Food Science & Nutrition最新文献

{"title":"Anti-Inflammatory Effects of Hen Egg White Hydrolysate and Its Specific Peptides IS8, PA11, and PK8 on LPS-Induced Macrophage Inflammation","authors":"Ruedeemars Yubolphan,&nbsp;Kajhonpan Phongsiri,&nbsp;Nanthakan Mongkhammee,&nbsp;Jutamas Pidech,&nbsp;Sittiruk Roytrakul,&nbsp;Chonticha Suwattanasophon,&nbsp;Kiattawee Choowongkomon,&nbsp;Sakda Daduang,&nbsp;Watcharee Khunkitti,&nbsp;Nisachon Jangpromma","doi":"10.1002/fsn3.70713","DOIUrl":"https://doi.org/10.1002/fsn3.70713","url":null,"abstract":"<p>Egg white hydrolysate (EWH) has garnered significant attention for the therapeutic properties of their bioactive peptides. This study provides a comprehensive peptidomics analysis of EWH, focusing on its anti-inflammatory potential. Peptides were classified based on their anti-inflammatory potential (AIP) scores to identify promising candidates. In LPS-stimulated RAW 264.7 macrophages, specific peptides effectively suppressed nitric oxide (NO) production, a key inflammatory marker. IS8, PA11, and PK8 were identified as the most promising candidates. qRT-PCR analysis revealed their mechanism of action through modulation of key cytokines, including TNF-α, IL-1β, and IL-10, as well as other mediators such as iNOS and COX-2. Molecular docking studies provided additional insights into the anti-inflammatory potential of these peptides. Notably, IS8, PA11, and PK8 exhibited strong binding affinities to the iNOS active site, with IS8 and PK8 achieving the highest docking scores. The peptides engaged in critical interactions within the iNOS binding pocket through hydrogen bonding and π-π stacking with key residues, suggesting their potential to inhibit iNOS catalytic activity and thereby reduce NO production. These findings highlight the therapeutic potential of EWH-derived peptides, particularly IS8, PA11, and PK8, as anti-inflammatory agents through their ability to modulate key inflammatory pathways, with iNOS inhibition as a central mechanism.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 8","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70713","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144767781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Voluntary Food Safety Disclosure and Government Grants: The Greater Food Approach Perspective","authors":"Wentai Bi,&nbsp;Jie Huan,&nbsp;Peihang Zhang,&nbsp;Xudong Guo,&nbsp;Qi Qi,&nbsp;Yuan Liang","doi":"10.1002/fsn3.70765","DOIUrl":"https://doi.org/10.1002/fsn3.70765","url":null,"abstract":"<p>Voluntary information disclosure is an important bridge between firms and external stakeholders. Based on the data of listed food companies from 2008 to 2022, this study examines the impact of voluntary food safety information disclosure on the government's resource allocation decisions from the perspective of the greater food approach. Such disclosure has a resource-acquisition effect and can significantly increase the level of government grants. The aforementioned effect that is “not what it says on the tin” is only effective in the short term and does not allow for sustained benefits to be realized. The mechanism test established that the positive relationship between disclosure and government grants is significantly inhibited by market attention. Further research finds that this positive association is more pronounced among small-scale and ordinary urban firms, with the types concentrated in revenue-related government grants. This research enriches the literature on the economic consequences of voluntary disclosure and provides empirical evidence for the government to accurately screen firms' food safety status and information manipulation.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 8","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70765","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144767437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eugenol: An Insight Into the Anticancer Perspective and Pharmacological Aspects","authors":"Ahmad Mujtaba Noman,&nbsp;Muhammad Tauseef Sultan,&nbsp;Aimen Mazhar,&nbsp;Waqas Ahmad Khan,&nbsp;Muhammad Imran,&nbsp;Muzzamal Hussain,&nbsp;Ehab M. Mostafa,&nbsp;Ahmed H. El-Ghorab,&nbsp;Mohammed M. Ghoneim,&nbsp;Samy Selim,&nbsp;Mohamed A. Abdelgawad,&nbsp;Entessar Al Jbawi,&nbsp;Suliman A. Alsagaby,&nbsp;Waleed Al Abdulmonem","doi":"10.1002/fsn3.70727","DOIUrl":"https://doi.org/10.1002/fsn3.70727","url":null,"abstract":"<p>The aura of the 21st century has been transformed due to a revolutionized health care system and modern industrial applications. However, human health and well-being are still a global challenge, affected by various metabolic and pathological morbidities, causing millions of mortalities. Cancer is one of the leading causes of fatalities globally, occurring due to pathogens, dietary factors, environmental toxins, occupational chemical exposure, and radiation. These factors lead to oxidative stress (OS), inflammation, DNA damage, epigenetic and genetic mutations, cell proliferation, tumorigenesis, and other chronic metabolic disorders such as diabetes mellitus, cardiovascular disorders, gastrointestinal problems, and hepato-renal syndrome. Among various types of cancers, lung cancer is leading them all, followed by breast and colorectal cancer (CRC). The oncogenesis includes activation of pro-inflammatory markers (IL-6, IL-1β, TNF-α), resulting in overexpression of oncogenes, downregulation of tumor suppressor genes (TSGs) like p53, pRb, APC, PTEN, suppression of antioxidant enzymes (GSH, CAT, SOD), and activation of signaling pathways (PI3K/Akt/mTOR/NF-κB, Ras/MAPK). However, plant-based herbal medicine is a valuable, affordable, and available management approach to reduce the risks of oncogenesis and other ailments. The bioactive compounds of these herbs make them an effective way to reduce inflammation, cancer, and the risk of other health problems. Eugenol (Eug), a volatile phenolic bioactive compound with a formula of C10H12O2, has been reported to have anticancer, antidiabetic, cardio- and pulmonary protective roles. Moreover, it can improve gut health and prevent neurodegenerative disorders. According to WHO, the safe dose of eugenol is 2.5 mg/kg for consumption. The current review focuses on the anticancer potential and other pharmacological aspects of eugenol through possible mechanisms.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 8","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70727","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144767436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotic Enrichment of Jamun Juice With Streptococcus thermophilus: Effects on Antioxidant Activity and Quality","authors":"Dorcus Nnko,&nbsp;Ally Mahadhy","doi":"10.1002/fsn3.70760","DOIUrl":"https://doi.org/10.1002/fsn3.70760","url":null,"abstract":"<p>The increasing demand for functional beverages has driven interest in incorporating probiotics into fruit juices. This study investigates the functionalization of jamun juice with <i>Streptococcus thermophilus</i> (<i>S. thermophilus</i>), a thermophilic probiotic bacterium, to enhance its probiotic properties and assess storage stability under varying temperature conditions. <i>S. thermophilus</i> was isolated from commercial yogurt and identified through colony morphology, Gram staining, and catalase and oxidase tests. The probiotic culture was cultivated in nutrient broth and inoculated into 250 mL of pasteurized jamun juice using 1 mL of bacterial suspension at a concentration of 1.5 × 10⁸ CFU/mL, resulting in a final concentration of 6.0 × 10⁵ CFU/mL in the juice. The functionalized juice was stored at 37°C (incubation), 25°C (room temperature), and 4°C (refrigeration) for 28 days, during which microbial load, pH, sugar content, antioxidant activity, and sensory acceptability were monitored. Results showed a significant increase in microbial load at 37°C, peaking at 10⁹ CFU/mL by Day 14. At room temperature and refrigeration, microbial growth remained minimal, with levels around 10⁸ to 10⁶ CFU/mL. The pH declined most rapidly at 37°C, and at the same temperature, sugar content decreased to 2.2 mg/mL by Day 21. Antioxidant activity, measured using the DPPH assay, was highest (82.35%) at 37°C, indicating enhanced probiotic metabolic activity. Sensory evaluations revealed improved acceptability with lower temperature storage, particularly at refrigeration temperatures, while higher temperatures led to a decrease in sensory quality. Functionalizing jamun juice with <i>S. thermophilus</i> improved its probiotic properties, with temperature playing a key role in maintaining these benefits. Refrigeration preserved both quality and probiotic stability, suggesting the potential of this probiotic jamun juice as a functional beverage. Future research could investigate its long-term stability, sensory characteristics, and nutritional profile, including the presence of bioactive compounds.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 8","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70760","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144767447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Germinated Haricot Bean and Teff-Fortified Barley Porridge for Combatting Pregnant Women's Malnutrition: Optimization Using Mixture Design, Regression Modeling, Contour Plot Analysis, and Nutritional Evaluations","authors":"Ramesh Duraisamy,&nbsp;Abera Terefe,&nbsp;Belay Haile Kebede,&nbsp;Betelhem Abera Mengistu","doi":"10.1002/fsn3.70724","DOIUrl":"https://doi.org/10.1002/fsn3.70724","url":null,"abstract":"<p>In Ethiopia, malnutrition among pregnant women, particularly in rural areas, remains a critical public health challenge, necessitating a strong focus on developing innovative food products. This study aimed to develop optimized composite porridge flours for pregnant women by blending germinated haricot beans, teff, and barley, using regression modeling and contour plot analysis. The flours were blended within 30%–60% for barley, 10%–30% for teff, and 30%–60% for germinated haricot beans. The formulated composite flours exhibited proximate composition values (g/100 g) within the ranges of moisture (8.96–9.46), ash (3.46–4.76), crude fat (2.09–3.64), crude fiber (9.16–10.75), crude protein (14.32–17.57), carbohydrate (66.57–69.58), and energy value (355.57–365.92 kcal/100 g). The mineral content (mg/100 g) included Fe (12.12–16.22), Zn (2.07–5.13), Ca (240.88–582.8), Mg (285.98–534.53), and K (1184.85–1599.99). Anti-nutrient content (mg/100 g) comprised tannins (8.46–13.16) and oxalates (8.77–12.52). The functional properties results (%) encompassed WAC (36.36–56.04) and OAC (35.46–54.65). The sensory evaluations of the porridge showed acceptable scores in appearance, aroma, taste, texture, and overall acceptability. The study revealed that incorporating germinated haricot bean and teff flours into barley flour significantly enhanced its nutritional and functional properties, making it suitable for pregnant women.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 8","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70724","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Profiling, Antioxidant Capacity, and the Antidiabetic Potential of Atriplex halimus Extracts: UHPLC–MS/MS Analysis, In Vitro Enzyme Inhibition Assays, and Molecular Docking Studies","authors":"Mohammed Roubi,&nbsp;Nour Elhouda Daoudi,&nbsp;Mohammed Dalli,&nbsp;Salah-eddine Azizi,&nbsp;Youness Mahdi,&nbsp;Ramzi A. Mothana,&nbsp;Hanan M. Al-Yousef,&nbsp;Mohammed F. Hawwal,&nbsp;Fahd Kandsi,&nbsp;Raffaele Conte,&nbsp;Nadia Gseyra","doi":"10.1002/fsn3.70745","DOIUrl":"https://doi.org/10.1002/fsn3.70745","url":null,"abstract":"<p><i>Atriplex halimus</i> (L.) is known to be characterized by numerous pharmacological prospects comprising antioxidant, anticancer, antibacterial, and antidiabetic properties. The core objective of the present study is to harness the chemical composition of aqueous extract (EA), hydroethanolic extract (EHA), and ethanolic extract (EE), followed by the investigation of the antidiabetic potential of <i>A. halimus</i> extracts on two important targets, porcine proteins (α-amylase and hemoglobin). A molecular docking study was adopted to computationally identify the bioactive compounds responsible for the observed antidiabetic effect. Phytochemical profiling using UHPLC–MS/MS identified 21 compounds across the three extracts, with trans-cinnamic acid being the most abundant in all. The extracts exhibited significant inhibitory effects on both α-amylase and hemoglobin glycation, with IC₅₀ values of 1.89, 3.33, and 2.07 mg/mL (for EA, EHA, and EE, respectively) against α-amylase and 0.29, 0.31, and 0.41 mg/mL against glycation, demonstrating a significant inhibitory impact of <i>A. halimus</i> on both target proteins. With regard to the in silico computational study, it has been demonstrated that both catechin and epigallocatechin emerge as the most active compounds, displaying high binding activity compared to acarbose, a standard antidiabetic drug. These findings highlight the rich phytochemical profile and strong antidiabetic potential of <i>A. halimus</i> extracts, supporting their development as natural therapeutic agents for diabetes management.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 8","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70745","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Thrombotic Effect of Hemp Seed Peptides in Zebrafish by Dual Regulation of vegfr1 Signaling and Caspase-3-Mediated Apoptosis","authors":"Jie Shi,&nbsp;Jingyi Ge,&nbsp;Zhenghai Zhang,&nbsp;Lianhui Wei,&nbsp;Yan Dong,&nbsp;Guowei Li,&nbsp;Qingli Yang,&nbsp;Jing Pan,&nbsp;Yanru Ji","doi":"10.1002/fsn3.70756","DOIUrl":"https://doi.org/10.1002/fsn3.70756","url":null,"abstract":"<p>This study aimed to investigate the antithrombotic activity of hemp seed peptides derived from trypsin hydrolysates and elucidate their molecular mechanisms using a ponatinib-induced zebrafish (<i>Danio rerio</i>) thrombosis model. Through ultrafiltration and LC–MS/MS analysis, we identified 1741 peptide sequences (&lt; 3 kDa), with 67% distributed in the 0.5–1.5 kDa range. In vivo experiments demonstrated that 125 μg/mL hemp seed peptides significantly ameliorated ponatinib-induced thrombosis by increasing 16.55% cardiac erythrocyte staining intensity, enhancing 54.8% blood flow velocity, and restoring 11.2% intersegmental blood vessel diameters. In addition, the observed anticoagulant effect may be partially attributed to upregulated expression levels of vascular endothelial growth factor receptor 1 (<i>vegfr1</i>) and down-regulated expression levels of cysteinyl aspartate-specific proteinase-3 (<i>caspase-3</i>) at the mRNA level. These findings propose hemp seed peptides as novel dietary antithrombotic agents with potential applications in functional food development.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 8","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70756","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Beneficial Effects of Berberine on Brain Functions in Age-Related Neurological Disorders: From Molecular Signaling to Treatment","authors":"Xiaolan Wang,&nbsp;Ruiliang Hou,&nbsp;Zhihao Chen,&nbsp;Xiaoyang Wang,&nbsp;Melika Malek,&nbsp;Haoyu Wang","doi":"10.1002/fsn3.70563","DOIUrl":"https://doi.org/10.1002/fsn3.70563","url":null,"abstract":"<p>Berberine (BBR), a naturally occurring compound with diverse medicinal properties, is emerging as a compelling candidate for managing age-related central nervous system conditions. Preclinical studies have extensively explored its impact on neurodegenerative diseases such as Parkinson's disease, Alzheimer's disease, and Huntington's disease, revealing its capacity to modulate key pathological processes. Mechanistically, BBR is shown to mitigate neuroinflammation, oxidative stress, and endoplasmic reticulum stress, thereby reducing neuronal damage and apoptosis. While these mechanistic insights and preclinical data are robust, clinical evidence remains nascent, necessitating further rigorous investigation to fully understand BBR's therapeutic efficacy and translate its promise into clinical practice. This narrative review provides a comprehensive examination of BBR's application in treating neurological conditions, emphasizing its molecular signaling pathways and critically evaluating the current translational landscape. It also identifies promising directions for future research into BBR's neuroprotective roles.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 8","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70563","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticancer Molecular Mechanisms of Epigallocatechin Gallate: An Updated Review on Clinical Trials","authors":"Ahmad Mujtaba Noman,&nbsp;Muhammad Tauseef Sultan,&nbsp;Aimen Mazhar,&nbsp;Iqra Baig,&nbsp;Jawaria Javaid,&nbsp;Muzzamal Hussain,&nbsp;Muhammad Imran,&nbsp;Suliman A. Alsagaby,&nbsp;Waleed Al Abdulmonem,&nbsp;Ahmed Mujtaba,&nbsp;Tadesse Fenta Yehuala,&nbsp;Mohammed M. Ghoneim,&nbsp;Ehab M. Mostafa,&nbsp;Mohamed A. Abdelgawad","doi":"10.1002/fsn3.70735","DOIUrl":"https://doi.org/10.1002/fsn3.70735","url":null,"abstract":"<p>Human physiology is a complex process encompassing various biochemical mechanisms, regulated by hundreds of chemical mediators, thus making it more complicated to understand. Balance between these mediators is mandatory to continue the helm of life; however, an imbalance can disrupt normal physiological functions and lead to chronic health conditions. Cancer is one such chronic state that occurs due to an imbalance or overproduction of reactive oxygen and nitrogen species (RONS), which are highly unstable and damage other chemical constituents in the body. The mutilation at the cellular level results in DNA and genetic mutation, uncontrolled cell proliferation, metastasis, organ dysfunction, and ultimately mortality. Various types of cancers are intimidating human life globally, and lung cancer is leading them all, followed by breast and colorectal cancer. Oxidative stress (OS) is the main root cause of cancers that occur due to overproduced RONS. Tumor suppressor genes (TSGs) like p53, PTEN, INK4, MADR2, APC, and oncogenes such as HER2, BCR/ABL1, CMYC, EML4AK, RAS, WNT, ERK, and TRK get mutated by OS. Moreover, various pro-inflammatory markers i.e., IL-1, IL-6, TNF-α, IFN-γ, TGF-β and growth factors (VEGF, EGF, IGF-2) are also involved in cancer progression. Along with these aspects, the oncogenesis involves different signaling pathways/axis such as MAPK, PI3K, mTOR, Wnt/β-catenin, GSK3, and NF-κB. Epigallocatechin gallate (EGCG), a bioactive compound abundantly existing in green tea and grapes with strong antioxidant activity and anticancer potential, is a suitable approach to reduce the cancer burden. The in vitro and in vivo anticancer studies of EGCG proved anticancer and anti-tumor effects through modulation of cancer signaling pathways, reduction in cell proliferation, decreased metastasis, suppressed angiogenesis, enhanced antioxidant activity, inhibited pro-inflammatory biomarkers, improved TSGs expression, and downregulated oncogenes expression. Shortly, the current review focuses on the anticancer potential of EGCG through possible mechanisms.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 8","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70735","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144751194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Naringenin Accelerates Diabetic Wound Healing via Regulating Macrophage M2 Polarization and Efferocytosis","authors":"Beizhi Wang,&nbsp;Yumeng Huang,&nbsp;Youjun Ding,&nbsp;Jingyi Chen,&nbsp;Yutong Chen,&nbsp;Hao Zhang,&nbsp;Qian Tan","doi":"10.1002/fsn3.70688","DOIUrl":"https://doi.org/10.1002/fsn3.70688","url":null,"abstract":"<p>Diabetic wounds that do not heal are a significant complication of diabetes, among which chronic inflammation serves as a critical contributing factor. The regulation of inflammation in diabetic wound healing is significantly impacted by macrophages via their efferocytosis and polarization activities. Naringenin is a natural flavonoid that has been recognized for its anti-inflammatory effects and potential therapeutic benefits in treating diabetes-related conditions. This research examines how naringenin impacts non-healing diabetic wounds and delves into the mechanisms behind it. The study involved male C57BL/6 mice categorized into three groups: control, diabetes, and diabetes with naringenin treatment. Researchers assessed the effect of naringenin on wound healing by applying it topically to a diabetic mouse model induced with streptozotocin (STZ). Moreover, researchers used THP-1 cells in vitro to examine the effects of naringenin on the polarization of macrophages to the M2 phenotype and their efferocytosis. Subsequently, the effects of naringenin treatment were assessed using RT-qPCR, western blot, immunofluorescence, and additional assays. Animal experiments demonstrated that naringenin significantly accelerated diabetic wound healing. Naringenin decreased inflammatory cytokines, promoted M2 macrophage polarization, and enhanced macrophage efferocytosis in wound healing. Consistent with animal studies, naringenin inhibits macrophage M1 polarization while augmenting M2 polarization in THP-1 cells, with ML385 specifically rescuing the M1 suppression. In addition, naringenin also promoted macrophage efferocytosis in a THP-1/Jurkat apoptotic co-culture mode. Naringenin significantly promotes diabetic wound healing via ameliorating wound inflammation and exerts its therapeutic effects through promoting M2 macrophages and enhancing efferocytosis.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 8","pages":""},"PeriodicalIF":3.8,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70688","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144751195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}