Foodborne pathogens and disease最新文献

{"title":"Genetic Diversity, Antimicrobial Resistance, and Virulence Profiles of <i>Listeria monocytogenes</i> Isolates from Nantong, China (2020-2023).","authors":"Qin Shi, Xin Zhang, Di Wang, Weibing Zhang, Xiuxiu Jin, Yihua Sun, Ailong Huang","doi":"10.1089/fpd.2025.0027","DOIUrl":"https://doi.org/10.1089/fpd.2025.0027","url":null,"abstract":"<p><p><i>Listeria monocytogenes</i> poses significant public health and food safety risks due to its environmental resilience and pathogenicity. In this study, we utilized whole-genome sequencing to characterize 15 <i>L. monocytogenes</i> strains isolated from Nantong, China (2020-2023), recovered from food and clinical samples. Antimicrobial susceptibility testing revealed that the Nantong isolates exhibited high resistance rates to ciprofloxacin (93.3%) and oxacillin (66.6%). Of particular concern, isolate NTLM03 exhibited the most extensive resistance profile, demonstrating resistance to six antimicrobials, including clindamycin, trimethoprim/sulfamethoxazole, erythromycin, chloramphenicol, ciprofloxacin, and tetracycline. Seven distinct multilocus sequence types were identified, with ST9 being the most prevalent. Virulence analysis revealed premature stop codons in the <i>inlA</i> gene of all ST9 isolates, a marker indicative of reduced virulence. In contrast, ST87 isolates carried the full-length <i>inlA</i> gene and the complete <i>Listeria</i> Pathogenicity Island-4 (<i>LM9005581_70009</i> to <i>LM9005581_70014</i>), associated with high virulence. Core genome single nucleotide polymorphism (cgSNP) analysis demonstrated close relatedness (SNPs <20) among isolates from the same batch of meat products (NTLM03, NTLM04, NTLM05), suggesting a potential common contamination source. Furthermore, we examined the genetic relatedness of Nantong isolates with domestic and international <i>L. monocytogenes</i> strains. Two characteristic <i>L. monocytogenes</i> plasmids were assembled: the multidrug-resistant (MDR) plasmid pNTLM03 and the cadmium-resistant plasmid pNTLM08. Surprisingly, pNTLM03 shared a highly similar MDR region with <i>Listeria innocua</i> plasmids pLI42 and pLI203 from Chinese food sources. The transfer of antimicrobial resistance genes between <i>Listeria</i> species and other genera through various genetic mechanisms may increase the potential for the evolution of resistant <i>L. monocytogenes</i> strains, thereby increasing the difficulty of clinical treatment.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143961167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of the COVID-19 Pandemic on Foodborne Disease Healthcare-Seeking Behavior and Diagnoses at a Large Academic Medical System.","authors":"James A Barkley, Robert L Scharff, Joan-Miquel Balada-Llasat, Barbara Kowalcyk","doi":"10.1089/fpd.2023.0092","DOIUrl":"10.1089/fpd.2023.0092","url":null,"abstract":"<p><p>The objective of this study was to examine changes in healthcare-seeking behaviors and diagnostic practices around foodborne illness during the COVID-19 pandemic in a large university-based health system. A retrospective cohort study of individuals diagnosed with pathogens commonly transmitted through food between 2015 and 2020 was undertaken using electronic medical record data. Regression models were used to compare measured incidence rates of various foodborne pathogens as well as associated healthcare-seeking behaviors during the pandemic year of 2020 to previous years. Incidence of campylobacteriosis, cholera, and norovirus in 2020 significantly decreased, respectively, by 65.5% (<i>p</i> < 0.01), 90.1% (<i>p</i> = 0.02), and 73.0% (<i>p</i> = 0.03) compared with an average from 2017- to 019. Average annual visits for patients included in our sample significantly increased by 8.0% when comparing the average from 2017-2019 to 2020 (<i>p</i> < 0.01). These results suggest that the pandemic impacted healthcare use related to foodborne disease either due to reduced exposure to foodborne pathogens or reduced willingness to seek healthcare.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"316-321"},"PeriodicalIF":1.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142125265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alcohol in Baijiu Contributes to the Increased Probability of Host Infection by <i>Clostridioides difficile</i> Spores.","authors":"Wen Rui, Saiwei Zhong, Xiaoqian Li, Caihong Shen, Xiaonian Cao, Jingpeng Yang","doi":"10.1089/fpd.2023.0187","DOIUrl":"10.1089/fpd.2023.0187","url":null,"abstract":"<p><p><i>Clostridioides difficile</i> and its endospores possess the characteristics of a foodborne pathogen and have been detected at several stages in the food chain. In the presence of an imbalance in host intestinal ecology, <i>C. difficile</i> can proliferate and cause intestinal infections. Multiple food source factors can substantially alter the host's gut ecosystem, including the consumption of baijiu. However, it remains to be known whether the gut ecological changes induced by the consumption of baijiu increase the risk of <i>C. difficile</i> invasion and infection. In this study, <i>C. difficile</i> cells were exposed to two commercially available baijiu to evaluate the effect of baijiu on <i>C. difficile</i> cells and to verify through a mouse model. The results showed that baijiu effectively inhibited the growth and biofilm production of <i>C. difficile</i>, downregulated the expression levels of <i>tcdA</i> and <i>tcdB</i> virulence genes but upregulated the expression level of spore-producing genes <i>Spo0A</i>, enhanced the spore production, as well as increased <i>C. difficile</i> cell adhesion to Caco-2 cells. The mouse model showed that the intake of baijiu promoted the invasion and infection of <i>C. difficile</i> spores, causing damage to the cecum tissue, accompanied by an increase in the gut lipid carrier protein-2 (Lcn-2) and TcdA toxin protein levels. Simultaneously, cholic acid was elevated, whereas deoxycholic acid was decreased. This study is the first to find a possible link between baijiu intake and <i>C. difficile</i> spore invasion and infection.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"350-358"},"PeriodicalIF":1.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141758128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycobiota, Total Aflatoxins, And Ochratoxin A of Black And Green Cumin.","authors":"Helal F Al-Harthi","doi":"10.1089/fpd.2024.0096","DOIUrl":"10.1089/fpd.2024.0096","url":null,"abstract":"<p><p>Because of the medical importance of cumin as well as it being one of the food additives to many Saudi dishes, there was a need to study the fungal load of this type of spice. This study aimed to determine the mycological profile of the retail black and green cumin distributed in different markets at western region, Saudi Arabia, using the dilution plat method on dichloran 18% glycerol (DG18) agar and incubation at 25°C. Using morphological criteria and molecular markers (internal transcribed spacer sequence), 39 species belonging to 18 genera were collected from different black cumin (33 species belonging to 17 genera) and green cumin (25 species belonging to 9 genera). <i>Alternaria alternata, Aspergillus flavus, A. niger, A. ochraceus, Cladosporium cladosporioides,</i> and <i>Stemphylium botryosum</i> were the most prevalent. Black cumin harbors fungal counts reaching 545 colony-forming units (CFU)/g, while green cumin included 500 CFU/g. Also, the natural occurrence of aflatoxins and ochratoxin A was also measured. Seventy-two cumin samples (90% of tested samples) showed toxin contamination. Aflatoxins and ochratoxin A ranged from 9.35 to 3.9 PPB in black cumin samples and from 4.08 to 5.75 PPB in green cumin samples.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"359-366"},"PeriodicalIF":1.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142125266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of Pathogenic <i>Escherichia coli</i> Attachment to Meat.","authors":"Nicolae Corcionivoschi, Igori Balta, David McCleery, Iulia Bundurus, Ioan Pet, Todd Calaway, Ileana Nichita, Lavinia Stef, Sorin Morariu","doi":"10.1089/fpd.2023.0164","DOIUrl":"10.1089/fpd.2023.0164","url":null,"abstract":"<p><p><i>Escherichia coli</i> are present in the human and animal microbiome as facultative anaerobes and are viewed as an integral part of the whole gastrointestinal environment. In certain circumstances, some species can also become opportunistic pathogens responsible for severe infections in humans. These infections are caused by the enterotoxinogenic <i>E. coli</i>, enteroinvasive <i>E. coli</i>, enteropathogenic <i>E. coli</i> and the enterohemorrhagic <i>E. coli</i> species, frequently present in food products and on food matrices. Severe human infections can be caused by consumption of meat contaminated upon exposure to animal feces, and as such, farm animals are considered to be a natural reservoir. The mechanisms by which these four major species of <i>E. coli</i> adhere and persist in meat postslaughter are of major interest to public health and food processors given their frequent involvement in foodborne outbreaks. This review aims to structure and provide an update on the mechanistic roles of environmental factors, curli, type I and type IV pili on <i>E. coli</i> adherence/interaction with meat postslaughter. Furthermore, we emphasize on the importance of bacterial surface structures, which can be used in designing interventions to enhance food safety and protect public health by reducing the burden of foodborne illnesses.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"339-349"},"PeriodicalIF":1.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140874574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcontinental Dissemination of Enterobacterales Harboring <i>bla</i>_NDM-1 in Retail Frozen Shrimp.","authors":"Elizabeth M Parker, Dixie F Mollenkopf, Gregory A Ballash, Cong Li, Thomas E Wittum","doi":"10.1089/fpd.2023.0161","DOIUrl":"10.1089/fpd.2023.0161","url":null,"abstract":"<p><p>The global food trade provides a means of disseminating antimicrobial resistant (AMR) bacteria and genes. Using selective media, carbapenem-resistant species of Enterobacterales (<i>Providencia</i> sp. and <i>Citrobacter</i> sp.), were detected in a single package of imported frozen shrimp purchased from a grocery store in Ohio, USA. Polymerase chain reaction confirmed that both isolates harbored <i>bla</i>_NDM-1 genes. Following PacBio long read sequencing, the sequences were annotated using the NCBI Prokaryotic Genome Annotation Pipeline. The <i>bla</i>_NDM-1 genes were found in IncC plasmids, each with different antimicrobial resistance island configuration. We found that the <i>bla</i>_NDM-1 AMR islands had close relationships with previously reported environmental, food, and clinical isolates detected in Asia and the United States, highlighting the importance of the food chain in the global dissemination of antimicrobial resistance.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"332-338"},"PeriodicalIF":1.9,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rectal Perforation Induced by <i>Taenia saginata</i>: A Case Report.","authors":"Nurhilal Kızıltoprak, Ayşe Gökçen Yıldız, Gülçin Ercan","doi":"10.1089/fpd.2025.0008","DOIUrl":"https://doi.org/10.1089/fpd.2025.0008","url":null,"abstract":"<p><p><i>Taenia saginata</i>, commonly known as the beef tapeworm, is a prevalent zoonotic cestode with an estimated global prevalence of 60-70 million carriers. This parasite is frequently located in the gastrointestinal tract, and previous reports have described complications such as acute appendicitis, Meckel's diverticulitis, pancreatitis, cholecystitis, liver abscess, intestinal obstruction, and perforation. Intestinal perforation due to parasitic diseases is a rare complication. Surgical complications associated with <i>T. saginata</i> are uncommon, with few reported cases of bowel perforation. Here, we present the case of a 69-year-old female who was admitted to the emergency department with acute abdominal symptoms. She had experienced abdominal pain and vomiting for 15 days. Her symptoms were accompanied by abdominal distension, reduced bowel movements, and loss of appetite. On physical examination, she had diffuse abdominal tenderness, guarding, and rebound tenderness, raising suspicion of peritonitis. Laboratory tests revealed a white blood cell count of 8,000/mm³ and an elevated C-reactive protein level of 501 mg/L, indicating an ongoing inflammatory response. Imaging revealed free intra-abdominal air and fluid, suggesting gastrointestinal perforation. During emergency surgery, a perforation in the upper rectum was identified and resected. Pathological examination confirmed the presence of <i>T. saginata</i> eggs in the intestinal wall. Postoperatively, the patient recovered well and was discharged on the sixth day. This case underscores the need for heightened awareness of parasitic infections as potential causes of acute abdominal conditions. Proper diagnosis and prompt surgical intervention are crucial in managing complications associated with <i>T. saginata</i>. Preventive measures, including thorough cooking of beef and improved sanitation, are essential to reduce the incidence of such infections.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of <i>Salmonella</i> Detection in Garlic Granules Using Neutralizer-Aided Enrichment and PMAxx-qPCR.","authors":"Bingkui Li, Yuxin Zheng, Yawen Huang, Jiahao Ji, Shaoting Li, Hongmei Zhang","doi":"10.1089/fpd.2025.0024","DOIUrl":"https://doi.org/10.1089/fpd.2025.0024","url":null,"abstract":"<p><p>Spices with low water activity (a_w) often contain antibacterial substances that complicate the detection of <i>Salmonella</i> using traditional culture methods. These substances can drive <i>Salmonella</i> into a viable but nonculturable (VBNC) state, rendering it undetectable with culture-based methods. This study aimed to enhance the broth for enriching <i>Salmonella</i> in garlic granules by mitigating the inhibitory effects of antibacterial substances. This study optimized the enrichment broth by using 3× buffered peptone water to increase the buffering capacity of the medium, adding magnesium sulfate and sodium pyruvate to promote the self-repair and recovery of bacteria, and adding DL-dithiothreitol (DTT) and corn oil to neutralize the antimicrobial substances in garlic. Garlic granules were artificially contaminated with <i>Salmonella</i> and subjected to a 2-week desiccation process to simulate low a_w conditions during food storage. The results showed that adding DTT and corn oil as neutralizers in the modified buffered peptone water (mBPW) broth significantly enhanced the detection capability of <i>Salmonella</i> in garlic granules, enabling detection of <i>Salmonella</i> at initial contamination levels as low as 5 CFU/g. In contrast, <i>Salmonella</i> was undetectable without the addition of neutralizers during enrichment. In addition, the optimized mBPW broth was tested on spices other than garlic granules, demonstrating its effectiveness (without neutralizers) in detecting <i>Salmonella</i> in star anise and black pepper. Notably, this study demonstrated that the combination of neutralizer-aided enrichment and PMAxx-qPCR successfully enabled the detection of viable <i>Salmonella</i> in garlic granules. The entire process can be completed in approximately 26 h, demonstrating the method's swiftness and effectiveness in detecting viable cells. This approach provides a fast and sensitive method for the detection of <i>Salmonella</i> in garlic granules, with promising potential for application in similar inhibitory food matrices.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Detection of <i>Salmonella</i> and <i>Listeria monocytogenes</i> in Fresh Beef Using Loop-Mediated Isothermal Amplification.","authors":"Xiaoran Wang, Kaixiang Jia, Jinrong Ran, Yangyang Du, Xiaoying Yu, Xuefeng Cao, Lianci Peng, Weifeng Chen, Rendong Fang, Zhiwei Li","doi":"10.1089/fpd.2025.0018","DOIUrl":"https://doi.org/10.1089/fpd.2025.0018","url":null,"abstract":"<p><p>This study developed and optimized loop-mediated isothermal amplification (LAMP) assays for rapid detection of <i>Salmonella</i> and <i>L. monocytogenes</i> in fresh beef. LAMP primers targeting the <i>invA</i> gene of <i>Salmonella</i> and <i>hly</i> gene of <i>L. monocytogenes</i> were used. Reaction conditions including temperature, dNTP concentration, Mg²⁺ concentration, primer ratio, and Bst DNA polymerase amount were optimized for each pathogen. Differences were observed between the two pathogens in optimal temperature, Mg²⁺ concentration, and Bst DNA polymerase requirements, highlighting the importance of pathogen-specific optimization. The optimized assays demonstrated high sensitivity with detection limits of 120 fg/μL for <i>Salmonella</i> and 130 fg/μL for <i>L. monocytogenes</i>, achieving detection within 40 and 60 min, respectively. Specificity tests confirmed both assays were highly specific for their target pathogens in fresh beef samples with no cross-reactivity observed. Addition of hydroxynaphthol blue enabled simple visual detection of positive results, eliminating the need for specialized equipment. The developed LAMP assays offer rapid, sensitive, and specific detection of these important foodborne pathogens.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144063040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection and Antibiotic Susceptibility Profile of Bacterial Pathogens Isolated from Food Cutting Boards in Rio De Janeiro, Brazil.","authors":"Bianca de Souza Costa, Katarine Coutinho Silva, Carolaine Totelote Medeiros Pimenta Rodrigues, Ana Carolina Carvalho De Oliveira, Silvia Maria Dos Reis Lopes, Ivano de Filippis","doi":"10.1089/fpd.2024.0182","DOIUrl":"https://doi.org/10.1089/fpd.2024.0182","url":null,"abstract":"<p><p>We investigated bacterial pathogens on restaurant cutting boards using phenotypic and molecular methods, and determined their antibiotic susceptibility. A total of 32 samples were collected from polyethylene cutting boards in the kitchens of 16 restaurants in Rio de Janeiro, Brazil. A total of 29 bacterial strains were isolated and identified, including <i>Salmonella</i> spp. (<i>n</i> = 11), <i>Escherichia coli</i> (<i>n</i> = 10), <i>Staphylococcus aureus</i> (<i>n</i> = 6), and <i>Listeria</i> spp. (<i>n</i> = 2). Of the 32 cutting boards, 9 (28.1%) harbored at least one bacterial species, 7 (21.9%) had two species, and 2 (6.2%) carried three species. Notably, the detection of two pathogens directly from raw samples using quantitative polymerase chain reaction with high-resolution melting (qPCR-HRM) was significant: <i>Listeria monocytogenes</i> was identified in two samples by phenotypic methods but in 18 samples by qPCR-HRM, while <i>E. coli</i> was found in 10 samples by phenotypic methods and in 29 samples by qPCR-HRM. Antibiotic resistance was another concern, especially for <i>L. monocytogenes</i> strains, which exhibited high resistance to all tested antibiotics. Additionally, multidrug-resistant strains were identified, including a <i>S. aureus</i> strain with strong potential to be methicillin-resistant. Our findings underscore the importance of molecular methods for detecting specific pathogens on food-processing surfaces, highlighting the need for improved monitoring and control measures in restaurant kitchens.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143964314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}