Foodborne pathogens and disease最新文献

{"title":"Prevalence of Flesh-Eating Bacteria (<i>Vibrio Vulnificus</i>) Type A (Environmental) and Type B (Clinical) in Tilapia Fish Samples: Phenotypic and Molecular Characterizations.","authors":"Hany M Yehia, Nourhan H Mohamed, Shereen M Korany, Abeer S Aloufi, Manal F Elkadragy, Abdulhakeem Alzahrani, Mosffer M Al-Dagal","doi":"10.1089/fpd.2024.0147","DOIUrl":"https://doi.org/10.1089/fpd.2024.0147","url":null,"abstract":"<p><p><i>Vibrio vulnificus</i>, both types A and B, in tilapia fish causes several diseases in humans-septicemia, gastroenteritis, and wound infection-that primarily occur due to the consumption of raw or undercooked fish. Using fresh tilapia fish samples collected from Riyadh market in Saudi Arabia, this study attempted to phenotypically detect <i>V. vulnificus</i> using specific media, such as thiosulphate citrate bile salt sucrose agar, CHROMagar™ <i>Vibrio</i>, and cellobiose polymyxin colistin agar, while Api 20NE was employed Molecular detection was conducted using 16S rRNA gene sequencing, to characterize the clinical (genotype B) and environmental strains (genotype A) of <i>V. vulnificus</i>. Virulence genes, such as hemolysin A (<i>Vvh</i>A) and <i>Viu</i>B which encodes vulnibactin siderophores, were employed to detect both strains, in addition to capsular polysaccharide (<i>CPS</i>) and allele 1 genes. Virulence-correlated (<i>vcg</i>) genes <i>vcg</i>C and <i>vcg</i>E were also considered. The multidrug resistance of both strains to different antibiotics was tested, while whole-cell protein profiles were used to examine their differences based on Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis. The tilapia fish contained type A and B of <i>V. vulnificus</i> at a ratio of 12% and 4%, respectively. Generation times of the clinical and environmental strains were 14.45 min and 12.04 min, respectively. Analysis of the 16S rRNA, <i>Vvh</i>A, <i>CPS</i>, <i>Viu</i>B, <i>vcg</i>C, and <i>vcg</i>E in type A and B revealed that both strains comprised all virulent genes in different ratios. Raw tilapia fish contained <i>V. vulnificus</i> type A and B, so care in handling is essential or during cooking these kinds of fish.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence and Growth Potential of <i>Salmonella</i> in Pulps from Fresh Tomatoes of Various Cultivars.","authors":"Ana Carolina Bortolossi, Thaís G Magri, Dionisio P Amorim-Neto, Anderson S Sant'Ana","doi":"10.1089/fpd.2024.0123","DOIUrl":"https://doi.org/10.1089/fpd.2024.0123","url":null,"abstract":"<p><p>Brazil plays a significant role in the global tomato market and research into the microbiological characteristics and optimal storage conditions for this fruit is fundamental. Additionally, the recurrent outbreaks of salmonellosis linked to tomato consumption underscore the importance of understanding the prevalence and persistence of this bacterium in this product to ensure food safety. This study evaluated the incidence of <i>Salmonella</i> in commercially available tomatoes and determined the growth potential (δ) of a pool of <i>Salmonella</i> strains (<i>Salmonella</i> Enteritidis [503, 504], <i>Salmonella</i> Typhimurium [271], <i>Salmonella</i> Infantis [2883], and <i>Salmonella</i> Senftenberg [587]) on pulp of three tomato cultivars (Sweet Grape, Salad Sensation, and Débora) under different storage scenarios. None of the 240 tomato samples collected in Campinas (São Paulo State, Brazil) showed surface contamination by <i>Salmonella</i>. Tomato pulps of the Débora and Sweet Grape varieties, with lower pH values (3.0 and 4.0, respectively), inhibited the growth of <i>Salmonella</i> at all tested temperatures (10°C, 20°C, and 30°C). However, the Salad Sensation pulp (pH 6.0) allowed for <i>Salmonella</i> multiplication, especially at 30°C with δ of 2.88 ± 0.12 log10 colony-forming unit/g. These results indicate that pH is a critical factor for <i>Salmonella</i> growth in tomato pulps of different varieties stored at various temperatures.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143729652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of <i>Enterococcus faecium</i> Extracellular Vesicles on Intestinal Barrier Function and Microbiota in Piglets.","authors":"Meiying Luo, Junhang Sun, Suqian Li, Xin Feng, Huihua Zhang, Qien Qi","doi":"10.1089/fpd.2024.0133","DOIUrl":"https://doi.org/10.1089/fpd.2024.0133","url":null,"abstract":"<p><p><i>Enterococcus faecium</i> (Ef) is a common microorganism in the gastrointestinal tract, recognized for its beneficial effects on human and animal health. It plays a vital role in promoting intestinal microbial balance and enhancing gut barrier function. A key feature of Ef extracellular vesicles (EfEVs) is their ability to specifically target sites within the intestine, leading us to hypothesize that EfEVs function as an important active component of Ef in intestinal microenvironment regulation. This study aimed to explore the impact of EfEV supplementation on the intestinal barrier function and microbiota composition in piglets. <i>In vitro</i> intestinal porcine epithelial cell J2 (IPEC-J2) cell culture experiments have shown that supplementation with EfEV significantly enhanced cell viability, increased the activity of the antioxidant enzyme, promoted cell proliferation, and inhibited autophagy. <i>In vitro,</i> fecal culture experiments demonstrated significant increases in OD630 and the contents of acetic aid, butyric acid, and isovaleric acid, and decreases in valeric acid following EfEV supplementation. Furthermore, EfEV supplementation altered the diversity and composition of the intestinal microbiota. It significantly reduced levels of <i>Escherichia-Shigella</i> at the family level, while increasing the abundance of beneficial microorganisms, including <i>[Eubacterium]_eligens_group</i>, <i>unidentified_Mitochondria</i>, <i>Lachnoclostridium</i>. In conclusion, EfEV plays a pivotal role in inhibiting pathogens growth, enhancing the production of metabolites such as acetic acid and butyrate acid, providing energy for microorganisms, and protecting the intestinal barrier.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Cronobacter</i> Surveillance in Minnesota, United States, 2002-2024.","authors":"Joshua Rounds, Jennifer L Dale, Annastasia Gross, Laura E Tourdot, Paula Snippes Vagnone, Kirk Smith, Carlota Medus, Ruth Lynfield","doi":"10.1089/fpd.2024.0177","DOIUrl":"https://doi.org/10.1089/fpd.2024.0177","url":null,"abstract":"<p><p><i>Cronobacter sakazakii</i> is an opportunistic pathogen associated with bloodstream infections and meningitis in neonates and infants. <i>C. sakazakii</i> isolated from infants under 1 year of age has been reportable in Minnesota since 2003. Invasive <i>Cronobacter</i> infections in infants was recently made notifiable nationally in 2024. Therefore, this summary of over 20 years of reportable surveillance for <i>C. sakazakii</i> from Minnesota infants will inform other jurisdictions. During 2002-2024, 23 <i>C. sakazakii</i> cases in infants, including 8 invasive and 15 non-invasive cases, were identified and confirmed using MALDI-TOF or conventional biochemical methods. Invasive cases were younger (median, 18 days; range, 8 days to 10 months) than non-invasive cases (median, 98 days; range, 7 days to 8 months). <i>Cronobacter</i> isolates from infant (21), child (4), and adult (15) cases were submitted for whole genome sequencing (WGS) to obtain a contextual understanding of potential relatedness. In addition, <i>C. sakazakii</i> isolated from powdered infant formula (PIF) in 2003 was sequenced for comparison. Sequence type (ST) 4 was responsible for the majority of <i>C. sakazakii</i> cases overall (65%) and invasive infant cases (88%). Opened and unopened PIF was cultured from 10 investigations representing both invasive and non-invasive cases. Two (25%) opened PIF samples tested positive for <i>C. sakazakii</i> ST 4 while one (11%) unopened PIF sample tested positive for <i>C. sakazakii</i> ST 4. WGS demonstrated that no clinical cases were associated with a recognized outbreak. Several cases were likely due to contamination of PIF in the home during preparation, highlighting the importance of infant caregiver education to prevent infections.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Tracing and Molecular Characteristics of Strains from <i>Campylobacter jejuni</i> Bacteremia in a 23-Day-Old Infant in China.","authors":"Silu Peng, Wei Tong, Chengwei Liu, Houde Zhou, Jian Xiao, Daofeng Liu, Jun Lu","doi":"10.1089/fpd.2024.0161","DOIUrl":"https://doi.org/10.1089/fpd.2024.0161","url":null,"abstract":"<p><p><i>Campylobacter jejuni</i> (<i>C. jejuni</i>), a common foodborne zoonotic pathogen, usually causes gastroenteritis and rarely causes extraintestinal infections such as bacteremia. This study investigates a rare case of invasive <i>C. jejuni</i> bacteremia in a 23-day-old infant in Nanchang, China. Epidemiological data were collected from the infant's cohabitants (3). The cohabitants' anal swabs (3), milk powder (1), a feeding bottle (1), and kitchen items (3) were collected and screened for <i>C. jejuni</i>. Antimicrobial susceptibility testing was conducted, and whole genome sequencing was performed for genetic analysis, including whole genome multilocus sequence typing (wgMLST), single-nucleotide polymorphism, antimicrobial resistance (AMR) and virulence gene profiling. As a result, three <i>C. jejuni</i> strains were isolated from the infant, the infant's grandmother, and the feeding bottle. The results revealed that the three <i>C. jejuni</i> isolates were clonally related, sharing minimal genetic differences. The infant's grandmother, who had slaughtered a live chicken 4 days before the infant's illness, was identified as the likely source of transmission. AMR profiles showed resistance to fluoroquinolones and cephalosporins. The three isolates were found to carry the <i>blaOXA-184</i> gene and a chromosomal mutation in <i>gyrA</i> (T86I). Additionally, 69 virulence genes were identified in all isolates, including those associated with adherence (4), glycosylation system (23), motility and export apparatus (38), cytolethal toxin (3), and invasion (1). This case represents the third reported instance of <i>C. jejuni</i> bacteremia in an infant in China. The analysis confirmed a transmission pathway from the grandmother.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global Epidemiology and Subtype Distribution of <i>Blastocystis</i> sp. in Birds: A Systematic Review and Meta-Analysis.","authors":"Mohammad Reza Mohammadi, Ali Asghari, Milad Badri, Farajolah Maleki, Behzad Bijani, Amin Karampour","doi":"10.1089/fpd.2025.0007","DOIUrl":"https://doi.org/10.1089/fpd.2025.0007","url":null,"abstract":"<p><p>This systematic review and meta-analysis aimed to evaluate the global epidemiology, subtype (ST) distribution, and zoonotic potential of <i>Blastocystis</i> sp. in birds. A systematic review of the literature up to July 26, 2024, identified 47 articles/98 datasets meeting the inclusion criteria, covering data from 19 countries and over 41 bird species. Total estimates and 95% confidence intervals (CIs) were calculated using a random-effects model. The weighted prevalence of <i>Blastocystis</i> sp. in birds was 26.4% (95% CI: 22.5-30.6%), varying by location and species. The highest pooled prevalence was in guinea fowls at 83.3% (95% CI: 36.9-97.7%), followed by pheasants at 50.4%, flightless birds at 47.8%, peafowls at 41%, exotic birds at 34.4%, chickens at 24.9%, uncategorized birds at 24.4%, Anatidae birds at 24.3%, red-crowned cranes at 19.7%, quails at 15.8%, and pigeons at 12.8%. Subgroup analysis of <i>Blastocystis</i> sp. prevalence was performed based on publication years, countries, continents, WHO regions, and sample sizes. Birds can be the proper source of infection for 20 <i>Blastocystis</i> sp. STs (ST1-ST10, ST13, ST14, ST20, ST21, ST23-ST25, and ST27-ST29), with 12 STs (ST1-ST10, ST14, and ST23) capable of infecting humans. The findings revealed that birds should not be ignored in terms of the possible transmission of <i>Blastocystis</i> sp. zoonotic infections to humans. Moreover, parasitic infections, such as <i>Blastocystis</i> sp., can affect the health of poultry, causing gastrointestinal issues that impact growth and productivity, which requires special attention in this field.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic and Whole-Genome Sequencing-Based Profiling of Antimicrobial Resistance and Virulence in <i>Campylobacter</i> spp. Isolated from Human Stool in a Croatian University Hospital.","authors":"Luka Katic, Ivana Ferenčak, Ana Gverić-Grginić, Ivana Goic-Barisic, Zana Rubic, Marina Radic-Skelin, Jelena Marinovic, Dora Smolić, Josipa Kuzle, Lucija Škara Abramović, Irena Tabain, Dragan Jurić, Marija Tonkic, Georgina Osorio, Anita Novak","doi":"10.1089/fpd.2024.0173","DOIUrl":"https://doi.org/10.1089/fpd.2024.0173","url":null,"abstract":"<p><p><i>Campylobacter</i> spp. is a leading cause of community-acquired gastroenteritis, with rising antimicrobial resistance (AMR) posing a significant public health challenge. This study aimed to characterize the genomic basis of resistance and virulence in <i>Campylobacter</i> spp. isolated from human stool at the University Hospital of Split, Croatia. A total of 115 unduplicated isolates (100 <i>Campylobacter jejuni</i> and 15 <i>Campylobacter coli</i>) were identified and tested for antimicrobial susceptibility, from January to June 2023. A representative number of isolates with phenotypically detected resistance were analyzed with whole-genome sequencing. Multilocus sequence typing (MLST) was determined using genomic sequences as input data against the standard MLST schemes. High rates of resistance were detected to tetracycline (45% of <i>C. jejuni</i> and 26.7% of <i>C. coli</i>) and ciprofloxacin (81% of <i>C. jejuni</i> and 46.7% of <i>C. coli</i>), while combined ciprofloxacin/tetracycline resistance was observed in 43% of <i>C. jejuni</i> and 7% of <i>C. coli</i> isolates. The majority of whole-genome sequenced isolates possess <i>tet(O/32/O)</i> gene and <i>gyrA.T861</i> mutation, conferring resistance to tetracycline and fluoroquinolones. All isolates have at least one resistance gene for β-lactams (<i>bla</i>_OXA-61, <i>bla</i>_OXA-193, <i>bla</i>_OXA-450, <i>bla</i>_OXA-451, <i>bla</i>_OXA-452, <i>bla</i>_OXA-461, and <i>bla</i>_OXA-489), while 40% of isolates possess <i>ant(6)-Ia</i> for aminoglycoside resistance. In addition, a newly emerged, phenotypically identified multidrug-resistant <i>C. coli</i>, harbored <i>tet(O/32/O)</i> and <i>tet(O)</i> genes, point mutation in <i>gyrA</i> gene, and A2075G mutation in 23S rRNA. Virulence analysis highlighted <i>C. jejuni</i>'s broad pathogenic potential, including motility, adherence, invasion, exotoxin production, and immune modulation (e.g., <i>wlaN</i> gene, linked to Guillain-Barré syndrome). On the contrary, <i>C. coli</i> isolates possess genes exclusively for motility. Nine different <i>C. jejuni</i> MLST sequence types were identified, while all <i>C. coli</i> isolates belong to the same 828 clonal complex. Escalating AMR and a broad spectrum of virulence in <i>Campylobacter</i> spp. highlight the importance of continuous surveillance on the phenotypic and genotypic level, thereby allowing more efficient health care management of these infections.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Antiparasitic Effects of Nitrate and Lactic Acid on <i>Sarcocystis</i> Bradyzoites in Meat: Enhancing Food Safety.","authors":"Mortaza Rahimi, Nasser Hajipour, Parviz Hassanzadeh","doi":"10.1089/fpd.2024.0174","DOIUrl":"https://doi.org/10.1089/fpd.2024.0174","url":null,"abstract":"<p><p><i>Sarcocystis</i> spp. are zoonotic protozoan parasites found worldwide, transmitted through the consumption of infected meat. These parasites form cysts in the muscles of livestock, causing economic losses and health risks, including reduced meat and milk production, abortions, and, in severe cases, death in intermediate hosts. This study evaluates the antiparasitic effects of nitrate and lactic acid against <i>Sarcocystis</i> bradyzoites in sheep meat, aiming to identify effective decontamination methods to enhance food safety. Samples of <i>Sarcocystis</i> cysts were collected from infected sheep organs and treated with various concentrations (3%, 6%, 9%, and 12%) of nitrate and lactic acid for 3-48 h. Bradyzoite viability was assessed microscopically after staining and digestion. Additionally, bioassays were conducted using cats to confirm the treatment's efficacy. Results demonstrated that both compounds significantly increased bradyzoite mortality with higher concentrations and longer exposure times. Lactic acid showed greater efficacy at lower concentrations; 100% mortality was achieved at 3% lactic acid after 48 h and 6% after 24 h. In contrast, nitrate required 12% concentration for 100% mortality within 24 h. Statistical analysis revealed a significant correlation between compound concentration and exposure time with bradyzoite mortality, highlighting lactic acid's superior antiparasitic properties at lower concentrations compared with nitrate. This study highlights the potential of lactic acid and nitrate as natural decontamination agents for meat safety. Their application could mitigate <i>Sarcocystis</i>-related risks, reduce foodborne parasitic infections, and contribute to the economic sustainability of meat production.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Trends of <i>Vibrio cholerae:</i> Global and Regional Incidences.","authors":"Beenzu Siamalube, Emmanuel Ehinmitan, Steven Runo, Justus Onguso, Maina Ngotho","doi":"10.1089/fpd.2024.0165","DOIUrl":"https://doi.org/10.1089/fpd.2024.0165","url":null,"abstract":"","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Differentially Expressed Proteins in Bovine Mammary Glands Infected with <i>Staphylococcus aureus</i>.","authors":"Li Zhou, Zhuo-Ma Luoreng, Xing-Ping Wang","doi":"10.1089/fpd.2024.0179","DOIUrl":"https://doi.org/10.1089/fpd.2024.0179","url":null,"abstract":"<p><p>Mastitis is among the most prevalent diseases in dairy cows, leading to substantial issues such as decreased milk yield and quality, reduced reproductive performance, early culling, and increased production costs. <i>Staphylococcus aureus</i>, a naturally occurring opportunistic pathogen, is majorly responsible for mastitis in dairy cows. Mammary tissues from healthy cows were used as a control group (M_C, <i>n</i> = 3), and mammary glands of Chinese Holstein cows infected with <i>S. aureus</i> (10⁵ colony-forming unit/mL) were used as experimental groups (M_S, <i>n</i> = 3). Histopathological examination and analysis of inflammatory factor expression confirmed the successful establishment of the mastitis model. Using label-free quantitative proteomics, we analyzed the protein expression profiles of normal healthy mammary tissues (M_C) and <i>S. aureus</i>-infected mammary tissues (M_S). The analysis identified 933 differentially expressed proteins in <i>S. aureus</i>-infected mammary tissues, with 608 upregulated and 325 downregulated proteins, compared with the healthy tissues. Gene ontology functional annotation indicated that these proteins are involved in biological processes such as oxidation-reduction, the extracellular region, and catalytic activity. The Kyoto Encyclopedia of Genes and Genomes enrichment unveiled that these proteins were associated with pathways including <i>S. aureus</i> infection, cell adhesion molecules, antigen processing, and presentation. According to the InterPro enrichment analysis, the immunoglobulin-like fold and pleckstrin homology domain were the most enriched structural domains. Integrated transcriptomic and proteomic analyses revealed that ETNK1 and VNN1 exhibited consistent expression trends at both the mRNA and protein levels, suggesting their potential roles as key regulators in the pathogenesis of mastitis. The results not only enhance our understanding of mastitis management at the protein level but also point the way to potential antimastitis targets.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143556245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}