Transcriptomic Analysis of the Effect of Glabridin on Biofilm Formation in Staphylococcus Aureus.

IF 1.9 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY
Yanjun Ma, Yanni Mao, Xinyun Kang, Beibei Zhang, Jianchong Wang, Guiqin Wang, Guilai Wang
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Abstract

Staphylococcus aureus (S. aureus) is among the major skin infection-causing pathogens in animals and humans. Its ability to form biofilms has become a foremost cause of bacterial infections and the extensive spread of drug resistance, which poses a great difficulty in clinical treatment. Glabridin (Glb), an extract of licorice with antibacterial and anti-infective properties, has a partially understood biofilm-inhibitory mechanism. This study investigated the inhibitory and antibiofilm activities of subinhibitory concentrations of Glb against S. aureus. The crystal violet assay revealed that Glb significantly suppressed biofilm expression. Scanning electron microscopy observations unveiled that Glb reduced S. aureus adhesion and accumulation by disrupting the spatial structure of the biofilm. In vitro extracellular DNA (eDNA) inhibition assays demonstrated that Glb inhibited biofilm formation by S. aureus by suppressing eDNA secretion. In total, 184 differentially expressed genes were obtained through transcriptomic (RNA-seq) sequencing, of which 81 and 103 genes were upregulated and downregulated, respectively. Glb regulated the transcript levels of biofilm-related genes through the phosphatase transfer system, two-component regulatory system, and nitrogen metabolism. The qPCR analysis was performed to confirm whether Glb interfered with the expression of regulatory genes involved in S. aureus biofilm formation (SarA, ArlR, FnbA, ClfA, icaD, and icaR) as well as the virulence gene Hla. In conclusion, this study demonstrates that Glb has a significant inhibitory effect on biofilm activity and is expected to be a good antibiofilm drug.

格拉布林对金黄色葡萄球菌生物膜形成影响的转录组分析
金黄色葡萄球菌(S. aureus)是导致动物和人类皮肤感染的主要病原体之一。其形成生物膜的能力已成为细菌感染和耐药性广泛传播的主要原因,给临床治疗带来了极大的困难。Glabridin (Glb)是一种具有抗菌和抗感染特性的甘草提取物,其抑制生物膜的机制已被部分了解。本研究调查了亚抑制浓度的 Glb 对金黄色葡萄球菌的抑制和抗生物膜活性。水晶紫试验显示,Glb 能显著抑制生物膜的表达。扫描电子显微镜观察显示,Glb 通过破坏生物膜的空间结构减少了金黄色葡萄球菌的粘附和积聚。体外细胞外DNA(eDNA)抑制实验表明,Glb通过抑制eDNA分泌来抑制金黄色葡萄球菌形成生物膜。通过转录组(RNA-seq)测序共获得了184个差异表达基因,其中上调基因81个,下调基因103个。Glb通过磷酸酶转移系统、双组分调控系统和氮代谢调控生物膜相关基因的转录水平。qPCR 分析证实了 Glb 是否干扰了参与金黄色葡萄球菌生物膜形成的调控基因(SarA、ArlR、FnbA、ClfA、icaD 和 icaR)以及毒力基因 Hla 的表达。总之,本研究表明,Glb 对生物膜活性有显著的抑制作用,有望成为一种良好的抗生物膜药物。
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来源期刊
Foodborne pathogens and disease
Foodborne pathogens and disease 医学-食品科技
CiteScore
5.30
自引率
3.60%
发文量
80
审稿时长
1 months
期刊介绍: Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.
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