Foodborne pathogens and disease最新文献

{"title":"Phenotypic and Whole-Genome Sequencing-Based Profiling of Antimicrobial Resistance and Virulence in <i>Campylobacter</i> spp. Isolated from Human Stool in a Croatian University Hospital.","authors":"Luka Katic, Ivana Ferenčak, Ana Gverić-Grginić, Ivana Goic-Barisic, Zana Rubic, Marina Radic-Skelin, Jelena Marinovic, Dora Smolić, Josipa Kuzle, Lucija Škara Abramović, Irena Tabain, Dragan Jurić, Marija Tonkic, Georgina Osorio, Anita Novak","doi":"10.1089/fpd.2024.0173","DOIUrl":"https://doi.org/10.1089/fpd.2024.0173","url":null,"abstract":"<p><p><i>Campylobacter</i> spp. is a leading cause of community-acquired gastroenteritis, with rising antimicrobial resistance (AMR) posing a significant public health challenge. This study aimed to characterize the genomic basis of resistance and virulence in <i>Campylobacter</i> spp. isolated from human stool at the University Hospital of Split, Croatia. A total of 115 unduplicated isolates (100 <i>Campylobacter jejuni</i> and 15 <i>Campylobacter coli</i>) were identified and tested for antimicrobial susceptibility, from January to June 2023. A representative number of isolates with phenotypically detected resistance were analyzed with whole-genome sequencing. Multilocus sequence typing (MLST) was determined using genomic sequences as input data against the standard MLST schemes. High rates of resistance were detected to tetracycline (45% of <i>C. jejuni</i> and 26.7% of <i>C. coli</i>) and ciprofloxacin (81% of <i>C. jejuni</i> and 46.7% of <i>C. coli</i>), while combined ciprofloxacin/tetracycline resistance was observed in 43% of <i>C. jejuni</i> and 7% of <i>C. coli</i> isolates. The majority of whole-genome sequenced isolates possess <i>tet(O/32/O)</i> gene and <i>gyrA.T861</i> mutation, conferring resistance to tetracycline and fluoroquinolones. All isolates have at least one resistance gene for β-lactams (<i>bla</i>_OXA-61, <i>bla</i>_OXA-193, <i>bla</i>_OXA-450, <i>bla</i>_OXA-451, <i>bla</i>_OXA-452, <i>bla</i>_OXA-461, and <i>bla</i>_OXA-489), while 40% of isolates possess <i>ant(6)-Ia</i> for aminoglycoside resistance. In addition, a newly emerged, phenotypically identified multidrug-resistant <i>C. coli</i>, harbored <i>tet(O/32/O)</i> and <i>tet(O)</i> genes, point mutation in <i>gyrA</i> gene, and A2075G mutation in 23S rRNA. Virulence analysis highlighted <i>C. jejuni</i>'s broad pathogenic potential, including motility, adherence, invasion, exotoxin production, and immune modulation (e.g., <i>wlaN</i> gene, linked to Guillain-Barré syndrome). On the contrary, <i>C. coli</i> isolates possess genes exclusively for motility. Nine different <i>C. jejuni</i> MLST sequence types were identified, while all <i>C. coli</i> isolates belong to the same 828 clonal complex. Escalating AMR and a broad spectrum of virulence in <i>Campylobacter</i> spp. highlight the importance of continuous surveillance on the phenotypic and genotypic level, thereby allowing more efficient health care management of these infections.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Antiparasitic Effects of Nitrate and Lactic Acid on <i>Sarcocystis</i> Bradyzoites in Meat: Enhancing Food Safety.","authors":"Mortaza Rahimi, Nasser Hajipour, Parviz Hassanzadeh","doi":"10.1089/fpd.2024.0174","DOIUrl":"https://doi.org/10.1089/fpd.2024.0174","url":null,"abstract":"<p><p><i>Sarcocystis</i> spp. are zoonotic protozoan parasites found worldwide, transmitted through the consumption of infected meat. These parasites form cysts in the muscles of livestock, causing economic losses and health risks, including reduced meat and milk production, abortions, and, in severe cases, death in intermediate hosts. This study evaluates the antiparasitic effects of nitrate and lactic acid against <i>Sarcocystis</i> bradyzoites in sheep meat, aiming to identify effective decontamination methods to enhance food safety. Samples of <i>Sarcocystis</i> cysts were collected from infected sheep organs and treated with various concentrations (3%, 6%, 9%, and 12%) of nitrate and lactic acid for 3-48 h. Bradyzoite viability was assessed microscopically after staining and digestion. Additionally, bioassays were conducted using cats to confirm the treatment's efficacy. Results demonstrated that both compounds significantly increased bradyzoite mortality with higher concentrations and longer exposure times. Lactic acid showed greater efficacy at lower concentrations; 100% mortality was achieved at 3% lactic acid after 48 h and 6% after 24 h. In contrast, nitrate required 12% concentration for 100% mortality within 24 h. Statistical analysis revealed a significant correlation between compound concentration and exposure time with bradyzoite mortality, highlighting lactic acid's superior antiparasitic properties at lower concentrations compared with nitrate. This study highlights the potential of lactic acid and nitrate as natural decontamination agents for meat safety. Their application could mitigate <i>Sarcocystis</i>-related risks, reduce foodborne parasitic infections, and contribute to the economic sustainability of meat production.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Trends of <i>Vibrio cholerae:</i> Global and Regional Incidences.","authors":"Beenzu Siamalube, Emmanuel Ehinmitan, Steven Runo, Justus Onguso, Maina Ngotho","doi":"10.1089/fpd.2024.0165","DOIUrl":"https://doi.org/10.1089/fpd.2024.0165","url":null,"abstract":"","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Differentially Expressed Proteins in Bovine Mammary Glands Infected with <i>Staphylococcus aureus</i>.","authors":"Li Zhou, Zhuo-Ma Luoreng, Xing-Ping Wang","doi":"10.1089/fpd.2024.0179","DOIUrl":"https://doi.org/10.1089/fpd.2024.0179","url":null,"abstract":"<p><p>Mastitis is among the most prevalent diseases in dairy cows, leading to substantial issues such as decreased milk yield and quality, reduced reproductive performance, early culling, and increased production costs. <i>Staphylococcus aureus</i>, a naturally occurring opportunistic pathogen, is majorly responsible for mastitis in dairy cows. Mammary tissues from healthy cows were used as a control group (M_C, <i>n</i> = 3), and mammary glands of Chinese Holstein cows infected with <i>S. aureus</i> (10⁵ colony-forming unit/mL) were used as experimental groups (M_S, <i>n</i> = 3). Histopathological examination and analysis of inflammatory factor expression confirmed the successful establishment of the mastitis model. Using label-free quantitative proteomics, we analyzed the protein expression profiles of normal healthy mammary tissues (M_C) and <i>S. aureus</i>-infected mammary tissues (M_S). The analysis identified 933 differentially expressed proteins in <i>S. aureus</i>-infected mammary tissues, with 608 upregulated and 325 downregulated proteins, compared with the healthy tissues. Gene ontology functional annotation indicated that these proteins are involved in biological processes such as oxidation-reduction, the extracellular region, and catalytic activity. The Kyoto Encyclopedia of Genes and Genomes enrichment unveiled that these proteins were associated with pathways including <i>S. aureus</i> infection, cell adhesion molecules, antigen processing, and presentation. According to the InterPro enrichment analysis, the immunoglobulin-like fold and pleckstrin homology domain were the most enriched structural domains. Integrated transcriptomic and proteomic analyses revealed that ETNK1 and VNN1 exhibited consistent expression trends at both the mRNA and protein levels, suggesting their potential roles as key regulators in the pathogenesis of mastitis. The results not only enhance our understanding of mastitis management at the protein level but also point the way to potential antimastitis targets.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and Identification of <i>Vibrio cholerae</i> Bacteriophages VC3 and Its Role in the Inhibition and Removal of Biofilms in Seafood.","authors":"Qingfang Hao, Yue Bai, Xiuli Bao, Siyuan Wang, Yue Hao, Ruxue Shao, Xinxin Kang, Lei Zhang, Mingsheng Lyu, Shujun Wang","doi":"10.1089/fpd.2024.0106","DOIUrl":"https://doi.org/10.1089/fpd.2024.0106","url":null,"abstract":"<p><p>As biological control agents, bacteriophages can both inhibit the pathogenic bacteria and remove the bacterial biofilms from the seafood. <i>Vibrio cholerae</i> is the pathogen of cholera and the severe infection could lead watery diarrhea and even death. The double-layer agar plate method was used to isolate and screen the <i>V. cholerae</i> bacteriophages from the samples of aquaculture water and sewage. Purified bacteriophages were examined through genome sequencing, as well as morphological and biological characterizations. Among the isolated bacteriophages, bacteriophage VC3 was found to be a long-tailed bacteriophage. Whole-genome sequencing showed that the full length of VC3 genome was 27,645 bp. It was a circular dsDNA, with 40.37% G + C content. The optimal multiplicity of infection was 1, the incubation period was 20 min, the burst period was 40 min, and the lysis volume was 73 PFU/cell. Bacteriophage VC3 exhibited good activity under low temperatures and neutral pH conditions. Bacteriophage VC3 could effectively inhibit and eliminate the biofilm of <i>V. cholerae.</i> In addition, bacteriophage VC3 significantly inhibited <i>V. cholerae</i> in fish fillets and shrimp meat. At the same time, it also showed lytic activity against 9 pathogenic bacteria, indicating that it has the potential to inhibit a variety of pathogenic bacteria.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Minimum Growth Temperatures of Shiga Toxin-Producing <i>Escherichia coli</i>.","authors":"Lin Walker, Shengqian Sun, Harshavardhan Thippareddi","doi":"10.1089/fpd.2024.0108","DOIUrl":"https://doi.org/10.1089/fpd.2024.0108","url":null,"abstract":"<p><p>The objective of this study was to determine the minimum growth temperature of Shiga toxin-producing <i>Escherichia coli</i> (STEC). Forty-eight strains of STEC, including <i>E. coli</i> O157:H7, O104:H4, O26, O45, O103, O111, O121, and O145, were inoculated into tryptic soy broth (TSB) at ca. 6.0 CFU/mL and incubated at temperatures ranging from 5°C to 11°C. The lowest temperature at which growth occurred was determined as the minimum growth temperature of the strain. The minimum growth temperature varied among strains, but the strain difference was within 2-3°C. All of the STEC strains grew at ≥10.3°C. Majority of the STEC strains (31/48) grew at 8.9°C, with some strains (10/48) being able to grow at as low as 8.0°C. None of the STEC serogroups were able to grow at ≤7.4°C. <i>E. coli</i> O104:H4 and O157:H7 had relatively lower minimum growth temperatures, with 8°C and average 8.4°C, respectively, whereas serogroup O26 had a higher minimum growth temperature (average 9.6°C). The results of this study provide basic but critical information on STEC growth and could be used either in fundamental research or to mitigate the risk of STEC from food products by storing them at temperatures below the minimal growth temperature.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143604309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a Colorimetric and Fluorescence Dual-Mode Immunoassay Detection of Alpha-Hemolysin in Milk.","authors":"Xu Xu, Pengfei Zhang, Fuqian Ruan, Guanhong Chang, Ting Zhou, DiShi Chen, Li Li, Xin Wang","doi":"10.1089/fpd.2023.0106","DOIUrl":"10.1089/fpd.2023.0106","url":null,"abstract":"<p><p>Alpha-hemolysin (Hla) is a major virulence factor secreted by <i>Staphylococcus aureus (S. aureus)</i>, which can lyse a variety of mammalian cells and help bacteria evade the host immune system or antibiotics, posing a safety hazard to human health. Therefore, it is critical to establish a quick-responsive and sensitive method for Hla detection to ensure food safety. In this work, a dual-mode immunoassay was developed with both colorimetric and fluorescent readouts for discriminative detection of Hla. The proposed sensing system consists of p-phenylenediamine (PPD) and fluorescein, where fluorescein functions as a fluorescent reporter, and PPD serves a dual function as a colorimetric reporter and fluorescence quencher. Subsequently, the reaction system of this method was optimized, and the detection limit, sensitivity, and specificity were evaluated. Under optimal conditions, the proposed method possesses excellent analytical performance in the range from 0.5 to 500 ng/mL with a limit of detection as low as 0.5 ng/mL. Noteworthy, this method was successfully employed for the detection of Hla in milk with good selectivity and high accuracy. Overall, the dual-mode immunoassay provides a superior platform for the on-site, quantitative, and accurate detection of Hla in food samples.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"167-176"},"PeriodicalIF":1.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140119227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Application of Four Foodborne Pathogens by TaqMan Multiplex Real-Time PCR.","authors":"Yinlei Xue, Shengfang He, Meng Li, Yuanhao Qiu","doi":"10.1089/fpd.2023.0134","DOIUrl":"10.1089/fpd.2023.0134","url":null,"abstract":"<p><p>A TaqMan multiplex real-time PCR (mRT-PCR) was developed to detect simultaneously <i>Salmonella</i> spp., <i>Escherichia coli</i> O157, <i>Staphylococcus aureus</i>, and <i>Listeria monocytogene</i>s in food samples. The method involves four sets of primers and probes tailored to the unique DNA sequences found in the <i>invA</i>, <i>nuc</i>, <i>rfbE</i>, and <i>hly</i> genes of each pathogen. The generated standard curves, correlating gene copy numbers with Ct values, demonstrated high accuracy (<i>R</i>² > 0.99) and efficiency (92%-104%). Meanwhile, the limit of detection was 10⁰ CFU/mL for the four target bacteria in artificially contaminated food samples after 6-8 h of enrichment. The assay's effectiveness was further verified by testing 80 naturally contaminated food samples, showing results largely in agreement with traditional culture methods. Overall, this newly developed TaqMan mRT-PCR, inclusive of a pre-enrichment step, proves to be a dependable and effective tool for detecting single or multiple pathogens in diverse food items, offering significant potential for <i>in vitro</i> diagnostics.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"193-201"},"PeriodicalIF":1.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deletion of Nox from <i>Listeria monocytogenes</i> Strain EGDe Enhances Bacterial Virulence and Reduces the Production of Reactive Oxygen Species and Inflammatory Factors <i>In Vivo</i>.","authors":"Dezhi Li, Wenwen Ma, Guowei Chen, Zhiqiang Huang, Qing Liu","doi":"10.1089/fpd.2023.0125","DOIUrl":"10.1089/fpd.2023.0125","url":null,"abstract":"<p><p>The foodborne pathogens have a serious threat to human health, especially <i>Listeria monocytogenes</i>. NADPH oxidase (NOX) is involved in cellular respiration and the production of reactive oxygen species (ROS), acting as messengers to host cells during the infection. However, the role of <i>nox</i> in the process of <i>L. monocytogenes</i> infection is unclear. In this study, we examined the impact of <i>nox</i> in <i>L. monocytogenes</i> by gene deletion. The results of cell experiment showed that knocking out <i>nox</i> from <i>L. monocytogenes</i> strain EGDe resulted in a twofold increase invasion ability to Caco-2 cells compared with that of wild-type strain (WT), but did not affect adhesion ability. Animal infection assays also showed that bacterial loads in the liver and spleen of mice challenged with EGDe-Δ<i>nox</i> were approximately two times higher compared with those challenged with the WT strain. On the one hand, quantitative real-time polymerase chain reaction revealed that deletion of <i>nox</i> leads to upregulation of genes related to the internalization of <i>L. monocytogenes</i> (<i>inlA</i>, <i>inlB</i>, and <i>inlC</i>). More importantly, the expression of listeriolysin-positive regulatory (<i>prfA</i>) gene increased by three times <i>in vivo</i> compared with that of WT. On the other hand, the deletion of <i>nox</i> resulted in a reduction of the upregulation of proinflammatory factors in EGDe-Δ<i>nox</i> compared with the WT and complementary strains. Thus, our study revealed that <i>nox</i> affected the virulence of <i>L. monocytogenes</i> by upregulating the expression of virulence genes and regulating the production of ROS and inflammatory factors <i>in vivo</i>.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"177-186"},"PeriodicalIF":1.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140131127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and Optimization of a <i>yst</i>-PCR to Detect <i>Yersinia enterocolitica</i> in Meat Food.","authors":"Anna C Mastrodonato, Walter Lapadula, Maximiliano Juri-Ayub, María E Escudero, Gabriela I Favier, Cecilia S M Lucero-Estrada","doi":"10.1089/fpd.2023.0126","DOIUrl":"10.1089/fpd.2023.0126","url":null,"abstract":"<p><p>In this study, a polymerase chain reaction (PCR) directed to the <i>yst</i> chromosomal gene (<i>yst</i>-PCR) was used as a rapid, sensitive, and specific method to detect <i>Yersinia enterocolitica</i> strains belonging to different biotypes in foods; a competitive Internal Amplification Control (cIAC) is also developed. The cIAC had a molecular weight of 417 bp and was detected until a concentration of 0.85 ng/μL. No other strains of other <i>Yersinia</i> species, nor <i>Enterobacteriales</i> order were detected by this PCR. In pure culture, the detection limit (DL) of the <i>yst</i>-PCR was lower for <i>ystA</i>⁺ strain (10 colony-forming unit [CFU]/mL) than for <i>ystB</i>⁺ strain (1 × 10² CFU/mL); which was the concentration detected in <i>Y. enterocolitica</i> inoculated minced meat. The proposed protocol included an enrichment step in peptone sorbitol bile (PSB) broth at 25°C for 24 h followed by isolation on Mac Conkey agar and chromogenic medium. An aliquot of the PSB broth homogenate and a loopful from the confluent zone of solid media were collected to perform DNA extraction for <i>yst</i>-PCR, and typical colonies were characterized by biochemical assays. Among 30 non-contaminated food samples, 4 samples were <i>yst-</i>positive and no <i>Y. enterocolitica</i> isolates were obtained. It is suggested that this <i>yst</i>-PCR could be used in the investigation of <i>Y. enterocolitica</i> in foods.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"187-192"},"PeriodicalIF":1.9,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140049168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}