Food Science & Nutrition最新文献

{"title":"Correction to \"Boscisucrophage: A Natural SGLT1/2 Inhibitor From <i>Boscia senegalensis</i> for Managing Type 2 Diabetes\".","authors":"","doi":"10.1002/fsn3.71859","DOIUrl":"https://doi.org/10.1002/fsn3.71859","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1002/fsn3.71621.].</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"14 ","pages":"e71859"},"PeriodicalIF":3.8,"publicationDate":"2026-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroprotective Effects of Carnosine Against Corticosterone-Induced Depression and Memory Impairment.","authors":"Muneezah Elahi, Noreen Samad, Ali Irfan, Natasha Manzoor, Muhammad Zeeshan Ahmed, Emilio Mateev, Yousef A Bin Jardan","doi":"10.1002/fsn3.71837","DOIUrl":"https://doi.org/10.1002/fsn3.71837","url":null,"abstract":"<p><p>Corticosterone (Crt) is reported to induce oxidative stress in experimental animals. Carnosine (Crn), a well-known dipeptide antioxidant synthesized endogenously from β-alanine and L-histidine, neutralizes oxidative stress. The current study examines the influences of Crn on Crt-induced behavioral alterations (depressive-like behaviors and cognitive performance), oxidative damage, antioxidant enzymes activity, inflammatory markers, serotonin metabolism, and histopathology in female rats. Thirty-six animals were allocated to six groups (<i>n</i> = 6): (i) vehicle (vh) + vh (1 mg/kg) (ii) vh + Crn (20 mg/kg), (iii) vh + Crn (50 mg/kg), (iv) vh + Crt (20 mg/kg) (v) Crt + Crn (20 mg/kg), and (vi) Crt + Crn (50 mg/kg). All respective doses were given intraperitoneally (i.p) for 2 weeks. After treatment, behavioral testing was done via the Tail suspension test (TST) for depressive-like behavior and the Morris Water Maze (MWM) for spatial memory. Crn treatment had significant effects on Crt-induced behavioral impairments, such as depressive-like behavior and cognitive dysfunction. After completing behavioral tests, the rats underwent decapitation, and the hippocampus was separated for further biochemical and neurochemical analyses. Outcomes revealed that Crn mitigates depressive-like behaviors and cognitive dysfunction. Crn reduces oxidative stress and inflammatory cytokine levels while improving antioxidant enzyme activity, restoring cholinergic and serotonergic transmission, and brain (hippocampus) morphology following Crt-administration. The in silico analyses also demonstrate its strong binding affinity with monoamine oxidases (MAO) A and B, with an energy of -7.1 and -6.7 kcal/mol, respectively. In conclusion, the present results showed that Crn possesses strong antioxidant properties and reduced Crt-induced depressive-like behavior and memory impairment due to its effective abilities as antioxidant and neuromodulator. Supplementation with Crn as a dietary component may provide protective benefits against Crt-induced depression-like behavior and memory impairment.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"14 ","pages":"e71837"},"PeriodicalIF":3.8,"publicationDate":"2026-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13136518/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Functional Potential of Date (<i>Phoenix dactylifera</i>) Seed Bioactives in Modulating Gut Microbiota in Diabetic Rats.","authors":"Nouf Abdullah Alharbi, Saleh A Asanie, Asmahan A Ali, Aeshah Alhosain, Tarfa Albrahim, Thamer Aljutaily, Huda Aljumayi, Isam A Mohamed Ahmed, Belal M Mohammed, Nazeha A Khalil","doi":"10.1002/fsn3.71841","DOIUrl":"https://doi.org/10.1002/fsn3.71841","url":null,"abstract":"<p><p>Date fruits (<i>Phoenix dactilyfera</i> L.) are widely produced with huge wasted by-products (seeds/pits and fibers) in remarkable nutritional and economic potential value. This study aims to examine the impact of the functional properties of date seeds on health status and gut microbiota (GM) modulation in diabetic rats. Collected samples were dried and used in powdered form (DSP) for chemical compositions. Diabetic rats were then fed with this (5%, 10%, and 15%) versus control healthy group. Blood samples were used for glucose (BG) with lipid profiles (cholesterol; CHO), triglycerides (TG), high-density lipoprotein (HDL) and low-density lipoprotein (LDL). Additionally, kidney (Urea, Criatinine) and histological function and GM were measured. Data indicated that DSP contain crude fiber, total sugar, and crude fat about 77%, 6%, and 6% respectively. Additionally, potassium was the highest while phosphorus shows the lowest (31.53 ± 1.19 vs. 2.48 ± 0.31 g/100 g DSP). Iron, calcium, sodium, and magnesium were 14.29 ± 0.59, 12.53 ± 0.49, 9.23 ± 0.22, and 7.60 ± 0.42 g/100 g DSP respectively. The 10% and 15% DSP supplementations affected the GM; <i>Clostridium histolyticum</i> reductions versus <i>Bifidobacteria, Lactobacillus</i> enhancement. Also, kidney functions and lipid profile improved especially at 15% supplementations (CHO and TG decrease vs. HDL-c increase). Valorization of date seed by-products indicates their potential health effects on lipids and kidney functions among diabetic models influenced by their antioxidant, mineral and fiber levels in association to GM composition. However, further studies are needed on humans with sustainable utilization manner of food waste.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"14 5","pages":"e71841"},"PeriodicalIF":3.8,"publicationDate":"2026-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13136596/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147835733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Evaluation of Fructus Tsaoko, Seeds of Tsaoko Fructus and Ginger Made Seeds of Tsaoko Fructus Based on Drug-Like Compounds and Aroma Analysis.","authors":"Han Chen, Meiquan Yang, Weize Yang, Mingju Qi, Zongliang Xu, Tianmei Yang, Jinyu Zhang","doi":"10.1002/fsn3.71834","DOIUrl":"https://doi.org/10.1002/fsn3.71834","url":null,"abstract":"<p><p>Fructus tsaoko (CG) is the dried fruit of <i>Amomum tsaoko</i> Crevost et Lemaire. seeds of Tsaoko Fructus (QC) and ginger made seeds of Tsaoko Fructus (JZ) are two important decoction pieces derived from CG, which are rich in various chemical constituents. In this study, untargeted metabolomics combined with principal component analysis (PCA) was employed to establish models for analyzing and evaluating the drug-likeness of CG, QC, and JZ. Meanwhile, the aroma characteristics and volatile components of these samples were analyzed using relative odor activity values (ROAVs) and quantitative descriptive analysis (QDA). GC-MS and LC-MS analyses identified 1625 and 1145 metabolites, respectively. Further screening revealed 108 metabolites with drug-like properties and 44 aroma-related compounds. The dominant drug-like components were flavonoids and heterocyclic compounds. Paozhi (traditional Chinese herbal processing) mainly affected the relative contents of flavonoids and lignans. The drug-likeness decreased in the order of CG < QC < JZ. Among the aroma components, ketones and heterocyclic compounds contributed most to the overall aroma. CG was characterized by a pungent aroma with a hint of green notes, and its key aroma components were 3-octen-2-one, cyclohexanone, 2,2,6-trimethylcyclohexanone, and (5Z)-octa-1,5-dien-3-one. QC was characterized by a roasted aroma accompanied by fatty, green, and pungent notes, with 2-methoxy-3,5-dimethylpyrazine, (5Z)-octa-1,5-dien-3-one, and 3-octen-2-one as the key aroma components. JZ was characterized by a pungent aroma with a hint of green notes, and its key aroma components were 1-nonen-3-one, cyclohexanone, 2,2,6-trimethylcyclohexanone, and 2-methoxy-3-(2-methylpropyl) pyrazine. This study provides new insights into the metabolic changes of CG, QC, and JZ, laying a theoretical foundation for their more refined utilization and high-quality processing.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"14 ","pages":"e71834"},"PeriodicalIF":3.8,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147812778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Lactiplantibacillus plantarum</i> CQPC03 Reduces Carrageenan-Induced Thrombosis in Mice via Controlling the Flora in the Gut.","authors":"Xin Zhao, Kejia Wang, Tianyi Chen, Kai Ma, Rongming Zhang, Yongling Ou, Zhiping Kuang","doi":"10.1002/fsn3.71842","DOIUrl":"https://doi.org/10.1002/fsn3.71842","url":null,"abstract":"<p><p>This investigation reports the effects of <i>Lactiplantibacillus plantarum</i> CQPC03 (LP-CQPC03) on preventing thrombus formation and reducing intestinal oxidative stress and inflammation in a carrageenan-induced mouse thrombosis model. High-throughput 16S rRNA sequencing determined the composition of intestinal microorganisms. Biochemical reagents, section observations, and quantitative polymerase chain reaction (qPCR) identified mouse serum and tissue-related markers. Experimental findings show that LP-CQPC03 enhances activated partial thromboplastin time (APTT) and decreases thrombin time (TT), fibrinogen (FIB), and prothrombin time (PT). LP-CQPC03 also significantly reduces black tail severity in thrombotic mice. Moreover, LP-CQPC03 lowers malondialdehyde (MDA), tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), nuclear factor kappa-B (NF-κB), and interleukin-1 beta (IL-1β) levels in thrombotic mouse serum, while increasing superoxide dismutase (SOD) and catalase (CAT) activities. Hematoxylin and eosin (H&E) pathological analysis reveals that LP-CQPC03 lessens tissue damage caused by tail vein thrombosis. In the colon tissues of thrombotic mice, LP-CQPC03 up-regulates the mRNA expression of copper/zinc superoxide dismutase (Cu/Zn-SOD), manganese superoxide dismutase (Mn-SOD), and CAT, while down-regulating NF-κB p65, IL-6, TNF-α, and interferon gamma (IFN-γ). In tail vein vascular tissues, LP-CQPC03 also suppresses the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin. Gut microbiota sequencing results show that LP-CQPC03 increases the population of beneficial bacteria and decreases harmful ones. These findings demonstrate that LP-CQPC03 prevents thrombosis in mice, reduces oxidative stress and intestinal inflammation, and regulates gut microbiota by increasing beneficial bacteria. A high concentration of LP-CQPC03 shows a better effect, comparable to heparin.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"14 ","pages":"e71842"},"PeriodicalIF":3.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135114/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147812718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary Polyphenols in Non-Communicable Chronic Diseases: Neuro-Enteric Mechanisms, Multi-Omics Biomarkers and Translational Opportunities.","authors":"Adnan Akif, Jannatul Wahid Munami, Rajib Das, Nusrat Jahan Shawon","doi":"10.1002/fsn3.71856","DOIUrl":"https://doi.org/10.1002/fsn3.71856","url":null,"abstract":"<p><p>Polyphenols from plant foods (tea, cocoa, berries, grapes, and extra-virgin olive oil) modulate oxidative stress, inflammation, vascular function, and the gut microbiome-axes central to non-communicable chronic diseases (NCCDs) that involve the brain and enteric nervous system (ENS). Recent randomized trials and longitudinal studies report modest but reproducible benefits on cognitive domains and vascular/endothelial function with berry/grape extracts, matcha/green tea, and high-polyphenol extra-virgin olive oil; effects appear stronger in older adults or those with metabolic risk. Complementary evidence in irritable bowel syndrome (IBS)-a prototypical gut-brain disorder-suggests polyphenol-based combinations (often with probiotics/fiber) can improve quality of life and inflammatory markers, supporting enteric-central crosstalk. Emerging genetics (Mendelian randomization) and multi-omics readouts strengthen causal inferences for tea polyphenols in neurodegeneration-adjacent outcomes and outline mechanistic mediators (endothelial/BBB function, cytokine tone, microbiome-derived metabolites). Key gaps remain: heterogeneous formulations/doses, limited head-to-head trials, sparse target engagement biomarkers, and uncertain durability after discontinuation. We synthesize clinical and mechanistic advances, propose a standardized biomarker set (neurocognitive, endothelial, immune, and microbiome-metabolome), and outline designs for mechanism-anchored RCTs that integrate ENS endpoints with brain outcomes to translate associative signals into precision nutrition strategies for NCCDs.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"14 ","pages":"e71856"},"PeriodicalIF":3.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135109/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147812791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of <i>Mentha piperita</i> Essential Oil and Its Nanoemulsion on Microbial Growth, Physicochemical, and Organoleptic Properties of Mango Yogurt During Refrigerated Storage.","authors":"Fatemeh Chehri, Nafiseh Davati, Mostafa Karami","doi":"10.1002/fsn3.71845","DOIUrl":"https://doi.org/10.1002/fsn3.71845","url":null,"abstract":"<p><p>Fruit yogurts are more susceptible to microbial contamination than plain yogurts due to the incorporation of fruit ingredients, increasing the need for natural preservatives. This study aimed to evaluate the effects of <i>Mentha piperita</i> essential oil (MEO) and <i>Mentha piperita</i> essential oil nanoemulsion (MPON) as effective natural preservatives on microbial growth, physicochemical properties, and sensory attributes of mango yogurt during refrigerated storage. Gas chromatography-mass spectrometry analysis identified menthol and l-menthone as the major constituents of MEO. MPON exhibited significantly higher antioxidant activity (IC⁵⁰ = 15 μg/mL) compared to MEO (IC⁵⁰ = 40 μg/mL) and demonstrated enhanced antimicrobial efficacy against foodborne microorganisms. The results of minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) showed that <i>Pseudomonas aeruginosa</i> ATCC 9027 (MIC_MEO = 6000, MBC_MEO = 24,000, MIC_MPON = 2080, MBC_MPON = 4160 μg/mL) was the most resistant bacterium, and <i>Bacillus cereus</i> ATCC 11778 (MIC_MEO = 750, MBC_MEO = 1500, MIC_MPON = 520, MBC_MPON = 520 μg/mL) was the most sensitive. Incorporation of MEO, and particularly MPON, into mango yogurt significantly reduced microbial growth (<i>p</i> < 0.05), improved physicochemical stability during storage, and maintained desirable sensory properties. These findings indicate that a <i>Mentha piperita</i> essential oil nanoemulsion can serve as an effective natural preservative to enhance the overall quality and storage stability of fruit yogurts, supporting its potential application in dairy systems.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"14 ","pages":"e71845"},"PeriodicalIF":3.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147812853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protocatechuic Acid Alleviates D-Gal-Induced Renal Senescence and Injury in Mice by Regulating Taurine Metabolism.","authors":"Songhao Tian, Tao Chen, Feng Gao, Lulu Xu, Jiarui Zhao, Yuyao Du, Zhihua Zhao","doi":"10.1002/fsn3.71826","DOIUrl":"https://doi.org/10.1002/fsn3.71826","url":null,"abstract":"<p><p>Protocatechuic acid (PCA), a natural phenolic compound with antioxidant and anti-inflammatory properties, has been proposed as a potential therapeutic agent against aging-related diseases. This study investigated the protective effects of PCA on D-galactose (D-gal)-induced renal aging in mice. PCA administration significantly improved renal function, alleviated oxidative stress and inflammatory responses, and ameliorated histopathological abnormalities. Biochemical analyses revealed reductions in serum AGEs, β-galactosidase, creatinine, and blood urea nitrogen, accompanied by restoration of antioxidant enzyme activities and suppression of proinflammatory cytokines. Transcriptomic and metabolomic profiling further demonstrated that PCA reversed D-gal-induced molecular alterations, with integrated multi-omics analysis identifying taurine and hypotaurine metabolism as the key pathway mediating its renoprotective effects. Western blot validation confirmed that PCA regulates CSAD, an enzyme essential for taurine biosynthesis. Collectively, these findings provide novel mechanistic insights into renal aging and highlight PCA as a promising natural agent for delaying kidney senescence through modulation of taurine metabolism.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"14 5","pages":"e71826"},"PeriodicalIF":3.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135117/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147813060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Effect of African Walnut Oil and Docosahexaenoic Acid on Hematological and Oxidative Stress Markers of Female Wistar Rats With 7,12-Dimethylbenz[a]anthracene-Induced Breast Cancer.","authors":"Fabrice Tonfack Djikeng, Carister Nchangnwi, Jean Paul Chedjou, Hilaire Macaire Womeni","doi":"10.1002/fsn3.71821","DOIUrl":"https://doi.org/10.1002/fsn3.71821","url":null,"abstract":"<p><p>This work analyzed the effect of African walnut oil (AWO) on tumor size, hematological and oxidative stress markers in female rats with 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancer in comparison to docosahexaenoic acid (DHA). Eighteen rats with developed tumors were randomized into three groups of six rats each: the negative control, the positive control and test group. Six rats in which cancer was not induced served as the normal group. The four groups, respectively received distilled water (250 mg/kg), DHA (125 mg/kg), African walnut oil (1000 mg/kg), and distilled water by gavaging every day for 28 days. Tumor size was measured. The rats were sacrificed on the 29th day. The blood was collected, and part of it was used to prepare the serum. Organs of interest were used to prepare organ homogenates. Hematological markers were measured on the blood. The serum and organ homogenates were analyzed for their protein and oxidative stress markers. Results showed significantly (<i>p</i> < 0.001) higher tumor size in the negative control group (8.21 cm³) compared to the groups taking DHA (4 cm³) and AWO (5.37 cm³). Data from the hematological study showed that DHA and AWO oil increased white blood cells by 30.22%-88.83%, DHA increased platelets by 33.33% and AWO increased mean corpuscular hemoglobin in the blood by 32.94%. Rats that received DMBA presented significantly lower (<i>p</i> < 0.001) red blood cells (2.08-3.82 × 10¹²/L) and hemoglobin (5.10-7.30 g/dL) levels compared to the normal group (7.9 × 10¹²/L and 13.06 g/dL, respectively). The protein content of organ homogenate was significantly (<i>p</i> < 0.01) lower in the negative control group (0.16-2.92 g/dL) compared to other groups (~1.44-4.88 g/dL). Analysis of oxidative stress markers generally showed lower (<i>p</i> < 0.05) glutathione peroxidase, nitric oxide and catalase activities; and higher superoxide dismutase activity and malondialdehyde levels in the negative control groups. AWO oil could be used in delaying tumor growth, preserving hematological markers and limiting oxidative damage in rats with breast cancer.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"14 5","pages":"e71821"},"PeriodicalIF":3.8,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13129675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147812984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fucoxanthin Ameliorates MASLD by Directly Targeting GRP78 to Restore ER Homeostasis and Activate AMPK Signaling.","authors":"Lin Zhang, Ruifen Zhang, Yu Wu, Binbin Chen, Chao Wang, Cheng Wang, Zhengshun Wen, Jingui Zhang, Haibin Tong, Liang Hong","doi":"10.1002/fsn3.71813","DOIUrl":"https://doi.org/10.1002/fsn3.71813","url":null,"abstract":"<p><p>The identification of direct molecular targets for bioactive dietary components is critical for precision nutrition intervention in metabolic dysfunction-associated steatotic liver disease (MASLD). Fucoxanthin, a marine carotenoid from <i>Sargassum fusiforme</i>, exhibits potent lipid-lowering effects; however, its precise intracellular targets and upstream regulatory mechanisms remain elusive. Herein, using drug affinity responsive target stability (DARTS) coupled with LC-MS/MS, we identified the endoplasmic reticulum (ER) chaperone glucose-regulated protein 78 (GRP78) as a direct binding target of fucoxanthin. Molecular dynamics (MD) simulations and cellular thermal shift assays (CETSA) confirmed a stable interaction, primarily driven by hydrogen bonding at the ARG74 residue. In <i>ob/ob</i> mice and palmitic acid-induced HepG2 cells, fucoxanthin treatment significantly alleviated hepatic steatosis and suppressed ER stress. Mechanistically, the fucoxanthin-GRP78 interaction was found to be indispensable for the subsequent activation of AMP-activated protein kinase (AMPK) signaling. Notably, siRNA-mediated knockdown of GRP78 or pharmacological inhibition of AMPK completely abolished the lipid-lowering and ER stress-relieving effects of fucoxanthin, confirming a causal GRP78-AMPK axis. This study elucidates a novel target-driven mechanism wherein fucoxanthin acts as a GRP78 ligand to restore ER homeostasis and reprogram lipid metabolism. These findings position the fucoxanthin-GRP78 axis as a specific therapeutic target for nutritional strategies against MASLD.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"14 ","pages":"e71813"},"PeriodicalIF":3.8,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13129686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147812847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}