Food Science & Nutrition最新文献

{"title":"Quality Changes in Chicken Meat Marinated With Antioxidant-Rich Fruit and Vegetable Juices","authors":"İlkay Çelik,&nbsp;Eda Alagöz,&nbsp;Hülya Şen Arslan,&nbsp;Cemalettin Sarıçoban","doi":"10.1002/fsn3.70135","DOIUrl":"https://doi.org/10.1002/fsn3.70135","url":null,"abstract":"<p>This study investigated the effects of marination with antioxidant-rich fruit juices—pomegranate, black carrot, and red beet—on the textural, physicochemical, technological, and sensory properties of chicken breast meat. Key parameters, including color, pH, water-holding capacity (WHC), marinade absorption, cooking loss (CL), textural properties, TBARS (thiobarbituric acid reactive substances), and sensory attributes, were evaluated. Among the tested juices, pomegranate had the highest acidity. Black carrot contained the highest total phenolic content (TPC) but showed the lowest ABTS and DPPH radical scavenging activity (<i>p</i> &lt; 0.05). Marination resulted in a reduction in pH, with the lowest values observed in pomegranate-marinated samples. Although marination influenced WHC, the changes were not statistically significant (<i>p</i> &gt; 0.05). CL values were significantly reduced in marinated samples (<i>p</i> &lt; 0.05). Marination also affected color, with red beet increasing the <i>b</i>* value and black carrot decreasing it (<i>p</i> &lt; 0.05). Textural properties, such as hardness, significantly increased with pomegranate and red beet juices (<i>p</i> &lt; 0.05), while other textural attributes remained unaffected (<i>p</i> &gt; 0.05). Sensory evaluation revealed no significant differences in flavor and texture, although color was notably influenced by the marination process (<i>p</i> &lt; 0.05). Control and RB gave the highest values in terms of general acceptability. These findings suggest that marination with pomegranate, black carrot, and red beet juices may contribute to improving antioxidative properties and improve the textural quality of chicken breast meat.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70135","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation of Enterocins Produced by Antilisterial Enterococcus faecium BH04, BH12, BH84, and BH99 and In Vitro/In Situ Inhibition of Listeria monocytogenes","authors":"Haktan Aktaş","doi":"10.1002/fsn3.70142","DOIUrl":"https://doi.org/10.1002/fsn3.70142","url":null,"abstract":"<p><i>Listeria monocytogenes</i> is a food pathogen that can cause fatal infections, especially in newborns, elderly individuals, and immunocompromised individuals. In recent years, research on novel antibiotics and natural antimicrobial agents of plant or microbial origin has become increasingly important in the face of multiple antibiotic resistance. In this study, enterocins produced by <i>Enterococcus faecium</i> BH04, BH12, BH84, and BH99 were investigated in vitro and in situ as antilisterial agents. The results showed that all strains had bacteriocin activity of 0.4 kAU/mL (400 AU/mL) after 3 h and 25.6 kAU/mL (25600 AU/mL) after 6 h in M17 broth. The strains inhibited the growth of <i>Listeria monocytogenes</i> ATCC 7644 in BHI. Furthermore, <i>E. faecium</i> BH04, BH12, BH84, and BH99 had bacteriostatic potential, whereas enterocins BH04, BH12, BH84, and BH99 had bactericidal potential in a milk model (in situ). The polymerase chain reaction results revealed that all strains had <i>entA</i>, <i>entB</i>, and <i>entP</i> genes encoding enterocin but not the <i>entL50</i> gene. The enterocins BH04, BH12, BH84, and BH99 retained their antilisterial activity up to 100°C (10 min), up to pH 10, and against catalase, lysozyme, and all chemicals used in this study. On the other hand, ultraviolet light reduced the antilisterial activity of enterocin BH12 by 75% and that of enterocin BH04, BH84, and BH99 by 50%. It was concluded that <i>E. faecium</i> BH04, BH12, BH84, and BH99 could be used as a co-culture in fermented products and that enterocins produced by the strains could be used as antilisterial agents.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70142","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Probiotics as Adjunctive Therapy to Fluoxetine on Depression Severity and Serum Brain-Derived Neurotrophic Factor, Cortisol, and Adrenocorticotropic Hormone in Patients With Major Depressive Disorder: A Randomized, Double-Blind, Placebo-Controlled Trial","authors":"Vajihe Elahinejad,&nbsp;Atie Sadat Khorasanian,&nbsp;Mehdi Tehrani-Doost,&nbsp;Kianoush Khosravi-Darani,&nbsp;Zahra Mirsepasi,&nbsp;Mohammad Effatpanah,&nbsp;Reza Askari-Rabori,&nbsp;Shirin Tajadod,&nbsp;Shima Jazayeri","doi":"10.1002/fsn3.4698","DOIUrl":"https://doi.org/10.1002/fsn3.4698","url":null,"abstract":"<p>Probiotics may improve mood, but their role as adjunctive therapy for major depressive disorder (MDD) is not well understood. This study examines the effects of probiotics on depression severity, brain-derived neurotrophic factor (BDNF), adrenocorticotropic hormone (ACTH), and cortisol levels in MDD patients. Fifty medication-free MDD patients were randomized to receive probiotics with fluoxetine (<i>n</i> = 25) or placebo with fluoxetine (<i>n</i> = 25) for 8 weeks. Depression severity was assessed using the Hamilton Depression Rating Scale (HDRS-24), and fasting blood samples were collected at baseline and study conclusion. Forty-four patients completed the trial. The probiotic group showed a significant reduction in depression severity compared with the placebo group (<i>p</i> = 0.001). No significant differences were observed in serum cortisol (<i>p</i> = 0.46) and ACTH levels (<i>p</i> = 0.44). Plasma BDNF levels increased slightly in the probiotic group but were not statistically significant. Probiotic supplementation with fluoxetine significantly reduces depression severity in MDD patients.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.4698","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Composition of Flavonoids and Nutritional Evaluation in Leaves of Different Sea-Buckthorn Germplasm Resources","authors":"Yue Yuan,&nbsp;Wentao Yao,&nbsp;Yuqi Wu,&nbsp;Rui Wang,&nbsp;Zeyuan Yu,&nbsp;Junwei Huo,&nbsp;Xingguo Li,&nbsp;Ke Tang","doi":"10.1002/fsn3.70013","DOIUrl":"https://doi.org/10.1002/fsn3.70013","url":null,"abstract":"<p>Sea-buckthorn leaves contain various active components, and research, development, and utilization of sea-buckthorn leaves have broad application prospects in the fields of food, medicine, and feed. The comprehensive quality of different sea-buckthorn germplasm resources was evaluated by determining 17 major nutrients, such as flavone composition, polysaccharides, total flavonoids, total polyphenols, and so on in 26 different varieties of sea buckthorn leaves, and then the comprehensive quality of different sea-buckthorn germplasm resources was evaluated by factor analysis, principal component analysis, and cluster analysis. The results showed that flavonols, mainly quercetin-like flavonols and isorhamnetin-like flavonols, were detected in the leaves of 26 sea-buckthorns. Through comprehensive analysis of its nutritional quality, it was found that the leaves of sea-buckthorn germplasm resources had highly significant genetic differences and germplasm diversity, and the total polyphenol content, polysaccharide content, VC content, total flavonoids content, and crude fat content of sea-buckthorn leaves were comparable to those of some commercially available high-quality famous teas. The higher crude protein content in the leaves of ‘S1’, the higher crude fiber content in the leaves of ‘S4’ and ‘S13’. The higher crude fat content in the leaves of ‘S8’ and the relatively higher polysaccharide content in the leaves of ‘S4’, ‘S22’, ‘S5’, ‘S18’ and ‘S7’ suggest to a certain extent that the leaves of these sea-buckthorn lines seem to serve as a botanical resource for the extraction of sea-buckthorn crude protein, crude fiber, crude fat, and polysaccharide, respectively. The contents of flavonoids in ‘S21’ and ‘S24’ leaves were relatively high, and the contents of total polyphenols in ‘S1’, ‘S8’ and ‘S21’ were relatively high, suggesting that these sea buckthorns could be used as plant resources for extracting flavonoids and total polyphenols from sea buckthorns to a certain extent. Factor analysis and principal component analysis showed that ‘S3’, ‘S16’, and ‘S10’ leaves had the highest comprehensive ranking. The results of this study laid a good foundation for the screening of sea buckthorn germplasm resources and the breeding, processing, and utilization of new varieties of sea-buckthorn leaves.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LC-ESI-QTOF-MS/MS Characterization of Phenolic Compounds in the Stem, Roots, and Leaves of Syzygium cumini and Their Antioxidant Potential","authors":"Ali Imran,&nbsp;Shujun Ye,&nbsp;Jiaying Amanda Li,&nbsp;Rahaf Ajaj,&nbsp;Abdur Rauf,&nbsp;Zubair Ahmad,&nbsp;Hassan A. Hemeg,&nbsp;Yahya Saleh Mohamed Al-Awthan,&nbsp;Omar S. Bahattab,&nbsp;Mohammed Mansour Quradha,&nbsp;Hafiz Suleria","doi":"10.1002/fsn3.70112","DOIUrl":"https://doi.org/10.1002/fsn3.70112","url":null,"abstract":"<p><i>Syzygium cumini</i>, commonly known as Jamun or Indian Blackberry, is a fruit-bearing tree native to the Indian subcontinent, revered for its medicinal uses, and characterized by a rich phytochemical profile abundant in polyphenols. This study aimed to characterize various fractions, including the roots, stem, and leaves of <i>S. cumini</i> (SC). Purposefully, conventional extraction was carried out by using ethanol (70%) with formic acid (1%) to extract the phytochemicals. The resultant extracts were subjected to phenolic contents (TPC, TFC, TTC) and antioxidant activity estimation (DPPH, FRAP, ABTS RPA, OH, FICA, and TAC). Further, LC-ESI-QTOF-MS/MS identification of phenolics was also performed. The outcomes showed that <i>S. cumini</i> leaf exhibited the highest phenolic content and antioxidant activity among different fractions compared to stem and root. The recorded TPC, TFC, and TTC in the leaf were 52.17 ± 1.60 mg GAE/g, 2.76 ± 0.054 mg QE/g, and 17.22 ± 0.43 mg <span>ce</span>/g, respectively. Correlation analysis revealed a strong positive correlation (0.50 &lt; <i>r</i> &lt; 0.80, <i>p</i> &lt; 0.01) between TPC, TFC, and TTC. The LC-ESI-QTOF-MS/MS screening showed the presence of 12 compounds in the stem, leaf, and root of <i>S. cumini</i>, showing the diversity of phenolics between different parts. The majority of the compounds belonged to flavonoids (4), phenolic acids (3), other polyphenols (3) and lignans (2). Among the notable compounds, naringin 4'-O-glucoside, 3,4-<i>O</i>-dimethylgallic acid, scutellarein, and demethyloleuropein were identified, highlighting the therapeutic potential of different fractions of <i>S. cumini.</i> In conclusion, the results indicated that SC fractions contained a considerable amount of phenolics, thus showcasing higher antioxidant activity. Moreover, the concentration of different phenolics varied among fractions, as confirmed through a Venn diagram.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70112","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping the Chemodiversity, Antioxidant and Enzyme Inhibitory Potential and In Silico Studies of Heliotropium europaeum","authors":"Shabnam Mustafa,&nbsp;Muhammad Imran Tousif,&nbsp;Naheed Raiz,&nbsp;Muhammad Saleem,&nbsp;Saba Tauseef,&nbsp;Gokhan Zengin,&nbsp;Laiba Hassan,&nbsp;Areeba Hassan,&nbsp;Mamona Nazir,&nbsp;Shabbir Muhammad","doi":"10.1002/fsn3.70119","DOIUrl":"https://doi.org/10.1002/fsn3.70119","url":null,"abstract":"<p>The annual plant <i>Heliotropium europaeum</i> grows in waste ground next to agricultural land and along roadsides from June to September. This plant is indigenous to Southern and Central Europe, as well as Western Asia and Northern Africa; the term “Europaeum” most likely refers to the continent of Europe. According to a literature search, <i>H. europaeum</i> is used externally to cure warts and to aid in wound healing. It also has antibacterial, antifungal, antitumor, anti-inflammatory, insecticidal, antispasmodic, cholagogue, emmenagogue, antipyretic, and anthelmintic properties. In the present investigation, we measured the biological activities of the extracts of <i>H. europaeum,</i> identification of secondary metabolites by UHPLC–MS/MS, and bioinformatics like docking studies against enzymes as evidence for the bioactivities. The results of chemical profiling showed that the HE-M fraction had the highest phenolic and flavonoid contents (15.63 ± 0.39 mg GAE/g extract and 5.67 ± 0.03 mg QE/g extract, respectively), while the HE-E fraction came in second (13.73 ± 0.35 mg GAE/g extract and 4.38 ± 0.08 mg QE/g extract, respectively). Similarly, out of all the extracts, the methanolic extract was found to have the highest antioxidant activity in DPPH and ABTS antioxidant assays, while the HE-E extract was found active among all the extracts in all other antioxidant assays. In the enzyme inhibition assay, we had mixed results, where the HE-E fraction was active against all the enzymes. The chemical profiling through UHPLC–MS/MS analysis endorsed the presence of many known pyrrolizidine alkaloids, flavonoids, and phenolic compounds, while the results of docking studies against the tested enzyme revealed the inhibitory action of pyrrolizidine alkaloids, flavonoids, and phenolic compounds. Further multivariate analysis was employed to find the relationship between total bioactive components and biological activities. This study established a foundation for utilizing these results and compounds as drug leads and for exploring the industrial applications of <i>H. europaeum</i> in developing products aimed at non-communicable diseases.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70119","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elderly Perception of Protein Products in Relation to Their Neophobic Attitude and Nutritional Protein Knowledge","authors":"Elizabeth Carrillo,&nbsp;Amparo Tárrega","doi":"10.1002/fsn3.70129","DOIUrl":"https://doi.org/10.1002/fsn3.70129","url":null,"abstract":"<p>This study examined the elderly response to protein-rich food measured in two contexts: Study 1, expected condition toward six commercial products; and Study 2, tasting the products (two protein breads) with special focus on the impact of individual food neophobia (FN) attitude and protein knowledge (PK). The initial study yielded findings indicating that the products were differentiated based on their similarity to the participants' usual dietary intake. Furthermore, differences were observed between participants with high and low levels of FN. In the first group, unfamiliar products were perceived as less healthy, less trustworthy, and more unusual than familiar products. Moreover, unfamiliar products elicited lower purchase intention. Similarly, low levels of PK also affected the perception of unfamiliar foods, albeit to a lesser extent. In Study 2, elderly individuals with a high level of FN also exhibited reduced purchase intention and a lower level of agreement that the product would be liked. Participants also perceived the products as less healthy, less satiating, and unsustainable for eating legumes. However, PK did not affect this response. These studies demonstrate the profound impact of FN on the aging process, influencing the rejection of protein-enriched products and impeding the ability to perceive the benefits of novel products.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70129","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Umami Enhancing Properties of Enzymatically Hydrolyzed Mycelium of Flammulina velutipes Cultured on Potato Pulp","authors":"Katharina Happel,&nbsp;Lea Zeller,&nbsp;Andreas Klaus Hammer,&nbsp;Holger Zorn","doi":"10.1002/fsn3.70128","DOIUrl":"https://doi.org/10.1002/fsn3.70128","url":null,"abstract":"<p>The aim of this study was to hydrolyze cultivated fungal mycelium and to evaluate the effect on its taste. Potato pulp, a by-product of the potato starch industry, was therefore successfully utilized as a substrate for submerged cultivation of <i>Flammulina velutipes</i>, yielding a product with an estimated fungal content of 83% ± 3%. The fermentation increased the protein content from 5.3 ± 0.4 g/100 g DM to 13.9 ± 0.1 g/100 g DM with a biological protein value of 86. The fermentate was enzymatically hydrolyzed by Corolase APC-peptidase. After optimization of the hydrolysis conditions, a degree of hydrolysis (DH) of 75.1% ± 1.0% was achieved. The protein hydrolysis increased the contents of free glutamate more than 20-fold from 8.7 ± 0.1 mg/L to 188.7 ± 1.2 mg/L. Elevated glutamate levels led to an umami taste perception in aqueous solution and taste-enhancing properties in vegetable broth. Noteworthy, the fermentate itself exhibited an intrinsic peptidase activity. Without addition of auxiliary peptidases, mycelial enzymes caused a DH of 33.9% ± 0.7% and a free glutamate content of 99.1 ± 0.7 mg/L. For these samples, an increase in umami taste was only observed in vegetable broth, but not in water, indicating taste-enhancing properties but low umami taste. In addition to the nutritional and health benefits of fungi, their hydrolysates are of great interest for use as a protein booster with flavor-enhancing properties.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70128","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypericum empetrifolium and H. lydium as Health Promoting Nutraceuticals: Assessing Their Role Combining In Vitro In Silico and Chemical Approaches","authors":"Stefania Sut,&nbsp;Stefano Dall'Acqua,&nbsp;Giancarlo Angeles Flores,&nbsp;Gaia Cusumano,&nbsp;İsmail Koyuncu,&nbsp;Ozgur Yuksekdag,&nbsp;Carla Emiliani,&nbsp;Roberto Venanzoni,&nbsp;Paola Angelini,&nbsp;Selami Selvi,&nbsp;Evren Yildiztugay,&nbsp;Adriano Mollica,&nbsp;Eleonora Procino,&nbsp;Gregorio Peron,&nbsp;Lorenza Marinaccio,&nbsp;Gokhan Zengin","doi":"10.1002/fsn3.70053","DOIUrl":"https://doi.org/10.1002/fsn3.70053","url":null,"abstract":"<p><i>Hypericum</i> species are known for their ability to produce multiple classes of secondary metabolite and in this article the possible health-promoting role of <i>H. empetrifolium</i> and <i>H. lydium</i> have been evaluated combining in vitro and in silico approaches. <i>H. empetrifolium</i> and <i>H. lydium</i> extracts were obtained using different solvents (ethyl acetate, aceton, aceton/water, and water) and the composition was compared using NMR and LC–MS based approaches. Myricetin-3-<i>O</i>-glucoside, Kaemempferol -3-<i>O</i>-glucoside, and hyperopliphylirrin were present only in <i>H. empetrifolium.</i> Rutin, quercetin-3-<i>O</i>-rhamnoside ant the triterpenoids oleanolic and ursolic acid were only detected in <i>H. lydium</i>. To establish a possible role against degenerative diseases antioxidant, antiradical activities of the extracts were studied and <i>H. empetrifolium</i> acetone/water and water extracts were the more active regarding these effects. The extracts were also evaluated for the inhibition of key-enzymes involved in degenerative diseases namely cholinesterase and tyrosinase for CNS-related pathologies and amylase for metabolic-related diseases. Significant inhibition of acetylcholinesterase was observed for the extracts obtained with lipophilic solvents. The extracts were also studied for their possible antiproliferative activity on cell lines including tumor (DU-145, A549, and MCF-7) and non-tumoral cells (HEK-293) revealing moderate activities. <i>H. lydium</i> ethyl acetate showed late apoptotic (17.5%) and necrotic (46.6%) effects in the Annexin V/PI assay. Molecular docking was used to establish possible interaction of identified compounds with target enzymes and a good interaction between rutin and tyrosinase, myricetin-7-<i>O</i>-glucoside and amylase, was reported. In conclusion, <i>Hypericum empetrifolium</i> and <i>H. lydium</i> thanks to their complex pattern of phytoconstituents and thanks to their significant antioxidant effects as well as with the ability to act on some key-target enzymes involved in degenerative diseases can be considered a good vegetal source for the preparation of nutraceuticals and food supplements useful as health-promoting products against oxidative stress-related diseases such as diabetes, cancer, and Alzheimer's disease.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70053","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143741661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Hybrid N-AHP-Based TOPSIS Decision Support Approach for Investigation of the Effect of Different Solvents on the Bioactive Properties, Anticancer, and Antimicrobial Activities of Aronia melanocarpa Extract","authors":"Gulsum Ucak Ozkaya","doi":"10.1002/fsn3.70122","DOIUrl":"https://doi.org/10.1002/fsn3.70122","url":null,"abstract":"<p>The aim of this study was to extract the bioactive components in <i>Aronia melanocarpa</i> L. fruit, commonly referred to as chokeberry, using various solvents (80% methanol + 1% formic acid, 80% ethanol, and 70% acetone) individually and to evaluate them through the Neutrosophic-Analytical Hierarchy Process (N-AHP)-based TOPSIS method. The ethanolic (EEA), methanolic (MEA), and acetone (AEA) extracts derived from Aronia fruit were analyzed for total phenolic, flavonoid, and anthocyanin contents, as well as antioxidant, antimicrobial, and cytotoxic activities. The EEA exhibited the highest overall anthocyanin content. The MEA exhibited the highest DPPH levels. The AEA exhibited the highest levels of total phenolic compounds, total flavonoid content, and CUPRAC values. The EEA, MEA, and AEA exhibited IC50 values of 11.65, 11.71, and 10.51 mg/mL against Caco-2 adenocarcinoma cells, respectively. EEA and MEA exhibited superior antimicrobial efficacy compared to AEA. According to the weights determined by N-AHP, the extraction with methanol was found to be the best extraction method according to the TOPSIS analysis results performed on real data. In other words, the extract with the lowest IC50 value and the highest antimicrobial activity was the one extracted with methanol. This study concluded that the N-AHP-based TOPSIS method is a significant multiple decision-making approach for evaluating plant extracts based on established criteria and is applicable to food and phytochemical sciences.</p>","PeriodicalId":12418,"journal":{"name":"Food Science & Nutrition","volume":"13 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/fsn3.70122","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}