{"title":"含有氧化锌纳米粒子和无细胞脂肪提取物的核壳纳米纤维敷料：增强成纤维细胞活性和抗菌功效。","authors":"Aydin Mahmoudnezhad, Mahsa Bayrami, Mahdiyeh Saadati, Yavuz Nuri Ertaş, Mozhgan Abasi, Aylar Ebrahimi, Younes Pilehvar","doi":"10.1186/s13036-025-00511-1","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>This study presents the development and characterization of innovative core-shell nanofiber wound dressings incorporating zinc oxide nanoparticles (nZnO) and cell-free fat extract (CEFFE) to enhance fibroblast activity and antibacterial efficacy.</p><p><strong>Results: </strong>CEFFE was prepared and analyzed, revealing high concentrations of essential growth factors, particularly bFGF and TGF-β1, supporting its therapeutic potential in tissue regeneration. The fabricated nanofibers (PLCL, nZnO/PLCL, PLCL-CEFFE/HA, and nZnO/PLCL-CEFFE/HA) were examined using FE-SEM and TEM, demonstrating successful encapsulation and morphological variations due to nZnO incorporation. XRD analysis confirmed the structural integrity and effective loading of nZnO and CEFFE. Hydrophilicity assessment via water contact angle measurements showed that CEFFE/HA significantly enhanced the hydrophilicity of PLCL membranes, crucial for wound exudate management. Mechanical tests indicated that CEFFE/HA addition maintained the scaffold's mechanical robustness, while nZnO slightly reduced mechanical properties. In vitro release studies revealed a biphasic release pattern of Zn²⁺ ions and growth factors from nZnO/PLCL-CEFFE/HA nanofibers, ensuring prolonged antibacterial activity and sustained therapeutic effects. Antibacterial assays demonstrated significant efficacy against E. coli and S. aureus, attributed to nZnO. MTT assays and FE-SEM analysis confirmed enhanced NIH-3T3 cell proliferation and adhesion on PLCL-CEFFE/HA nanofibers due to the controlled release of growth factors. The scratch assay showed superior cell migration and wound healing potential for PLCL-CEFFE/HA formulations.</p><p><strong>Conclusions: </strong>These findings underscore the potential of nZnO/PLCL-CEFFE/HA core-shell nanofibers as multifunctional wound dressings, combining antibacterial properties with enhanced tissue regeneration capabilities. However, further studies are needed to assess long-term stability and in vivo performance, which represent key challenges for future research.</p>","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":"19 1","pages":"46"},"PeriodicalIF":5.7000,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12090510/pdf/","citationCount":"0","resultStr":"{\"title\":\"Core-shell nanofiber dressings with zinc oxide nanoparticles and cell-free fat extract: boosting fibroblast activity and antibacterial efficacy.\",\"authors\":\"Aydin Mahmoudnezhad, Mahsa Bayrami, Mahdiyeh Saadati, Yavuz Nuri Ertaş, Mozhgan Abasi, Aylar Ebrahimi, Younes Pilehvar\",\"doi\":\"10.1186/s13036-025-00511-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>This study presents the development and characterization of innovative core-shell nanofiber wound dressings incorporating zinc oxide nanoparticles (nZnO) and cell-free fat extract (CEFFE) to enhance fibroblast activity and antibacterial efficacy.</p><p><strong>Results: </strong>CEFFE was prepared and analyzed, revealing high concentrations of essential growth factors, particularly bFGF and TGF-β1, supporting its therapeutic potential in tissue regeneration. The fabricated nanofibers (PLCL, nZnO/PLCL, PLCL-CEFFE/HA, and nZnO/PLCL-CEFFE/HA) were examined using FE-SEM and TEM, demonstrating successful encapsulation and morphological variations due to nZnO incorporation. XRD analysis confirmed the structural integrity and effective loading of nZnO and CEFFE. Hydrophilicity assessment via water contact angle measurements showed that CEFFE/HA significantly enhanced the hydrophilicity of PLCL membranes, crucial for wound exudate management. Mechanical tests indicated that CEFFE/HA addition maintained the scaffold's mechanical robustness, while nZnO slightly reduced mechanical properties. In vitro release studies revealed a biphasic release pattern of Zn²⁺ ions and growth factors from nZnO/PLCL-CEFFE/HA nanofibers, ensuring prolonged antibacterial activity and sustained therapeutic effects. Antibacterial assays demonstrated significant efficacy against E. coli and S. aureus, attributed to nZnO. MTT assays and FE-SEM analysis confirmed enhanced NIH-3T3 cell proliferation and adhesion on PLCL-CEFFE/HA nanofibers due to the controlled release of growth factors. The scratch assay showed superior cell migration and wound healing potential for PLCL-CEFFE/HA formulations.</p><p><strong>Conclusions: </strong>These findings underscore the potential of nZnO/PLCL-CEFFE/HA core-shell nanofibers as multifunctional wound dressings, combining antibacterial properties with enhanced tissue regeneration capabilities. However, further studies are needed to assess long-term stability and in vivo performance, which represent key challenges for future research.</p>\",\"PeriodicalId\":15053,\"journal\":{\"name\":\"Journal of Biological Engineering\",\"volume\":\"19 1\",\"pages\":\"46\"},\"PeriodicalIF\":5.7000,\"publicationDate\":\"2025-05-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12090510/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Biological Engineering\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1186/s13036-025-00511-1\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Biological Engineering","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1186/s13036-025-00511-1","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Core-shell nanofiber dressings with zinc oxide nanoparticles and cell-free fat extract: boosting fibroblast activity and antibacterial efficacy.
Background: This study presents the development and characterization of innovative core-shell nanofiber wound dressings incorporating zinc oxide nanoparticles (nZnO) and cell-free fat extract (CEFFE) to enhance fibroblast activity and antibacterial efficacy.
Results: CEFFE was prepared and analyzed, revealing high concentrations of essential growth factors, particularly bFGF and TGF-β1, supporting its therapeutic potential in tissue regeneration. The fabricated nanofibers (PLCL, nZnO/PLCL, PLCL-CEFFE/HA, and nZnO/PLCL-CEFFE/HA) were examined using FE-SEM and TEM, demonstrating successful encapsulation and morphological variations due to nZnO incorporation. XRD analysis confirmed the structural integrity and effective loading of nZnO and CEFFE. Hydrophilicity assessment via water contact angle measurements showed that CEFFE/HA significantly enhanced the hydrophilicity of PLCL membranes, crucial for wound exudate management. Mechanical tests indicated that CEFFE/HA addition maintained the scaffold's mechanical robustness, while nZnO slightly reduced mechanical properties. In vitro release studies revealed a biphasic release pattern of Zn²⁺ ions and growth factors from nZnO/PLCL-CEFFE/HA nanofibers, ensuring prolonged antibacterial activity and sustained therapeutic effects. Antibacterial assays demonstrated significant efficacy against E. coli and S. aureus, attributed to nZnO. MTT assays and FE-SEM analysis confirmed enhanced NIH-3T3 cell proliferation and adhesion on PLCL-CEFFE/HA nanofibers due to the controlled release of growth factors. The scratch assay showed superior cell migration and wound healing potential for PLCL-CEFFE/HA formulations.
Conclusions: These findings underscore the potential of nZnO/PLCL-CEFFE/HA core-shell nanofibers as multifunctional wound dressings, combining antibacterial properties with enhanced tissue regeneration capabilities. However, further studies are needed to assess long-term stability and in vivo performance, which represent key challenges for future research.
期刊介绍：
Biological engineering is an emerging discipline that encompasses engineering theory and practice connected to and derived from the science of biology, just as mechanical engineering and electrical engineering are rooted in physics and chemical engineering in chemistry. Topical areas include, but are not limited to:
Synthetic biology and cellular design
Biomolecular, cellular and tissue engineering
Bioproduction and metabolic engineering
Biosensors
Ecological and environmental engineering
Biological engineering education and the biodesign process
As the official journal of the Institute of Biological Engineering, Journal of Biological Engineering provides a home for the continuum from biological information science, molecules and cells, product formation, wastes and remediation, and educational advances in curriculum content and pedagogy at the undergraduate and graduate-levels.
Manuscripts should explore commonalities with other fields of application by providing some discussion of the broader context of the work and how it connects to other areas within the field.
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