oxidative stress. To monitor their balance in daily health check-ups, optical technologies for the simultaneous measurement of ROS (superoxide radicals) and hROS (hypochlorite ions) that utilize only a few microliters of whole blood have been developed. The aim of this study was to clarify whether this system could assess the effects of fat ingestion on postprandial oxidative status. Eight healthy young Japanese women ingested a beverage containing oral fat tolerance test cream. Blood samples were collected before and 0.5, 1, 2, 4, and 6 h after fat ingestion. Blood ROS and hROS levels, oxidative stress markers, and biochemical markers were monitored. Consistent with previous studies, triglyceride levels significantly increased at 4 h (p<0.01) and returned to near-baseline levels 6 h after ingestion. ROS levels peaked significantly at 2 h (p<0.05), and hROS levels peaked significantly at 1 (p<0.05) and 2 h (p<0.01) after ingestion. This study offers an insight into the acute effects of fat ingestion on leukocyte activity and provides a methodology for monitoring postprandial oxidative status.

摄入脂肪对健康年轻女性餐后氧化状态的影响:一项试验研究。
白细胞分泌的活性氧(ROS)和高活性氧(hROS)对先天性免疫至关重要,但它们也带来了氧化应激的风险。为了在日常健康检查中监测它们的平衡,人们开发出了只需几微升全血就能同时测量 ROS(超氧自由基)和 hROS(次氯酸盐离子)的光学技术。本研究的目的是明确该系统能否评估摄入脂肪对餐后氧化状态的影响。八名健康的年轻日本女性摄入了含有口服脂肪耐受性测试膏的饮料。分别在摄入脂肪前、摄入脂肪后 0.5、1、2、4 和 6 小时采集血液样本。对血液中的 ROS 和 hROS 水平、氧化应激标记物和生化标记物进行了监测。与之前的研究一致,甘油三酯水平在 4 小时后明显增加(ppp
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
4.30
自引率
8.30%
发文量
57
审稿时长
6-12 weeks
期刊介绍: Journal of Clinical Biochemistry and Nutrition (JCBN) is an international, interdisciplinary publication encompassing chemical, biochemical, physiological, pathological, toxicological and medical approaches to research on lipid peroxidation, free radicals, oxidative stress and nutrition. The Journal welcomes original contributions dealing with all aspects of clinical biochemistry and clinical nutrition including both in vitro and in vivo studies.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信