{"title":"Assisted Fermentation by a Modified <i>Bacillus subtilis</i> Strain Producing Protease Improved the Quality of Sufu.","authors":"Junfei Xu, Aixiang Hou, Wenqi Li, Binbin Chen, Hong Wu, Huan Tan, Zhihong Xiao, Xianjin Wu, Juzuo Zhang","doi":"10.1002/fsn3.4673","DOIUrl":"https://doi.org/10.1002/fsn3.4673","url":null,"abstract":"<p><p>Traditionally fermented sufu is popular because of its flavor, abundance of nutrients, and long shelf life. However, traditional sufu is difficult to produce via industrial processes because of dominant microorganism attenuation during fermentation. Herein, specific protease-producing strains were isolated from traditional sufu. After strain identification, mutation, and domestication, the strains were applied in fermentation. The taste, texture, and nutrient and flavor components of the fermentation products were investigated via organoleptic, textural HPLC and HS-GC-IMS analyses. Results revealed that <i>Bacillus subtilis</i> (DF1) and the derived strains DF1v and DF1vd had increased protease activity relative to other strains. When these strains were applied for sufu fermentation, the production period significantly shortened to 6-8 days for pehtzes and to 20-26 days for postripening. The nutrient and flavor compound composition of both sufu pehtzes and products improved, including increases in water-soluble proteins, amino acids, and substances with beany and umami aromas and decreases in nonbeneficial biogenic amines and moldy odor-imparting substances. Among the strains, DF1vd showed the greatest benefits in sufu-assisted fermentation. In summary, a modified <i>Bacillus subtilis</i> strain (DF1vd) producing protease was isolated, which improved the nutrient profile and flavor of sufu and shortened the production period.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 1","pages":"e4673"},"PeriodicalIF":3.5,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11770482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Seda Arioglu-Tuncil, Dane Deemer, Stephen R Lindemann, Yunus E Tunçil
{"title":"Coconut (<i>Cocos nucifera</i> L.) and Carob (<i>Ceratonia siliqua</i> L.) Flours Dietary Fibers Differentially Impact Fecal Microbiota Composition and Metabolic Outputs In Vitro.","authors":"Seda Arioglu-Tuncil, Dane Deemer, Stephen R Lindemann, Yunus E Tunçil","doi":"10.1002/fsn3.4724","DOIUrl":"10.1002/fsn3.4724","url":null,"abstract":"<p><p>Alternative flours can reveal beneficial health effects. The aim of this study was to evaluate and compare the effects of dietary fibers (DFs) of coconut and carob flours on colonic microbiota compositions and function. Coconut flour DFs were found to be dominated by mannose-containing polysaccharides by gas chromatography (GC)/MS and spectrophotometer, whereas glucose and uronic acid were the main monosaccharide moieties in carob flour DFs. In vitro fecal fermentation analysis revealed that coconut flour DFs result in the generation of microbial butyrate as much as inulin does, which is known to be a butyrogenic prebiotic, but at a slower rate. Supportingly, coconut flour DFs promoted butyrate-producing bacteria including <i>Roseburia</i> and <i>Coprococcus</i>, whereas carob flour DFs stimulated <i>Prevotella</i>-related OTUs. In addition, higher microbial diversity was achieved at the end of the fermentation of coconut flour DFs by the fecal microbiota. This study clearly shows that alternative flours have distinct functionalities in terms of colonic microbiota composition and function, and coconut flour could be used as an alternative flour for the development of functional food products targeting colonic health.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 1","pages":"e4724"},"PeriodicalIF":3.5,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762452/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Da Su, Zhiya Jin, Jie Ou, Muhammad Atif Muneer, Yunfei Jiang, Delian Ye, Liangquan Wu, Xiaojun Yan
{"title":"Spatial-Temporal Variation of Grain Magnesium, Calcium and Their Response to Phosphorus Nutrition in Sweet Corn.","authors":"Da Su, Zhiya Jin, Jie Ou, Muhammad Atif Muneer, Yunfei Jiang, Delian Ye, Liangquan Wu, Xiaojun Yan","doi":"10.1002/fsn3.4725","DOIUrl":"10.1002/fsn3.4725","url":null,"abstract":"<p><p>Sweet corn (<i>Zea mays</i> L. <i>saccharata</i>) is gaining global popularity as a staple crop and a vegetable due to its high nutritional value. However, information on grain magnesium (Mg) and calcium (Ca) status and their response to phosphorus (P) fertilization in sweet corn is still insufficient. In the current study, we combined the genotype evaluation and P gradient experiments to investigate how P supply influences the spatio-temporal variation of grain mineral nutrition (Mg and Ca) and their bioavailabilities in sweet corn. Increasing P decreased grain Ca by 16.7%, from 0.18 to 0.15 g kg<sup>-1</sup>, but increased phytic acid phosphorus (PAP) concentration by 7.4%. Optimal P levels increased grain Mg concentration by 14.7%, from 1.36 to a peak of 1.56 g kg<sup>-1</sup>; however, excessive P reduced it by 3.8% to 1.50 g kg<sup>-1</sup>. Spatially, optimal P increased the grain Mg concentration across all cob positions and in the germ, with the largest variation observed at the upside position (Coefficient of variation (cv) = 11.88%). Conversely, high P decreased Mg concentration in middle-cob grains and the germ. High P also reduced Ca in the upper/middle-cob grain and in both germ and endosperm. P consistently increased grain PAP but reduced Mg and Ca bioavailability across all growth stages, all grain fractions, and the middle/bottom cob positions. Notably, PAP showed the largest variation at the late growth stage in bottom cob grains (CV = 8.75%). Mg and Ca bioavailabilities exhibited significant variations, primarily in upper grains during the early filling stage, with CVs reaching 14.5% and 43.23%, respectively. Temporally, early grain growth stage was more sensitive to P-induced changes in Mg, Ca and their bioavailabilities, while later growth stage was more responsive to grain PAP alterations. These findings suggest that reducing P input can be advantageous for achieving high Mg and Ca biofortified sweet corn grain.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 1","pages":"e4725"},"PeriodicalIF":3.5,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762451/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Saurabh Bhatia, Muhammad Jawad, Sampath Chinnam, Ahmed Al-Harrasi, Yasir Abbas Shah, Talha Shireen Khan, Mohammed Said Al-Azri, Esra Koca, Levent Yurdaer Aydemir, Sevgin Dıblan, Syam Mohan, Asim Najmi, Asaad Khalid, Mahbubur Rahman Khan
{"title":"Development and Characterization of Potato Starch-Pectin-Based Active Films Enriched With Juniper Berry Essential Oil for Food Packaging Applications.","authors":"Saurabh Bhatia, Muhammad Jawad, Sampath Chinnam, Ahmed Al-Harrasi, Yasir Abbas Shah, Talha Shireen Khan, Mohammed Said Al-Azri, Esra Koca, Levent Yurdaer Aydemir, Sevgin Dıblan, Syam Mohan, Asim Najmi, Asaad Khalid, Mahbubur Rahman Khan","doi":"10.1002/fsn3.4688","DOIUrl":"10.1002/fsn3.4688","url":null,"abstract":"<p><p>The increasing demand for sustainable food packaging has driven the development of films based on biopolymers. However, enhancing their functional properties remains a challenge. In the current study, potato starch-pectin (PSP) composite films were fabricated and enriched with juniper berry essential oil (JBEO) to improve their physicochemical properties. The effects of incorporating different concentrations of JBEO (0.1%-1% v/v) on various properties of PSP-based films were evaluated, including surface color, transparency, barrier properties, scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermal analysis (TGA and DTA), antioxidant activity, and antimicrobial effectiveness. Increasing the level of JBEO led to a significant decrease in the moisture content, film transparency, and mechanical attributes, while an increase in thickness, water permeability, and film elongation was observed. SEM analysis also revealed morphological properties such as some spherical, bubble-like configuration and cracks on the surface due to an increase in JBEO concentration. TGA and DTA revealed lower weight loss in the initial cycles due to the addition of JBEO, and the thermal stability of the films improved. The antioxidant assays revealed a concentration-dependent increase in the radical scavenging capacity of the films from 11.31% to 17.28% for DPPH and from 3.06% to 25.53% for ABTS. Moreover, significant antibacterial and antifungal activity of the bioactive films was observed against <i>P. aeruginosa</i>, <i>S. aureus</i>, and <i>C. albicans</i>. These findings suggest that JBEO enhances the functional properties of PSP films, making them suitable for active food packaging applications.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 1","pages":"e4688"},"PeriodicalIF":3.5,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11761362/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Manzeremu Rejiepu, Jun Shen, Junqing Liang, Alaili Maitikabili, Jian Yang, Ling Zhang, Na Mi
{"title":"<i>Peganum harmala</i> Polysaccharide Mitigates LPS-Induced Inflammatory Response in Macrophages by Activating Autophagy Pathway.","authors":"Manzeremu Rejiepu, Jun Shen, Junqing Liang, Alaili Maitikabili, Jian Yang, Ling Zhang, Na Mi","doi":"10.1002/fsn3.4501","DOIUrl":"10.1002/fsn3.4501","url":null,"abstract":"<p><p><i>Peganum harmala,</i> a member of the <i>Zygophyllaceae</i> family, is known for its diverse biological activities, including anti-inflammatory properties. The mechanisms through which <i>P. harmala</i> polysaccharide (LTP) induces autophagy, however, remain largely unexplored. This study aims to elucidate the role of LTP in autophagy induction and its efficacy in mitigating inflammation within macrophages. Autophagosome formation was evaluated using GFP-LC3 vectors, and LC3-II levels induced by LTP were analyzed through laser scanning confocal microscopy. Western blotting assessed the expression of autophagy-related proteins and the phosphorylation state of p70S6K in NRK cells, treated both with and without LTP, alongside autophagy inducers and inhibitors. Additionally, RAW264.7 cells were treated with 1 μg/mL lipopolysaccharides (LPS), followed by Western blotting and ELISA assays to quantify inflammatory markers. The study's outcomes demonstrate that LTP facilitates an increase in autophagic activity, as evidenced by the enhanced expression of LC3-II and reduced levels of p62 in both NRK and RAW264.7 macrophages. This effect is mediated through the activation of the AMPK-mTOR signaling pathway, without inhibiting the autophagosome-lysosome fusion process. In vitro experiments with RAW264.7 cells treated with 1 μg/mL LPS showed that LTP markedly decreased the levels of TNF-α and IL-6. Our findings indicate that LTP effectively reduces inflammation in LPS-stimulated macrophages by promoting autophagy via an mTOR-dependent mechanism.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 1","pages":"e4501"},"PeriodicalIF":3.5,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11761410/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chengyu Lv, Song Wang, Chang Sun, Jing Liu, Yihao Chen, Chao Wang, Cuiping Yuan, Fengxian Qin, Tiezhu Li
{"title":"Psoralen and Isopsoralen Activate Nuclear Factor Erythroid 2-Related Factor 2 Through Interaction With Kelch-Like ECH-Associated Protein 1.","authors":"Chengyu Lv, Song Wang, Chang Sun, Jing Liu, Yihao Chen, Chao Wang, Cuiping Yuan, Fengxian Qin, Tiezhu Li","doi":"10.1002/fsn3.4768","DOIUrl":"10.1002/fsn3.4768","url":null,"abstract":"<p><p>As natural furocoumarins, psoralen and its isomer isopsoralen are widely distributed in various fruits including <i>Ficus carica</i> L., vegetables including celery, and medicinal herbs including <i>Psoralea corylifolia</i> L. Although psoralen and isopsoralen have been used as dietary supplements because of their bioactivities such as antibacterial and anti-inflammatory properties; however, the potential mechanisms underlying the antioxidant activities of these two furocoumarins still need to be explored. Hence, the aims of this work were to examine the activation of nuclear factor erythroid 2-related factor 2 (Nrf2) by psoralen and isopsoralen, as well as the binding interaction of Kelch-like ECH-associated protein 1 (Keap1) with these two furocoumarins. Interestingly, both psoralen and isopsoralen induced Nrf2 nuclear translocation in a dose-dependent manner in HEK293T cells. These two furanocoumarins also activated antioxidant response element (ARE)-driven luciferase activity. The mRNA expression of GCLM, HO-1, and NQO1 genes was significantly upregulated by treatment of HEK293T cells with psoralen and isopsoralen, respectively. Similarly, the expression of proteins can be promoted. Both psoralen and isopsoralen were located in the top of the central pocket of the Keap1 Kelch domain, suggesting that they were natural ligands of Keap1. In conclusion, both psoralen and isopsoralen activate Nrf2 through interaction with Keap1, thereby serving as natural antioxidants.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 1","pages":"e4768"},"PeriodicalIF":3.5,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11761412/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Food Science & NutritionPub Date : 2025-01-22eCollection Date: 2025-01-01DOI: 10.1002/fsn3.70003
Heyan Xu, Yuna Wang, Dandan Geng, Fengming Chen, Yujia Chen, Lisa Cynthia Niwenahisemo, Lei Shi, Ning Du, Ziqiang He, Xiaoming Xu, Li Kuang
{"title":"Lycopene Alleviates Depression-Like Behavior in Chronic Social Defeat Stress-Induced Mice by Promoting Synaptic Plasticity via the BDNF-TrkB Pathway.","authors":"Heyan Xu, Yuna Wang, Dandan Geng, Fengming Chen, Yujia Chen, Lisa Cynthia Niwenahisemo, Lei Shi, Ning Du, Ziqiang He, Xiaoming Xu, Li Kuang","doi":"10.1002/fsn3.70003","DOIUrl":"10.1002/fsn3.70003","url":null,"abstract":"<p><p>Lycopene is a natural plant extract widely studied for its powerful antioxidant and neuroprotective effects. Emerging evidence suggests that it also possesses potential antidepressant properties. Compared to commonly used clinical antidepressants, lycopene offers higher safety; however, its underlying mechanisms remain unclear. Therefore, this study aims to explore the mechanisms through which lycopene exerts its antidepressant effects. We employed the chronic social defeat stress (CSDS) model to induce depressive-like behaviors in mice, followed by lycopene treatment (20 mg/kg). Based on previous research, we focused on synaptic plasticity by examining the expression of synaptic proteins in the hippocampus to uncover potential mechanisms. The results showed that CSDS induced synaptic plasticity impairments in the hippocampus but lycopene treatment significantly improved these synaptic deficits and reversed the depressive-like behaviors induced by CSDS. Moreover, lycopene treatment upregulated the expression of brain-derived neurotrophic factor (BDNF) and reduced the activity of BDNF-TrkB/pTrkB pathway in the hippocampus. These molecular changes were consistent with changes in synaptic-related proteins, suggesting that lycopene may enhance synaptic plasticity via the BDNF-TrkB/pTrkB signaling pathway. This study explored the mechanisms underlying depressive-like behaviors induced by CSDS in mice and provided preclinical evidence that lycopene may serve as a potential antidepressant. It offers an effective avenue for the development of novel antidepressant therapies.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 1","pages":"e70003"},"PeriodicalIF":3.5,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751711/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H K Wakhungu, G Abong, C Muthike, N Mutono, J Muema, G P Omondi, S M Thumbi, Z Bukania
{"title":"Factors Influencing Iron Levels Among Women of Reproductive Age and Children 6-48 Months in Livestock-Keeping Communities in Narok County, Kenya.","authors":"H K Wakhungu, G Abong, C Muthike, N Mutono, J Muema, G P Omondi, S M Thumbi, Z Bukania","doi":"10.1002/fsn3.4625","DOIUrl":"10.1002/fsn3.4625","url":null,"abstract":"<p><p>Proper nutrition is vital for maintaining good health for all people across their lifespan, especially children and mothers, who are especially vulnerable due to their specific nutrient needs. Despite the necessity of improved nutrition for these groups, some members do not fully meet their recommended daily micronutrient needs, a challenge exacerbated by different socioeconomic, cultural, and communal constraints resulting in malnutrition. Iron deficiency anaemia is a major concern among children and mothers, especially in pastoralist communities, due to poor nutrition and other related factors. Using a community-based cross sectional study, this study investigated factors associated with hemoglobin levels among children and women in Narok County, Kenya. Anthropometrics were estimated using body mass index measurements for mothers, and the nutritional status of children was calculated using <i>Z</i>-score measurements. Haemoglobin was measured using a rapid test (Hemocue 301). Multiple logistic regression models were fitted to assess the association between child and maternal risk factors and anaemia. Anaemia in children was associated with age (OR = 1.99, <i>p</i> = 0.047), pastoralism (OR = 2.25, <i>p</i> = 0.002), educational of the mother (OR = 0.74, <i>p</i> = 0.008), severe and moderate undernourishment (OR = 1.14, <i>p</i> = 0.049 and OR = 1.10, <i>p</i> = 0.023), respectively, not meeting children dietary diversity (OR = 1.18, <i>p</i> = 0.027), number of people in a household (OR = 1.84, <i>p</i> = 0.003), and maternal age (OR = 0.30, <i>p</i> = 0.010). On the other hand, the occurrence of anaemia in women was associated with pastoralism (OR = 2.22, <i>p</i> = 0.001), having a primary school level of education (OR = 0.51, <i>p</i> = 0.028), pregnancy status (OR = 5.36, <i>p</i> = 0.002), not meeting maternal dietary diversity (OR = 1.39, <i>p</i> = 0.026), number of household members (OR = 1.93, <i>p</i> = 0.023), age of the mother (OR = 0.53, <i>p</i> = 0.018), and having animals infected with East Coast Fever (<i>Theileria parva</i>) within the household (OR = 1.10, <i>p</i> = 0.023). The results highlight the multifaceted nature of malnutrition, specifically anaemia in pastoral households, with interventions aimed at reducing disease infections in cattle, improved household dietary diversity, and community health education geared towards maternal and child nutrition being best placed to improve the overall household health outcomes relating to anaemia.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 1","pages":"e4625"},"PeriodicalIF":3.5,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751623/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Azade Ghorbani, Ali Rafe, Mohammad Ali Hesarinejad, Jose M Lorenzo
{"title":"Impact of pH on the Physicochemical, Structural, and Techno-Functional Properties of Sesame Protein Isolate.","authors":"Azade Ghorbani, Ali Rafe, Mohammad Ali Hesarinejad, Jose M Lorenzo","doi":"10.1002/fsn3.4760","DOIUrl":"10.1002/fsn3.4760","url":null,"abstract":"<p><p>Sesame protein isolate (SPI) is a highly nutritious plant protein distinguished by its essential amino acid profile. This study investigates the influence of pH on SPI's physicochemical, structural, and techno-functional properties, highlighting its potential as a sustainable protein source for various food applications. Our findings revealed that SPI had a protein content of 90.60% and a protein extraction yield of 77.2%. The density is measured at 0.72 g/mL, with a critical compressibility index of 19.22, indicating excellent flowability for weaning foods. Notably, the ζ-potential shifts from +39 mV at pH 3.0 to 0 at the isoelectric point (pI, 5-5.5) and becomes negative at higher pH levels. We observed a direct correlation between solubility, fluorescence intensity, and functional characteristics of SPI, with peak solubility and functional properties at acidic and alkaline pH levels and lowest values at the pI. Structural analyses confirmed the relationship between electrical charge, hydrophobicity, and functional attributes, with the highest surface hydrophobicity observed at pH 2.0. In conclusion, our findings underscore the critical role of pH in modulating the physicochemical properties of sesame protein isolate, enhancing its applicability in food formulations. SPI demonstrates significant potential as a versatile ingredient in the functional food product development.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 1","pages":"e4760"},"PeriodicalIF":3.5,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143022923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}