Foodborne pathogens and disease最新文献

{"title":"Prediction of Antibiotic Resistance Phenotypes and Minimum Inhibitory Concentrations in <i>Salmonella</i> Using Machine Learning Analysis of Its Pan-Genome and Pan-Resistome Features.","authors":"Yichen He, Xiujuan Zhou, Lida Zhang, Yan Cui, Yiping He, Andrew Gehring, Xiangyu Deng, Xianming Shi","doi":"10.1089/fpd.2024.0170","DOIUrl":"https://doi.org/10.1089/fpd.2024.0170","url":null,"abstract":"<p><p>Traditional experimental methods for determining antibiotic resistance phenotypes (ARPs) and minimum inhibitory concentrations (MICs) in bacteria are laborious and time consuming. This study aims to explore the potential of whole-genome sequencing data combined with machine learning models for robustly predicting ARPs and MICs in <i>Salmonella</i>. Using a training set of 6394 <i>Salmonella</i> genomes alongside antimicrobial susceptibility testing results, we built two machine learning (ML) predictive models based on the pan-genome and pan-resistome. Each model was implemented using three algorithms: random forest, extreme gradient boosting (XGB), and convolutional neural network. Among them, XGB achieved the highest overall accuracy, with the pan-genome and pan-resistome models accurately predicting ARPs (98.51% and 97.77%) and MICs (81.42% and 78.99%) for 15 commonly used antibiotics. Feature extraction from pan-genome and pan-resistome data effectively reduced computational complexity and significantly decreased computation time. Notably, fewer than 10 key genomic features, often linked to known resistance or mobile genes, were sufficient for robust predictions for each antibiotic. This study also identified challenges, including imbalanced resistance classes and imprecise MIC measurements, which impacted prediction accuracy. These findings highlight the importance of using multiple evaluation metrics to assess model performance comprehensively. Overall, our findings demonstrated that ML, utilizing pan-genome or pan-resistome features, was highly effective in predicting antibiotic resistance and identifying correlated genetic features in <i>Salmonella</i>. This approach holds great potential to supplement conventional culture-based methods for routine surveillance of antibiotic-resistant bacteria.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NutriClay^Zn Harbors Bioavailable Zinc and Suppresses Multidrug-Resistant <i>Salmonella</i> Choleraesuis.","authors":"Steven J T Jackson, Kathleen Andrews, Robert E Droleskey, William J Banz, Gary A Apgar, Kelly J Rivenbark, Meichen Wang, Robin C Anderson, Roger B Harvey, Timothy D Phillips","doi":"10.1089/fpd.2025.0016","DOIUrl":"https://doi.org/10.1089/fpd.2025.0016","url":null,"abstract":"<p><p>Pharmacological doses of zinc oxide (ZnO), far in excess of the nutritional requirement for zinc, are commonly added to weanling swine feed to suppress enterotoxigenic bacteria and thereby support piglet weight gains. However, excessive ZnO in the diet has come under scrutiny due to concerns that excreted zinc may accumulate beyond safe levels within topsoil and water supplies, as well as foster antibiotic drug resistance in bacterial pathogens that could then infect livestock and/or humans. Indeed, multidrug-resistant (MDR) <i>Salmonella</i> have been isolated from swine feces, focusing attention on new technologies to protect swine (and ultimately humans) while reducing dietary zinc toward the nutritional requirement. We hypothesized that NutriClay^Zn (an edible Generally Recognized As Safe substance consisting of low-dose ZnO immobilized on montmorillonite clay) could suppress MDR <i>Salmonella</i>, provide bioavailable zinc, and serve as a novel feed ingredient for weanling swine. NutriClay^Zn dose-response efficacy and thermal stability were assessed in cultures of MDR <i>Salmonella</i> Choleraesuis. Dissociated elemental zinc was measured under simulated gastrointestinal conditions. NutriClay^Zn consumption was assessed via <i>ad libitum</i> feeding, with bioavailable zinc calculations according to weanling swine treatment group intakes. <i>Salmonella</i> Choleraesuis (resistant to quinolone, sulfonamide, and aminoglycoside antibiotics) was susceptible to growth inhibition by heat-stable NutriClay^Zn. Under simulated gastrointestinal conditions, 22.8 µg of elemental zinc were released per milligram NutriClay^Zn. Furthermore, the rate at which weanling swine consumed NutriClay^Zn-containing feed (0.6 kg/day) supports fulfillment of the nutritional zinc requirement. These findings encourage future MDR <i>Salmonella</i> pathogen challenge trials, designed with NutriClay^Zn replacing pharmacological ZnO for control of enterotoxigenic bacteria in swine, as well as potentially eliminating the zinc component of mineral premixtures that are currently added to feed for nutritional purposes.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycobiota and Their Mycotoxins in Egyptian Dried Figs.","authors":"Helal F Al-Harthi, Hanan AlOmari, Ioan Pet, Youssuf A Gherbaw","doi":"10.1089/fpd.2025.0048","DOIUrl":"https://doi.org/10.1089/fpd.2025.0048","url":null,"abstract":"<p><p>This study evaluates the impact of traditional sun drying and modern industrial drying techniques on fungal contamination and mycotoxin production in dried figs. A total of 80 samples (40 per drying method), collected from various retail sources in Upper Egypt, were analyzed. Fungal isolation was performed on Dichloran Rose Bengal Chloramphenicol agar medium and incubated at 28°C. Sun-dried figs exhibited significantly higher fungal loads (1395 colony-forming unit [CFU]/g) compared with industrially dried figs (750 CFU/g). Mycobiota analysis identified 33 fungal species across 12 genera in sun-dried figs, whereas industrial drying yielded 21 species. Internal transcribed spacer sequencing facilitated species identification, with accession numbers PV065865 to PV065896 deposited in GenBank. <i>Aspergillus</i> spp. were dominant in both drying methods, with <i>Aspergillus welwitschiae</i>, <i>A. flavus</i>, and <i>A. niger</i> being the most prevalent. Mycotoxin analysis revealed aflatoxin contamination in 37.5% of sun-dried and 15% of industrially dried figs, while ochratoxin A was detected in 57.5% and 27.5% of samples, respectively, for sun-dried and industrially dried figs. Total fumonisins were present in 12.5% of sun-dried and 5% of industrially dried figs. These findings highlight the efficacy of industrial drying techniques in mitigating fungal contamination and mycotoxin accumulation, thereby improving the microbiological safety of dried figs.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144274614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted Antimicrobial Resistance Gene Screening from Metagenomic DNA of Raw Milk Samples Identifies the Presence of Multiple Genes Including the <i>mcr9</i>.","authors":"Remina M Shajahan, Athul M, Farhana T A, Sreejith Sreekumaran, Divya Lakshmanan, Mithilesh Jaiswal, Radhakrishnan E K","doi":"10.1089/fpd.2024.0187","DOIUrl":"https://doi.org/10.1089/fpd.2024.0187","url":null,"abstract":"<p><p>The current study has investigated the prevalence of antimicrobial resistance (AMR) genes in cow and goat raw milk samples. The misuse of antibiotics in the livestock sector has already been reported to be a major factor contributing to AMR risk. For the study, milk samples were collected from five different farms, and metagenomic DNA was extracted. Then, PCR amplification was carried out using primers specific to 15 different AMR genes. From the results obtained, the prevalence of β-lactam resistance genes, particularly <i>blaTEM</i> (24%), along with other genes like <i>blaZ</i> (12%) and <i>blaSHV</i> (8%), were observed in addition to the transmissible <i>mcr9</i> gene (12%) conferring resistance to colistin. These findings underscore the urgent need for monitoring AMR genes and regulating antibiotic use in dairy farming to safeguard public health, as it poses a potential risk with the consumption of unpasteurized milk.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of Entomopathogenic Fungi for the Biocontrol of Tick Populations.","authors":"Ebrahim Abbasi","doi":"10.1089/fpd.2025.0057","DOIUrl":"https://doi.org/10.1089/fpd.2025.0057","url":null,"abstract":"<p><p><b><i>Background:</i></b> Ticks are significant vectors of various pathogens affecting humans and livestock, necessitating effective control strategies. The widespread use of chemical acaricides has led to resistance development and environmental concerns, highlighting the need for sustainable alternatives. Entomopathogenic fungi (EPF), particularly <i>Metarhizium anisopliae</i> and <i>Beauveria bassiana</i>, have emerged as promising biocontrol agents due to their pathogenicity against arthropods. This study evaluates the efficacy of these fungi in controlling tick populations under laboratory and field conditions. <b><i>Methods:</i></b> Fungal isolates of <i>M. anisopliae</i> and <i>B. bassiana</i> were cultured and applied to adult and nymphal ticks using direct immersion and topical application methods. Tick mortality was monitored over 14 days, and lethal time 50% (LT₅₀) and lethal concentration 50% (LC₅₀) values were determined using probit analysis. Environmental stability assays assessed conidial viability under different temperature and ultraviolet (UV) exposure conditions. A field trial was conducted to evaluate fungal efficacy in reducing tick populations in a natural setting. <b><i>Results:</i></b> Both <i>M. anisopliae</i> and <i>B. bassiana</i> induced significant mortality in ticks, with <i>M. anisopliae</i> exhibiting slightly higher virulence. LT₅₀ values were 5.8 ± 0.4 days for <i>M. anisopliae</i> and 6.9 ± 0.5 days for <i>B. bassiana</i>. Conidial viability declined under high temperatures and prolonged UV exposure, but fungal application in shaded areas improved efficacy. The field trial showed a 67.3% reduction in tick populations after fungal treatment (<i>p</i> < 0.001). <b><i>Discussion:</i></b> These findings demonstrate the potential of EPF as effective biocontrol agents for tick management. While environmental factors influence fungal persistence, protective formulations and targeted application strategies could enhance field performance. Integrating EPF with other control measures may provide a sustainable approach to tick population suppression.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the Dual Role of <i>Campylobacter jejuni:</i> From Pathogen to Potential Tumorigenesis Catalyst.","authors":"Amina Zergui, Mohamed Merzoug, Choubaila Leksir, Yamina Merzouk, Hanane Fatma Chentouf, Imane Djaballi","doi":"10.1089/fpd.2025.0003","DOIUrl":"https://doi.org/10.1089/fpd.2025.0003","url":null,"abstract":"<p><p><i>Campylobacter jejuni</i> is widely recognized as a leading cause of bacterial gastroenteritis worldwide. While its acute effects on human health are well documented, a growing body of research suggests that its impact extends far beyond transient infections. Emerging evidence indicates that <i>C. jejuni</i> may contribute to chronic inflammatory conditions and even tumorigenesis, particularly within the gastrointestinal tract. However, the potential role of this pathogen in cancer development remains underexplored, leaving a critical gap in the current literature. This review aims to bridge this gap by providing a comprehensive analysis of <i>C. jejuni</i>'s dual role as both a foodborne pathogen and a potential catalyst for long-term disease. We examine its transmission pathways, unique virulence factors, and evolving antimicrobial resistance patterns, highlighting their implications for public health. More importantly, we explore recent findings that suggest a mechanistic link between persistent <i>C. jejuni</i> infection, chronic inflammation, and carcinogenesis, focusing on its ability to induce deoxyribonucleic acid damage and immune evasion. By synthesizing insights from microbiology, epidemiology, and clinical research, this review offers a novel perspective on <i>C. jejuni</i>, shifting the focus beyond its acute pathogenicity to its broader health implications. In doing so, it underscores the urgent need for further studies to elucidate its oncogenic potential and inform new strategies for prevention and intervention. This review not only advances our understanding of <i>C. jejuni</i>'s impact on human health but also calls attention to a pressing yet overlooked research frontier in infectious disease and cancer biology.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144215397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Investigation of an Outbreak of <i>Salmonella</i> Newport Infections Linked to Melons from Southwest Indiana-United States, 2023.","authors":"Natalie Cataldo, Colin Schwensohn, Margaret Kirchner, Erin Jenkins, Temesgen Jemaneh, Sharon Seelman, Manashi Dey, Casey Hamblin, Darcy Brillhart, Alex Goodman, Kurt Nolte, Adam Baker, Tim Jackson, Bob Literman, Rebecca L Bell, Tracy Hawkins, Laura Gieraltowski, Monique Salter, Stelios Viazis","doi":"10.1089/fpd.2024.0171","DOIUrl":"https://doi.org/10.1089/fpd.2024.0171","url":null,"abstract":"<p><p>The Food and Drug Administration (FDA), the Centers for Disease Control and Prevention (CDC), and state health and regulatory partners have investigated outbreaks of <i>Salmonella enterica</i> serovar Newport and Typhimurium infections in 2012, 2020, 2022, and 2023 linked to cantaloupes and/or watermelons from Southwest Indiana. In 2023, an outbreak of <i>Salmonella</i> Newport infections included 11 ill people and five hospitalizations reported in six states. The FDA and CDC collaborated with state partners to investigate the outbreak. The traceback investigation did not identify a single point of convergence for cantaloupe or watermelon. Products and environmental samples collected by the FDA and state partners from farms, public land, distributors, and points of service yielded isolates of multiple <i>Salmonella</i> serovars, including <i>Salmonella</i> Typhimurium and <i>Salmonella</i> Newport, that matched by whole genome sequencing to the 2020, 2022, and 2023 outbreak strains. Due to limitations with the traceback investigation and lack of convergence, a specific type of melon or source of contamination was not identified. However, the laboratory findings and historical outbreak information provided additional evidence to support a finding of ongoing contamination issues for cantaloupes and watermelons grown in Southwest Indiana. This is the fourth outbreak of salmonellosis confirmed to be linked to cantaloupes and/or watermelons from this area since 2012. These outbreaks of reoccurring and persisting strains of <i>Salmonella</i> illustrate the urgent need to determine the source, pathway, and extent of environmental contamination in the melon-growing region of Southwest Indiana and for outreach and education to help promote practices to reduce the contamination of melons.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144198569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Antibacterial Efficacy and Mechanism of Tea Polyphenol Against Drug-Resistant <i>Aeromonas veronii</i> TH0426 <i>In Vitro</i>.","authors":"Liying Peng, Zongtao Chen, Yanting Hei, Wenyan Wei, Defang Chen","doi":"10.1089/fpd.2024.0072","DOIUrl":"10.1089/fpd.2024.0072","url":null,"abstract":"<p><p>The emergence of Motile Aeromonas Septicemia (MAS) caused by <i>Aeromonas veronii</i> in sturgeon farming has become a significant concern due to its high mortality impact on the aquaculture industry. The threat posed by MAS highlights the urgent need for effective control measures to combat bacterial infections in sturgeon populations. Tea polyphenol (TP) has demonstrated promising antibacterial properties against livestock and poultry bacterial infections. However, its antibacterial efficacy and mechanism in bacterial diseases of aquatic animals remain largely unexplored. This study aimed to investigate the <i>in vitro</i> antibacterial effect and mechanism of TP on fish-borne drug-resistant <i>A. veronii</i> TH0426 by assessing the impact of TP on TH0426 cell growth, antibiofilm activity, morphology, as well as measuring electrical conductivity, DNA extravasation, lactate dehydrogenase (LDH) activity, protein, and DNA contents. Results demonstrated that the minimum inhibitory concentration and the minimum bactericidal concentration of TP on TH0426 were 1024 and 2048 μg/mL, respectively. After a 4 h treatment, the growth of TH0426 was completely inhibited at the concentration of 1024 and 2048 μg/mL of TP. Meanwhile, TP exhibited a significant antibiofilm activity. Both scanning electron microscope and transmission electron microscope analyses revealed disrupted cell membrane structure, irregular cell morphology, and loss of intracellular contents following TP treatment. Moreover, increased cell membrane permeability induced by TP led to intracellular ion and DNA leakage, resulting in elevated electrical conductivity and DNA extravasation. Furthermore, TP decreased LDH activity, protein concentration and content, DNA fluorescence intensity, and density in a time-dependent manner, indicating inhibition of protein metabolism and DNA synthesis. In conclusion, TP exhibits potent antibacterial properties by inhibiting biofilm formation, disrupting cell membrane integrity, and interfering with protein metabolism and DNA synthesis in drug-resistant <i>A. veronii</i> TH0426 <i>in vitro</i>.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"422-429"},"PeriodicalIF":1.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142389178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Antibacterial Effect of Lactic Acid Bacteria and Baicalin Against <i>Staphylococcus aureus In Vitro</i> and <i>In Vivo</i>.","authors":"Yan-Ni Mao, Yan-Jun Ma, Gui-Qin Wang","doi":"10.1089/fpd.2024.0086","DOIUrl":"10.1089/fpd.2024.0086","url":null,"abstract":"<p><p>Pathogenic bacteria such as <i>Staphylococcus aureus</i> (<i>S. aureus</i>) are the principal cause of cow mastitis, which primarily impacts milk yield and results in significant financial losses for the animal husbandry industry. Lactic acid bacteria-cell-free supernatant (LAB-CFS) and baicalin (BAI) both have a number of biological effects, including decreasing inflammation. The combined use of LAB-CFS and BAI does not appear to have been used to protective against mastitis, however, and the underlying mechanisms are yet unknown. In this study, <i>in vitro</i> activity of LAB-CFS and BAI alone and in combination was determined (checkerboard experiments, time-kill curves, and flow cytometry to investigate membrane permeability) and examined the protective effects of LAB-CFS and BAI on <i>S. aureus</i>-induced mastitis in mice and the impact of NF-κB signaling pathways on the emergence of mastitis. We discovered that when LAB-CFS and BAI were used together, <i>S. aureus</i> was more effectively treated than when LAB-CFS and BAI were used separately. Flow cytometry demonstrated that LAB-CFS and BAI work together to kill bacteria. <i>In vivo</i>, the usage of LAB-CFS and BAI decreased the activity of myeloperoxidase, as well as IL-6, IL-1β, and TNF-α secretion and the levels of TLR2 and p65 (NF-κB) expression. These findings suggested that LAB-CFS and BAI had a preventive effect against mastitis brought on by <i>S. aureus</i>. Therefore, the NF-κB signaling pathway is thought to be the likely mechanism through which LAB-CFS and BAI reduced <i>S. aureus</i>-induced inflammation in the mammary of cows. For the treatment of cow mastitis, LAB-CFS and BAI are likely to replace antibiotics.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"376-384"},"PeriodicalIF":1.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142616986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infection Status, Etiological Analysis of <i>Aeromonas</i> spp. in Foodborne Diarrhea Patients from 2019 to 2023 in Wenzhou.","authors":"Huihuang Lou, Yi Li, Yuejin Wu, Yuqin Hu, Leyi Zhang","doi":"10.1089/fpd.2024.0030","DOIUrl":"10.1089/fpd.2024.0030","url":null,"abstract":"<p><p>The infection status and etiological analysis of <i>Aeromonas</i> spp. from foodborne diarrhea patients in Wenzhou were carried out to provide the etiological basis for healthy diet and clinical treatment. <i>Aeromonas</i> isolates (<i>n</i> = 41) collected from foodborne diarrhea patients were identified using the automatic bacteriologic analyzer and mass spectrometer. Species identification, multilocus sequence typing, prediction of virulence genes, and antimicrobial resistance genes were analyzed by the data of whole genome sequencing. The antibiotic resistance of these isolates was determined using miniaturization of the broth dilution susceptibility test. A total of 1829 stool samples of diarrhea patients were collected, and the detection rate of <i>Aeromonas</i> spp. was 2.24% (41/1829). Moreover, <i>Aeromonas</i> spp. are more easily detected in warmer months (from June to August), which were identified as follows: <i>A. veronii</i> (53.66%, 22/41), <i>A. caviae</i> (21.95%, 9/41), <i>A. hydrophila</i> (9.76%, 4/41), <i>A. dhakensis</i> (4.88%, 2/41), <i>A. rivipollensis</i> (4.88%, 2/41), <i>A. enteropelogenes</i> (2.44%, 1/41), and <i>A. media</i> (2.44%, 1/41). All strains can be divided into 38 sequence types, 31 of which were novel, suggesting that <i>Aeromonas</i> spp. had high genetic diversity, multiple clones, and various sources in diarrhea patients. High number of genetic diversity and resistance were found in the <i>Aeromonas</i> isolates. In addition, the category distribution of the virulence genes was significantly different among the seven species of <i>Aeromonas</i>. <i>Aeromonas</i> spp. had different degrees of resistance to antibiotics, and tetracycline was the most serious, with a resistance rate of 27%. What's more, for some antimicrobial classes <i>in silico</i> antimicrobial resistance gene detection was highly correlated with phenotypic antimicrobial resistance patterns with an overall sensitivity of 93.3% and a specificity of 66.7%. The findings from this research highlighted the importance for development of prevention and control strategies to reduce the risk of foodborne diarrhea caused by <i>Aeromonas</i> spp.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"414-421"},"PeriodicalIF":1.9,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}