Journal of Clinical Biochemistry and Nutrition最新文献

{"title":"Ischemia/reperfusion-associated oxidative stress is an aggravating factor for pressure ulcers.","authors":"Eri Murata, Junichi Fujii","doi":"10.3164/jcbn.25-28","DOIUrl":"10.3164/jcbn.25-28","url":null,"abstract":"<p><p>Prolonged compression restricts blood flow, which can result in an insufficient supply of the oxygen and nutrients that are necessary for cells to live and thrive. A pressure ulcer (PU) is a pathological condition of prolonged ischemia and is associated with a loss of epidermis, which could expose the dermis and deeper tissues. It is necessary to remove the causative pressure and resume blood flow, but injurious damage to the ischemic area could occur with a delay after blood reflow, which could result in reperfusion injury. In the early stages of compression, muscle cells, which are highly dependent on aerobic respiration, are preferably damaged, and then the injury could spread to other dermal and epithelial cells. Infiltrated leukocytes release a variety of substances such as cytokines and reactive oxygen species (ROS) that act as anti-microorganisms, but these could also aggravate inflammation. The ROS are deeply involved in the pathogenesis of this condition, and, hence, antioxidant compounds and enzymes play a vital role in preventing the progression of PUs. We focus on oxidative stress in PUs, which is exacerbated by the actions of ischemia/reperfusion, and we discuss effective prevention and treatment from the perspectives of micronutrients and antioxidants.</p>","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 3","pages":"221-232"},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152245/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat-killed <i>Lactococcus lactis</i> subsp. <i>lactis</i> L8 ameliorates dextran sulfate sodium-induced colitis in mice.","authors":"Shogo Kawaguchi, Daisuke Chinda, Shinji Ota, Akio Tonouchi, Hayato Maeda, Kazuhide Miura, Go Soma, Hiroto Hiraga, Kayo Ueno, Takenori Niioka, Mayuki Tachizaki, Kazuhiko Seya, Tadaatsu Imaizumi, Shinsaku Fukuda, Hirotake Sakuraba","doi":"10.3164/jcbn.24-194","DOIUrl":"10.3164/jcbn.24-194","url":null,"abstract":"<p><p><i>Lactococcus lactis</i> subsp. <i>lactis</i> (<i>L. lactis</i>) is a common species of lactic acid bacteria (LAB). There is increasing evidence that probiotic <i>L. lactis</i> ameliorates experimental colitis in mice, whereas few studies have revealed the effects of the paraprobiotics, i.e., inactivated forms of bacteria. <i>L. lactis</i> L8 strain is a newly identified plant-derived LAB. The present study aimed to investigate the effects of heat-killed <i>L. lactis</i> L8 on dextran sulfate sodium (DSS)-induced colitis in mice. C57BL/6J mice were orally administered <i>L. lactis</i> L8 for 12 days. Colitis was induced by adding 3.5% DSS to the drinking water for 7 days. Mice were euthanized on day 12, and colon tissues and fecal samples were collected. Results demonstrated that administration of <i>L. lactis</i> L8 alleviated the clinical score of the colitis and the histological abnormalities. Additionally, <i>L. lactis</i> L8 led to a significant reduction in the level of colon tumor necrosis factor (TNF)-α. Administration of <i>L. lactis</i> L8 did not affect the gut microbial structure, whereas it altered the relative abundance of <i>Clostridiaceae</i> and <i>Rikenellaceae</i> in colitis mice. Our findings suggest that paraprobiotic <i>L. lactis</i> L8 has an immunomodulatory effect on intestinal inflammation in mice, providing important insight into the biological function of <i>L. lactis</i> L8.</p>","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 3","pages":"264-270"},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of factors associated with osteoporosis using metabolome analysis.","authors":"Madoka Sumi, Takuro Okamura, Shintaro Kajiyama, Tomoki Miyoshi, Naoko Nakanishi, Yoshitaka Hashimoto, Ryoichi Sasano, Masahide Hamaguchi, Michiaki Fukui","doi":"10.3164/jcbn.24-199","DOIUrl":"10.3164/jcbn.24-199","url":null,"abstract":"<p><p>Osteoporosis is associated with low bone mineral density and poor bone quality in patients with type 2 diabetes mellitus (T2DM). Reports on the association between serum metabolome and osteoporosis in diabetes mellitus are rare. We aimed to identify osteoporosis-related factors in patients with T2DM. This prospective observational study included 67 patients with T2DM without osteoporosis and 24 patients with T2DM with osteoporosis who were treated with teriparatide. The association between the serum metabolome at baseline and changes in bone mineral density and the bone quality of the lumbar spine and proximal femur over 1 year was evaluated. In the teriparatide-treated group, bone mineral density, young adult mean values, and trabecular bone scores (T-scores) of the lumbar spine significantly increased 1 year after baseline compared with those at baseline. Improvements in these bone assessment indices were significantly higher in the teriparatide-treated group than in the non-treated group. Baseline alkaline phosphatase, bone-specific alkaline phosphatase, and malonic acid levels were significantly negatively correlated with bone mineral density, young adult mean values, and T-scores. Changes in bone mineral density, young adult mean values, and T-score 1 year after baseline were significantly positively correlated with baseline alkaline phosphatase, bone-specific alkaline phosphatase, oxalic acid, and aspartic acid levels in the teriparatide-treated group. Serum oxalic acid and malonic acid levels may be novel markers of osteoporosis. Teriparatide may be more effective in patients with higher baseline serum oxalic acid and aspartic acid levels than in those with lower levels.</p>","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 3","pages":"304-310"},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is nerve-directed stretching effective for improving of ankle joint flexibility and tissue stiffness in older men?","authors":"Kosuke Hirata, Daiki Watanabe, Toshiaki Soga, Ryota Akagi","doi":"10.3164/jcbn.24-238","DOIUrl":"10.3164/jcbn.24-238","url":null,"abstract":"<p><p>Age-related decrease in joint flexibility leads to difficulties in activities of daily living and an increased risk of falls. The current study investigated whether nerve-directed stretching, which focuses on stretching neural tissue, could acutely improve joint flexibility and tissue stiffness more than muscle-directed stretching or not. Twenty-seven older men performed muscle- and nerve-directed stretching for 400 ‍s (40 s × 10 times) on separate days. Before and after stretching, maximum ankle dorsiflexion angle was measured as range of motion (ROM). Shear wave propagation velocity (SWV) was also assessed as tissue stiffness of the sciatic nerve, medial gastrocnemius, posterior thigh fascia, and posterior leg fascia in anatomical position. In both stretching conditions, increment of ROM and reduction of sciatic nerve SWV were observed after stretching. The extent of these changes did not differ between the stretching conditions. The SWVs of tissues other than the sciatic nerve did not change before and after the stretching. These results suggest that acute effects of nerve-directed stretching on joint flexibility and tissue stiffness are not superior to muscle-directed stretching.</p>","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 3","pages":"233-238"},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152241/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aging-related dysregulation of energy metabolism and mitochondrial dynamics in microglia.","authors":"Yoki Nakamura, Manaya Nakano, Kazue Hisaoka-Nakashima, Norimitsu Morioka","doi":"10.3164/jcbn.24-202","DOIUrl":"10.3164/jcbn.24-202","url":null,"abstract":"<p><p>Microglia, the primary immune cells of the central nervous system, play a pivotal role in maintaining brain homeostasis. Recent studies have highlighted the involvement of microglial dysfunction in the pathogenesis of various age-related neurodegenerative diseases, such as Alzheimer's disease. Moreover, the metabolic state of microglia has emerged as a key factor in these diseases. Interestingly, aging and neurodegenerative diseases are associated with impaired mitochondrial function and a metabolic shift from oxidative phosphorylation to glycolysis in microglia. This metabolic shift may contribute to sustained microglial activation and neuroinflammation. Furthermore, the leakage of mitochondrial DNA into the cytoplasm, because of mitochondrial dysfunction, has been implicated in triggering inflammatory responses and disrupting brain function. This review summarizes recent advances in understanding the role of microglial metabolic shifts, particularly glycolysis, and mitochondrial dysfunction. It also explores the potential of targeting microglial metabolism, for instance by modulating mitophagy or intervening in specific metabolic pathways, as a novel therapeutic approach for changes in brain function and neurodegenerative diseases associated with aging.</p>","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 3","pages":"239-244"},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152248/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nobiletin, a polymethoxylated flavonoid from citrus, enhances IL-4 production in DO11.10 mice via antigen-presenting cells.","authors":"Haruka Ohashi, Akiko Nakamoto, Yuko Tanaka, Moeka Tomonobu, Karen Mori, Mariko Nakamoto, Tohru Sakai","doi":"10.3164/jcbn.24-226","DOIUrl":"10.3164/jcbn.24-226","url":null,"abstract":"<p><p>The polymethoxylated flavonoid nobiletin has been shown to have a broad range of biological functions. We found that nobiletin regulates cytokine production from T cells. In this study, we examined the mechanism for enhancement of interleukin (IL)-4 production by nobiletin. We investigated the key molecules for production of IL-4 by deep data-based analysis and we identified nine candidate genes. The mRNA expression levels of <i>c-Maf</i>, <i>Gata-3</i>, <i>Nfat4</i>, and <i>Pparg</i> genes were significantly elevated by treatment with nobiletin. From RNA sequence analysis, the T helper type 1 (Th1) and Th2 differentiation pathway was shown to be dominantly affected by nobiletin. Furthermore, molecular network analysis showed that GATA-3 is one of the candidate molecules for enhancement of IL-4 production. Treatment of splenocytes including antigen-presenting cells with nobiletin was sufficient for the induction of IL-4 production from DO11.10 mouse CD4⁺ T cells. Treatment of B cells and dendritic cells with nobiletin induced IL-4 production from CD4⁺ cells. Splenocytes from DO11.10 mice that had been treated with nobiletin produced more IL-4 than did splenocytes from control mice. These results revealed that the polymethoxylated flavonoid nobiletin specifically enhanced IL-4 production <i>in vitro</i> and <i>in vivo</i>.</p>","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 3","pages":"245-252"},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of disruption of mitochondrial and endoplasmic reticulum calcium homeostasis on neurites in hydrogen peroxide- and ionomycin-treated cells.","authors":"Kazuki Hoga, Mitsuru Wakuzawa, Tsukasa Nakamura, Yugo Kato, Koji Fukui","doi":"10.3164/jcbn.24-122","DOIUrl":"10.3164/jcbn.24-122","url":null,"abstract":"<p><p>Neurite degeneration is seen in the early stages of many neurodegenerative diseases, and is strongly related to oxidative damage. Possible mechanisms underlying this morphological change include dysruption of calcium homeostasis, increased membrane oxidation, and destabilization of cytoskeletal proteins. However, the detailed mechanisms leading to neuronal damage has not been elucidated. Calcium plays an important role in neuronal changes caused by oxidative stress. Mitochondria and endoplasmic reticulum (ER) play roles in intracellular calcium storages. One mechanism of neurite degeneration associated with oxidative stress may be related to calcium-mediated interactions between mitochondria and ER. In this study, we evaluated intracellular calcium homeostasis, mitochondria, and ER localization when neurite degeneration was induced in neuroblastoma cells that had extended neurites. Treatment with hydrogen peroxide (H₂O₂) and the calcium ionophore ionomycin induced mitochondria-dependent superoxide production and membrane oxidation. When examining the involvment of calcium efflux from the ER and mitochondria, treatment with a ryanodine receptor inhibitor ruthenium red significantly reduced intracellular calcium concentrations in ionomycin-treated cells. Electron microscopy in neurite degeneration areas revealed numerous fragmented mitochondria in ionomycin-treated cells, and mitochondrial swelling was observed in the same area of H₂O₂-treated cells. Next, we investigated proteins related to fusion and fission by western blotting. However, mitochondrial dysfunction occurs in both cases and is therefore thought to be associated with neurite degeneration. Our results suggest that H₂O₂ and ionomycin cause neurite degeneration via different mechanisms. Interactions between mitochondria and the ER through unknown crosstalk pathways and calcium transfer may play an important role in maintaining neurite function.</p>","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 3","pages":"253-263"},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152244/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of vitamin D status on the variability of collagen I C-telopeptide in pre- and post-menopausal healthy women.","authors":"Caterina Saija, Maria Paola Bertuccio, Monica Currò, Daniela Caccamo","doi":"10.3164/jcbn.24-146","DOIUrl":"10.3164/jcbn.24-146","url":null,"abstract":"<p><p>Early predictive markers of bone resorption are required to plan intervention strategies ensuring a healthy aging. Collagen I C-telopeptide (CTX) as bone activity marker shows a fair biological variability, that cause the lack of a well-defined reference range. Given the well-established role of vitamin D in bone remodeling, the aim of this study was to assess the influence of vitamin D status on the variability of CTX serum levels in 131 pre-menopausal (45-54 years) and 951 post-menopausal women (55-90 years). Serum CTX and vitamin D levels were assessed, respectively, by ELISA and HPLC determination of 25-hydroxy-vitamin D3. A significantly negative dependence of CTX on vitamin D3, but not age, was found in the whole study cohort (B = -0.170, <i>p</i><0.0001). A significantly negative correlation between CTX and vitamin D3 was found both in pre-menopausal (r = -0.2614; <i>p</i> = 0.0061) and post-menopausal women (r = -0.2220; <i>p</i><0.0001), and confirmed in post-menopausal women aged 55 to 59 years (r = -0.2840, <i>p</i> = 0.0061), 60 to 64 years (r = -0.2143, <i>p</i> = 0.0129), and 70 to 74 years (r = -0.3078, <i>p</i><0.0001). These findings suggest that vitamin D status determination and early vitamin D supplementation may be required in women at higher risk of bone resorption because of the physiologically reduced protective effects of estrogens.</p>","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 3","pages":"311-316"},"PeriodicalIF":2.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12152249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144284462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"β-Elemene inhibits adipogenesis in 3T3-L1 cells by regulating AMPK pathway.","authors":"Xiang Deng, Zhenmin Liu, Sen Yang","doi":"10.3164/jcbn.24-179","DOIUrl":"10.3164/jcbn.24-179","url":null,"abstract":"<p><p>The prevalence of childhood obesity in global is quickly augmented, resulting into grievous public health problems and influencing adolescent development. β-Elemene is a sesquiterpene, and can extracted from traditional Chinese medicine-<i>Curcuma longa</i> L. β-Elemene has been discovered to display regulatory functions in multiple diseases, but it's roles in obesity need further investigations. The purpose of this work is to investigate the regulatory impacts of β-elemene on obesity progression and associated pathways. In this study, it was revealed that the heightened lipid accumulation in 3T3-L1 cells triggered by 3-isobutyl-1-methylxanthine + dexamethazone + insulin (MDI) can be restrained by β-elemene. Furthermore, β-elemene can modulate lipid metabolism in 3T3-L1 cells mediated by MDI. The glucose consumption was descended after insulin resistance treatment, but this impact was reversed after β-elemene treatment. At last, it was illustrated that the AMPK pathway was retarded after β-elemene induction, but this change was offset after β-elemene treatment. To sum up, our results manifested that β-elemene inhibited adipogenesis in 3T3-L1 cells, and evoked the AMPK pathway. This project may supply serviceable insights of β-elemene in the progression of obesity.</p>","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 2","pages":"125-130"},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936742/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the gut phageome of Japanese patients with ulcerative colitis under endoscopic remission.","authors":"Akinori Otsuki, Ryo Inoue, Takayuki Imai, Hiroto Miura, Atsushi Nishida, Osamu Inatomi, Akira Andoh","doi":"10.3164/jcbn.24-173","DOIUrl":"10.3164/jcbn.24-173","url":null,"abstract":"<p><p>This study aimed to analyze the gut phageome in Japanese patients with ulcerative colitis (UC) in endoscopic remission. Fecal samples were collected from 35 UC patients and 22 healthy controls. The gut microbiome was analyzed using 16S rRNA amplicon sequencing, and the phageome was profiled through shotgun metagenomic sequencing. Compared to healthy controls, UC patients showed a significant reduction in phageome richness (observed species and Chao1 index). Principal coordinate analysis revealed a significant difference in beta-diversity between UC and healthy controls (<i>p</i> = 0.001). The abundance of temperate phages was higher in UC (15.2%) compared to healthy controls (5.9%), although this was not statistically significant (<i>p</i> = 0.088). Temperate phages associated with <i>Coprococcus</i> sp., <i>Bacteroides</i> sp. KFT8, and <i>Faecalibacterium prausnitzii</i>, as well as virulent phages associated with <i>Ruminococcus gnavus</i> and <i>Lactobacillus farciminis</i>, were increased in UC patients. Conversely, phages associated with <i>Thermosipho affectus</i>, <i>Bacteroides</i> sp. OF03-11BH, and <i>Odoribacter splanchnicus</i> were decreased in UC patients. Phages associated with the genera <i>Odoribacter</i> (<i>p</i> = 0.0004), <i>Ruminococcus</i> (<i>p</i> = 0.009), and <i>Veillonella</i> (<i>p</i> = 0.013) were significantly reduced in UC patients. The gut phageome of inactive UC patients exhibited notable alterations in viral composition compared to healthy controls. These results suggest that changes in the gut phageome might be involved in the pathogenesis of UC.</p>","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"76 2","pages":"202-209"},"PeriodicalIF":2.0,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11936741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}