Journal of Biological Engineering最新文献

{"title":"Elucidating the metabolic landscape of Bacillus velezensis WY-65 in basic and cocktail nutrient media: a biocontrol approach against peanut web blotch.","authors":"Taswar Ahsan, Chaoqun Zang, Xue Pei, Yuqian Huang, Chunhao Liang, Muhammad Irfan","doi":"10.1186/s13036-026-00640-1","DOIUrl":"10.1186/s13036-026-00640-1","url":null,"abstract":"","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2026-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13040968/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147283893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tetramethylbenzidine loaded PVA nanofibers strip for visual detection of hydrogen peroxide via Cu²⁺ catalysis and its applications.","authors":"Marwa A Aleem, Eman A Bahgat, Soad S Abd El-Hay, Samar A Salim","doi":"10.1186/s13036-026-00632-1","DOIUrl":"10.1186/s13036-026-00632-1","url":null,"abstract":"","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2026-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13041158/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147283899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amplification-free CRISPR/Cas13a-based electrochemical biosensor for the detection of circulating tumor RNA using RNA-3Ag⁺ probes on Au porous-lattice nanoelectrode.","authors":"Yu-Rim Lee, Myeong-Jun Lee, Sang-Nam Lee, Jeong-Woo Choi","doi":"10.1186/s13036-026-00641-0","DOIUrl":"10.1186/s13036-026-00641-0","url":null,"abstract":"","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2026-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13037025/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147275694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioreactor expansion of human neural progenitor cells for exosome scalable production and miRNA-engineering.","authors":"Miriam Corraliza-Gomez, Marta Ianni, Diogo E S Nogueira, William O S Salvador, Mafalda Miguéis, Monica Garcia-Alloza, Carlos A V Rodrigues, Joaquim M S Cabral, Dora Brites","doi":"10.1186/s13036-025-00597-7","DOIUrl":"10.1186/s13036-025-00597-7","url":null,"abstract":"","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":"20 1","pages":"37"},"PeriodicalIF":6.5,"publicationDate":"2026-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12922370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146226824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of CRISPR-Cas12a/Cas13a dual enzyme cleavage technology in the differential diagnosis of Chikungunya virus and Dengue virus.","authors":"Tong Jiang, Zijing Guo, Zhuo Wang","doi":"10.1186/s13036-026-00639-8","DOIUrl":"10.1186/s13036-026-00639-8","url":null,"abstract":"","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2026-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13023200/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146226889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesized uricase with dual-terminus zwitterionic EKylation for prolonged elimination half-life and reduced immunogenicity.","authors":"Da Xu, Hongyi Cai, Tianyang Huang, Ke Bi, Zhinan Xu, Yi He, Gang Cheng, Shengfu Chen","doi":"10.1186/s13036-026-00636-x","DOIUrl":"10.1186/s13036-026-00636-x","url":null,"abstract":"","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2026-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13020289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146219525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Controlled reductive unfolding of BSA-α under pH stimuli and its supramolecular assembly with aminated gum acacia nanocarriers for targeted gemcitabine delivery in lung cancer therapy.","authors":"Yuan Li, Ping Zhang, Yong Ma, Chenle Jia","doi":"10.1186/s13036-026-00631-2","DOIUrl":"10.1186/s13036-026-00631-2","url":null,"abstract":"","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2026-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13005361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146194568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodetection of Mycobacterium tuberculosis: nano-biosensors in detection; from principles to recent progresses.","authors":"Hossein Jooya, Sama Yavari, Maryam Meskini, Seyyed Mohammad Amin Mousavi-Sagharchi, Seyed Davar Siadat, Maryamosadat Mavaei","doi":"10.1186/s13036-026-00638-9","DOIUrl":"10.1186/s13036-026-00638-9","url":null,"abstract":"","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2026-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12998067/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146180110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Verification and application of an automated real-time antimicrobial susceptibility testing system: for accelerated antibiotic susceptibility testing and high-throughput screening of antibiotic synergistic effects.","authors":"Yunxiang Xiao, Xinyu Yan, Zhenzhong Liu, Jie Fu, Zhihua Wang, Dianzheng Jiang, Heran Zhu, Yue Sun, Huili Xia, Changyan Xue","doi":"10.1186/s13036-026-00630-3","DOIUrl":"10.1186/s13036-026-00630-3","url":null,"abstract":"","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2026-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12977749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146137278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protein-inducible ribosomal frameshifting enables programmable translational control for genetic circuit design in Escherichia coli.","authors":"Seongho Hong, Yelin Lim, Hansol Kang, Jongmin Kim","doi":"10.1186/s13036-026-00629-w","DOIUrl":"10.1186/s13036-026-00629-w","url":null,"abstract":"<p><strong>Background: </strong>Programmed ribosomal frameshifting (PRF) is a translational mechanism that enables the ribosome to shift reading frames and access alternative coding sequences. PRF occurs naturally in a wide range of organisms, including viruses, bacteria, and eukaryotes, where it supports compact encoding and stoichiometric control of protein expression. Despite the great potential of PRF in synthetic circuit designs, a broader adoption of PRF in circuit designs has been hampered by rather strict sequence constraints and structural requirements.</p><p><strong>Results: </strong>This work introduces a synthetic translational regulatory platform, protein-inducible ribosomal frameshifting (PIRF), by integrating aptamer-protein interactions with a - 1 PRF motif to enable regulated translation in Escherichia coli. PIRF modules respond to intracellular RNA-binding proteins such as PP7 and MS2, triggering frameshifting in a condition-dependent manner. PIRF could be used to program logic gate operations through frame-dependent translation and enable multilayered regulation in synthetic circuits. Further, the flexible PIRF designs enable reading frame-dependent control of fusion protein expression, protein aggregation, and periplasmic localization via strategic positioning of peptide tags and protein coding sequences. While PIRF enabled regulated frameshifting and could be flexibly reconfigured for a variety of circuits and applications, a measurable level of basal frameshifting was often observed, which may require additional strategies for further optimization in the future. Together, PIRF supports applications in programmable and logical control of downstream protein expression, including condition-dependent aggregation and regulated subcellular localization.</p><p><strong>Conclusions: </strong>PIRF provides a compact and genetically encoded strategy for programmable protein-level regulation, expanding the synthetic biology toolkit for translational control, biosensing and biotherapeutics.</p>","PeriodicalId":15053,"journal":{"name":"Journal of Biological Engineering","volume":" ","pages":""},"PeriodicalIF":6.5,"publicationDate":"2026-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12977422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146137148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}