Foodborne pathogens and disease最新文献

筛选
英文 中文
Prevalence, Antimicrobial Resistance, and Genotypic Characterization of Diarrheagenic Escherichia coli in Foodborne Diarrhea Cases in Wenzhou Based on Whole-Genome Sequencing. 基于全基因组测序的温州市食源性腹泻病例致泻性大肠杆菌患病率、耐药性及基因型分析
IF 1.9 2区 农林科学
Foodborne pathogens and disease Pub Date : 2025-07-03 DOI: 10.1089/fpd.2025.0014
Panchi Shao, Xuejia Yang, Yuqin Hu, Huihuang Lou, Xuewei Liu, Haiyan Li, Leyi Zhang
{"title":"Prevalence, Antimicrobial Resistance, and Genotypic Characterization of Diarrheagenic <i>Escherichia coli</i> in Foodborne Diarrhea Cases in Wenzhou Based on Whole-Genome Sequencing.","authors":"Panchi Shao, Xuejia Yang, Yuqin Hu, Huihuang Lou, Xuewei Liu, Haiyan Li, Leyi Zhang","doi":"10.1089/fpd.2025.0014","DOIUrl":"https://doi.org/10.1089/fpd.2025.0014","url":null,"abstract":"<p><p><b><i>Background:</i></b> Although <i>Escherichia coli</i> can be an innocuous resident of the gastrointestinal tract, it also has the pathogenic capacity to cause significant diarrheal and extraintestinal diseases. The study aims to investigate the prevalence, antimicrobial resistance, and genotyping of diarrheagenic <i>Escherichia coli</i> (DEC) in foodborne diarrhea cases in Wenzhou City in 2023, providing a scientific foundation for the monitoring and prevention of bacterial foodborne diseases. <b><i>Method:</i></b> Fecal samples from patients with diarrhea were collected from four sentinel hospitals for the isolation of DEC. The pathogenic types of DEC were identified using fluorescence quantitative polymerase chain reaction. Antimicrobial susceptibility testing was performed by broth microdilution, while whole-genome sequencing was utilized for genotyping. Clustering analysis based on the EnteroBase database was conducted to assess the genetic similarity of the strains. <b><i>Results:</i></b> Among 2591 fecal samples tested, 90 were positive for DEC, resulting in a detection rate of 3.47% (90/2591). The majority of DEC isolates were enterotoxigenic <i>E. coli</i> (ETEC) and enteroaggregative <i>E. coli</i> (EAEC), accounting for 87.78% (79/90) of the total. DEC detection showed a seasonal pattern, with the highest positivity observed in individuals aged 20-39 years. The genotyping of the most prevalent virulence genes in ETEC was <i>estIa/estIb</i> (57.50%, 23/40), while <i>aggR</i> (23.08%) was most common in EAEC. Genotyping revealed high genetic polymorphism among DEC strains, with HC1100_13 being the predominant clone. The prevalence of the resistance gene <i>blaEC</i> was 96.67%. The highest drug resistance rate was observed in ampicillin (68.89%), followed by nalidixic acid (51.11%), and the multidrug resistance rate was 65.56%. <b><i>Conclusion:</i></b> ETEC and EAEC were the most common DEC strains detected in Wenzhou in 2023. These strains exhibited significant genetic diversity and displayed considerable antimicrobial and multidrug resistance, highlighting the need for enhanced monitoring and intervention strategies.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Epidemiology of Pentatrichomonas Hominis in Farmed Mink and Raccoon Dogs Across Five Provinces in China. 中国五省养殖水貂和貉中人五毛单胞菌的流行病学研究
IF 1.9 2区 农林科学
Foodborne pathogens and disease Pub Date : 2025-07-03 DOI: 10.1089/fpd.2025.0047
Na Lu, Hai-Tao Wang, Qing-Yu Hou, Ya Qin, Xue-Min Li, Xing Yang, Jing Jiang, Shuo Liu
{"title":"Epidemiology of <i>Pentatrichomonas Hominis</i> in Farmed Mink and Raccoon Dogs Across Five Provinces in China.","authors":"Na Lu, Hai-Tao Wang, Qing-Yu Hou, Ya Qin, Xue-Min Li, Xing Yang, Jing Jiang, Shuo Liu","doi":"10.1089/fpd.2025.0047","DOIUrl":"https://doi.org/10.1089/fpd.2025.0047","url":null,"abstract":"<p><p><i>Pentatrichomonas hominis</i> is a zoonotic pathogen with a wide host range, yet epidemiological data in mink and raccoon dogs remain sparse. This study investigates the prevalence of <i>P. hominis</i> in these species by analyzing 498 fecal samples collected from Hebei, Shandong, Liaoning, Jilin, and Heilongjiang provinces in China. Results revealed an overall infection rate of 9.64% (48/498, 95% confidence interval 7.19-12.58), with mink showing a 0.36% infection rate (1/276) and raccoon dogs 21.17% (47/222). Female animals had higher infection rates (9.87%, 47/476) than males (4.55%, 1/22). Adults exhibited higher infection rates (10.31%, 46/446) compared with juveniles (3.85%, 2/52). Phylogenetic analysis identified the CC1 genotype in all <i>P. hominis</i> detected. This study provides important epidemiological data on <i>P. hominis</i> infection in mink and raccoon dogs across five provinces, offering new insights into its regional distribution and transmission dynamics. These findings contribute to the development of more effective control strategies in the future.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144552810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rattus spp. as Reservoirs of Multidrug Resistance- and Biofilm-Forming Escherichia coli in Urban Community from Southern Thailand. 泰国南部城市社区多重耐药和形成生物膜的大肠杆菌储存库——家鼠
IF 1.9 2区 农林科学
Foodborne pathogens and disease Pub Date : 2025-07-01 Epub Date: 2024-12-04 DOI: 10.1089/fpd.2024.0109
Watcharapong Mitsuwan, Phirabhat Saengsawang, Sunsaneeya Thaikoed, Noppharat Tanthanathipchai, Pattarathai Saedan, Kittipong Chaisiri, Sumalee Boonmar, Yukio Morita
{"title":"<i>Rattus</i> spp. as Reservoirs of Multidrug Resistance- and Biofilm-Forming <i>Escherichia coli</i> in Urban Community from Southern Thailand.","authors":"Watcharapong Mitsuwan, Phirabhat Saengsawang, Sunsaneeya Thaikoed, Noppharat Tanthanathipchai, Pattarathai Saedan, Kittipong Chaisiri, Sumalee Boonmar, Yukio Morita","doi":"10.1089/fpd.2024.0109","DOIUrl":"10.1089/fpd.2024.0109","url":null,"abstract":"<p><p>Rats are rodents commonly found in Thailand that carry various zoonotic pathogens. Bacterial zoonosis can occur in a shared environment between humans and rats, especially in human communities and agricultural areas. <i>Escherichia coli</i>, particularly pathogenic and multidrug-resistant strains, is a significant public health concern that is transmitted by rats. This study aimed to investigate the antibiotic resistance (ABR) and biofilm formation of <i>E. coli</i> in caught rodents from Nakhon Si Thammarat province, Thailand. Captured rats were dissected to collect intestinal content for <i>E. coli</i> isolation. Two hundred and two confirmed <i>E. coli</i> were subjected for pathotype identification, antibiotic susceptibility testing, biofilm-forming ability (BFA), and the presence of related genes. Two <i>E. coli</i> isolates from intestinal content samples were atypical enteropathogenic (aEPEC). Predominantly, 52.97% of <i>E. coli</i> had azithromycin resistance, which was harbored by 35.64% of captured rats. Multidrug resistance (MDR) was found in 12.38% of <i>E. coli</i> isolates with 17 different MDR patterns. Remarkably, 96% of MDR isolates were resistant to azithromycin. Most <i>E. coli</i> harbored <i>ereA</i> (52%), followed by the <i>bla</i><sub>TEM</sub> and <i>aacC2</i> genes (6.44% each). Approximately 87% of isolated <i>E. coli</i> revealed moderate-to-high BFA. Predominantly, moderate-to-strong biofilm-forming <i>E. coli</i> harbored <i>pgaA</i> and <i>pgaC</i> genes. aEPEC, azithromycin resistance, MDR, and moderate-to-strong formation were the aspects of concern. Furthermore, the study of antibiotic-resistant <i>E. coli</i> in rats should be performed, particularly in terms of the transmission pathway, and the application of rats as bioindicators for ABR surveillance in Thailand should be established.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"514-523"},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142779737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Epidemiology of Ciguatoxin Poisoning Outbreaks in the United States Between 2001 and 2021. 2001 年至 2021 年美国爆发的雪茄毒素中毒流行病学。
IF 1.9 2区 农林科学
Foodborne pathogens and disease Pub Date : 2025-07-01 Epub Date: 2024-10-10 DOI: 10.1089/fpd.2024.0115
Andrew Huff, Colin Plover, Harriet Okatch, Brandon George
{"title":"Epidemiology of Ciguatoxin Poisoning Outbreaks in the United States Between 2001 and 2021.","authors":"Andrew Huff, Colin Plover, Harriet Okatch, Brandon George","doi":"10.1089/fpd.2024.0115","DOIUrl":"10.1089/fpd.2024.0115","url":null,"abstract":"<p><p>This study examined data from the Centers for Disease Control and Prevention's National Outbreak Reporting System between 2001 and 2021 for confirmed outbreaks of foodborne illness due to Ciguatoxin in the United States. Previous research into Ciguatoxin illness in the United States has focused on specific states or territories-namely Hawaii, Florida, Puerto Rico, and the Virgin Islands-meaning the national distribution of outbreaks remains unclear. In addition, while specific categories of seafood (e.g., reef fish) are known to carry elevated risk of illness due to Ciguatoxin poisoning, it remains unclear the extent to which specific seafood items are associated with the odds of being hospitalized in an outbreak. This study calculated descriptive statistics for the distribution of outbreaks by state, season, site of exposure, and implicated seafood item. Then, binomial regression was used to assess the relationship between the implicated seafood item in an outbreak and hospitalization. Results provided evidence that knowing the implicated seafood item in an outbreak is associated with the odds of hospitalization (χ<sup>2</sup><sub>5</sub> = 33.023, <i>p</i> < 0.0001). Even when a seafood item was found to be associated with elevated odds of hospitalization, not all cases involved hospitalization. This finding aligns with Ciguatoxin poisoning case reports, noting that key epidemiological factors include not just the seafood item consumed but also the quantity and the part consumed (e.g., the head). In conclusion, public health officials should consider Ciguatoxin poisoning outbreaks as multidimensional and utilize information about the seafood consumed as well as the quantity and parts of the seafood consumed.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"445-451"},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Clinical Manifestations and Molecular Identification of Giardia duodenalis in Pediatric and Adolescent Cancer Patients in Southwestern Iran. 伊朗西南部儿童和青少年癌症患者的临床表现和十二指肠贾第虫的分子鉴定。
IF 1.9 2区 农林科学
Foodborne pathogens and disease Pub Date : 2025-07-01 Epub Date: 2024-10-15 DOI: 10.1089/fpd.2024.0100
Farzad Mahdavi, Mohammad Reza Mohammadi, Kambiz Karimi, Laya Shamsi, Farajolah Maleki, Ali Asghari, Saeed Shahabi, Mohammad Hossein Motazedian, Hassan Nourmohammadi
{"title":"Clinical Manifestations and Molecular Identification of <i>Giardia duodenalis</i> in Pediatric and Adolescent Cancer Patients in Southwestern Iran.","authors":"Farzad Mahdavi, Mohammad Reza Mohammadi, Kambiz Karimi, Laya Shamsi, Farajolah Maleki, Ali Asghari, Saeed Shahabi, Mohammad Hossein Motazedian, Hassan Nourmohammadi","doi":"10.1089/fpd.2024.0100","DOIUrl":"10.1089/fpd.2024.0100","url":null,"abstract":"<p><p>This study aimed to investigate the clinical and molecular characteristics of <i>Giardia duodenalis</i> (<i>G. duodenalis</i>) infection and identify potential risk factors in children and teenagers with malignancies in Shiraz, southwestern Iran. A total of 200 fresh fecal samples were collected from children and adolescents suffering from 32 different cancer types at Amir, Nemazee, and Saadi hospitals affiliated with Shiraz University of Medical Sciences between October 2021 and May 2022. Direct microscopy using saline and iodine wet mount was conducted, and all fecal samples were rechecked by <i>SSU</i>-PCR. Subsequently, a specific fragment of the <i>tpi</i> gene was amplified on all samples for prevalence, sequencing, and assemblage identification. Our study found a 4% (8/200) prevalence of <i>G. duodenalis</i> using microscopy and PCR. The molecular findings were consistent with the microscopic results. All eight positive samples with <i>SSU-rRNA</i> gene were also detected as positive with <i>tpi</i> gene and were correctly sequenced. Among the examined cancer patients, two assemblages were identified: A [sub-assemblage AI (2/8, 25%) and sub-assemblage AII (3/8, 37.5%)] and B [sub-assemblage BIV (3/8, 37.5%)]. Notably, patients were more vulnerable to <i>G. duodenalis</i> infection after receiving at least 8 treatment episodes (<i>p</i> < 0.05) and displaying gastrointestinal symptoms (<i>p</i> > 0.05). The demographic characteristics of cancer patients with <i>G. duodenalis</i> infection and the statistical conclusions were separately detailed. The small sample size and low prevalence rate in this study hindered precise epidemiological conclusions. Nonetheless, the results suggest that <i>G. duodenalis</i> infection among cancer patients in Shiraz city originates from humans, without any specific animal groups (C-H) involved.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"498-504"},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142461625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transcriptomic Analysis of the Effect of Glabridin on Biofilm Formation in Staphylococcus Aureus. 格拉布林对金黄色葡萄球菌生物膜形成影响的转录组分析
IF 1.9 2区 农林科学
Foodborne pathogens and disease Pub Date : 2025-07-01 Epub Date: 2024-10-17 DOI: 10.1089/fpd.2024.0038
Yanjun Ma, Yanni Mao, Xinyun Kang, Beibei Zhang, Jianchong Wang, Guiqin Wang, Guilai Wang
{"title":"Transcriptomic Analysis of the Effect of Glabridin on Biofilm Formation in <i>Staphylococcus Aureus</i>.","authors":"Yanjun Ma, Yanni Mao, Xinyun Kang, Beibei Zhang, Jianchong Wang, Guiqin Wang, Guilai Wang","doi":"10.1089/fpd.2024.0038","DOIUrl":"10.1089/fpd.2024.0038","url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> (<i>S. aureus</i>) is among the major skin infection-causing pathogens in animals and humans. Its ability to form biofilms has become a foremost cause of bacterial infections and the extensive spread of drug resistance, which poses a great difficulty in clinical treatment. Glabridin (Glb), an extract of licorice with antibacterial and anti-infective properties, has a partially understood biofilm-inhibitory mechanism. This study investigated the inhibitory and antibiofilm activities of subinhibitory concentrations of Glb against <i>S. aureus</i>. The crystal violet assay revealed that Glb significantly suppressed biofilm expression. Scanning electron microscopy observations unveiled that Glb reduced <i>S. aureus</i> adhesion and accumulation by disrupting the spatial structure of the biofilm. In vitro extracellular DNA (eDNA) inhibition assays demonstrated that Glb inhibited biofilm formation by <i>S. aureus by</i> suppressing eDNA secretion. In total, 184 differentially expressed genes were obtained through transcriptomic (RNA-seq) sequencing, of which 81 and 103 genes were upregulated and downregulated, respectively. Glb regulated the transcript levels of biofilm-related genes through the phosphatase transfer system, two-component regulatory system, and nitrogen metabolism. The qPCR analysis was performed to confirm whether Glb interfered with the expression of regulatory genes involved in <i>S. aureus</i> biofilm formation (<i>SarA, ArlR, FnbA, ClfA, icaD,</i> and <i>icaR</i>) as well as the virulence gene <i>Hla</i>. In conclusion, this study demonstrates that Glb has a significant inhibitory effect on biofilm activity and is expected to be a good antibiofilm drug.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"489-497"},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142461631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Do Temperature Abuses Along the Frozen Açaí Pulp Value Chain Increase Microbial Hazards? 冷冻阿萨伊果浆价值链中的温度滥用是否会增加微生物危害?
IF 1.9 2区 农林科学
Foodborne pathogens and disease Pub Date : 2025-07-01 Epub Date: 2024-10-11 DOI: 10.1089/fpd.2024.0073
Jéssica Caroline Araujo Silva Sandes, Eduardo Henrique Miranda Walter, Gustavo Luis de Paiva Anciens Ramos, Virgínia Martins da Matta, Lourdes Maria Correa Cabral
{"title":"Do Temperature Abuses Along the Frozen Açaí Pulp Value Chain Increase Microbial Hazards?","authors":"Jéssica Caroline Araujo Silva Sandes, Eduardo Henrique Miranda Walter, Gustavo Luis de Paiva Anciens Ramos, Virgínia Martins da Matta, Lourdes Maria Correa Cabral","doi":"10.1089/fpd.2024.0073","DOIUrl":"10.1089/fpd.2024.0073","url":null,"abstract":"<p><p>Does temperature abuse during storage, distribution, marketing, and consumption of unpasteurized frozen açaí pulp increase microbial hazards? This study investigated the behavior of potentially pathogenic (<i>Escherichia coli, Listeria monocytogenes and Salmonella</i> spp.) and spoilage (mesophilic bacteria, yeasts and molds) microorganisms in two simulated thawing conditions: under refrigeration and at room temperature. The effect of repeated cold chain abuse was observed by thawing and refreezing (-20°C) açaí pulp four times over a period of 90 days. Freezing resulted in inhibition of all microorganisms except for mesophilic aerobic bacteria in one single sample. After thawing at 5°C, the kinetic parameters obtained by the Weibull model indicated that mesophilic aerobic bacteria, yeasts and molds and <i>L. monocytogenes</i> showed a longer inactivation time with <i>δ</i> values reaching 35, 126, and 46 days, respectively. The shortest inactivation time for a reduction of 4 log CFU.g<sup>-1</sup> was for <i>E. coli</i>. The concentration of <i>Salmonella</i> spp. and <i>L. monocytogenes</i> in control samples was higher (<i>p</i> < 0.01) than in samples exposed to abusive conditions after 90 days of storage. The results indicate that the abusive thawing conditions studied do not increase the potential hazards of pathogens.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"452-458"},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142406310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Prevalence, Risk Factors, and Antimicrobial Resistance of Staphylococcus and Streptococcus Species Isolated from Subclinical Bovine Mastitis. 从亚临床牛乳腺炎中分离出的葡萄球菌和链球菌的流行率、风险因素和抗菌药耐药性。
IF 1.9 2区 农林科学
Foodborne pathogens and disease Pub Date : 2025-07-01 Epub Date: 2024-10-31 DOI: 10.1089/fpd.2024.0097
Abu Al Farabi, Hemayet Hossain, Khadiza A Brishty, Md Hafizar Rahman, Mostafizor Rahman, Md Saiful Islam Siddiqui, Mohammad Atikuzzaman, Asmaa Saleh, Reem Binsuwaidan, Heba Mohammed Refat M Selim, Ayman Noreddin, Yosra A Helmy, Md Mahfujur Rahman, Himel Barua, Mohamed E El Zowalaty
{"title":"Prevalence, Risk Factors, and Antimicrobial Resistance of <i>Staphylococcus</i> and <i>Streptococcus</i> Species Isolated from Subclinical Bovine Mastitis.","authors":"Abu Al Farabi, Hemayet Hossain, Khadiza A Brishty, Md Hafizar Rahman, Mostafizor Rahman, Md Saiful Islam Siddiqui, Mohammad Atikuzzaman, Asmaa Saleh, Reem Binsuwaidan, Heba Mohammed Refat M Selim, Ayman Noreddin, Yosra A Helmy, Md Mahfujur Rahman, Himel Barua, Mohamed E El Zowalaty","doi":"10.1089/fpd.2024.0097","DOIUrl":"10.1089/fpd.2024.0097","url":null,"abstract":"<p><p>Subclinical mastitis (SCM) is a prevalent serious disease among dairy cows worldwide. It poses a significant challenge to the dairy industry, animal welfare, and a threat to public health. The present study aimed to investigate the molecular detection, prevalence, and antimicrobial resistance of <i>Staphylococcus</i> spp. and <i>Streptococcus</i> spp. isolated from raw composite milk samples obtained from SCM dairy cattle in Bangladesh. A total of 612 quarters milk samples obtained from 153 cows were analyzed for SCM using the California Mastitis Test. Bacterial isolation and identification were carried out and bacterial species were confirmed using molecular polymerase chain reaction methods. Antimicrobial susceptibility testing was performed using disc diffusion method. The findings revealed that the prevalence of SCM was 70.3% (26/37), 35.95% (55/153), and 23.04% (141/612) in the herd, cow, and quarter levels, respectively. Among the positive samples, 92.7% (51/55) were <i>Staphylococcus</i> spp. (<i>S. aureus, S. chromogenes</i>, and <i>S. simulans</i>) and the remaining isolates were 7.3% (4/55) <i>Streptococcus</i> spp. (<i>Streptococcus agalactiae</i> and <i>Streptococcus dysgalactiae</i>). The most prevalent species was <i>S. chromogenes,</i> accounting for 67.3% (37/55). Antimicrobial susceptibility testing showed that 65.5% of isolates were susceptible to cefoxitin, whereas, 89.1% were resistant to penicillin. Overall, 12 isolates (21.8%) out of 55 were resistant to more than three classes of antimicrobials and were defined as multidrug-resistant isolates. Methicillin-resistance gene was detected in 61.1% of the cefoxitin-resistant isolates. A multivariate logistic regression analysis identified five potential risk factors including the lack of post-milking teat disinfection (OR: 3.06), absence of immediate feeding after milking (OR: 9.81), poor udder hygiene (OR: 7.83), tick infestation (OR: 13.76), and absence of dry cow therapy (OR: 3.31). The findings of the current study underscore the urgent requirement for targeted interventions, considering the identified factors to effectively manage and control SCM in dairy cows.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"467-476"},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142544658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Experience and Perspectives on Antibacterial Therapy for Listeriosis in a Tertiary Teaching Hospital in China: A Retrospective Study and Health Care Provider Survey. 中国某三级教学医院李斯特菌病抗菌治疗的经验与展望:回顾性研究与医护人员调查。
IF 1.9 2区 农林科学
Foodborne pathogens and disease Pub Date : 2025-07-01 Epub Date: 2025-01-08 DOI: 10.1089/fpd.2024.0079
Naiju Zhang, Zhenjie Wang, Zhijun Li, Yongzhong Xie, Jinchun Liu, Chunming Gao, Chuanmiao Liu, Tianping Chen
{"title":"Experience and Perspectives on Antibacterial Therapy for Listeriosis in a Tertiary Teaching Hospital in China: A Retrospective Study and Health Care Provider Survey.","authors":"Naiju Zhang, Zhenjie Wang, Zhijun Li, Yongzhong Xie, Jinchun Liu, Chunming Gao, Chuanmiao Liu, Tianping Chen","doi":"10.1089/fpd.2024.0079","DOIUrl":"10.1089/fpd.2024.0079","url":null,"abstract":"<p><p>Listeriosis is a rare, life-threatening bacterial infection. Prompt, appropriate antibiotic treatment is essential to save lives. We aimed to investigate antibiotic selection and listeriosis knowledge among health care providers. We first performed a retrospective study on patients with listeriosis who were treated in a hospital in China from January 2013 to March 2023. Patient characteristics and antibiotic selections were recorded. Antibiotics were classified as effective or ineffective based on the efficacy classifications listed in <i>The Sanford Guide to Antimicrobial Therapy</i> (50<sup>th</sup> edition). We then conducted a questionnaire survey of health care providers to investigate their listeriosis knowledge and practices between April 2023 to May 2023. Fifteen listeriosis patients were identified, with a case fatality rate of 26.7% (4/15). Empirical treatments assessed as effective antibiotics or ineffective antibiotics were given to 33.3% (5/15) and 66.7% (10/15) of the patients, respectively. After <i>Listeria monocytogenes</i> was identified, 40% (6/15) of the patients received an adjustment to their antibiotics, although 50% (3/6) patients still received ineffective antibiotics. The questionnaire survey of 77 participating health care providers showed that 32.9% (24/73) of the clinicians were unaware of the appropriate antibiotics for listeriosis. Most participants (83.1%, 64/77) would read the drug sensitivity report before selecting the antibiotics. The selection of effective antibiotics differed between the infectious diseases department and other departments (<i>p</i> < 0.001), but did not differ between junior title and other titles (<i>p</i> = 0.088). Most patients with listeriosis did not receive appropriate antibiotics, which might have accounted for the high fatality rate, and may have resulted from inadequate listeriosis knowledge. More education and correct reporting of drug sensitivity results are urgently needed.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"505-513"},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142947051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
CRISPR/Cas12 System-Based Assay for Rapid, Sensitive Detection of Rotavirus in Food Samples. 基于 CRISPR/Cas12 系统的检测方法,用于快速、灵敏地检测食品样本中的轮状病毒。
IF 1.9 2区 农林科学
Foodborne pathogens and disease Pub Date : 2025-07-01 Epub Date: 2024-10-14 DOI: 10.1089/fpd.2024.0078
Shirui Gou, Yan Liu, Qianqian Li, Jielin Yang, Long Qiu, Yu Zhao
{"title":"CRISPR/Cas12 System-Based Assay for Rapid, Sensitive Detection of Rotavirus in Food Samples.","authors":"Shirui Gou, Yan Liu, Qianqian Li, Jielin Yang, Long Qiu, Yu Zhao","doi":"10.1089/fpd.2024.0078","DOIUrl":"10.1089/fpd.2024.0078","url":null,"abstract":"<p><p>Foodborne viruses have become an important threat to food safety and human health. Among the foodborne viruses, group A rotavirus is the most important pathogen of diarrhea in autumn and winter. The field detection of rotavirus is crucial for the early control of infection and patient management. Quantitative real-time reverse transcription-polymerase chain reaction is the most widely used in virus detection. However, the technique relies on high-cost instruments and trained personnel, which limit its use in field detection. In this study, we developed accurate, realizable, and simple detection methods by combining optimized CRISPR (clustered regularly interspaced short palindromic repeats) Cas12 and reverse transcription loop-mediated isothermal amplification (RT-LAMP) (reverse transcription loop-mediated isothermal amplification) to reduce the requirements for temperature control and costly real-time fluorescence polymerase chain reaction instruments. We investigated two nucleic acid detection systems combining RT-LAMP with CRISPR Cas12a and RT-LAMP with CRISPR Cas12b and compared them with reverse transcription-quantitative polymerase chain reaction. The resulting detection system only needs a reaction temperature and in single tube to react for 60 min with the detection sensitivity of 38 copies/μL. Overall, this study developed an innovative method for the rapid detection of rotavirus in food samples, which will help to effectively identify food contaminated by pathogens and prevent human infections and economic losses caused by disease outbreaks.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"459-466"},"PeriodicalIF":1.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142461627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信