用于软组织再生的三维针织可吸收聚(D, l -乳酸)(PLA)和聚己内酯(PCL)支架材料和生物学特性:从制造到体内性能

IF 6.5 3区 生物学 Q1 BIOCHEMICAL RESEARCH METHODS
Mélanie Dhayer, Vivien Barral, Damien Cleret, Amélia Jordao, Anne-Sophie Drucbert, Nicolas Germain, Sophie Dropsit, Patrice Maboudou, Salim Dekiouk, Stéphanie Brun, Christine Campagne, Éric Devaux, Pierre Guerreschi, Aurélie Cayla, Philippe Marchetti
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引用次数: 0

摘要

背景:软组织重建在整形外科和肿瘤学等领域是解决受损组织修复的关键。由生物可吸收共聚物,特别是聚(D, l -丙交酯)(PLA)和聚己内酯(PCL)制成的针织支架,具有组织工程所必需的机械和生物特性。本研究评估了熔融纺丝PLA和PCL多丝编织的三维编织支架在软组织工程中的应用。研究了PLA/PCL比例对编织支架结构、力学性能和生物反应的影响,以确定脂肪组织重建的最佳组成。结果:PLA/PCL共混物(PLA70/PCL30和PLA90/PCL10)制备的针织支架具有明显的力学和生物学特性。PCL含量较高的PLA70/PCL30支架具有较好的弹性和孔隙度,而PLA90/PCL10支架具有较好的结构完整性和刚度。生物实验证实了所有支架的体外生物相容性,无细胞毒性作用。尽管PLA70/PCL30的效果略有下降,但支架在体外支持成脂分化。血管化是明显的使用绒毛膜尿囊膜试验,其中血管形成和渗透观察,无论支架组成。在大鼠模型体内植入显示有效的脂肪细胞整合,结构稳定,炎症反应最小,PLA90/PCL10支架在血管化和更少的巨噬细胞浸润结缔组织方面优于PLA70/PCL30。结论:PLA/PCL编织支架具有可调节的力学性能和生物降解性,是增强移植物体积维持和改善长期预后的一种有前景的解决方案。PLA90/PCL10支架在结构稳定性和生物相容性之间取得了平衡,是脂肪组织重建的理想候选材料。这些发现强调了PLA/PCL支架在重建手术中的潜力。未来的研究应关注可扩展性和长期生物相容性,以促进临床翻译。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Material and biological characterization of 3D knitted bioresorbable poly (D,L-lactide) (PLA) and polycaprolactone (PCL) scaffolds for soft tissue regeneration: from fabrication to in vivo performance.

Background: Soft-tissue reconstruction is crucial in fields such as plastic surgery and oncology to address the repair of damaged tissues. Knitted scaffolds from bioresorbable copolymers, specifically poly(D,L-lactide) (PLA) and polycaprolactone (PCL), offer mechanical and biological properties that are essential for tissue engineering. This study assessed three-dimensional knitted scaffolds fabricated from melt-spun PLA and PCL multifilaments for soft tissue engineering applications. It examined the impact of the PLA/PCL ratio on the knitted scaffold structure, mechanical properties, and biological responses to determine the optimal composition for adipose tissue reconstruction.

Results: Knitted scaffolds fabricated with the PLA/PCL blends (PLA70/PCL30 and PLA90/PCL10) exhibited distinct mechanical and biological profiles. PLA70/PCL30 scaffolds with a higher PCL content showed enhanced elasticity and porosity, whereas PLA90/PCL10 scaffolds maintained better structural integrity and stiffness. Biological assays confirmed the biocompatibility of all scaffolds in vitro, with no cytotoxic effects. The scaffolds supported adipogenic differentiation in vitro, although PLA70/PCL30 exhibited slightly reduced efficacy. Vascularization was evident using chorioallantoic membrane assays, in which blood vessel formation and penetration were observed, regardless of the scaffold composition. In vivo implantation in rat models revealed effective adipocyte integration, structural stability, and minimal inflammatory response, with PLA90/PCL10 scaffolds outperforming PLA70/PCL30 in terms of vascularization and less macrophage infiltration of connective tissue.

Conclusion: PLA/PCL knitted scaffolds offer a promising solution for enhancing graft volume maintenance and improving long-term outcomes, with tunable mechanical properties and biodegradability. The PLA90/PCL10 scaffold is a superior candidate for adipose tissue reconstruction, balancing the structural stability with biological compatibility. These findings underscore the potential of PLA/PCL scaffolds for reconstructive surgery. Future studies should focus on scalability and long-term biocompatibility to facilitate clinical translation.

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来源期刊
Journal of Biological Engineering
Journal of Biological Engineering BIOCHEMICAL RESEARCH METHODS-BIOTECHNOLOGY & APPLIED MICROBIOLOGY
CiteScore
7.10
自引率
1.80%
发文量
32
审稿时长
17 weeks
期刊介绍: Biological engineering is an emerging discipline that encompasses engineering theory and practice connected to and derived from the science of biology, just as mechanical engineering and electrical engineering are rooted in physics and chemical engineering in chemistry. Topical areas include, but are not limited to: Synthetic biology and cellular design Biomolecular, cellular and tissue engineering Bioproduction and metabolic engineering Biosensors Ecological and environmental engineering Biological engineering education and the biodesign process As the official journal of the Institute of Biological Engineering, Journal of Biological Engineering provides a home for the continuum from biological information science, molecules and cells, product formation, wastes and remediation, and educational advances in curriculum content and pedagogy at the undergraduate and graduate-levels. Manuscripts should explore commonalities with other fields of application by providing some discussion of the broader context of the work and how it connects to other areas within the field.
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