Journal of Biological Engineering最新文献_第5页

Engineering NKG2D ligand affinity transforms EGFR-targeted NK cell engagers into high-potency effectors against pancreatic cancer. 工程NKG2D配体亲和力将egfr靶向NK细胞接合物转化为对抗胰腺癌的高效效应器。

IF 6.5 3区生物学

Journal of Biological Engineering Pub Date : 2026-03-10 DOI: 10.1186/s13036-026-00651-y

Seul-Gi Lee, Myungjee Lee, Hye-Min Lee, Ga-Hyun Son, Sang-Rok Yoon, Byeong-Ho Chae, Dae-Seong Kim, Kyung-Mi Lee, Yong-Sung Kim

引用次数: 0

Hybrid metabolic engineering enables xylose-driven co-production of polyhydroxybutyrate and violacein in Escherichia coli. 杂交代谢工程使木糖驱动的多羟基丁酸盐和紫罗兰素在大肠杆菌中共同生产。

IF 6.5 3区生物学

Journal of Biological Engineering Pub Date : 2026-03-09 DOI: 10.1186/s13036-026-00653-w

Khoi Nhat Pham, Eun Yeol Lee

引用次数: 0

A novel device for in-situ on-demand hydrogen sulfide generation and delivery to increase tissue perfusion to chronic wounds. 一种新型的原位按需硫化氢生成和输送装置，以增加慢性伤口的组织灌注。

IF 6.5 3区生物学

Journal of Biological Engineering Pub Date : 2026-03-09 DOI: 10.1186/s13036-026-00654-9

Matthew Justus, Debra Friedrichsen, Bill Brooks, Grayson Silaski, Robert Beckius, Pierce Massie, Carolyn Pace, Deepali Kulkarni, Trevor Self, Mildred Mattox, Jeffrey Bray, Erin Risotto-Urbanowicz, Andrea Iturralde-Carillo, Jenna Marek, Brittany Coffman, Cristine Heaps, Heather Ferguson, Reza Shekarriz, Nancy Kanagy, Ross Clark

引用次数: 0

Adaptive laboratory evolution with ethionine identifies novel genetic determinants for enhanced protein and methionine accumulation in Saccharomyces cerevisiae. 自适应实验室进化与蛋氨酸鉴定新的遗传决定因素增强蛋白质和蛋氨酸积累在酿酒酵母。

IF 6.5 3区生物学

Journal of Biological Engineering Pub Date : 2026-03-07 DOI: 10.1186/s13036-026-00652-x

Tae Hoon Lee, Sang-Hun Do, Hyun-Jae Lee, Kun-Jae Lee, Jonghyeok Shin, Yong-Cheol Park, Sun-Ki Kim

{"title":"Adaptive laboratory evolution with ethionine identifies novel genetic determinants for enhanced protein and methionine accumulation in Saccharomyces cerevisiae.","authors":"Tae Hoon Lee, Sang-Hun Do, Hyun-Jae Lee, Kun-Jae Lee, Jonghyeok Shin, Yong-Cheol Park, Sun-Ki Kim","doi":"10.1186/s13036-026-00652-x","DOIUrl":"10.1186/s13036-026-00652-x","url":null,"abstract":"Enhancing methionine and protein content in Saccharomyces cerevisiae is essential for its use as single-cell protein. Here, we applied ethionine resistance-mediated adaptive laboratory evolution (ALE) to generate strains with improved resistance to this toxic methionine analog. Stepwise adaptation enabled growth at ethionine concentrations of up to 0.50 mM and yielded strains with progressively higher intracellular methionine levels and improved protein production efficiency. Whole-genome sequencing identified nonsynonymous SNPs in 32 genes, of which nine candidates were functionally validated. CRISPR/Cas9-based editing demonstrated that mutations in MDE1 and JJJ1 directly elevated free methionine levels, whereas most other mutations increased overall protein accumulation. Functional annotations linked these genes to RNA processing, protein degradation, methionine salvage, and amino acid uptake, highlighting RNA processing as a major target for global protein enhancement. These findings reveal that ethionine resistance-mediated ALE induces multifactorial adaptations. They also provide new insights into protein biosynthesis regulation and lay a foundation for future engineering of high-performance yeast strains.","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13081643/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147372628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bakuchiol-loaded amphiphilic polymeric nanoparticles: enhancing stability and multifunctionality through encapsulation. bakuchiol负载的两亲性聚合物纳米颗粒：通过封装增强稳定性和多功能性。

IF 6.5 3区生物学

Journal of Biological Engineering Pub Date : 2026-03-03 DOI: 10.1186/s13036-026-00644-x

Dongseong Seo, Sohyeon Yu, Taeho Kim, Jonghyun Park, Hyungjun Kim, Daekyung Sung

{"title":"Bakuchiol-loaded amphiphilic polymeric nanoparticles: enhancing stability and multifunctionality through encapsulation.","authors":"Dongseong Seo, Sohyeon Yu, Taeho Kim, Jonghyun Park, Hyungjun Kim, Daekyung Sung","doi":"10.1186/s13036-026-00644-x","DOIUrl":"10.1186/s13036-026-00644-x","url":null,"abstract":"Bakuchiol (BK), a natural meroterpene isolated from Psoralea corylifolia, has gained significant attention as a functional cosmetic ingredient due to its antioxidant, anti-inflammatory, and antibacterial properties. However, its high hydrophobicity and chemical instability limit its practical application. In this study, we developed a robust and efficient delivery system for BK using polyglyceryl-10 laurate (PG10-L), an amphiphilic polymer, via a simple and scalable nanoprecipitation strategy. This approach helps address an important challenge in achieving stable long-term aqueous formulations of BK without relying on complex synthetic processes. The resulting BK-loaded nanoparticles (BK@NPs) exhibited a small particle size (< 200 nm), high encapsulation efficiency (≈ 99%), and remarkable colloidal stability for over 12 weeks. Notably, BK@NPs significantly enhanced biological efficacy compared to free BK, resulting in markedly improved antioxidant and antibacterial performance. Furthermore, the formulation demonstrated excellent cytocompatibility in NIH-3T3 murine fibroblasts along with effective suppression of intracellular reactive oxygen species (ROS). These findings highlight that the PG10-L-based nanoprecipitation strategy provides a robust and green platform to enhance the multifunctional potential of bakuchiol for advanced dermo-cosmetic applications.","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13064261/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147344237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the impact of multinational collaborations on cultural understanding, health disparities, biomedical innovations, and professional development through project-based learning. 通过基于项目的学习，调查跨国合作对文化理解、健康差异、生物医学创新和专业发展的影响。

IF 6.5 3区生物学

Journal of Biological Engineering Pub Date : 2026-03-02 DOI: 10.1186/s13036-026-00646-9

Breanna Kilgore, Nickolas A Gustavison, Alexandra Guiterrez-Vega, Ernesto Ibarra-Ramírez, Luis Estrada-Petrocelli, Jay Jesus Molino, Shilpa Drakshi, Anil S Bilimale, Devanshi Somaiya, Abhishek Appaji, Rogelio Garcia Contreras, Raj R Rao

引用次数: 0

Engineered chitosan/HP-β-CD hydrogel for targeted co-delivery of curcumin and berberine for the treatment of ulcerative colitis. 工程壳聚糖/HP-β-CD水凝胶靶向共递送姜黄素和小檗碱治疗溃疡性结肠炎。

IF 6.5 3区生物学

Journal of Biological Engineering Pub Date : 2026-02-28 DOI: 10.1186/s13036-026-00621-4

Leilei Li, Meilan Wang, Shiping Zhou, Yuanting Yang, Sileng Hu, Hongyan Li, Yuqin Li

{"title":"Engineered chitosan/HP-β-CD hydrogel for targeted co-delivery of curcumin and berberine for the treatment of ulcerative colitis.","authors":"Leilei Li, Meilan Wang, Shiping Zhou, Yuanting Yang, Sileng Hu, Hongyan Li, Yuqin Li","doi":"10.1186/s13036-026-00621-4","DOIUrl":"10.1186/s13036-026-00621-4","url":null,"abstract":"Ulcerative colitis (UC) presents considerable challenges in clinical treatment due to its multifaceted and complex nature. Efficacious therapy technique in developing an oral formulation that can encapsulate and deliver therapeutic drugs directly to the colon to diminish intestinal inflammation and restore the impaired intestinal barrier is of great interest. Herein, we developed a supramolecular hydrogel platform composed of 2-hydroxypropyl-β-cyclodextrin/chitosan in which curcumin and berberine (HCD/CH-CUR-BBR) are loaded in the hydrophobic cavity of HP-β-CD. BET surface area was measured to be 198.3 m2g- 1 with a pore diameter of 3.56 μm which improved in vitro drug release. The results demonstrate that the HCD/CH-CUR-BBR hydrogel leverages pH sensitivity to achieve sustained drug release in the colon while promoting targeted delivery to colonic tissues. Interestingly, in vitro and in vivo evaluation confirms that HCD/CH-CUR-BBR hydrogel formulation alleviates the progression of UC via downregulating IL-6 and TNF-α expression. Subsequently, in vivo anti-colitis investigation demonstrated notable enhancement in tight junction protein expression, underscoring anti-inflammatory efficiency of CUR and BBR loaded HCD/CH hydrogel. These findings indicate that the HCD/CH hydrogel infused with CUR and BBR displayed potential as an effective targeted drug delivery strategy for UC treatment.","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13059576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147317125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of AS1411 aptamer-conjugated chitosan-carbon dot nanocarriers for targeted drug delivery and fluorescent imaging in breast cancer therapy. AS1411适配体偶联壳聚糖碳点纳米载体在乳腺癌治疗中的靶向药物传递和荧光成像研究

IF 6.5 3区生物学

Journal of Biological Engineering Pub Date : 2026-02-27 DOI: 10.1186/s13036-026-00643-y

Mohammad Jafar Mantashlou, Effat Alizadeh, Sevil Vaghefi Moghaddam, Neda Saraygord Afshari, Amir Noorbakhsh

引用次数: 0

Evolvoid: a genetic algorithm for shaping optimal cellular constructs. 进化：一种用于塑造最佳细胞结构的遗传算法。

IF 6.5 3区生物学

Journal of Biological Engineering Pub Date : 2026-02-27 DOI: 10.1186/s13036-026-00642-z

Piera Mancini, Flavio Fontana, Ermes Botte, Chiara Magliaro, Francesco Pampaloni, Arti Ahluwalia

{"title":"Evolvoid: a genetic algorithm for shaping optimal cellular constructs.","authors":"Piera Mancini, Flavio Fontana, Ermes Botte, Chiara Magliaro, Francesco Pampaloni, Arti Ahluwalia","doi":"10.1186/s13036-026-00642-z","DOIUrl":"10.1186/s13036-026-00642-z","url":null,"abstract":"Background: Accurate cellular models are critical for understanding tissue function and accelerating drug discovery. While in vitro systems like spheroids and organoids are widely used, they are costly, low-throughput, and often lack reproducibility. Here, we introduce Evolvoid, a computational pipeline based on genetic algorithms that generates virtual 3D spheroid-like cell constructs with optimized morphologies by simulating key biophysical principles - such as thermodynamic principles, nutrient transport and their uptake by cells.Results: By integrating finite element simulations with evolutionary principles, our in silico platform evolves populations of randomly generated shapes, iteratively selecting individuals that best satisfy a biophysically informed fitness function. The fitness function encodes selection pressures based on general biophysical constraints - namely, the minimization of surface energy and the maintenance of cell viability under variable oxygen conditions. The Shannon entropy is used to track genome complexity over generations. In consistence with evolutionary dynamics, the complexity increases progressively. Moreover, albeit based on basic biophysical optimization rules, the fittest individuals generated by Evolvoid closely resemble the shape and size of cellular spheroids obtained in vitro.Conclusion: Evolvoid is modular, scalable, and tunable to different construct sizes, cell types, or culture conditions, and thus provides a versatile platform for designing and optimizing 3D cellular models entirely in silico. It offers a foundation for developing high-fidelity, cost-effective digital shape twins of biological systems, supporting the advancement of lab-on-a-laptop technologies and new approach methodologies, thereby reducing the reliance on in vitro and animal models.","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13049953/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147317121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of PGCL/AP conductive composite scaffold combined with electrical stimulation in neural tissue engineering. PGCL/AP导电复合支架结合电刺激在神经组织工程中的应用。

IF 6.5 3区生物学

Journal of Biological Engineering Pub Date : 2026-02-25 DOI: 10.1186/s13036-026-00620-5

Longchuan Zhu, Hongye Yan, Hongru Li, Su Pan, Hanwen Yang, Zhe Zhu, Zhiping Qi

引用次数: 0