Journal of Biological Engineering最新文献

Design of a prodrug bispecific antibody masked by a functional molecule for lymphocyte activation for cancer therapy. 一种被功能性分子掩盖的前药双特异性抗体的设计，用于淋巴细胞活化的癌症治疗。

IF 5.7 3区生物学

Journal of Biological Engineering Pub Date : 2025-05-15 DOI: 10.1186/s13036-025-00517-9

Daimei Miura, Yuki Kato, Masahiro Yasunaga, Izumi Kumagai, Ryutaro Asano

{"title":"Design of a prodrug bispecific antibody masked by a functional molecule for lymphocyte activation for cancer therapy.","authors":"Daimei Miura, Yuki Kato, Masahiro Yasunaga, Izumi Kumagai, Ryutaro Asano","doi":"10.1186/s13036-025-00517-9","DOIUrl":"10.1186/s13036-025-00517-9","url":null,"abstract":"Although T cells engaging bispecific antibodies (T-bsAbs) have shown great benefits, their use in treating solid tumors is challenging because of the minimal infiltration of T-cells. We fused an agonistic single-chain variable fragment (scFv) that induces a T cell co-stimulatory signal to the T cell-binding domain of T-bsAb via a linker containing a cancer-specific protease recognition site. With this antibody format, unexpected cytotoxicity to the surrounding normal tissue would be reduced and tumor-specific T cell activation would occur. The scFv-masked T-bsAb was cleaved by collagenase with intrinsic cancer-specific protease activity, releasing agonistic scFv without unwanted fragmentation and restoring the binding ability of the scFv-masked bsAbs to T cells. Compared to the original bsAb, a detectable enhancement of the T cell proliferation and cancer cytotoxicity was observed after the incubation with collagenase or protease-secretory cancer cells, which was suggested to be due to the modest co-stimulation by the released agonistic scFv. Our results provide important insights into an ideal T-bsAb prodrug format, precisely engineered to reduce side effects and exert high cancer cytotoxicity for solid tumor precision medicine.","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":"19 1","pages":"45"},"PeriodicalIF":5.7,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12079947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From microbial proteins to cultivated meat for alternative meat-like products: a review on sustainable fermentation approaches. 从微生物蛋白到人造肉的替代类肉产品：可持续发酵方法综述。

IF 5.7 3区生物学

Journal of Biological Engineering Pub Date : 2025-05-14 DOI: 10.1186/s13036-025-00509-9

Fernando Roberto Paz Cedeno, Olumide Joseph Olubiyo, Sungil Ferreira

{"title":"From microbial proteins to cultivated meat for alternative meat-like products: a review on sustainable fermentation approaches.","authors":"Fernando Roberto Paz Cedeno, Olumide Joseph Olubiyo, Sungil Ferreira","doi":"10.1186/s13036-025-00509-9","DOIUrl":"https://doi.org/10.1186/s13036-025-00509-9","url":null,"abstract":"The global demand for protein is rapidly increasing due to population growth and changing dietary preferences, highlighting the need for sustainable alternatives to traditional animal-based proteins. This review explores cultivated meat and microbial alternative proteins, focusing on their potential to meet nutritional needs while mitigating environmental impacts. It also examines the production of cultivated meat as well as various sources of microbial proteins, including mycoproteins, bacterial proteins, and microalgae, highlighting their nutritional profiles, production methods, and commercial applications. This includes an evaluation of the state of commercialization of mycoproteins and the innovative use of agricultural and industrial by-products as substrates for microbial fermentation. The integration of microbial protein production with the bioenergy sector is evaluated as a relevant alternative to attain a synergetic effect between energy and food production systems. Ultimately, this work aims to underscore the importance of microbial proteins in advancing towards a more sustainable protein production system, offering insights into current challenges and future opportunities in the field of fermentation to produce alternative proteins.","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":"19 1","pages":"44"},"PeriodicalIF":5.7,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12077041/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144077901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of antifoam agents on Spodoptera frugiperda 9 cell growth and baculovirus infection dynamics. 消泡剂对夜蛾细胞生长及杆状病毒感染动态的影响。

IF 5.7 3区生物学

Journal of Biological Engineering Pub Date : 2025-05-10 DOI: 10.1186/s13036-025-00516-w

Kristina Worch, Merlin Krause, Antje Burse

{"title":"Effects of antifoam agents on Spodoptera frugiperda 9 cell growth and baculovirus infection dynamics.","authors":"Kristina Worch, Merlin Krause, Antje Burse","doi":"10.1186/s13036-025-00516-w","DOIUrl":"https://doi.org/10.1186/s13036-025-00516-w","url":null,"abstract":"The baculovirus expression system is widely used for recombinant protein production. However, its scalability under shaking or stirring cultivation conditions remains a challenge due to foam formation which can negatively affect cell metabolism and viability, complicate process control, and ultimately lower productivity. Compared to other protein expression platforms, the effect of antifoam agents on insect cell culture has been rarely investigated. This study examines the influence of three antifoam agents-Antifoam 204 (AF204), polypropylene glycol (PPG), and a silicone-based compound (SAG471)-on Spodoptera frugiperda (Sf) 9 cell growth, viability, baculovirus infectivity, and infection dynamics. Dose-response experiments in adherent culture showed that high antifoam concentrations inhibited metabolic activity. In suspension culture, low concentrations of AF204 and PPG enhanced cell growth by reducing lag phase and population doubling time, while growth with SAG471 remained comparable to a no-antifoam control. In virus titer experiments, no effects on the plaque-forming ability of baculovirus particles could be observed. However, infection dynamics monitored in suspension cultures improved in the presence of all three antifoam agents, as shown by cell size increase, living cell stagnation, and enhanced single-cell fluorescence. Foam reduction experiments demonstrated that only SAG471 contributed to foam removal within a non-toxic concentration range. The results indicate that antifoam agents, depending on their concentration and composition, can enhance Sf9 cell growth and viability while potentially modulating cell membrane properties that could improve viral infection efficiency and transfection efficiency of exogenous material. This highlights the potential of antifoam agents for optimizing other virus-based expression systems in higher eukaryotic cells.","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":"19 1","pages":"43"},"PeriodicalIF":5.7,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12065184/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenolic-loaded nanofiber from Arctium lappa root: a potential therapy for testosterone-induced ovarian oxidative stress. 牛蒡根的酚负载纳米纤维：睾丸激素诱导的卵巢氧化应激的潜在疗法。

IF 5.7 3区生物学

Journal of Biological Engineering Pub Date : 2025-05-09 DOI: 10.1186/s13036-025-00515-x

Viana Khojasteh, Seyed Amirmohammad Mollanorouzi, Ehsan Karimi, Behrouz Ghorani, Ehsan Oskoueian

{"title":"Phenolic-loaded nanofiber from Arctium lappa root: a potential therapy for testosterone-induced ovarian oxidative stress.","authors":"Viana Khojasteh, Seyed Amirmohammad Mollanorouzi, Ehsan Karimi, Behrouz Ghorani, Ehsan Oskoueian","doi":"10.1186/s13036-025-00515-x","DOIUrl":"https://doi.org/10.1186/s13036-025-00515-x","url":null,"abstract":"Polycystic Ovary Syndrome (PCOS) is a hormonal disorder affecting women of reproductive age, often associated with oxidative stress and inflammation. This study explores the therapeutic potential of Arctium lappa phenolic-rich fraction encapsulated nanofiber (ALPRF-NF) in a testosterone-induced PCOS mouse model. All experiments were performed in triplicate and Duncan's Multiple Range Test was used to assess significant differences between means, with significance determined at p < 0.05. The ALPRF-NF formulation demonstrated favorable physicochemical properties, including a ribbon-like structure (216.9 nm), a zeta potential of -19.3 mV, and a high encapsulation efficiency (93.1%). In vivo findings showed that ALPRF-NF significantly improved body weight, feed intake, and liver enzyme profiles in PCOS-induced mice (p ≤ 0.05). It also enhanced the antioxidant defense system by elevating levels of glutathione peroxidase (GPX), superoxide dismutase (SOD), and catalase (CAT). Mechanistically, ALPRF-NF reduced oxidative stress and inflammation by delivering phenolic compounds that scavenge reactive oxygen species (ROS) and modulate gene expression in ovarian tissue. This included downregulation of inducible nitric oxide synthase (iNOS) and upregulation of SOD expression. These results suggest that ALPRF-NF effectively mitigates testosterone-induced ovarian oxidative damage and inflammation, offering a targeted, nanotechnology-based therapeutic approach for PCOS. The study provides valuable insights into novel strategies for improving women's reproductive health through bioactive compound delivery.","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":"19 1","pages":"42"},"PeriodicalIF":5.7,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12065167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143991402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-throughput strategies for monoclonal antibody screening: advances and challenges. 单克隆抗体筛选的高通量策略：进展和挑战。

IF 5.7 3区生物学

Journal of Biological Engineering Pub Date : 2025-05-08 DOI: 10.1186/s13036-025-00513-z

Xiao-Dong Wang, Bao-Ying Ma, Shi-Ying Lai, Xiang-Jing Cai, Yan-Guang Cong, Jun-Fa Xu, Peng-Fei Zhang

引用次数: 0

Engineered supercooling systems for enhanced long-term preservation of large-volume red blood cells in commercial blood bags. 工程过冷系统，提高长期保存大容量红细胞在商业血袋。

IF 5.7 3区生物学

Journal of Biological Engineering Pub Date : 2025-05-06 DOI: 10.1186/s13036-025-00510-2

Qi Liu, Shichun Wang, Jie Yan, Ronghua Diao, Haishui Huang, Feng Xu, Chunyan Yao

{"title":"Engineered supercooling systems for enhanced long-term preservation of large-volume red blood cells in commercial blood bags.","authors":"Qi Liu, Shichun Wang, Jie Yan, Ronghua Diao, Haishui Huang, Feng Xu, Chunyan Yao","doi":"10.1186/s13036-025-00510-2","DOIUrl":"https://doi.org/10.1186/s13036-025-00510-2","url":null,"abstract":"Reducing cell metabolism by lowering the storage temperature is an important method to improve the quality of stored RBCs and prolong the stored shelf life of RBCs. Traditional cryopreservation suffers from limitations such as tedious cytotoxic cryoprotectants (CPA) loading, unloading and ice-induced damage. Storage around 2-6 °C is an alternative method but only works for a short period due to significant storage lesions at this high storage temperature. We developed an improved supercooling preservation system for large-volume (100 ml) RBC suspensions in commercial polyvinylchloride (PVC) blood bags by minimizing favorable sites of ice nucleation and maintaining precise thermal control at -8 °C. This engineered protocol significantly reduces hemolysis, metabolic degradation, and oxidative stress while preserving RBC membrane integrity and functionality for up to 63 days. In vivo transfusion studies in New Zealand white rabbits demonstrate that supercooling-preserved RBCs achieve higher post-transfusion recovery rates, outperforming conventional storage methods. Our scalable and cost-effective supercooling system address critical needs for improving the quality of stored RBCs by achieving ice-free preservation, which representing a significant breakthrough in transfusion medicine.","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":"19 1","pages":"40"},"PeriodicalIF":5.7,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12054195/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cerium-doped calcium carbonate microparticles combined with low-intensity ultrasound for efficient sonodynamic therapy in body sculpting. 掺铈碳酸钙微颗粒结合低强度超声在人体塑形中的高效声动力治疗。

IF 5.7 3区生物学

Journal of Biological Engineering Pub Date : 2025-04-28 DOI: 10.1186/s13036-025-00505-z

Jhih-Ni Lin, Chih-Ying Chi, Yu-Ying Lin, Che-Yung Kuan, Chia-Tien Chang, Li-Ze Lin, I-Hsuan Yang, Feng-Huei Lin

{"title":"Cerium-doped calcium carbonate microparticles combined with low-intensity ultrasound for efficient sonodynamic therapy in body sculpting.","authors":"Jhih-Ni Lin, Chih-Ying Chi, Yu-Ying Lin, Che-Yung Kuan, Chia-Tien Chang, Li-Ze Lin, I-Hsuan Yang, Feng-Huei Lin","doi":"10.1186/s13036-025-00505-z","DOIUrl":"https://doi.org/10.1186/s13036-025-00505-z","url":null,"abstract":"Excessive caloric intake and reduced energy expenditure contribute to obesity and localized fat accumulation, adversely affecting overall health. Despite advancements in obesity treatments, noninvasive approaches for targeted fat reduction remain limited. This study introduces a novel sonosensitizer microparticle, cerium-doped calcium carbonate (CaCO3:Ce), and evaluates its potential application in combination with low-intensity ultrasound (LIUS) for noninvasive body sculpting. CaCO3:Ce was synthesized via an environmentally friendly method, producing uniform 1.77 μm particles optimized for endocytosis. Energy-dispersive X-ray and X-ray photoelectron spectroscopy confirmed successful cerium doping. The particles demonstrated excellent biocompatibility and generated reactive oxygen species under LIUS exposure. Safety was validated through biochemical, hematological, and histological analyses in Sprague-Dawley rats. Animal experiments further revealed that CaCO3:Ce combined with ultrasound significantly reduced body weight growth rates, waistline measurements, and subcutaneous fat accumulation. These findings suggest that CaCO3:Ce, coupled with LIUS, offers a promising, noninvasive, and low-risk strategy for body sculpting, addressing limitations of current methodologies.","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":"19 1","pages":"39"},"PeriodicalIF":5.7,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12036128/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetically engineered secretory horseradish peroxidase is a sensitive, stable, and affordable non-lytic reporter gene system for real-time promoter activity management. 基因工程分泌型辣根过氧化物酶是一种敏感、稳定、价格合理的非裂解型报告基因系统，用于实时启动子活性管理。

IF 5.7 3区生物学

Journal of Biological Engineering Pub Date : 2025-04-22 DOI: 10.1186/s13036-025-00508-w

Mu-Shen Chang, Chia-Yi Lee, Yu-Yen Chang, Pin-Jie Li, Hsin-Yu Wu, En-Shuo Liu, Hsin-Kai Huang, Wen-Wei Lin

{"title":"Genetically engineered secretory horseradish peroxidase is a sensitive, stable, and affordable non-lytic reporter gene system for real-time promoter activity management.","authors":"Mu-Shen Chang, Chia-Yi Lee, Yu-Yen Chang, Pin-Jie Li, Hsin-Yu Wu, En-Shuo Liu, Hsin-Kai Huang, Wen-Wei Lin","doi":"10.1186/s13036-025-00508-w","DOIUrl":"https://doi.org/10.1186/s13036-025-00508-w","url":null,"abstract":"A light-producing secretory protein that is collectible through the supernatant of a culture medium is essential in a cell-based reporter gene system and allows for real-time monitoring of upstream events of a promoter. Compared to other secretory luciferases, Cypridina luciferase (CLuc) coupled with vargulin emits the brightest signal; however, the signal half-life suffers constantly from the fast oxidation process of the substrate, resulting in a rapid signal depletion, which makes the detection signal short and unstable. In this study, we aimed to develop a new reporter gene system with a more stable signal and lower cost, whilst retaining sensitivity comparable to the CLuc reporter gene system. To this end, we genetically engineered horseradish peroxidase (HRP) to be secreted with mammalian cells. The secreted form HRP (sHRP) was then used as a proof-of-concept of real-time cell signaling management. First, we made sure that HRP retained its enzymatic function with our genetic engineering process and confirmed that it was collectable and suitable for side-by-side comparison with CLuc. sHRP showed comparable sensitivity, 7 to 80 times more signal half-life compared to CLuc, and precisely reported NF-κB-regulated promoter in response to stimulation with TNF-α. sHRP was not affected by multiple cell culturing media and was calculated to be at least 9 times cheaper than the CLuc reporter gene system. Thus, sHRP offers new insight into the reporter gene system for drug screening and intracellular signaling management and provides a precise, sustainable and affordable operating environment.","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":"19 1","pages":"37"},"PeriodicalIF":5.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12012966/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decellularized apple hypanthium as a plant-based biomaterial for cartilage regeneration in vitro: a comparative study of progenitor cell types and environmental conditions. 脱细胞苹果托杯作为体外软骨再生植物基生物材料：祖细胞类型和环境条件的比较研究。

IF 5.7 3区生物学

Journal of Biological Engineering Pub Date : 2025-04-22 DOI: 10.1186/s13036-025-00502-2

Mira Hammad, Justin Dugué, Eric Maubert, Catherine Baugé, Karim Boumédiene

{"title":"Decellularized apple hypanthium as a plant-based biomaterial for cartilage regeneration in vitro: a comparative study of progenitor cell types and environmental conditions.","authors":"Mira Hammad, Justin Dugué, Eric Maubert, Catherine Baugé, Karim Boumédiene","doi":"10.1186/s13036-025-00502-2","DOIUrl":"https://doi.org/10.1186/s13036-025-00502-2","url":null,"abstract":"Background: Decellularized plant tissues have been shown to enhance the integration and proliferation of human cells, demonstrating biocompatibility. These tissues are now being considered as valuable biomaterials for tissue engineering due to their diverse architectures and favorable cytocompatibility. In this study, we assessed decellularized apple hypanthium as a potential biomaterial for generating cartilage-like structures, utilizing four different progenitor cell types and varying environmental conditions in vitro.Results: Cell viability assays indicated integration and cell proliferation. Histological staining and gene expression analyses confirmed the synthesis and deposition of a cartilaginous extracellular matrix. Notably, hypoxia had varying effects on chondrogenesis based on the cell type. Among the progenitor cells evaluated, those derived from auricular perichondrium were particularly promising, as they differentiated into chondrocytes without requiring a low-oxygen environment. Additionally, our findings demonstrated that apple-derived biomaterials outperformed microencapsulation in alginate beads in promoting chondrogenesis.Conclusion: These results highlight the potential of plant-based biomaterials for the development of implantable devices for cartilage regeneration and suggest broader applications in tissue engineering and future clinical endeavors.","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":"19 1","pages":"38"},"PeriodicalIF":5.7,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12012941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-inflammatory activity of magnetic fields emitted by graphene devices on cultured human cells. 石墨烯装置发射的磁场对培养的人类细胞的抗炎活性。

IF 5.7 3区生物学

Journal of Biological Engineering Pub Date : 2025-04-21 DOI: 10.1186/s13036-025-00507-x

Sara Franceschelli, Federica De Cecco, Stefano Benedetti, Valeria Panella, Lorenza Speranza, Alfredo Grilli, Pierdomenico D'Andrea

{"title":"Anti-inflammatory activity of magnetic fields emitted by graphene devices on cultured human cells.","authors":"Sara Franceschelli, Federica De Cecco, Stefano Benedetti, Valeria Panella, Lorenza Speranza, Alfredo Grilli, Pierdomenico D'Andrea","doi":"10.1186/s13036-025-00507-x","DOIUrl":"https://doi.org/10.1186/s13036-025-00507-x","url":null,"abstract":"Background: Inflammation plays a key role in various diseases such as pancreatitis, cancer, and rheumatoid arthritis. Acute inflammation involves processes like vasodilation, increased vascular permeability, and leukocyte accumulation, which lead to cellular damage due to reactive oxygen species (ROS). Low-frequency electromagnetic fields (ELF-EMFs) have shown potential in reducing oxidative stress and inflammation. This study assesses the effectiveness of a new wearable device containing graphene quantum dots in reducing inflammation and oxidative stress in Jurkat T cells stimulated by lipopolysaccharide (LPS). The device is evaluated for its impact on ROS production and inflammation.Results: The results show that the device significantly lowers ROS levels and reduces the inflammatory response by decreasing pro-inflammatory cytokines such as IL-6, TNF-α, and IL-1β. Additionally, the device inhibits LPS-induced iNOS and COX-2 activity and modulates NF-κB signaling, indicating its potential as a therapeutic tool for managing inflammation and oxidative stress.Conclusion: These findings highlight the device's ability to combat inflammation, offering a non-invasive and effective approach for inflammatory diseases.","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":"19 1","pages":"36"},"PeriodicalIF":5.7,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12013070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144006699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0