Foodborne pathogens and disease最新文献

{"title":"An Investigation of an Outbreak of <i>Salmonella</i> Newport Infections Linked to Melons from Southwest Indiana-United States, 2023.","authors":"Natalie Cataldo, Colin Schwensohn, Margaret Kirchner, Erin Jenkins, Temesgen Jemaneh, Sharon Seelman, Manashi Dey, Casey Hamblin, Darcy Brillhart, Alex Goodman, Kurt Nolte, Adam Baker, Tim Jackson, Bob Literman, Rebecca L Bell, Tracy Hawkins, Laura Gieraltowski, Monique Salter, Stelios Viazis","doi":"10.1089/fpd.2024.0171","DOIUrl":"https://doi.org/10.1089/fpd.2024.0171","url":null,"abstract":"<p><p>The Food and Drug Administration (FDA), the Centers for Disease Control and Prevention (CDC), and state health and regulatory partners have investigated outbreaks of <i>Salmonella enterica</i> serovar Newport and Typhimurium infections in 2012, 2020, 2022, and 2023 linked to cantaloupes and/or watermelons from Southwest Indiana. In 2023, an outbreak of <i>Salmonella</i> Newport infections included 11 ill people and five hospitalizations reported in six states. The FDA and CDC collaborated with state partners to investigate the outbreak. The traceback investigation did not identify a single point of convergence for cantaloupe or watermelon. Products and environmental samples collected by the FDA and state partners from farms, public land, distributors, and points of service yielded isolates of multiple <i>Salmonella</i> serovars, including <i>Salmonella</i> Typhimurium and <i>Salmonella</i> Newport, that matched by whole genome sequencing to the 2020, 2022, and 2023 outbreak strains. Due to limitations with the traceback investigation and lack of convergence, a specific type of melon or source of contamination was not identified. However, the laboratory findings and historical outbreak information provided additional evidence to support a finding of ongoing contamination issues for cantaloupes and watermelons grown in Southwest Indiana. This is the fourth outbreak of salmonellosis confirmed to be linked to cantaloupes and/or watermelons from this area since 2012. These outbreaks of reoccurring and persisting strains of <i>Salmonella</i> illustrate the urgent need to determine the source, pathway, and extent of environmental contamination in the melon-growing region of Southwest Indiana and for outreach and education to help promote practices to reduce the contamination of melons.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Antibacterial Efficacy and Mechanism of Tea Polyphenol Against Drug-Resistant <i>Aeromonas veronii</i> TH0426 <i>In Vitro</i>.","authors":"Liying Peng, Zongtao Chen, Yanting Hei, Wenyan Wei, Defang Chen","doi":"10.1089/fpd.2024.0072","DOIUrl":"10.1089/fpd.2024.0072","url":null,"abstract":"<p><p>The emergence of Motile Aeromonas Septicemia (MAS) caused by <i>Aeromonas veronii</i> in sturgeon farming has become a significant concern due to its high mortality impact on the aquaculture industry. The threat posed by MAS highlights the urgent need for effective control measures to combat bacterial infections in sturgeon populations. Tea polyphenol (TP) has demonstrated promising antibacterial properties against livestock and poultry bacterial infections. However, its antibacterial efficacy and mechanism in bacterial diseases of aquatic animals remain largely unexplored. This study aimed to investigate the <i>in vitro</i> antibacterial effect and mechanism of TP on fish-borne drug-resistant <i>A. veronii</i> TH0426 by assessing the impact of TP on TH0426 cell growth, antibiofilm activity, morphology, as well as measuring electrical conductivity, DNA extravasation, lactate dehydrogenase (LDH) activity, protein, and DNA contents. Results demonstrated that the minimum inhibitory concentration and the minimum bactericidal concentration of TP on TH0426 were 1024 and 2048 μg/mL, respectively. After a 4 h treatment, the growth of TH0426 was completely inhibited at the concentration of 1024 and 2048 μg/mL of TP. Meanwhile, TP exhibited a significant antibiofilm activity. Both scanning electron microscope and transmission electron microscope analyses revealed disrupted cell membrane structure, irregular cell morphology, and loss of intracellular contents following TP treatment. Moreover, increased cell membrane permeability induced by TP led to intracellular ion and DNA leakage, resulting in elevated electrical conductivity and DNA extravasation. Furthermore, TP decreased LDH activity, protein concentration and content, DNA fluorescence intensity, and density in a time-dependent manner, indicating inhibition of protein metabolism and DNA synthesis. In conclusion, TP exhibits potent antibacterial properties by inhibiting biofilm formation, disrupting cell membrane integrity, and interfering with protein metabolism and DNA synthesis in drug-resistant <i>A. veronii</i> TH0426 <i>in vitro</i>.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"422-429"},"PeriodicalIF":1.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142389178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Antibacterial Effect of Lactic Acid Bacteria and Baicalin Against <i>Staphylococcus aureus In Vitro</i> and <i>In Vivo</i>.","authors":"Yan-Ni Mao, Yan-Jun Ma, Gui-Qin Wang","doi":"10.1089/fpd.2024.0086","DOIUrl":"10.1089/fpd.2024.0086","url":null,"abstract":"<p><p>Pathogenic bacteria such as <i>Staphylococcus aureus</i> (<i>S. aureus</i>) are the principal cause of cow mastitis, which primarily impacts milk yield and results in significant financial losses for the animal husbandry industry. Lactic acid bacteria-cell-free supernatant (LAB-CFS) and baicalin (BAI) both have a number of biological effects, including decreasing inflammation. The combined use of LAB-CFS and BAI does not appear to have been used to protective against mastitis, however, and the underlying mechanisms are yet unknown. In this study, <i>in vitro</i> activity of LAB-CFS and BAI alone and in combination was determined (checkerboard experiments, time-kill curves, and flow cytometry to investigate membrane permeability) and examined the protective effects of LAB-CFS and BAI on <i>S. aureus</i>-induced mastitis in mice and the impact of NF-κB signaling pathways on the emergence of mastitis. We discovered that when LAB-CFS and BAI were used together, <i>S. aureus</i> was more effectively treated than when LAB-CFS and BAI were used separately. Flow cytometry demonstrated that LAB-CFS and BAI work together to kill bacteria. <i>In vivo</i>, the usage of LAB-CFS and BAI decreased the activity of myeloperoxidase, as well as IL-6, IL-1β, and TNF-α secretion and the levels of TLR2 and p65 (NF-κB) expression. These findings suggested that LAB-CFS and BAI had a preventive effect against mastitis brought on by <i>S. aureus</i>. Therefore, the NF-κB signaling pathway is thought to be the likely mechanism through which LAB-CFS and BAI reduced <i>S. aureus</i>-induced inflammation in the mammary of cows. For the treatment of cow mastitis, LAB-CFS and BAI are likely to replace antibiotics.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"376-384"},"PeriodicalIF":1.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142616986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infection Status, Etiological Analysis of <i>Aeromonas</i> spp. in Foodborne Diarrhea Patients from 2019 to 2023 in Wenzhou.","authors":"Huihuang Lou, Yi Li, Yuejin Wu, Yuqin Hu, Leyi Zhang","doi":"10.1089/fpd.2024.0030","DOIUrl":"10.1089/fpd.2024.0030","url":null,"abstract":"<p><p>The infection status and etiological analysis of <i>Aeromonas</i> spp. from foodborne diarrhea patients in Wenzhou were carried out to provide the etiological basis for healthy diet and clinical treatment. <i>Aeromonas</i> isolates (<i>n</i> = 41) collected from foodborne diarrhea patients were identified using the automatic bacteriologic analyzer and mass spectrometer. Species identification, multilocus sequence typing, prediction of virulence genes, and antimicrobial resistance genes were analyzed by the data of whole genome sequencing. The antibiotic resistance of these isolates was determined using miniaturization of the broth dilution susceptibility test. A total of 1829 stool samples of diarrhea patients were collected, and the detection rate of <i>Aeromonas</i> spp. was 2.24% (41/1829). Moreover, <i>Aeromonas</i> spp. are more easily detected in warmer months (from June to August), which were identified as follows: <i>A. veronii</i> (53.66%, 22/41), <i>A. caviae</i> (21.95%, 9/41), <i>A. hydrophila</i> (9.76%, 4/41), <i>A. dhakensis</i> (4.88%, 2/41), <i>A. rivipollensis</i> (4.88%, 2/41), <i>A. enteropelogenes</i> (2.44%, 1/41), and <i>A. media</i> (2.44%, 1/41). All strains can be divided into 38 sequence types, 31 of which were novel, suggesting that <i>Aeromonas</i> spp. had high genetic diversity, multiple clones, and various sources in diarrhea patients. High number of genetic diversity and resistance were found in the <i>Aeromonas</i> isolates. In addition, the category distribution of the virulence genes was significantly different among the seven species of <i>Aeromonas</i>. <i>Aeromonas</i> spp. had different degrees of resistance to antibiotics, and tetracycline was the most serious, with a resistance rate of 27%. What's more, for some antimicrobial classes <i>in silico</i> antimicrobial resistance gene detection was highly correlated with phenotypic antimicrobial resistance patterns with an overall sensitivity of 93.3% and a specificity of 66.7%. The findings from this research highlighted the importance for development of prevention and control strategies to reduce the risk of foodborne diarrhea caused by <i>Aeromonas</i> spp.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"414-421"},"PeriodicalIF":1.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence and Characterization of the High-Level Tigecycline Resistance Gene <i>tet</i>(X4) in <i>Salmonella enterica</i> Serovar Rissen from Food in China.","authors":"Hongzhi Zhang, Wenjie Chen, Xiaoyu Lu, Yingying Liang, Xiao Quan, Xin Liu, Tianqi Shi, Ying Yu, Ruichao Li, Huanyu Wu","doi":"10.1089/fpd.2024.0101","DOIUrl":"10.1089/fpd.2024.0101","url":null,"abstract":"<p><p>The plasmid-mediated <i>tet</i>(X4) gene has exhibited a high-level resistance to tigecycline (TGC), which has raised concerns globally regarding antibiotic resistance. Although the widespread <i>tet</i>(X4) has been found widely in <i>Escherichia coli</i>, it is scarcely found in other Enterobacteriaceae. This study aimed to characterize a ST469 <i>Salmonella enterica</i> serovar Rissen (<i>S.</i> Rissen) isolate harboring <i>tet</i>(X4) from pork, which was identified and characterized via antimicrobial susceptibility testing, conjugation assays, plasmid curing testing, whole-genome sequencing, and bioinformatic analysis. Ten ST469 <i>S.</i> Rissen isolates of 223 <i>Salmonella</i> spp. isolates were isolated from food samples in China during 2021-2023. One of 10 <i>S</i>. Rissen isolates, SM2301, carrying <i>tet</i>(X4) conferred high-level resistance to TGC (minimum inhibitory concentration > 8 µg/mL). The <i>tet</i>(X4) could be conjugated into different recipients, including <i>E. coli</i>, <i>S. enteritidis</i>, and <i>K. pneumoniae</i> isolates. Plasmid curing confirmed that <i>tet</i>(X4) was plasmid-mediated. Genetic analysis revealed that the <i>tet</i>(X4) in the SM2301 isolate was located in the IncFIA(HI1)-IncHI1A-IncHI1B(R27) hybrid plasmid, and the structure of <i>tet</i>(X4) was <i>abh</i>-<i>tet</i>(X4)-IS<i>CR2</i>. To the best of our knowledge, this is the first report of a <i>tet</i>(X4)-positive food-derived <i>S. Rissen</i> isolate. The extending bacterial species of <i>tet</i>(X4)-bearing plasmids suggested the increasing transmission risk of the mobile TGC resistance gene <i>tet</i>(X4) beyond <i>E. coli</i>. This study highlights the emerging and evolution risk of novel resistance genes across various bacterial species. Therefore, further surveillance is warranted to monitor the prevalence of <i>tet</i>(X4) in <i>Salmonella</i> spp. and other bacterial species.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"405-413"},"PeriodicalIF":1.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142364982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory Activity and Mechanism of High-Voltage Prick Electric Field Against <i>Salmonella enteritidis</i> on Gelatin Plates and Selected Food Systems.","authors":"Haiyang Yan, Shuanglin Liu, Youhua Xian, Qingyan Liu, Yan Zhang, Yuan Yuan","doi":"10.1089/fpd.2024.0054","DOIUrl":"10.1089/fpd.2024.0054","url":null,"abstract":"<p><p>The present study assesses the antibacterial effect of a high-voltage prick electric field (HVPEF) on <i>Salmonella enteritidis</i> on gelatin plates and selected food systems. The results showed that <i>S. enteritidis</i> were susceptible to HVPEF treatment, with a mortality rate of 98% on the gelatin plate at 13 kV for 15 min. The survival rate of <i>S. enteritidis</i> decreased linearly with time and then stabilized after a sharp decline. The morphological change and the loss of internal nucleic acids and proteins suggest that the cell membrane was damaged by HVPEF, appearing more permeable and less fluid. Compared with the untreated group, both reactive oxygen species (ROS) and malondialdehyde increased significantly in the treated groups. Flow cytometry results suggest that DNA was severely damaged, which led to cell death, as shown by the elevated levels of intracellular ROS and lipid peroxidation. The death rate of <i>S. enteritidis</i> on the food surface significantly rose with the increase of HVPEF intensity, reaching 91 ± 1.6% (salmon), 88.2 ± 2.2% (chicken breast), 72.9 ± 2.7% (sausage), and 66.9 ± 1.6% (steak), respectively, at 14 kV for 30 min. HVPEF resulted in no significant change in color or texture; the changes in total viable count and pH were less significant than in the untreated group. Finally, HVPEF treatment led to lower levels of total volatile base nitrogen and thiobarbituric acid reactive substances in chicken breasts, which potentially contributes to longer shelf life.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"385-397"},"PeriodicalIF":1.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142282795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ten Years Later: Still Unchanged Susceptibility to Antibiotics of <i>Listeria monocytogenes</i> from Poultry Processing Environments.","authors":"Rakel Montiel, David Fernández, Pilar López, Sagrario Ortiz, David Pérez-Boto, Juan L Arqués, Joaquín V Martínez-Suárez","doi":"10.1089/fpd.2024.0102","DOIUrl":"10.1089/fpd.2024.0102","url":null,"abstract":"<p><p><i>Listeria monocytogenes</i> is still recognized as being commonly susceptible to antibiotics; however, there have been reports of reduced susceptibility in recent years. The significance of this resistance is not clear, in part due to the disparity in the antimicrobial susceptibility testing methods used. EUCAST (European Committee on Antimicrobial Susceptibility Testing) has recently proposed a standardized method for antibiotic susceptibility testing of <i>L. monocytogenes</i>. The aim of this work was to evaluate the susceptibility to 11 antibiotics in clinical use of 50 pulsed-field gel electrophoresis types of <i>L. monocytogenes</i> representing 347 isolates from a poultry industry setting using the EUCAST method and to compare the results with those obtained 10 years before. All poultry strains were sensitive to all the antibiotics tested but one strain was resistant to benzylpenicillin according to the EUCAST criteria. The current findings supported the previous study and confirmed that in certain food-associated <i>L. monocytogenes</i> populations, antibiotic sensitivity has remained stable.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"430-433"},"PeriodicalIF":1.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of <i>Staphylococcus</i> spp. and Coliform Bacteria Contamination Sources after Cleaning-in-Place in Production Lines of Dairy Factories in Türkiye.","authors":"Fulden Karadal, Nurhan Ertas Onmaz, Cemalettin Bagci, Yeliz Ucar Yildirim, Harun Hizlisoy, Zafer Gonulalan, Serhat Al","doi":"10.1089/fpd.2024.0103","DOIUrl":"10.1089/fpd.2024.0103","url":null,"abstract":"<p><p>Microorganisms detected on dairy factory surfaces after disinfection can cause product contamination, leading to economic losses and health hazards for consumers. In this study, the presence of <i>Staphylococcus</i> spp. and Coliform in a total of 450 samples taken from food-contact and non-contact surfaces (stainless steel, plastic, cloth, and tiles surfaces), raw milk and final product (white cheese, kashar cheese, butter, yogurt, and cream) samples in five dairy factories was investigated by conventional techniques. The isolates obtained were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. In this study, a total of 54 <i>Staphylococci</i> (16.7% <i>S. aureus</i> and 81.5% coagulase-negative <i>Staphylococci</i> [CNS]) and 44 coliform isolates were identified at the species level. The most common CNS isolated by samples was <i>S. epidermidis</i> followed by <i>S. saprophyticus, S. capitis, S. succinus S. carnosus</i>. <i>S. xylosus, S. sciuri, S. equorum, S. warneri,</i> and <i>S. hominis</i>. Eighteen of the coliform isolates (41%) were identified as <i>E. coli</i>; 13 (29.5%) as <i>E. cloacae</i>; 3 (6.9%) as <i>E. kobei, C. freundii,</i> and <i>K. oxytoca</i>; 2 (4.5%) as <i>K. pneumoniae</i>; 1 (2.3%) as <i>E. ludwigii</i> and <i>C. farmeri</i>. The contamination rate of non-food contact surfaces (71.3%) was found to be higher than food contact surfaces (10.4%), and contaminated surfaces were found to be effective in product contamination. Study results show that some bacterial species obtained from raw milk, surfaces, and final products are factory specific and surface-associated bacteria are prominent in the product microbial profile.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"398-404"},"PeriodicalIF":1.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retrospective Analysis of the Results of Five Consecutive Proficiency Tests Organized by the German National Reference Laboratory for <i>Listeria monocytogenes</i>.","authors":"Christoph-Martin Ufermann, Marlen Adler, Stefanie Lüth, Sascha Al Dahouk, Sylvia Kleta","doi":"10.1089/fpd.2024.0156","DOIUrl":"https://doi.org/10.1089/fpd.2024.0156","url":null,"abstract":"<p><p><i>Listeria</i> (<i>L</i>.) <i>monocytogenes</i> is an environmental pathogen that can contaminate a wide variety of food products, leading to foodborne listeriosis outbreaks. In this context, ready-to-eat (RTE) foods of animal and plant origin are of particular relevance as the source of infection. In Germany, the official food and veterinary control laboratories, under the sovereignty of the federal states, examine foodstuffs for the presence of foodborne pathogens, including <i>Listeria</i>. The German National Reference Laboratory for <i>L. monocytogenes</i> regularly organizes inter-laboratory proficiency tests (PTs) to monitor the accuracy and comparability of analyses performed by these official control laboratories. In our study, we provide summary data on frequently contaminated RTE food products in the European Union and present the outcome of five consecutive PTs. RTE food matrices provided by the German National Reference Laboratory for <i>L. monocytogenes</i> included processed meat, fish, vegetable, and dairy products. Overall, the official control laboratories achieved excellent results in the PTs. In total, 91 participants were documented. Flawless results were reported in 93.4% of the cases. Only four times, participants did not pass the PT in the first attempt. By performing official food and veterinary controls along the food chain, control laboratories make a significant contribution to food safety. To keep the excellent standard of the official control laboratories in Germany in line with future challenges in food safety, PTs with relevant RTE food matrices are indispensable.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Houseflies as Vectors of ESBL-Producing Enterobacterales: Insights from Hospital Settings in a Low- to Middle-Income Country.","authors":"Noah Obeng-Nkrumah, Paul Kwao, Enid Owusu, Gloria Della Tawiah-Abrokwa, Jerry Dzidzornu-Gomez, David Baidoo, Francisca Nana Afua Duah, Sarah Saidu, Appiah-Korang Labi","doi":"10.1089/fpd.2024.0099","DOIUrl":"https://doi.org/10.1089/fpd.2024.0099","url":null,"abstract":"<p><p>The spread of extended-spectrum beta-lactamase-producing Enterobacterales (ESBL-E) in communities has been facilitated by direct contact with humans or animal carriers and insects such as houseflies. Houseflies may have an increased risk of human exposure to antimicrobial-resistant pathogens, including ESBL-E. In this study, we determined the presence of ESBL-E in houseflies and ready-to-eat food samples from selected food vending sites in five health care facilities in Greater Accra, Ghana. Sixty (60) food vending sites were included in this study. Flies (10) were pooled, and food (5 g) was macerated and homogenized aseptically in brain-heart infusion agar. Suspensions were cultured on MacConkey agar supplemented with 4 µg/mL cefotaxime for ∼18 h. Phenotypic and molecular characterization of ESBL-E was performed using a combination disk diffusion method, multiplex polymerase chain reaction, and direct nucleotide sequencing. Of the 60 food vending sites, 62% (37) of the food vendors had flies within their premises that carried ESBL-E, and 15% (9) of the food samples collected from the vendors were contaminated with ESBL-E. The most predominant ESBL-producing Enterobacterales from housefly samples was <i>Proteus vulgaris</i> (54%) and that of food samples were <i>P. vulgaris</i> (22.5%) and <i>Escherichia coli</i> (22.5%). All Enterobacterales from flies and food samples harbored an ESBL gene with <i>bla_CTX-M</i>, mostly <i>bla_CTX-M-15</i>, being the predominant gene and gene type, respectively. Houseflies may be potential contributors to the spread of ESBL-producing Enterobacterales in Ghana.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}