Broad-Spectrum Antimicrobial Peptides Suppress Vibrio parahaemolyticus Based on Lactobacillus paracasei A1 Fermentation.

IF 1.9 2区 农林科学 Q3 FOOD SCIENCE & TECHNOLOGY
Feng Guan, Chen Yu, Lu Yang, Yongjun Yuan
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Abstract

Growing evidence supports the efficacy of antimicrobial peptides against foodborne pathogens, though their antimicrobial spectrum and mechanism can vary depending on their origin. We investigated the antimicrobial spectrum of antimicrobial peptides derived from Lactobacillus paracasei A1, their effects on the survival rate and bactericidal mechanisms against Vibrio parahaemolyticus, and identified the functional short peptides within them. The crude extracts of antimicrobial peptides exhibited antibacterial properties against 13 pathogenic bacteria, showing strong inhibition of V. parahaemolyticus by disrupting the structural integrity of cell membranes. At minimum inhibitory concentrations, these peptides significantly disrupted the initial adhesion, membrane formation, and existing biofilms of V. parahaemolyticus, effectively inhibiting pathogen spread, enhancing the efficacy of antimicrobial agents, and reducing food safety risks. LC-MS/MS identification revealed four effective short peptides, all demonstrating potent bacteriostatic effects against V. parahaemolyticus. Our findings indicate that antimicrobial peptides can effectively destroy bacterial structures as well as the stability and regeneration of biofilms, making them promising candidates for use as food additives to control foodborne pathogens.

基于副干酪乳杆菌A1发酵的广谱抗菌肽抑制副溶血性弧菌。
越来越多的证据支持抗菌肽对食源性病原体的有效性,尽管它们的抗菌谱和机制可能因来源而异。研究了从副干酪乳杆菌A1提取的抗菌肽的抗菌谱,对其对副溶血性弧菌存活率的影响及抗菌机制,并鉴定了其中的功能性短肽。抗菌肽粗提物对13种病原菌具有抗菌作用,通过破坏细胞膜结构完整性对副溶血性弧菌有较强的抑制作用。在最低抑制浓度下,这些肽显著破坏副溶血性弧菌的初始粘附、膜形成和现有生物膜,有效抑制病原体传播,增强抗菌药物的功效,降低食品安全风险。LC-MS/MS鉴定发现了四种有效的短肽,均显示出对副溶血性弧菌的有效抑菌作用。我们的研究结果表明,抗菌肽可以有效地破坏细菌结构,以及生物膜的稳定性和再生,使其成为控制食源性病原体的食品添加剂的有希望的候选人。
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来源期刊
Foodborne pathogens and disease
Foodborne pathogens and disease 医学-食品科技
CiteScore
5.30
自引率
3.60%
发文量
80
审稿时长
1 months
期刊介绍: Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.
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