Foodborne pathogens and disease最新文献_第3页

Molecular Detection and Genotyping of Blastocystis in Farmed Minks and Raccoon Dogs in Northern China. 中国北方养殖水貂和貉囊虫的分子检测和基因分型。

IF 1.9 2区农林科学

Foodborne pathogens and disease Pub Date : 2025-04-14 DOI: 10.1089/fpd.2024.0181

Nian-Yu Xue, Yanchun Wang, Hai-Tao Wang, Qing-Yu Hou, Ya Qin, Jing Jiang, Yan Tang

{"title":"Molecular Detection and Genotyping of Blastocystis in Farmed Minks and Raccoon Dogs in Northern China.","authors":"Nian-Yu Xue, Yanchun Wang, Hai-Tao Wang, Qing-Yu Hou, Ya Qin, Jing Jiang, Yan Tang","doi":"10.1089/fpd.2024.0181","DOIUrl":"https://doi.org/10.1089/fpd.2024.0181","url":null,"abstract":"Blastocystis is a common intestinal protozoan found in both humans and animals. However, research on Blastocystis infections in minks (Neovison vison) and raccoon dogs (Nyctereutes procyonoides) is limited. This study aimed to investigate the prevalence and subtypes of Blastocystis in these two species across northern provinces of China using small subunit rRNA gene sequence analysis. We examined 275 minks (89 from Hebei Province, 57 from Heilongjiang Province, 109 from Liaoning Province, and 20 from Shandong Province) and 235 raccoon dogs (114 from Hebei Province, 27 from Heilongjiang Province, 61 from Liaoning Province, and 33 from Jilin Province). The overall infection rate was 13.5% (69/510). Farmed minks had a prevalence of 1.5% (4/275), while raccoon dogs showed a significantly higher prevalence of 27.7% (65/235). We identified five distinct subtypes (ST1, ST2, ST3, ST5, and potentially novel subtype) across both species: ST1 (15 cases), ST2 (15 cases), ST3 (31 cases), ST5 (7 cases), and potentially novel subtype (1 case). This study is the first to report ST1 and ST3 in minks and ST1, ST2, ST5, and potentially novel subtype in raccoon dogs. As ST1, ST2, ST3, and ST5 subtypes are also found in humans, our findings suggest that minks and raccoon dogs may serve as potential reservoirs for human Blastocystis infections. Furthermore, the newly identified, potentially novel subtype might also be transmissible to humans or other animals. This research enhances our understanding of Blastocystis distribution among hosts in China and supports zoonotic disease prevention efforts related to farmed minks and raccoon dogs.","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143996155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protease Stabilizing Antimicrobial Peptide D1018M Showed Potent Antibiofilm and Anti-Intracellular Bacteria Activity Against MRSA. 蛋白酶稳定抗菌肽D1018M对MRSA具有有效的抗菌膜和胞内细菌活性。

IF 1.9 2区农林科学

Foodborne pathogens and disease Pub Date : 2025-04-14 DOI: 10.1089/fpd.2024.0134

Zirui Zhang, Jian Jiao, Jili Zhang, Lian Tan, Xunxi Dong, Runzhe Wu, Qiang Wang, Hao Wang, Xiao Wang

{"title":"Protease Stabilizing Antimicrobial Peptide D1018M Showed Potent Antibiofilm and Anti-Intracellular Bacteria Activity Against MRSA.","authors":"Zirui Zhang, Jian Jiao, Jili Zhang, Lian Tan, Xunxi Dong, Runzhe Wu, Qiang Wang, Hao Wang, Xiao Wang","doi":"10.1089/fpd.2024.0134","DOIUrl":"https://doi.org/10.1089/fpd.2024.0134","url":null,"abstract":"Methicillin-resistant Staphylococcus aureus (MRSA) poses a major threat to human health and food safety, especially when bacteria form biofilms or invade host cells, which may cause recurring infections. A new solution is therefore urgently needed. The antimicrobial peptide innate defense regulator (IDR)-1018 and its derived peptide 1018M showed promising antimicrobial and antibiofilm activities. Nevertheless, their antibacterial efficacy against intracellular MRSA and protease tolerance remains to be promoted. Therefore, we synthesized D-amino acid substitution peptides D1018 and D1018M. The antimicrobial activity against MRSA of these novel peptides was increased by 1-fold (D1018) or remained constant (D1018M) compared with L-amino acids peptides. Their bactericidal mechanisms involve cell wall destruction, membrane penetration, and genomic DNA disruption. As expected, the stability of D1018 and D1018M was increased by 2-32 times against pepsin, trypsin, and cathepsin K. In addition, by D-amino acids substitution, the antibiofilm ability of D1018 was increased by 1.6 times, and the anti-intracellular bacterial activity of D1018M was improved 3.2-5.7 orders of magnitude. These data indicated that D1018M is a potential antimicrobial candidate for recurring MRSA infections.","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143981904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application Research on the Triplex RT-ddPCR Assay for the Simultaneous Detection of Human Astrovirus and Sapovirus in Bivalve Shellfish. 三联RT-ddPCR法在双壳贝类中同时检测人类星状病毒和萨波病毒的应用研究。

IF 1.9 2区农林科学

Foodborne pathogens and disease Pub Date : 2025-04-14 DOI: 10.1089/fpd.2025.0011

Maolin Wei, Jinfeng Wang, Yan Wang, Libing Liu, Xiangdong Xu, Jianchang Wang

{"title":"Application Research on the Triplex RT-ddPCR Assay for the Simultaneous Detection of Human Astrovirus and Sapovirus in Bivalve Shellfish.","authors":"Maolin Wei, Jinfeng Wang, Yan Wang, Libing Liu, Xiangdong Xu, Jianchang Wang","doi":"10.1089/fpd.2025.0011","DOIUrl":"https://doi.org/10.1089/fpd.2025.0011","url":null,"abstract":"Human astrovirus (HAstV) and sapovirus (SaV) are significant pathogens associated with acute gastroenteritis in humans. This study established a triple reverse transcription-droplet digital polymerase chain reaction (PCR) (RT-ddPCR) assay incorporating MS2 phage as a process control virus for the simultaneous quantification of HAstV and SaV. The assay was validated using 240 bivalve samples, comprising five shellfish species: Ostreidae (n = 43), Ruditapes philippinarum (n = 84), Sinonovacula constricta (n = 27), Scapharca subcrenata (n = 23), and Pectinidae (n = 63). The results indicated that the developed RT-ddPCR assay had a good exclusivity, with detection limits of 5.15 copies/reaction for HAstV, 7.71 copies/reaction for SaV, and 6.13 copies/reaction for MS2 RNA. Viral screening revealed HAstV in 1.25% (3/240) of samples, with a maximum load of 22,140 copies/2 g, while SaV exhibited a higher prevalence of 13.33% (32/240) and a peak concentration of 68,700 copies/2 g. Different species of bivalve shellfish exhibited varying detection rates; the highest SaV detection rate was found in Ostreidaes at 20.93% (9/43), followed by Ruditapes philippinarums at 14.29% (12/84), Scapharca subcrenatas at 13.04% (3/23), Pectinidaes at 11.11% (7/63), and Sinonovacula constrictas at 3.70% (1/27). HAstV was only detected in R. philippinarums and Pectinidaes, with detection rates of 1.19% (1/84) and 3.17% (2/63), respectively. Additionally, both HAstV and SaV were detected in a single Pectinidae sample (0.42%, 1/240). The triple RT-ddPCR assay developed in this study is reliable, accurate, and highly sensitive, providing effective technical support for the quantitative detection of HAstV and SaV in bivalve shellfish.","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143990593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence and Abundance of Antibiotic Resistance Genes in Chinese Traditional Pickles. 中国传统腌菜耐药基因的发生与丰度。

IF 1.9 2区农林科学

Foodborne pathogens and disease Pub Date : 2025-04-14 DOI: 10.1089/fpd.2024.0190

Delong Li, Fumin Tan, YiXin Sun, Cuijuan Gao, Yunguo Liu

{"title":"Occurrence and Abundance of Antibiotic Resistance Genes in Chinese Traditional Pickles.","authors":"Delong Li, Fumin Tan, YiXin Sun, Cuijuan Gao, Yunguo Liu","doi":"10.1089/fpd.2024.0190","DOIUrl":"https://doi.org/10.1089/fpd.2024.0190","url":null,"abstract":"With the widespread application and even misuse of antibiotics, antibiotic resistance genes (ARGs) are extensively present in various environments, from natural environment to fermented foods, posing emerging threat to public and environmental health. The real-time fluorescence quantitative PCR (qPCR) technique is commonly used to detect ARGs of environmental samples such as soil or water. In this study, eight types of pickles were collected from four regions of China and the existence of 13 resistance genes was assessed by qPCR. The results showed that a total of 11 resistance genes were detected in pickles, the blaTEM gene was detected in all samples, and the neo and cat genes were absent. The abundance of resistance genes varied, aada1 (1.09 × 105 to 5.94 × 106 copies/g), blaTEM (1.48 × 105 to 2.2 × 106 copies/g), ermc (1.01 × 105 to 5.35 × 105 copies/g), hyg (1.35 × 105 to 1.93 × 106 copies/g), aadd (4.46 × 105 to 1.60 × 106 copies/g), nat1 (1.04 × 105 to 5.04 × 105 copies/g), nptII (2.17 × 104 to 1.69 × 105 copies/g), sul1 (2.01 × 105 to 4.60 × 105 copies/g), tetl (1.23 × 105 to 6.18 × 105 copies/g), shble (1.68 × 104 copies/g), and stra (4.8 × 104 to 1.9 × 105copies/g). We also discussed the specificity and sensitivity assessment of qPCR applied to ARGs analysis in pickles, verifying the feasibility and validity of the method. Bacteria were isolated and purified from pickles as well and their antimicrobial resistance was studied. This study is of great significance for the risk assessment of resistance genes in pickles. Effective and preventive solutions were proposed to reduce the spread of resistance genes and protect public dietary health.","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tracking Extended-spectrum β-lactamase-Producing and Colistin-Resistant Escherichia Coli in Pig Abattoirs: Impacts on Food Safety. 猪屠宰场产β-内酰胺酶和耐粘菌素大肠杆菌的广谱追踪：对食品安全的影响

IF 1.9 2区农林科学

Foodborne pathogens and disease Pub Date : 2025-04-14 DOI: 10.1089/fpd.2024.0178

Imporn Saenkankam, Prasert Apiwatsiri, Natthapong Supimon, Waree Niyomtham, David J Hampson, Nuvee Prapasarakul

{"title":"Tracking Extended-spectrum β-lactamase-Producing and Colistin-Resistant Escherichia Coli in Pig Abattoirs: Impacts on Food Safety.","authors":"Imporn Saenkankam, Prasert Apiwatsiri, Natthapong Supimon, Waree Niyomtham, David J Hampson, Nuvee Prapasarakul","doi":"10.1089/fpd.2024.0178","DOIUrl":"https://doi.org/10.1089/fpd.2024.0178","url":null,"abstract":"The ongoing increase in antimicrobial resistance (AMR) in Escherichia coli, particularly the emergence of extended-spectrum β-lactamase (ESBL)-producing and colistin-resistant strains in livestock, is a significant public health concern. The effectiveness of pig abattoir management, specifically through Hazard Analysis and Critical Control Points (HACCP) protocols, in reducing antimicrobial-resistant contamination continues to be scrutinized. This study investigated the prevalence, characteristics, and critical contamination points of ESBL-producing E. coli (ESBL-Ec) and colistin-resistant ESBL-Ec across the slaughtering processes in two pig abattoirs in Thailand-one operating under HACCP standards and the other certified solely under Good Manufacturing Practices. A higher prevalence of ESBL-Ec was found in the non-HACCP facility (67.98%) compared with the HACCP facility (52.04%), especially in pig carcasses. Skin and carcass washing in HACCP facilities effectively decreased bacterial contamination. Conversely, non-HACCP facilities should implement measures such as cleaning skin with disinfectants at the lairage, regularly monitoring and adjusting the final washing protocol, and strict sterilization of chopping plates to effectively control contamination points. Most ESBL-Ec isolates were multidrug-resistant and carried blaCTX-M group 1 or group 9 genes. Additionally, 12.6% of these isolates were resistant to colistin, with the mcr-1 gene predominantly identified. ST10 was the dominant clone of mcr-carrying ESBL-Ec across various slaughtering stages and sample types. These findings highlight the importance of implementing HACCP protocols to reduce contamination, enhance food safety, and mitigate public health risks. Ongoing AMR monitoring to find critical points along the slaughtering process is essential to reduce sources of AMR transmission to consumers.","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic Profiling and Virulence Characterization of Cronobacter sakazakii Strains Isolated from Powdered Spices and Instant Cereals in Nanning, China. 南宁粉状香料和速食谷物中阪崎克罗诺杆菌的基因组分析和毒力鉴定

IF 1.9 2区农林科学

Foodborne pathogens and disease Pub Date : 2025-04-11 DOI: 10.1089/fpd.2024.0180

Lili Zhang, Kunpeng Bu, Hui Yang, Pu Li, Zicong He, Tong Wu, Xiaoyu Li, Hao Nong, Shuang Wu, Jianqiu Qin, Hong Shu

{"title":"Genomic Profiling and Virulence Characterization of Cronobacter sakazakii Strains Isolated from Powdered Spices and Instant Cereals in Nanning, China.","authors":"Lili Zhang, Kunpeng Bu, Hui Yang, Pu Li, Zicong He, Tong Wu, Xiaoyu Li, Hao Nong, Shuang Wu, Jianqiu Qin, Hong Shu","doi":"10.1089/fpd.2024.0180","DOIUrl":"https://doi.org/10.1089/fpd.2024.0180","url":null,"abstract":"Conobacter sakazakii represents a significant foodborne pathogen threatening susceptible populations, yet its molecular characteristics in powdered spices and instant cereals remain poorly characterized. This investigation analyzed 45 retail samples (20 spices, 25 cereals) collected from Nanning, China, through pathogen isolation, whole genome sequencing, and bioinformatics profiling to elucidate virulence determinants, antimicrobial resistance patterns, and genetic features. The study revealed an overall contamination rate of 22.2% (10/45), with contamination rates of 25.0% in spices and 20.0% in cereals. Predominant serotype O1 accounted for 70.0% of isolates, while multilocus sequence typing identified seven sequence types, including novel ST1008. Genomic analysis demonstrated high genetic diversity (allelic differences: 57-2678) and revealed 42 virulence-associated genes (including flgA, ompA, and rpoS) alongside multidrug resistance patterns (notably to selected β-lactams). Crucially, mobile genetic element-mediated mcr-9 (conferring colistin resistance) and astA (encoding heat-stable enterotoxin) were detected, indicating significant transmission risks. This study provides the first documentation of concurrent virulence-resistance characteristics in C. sakazakii from Chinese retail powdered foods, highlighting the need for enhanced surveillance throughout low-moisture food supply chains to mitigate infection risks in vulnerable populations.","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143977790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovations in Bacteriophage Genome Engineering for Combating Multidrug-Resistant Bacterial Infections. 抗多药耐药细菌感染的噬菌体基因组工程创新。

IF 1.9 2区农林科学

Foodborne pathogens and disease Pub Date : 2025-04-11 DOI: 10.1089/fpd.2024.0194

Riska Ayu Febrianti, Erlia Narulita, Erma Sulistyaningsih, Hardian Susilo Addy

{"title":"Innovations in Bacteriophage Genome Engineering for Combating Multidrug-Resistant Bacterial Infections.","authors":"Riska Ayu Febrianti, Erlia Narulita, Erma Sulistyaningsih, Hardian Susilo Addy","doi":"10.1089/fpd.2024.0194","DOIUrl":"https://doi.org/10.1089/fpd.2024.0194","url":null,"abstract":"Bacteriophage engineering is a promising strategy to address multidrug-resistant (MDR) bacterial infections that pose significant challenges to public health due to the overuse of antibiotics. Bacteria can develop resistance mechanisms, such as receptor modification and activation of antiviral defense systems, which further complicates the application of phage therapy. Additionally, long-term phage therapy can result in the production of anti-phage antibodies, which may interfere with treatment. These factors require advanced engineering techniques to improve the efficacy of phages and expand their host range. Recent advances in genome engineering methods, including CRISPR/Cas9, homologous recombination, and other synthetic biology techniques, offer promising solutions to these challenges. By modifying receptor-binding proteins and using high-yield screening methods, researchers can create phages that are better equipped to target MDR bacteria effectively. Furthermore, understanding the intricate interactions between phages and their bacterial hosts is critical to guiding these engineering efforts. Future development perspectives lie in integrating these advanced engineering techniques into clinical practice, potentially putting bacteriophages at the forefront of fighting MDR bacterial infections.","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycotoxicological and Antimicrobial Efficiency of Curcuma longa. 姜黄的真菌毒理学及抗菌效果研究。

IF 1.9 2区农林科学

Foodborne pathogens and disease Pub Date : 2025-04-10 DOI: 10.1089/fpd.2024.0166

Helal F Al-Harthi, Ioan Pet, Eman El-Dawy, Youssuf A Gherbawy

{"title":"Mycotoxicological and Antimicrobial Efficiency of Curcuma longa.","authors":"Helal F Al-Harthi, Ioan Pet, Eman El-Dawy, Youssuf A Gherbawy","doi":"10.1089/fpd.2024.0166","DOIUrl":"https://doi.org/10.1089/fpd.2024.0166","url":null,"abstract":"Turmeric (Curcuma longa) is a spice widely used worldwide. It has been used in the treatment and prevention of diseases since ancient times. During harvesting and storage, turmeric is exposed to contamination by different fungi that are responsible for mycotoxins production. Mycobiota and mycotoxins associated with turmeric spices samples were screened in this study. Aspergillus was the dominant genus, it recorded with an average total counts of 4.76 log colony-forming unit/g, and appeared in all 40 tested samples. Molecular identification with internal transcribed spacer sequence was used to identify the species. The common species were Aspergillus flavus (with 80% frequency of occurrence) and Aspergillus niger (100%). Twelve and 20 samples were contaminated with total aflatoxins (AFs) and ochratoxins A (OTA) with concentrations 1.8-2.8 and 3.3-4.6 Parts Per Billioni (PPB), respectively. The search for suitable alternative antimicrobial drug resistance has increased, and this led researchers to explore the use of plant extract in the treatment of infections in both humans and animals. The antimicrobial activity of turmeric with two samples (sample no. 13 positive AFs and OTA, sample no. 2 with no toxins) on selected Candida albicans, Escherichia coli, and Staphylococcus aureus was evaluated. Different types of turmeric extracts were prepared using three solvents namely water, methanol, and ethanol using the disc diffusion method. The two tested turmeric samples extracts showed inhibition activity against all tested microorganisms. The zones of inhibition exhibited by extract of turmeric in methanol solvent from sample no. 13 against test organisms more effective and ranged from 10.6 to 15.4 mm. This is due to the confusion between antimicrobial activity of extract and its positively to mycotoxins content.","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryptosporidium Spp. in Mink, Foxes, and Raccoon Dogs in Five Provinces of Northern China. 中国北方五省水貂、狐狸和貉的隐孢子虫属。

IF 1.9 2区农林科学

Foodborne pathogens and disease Pub Date : 2025-04-10 DOI: 10.1089/fpd.2025.0023

Zhen-Qiu Gao, Hai-Tao Wang, Nian-Yu Xue, Zhong-Yuan Li, Ya Qin, Xue-Min Li, Qing-Yu Hou, Jing Jiang, Li-Hua Yang, Xing Yang, Hong-Bo Ni, Yan Tang, Shuo Liu

{"title":"Cryptosporidium Spp. in Mink, Foxes, and Raccoon Dogs in Five Provinces of Northern China.","authors":"Zhen-Qiu Gao, Hai-Tao Wang, Nian-Yu Xue, Zhong-Yuan Li, Ya Qin, Xue-Min Li, Qing-Yu Hou, Jing Jiang, Li-Hua Yang, Xing Yang, Hong-Bo Ni, Yan Tang, Shuo Liu","doi":"10.1089/fpd.2025.0023","DOIUrl":"https://doi.org/10.1089/fpd.2025.0023","url":null,"abstract":"Cryptosporidium spp. is a common intestinal pathogen that causes diarrhea in both humans and animals. Northern China has a large-scale fur animal farming industry. Although there have been related studies, systematic data remain lacking. In this study, a total of 871 fecal samples were collected from fur animals in Shandong, Hebei, Liaoning, Jilin, and Heilongjiang provinces. Using nested polymerase chain reaction, 75 Cryptosporidium-positive samples were detected, and the overall infection rate was 8.61%. The infection rates ranged from 4.23% to 16.81% across different provinces, with the highest rate was observed in Shandong (16.81%, 19/113), followed by Jilin (15.07%, 11/73). Additionally, foxes (15.06%, 53/352) showed a higher infection rate compared with mink (4.55%, 13/286) and raccoon dogs (3.86%, 9/233), suggesting that foxes may be more susceptible. Four species/genotypes were identified: Cryptosporidium canis (n = 66), Cryptosporidium meleagridis (n = 1), Cryptosporidium parvum (n = 1), and mink genotype (n = 7). Among these, C. parvum and C. meleagridis were found in raccoon dogs and foxes, respectively, while the mink genotype was detected only in mink. C. canis was present in all three animal species and was the dominant species in foxes. These findings indicate that farmed fur animals may serve as potential reservoirs for Cryptosporidium transmission to humans. This study provides essential epidemiological data on Cryptosporidium infections in fur animals, contributing to a better understanding of its distribution and potential zoonotic risk.","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Hybrid Prediction Model Based on Decomposition-Integration for Foodborne Disease Risks. 基于分解-积分的食源性疾病风险混合预测模型

IF 1.9 2区农林科学

Foodborne pathogens and disease Pub Date : 2025-04-10 DOI: 10.1089/fpd.2024.0189

Ke Qin, Jingxiang Zhang, Xiaoting Dai, Linhai Wu, Minguo Gao

{"title":"A Hybrid Prediction Model Based on Decomposition-Integration for Foodborne Disease Risks.","authors":"Ke Qin, Jingxiang Zhang, Xiaoting Dai, Linhai Wu, Minguo Gao","doi":"10.1089/fpd.2024.0189","DOIUrl":"https://doi.org/10.1089/fpd.2024.0189","url":null,"abstract":"Foodborne diseases (FBDs) are contagious, explosive, clustered diseases caused by the ingestion of contaminated foods, which represent huge economic and health burdens globally. Reliably predicting the risk trend of FBDs has become a major challenge in the field of public health. This study aimed to design a risk prediction model suitable for predicting FBD risks by using the decomposition-integration technique. A total of 28,646 FBD cases from FBD surveillance data reported by all sentinel hospitals in Wuxi from 2019 to 2023 were included in the study. The obtained FBD risk data were decomposed into multiple intrinsic mode functions (IMFs) using complete ensemble empirical mode decomposition with adaptive noise, which were then reconstructed by calculating the sample entropy. Finally, the time dependence of the reconstructed IMFs was explored using a temporal convolution network-long short-term memory (TCN-LSTM) model to obtain the prediction results of each component, which were then linearly added to obtain the final prediction results. Compared with other models, our proposed prediction model significantly improved the prediction accuracy of FBD risks, with a best average root mean square error of 5.349 and mean absolute error of 3.819, demonstrating at least a 40% improvement in accuracy over standalone LSTM. The FBD risk prediction results obtained by the method proposed in this study can provide data support for food safety management and policy making and enable more accurate early warning of FBDs.","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0