Clinical Manifestations and Molecular Identification of Giardia duodenalis in Pediatric and Adolescent Cancer Patients in Southwestern Iran.

IF 1.9 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY
Farzad Mahdavi, Mohammad Reza Mohammadi, Kambiz Karimi, Laya Shamsi, Farajolah Maleki, Ali Asghari, Saeed Shahabi, Mohammad Hossein Motazedian, Hassan Nourmohammadi
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Abstract

This study aimed to investigate the clinical and molecular characteristics of Giardia duodenalis (G. duodenalis) infection and identify potential risk factors in children and teenagers with malignancies in Shiraz, southwestern Iran. A total of 200 fresh fecal samples were collected from children and adolescents suffering from 32 different cancer types at Amir, Nemazee, and Saadi hospitals affiliated with Shiraz University of Medical Sciences between October 2021 and May 2022. Direct microscopy using saline and iodine wet mount was conducted, and all fecal samples were rechecked by SSU-PCR. Subsequently, a specific fragment of the tpi gene was amplified on all samples for prevalence, sequencing, and assemblage identification. Our study found a 4% (8/200) prevalence of G. duodenalis using microscopy and PCR. The molecular findings were consistent with the microscopic results. All eight positive samples with SSU-rRNA gene were also detected as positive with tpi gene and were correctly sequenced. Among the examined cancer patients, two assemblages were identified: A [sub-assemblage AI (2/8, 25%) and sub-assemblage AII (3/8, 37.5%)] and B [sub-assemblage BIV (3/8, 37.5%)]. Notably, patients were more vulnerable to G. duodenalis infection after receiving at least 8 treatment episodes (p < 0.05) and displaying gastrointestinal symptoms (p > 0.05). The demographic characteristics of cancer patients with G. duodenalis infection and the statistical conclusions were separately detailed. The small sample size and low prevalence rate in this study hindered precise epidemiological conclusions. Nonetheless, the results suggest that G. duodenalis infection among cancer patients in Shiraz city originates from humans, without any specific animal groups (C-H) involved.

伊朗西南部儿童和青少年癌症患者的临床表现和十二指肠贾第虫的分子鉴定。
本研究旨在调查伊朗西南部设拉子地区患有恶性肿瘤的儿童和青少年感染贾第虫(G. duodenalis)的临床和分子特征,并确定潜在的风险因素。2021 年 10 月至 2022 年 5 月期间,设拉子医科大学附属阿米尔医院、内马兹医院和萨迪医院共收集了 200 份新鲜粪便样本,这些样本来自患有 32 种不同癌症的儿童和青少年。使用生理盐水和碘湿装片进行直接显微镜检查,并通过 SSU-PCR 对所有粪便样本进行复查。随后,对所有样本的 tpi 基因的特定片段进行扩增,以确定流行率、测序和组合。通过显微镜检查和 PCR 检测,我们的研究发现杜氏痢疾杆菌的流行率为 4%(8/200)。分子检测结果与显微镜检测结果一致。所有 8 个 SSU-rRNA 基因阳性样本的 tpi 基因也被检测为阳性,并被正确测序。在接受检查的癌症患者中,发现了两个集合体:A组[AI亚组(2/8,25%)和AII亚组(3/8,37.5%)]和B组[BIV亚组(3/8,37.5%)]。值得注意的是,接受至少 8 次治疗(P < 0.05)和出现胃肠道症状(P > 0.05)的患者更容易感染十二指肠球菌。另外还详细介绍了感染十二指肠球菌的癌症患者的人口统计学特征和统计结论。这项研究的样本量较小,患病率较低,因此无法得出准确的流行病学结论。不过,研究结果表明,设拉子市癌症患者中的十二指肠球菌感染源于人类,不涉及任何特定的动物群体(C-H)。
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来源期刊
Foodborne pathogens and disease
Foodborne pathogens and disease 医学-食品科技
CiteScore
5.30
自引率
3.60%
发文量
80
审稿时长
1 months
期刊介绍: Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.
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