Foodborne pathogens and disease最新文献

{"title":"Occurrence and Characteristics of Toxigenic <i>Staphylococcus aureus</i> in Retail Foods in Iran.","authors":"Erfan Ghoreyshizadeh, Meysam Manouchehrifar, Rashid Ramazanzadeh, Hadi Peeri Doghaheh, Mojtaba Amani, Mohsen Arzanlou","doi":"10.1089/fpd.2023.0122","DOIUrl":"10.1089/fpd.2023.0122","url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> causes various toxigenic and invasive diseases in humans worldwide. This study examined the prevalence, virulence genes, and antibiotic resistance of <i>S. aureus</i> isolates collected from 894 retail food samples in Ardabil, Iran. Staphylococcal cassette chromosome <i>mec</i> (SCC<i>mec</i>), <i>spa</i>, and multilocus sequence typing methods were employed to further investigate the molecular characteristics of methicillin-resistant <i>S. aureus</i> (MRSA) isolates. The results revealed that 11.18% (<i>n</i> = 100) of food samples exhibited contamination with <i>S. aureus</i> (10.50% methicillin-sensitive <i>S. aureus</i> [MSSA] and 0.67% MRSA). Notably, raw minced meat (29.41%), Faloodeh (25%), and Olivier salad (21.42%) emerged as the most frequently contaminated food items. Among the 100 isolates of <i>S. aureus</i>, 94% were characterized as MSSA, with the remaining 6% identified as MRSA. The highest resistance was observed for penicillin (12%). MRSA isolates exhibited significantly higher resistance rates. Seventy-nine percent of the isolates were positive for <i>sea</i>, 14% for <i>seb</i>, 8% for a <i>sec</i>, and 0% for <i>sed</i> enterotoxin-encoding genes. Sixteen percent of isolates harbored two or more staphylococcal enterotoxin genes, simultaneously. Moreover, 97%, 94%, 24%, and 22% of isolates were positive for <i>hla</i>, <i>hld</i>, <i>tst</i>, and <i>pvl</i> virulence-encoding genes, respectively. No isolate was positive for the exfoliative toxins encoding <i>eta</i> and <i>etb</i> genes. MRSA isolates belonged to CC8 (<i>n</i> = 4) and CC22 (<i>n</i> = 2). Isolates in CC8 belonged to lineage ST239-MRSA-III and <i>spa</i> type t030; the isolates in CC22 belonged to ST22-MRSA-IV and <i>spa</i> types t310 and t223. In conclusion, a relatively high proportion of our retail food samples were contaminated with <i>S. aureus</i>. The high incidence of isolates with toxigenic genes raises serious health concerns. Furthermore, the presence of MRSA lineages linked to humans suggests that retail foods may be contaminated with human origin.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"331-338"},"PeriodicalIF":2.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139722261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological and Whole Genome Sequencing Analysis of Restaurant <i>Salmonella</i> Enteritidis Outbreak Associated with an Infected Food Handler in Jiangxi Province, China, 2023.","authors":"Silu Peng, Huomei Xiong, Jun Lu, Fei Luo, Chengwei Liu, Houde Zhou, Wei Tong, Zhilu Xia, Daofeng Liu","doi":"10.1089/fpd.2023.0123","DOIUrl":"10.1089/fpd.2023.0123","url":null,"abstract":"<p><p>In China, <i>Salmonella</i> is one of the most frequent causes of bacterial gastroenteritis, and food handlers in restaurants as an important contaminated source were rarely reported. In May 2023, an outbreak of <i>Salmonella enterica</i> serovar Enteritidis infection in a restaurant in Jiangxi Province, China, was investigated. Cases were interviewed. Stool samples from cases, anal swabs from restaurant employees, suspicious raw food materials, and semifinished food were collected and examined. Pulsed-field gel electrophoresis (PFGE) and whole genome sequencing (WGS) were performed to determine the relatedness of the pathogen isolates. Antimicrobial resistance genes and virulence genes of isolates were analyzed by WGS. The antimicrobial profile of the isolates was detected by broth microdilution, which involved 20 different antibiotics. Among the 31 patrons, 26 showed gastrointestinal symptoms. Five <i>Salmonella</i> Enteritidis strains were isolated from patients (2), semifinished food (2), and food handler (1). The results of PFGE and single-nucleotide polymorphism showed that these five isolates were identical clones. These findings demonstrated that this outbreak was a restaurant <i>Salmonella</i> Enteritidis outbreak associated with an infected food handler. The rates of resistance to nalidixic acid and colistin and intermediate resistance to ciprofloxacin were 100%, 80%, and 100%, respectively. These outbreak isolates harbored point mutation <i>gyrA</i> p.D87G. The cause of inconsistency between the genotype and phenotype of resistance was deeply discussed. A total of 107 virulence genes were found in each isolate, with many being associated with <i>Salmonella</i> pathogenicity island (SPI)-1 and SPI-2. As an overlooked contamination source, infected food handlers can easily cause large-scale outbreaks. This outbreak highlighted that the government should enhance the training and supervision of food hygiene and safety for food handlers to prevent foodborne outbreaks.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"316-322"},"PeriodicalIF":2.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139734861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Characteristics of <i>Staphylococcus aureus</i> Isolates form Food-Poisoning Outbreaks (2011-2022) in Sichuan, China.","authors":"Jie Zheng, Li Liu, Guo Chen, Wenping Xu, Yulan Huang, Gaopeng Lei, Weifeng Huang, Hong Lv, Xiaorong Yang","doi":"10.1089/fpd.2023.0097","DOIUrl":"10.1089/fpd.2023.0097","url":null,"abstract":"<p><p>Staphylococcal food poisoning (SFP) is one of the most common foodborne diseases in the world. This study aimed to investigate the molecular epidemiological characteristics of <i>Staphylococcus aureus</i> isolated from SFP. A total of 103 <i>S. aureus</i> isolates were obtained during 2011-2022 in Sichuan, southwest China. All isolates were tested for the genomic characteristics and phylogenetic analysis by performing whole-genome sequencing. Multilocus sequence typing analysis showed 17 multilocus sequence types (STs), ST7 (23.30%), ST5 (22.33%), and ST6 (16.50%) being the most common. A total of 45 virulence genes were detected, 22 of which were staphylococcal enterotoxin (SE) genes. Among the identified SE genes, <i>selX</i> exhibited the highest prevalence (86.4%). All isolates carried at least one SE gene. The results of the antimicrobial resistance (AMR) gene detection revealed 41 AMR genes of 12 classes. β-lactam resistance genes (<i>blal</i>, <i>blaR1</i>, <i>blaZ</i>) and tetracycline resistance gene (<i>tet(38)</i>) exhibited a higher prevalence rate. Core genome single nucleotide polymorphism showed phylogenetic clustering of the isolates with the same region, year, and ST. The results indicated that the SFP isolates in southwest of China harbored multiple toxin and resistance genes, with a high prevalence of new SEs. Therefore, it is important to monitor the antimicrobial susceptibility and SE of <i>S. aureus</i> to reduce the potential risks to public health.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"323-330"},"PeriodicalIF":2.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139491137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Peptide Nucleic Acid Fluorescence <i>In Situ</i> Hybridization Probe for the Specific Detection of <i>Salmonella</i> Species in Food Matrices.","authors":"Mário Sousa, Rui Rocha, Daniela Araújo, Joana Castro, Ana Barbosa, Nuno F Azevedo, Laura Cerqueira, Carina Almeida","doi":"10.1089/fpd.2023.0127","DOIUrl":"10.1089/fpd.2023.0127","url":null,"abstract":"<p><p><i>Salmonella</i> spp. is among the most central etiological agents in foodborne bacterial disorders. To identify <i>Salmonella</i> spp., numerous new molecular techniques have been developed conversely to the traditional culture-based methods. In this work, a new peptide nucleic acid fluorescence <i>in situ</i> hybridization (PNA-FISH) method was developed for the specific detection of <i>Salmonella</i> species, allowing a faster analysis compared with the traditional methods (ISO 6579-1: 2017). The method was optimized based on a novel PNA probe (SalPNA1692) combined with a blocker probe to detect <i>Salmonell</i>a in food samples through an assessment of diverse-rich and selective enrichment broths. Our findings indicated that the best outcome was obtained using a 24-h pre-enrichment step in buffered peptone water, followed by RambaQuick broth selective enrichment for 16 h. For the enrichment step performance validation, fresh ground beef was artificially contaminated with two ranges of concentration of inoculum: a low level (0.2-2 colony-forming units [CFUs]/25 g) and a high level (2-10 CFUs/25 g). The new PNA-FISH method presented a specificity of 100% and a detection limit of 0.5 CFU/25 g of food sample, which confirms the great potential of applying PNA probes in food analysis.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"298-305"},"PeriodicalIF":2.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140131126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut Microbiota Protects <i>Listeria monocytogenes</i>-Infected Mice by Reducing the Inflammatory Cytokines Storm and Cell Apoptosis.","authors":"Liang Guo, Qing Liu, Xianhong Yin","doi":"10.1089/fpd.2023.0121","DOIUrl":"10.1089/fpd.2023.0121","url":null,"abstract":"<p><p>Gut microbiota (GM) has been proven to resist pathogenic infection through nutritional competition, colonization resistance and promotion of the host immune response. However, in clinical practice, GM is mainly used in intestinal diseases, such as <i>Clostridium difficile</i> infection, and there are few reports on its application in the treatment of pathogenic bacterial infections. In this study, GM from healthy mice was transplanted into mice infected with <i>Listeria monocytogenes</i> using fecal microbiota transplantation (FMT) and the effects were observed. We found that GM from healthy mice could reduce the mortality of infected mice and decrease the counts of <i>L. monocytogenes</i> in their liver and spleen. In addition, FMT inhibited the expression of inflammatory factors in the liver and spleen of infected mice. <i>In vitro</i> cell experiments revealed that GM can reduce the count of <i>L. monocytogenes</i> invading Caco-2 cells and inhibit the <i>L. monocytogenes</i>-caused apoptosis. These results indicate that GM can be used to protect mice infected with <i>L. monocytogenes</i> by eliminating the amount of <i>L. monocytogenes</i> in the host and inhibiting the overexpression of inflammatory factors. Hence, this method can potentially replace antibiotics in the treatment of <i>L. monocytogenes</i> infection.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"288-297"},"PeriodicalIF":2.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139491127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emulsified and Nanoemulsified Essential Oils in the Control of <i>Clostridium botulinum</i> and <i>Clostridium sporogenes</i> in Mortadella.","authors":"Juliana Junqueira Pinelli, Angélica Sousa Guimarães, Monique Suela Silva, Tatiana Silveira Junqueira de Moraes, Michelle Carlota Gonçalves, Roberta Hilsdorf Piccoli","doi":"10.1089/fpd.2023.0095","DOIUrl":"10.1089/fpd.2023.0095","url":null,"abstract":"<p><p><i>Clostridium botulinum</i> is a foodborne pathogen responsible for severe neuroparalytic disease associated with the ingestion of pre-formed toxin in food, with processed meats and canned foods being the most affected. Control of this pathogen in meat products is carried out using the preservative sodium nitrite (NaNO₂), which in food, under certain conditions, such as thermal processing and storage, can form carcinogenic compounds. Therefore, the objective was to use nanoemulsified essential oils (EOs) as natural antimicrobial agents, with the aim of reducing the dose of NaNO₂ applied in mortadella. The antimicrobial activity of nanoemulsions prepared with mixtures of EOs of garlic, clove, pink pepper, and black pepper was evaluated on endospores and vegetative cells of <i>C. botulinum</i> and <i>Clostridium sporogenes</i> (surrogate model) inoculated in mortadella prepared with 50 parts per million NaNO₂. The effects on the technological (pH, water activity, and color) and sensory characteristics of the product were also evaluated. The combinations of EOs and their nanoemulsions showed sporicidal effects on the endospores of both tested microorganisms, with no counts observed from the 10th day of analysis. Furthermore, bacteriostatic effects on the studied microorganisms were observed. Regarding the technological and sensorial characteristics of the product, the addition of the combined EOs had a negative impact on the color of the mortadella and on the flavor/aroma. Despite the strong commercial appeal of adding natural preservatives to foods, the effects on flavor and color must be considered. Given the importance of controlling <i>C. botulinum</i> in this type of product, as well as the reduction in the amount of NaNO₂ used, this combination of EOs represents a promising antimicrobial alternative to this preservative, encouraging further research in this direction.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"339-352"},"PeriodicalIF":2.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139996058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of Pathogenic <i>Escherichia coli</i> Attachment to Meat.","authors":"Nicolae Corcionivoschi, Igori Balta, David McCleery, Iulia Bundurus, Ioan Pet, Todd Calaway, Ileana Nichita, Lavinia Stef, Sorin Morariu","doi":"10.1089/fpd.2023.0164","DOIUrl":"https://doi.org/10.1089/fpd.2023.0164","url":null,"abstract":"<p><p><i>Escherichia coli</i> are present in the human and animal microbiome as facultative anaerobes and are viewed as an integral part of the whole gastrointestinal environment. In certain circumstances, some species can also become opportunistic pathogens responsible for severe infections in humans. These infections are caused by the enterotoxinogenic <i>E. coli</i>, enteroinvasive <i>E. coli</i>, enteropathogenic <i>E. coli</i> and the enterohemorrhagic <i>E. coli</i> species, frequently present in food products and on food matrices. Severe human infections can be caused by consumption of meat contaminated upon exposure to animal feces, and as such, farm animals are considered to be a natural reservoir. The mechanisms by which these four major species of <i>E. coli</i> adhere and persist in meat postslaughter are of major interest to public health and food processors given their frequent involvement in foodborne outbreaks. This review aims to structure and provide an update on the mechanistic roles of environmental factors, curli, type I and type IV pili on <i>E. coli</i> adherence/interaction with meat postslaughter. Furthermore, we emphasize on the importance of bacterial surface structures, which can be used in designing interventions to enhance food safety and protect public health by reducing the burden of foodborne illnesses.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140874574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Application of Four Foodborne Pathogens by TaqMan Multiplex Real-Time PCR.","authors":"Yinlei Xue, Shengfang He, Meng Li, Yuanhao Qiu","doi":"10.1089/fpd.2023.0134","DOIUrl":"https://doi.org/10.1089/fpd.2023.0134","url":null,"abstract":"<p><p>A TaqMan multiplex real-time PCR (mRT-PCR) was developed to detect simultaneously <i>Salmonella</i> spp., <i>Escherichia coli</i> O157, <i>Staphylococcus aureus</i>, and <i>Listeria monocytogene</i>s in food samples. The method involves four sets of primers and probes tailored to the unique DNA sequences found in the <i>invA</i>, <i>nuc</i>, <i>rfbE</i>, and <i>hly</i> genes of each pathogen. The generated standard curves, correlating gene copy numbers with Ct values, demonstrated high accuracy (<i>R</i>² > 0.99) and efficiency (92%-104%). Meanwhile, the limit of detection was 10⁰ CFU/mL for the four target bacteria in artificially contaminated food samples after 6-8 h of enrichment. The assay's effectiveness was further verified by testing 80 naturally contaminated food samples, showing results largely in agreement with traditional culture methods. Overall, this newly developed TaqMan mRT-PCR, inclusive of a pre-enrichment step, proves to be a dependable and effective tool for detecting single or multiple pathogens in diverse food items, offering significant potential for <i>in vitro</i> diagnostics.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Resistance Profiles of <i>Campylobacter</i> spp. Recovered from Chicken Farms in Two Districts of Bangladesh.","authors":"Mehedi Hasan, Sudipta Talukder, Amit Kumar Mandal, Syeda Tanjina Tasmim, Sonia Parvin, Yamin Ali, Mahmudul Hasan Sikder, Thomas J Callaghan, Ricardo J Soares Magalhães, Taohidul Islam","doi":"10.1089/fpd.2023.0079","DOIUrl":"https://doi.org/10.1089/fpd.2023.0079","url":null,"abstract":"<p><p>The rapid emergence of antimicrobial resistance (AMR) in <i>Campylobacter</i> has reinforced its status as a foodborne pathogen of significant public health concern. Resistant <i>Campylobacter</i> is typically transferred to humans via the consumption of contaminated animal products, particularly poultry. The genes associated with antimicrobial resistance in <i>Campylobacter</i> spp. are poorly understood. To address this knowledge gap, we conducted a prevalence survey of AMR <i>Campylobacter</i> across 84 chicken farms in two districts of Bangladesh. Pooled cloacal swabs were collected from chickens and underwent bacteriological testing for <i>Campylobacter</i> spp. with PCR confirmation. Antimicrobial susceptibility was tested against 14 antibiotics by disk diffusion method, and 12 resistance genes were screened in <i>Campylobacter</i>-positive isolates using multiplex PCR. A total of 34 (40.5%) farms were <i>Campylobacter</i>-positive of which 73.5% of isolates were resistant to at least 10 antibiotics. The antimicrobial susceptibility results indicate a high level of resistance against streptomycin (97.1%), clindamycin (97.1%), ampicillin (94.1%), tetracycline (94.1%), erythromycin (91.2%), ciprofloxacin (88.2%), nalidixic acid (85.3%), and imipenem (82.4%), and comparatively a low frequency of resistance to chloramphenicol (47.1%), ceftazidime (44.1%), and colistin (35.3%). Multidrug-resistant (MDR) and extensively drug-resistant <i>Campylobacter</i> were identified in 97.1%, and 50% of isolates, respectively. Ten resistance genes were identified including <i>bla</i>_TEM (in 97.1% of isolates), <i>strA</i>-<i>strB</i> (85.9%), <i>tetA</i> (70.6%), <i>tetB</i> (32.4%), <i>qnrS</i> (23.5%), <i>bla</i>_CTX-M-1 (20.6%), <i>qnrB</i> (20.6%), <i>bla</i>_SHV (8.8%), <i>aadB</i> (5.9%), and <i>qnrA</i> (2.9%). Our findings demonstrate that resistance to ampicillin, tetracycline, and ceftazidime in <i>Campylobacter</i> isolates was significantly (<i>p</i> ≤ 0.05) associated with the presence of <i>bla</i>_TEM, <i>tetA</i>, and <i>bla</i>_SHV genes, respectively. The high rates of AMR in <i>Campylobacter</i> isolates from our study are not surprising given the liberal use of antimicrobials and incomplete biosecurity provisions on farms. Of particular concern are resistance rates to those classes of antibiotics that should be reserved for human use (azithromycin, ciprofloxacin, and colistin). AMR was more prevalent in chicken farms that used multiple antibiotics, engaged in prophylactic treatment of the birds, and improperly disposed of antibiotic packages. The high prevalence of MDR in chicken-derived <i>Campylobacter</i> isolates from the different regions of our study reinforces the need for more prudent use of antimicrobial compounds in Bangladeshi chicken farms.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcontinental Dissemination of Enterobacterales Harboring <i>bla</i>_NDM-1 in Retail Frozen Shrimp.","authors":"Elizabeth M Parker, Dixie F Mollenkopf, Gregory A Ballash, Cong Li, Thomas E Wittum","doi":"10.1089/fpd.2023.0161","DOIUrl":"https://doi.org/10.1089/fpd.2023.0161","url":null,"abstract":"<p><p>The global food trade provides a means of disseminating antimicrobial resistant (AMR) bacteria and genes. Using selective media, carbapenem-resistant species of Enterobacterales (<i>Providencia</i> sp. and <i>Citrobacter</i> sp.), were detected in a single package of imported frozen shrimp purchased from a grocery store in Ohio, USA. Polymerase chain reaction confirmed that both isolates harbored <i>bla</i>_NDM-1 genes. Following PacBio long read sequencing, the sequences were annotated using the NCBI Prokaryotic Genome Annotation Pipeline. The <i>bla</i>_NDM-1 genes were found in IncC plasmids, each with different antimicrobial resistance island configuration. We found that the <i>bla</i>_NDM-1 AMR islands had close relationships with previously reported environmental, food, and clinical isolates detected in Asia and the United States, highlighting the importance of the food chain in the global dissemination of antimicrobial resistance.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}