Journal of Clinical Biochemistry and Nutrition最新文献_第5页

Mechanism of lipid peroxidation of liposomes by cold atmospheric pressure plasma jet irradiation. 冷大气压等离子体射流辐照脂质体的脂质过氧化机理。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2024-11-01 Epub Date: 2024-08-09 DOI: 10.3164/jcbn.24-72

Tokuko Takajo, Koichi Saito, Kazunori Tsuchida, Shunji Kato, Kiyotaka Nakagawa, Akitoshi Okino, Kazunori Anzai

{"title":"Mechanism of lipid peroxidation of liposomes by cold atmospheric pressure plasma jet irradiation.","authors":"Tokuko Takajo, Koichi Saito, Kazunori Tsuchida, Shunji Kato, Kiyotaka Nakagawa, Akitoshi Okino, Kazunori Anzai","doi":"10.3164/jcbn.24-72","DOIUrl":"10.3164/jcbn.24-72","url":null,"abstract":"Liposome lipid peroxidation induced by cold atmospheric pressure plasma jet (CAPPJ) irradiation was investigated. The formation of thiobarbituric acid reactive substances (TBARS), an indicator of lipid peroxidation final products, as a function of irradiation was observed. Lipid radicals, peroxidation reaction intermediates generated by CAPPJ irradiation, were confirmed by increased NBD-pen fluorescence intensity. Additionally, lipid peroxidation products, liposomal phosphatidylcholine (PC) isomers, were analyzed by LC-MS/MS. Products specific to singlet oxygen (1O2) oxidation, 16:0/10-hydroperoxy-8E,12Z-octadecanoic acid (10-8E,12Z-HpODE) PC and 16:0/12-9E,13E-HpODE PC, were not detected, but radical oxidation specific products 16:0/13-9E,11E-HpODE PC and 16:0/9-10E,12E-HpODE PC were. This suggests that during CAPPJ irradiation, radicals, rather than 1O2, are the primary reactive species of lipid peroxidation. This is also supported by the β-carotene quenching of 1O2 not suppressing TBARS and lipid radical generation. Also, neither TBARS formation nor lipid radical generation were suppressed by SOD, indicating that the superoxide radical (O2 •-) is not responsible for the lipid peroxidation reaction. As the CAPPJ irradiation of water produces large quantities of hydroxyl radical (•OH) and •OH scavengers decreased the amount of TBARS produced by CAPPJ irradiation, it is highly plausible that •OH is the primary species involved in CAPPJ-induced liposome lipid peroxidation.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"75 3","pages":"183-189"},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579849/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterized factors of subjects who were first time diagnosed as hyperglycemia more than 126 mg/dl during annual or biannual medical checkups: a case-control study in Japan. 日本一项病例对照研究：年度或半年度体检中首次被诊断为高血糖超过 126 毫克/分升的受试者的特征因素。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2024-11-01 Epub Date: 2024-08-20 DOI: 10.3164/jcbn.24-98

Miwako Minami, Tomohiro Imamura, Ayako Takamori, Hiromu Minagawa, Rina Matsuo, Junko Miyakawa, Kenichi Izumi, Shoichiro Ohta, Kohei Yamanouchi, Nobuya Souta, Kyosuke Yamamoto, Sadatosi Tsuji, Tsukuru Umemura, Keizo Anzai, Yuji Hirai, Kazuma Fujimoto, Takuya Kishi

{"title":"Characterized factors of subjects who were first time diagnosed as hyperglycemia more than 126 mg/dl during annual or biannual medical checkups: a case-control study in Japan.","authors":"Miwako Minami, Tomohiro Imamura, Ayako Takamori, Hiromu Minagawa, Rina Matsuo, Junko Miyakawa, Kenichi Izumi, Shoichiro Ohta, Kohei Yamanouchi, Nobuya Souta, Kyosuke Yamamoto, Sadatosi Tsuji, Tsukuru Umemura, Keizo Anzai, Yuji Hirai, Kazuma Fujimoto, Takuya Kishi","doi":"10.3164/jcbn.24-98","DOIUrl":"10.3164/jcbn.24-98","url":null,"abstract":"The present study examined factors in subjects diagnosed with hyperglycemia during periodic medical checkups. In total, 9,324 subjects (males: 4,532, females: 4,792) visited the Takagi Hospital for medical checkups in 2019. Eighty-two subjects (59 males) whose fasting blood glucose exceeded 126 mg/dl for the first time during the annual or biannual follow-up were included. Sex- and age-matched controls were used. Data from cases with hyperglycemia were compared to data from themselves one or two years before hyperglycemia. Body mass index (BMI), waist circumference, fatty liver, and blood pressure were higher in cases than in controls. Fasting blood glucose and hemoglobin A1c were higher in cases. Blood test results indicated that triglyceride, low-density lipoprotein (LDL) cholesterol, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and γ-glutamyl transpeptidase (γ-GTP), were significantly enhanced in cases. Multiple logistic regression analysis revealed that BMI, waist circumference, blood pressure, triglyceride, ALT, and γ-GTP were significant independent risk factors for cases with hyperglycemia. These risk factors were already enhanced in the cases of themselves in one or two years before hyperglycemia. In conclusion, BMI, waist circumference, blood pressure, and fatty liver indicated by ALT and γ-GTP were exacerbated concomitant with hyperglycemia, and increases in these factors preceded hyperglycemia.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"75 3","pages":"217-221"},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of cerium oxide on iron metabolism in mice. 氧化铈对小鼠铁代谢的影响

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2024-11-01 Epub Date: 2024-08-27 DOI: 10.3164/jcbn.24-38

Yuki Kanome, Shunki Ohtomo, Masaharu Nakatsu, Masahiro Kohno, Koji Fukui

{"title":"Effect of cerium oxide on iron metabolism in mice.","authors":"Yuki Kanome, Shunki Ohtomo, Masaharu Nakatsu, Masahiro Kohno, Koji Fukui","doi":"10.3164/jcbn.24-38","DOIUrl":"10.3164/jcbn.24-38","url":null,"abstract":"The use of metal nanoparticles such as cerium oxide nanoparticles (nanoceria) in living organisms is attracting increasing attention. We administered nanoceria to chronic kidney disease model rats, including a 5/6 nephrectomy model and adenine administration model rats, and reported high phosphorus adsorption capacity and renal function improvement effects of nanoceria. However, the iron ion concentration in the serum fluctuated significantly after administration. Therefore, we investigated changes in proteins related to iron metabolism following administration of nanoceria to normal mice without chronic kidney disease over different periods of time. Nanoceria were administered to 10-week-old C57BL/6 mice for 4 or 12 weeks. Another group was administrated lanthanum carbonate, which is currently used as a phosphorus adsorbent. The amount of iron in the serum and the concentration of transferrin in the liver were significantly increased following nanoceria administration, and the amount of iron in the liver was significantly decreased. There were no changes in serum hepcidin, ferroportin, cholesterol, or low-density lipoprotein levels. These results indicate that nanoceria administration can affect iron metabolism in mice. Although the detailed mechanism remains unknown, caution is warranted when considering biological utilization in the future.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"75 3","pages":"190-196"},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative novel candy made from a low-solubility amorphous material promotes saliva secretion: a randomized, double-blind, placebo-controlled crossover comparative trial. 由低溶解度无定形材料制成的创新糖果可促进唾液分泌：随机、双盲、安慰剂对照交叉比较试验。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2024-11-01 Epub Date: 2024-08-09 DOI: 10.3164/jcbn.24-103

Shinpei Kawakami, Sadao Mori, Shota Kawasaki, Eisaku Nishimura, Mariko Yoshikawa, Akane Yamaguchi, Yuko Matsui, Toshihiro Kawama, Ichiro Saito

{"title":"Innovative novel candy made from a low-solubility amorphous material promotes saliva secretion: a randomized, double-blind, placebo-controlled crossover comparative trial.","authors":"Shinpei Kawakami, Sadao Mori, Shota Kawasaki, Eisaku Nishimura, Mariko Yoshikawa, Akane Yamaguchi, Yuko Matsui, Toshihiro Kawama, Ichiro Saito","doi":"10.3164/jcbn.24-103","DOIUrl":"10.3164/jcbn.24-103","url":null,"abstract":"Saliva has antioxidant properties, washes away food residues, and helps maintain the oral environment; thus, decreased saliva secretion can have negative consequences. This study examined how slow-soluble innovative candy, named low-solubility amorphous material, affects oral indices such as saliva secretion and halitosis in a randomized, double-blind, placebo-controlled, crossover comparative study. Twenty-four healthy individuals with low saliva production were given one piece of low-solubility amorphous material or placebo candy and their saliva secretion was measured over 20 min. Before and after participants used the test food, we measured the concentrations of three volatile sulfur compounds involved in halitosis and the secretion rate of secretory immunoglobulin A, and participants completed the Profile of Mood States Second Edition (POMS2) and a visual analog scale (VAS). As a result, saliva secretion increased significantly in low-solubility amorphous material candy condition, compared to placebo candy. Furthermore, changes in the hydrogen sulfide concentration, POMS2 Total Mood Disturbance and Vigor-Activity scores, and oral \"moisture\" and \"refreshed feeling\" scores on the VAS were improved more by low-solubility amorphous material candy use than by placebo. Low-solubility amorphous material candy may help improve the oral environment by increasing saliva secretion and reducing halitosis-related substances and may improve mood.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"75 3","pages":"222-227"},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

General and sex-specific effects of vitamin D against atrial fibrillation and young-onset ischemic stroke: a Mendelian randomization series. 维生素 D 对心房颤动和年轻缺血性中风的一般影响和性别特异性影响：孟德尔随机系列研究。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2024-11-01 Epub Date: 2024-07-12 DOI: 10.3164/jcbn.24-79

Yap-Hang Chan, Jie V Zhao, C Mary Schooling, Shiu-Lun Au Yeung, Yuen-Kwun Wong, Ka-Wing Au, Clara S Tang, Chloe Yu Yan Cheung, Aimin Xu, Pak-Chung Sham, Tai-Hing Lam, Karen Siu-Ling Lam, Hung-Fat Tse

{"title":"General and sex-specific effects of vitamin D against atrial fibrillation and young-onset ischemic stroke: a Mendelian randomization series.","authors":"Yap-Hang Chan, Jie V Zhao, C Mary Schooling, Shiu-Lun Au Yeung, Yuen-Kwun Wong, Ka-Wing Au, Clara S Tang, Chloe Yu Yan Cheung, Aimin Xu, Pak-Chung Sham, Tai-Hing Lam, Karen Siu-Ling Lam, Hung-Fat Tse","doi":"10.3164/jcbn.24-79","DOIUrl":"10.3164/jcbn.24-79","url":null,"abstract":"Whether vitamin D deficiency causes atrial fibrillation and ischemic stroke of young onset was unknown. We derived a Genetic Risk Score for vitamin D from 3,922 subjects in Hong Kong and applied it in an independent sample (n = 1,297) for clinical outcomes. Primary endpoint was a composite of atrial fibrillation and/or ischemic stroke. A second study was performed in the UK Biobank (n = 392,010; 46% men; 14,878 atrial fibrillation and 4,050 ischemic stroke cases, vs 374,102 controls). After 76 ± 46 months, 240 primary endpoints (18.5%) were adjudicated. Higher genetically-predicted vitamin D independently predicted reduced primary endpoint [odds ratio = 0.83 (0.72 to 0.95), p = 0.008]. Mendelian randomization analyses indicated vitamin D was causally protective against the primary endpoint [odds ratio = 0.81 (95% CI: 0.65 to 0.98)]. Independent analyses in the UK Biobank revealed that vitamin D was protective against young-onset ischemic stroke <50 years and atrial fibrillation combined [odds ratio = 0.36 (95% CI 0.14 to 0.94)], with predominant effect amongst men [odds ratio = 0.28 (95% CI 0.09 to 0.91)] compared to women [odds ratio = 0.60 (95% CI: 0.11 to 3.22)]. In conclusion, vitamin D may protect against young-onset ischemic stroke through preventing atrial fibrillation. Investigating the sex-specifc effects of vitamin D deficiency may elucidate sex disparities of atrial fibrillation in the young.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"75 3","pages":"228-236"},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Female patients with end-stage renal failure treated by hemodialysis had a low mortality rate and small patient number compared to male patients: 5-year follow-up study in Japan. 与男性患者相比，接受血液透析治疗的终末期肾衰竭女性患者死亡率低、人数少：日本 5 年随访研究。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2024-11-01 Epub Date: 2024-09-06 DOI: 10.3164/jcbn.24-141

Kuniyasu Takagi, Takuya Kishi, Taku Goto, Kohei Yamanouchi, Kazuhiko Yoshikawa, Tomohiro Imamura, Shiki Nakayama, Keizo Anzai, Yuichiro Akiyoshi, Akira Kitajima, Koji Onozawa, Ayako Takamori, Kazuma Fujimoto

{"title":"Female patients with end-stage renal failure treated by hemodialysis had a low mortality rate and small patient number compared to male patients: 5-year follow-up study in Japan.","authors":"Kuniyasu Takagi, Takuya Kishi, Taku Goto, Kohei Yamanouchi, Kazuhiko Yoshikawa, Tomohiro Imamura, Shiki Nakayama, Keizo Anzai, Yuichiro Akiyoshi, Akira Kitajima, Koji Onozawa, Ayako Takamori, Kazuma Fujimoto","doi":"10.3164/jcbn.24-141","DOIUrl":"10.3164/jcbn.24-141","url":null,"abstract":"This study aimed to evaluate gender differences of hemodialysis patients in adverse events, gastrointestinal bleeding, and bone fractures during 5 year longitudinal follow-up period in the regional core hospital in Japan. This study included 151 patients with maintenance hemodialysis for end-stage renal failure at Takagi Hospital in December 2017. All the patients, divided into females-group of 61 and males-group of 90. Data were evaluated in the electronic medical record. Multivariate analysis indicated a decrease in diabetes mellitus (odd ratio: 2.3, 95% confidence interval: 1.1-4.8, p = 0.03) and less mortality in those younger than 75 years old (odd ratio: 0.2, 95% confidence interval: 0.1-0.8, p = 0.02) were characterized factors in females. Gastrointestinal bleeding were not different between genders. Bone fractures were high in females (females: 34.4% vs males: 18.9%; p<0.03), whereas the mortality rate of bone fractured patients was markedly high in males (females: 28.6% vs males: 76.5%; p = 0.003) with lower body bone fractures. In conclusion, diabetes mellitus-induced end-stage renal failure was less common in females. The mortality rate during hemodialysis was higher in males less than 75 years old with increased mortality with lower bone fractures.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"75 3","pages":"237-240"},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of gut microbiota in inflammatory bowel disease pathogenesis. 肠道微生物群在炎症性肠病发病机制中的作用。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2024-11-01 Epub Date: 2024-09-06 DOI: 10.3164/jcbn.24-112

Yuji Naito, Tomohisa Takagi

引用次数: 0

Effect of omega-3 fatty acids on sleep: a systematic review and meta-analysis of randomized controlled trials. 欧米伽-3 脂肪酸对睡眠的影响：随机对照试验的系统回顾和荟萃分析。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2024-11-01 Epub Date: 2024-08-09 DOI: 10.3164/jcbn.24-36

Kaori Shimizu, Yui Kuramochi, Kohsuke Hayamizu

{"title":"Effect of omega-3 fatty acids on sleep: a systematic review and meta-analysis of randomized controlled trials.","authors":"Kaori Shimizu, Yui Kuramochi, Kohsuke Hayamizu","doi":"10.3164/jcbn.24-36","DOIUrl":"10.3164/jcbn.24-36","url":null,"abstract":"Omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs) have been reported to improve sleep quality in several studies, but meta-analyses have been inconclusive. We conducted this study to investigate the effects of omega-3 LC-PUFAs on sleep in clinical trials. The study was planned in accordance with the criteria of the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols (PRISMA-2020), and was performed by searching PubMed, The Cochrane Library, and Ichushi-web databases. Randomized controlled trials and clinical trials with control groups were included. Finally, eight studies were selected for inclusion in this study. Sleep efficiency was significantly higher in the omega-3 LC-PUFA group than in the control group, while sleep latency and total sleep duration did not differ significantly. Subjectively assessed sleep was significantly improved by omega-3 LC-PUFA, but heterogeneity was so high that a subgroup analysis based on dose of omega-3 supplementation was performed. It showed low heterogeneity and significant improvement in the omega-3 LC-PUFA group compared with the control group. Omega-3 LC-PUFAs have been shown to may improve sleep quality. Further studies are needed to confirm the relationship between omega-3 LC-PUFAs and sleep. The protocol for this review was registered in UMIN000052527.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"75 3","pages":"204-212"},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579846/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effective disruption of cancer cell membranes by photodynamic therapy with cell membrane-adhesive photosensitizer. 利用细胞膜附着光敏剂进行光动力疗法，有效破坏癌细胞膜。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2024-11-01 Epub Date: 2024-08-24 DOI: 10.3164/jcbn.24-88

Aoi Hoshi, Toru Yoshitomi, Yoshiki Komatsu, Naoki Kawazoe, Guoping Chen, Hiroko Bando, Hisato Hara, Hirofumi Matsui

{"title":"Effective disruption of cancer cell membranes by photodynamic therapy with cell membrane-adhesive photosensitizer.","authors":"Aoi Hoshi, Toru Yoshitomi, Yoshiki Komatsu, Naoki Kawazoe, Guoping Chen, Hiroko Bando, Hisato Hara, Hirofumi Matsui","doi":"10.3164/jcbn.24-88","DOIUrl":"10.3164/jcbn.24-88","url":null,"abstract":"Photodynamic therapy (PDT) is a noninvasive cancer treatment modality that involves the administration of photosensitizers and light irradiation. Previously, we established a polycation-containing hematoporphyrin (aHP) formulation that demonstrated superior antitumor efficacy in vivo, than the original hematoporphyrin (HP). In this study, we investigated underlining mechanisms of the high antitumor effect of aHP using cell experiments. Time-lapse imaging of rat gastric cancerous cell line (RGK45) treated with aHP exhibited swelling, cell rupture, and subsequent scattering of small vesicles upon light irradiation, in contrast to the small changes in morphology of RGK45 treated with HP. Furthermore, aHP presented concentrated localization on the cell membranes to a greater extent than HP. Additionally, neither aHP nor HP induced morphological changes in rat gastric mucosa cell line (RGM1). Flow cytometry analysis demonstrated a higher fluorescence of wheat germ agglutinin-conjugated dye in RGK45 than in RGM1, suggesting differential glycan expression patterns. These findings collectively suggest that the cellular toxicity of aHP may be augmented in RGK45 cells owing to its heightened affinity toward negatively charged structures on cellular membranes and its preferential localization on them. The observed membrane rupture and release of extracellular vesicles may confer an abscopal effect, in addition to direct PDT effect, thereby positioning aHP as a promising next-generation photosensitizer.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"75 3","pages":"197-203"},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vicious cycle of vitamin B₁ insufficiency and heart failure in cardiology outpatients. 心脏科门诊患者维生素 B1 不足与心力衰竭的恶性循环。

IF 2 4区医学

Journal of Clinical Biochemistry and Nutrition Pub Date : 2024-11-01 Epub Date: 2024-10-11 DOI: 10.3164/jcbn.24-137

Misora Ao, Kensuke Takabayashi, Rika Tomita, Ryoko Fujita, Takashi Miyawaki, Kiyoshi Tanaka

{"title":"Vicious cycle of vitamin B1 insufficiency and heart failure in cardiology outpatients.","authors":"Misora Ao, Kensuke Takabayashi, Rika Tomita, Ryoko Fujita, Takashi Miyawaki, Kiyoshi Tanaka","doi":"10.3164/jcbn.24-137","DOIUrl":"10.3164/jcbn.24-137","url":null,"abstract":"Heart failure is a major manifestation of vitamin B1 deficiency; beriberi. We have previously reported that even vitamin B1 insufficiency, milder than deficiency, is a risk for heart failure in the institutionalized elderly. Then in this cross-sectional study, sixty-eight cardiology outpatients were evaluated for their whole blood vitamin B1 and plasma brain natriuretic peptide (BNP) concentrations, a sensitive marker of heart failure, as well as their dietary intake. Whole blood vitamin B1 concentration was significantly correlated with plasma BNP level in vitamin B1-deficient/insufficient patients (whole blood vitamin B1<28 ‍ng/ml) but not in sufficient patients. Whole blood vitamin B1 concentration was significantly lower in loop diuretics users than non-users. Multiple regression analysis has identified whole blood vitamin B1 concentration and eGFR as the significant contributors to log-transformed plasm BNP level, and loop diuretics use, serum albumin level, and eGFR as the contributors to whole blood vitamin B1 concentration. ROC analysis has shown the significant predictability of whole blood vitamin B1 for plasma BNP ≥100 pg/ml with the cut-off value of 23.5 ‍ng/ml. Vitamin B1 insufficiency is a risk of heart failure in the cardiology outpatients, and the therapeutic use of loop diuretics aggravates heart failure and possibly forms a vicious cycle.","PeriodicalId":15429,"journal":{"name":"Journal of Clinical Biochemistry and Nutrition","volume":"75 3","pages":"241-246"},"PeriodicalIF":2.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11579856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0