Food Science & Nutrition最新文献

{"title":"Effects of Different Dietary Levels of Chia (<i>Salvia hispanica</i> L.) and Quinoa (<i>Chenopodium quinoa</i> Willd.) on Adipokines and Anthropometric Parameters in Rats.","authors":"Kübra İspir, Sevinç Yücecan, Ilyas Bozkurt, Halit Imik","doi":"10.1002/fsn3.72325","DOIUrl":"https://doi.org/10.1002/fsn3.72325","url":null,"abstract":"<p><p>Chia (<i>Salvia hispanica</i> L.) and quinoa (<i>Chenopodium quinoa</i> Willd.) are pseudocereals that stand out due to their high nutrient density and functional component content. Their richness in protein, dietary fiber, unsaturated fatty acids, vitamins, minerals, and bioactive phytochemicals makes them important dietary components with potential roles in metabolic processes and inflammation-related mechanisms. This study aimed to evaluate the effects of chia and quinoa seeds, included at 5%, 10%, and 20% in isocaloric and isonitrogenous diets, on serum adipokines (omentin, visfatin, resistin, chemerin), insulin, anthropometric measurements, and body mass index (BMI) in healthy, normal weight male Wistar rats, relative to a control diet. Seventy male Wistar albino rats were divided into seven groups: control diet, 5%, 10%, and 20% chia seed diets (LD-CD, MD-CD, HD-CD) and 5%, 10%, and 20% quinoa seed diets (LD-QD, MD-QD, HD-QD). After 6 weeks, serum adipokine and insulin levels were measured. Weekly body weight, body length, abdominal circumference, and chest circumference were recorded, and BMI values were calculated. Chia and quinoa supplementation altered serum adipokine and insulin levels, with the observed responses varying according to the supplementation level. High-dose (20%) supplementation decreased serum chemerin, resistin, and insulin levels while increasing omentin levels compared with controls. Moderate (10%) and high (20%) supplementation altered serum visfatin levels. Chia and quinoa supplemented diets reduced BMI compared with controls. The HD-CD group also showed lower body weight gain and abdominal circumference. Including chia and quinoa seeds as dietary alternatives may positively affect serum adipokine levels and anthropometric parameters.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"14 9","pages":"e72325"},"PeriodicalIF":5.0,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148896791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epigallocatechin Gallate From Tea Delays Oocyte Aging After Ovulation in Mice.","authors":"Jing Lin, Hua-Chao Gao, Li-Jun Li, Miao Hu, Li-Fan Guo, Hong-Yan Guo, Xiao-Wen Jin, Peng Xu, Zhao-Jia Ge, Lei Zhao","doi":"10.1002/fsn3.72324","DOIUrl":"https://doi.org/10.1002/fsn3.72324","url":null,"abstract":"<p><p>In clinic, once the ovulated oocytes cannot be fertilized timely, they begin to undergo post-ovulatory aging, which impacts the embryo development and offspring health. Until now, there are no better methods to delay post-ovulatory oocyte aging. Epigallocatechin gallate (EGCG) is the most abundant bioactive component of tea polyphenols that are beneficial for alleviating the deleterious influence of environmental factors on oocyte quality. In the present study, we examined the role of EGCG in delaying oocyte aging after ovulation in vitro. Ovulated oocytes were treated with EGCG at different concentrations, and the fragmentation rate, induced by aging, was significantly reduced by EGCG at 50 μM in vitro. To further elucidate the influence of EGCG on the quality of post-ovulatory aged oocytes, we examined the spindle morphology of aged oocytes. The results showed that EGCG significantly decreased the abnormal rate of spindle morphology in post-ovulatory aged oocytes. The high level of reactive oxygen species (ROS) and mitochondrial dysfunction in post-ovulatory aged oocytes were also improved by EGCG, which might be a reason for the reduced apoptosis. The reduced sperm binding capacity of aged oocytes was increased by the addition of EGCG. These suggest that EGCG can improve the quality of post-ovulatory aged oocytes.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"14 9","pages":"e72324"},"PeriodicalIF":5.0,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148896812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carboxymethylated <i>Poria cocos</i> Polysaccharides Mitigate Cisplatin-Induced Hepatorenal Injury by Activating the PLC-PKC-Nrf2 Signaling Pathway.","authors":"Liang He, Shuo Chen, Baoming Wu, Jiong Gu, Conghan Li, Xianfan Du, Jun Chu, Min Yang, Hui Hou","doi":"10.1002/fsn3.72342","DOIUrl":"https://doi.org/10.1002/fsn3.72342","url":null,"abstract":"<p><p>Cisplatin, a commonly used chemotherapy drug, inevitably causes damage to the liver and kidneys while exerting its anti-tumor effects. Carboxymethylated <i>Poria cocos</i> polysaccharides (CMP) have anti-inflammatory, antioxidant, and organ-protective properties, and also exhibit significant anti-tumor activity. Therefore, this study aims to investigate the effects of CMP on cisplatin-induced hepatorenal damage in mice. The results showed that CMP treatment significantly mitigated abnormalities in hepatorenal function indicators and tissue damage induced by cisplatin. Furthermore, CMP significantly suppressed the increase in levels of inflammatory factors in peripheral and hepatorenal tissues, as well as the infiltration of immune cells induced by cisplatin. Additionally, CMP significantly enhanced the antioxidant capacity of hepatorenal tissues and inhibited oxidative damage caused by cisplatin. In vitro, CMP have also been shown to protect renal tubular epithelial cells and hepatocytes from cisplatin-induced damage. Mechanistically, our studies indicated that CMP activate Nrf2 through a phosphorylation pathway that is independent of Keap1 and reliant on PLC-PKC signaling. In conclusion, our findings suggest that CMP has the potential to serve as an adjunctive agent in cisplatin chemotherapy to protect the kidneys and liver from damage.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"14 9","pages":"e72342"},"PeriodicalIF":5.0,"publicationDate":"2026-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545543/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148896809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Lactiplantibacillus plantarum</i> BF_15 Confers Protection Against Ulcerative Colitis by Orchestrating Gut Microbiota-Barrier-Immune Triad Homeostasis.","authors":"Na Zhang, Hongjie Liu, Dongyao Li, Chen Li, Xinyi Ge, Hongyan Kang, Miaoshu Wang, Hongtao Tian, Xiaoyan Miao","doi":"10.1002/fsn3.72275","DOIUrl":"https://doi.org/10.1002/fsn3.72275","url":null,"abstract":"<p><p>Ulcerative colitis (UC) is a prevalent chronic inflammatory bowel disease globally, driven mainly by immune dysregulation and gut microbiota dysbiosis. This study focused on <i>Lactiplantibacillus plantarum</i> BF_15, an infant-derived strain with potential to alleviate UC. Four experimental groups were established, including the control group, DSS group, BF_15 group, and LGG group. Probiotics were administrated at a dosage of 1 × 10⁹ CFU/mL and 0.1 mL/10 g body weight (BW) for 21 days. The therapeutic effects and mechanisms of BF_15 against UC were explored based on physiological indicators, gut microbiota, and tandem mass tag (TMT)-based quantitative proteomics. The results showed that BF_15 intervention significantly reduced the disease activity index (DAI) from 3.00 ± 0.50 to 1.16 ± 0.36 (<i>p</i> < 0.05) and mitigated UC-related symptoms including body weight loss and colon shortening, with colon length restored from 4.43 ± 0.32 cm to 5.41 ± 0.26 cm (<i>p</i> < 0.05). Meanwhile, BF_15 downregulated the levels of pro-inflammatory factors such as interleukin IL-6 and IL-17A, and upregulated the levels of IL-10 (from 565.92 ± 79.40 pg/mL to 656.84 ± 66.01 pg/mL) and secretory immunoglobulin A (SIgA, from 9.75 ± 1.29 μg/mL to 12.79 ± 1.77 μg/mL) (<i>p</i> < 0.05), with a therapeutic effect comparable to that of LGG. Further, gut microbiota analysis demonstrated that BF_15 restored the relative abundance of <i>Bacteroidetes</i> (from 28.02% to 30.85%) and regulated gut microbiota homeostasis. Finally, TMT-based proteomic analysis indicated that BF_15 regulated 528 differentially expressed proteins (DEPs) and exhibited a superior regulatory effect on the antigen presentation and processing (APC) pathway compared with LGG. The above findings provide potential novel therapeutic targets and probiotic strain resources for the treatment of UC.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"14 9","pages":"e72275"},"PeriodicalIF":5.0,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545421/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148896798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Inoculation Strategy on <i>Dendrobium nobile</i> Lindl. Rice Wine Quality Through the Use of <i>Saccharomyces cerevisiae</i> FBKL2.8022 and <i>Wickerhamomyces anomalus</i> FBKL2.8023.","authors":"Hongling Gan, Guichun Huang, Lin Zhang, Lanyan Cen, Yifeng Dai, Shuyi Qiu, Xiaodan Wang, Chaoyang Wei","doi":"10.1002/fsn3.72321","DOIUrl":"https://doi.org/10.1002/fsn3.72321","url":null,"abstract":"<p><p>This study examined how the inoculation sequence of <i>Saccharomyces cerevisiae</i> (<i>Sc</i>) and <i>Wickerhamomyces anomalus</i> (<i>Wa</i>), along with <i>Dendrobium nobile</i> Lindl. (<i>D. nobile</i>) addition, affects rice wine quality. Sequential inoculation with <i>D. nobile</i> yielded the highest levels of polysaccharides, alkaloids, flavonoids, and phenols. While simultaneous inoculation raised alcohol proportion, sequential inoculation increased ester content. <i>D. nobile</i> reduced total flavor concentration but enhanced its diversity. Metabolic analysis indicated that both factors alter metabolites mainly via amino acid and sugar pathways. Thus, sequential <i>Sc</i>-<i>Wa</i> inoculation with <i>D. nobile</i> is an effective strategy for modulating wine composition, providing a reference for improving fermented rice wine quality.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"14 9","pages":"e72321"},"PeriodicalIF":5.0,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148896922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sodium Intake and Osteoporosis Risk: Evidence From NHANES and Mendelian Randomization Analysis.","authors":"Yunxiao Ji, Zibo Fan, Rugang Zhao, Changsong Zhao, Qiang Zhang, Xinbao Wu","doi":"10.1002/fsn3.72147","DOIUrl":"https://doi.org/10.1002/fsn3.72147","url":null,"abstract":"<p><p>Osteoporosis remains a critical public health challenge worldwide, particularly for older adults. Dietary sodium intake is considered a potentially modifiable factor related to bone mineral density (BMD) and osteoporosis risk, but existing findings remain inconsistent, and large-scale evidence is limited. This study combines cross-sectional analysis from the National Health and Nutrition Examination Survey (NHANES) with Mendelian randomization (MR) to investigate the association and potential causal relationship between sodium intake and osteoporosis. Drawing on NHANES data from the 2005-2010, 2013-2014, and 2017-2018 cycles (<i>n</i> = 3350), we applied weighted multivariable logistic regression to evaluate the association between sodium intake and osteoporosis risk. Leveraging sodium metabolism-related single-nucleotide polymorphisms (SNPs) as instrumental variables, we performed two-sample Mendelian randomization (MR) analysis. MR estimates were derived using inverse-variance weighting (IVW), MR-Egger regression, and weighted median methods, with robustness assessed using pleiotropy tests and leave-one-out sensitivity analysis. Cross-sectional analysis showed that each 1 mg/day increase in two-day average dietary sodium intake was associated with approximately 0.011% lower odds of osteoporosis (OR = 0.99989, 95% CI: 0.99979-1.0000). Compared with the lowest quartile (Q1), participants in Q3 (OR = 0.48, 95% CI: 0.30-0.78, <i>p</i> = 0.004) and Q4 (OR = 0.51, 95% CI: 0.28-0.93, <i>p</i> = 0.028) had lower odds of osteoporosis (<i>p</i>-trend < 0.05). MR analysis using the IVW method provided genetic evidence supporting a potential inverse association (OR = 0.51, 95% CI: 0.28-0.95), although the MR-Egger estimate was nonsignificant and had a wide confidence interval (<i>p</i> = 0.312). These findings support a potential inverse association between sodium intake and osteoporosis risk in this study population. Further prospective and experimental studies are needed to validate these findings, clarify the underlying mechanisms, and determine whether they have any implications for dietary recommendations.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"14 9","pages":"e72147"},"PeriodicalIF":5.0,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13543167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148896899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Physicochemical Properties of Chitosan-Gum Arabic Nanoparticles Containing <i>Ziziphora clinopodioides</i> Lam. Extract Using Response Surface Methodology.","authors":"Anita Ehterami, Hassan Gandomi, Mohammad Kazem Koohi, Negin Noori, Masoud Kazeminia","doi":"10.1002/fsn3.72318","DOIUrl":"https://doi.org/10.1002/fsn3.72318","url":null,"abstract":"<p><p><i>Ziziphora clinopodioides</i> Lam. extract, as a natural bioactive compound, possesses a wide range of health benefits; however, its inherent instability limits practical applications. To overcome this limitation, nanoparticle-based delivery systems, particularly chitosan-gum arabic nanoparticles, were employed. The aim of this study was to evaluate the effects of varying concentrations of chitosan (3-9 mg/mL), <i>Z. clinopodioides</i> L. extract (3-9 mg/mL), and gum arabic (0.5-1.5 mg/mL), on the physicochemical characteristics of chitosan-gum arabic nanoparticles containing the extract, using a two-step emulsion-ionic gelation approach. Key parameters, including particle size, zeta potential, and encapsulation efficiency, were assessed by examining both individual and interactive effects. The process was optimized using response surface methodology (RSM) with a Box-Behnken design. The FTIR and XRD analyses were conducted to characterize the nanoparticles. Nanoparticle sizes ranged from 13.90 to 1722.75 nm, while the zeta potentials ranged from 1.63 to 46.67 mV, and the encapsulation efficiencies ranged from 20.51% to 63.66%. Notably, increasing the extract concentration reduced the encapsulation efficiency, while increasing particle size and zeta potential. Higher chitosan concentrations increased nanoparticle zeta potential. In contrast, increasing gum arabic concentration enhanced the encapsulation efficiency. The results demonstrated significant interaction effects among the formulation parameters across all measured responses. Optimal concentrations of chitosan, extract, and gum arabic were determined to be 9, 4.90, and 1.5 mg/mL, respectively, yielding a particle size of 50 nm, a zeta potential of 5.86 mV, and an encapsulation efficiency of 60.63%. The FTIR and XRD analyses confirmed proper encapsulation of the extract. These findings highlight the critical effects of studied parameters on practical characteristics of chitosan-gum arabic nanoparticles loaded with <i>Z. clinopodioides</i> L. extract.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"14 9","pages":"e72318"},"PeriodicalIF":5.0,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13545426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148896905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of <i>CYP2E1</i> Polymorphism and Dietary Supplementation With Jerusalem Artichoke on Skatole and Indole Levels in the Back Fat and Feces of Boars, and Liver mRNA Expression.","authors":"Kateřina Zadinová, Antonín Stratil, Jaroslav Čítek, Monika Okrouhlá, Jan Calta, Ivan Bahelka, Kateřina Urbancová, Michal Šprysl, Roman Stupka","doi":"10.1002/fsn3.72272","DOIUrl":"https://doi.org/10.1002/fsn3.72272","url":null,"abstract":"<p><p>The objective of this study was to evaluate the effects of adding Jerusalem artichoke to the diet of fattening boars and the effects of polymorphisms in the <i>CYP2E1</i> gene on skatole levels in back fat and feces and on <i>CYP2E1</i> mRNA expression in pig liver. A total of 25 crossbreed boars (LW_S × Pn) × (L_D × LW_D) were used in this study. All boars were genotyped for c.1423G > A. The pigs were divided into two different dietary treatment groups with 0% (C-control; <i>n</i> = 12) and 8.2% (E-experimental; <i>n</i> = 13) dried Jerusalem artichoke tubers, which were fed 14 days before slaughter. The results suggest that feeding Jerusalem artichoke decreased (<i>p</i> = 0.0236) the level of skatole in adipose tissue. In feces, a decrease in the skatole level was observed, but there was no statistically significant difference between the control and experimental groups. The influence of polymorphism on skatole levels in adipose tissue and feces were lowest for the <i>AA</i> genotype and highest for the <i>GG</i> genotype. Differences between genotypes for skatole levels in feces were statistically significant (<i>p</i> = 0.0242). Differences between genotypes in adipose tissue on skatole levels showed the same trend in favor of the <i>A</i> allele. However, these differences were not statistically significant. This study also confirmed the significant effect of Jerusalem artichoke on the liver <i>CYP2E1</i> mRNA expression. The <i>CYP2E1</i> gene expression level was significantly higher in the experimental group (<i>p</i> = 0.0086). No significant differences in mRNA expression between different genotypes were detected.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"14 9","pages":"e72272"},"PeriodicalIF":5.0,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13542426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148896744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anthocyanin Fluorescence for Monitoring Oxidation of the Chokeberry Fruit During Drying and Storage of Fruit Lyophilizates.","authors":"Małgorzata Rak, Grzegorz Bartosz, Kacper Kut, Ireneusz Kapusta, Maria Goc, Izabela Sadowska-Bartosz","doi":"10.1002/fsn3.72223","DOIUrl":"10.1002/fsn3.72223","url":null,"abstract":"<p><p>The appearance of the \"oxidation band\" in the fluorescence spectrum of anthocyanins is an indicator of oxidative deterioration of anthocyanins and can be a useful preliminary index of food freshness. Chokeberry (<i>Aronia melanocarpa</i>) fruit, rich in anthocyanins, can be subject to drying or lyophilization for domestic or industrial applications. The study was aimed at determining whether the measurement of fluorescence of dried chokeberry extracts can provide information on the extent of anthocyanin oxidation during prolonged drying at 60°C, and if fluorescence of fruit extracts can monitor anthocyanin oxidation during storage of the lyophilizates at various temperatures under air exposure. The increase in the fluorescence intensity of the extracts of chokeberry fruits or their lyophilizates at the \"oxidation band\" was a regular function of the time of fruit drying and the time of lyophilizate storage at 22°C and 37°C, confirming the usefulness of this parameter for estimation of oxidative changes in dried fruits and their lyophilizates. However, fluorescence of whole fruits, pulverized fruits and pulverized lyophilizate did not show a regular increase as a function of the time of fruit drying and lyophilizate storage, apparently due mainly to the effects of light scattering. The results point to the necessity of controlling the drying process of chokeberry fruits and storing their lyophilizates at ambient or higher temperatures with air access to avoid anthocyanin oxidation.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"14 9","pages":"e72223"},"PeriodicalIF":5.0,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13539620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148886587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimisation of Microwave-Assisted Extraction and Spray Drying for Chlorophyll Powder Production From <i>Ceratophyllum submersum</i> L.","authors":"Hoang Thi Ngoc Nhon, Lam Nguyen Dan Khoa, Le Thi Hong Anh","doi":"10.1002/fsn3.72320","DOIUrl":"10.1002/fsn3.72320","url":null,"abstract":"<p><p>This study aimed to optimize chlorophyll extraction from <i>Ceratophyllum submersum</i> L. and develop a stable spray-dried chlorophyll powder. Microwave-assisted extraction was optimized using Response Surface Methodology (Box-Behnken design) with MgCO₃ concentration, microwave power, and extraction time as variables. The optimal conditions (0.763% MgCO₃, 372.1 W, 5.28 min) yielded 0.187 mg/g dry mass, which closely matched the predicted value. Spray drying parameters were optimized at 25% maltodextrin, a feed rate of 6 mL/min, and an inlet temperature of 160°C. SEM revealed spherical particles with typical surface wrinkling; FTIR showed spectral features consistent with the chlorophyll-maltodextrin matrix, without allowing specific differentiation between chlorophyll and its degradation products, while XRD revealed a predominantly amorphous structure. The resulting powder exhibited a low moisture content (3.25%), high solubility (83.72%), and acceptable flowability, with a Carr index of 23.45% and a Hausner ratio of 1.30. The measured microbiological, heavy-metal, and residual-solvent levels met the evaluated safety criteria. These findings demonstrate the potential of <i>C. submersum</i> as an underutilized source of natural chlorophyll and provide laboratory-scale processing conditions for further compositional, storage-stability, application, and scale-up studies.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"14 9","pages":"e72320"},"PeriodicalIF":5.0,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13539616/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148886709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}