Journal of Biological Engineering最新文献

{"title":"A novel device for in-situ on-demand hydrogen sulfide generation and delivery to increase tissue perfusion to chronic wounds.","authors":"Matthew Justus, Debra Friedrichsen, Bill Brooks, Grayson Silaski, Robert Beckius, Pierce Massie, Carolyn Pace, Deepali Kulkarni, Trevor Self, Mildred Mattox, Jeffrey Bray, Erin Risotto-Urbanowicz, Andrea Iturralde-Carillo, Jenna Marek, Brittany Coffman, Cristine Heaps, Heather Ferguson, Reza Shekarriz, Nancy Kanagy, Ross Clark","doi":"10.1186/s13036-026-00654-9","DOIUrl":"10.1186/s13036-026-00654-9","url":null,"abstract":"<p><p>BACKGROUND: There is significant evidence linking deficiency in endogenous hydrogen sulfide (H2S) to delayed healing of wounds. Systemic administration of H2S donors has been shown to markedly improve the healing rate of ischemic open wounds. This paper describes a novel H2S therapy approach, namely H2EALS™, as an exogenous supplement to accelerate healing. H2EALS™ employs a controllable process for in-situ conversion of a metal sulfide coating to H2S gas for safe and effective H2S treatment. METHODS: The core technology topically releases a targeted quantity of H2S molecules within the dressing for absorption into the open wound using control and delivery protocol specified by the end-user. Benchtop studies have evaluated precision in the rate and amount of H2S released and the degree of control, all required for safe treatment. In preclinical studies using rodent and swine models, the potential widespread dispersion of the H2S delivered was evaluated with an H2S sensing approach, while the physiological dose-response in terms of absorption and perfusion within the wound and periwound regions were examined using both H2S measurement and Laser Speckle Contrast Imaging (LSCI). RESULTS: Benchtop studies on H2EALS™ demonstrate clinically relevant performance durability and control over H2S dose administered. With low power requirement, high dosage control, and relatively low dose-to-dose variability, electroactive H2S sources with capacity of > 2 µmol/application were fabricated to last beyond a typical 14-day wound treatment period. In-vivo studies on open wounds in Sprague-Dawley rats treated at 25 to 100 nmol/dose indicated rapid tissue absorption of H2S gas. Real-time breath- and skin-emitted H2S measured on swine revealed no evidence of widespread dispersion when up to 500 nmol/dose was delivered directly to an 8-mm open wound. CONCLUSION: Our results emphasize the need for informed delivery and absorption of H2S to the target tissue and we demonstrate the potential of the H2EALS™ technology of safe and effective H2S treatment of open wounds and in acceleration of wound healing. The results are a partial contribution to evaluating the impact of H2EALS™ on wound healing acceleration and tissue repair. </p>","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13085374/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147390094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptive laboratory evolution with ethionine identifies novel genetic determinants for enhanced protein and methionine accumulation in Saccharomyces cerevisiae.","authors":"Tae Hoon Lee, Sang-Hun Do, Hyun-Jae Lee, Kun-Jae Lee, Jonghyeok Shin, Yong-Cheol Park, Sun-Ki Kim","doi":"10.1186/s13036-026-00652-x","DOIUrl":"10.1186/s13036-026-00652-x","url":null,"abstract":"<p><p>Enhancing methionine and protein content in Saccharomyces cerevisiae is essential for its use as single-cell protein. Here, we applied ethionine resistance-mediated adaptive laboratory evolution (ALE) to generate strains with improved resistance to this toxic methionine analog. Stepwise adaptation enabled growth at ethionine concentrations of up to 0.50 mM and yielded strains with progressively higher intracellular methionine levels and improved protein production efficiency. Whole-genome sequencing identified nonsynonymous SNPs in 32 genes, of which nine candidates were functionally validated. CRISPR/Cas9-based editing demonstrated that mutations in MDE1 and JJJ1 directly elevated free methionine levels, whereas most other mutations increased overall protein accumulation. Functional annotations linked these genes to RNA processing, protein degradation, methionine salvage, and amino acid uptake, highlighting RNA processing as a major target for global protein enhancement. These findings reveal that ethionine resistance-mediated ALE induces multifactorial adaptations. They also provide new insights into protein biosynthesis regulation and lay a foundation for future engineering of high-performance yeast strains.</p>","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13081643/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147372628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bakuchiol-loaded amphiphilic polymeric nanoparticles: enhancing stability and multifunctionality through encapsulation.","authors":"Dongseong Seo, Sohyeon Yu, Taeho Kim, Jonghyun Park, Hyungjun Kim, Daekyung Sung","doi":"10.1186/s13036-026-00644-x","DOIUrl":"10.1186/s13036-026-00644-x","url":null,"abstract":"<p><p>Bakuchiol (BK), a natural meroterpene isolated from Psoralea corylifolia, has gained significant attention as a functional cosmetic ingredient due to its antioxidant, anti-inflammatory, and antibacterial properties. However, its high hydrophobicity and chemical instability limit its practical application. In this study, we developed a robust and efficient delivery system for BK using polyglyceryl-10 laurate (PG10-L), an amphiphilic polymer, via a simple and scalable nanoprecipitation strategy. This approach helps address an important challenge in achieving stable long-term aqueous formulations of BK without relying on complex synthetic processes. The resulting BK-loaded nanoparticles (BK@NPs) exhibited a small particle size (< 200 nm), high encapsulation efficiency (≈ 99%), and remarkable colloidal stability for over 12 weeks. Notably, BK@NPs significantly enhanced biological efficacy compared to free BK, resulting in markedly improved antioxidant and antibacterial performance. Furthermore, the formulation demonstrated excellent cytocompatibility in NIH-3T3 murine fibroblasts along with effective suppression of intracellular reactive oxygen species (ROS). These findings highlight that the PG10-L-based nanoprecipitation strategy provides a robust and green platform to enhance the multifunctional potential of bakuchiol for advanced dermo-cosmetic applications.</p>","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13064261/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147344237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the impact of multinational collaborations on cultural understanding, health disparities, biomedical innovations, and professional development through project-based learning.","authors":"Breanna Kilgore, Nickolas A Gustavison, Alexandra Guiterrez-Vega, Ernesto Ibarra-Ramírez, Luis Estrada-Petrocelli, Jay Jesus Molino, Shilpa Drakshi, Anil S Bilimale, Devanshi Somaiya, Abhishek Appaji, Rogelio Garcia Contreras, Raj R Rao","doi":"10.1186/s13036-026-00646-9","DOIUrl":"10.1186/s13036-026-00646-9","url":null,"abstract":"<p><p>BACKGROUND: The criticality of global health was put on full display following the COVID-19 pandemic and its devastating effects on communities, irrespective of their socioeconomic status. Biomedical innovations that have been actively developed over the years played a critical role in combating the crisis and bringing communities together. However, even with significant technological advancements, solving global health issues with realistic solutions requires an intimate understanding of culture, socioeconomics, and educational competency, among other factors. The Biomedical Innovations for Global Impact course at the University of Arkansas, Fayetteville, provides students with the opportunity to focus on specific healthcare system problems and work collaboratively with students from other disciplines and other countries. By collaborating with institutions in Panama and India, students are provided the opportunity to address health care system challenges under the advisement of clinical and entrepreneurial mentors. This study focused on evaluating the course via pre- and post-course surveys completed by the students, focusing on their educational experience, personal growth, professional development, and cultural understanding. RESULTS: Student feedback on educational experience indicated a strong agreement that the course enhanced understanding of the design process as well as infrastructure challenges. Post-course analysis revealed an improvement in research skills, interdisciplinary communication, and real-world applications of both business and biomedical engineering principles. Approximately 85% of students agreed that the course improved their ability to engage in interdisciplinary groups while enhancing their understanding of global health. No significant shifts in the pre- and post-course responses were observed, which indicated that the high expectations of students were met. While most students recognized the value of engineering and entrepreneurial partnerships, some expressed interest in the application of these lessons on an international stage. The students’ cultural understanding prompts revealed a high agreement on its importance in global innovation. However, results revealed challenges in cross-cultural engagement and internal team communication, and active engagement. CONCLUSION: Qualitative responses revealed that students grew in empathy, cultural competence, and awareness of global health disparities. Despite the challenges that students experienced, many students appreciated the opportunity to apply their knowledge to explore diverse ideas and engage with real-world challenges as part of the course. The multicultural teams encouraged creativity and allowed students to broaden their horizons. In addition, the students had to learn to re-evaluate prior assumptions and increase their empathy, adaptability, and inclusiveness. </p>","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13101157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147344277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered chitosan/HP-β-CD hydrogel for targeted co-delivery of curcumin and berberine for the treatment of ulcerative colitis.","authors":"Leilei Li, Meilan Wang, Shiping Zhou, Yuanting Yang, Sileng Hu, Hongyan Li, Yuqin Li","doi":"10.1186/s13036-026-00621-4","DOIUrl":"10.1186/s13036-026-00621-4","url":null,"abstract":"<p><p>Ulcerative colitis (UC) presents considerable challenges in clinical treatment due to its multifaceted and complex nature. Efficacious therapy technique in developing an oral formulation that can encapsulate and deliver therapeutic drugs directly to the colon to diminish intestinal inflammation and restore the impaired intestinal barrier is of great interest. Herein, we developed a supramolecular hydrogel platform composed of 2-hydroxypropyl-β-cyclodextrin/chitosan in which curcumin and berberine (HCD/CH-CUR-BBR) are loaded in the hydrophobic cavity of HP-β-CD. BET surface area was measured to be 198.3 m²g^- 1 with a pore diameter of 3.56 μm which improved in vitro drug release. The results demonstrate that the HCD/CH-CUR-BBR hydrogel leverages pH sensitivity to achieve sustained drug release in the colon while promoting targeted delivery to colonic tissues. Interestingly, in vitro and in vivo evaluation confirms that HCD/CH-CUR-BBR hydrogel formulation alleviates the progression of UC via downregulating IL-6 and TNF-α expression. Subsequently, in vivo anti-colitis investigation demonstrated notable enhancement in tight junction protein expression, underscoring anti-inflammatory efficiency of CUR and BBR loaded HCD/CH hydrogel. These findings indicate that the HCD/CH hydrogel infused with CUR and BBR displayed potential as an effective targeted drug delivery strategy for UC treatment.</p>","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13059576/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147317125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of AS1411 aptamer-conjugated chitosan-carbon dot nanocarriers for targeted drug delivery and fluorescent imaging in breast cancer therapy.","authors":"Mohammad Jafar Mantashlou, Effat Alizadeh, Sevil Vaghefi Moghaddam, Neda Saraygord Afshari, Amir Noorbakhsh","doi":"10.1186/s13036-026-00643-y","DOIUrl":"10.1186/s13036-026-00643-y","url":null,"abstract":"<p><p>Theranostic nanomedicine, combining therapy with diagnostic imaging, offers a powerful strategy for real-time monitoring and targeted treatment of cancer. In the current study, we developed a pH-sensitive delivery system based on chitosan (CS) and poly caprolactone-poly ethylene glycol- poly caprolactone (PCL-PEG-PCL) copolymer, which was conjugated to the AS1411 Aptamer and tagged with carbon dots (CDs), abbreviated as DOX/P/CS-CD-Apt. Then, the theranostic potential of DOX/P/CS-CD-Apt in carrying DOX to MCF-7 breast cancer cells was evaluated both in vitro and in vivo. CDs were synthesized via a one-step hydrothermal method and chemically conjugated to the CS backbone along with AS1411 using EDC/NHS chemistry. On the other hand, DOX is encapsulated into the final carrier through the double emulsion-solvent evaporation method. The nanocarrier was characterized using FT-IR, FESEM, XPS, XRD, TEM, DLS, and zeta potential. Our results represented that DOX/P/CS-CD-Apt were uniform spherical morphology, high drug encapsulation, and controlled release under acidic conditions. Fluorescence microscopy revealed cytoplasmic entrance of DOX/P/CS-CD-Apt in MCF-7 cells, indicating effective nucleolin-mediated uptake. MTT assays and apoptosis evaluation demonstrated the higher cytotoxicity and apoptotic effects compared to free DOX or non-targeted formulations. Moreover, in vivo PET imaging confirmed the selective accumulation of nanoparticles at the tumor site in a 4T1 breast cancer model, along with a notable reduction in tumor size. These findings highlight DOX/P/CS-CD-Apt as a promising theranostic platform for targeted breast cancer therapy with integrated imaging capability.</p>","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13041373/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147317095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolvoid: a genetic algorithm for shaping optimal cellular constructs.","authors":"Piera Mancini, Flavio Fontana, Ermes Botte, Chiara Magliaro, Francesco Pampaloni, Arti Ahluwalia","doi":"10.1186/s13036-026-00642-z","DOIUrl":"10.1186/s13036-026-00642-z","url":null,"abstract":"<p><strong>Background: </strong>Accurate cellular models are critical for understanding tissue function and accelerating drug discovery. While in vitro systems like spheroids and organoids are widely used, they are costly, low-throughput, and often lack reproducibility. Here, we introduce Evolvoid, a computational pipeline based on genetic algorithms that generates virtual 3D spheroid-like cell constructs with optimized morphologies by simulating key biophysical principles - such as thermodynamic principles, nutrient transport and their uptake by cells.</p><p><strong>Results: </strong>By integrating finite element simulations with evolutionary principles, our in silico platform evolves populations of randomly generated shapes, iteratively selecting individuals that best satisfy a biophysically informed fitness function. The fitness function encodes selection pressures based on general biophysical constraints - namely, the minimization of surface energy and the maintenance of cell viability under variable oxygen conditions. The Shannon entropy is used to track genome complexity over generations. In consistence with evolutionary dynamics, the complexity increases progressively. Moreover, albeit based on basic biophysical optimization rules, the fittest individuals generated by Evolvoid closely resemble the shape and size of cellular spheroids obtained in vitro.</p><p><strong>Conclusion: </strong>Evolvoid is modular, scalable, and tunable to different construct sizes, cell types, or culture conditions, and thus provides a versatile platform for designing and optimizing 3D cellular models entirely in silico. It offers a foundation for developing high-fidelity, cost-effective digital shape twins of biological systems, supporting the advancement of lab-on-a-laptop technologies and new approach methodologies, thereby reducing the reliance on in vitro and animal models.</p>","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13049953/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147317121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of PGCL/AP conductive composite scaffold combined with electrical stimulation in neural tissue engineering.","authors":"Longchuan Zhu, Hongye Yan, Hongru Li, Su Pan, Hanwen Yang, Zhe Zhu, Zhiping Qi","doi":"10.1186/s13036-026-00620-5","DOIUrl":"10.1186/s13036-026-00620-5","url":null,"abstract":"<p><p>Nerve regeneration and axonal growth can be significantly enhanced by electrical stimulation (ES) using conductive polymers. But in addition to ES, nerve cell function and how they react to substrate conductivity are also essential factors in nerve repair. As a result, ES, in conjunction with appropriate materials (such as tissue scaffolds), has been used to treat nerve damage and has demonstrated significant promise for peripheral nerve regeneration. In this work, electrospinning was used to create conductive composite scaffolds made of polyglycolic acid@polycaprolactone/aniline pentamer (PGCL/AP). We later verified that PC12 cells cultured on these scaffolds exhibited improved migration, proliferation, and vitality upon electrical stimulation. Furthermore, In the established 10-mm peripheral nerve injury model, the PAP + ES group achieved outcomes comparable to the autograft group in the assessment of functional and morphological recovery, encompassing SFI, myelination rate, and gastrocnemius muscle evaluation.</p>","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13041166/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147306212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating the metabolic landscape of Bacillus velezensis WY-65 in basic and cocktail nutrient media: a biocontrol approach against peanut web blotch.","authors":"Taswar Ahsan, Chaoqun Zang, Xue Pei, Yuqian Huang, Chunhao Liang, Muhammad Irfan","doi":"10.1186/s13036-026-00640-1","DOIUrl":"10.1186/s13036-026-00640-1","url":null,"abstract":"<p><p>BACKGROUND: In this study, the cocktail medium significantly enhanced antifungal metabolite production, thereby improving the suppression of peanut web blotch caused by Peyronellaea arachidicola. RESULTS: A novel antagonistic bacterial strain was isolated from cultivated peanut soil and identified as Bacillus velezensis WY-65. Additionally, the metabolomic profile of strain WY-65 was investigated under two fermentation conditions: basic fermentation medium (BFM) and cocktail fermentation medium (CFM). In vitro assays revealed that the cell-free supernatant (CFS) from WY-65 cultured in CFM inhibited P. arachidicola by 87.68%, whereas the CFS from BFM inhibited P. arachidicola by 61.42%. Greenhouse trials targeting peanut web blotch demonstrated that CFM achieved a disease control efficiency of 92%, whereas BFM exhibited a control efficiency of 86%. Treatment with CFM reduced the disease incidence to 8.71%, and BFM treatment resulted in a disease incidence of 15%. In contrast, the control group presented a disease incidence of 95%. CFS significantly impacted the efflux pump and caused ROS in P. arachidicola. Using untargeted LC‒MS/UHPLC-based metabolomics, we identified distinct metabolic signatures influenced by medium composition, revealing an enrichment of bioactive secondary metabolites, including 3-indoleacrylic acid and L-tryptophan, in CFM. KEGG differential abundance (DA) analysis revealed that strain WY-65 cultured in CFM underwent broad metabolic reprogramming, with significant enrichment in amino acid biosynthesis and the phenylalanine, tyrosine, and tryptophan synthesis pathways. Pathway topology analysis further revealed that glycine, serine, and threonine metabolism and phenylalanine, tyrosine, and tryptophan synthesis pathways had the greatest impact and significance, highlighting their central roles in stress adaptation and antimicrobial precursor biosynthesis. In vitro confirmatory tests with synthetic chemicals confirmed the bioactivity of these compounds. At 200 μL, both 3-indoleacrylic acid and L-tryptophan exhibited peak antifungal activity (91% and 89%, respectively), whereas their activities at 150 μL (87% vs. 75%) and 300 μL (89% vs. 80%) were comparatively lower. CONCLUSION: Our findings provide insight into medium-induced metabolomic modulation in strain WY-65 and highlight its potential as a robust biocontrol agent for sustainable peanut web blot disease management. CLINICAL TRIAL: Not applicable. </p>","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13040968/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147283893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tetramethylbenzidine loaded PVA nanofibers strip for visual detection of hydrogen peroxide via Cu²⁺ catalysis and its applications.","authors":"Marwa A Aleem, Eman A Bahgat, Soad S Abd El-Hay, Samar A Salim","doi":"10.1186/s13036-026-00632-1","DOIUrl":"10.1186/s13036-026-00632-1","url":null,"abstract":"<p><p>BACKGROUND: Advancements in sensor technology have facilitated the development of cost-effective and highly sensitive sensors which have significantly improved the detection of biological analytes in various fields. This study presents a novel approach for the detection of hydrogen peroxide (H₂O₂), a critical analyte in both biological and environmental systems. A simple and sensitive colorimetric sensing platform was developed based on polyvinyl alcohol (PVA) nanofibers loaded with tetramethylbenzidine (TMB). RESULTS: The nanofibers were characterized using scanning electron microscopy, confirming the formation of smooth, spindle-shaped fibers with diameters from 324 to 551 nm. The colorimetric detection method utilized TMB, which undergoes oxidation in the presence of H2O2 catalyzed by Cu2 + resulting in the formation of a characteristic blue color. It revealed a linear increase in absorbance corresponding to H2O2 concentrations from 3.125 to 100 µM, with a low detection limit of 2.60 µM. The system exhibited excellent specificity and selectivity for H₂O₂ with a visual color gradient scale over common biological interferents such as glutathione, L-cysteine, urea, ascorbic acid, or various ions at a concentration of 100 µM. The new colorimetric sensor was successfully used to determine the H2O2 level in human urine from healthy subjects demonstrated excellent recovery rates (96–103%). SIGNIFICANCE: This work introduces a simple, cost-effective, and highly selective colorimetric sensor, which is capable of rapid, on-site H₂O₂ detection without reliance on complex instrumentation. Also, the electrospun PVA/TMB exhibits strong potential of nanofibers to be used as a valuable tool for medical diagnostics, environmental monitoring and other biosensing applications. </p>","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":" ","pages":""},"PeriodicalIF":6.3,"publicationDate":"2026-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13041158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147283899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}