Foodborne pathogens and disease最新文献

{"title":"Development of MNPs-PEI-GA-Nbs-qPCR Rapid Detection Method for <i>Vibrio fluvialis</i>.","authors":"Lingli Meng, Mengjuan Wu, Zhui Tu, Qinghua He, Yanping Li","doi":"10.1177/15353141251377326","DOIUrl":"10.1177/15353141251377326","url":null,"abstract":"<p><p><i>Vibrio fluvialis</i> is an emerging foodborne pathogen associated with severe infections. In this study, immunomagnetic beads (IMBs) were synthesized by conjugating nanobody N71 to magnetic nanoparticles (MNPs) via polyethylenimine (PEI) and glutaraldehyde (GA) cross-linking. Due to the high affinity of N71 for <i>V. fluvialis</i> lipopolysaccharide, the IMBs efficiently and specifically captured the target pathogen. When integrated with <i>ToxR</i>-targeted quantitative real-time PCR, this system achieved enhanced detection sensitivity (48 CFU/mL) and reduced false-positive rates. The optimization results showed that the capture efficiency of <i>V. fluvailis</i> reached its maximum (95%) when 125 µg of N71 were conjugated to 1 mg of MNPs to form the MNPs-PEI-GA-Nbs conjugates, achieved with a conjugate dosage of 0.5 mg and an incubation time of 45 min. Nontarget bacterial interference experiments and actual sample detections validated the excellent specificity of the method for detecting <i>V. fluvialis</i>. Compared with conventional culture-based methods, this method reduced detection time from 24-48 h to <7 h, providing an efficient and reliable alternative for rapid detection of <i>V. fluvialis</i> in food samples.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"678-685"},"PeriodicalIF":2.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145039480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Rapid Detection Method for <i>Salmonella</i> Based on Real-Time Recombinase Polymerase Amplification in Food.","authors":"Long Li, Liping Song, Qinglong Wang, Huatao Yin, Yiping Suo, Yujia Zhao, Qingyao Li, Yue Cao, Chao Xing, Dan Wang, Zhikai Hu, Jie Jiang","doi":"10.1177/15353141251380364","DOIUrl":"10.1177/15353141251380364","url":null,"abstract":"<p><p>This study developed a rapid detection method for <i>Salmonella</i> based on real-time recombinase polymerase amplification (real-time RPA). The method exhibited excellent specificity and could amplify target genes within 20 min at 39°C. It achieved a Limit of Detection (LOD₅₀) of 47 CFU/mL. To evaluate detection performance, artificially contaminated food samples-including egg products, chocolate products, meat products, grain-based products, and soy products-were tested. Prior to real-time RPA detection, the samples underwent an enrichment step by shaking incubation at 36°C for 6 h. The real-time RPA method demonstrated consistent and robust performance across diverse food matrices, with relative LOD (RLOD) values below 2.5, satisfying the validation criteria outlined in GUOBIAO 4789.45 (GB 4789.45). A chi-square test conducted on bulk pork samples further confirmed no significant difference between the real-time RPA method and the GB 4789.4 standard method (<i>p</i> > 0.05). These findings highlight the potential of real-time RPA as a reliable and efficient alternative to GB 4789.4 for detecting <i>Salmonella</i>, enhancing food safety monitoring practices.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"670-677"},"PeriodicalIF":2.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145130414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Porphyra tenera</i> an Edible Red Alga Inhibits Norovirus P Domain Binding to the Host Cell Receptor.","authors":"Hyojin Kim, Jeong Ah Lee, Yeongeun Lee, Kyung Hyun Kim, Mi Sook Chung","doi":"10.1177/15353141261418395","DOIUrl":"10.1177/15353141261418395","url":null,"abstract":"<p><p><i>Porphyra tenera</i> (PT), an edible red alga, is consumed as a health-promoting seaweed. Human norovirus (HuNV), the major cause of foodborne disease outbreaks worldwide, binds to histo-blood group antigens (HBGAs) for cell entry. We investigated the inhibitory activity of fucose-containing polysaccharides in PT on HuNV binding to the HBGAs. With saliva as a source of HBGAs and recombinant P domains of HuNV GII.4 and GII.17 purified as NV antigens, the inhibition and binding affinity of PT toward P domains were evaluated using enzyme-linked immunosorbent assay and bio-layer interferometry, respectively. The PT polysaccharide extract and its 3-10 kDa fraction (F3-10) among molecular weight fractions inhibited the P domain binding to saliva significantly, compared with that of a commercial fucoidan. F3-10 bound directly to the P domains with submicromolar affinities (K_D = 5.6 × 10^-7 M for GII.4 and 8.7 × 10^-7 M for GII.17). When applied to virus inhibition assays, F3-10 significantly reduced murine NV titers in a dose-dependent manner; specifically, it showed a 1.8 log₁₀ plaque forming unit (PFU)/mL reduction at 1 mg/mL in RAW 264.7 cells (<i>p</i> < 0.05) and a 1.4 log₁₀ PFU/mL reduction at 5 mg/mL under simulated human digestion conditions involving sequential incubation in simulated saliva, gastric, and intestinal fluids (<i>p</i> < 0.01). F3-10 contained galactose, glucose, fucose, and xylose as its constituent monosaccharides and also sulfate groups. The PT fraction F3-10 is a promising candidate for further study aimed at inhibiting HuNV binding to host cell HBGAs.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"686-692"},"PeriodicalIF":2.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146029040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Resistance Profiles of <i>Enterococcus faecium</i> and <i>Enterococcus faecalis</i> Isolated from Food Animal Carcasses in South Korea, 2014-2023.","authors":"Ji-Hyun Choi, Ji-In Kim, Md Sekendar Ali, Bo-Youn Moon, Hee-Seung Kang, Yu-Jeong Hwang, Yeon-Hee Lee, Hyeon-Jung Park, Ha-Young Kim, Jae-Myung Kim, Suk-Kyung Lim","doi":"10.1177/15353141251380373","DOIUrl":"10.1177/15353141251380373","url":null,"abstract":"<p><p>Antimicrobial resistance in livestock products poses an important public health threat globally. This study aimed to evaluate the antimicrobial resistance profiles and trends of <i>Enterococcus faecium</i> and <i>Enterococcus faecalis</i> isolated from food animal carcass samples during 2014-2023 in South Korea. The obtained 1632 <i>E. faecium</i> and 3493 <i>E. faecalis</i> isolates from cattle, pig, and chicken carcasses at the national level were tested for antimicrobial susceptibility using broth microdilution. <i>E. faecium</i> and <i>E. faecalis</i> isolates from chickens showed higher resistance rates to most evaluated antimicrobials compared with cattle or pig strains. <i>E. faecium</i> isolated from chickens showed high levels of resistance to ciprofloxacin, erythromycin, and tetracycline by >50%. Likewise, over 40% of the <i>E. faecalis</i> isolates recovered from chickens exhibited resistance to these antimicrobials. Of note, very few or no enterococci isolates were resistant to ampicillin, gentamicin, linezolid, and vancomycin. Furthermore, notwithstanding fluctuations, enterococci strains showed an increasing resistance trend to some antimicrobials, including erythromycin and streptomycin. <i>E. faecium</i> exhibited significantly greater multidrug resistance (MDR) rates in chickens compared with cattle and pig isolates, while MDR <i>E. faecalis</i> was higher in pigs and chickens than in cattle isolates (<i>p</i> < 0.05). The main components of the resistance patterns were erythromycin, ciprofloxacin, or tetracycline. Moreover, MDR patterns in <i>E. faecalis</i> (23.4%) and <i>E. faecium</i> (17.8%) include five or more antimicrobials. The MDR enterococci contaminating carcasses during the slaughtering of food animals can be spread to humans through the food chain, posing a potential hazard to public health. Thus, judicious use of antimicrobials and proper sanitary measures are essential to mitigate the risk of transmission.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"657-669"},"PeriodicalIF":2.3,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence and Genomic Characterization of <i>Listeria monocytogenes</i> ST121 in Animal-Derived Food Samples from Bejaïa, Algeria.","authors":"Assia Mairi, Sylvain Meyer, Valentin Tilloy, Olivier Barraud, Abdelaziz Touati","doi":"10.1177/15353141261489403","DOIUrl":"https://doi.org/10.1177/15353141261489403","url":null,"abstract":"<p><p><i>Listeria monocytogenesis</i> an important foodborne pathogen that can cause severe invasive disease in vulnerable populations. This study assessed the occurrence of <i>L. monocytogenes</i> in animal-derived foods collected in Bejaïa, Algeria, and characterized recovered isolates using whole-genome sequencing (WGS). A total of 553 samples (red meat, minced meat, sausage, chicken carcasses, raw milk, and artisanal butter) were analyzed using ISO 11290-based culture, with species confirmation by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Twenty-two isolates were recovered (overall prevalence 4% ± 1.6), with the highest positivity in chicken carcasses (10.9% ± 5.8, 12/110) and artisanal butter (12.5% ± 16.2, 2/16). All isolates belonged to sequence type ST121 and carried the same plasmid replicon rep26. No acquired antimicrobial resistance genes were identified in any isolate. Screening of virulence-associated loci indicated genomic carriage of all conserved set of loci. A truncation in <i>inlA</i> gene was present, which has been associated with decreased virulence pattern. These findings highlight the occurrence of <i>L. monocytogenes</i> in multiple animal-derived products in Bejaïa and support the integration of WGS into routine food-chain surveillance.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"15353141261489403"},"PeriodicalIF":2.3,"publicationDate":"2026-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149029825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence, Enterotoxin Gene Profiles, Psychrotrophic Characteristics, and Antibiotic Resistance of <i>Bacillus cereus</i> Isolated from Ready-to-Eat Foods.","authors":"Cansu Kinali, Özgür Çadırcı","doi":"10.1177/15353141261489411","DOIUrl":"https://doi.org/10.1177/15353141261489411","url":null,"abstract":"<p><p><i>Bacillus cereus</i> is an endospore-forming opportunistic pathogen with global distribution, responsible for two distinct gastrointestinal illness types. The present study aimed to determine the occurrence of <i>B. cereus</i> in ready-to-eat (RTE) foods marketed in Samsun, Turkey, and to characterize confirmed isolates with respect to enterotoxin gene carriage, capacity for psychrotrophic growth, and antibiotic susceptibility profiles. One hundred fifty RTE food samples were examined, including spicy paste (<i>acılı ezme</i>, <i>n</i> = 29) Russian salad (<i>rus salatası</i>, <i>n</i> = 44), raw meatballs (<i>çiğ köfte</i>, <i>n</i> = 42), and stuffed grape leaves (<i>yaprak sarma</i>, <i>n</i> = 35), procured from retail outlets and food service establishments between December 23, 2024, and February 28, 2025, and analyzed in accordance with TS EN ISO 7932:2004. <i>B. cereus</i> was recovered from 30.6% (46/150) of samples overall, with the greatest contamination frequencies recorded in spicy paste (62.1%, 18/29) and <i>çiğ köfte</i> (57.1%, 24/42). Viable counts in contaminated samples fell within the range of 1.0 × 10¹ to 4.1 × 10³ colony-forming units (CFU)/g. Multiplex polymerase chain reaction screening of the 46 confirmed isolates showed that 28.2% (13/46) carried at least one enterotoxin gene; <i>hbl</i>, <i>nhe</i>, and <i>cytK</i> were each identified in 13.0% of isolates, whereas the emetic toxin gene <i>ces</i> was not detected in any isolate. Ability to proliferate at 7°C was demonstrated in 52.2% (24/46) of isolates. Kirby-Bauer disk diffusion testing revealed that all isolates (100%) were resistant to penicillin, with resistance also recorded for ampicillin (38.5%), tetracycline (30.8%), and clindamycin (23.1%). Collectively, these findings underscore the public health relevance of enterotoxin-carrying, cold-tolerant, and antibiotic-resistant <i>B. cereus</i> in RTE foods and emphasize the necessity of strengthening temperature management and sanitation measures at all stages of the food supply chain.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"15353141261489411"},"PeriodicalIF":2.3,"publicationDate":"2026-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149012011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence and Integrated Food-Safety Characterization of <i>Staphylococcus aureus</i> in Retail Raw Meat from Northern Xinjiang, China.","authors":"Tianshuai Yuan, Han Sun, Xin Wang, Saihui Sui, Yu Duan, Wenyi Ma, Yue Xu, Baokun Li, Juan Dong, Hua Ji","doi":"10.1177/15353141261489407","DOIUrl":"https://doi.org/10.1177/15353141261489407","url":null,"abstract":"<p><p>In this study, 327 retail raw meat samples collected from five cities were examined for <i>Staphylococcus aureus</i>, and 92 isolates recovered from 73 positive samples were further characterized for classical enterotoxin genes and toxin production, antimicrobial susceptibility, biofilm formation, and molecular typing. <i>S. aureus</i> was detected in 22.3% of samples (73/327), with positivity varying across the sampled cities and meat types. Classical enterotoxin genes were detected in 10.9% of isolates (10/92), and 9 of the 10 gene-positive isolates produced at least one detectable classical enterotoxin. Resistance was highest to penicillin (94.6%), erythromycin (71.7%), tetracycline (59.8%), and clindamycin (58.7%); 80.4% of isolates were multidrug-resistant, and 19.6% carried the <i>mecA</i> gene. Moderate or strong biofilm formation was observed in 93.5% of isolates. Among a purposively selected subset of 41 isolates used for molecular typing, <i>spa</i> type t236 and pulsed-field gel electrophoresis cluster P2 were the most frequently observed profiles. Overall, retail raw meat sampled in Northern Xinjiang harbored <i>S. aureus</i> with confirmed toxigenic potential in a subset, frequent multidrug resistance, and widespread biofilm-forming ability. Together, these results provide an integrated evidence base for prioritizing antimicrobial resistance and toxin surveillance alongside hygiene control in retail meat systems.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"15353141261489407"},"PeriodicalIF":2.3,"publicationDate":"2026-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149012031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Corrigendum to:</i> Economic Burden of Foodborne Illnesses Acquired in the United States.","authors":"","doi":"10.1177/15353141261485767","DOIUrl":"https://doi.org/10.1177/15353141261485767","url":null,"abstract":"","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"15353141261485767"},"PeriodicalIF":2.3,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148976173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short-Term Meteorological Variability and Pathogen-Specific Foodborne Disease Risk in Ningbo, China.","authors":"Yichao Dai, Danjie Jiang, Beibei Lu, Yan Zhang, Keqin Ding, Dandan Zhang, Mengling Tang, Mingjuan Jin, Kun Chen, Jianbing Wang","doi":"10.1177/15353141261485768","DOIUrl":"https://doi.org/10.1177/15353141261485768","url":null,"abstract":"<p><p>Meteorological factors may influence the risk of foodborne diseases (FBDs), but evidence comparing different case definitions and pathogen-specific associations within the same surveillance system remains limited. This study aimed to investigate the relationship between short-term exposure to meteorological factors and FBDs in Ningbo, China. We used data from the Ningbo Foodborne Disease Surveillance System and the daily meteorological data of the Ningbo Meteorological Bureau for time-series analysis. Generalized additive models and distributed lag nonlinear models were used to evaluate the associations adjusted for long-term trends, seasonal changes, day of week, and holidays. A stratified analysis was carried out by sex, age, and pathogens. We found that short-term temperature rise was significantly associated with risk of clinically diagnosed FBD cases (mean: relative risks [RR] = 1.028, 95% confidence interval [CI]: 1.006-1.049; minimum: RR = 1.025, 95% CI: 1.004-1.047). Sex- and age-stratified analyses indicated positive temperature associations in both sexes and in several age groups between 15 and 64 years. Pathogen-specific analyses show that there is a positive relationship for <i>Vibrio parahaemolyticus</i> (mean temperature: RR = 1.087, 95% CI: 1.021-1.157), whereas an inverse relationship for Norovirus (mean temperature: RR = 0.965, 95% CI: 0.938-0.993). The findings remained robust in sensitivity analyses. These findings may inform targeted seasonal and pathogen-specific surveillance.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"15353141261485768"},"PeriodicalIF":2.3,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148873584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Salmonella</i> Enteritidis Outbreak Associated with Contaminated Raw Eggs in Cold-Braised Meat Assortment: Changsha, China, 2024.","authors":"Dihui Ma, Bin Tian, Xu Hengbo, Wei Tang, Miao Li, Liuqing Yang, Yingchun Song, Keke Cao, Heng Zhang, Yu Liao, Sun Jian, Jinfu Zhang, Ying Gao, Canghai Zhou","doi":"10.1177/15353141251359514","DOIUrl":"10.1177/15353141251359514","url":null,"abstract":"<p><p><i>Salmonella</i>, a significant zoonotic pathogen, remains the predominant global etiological agent of foodborne diseases and consistently ranks as one of the leading causes of foodborne disease outbreaks in China. We report a foodborne disease outbreak caused by <i>Salmonella</i> Enteritidis contamination of a cold-braised meat assortment (including a beef shank, pork tongue, tripe, marinated eggs, and dried egg products) linked to contaminated raw eggs at the JN Ecological Farm in Changsha City, Hunan Province, China, in November 2024. The outbreak was traced to a birthday lunch banquet on November 11, among 165 attendees, 54 confirmed cases were identified. The clinical manifestations primarily included diarrhea (79.63%), abdominal pain (75.93%), fever (40.74%), vomiting (24.07%), and nausea (22.22%), with an incubation period ranging from 5 to 36 h (median: 15 h). Notably, 52 of the 54 cases required hospitalization, including one critical case. Laboratory confirmation was obtained in 16 cases through bacterial isolation, with concurrent detection of <i>Salmonella</i> in residual braised meat assortment samples. Whole-genome sequencing was performed on all isolates, and subsequent bioinformatics analysis revealed a high degree of genetic homology, with fewer than three single-nucleotide polymorphisms identified among the isolates.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"595-601"},"PeriodicalIF":2.3,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144642172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}