Assessment of Antimicrobial Exposure on Generic Escherichia coli and Enterococcus spp. Concentration, Prevalence, and Resistance to Antimicrobials in Beef Cattle Raised with or Without Antimicrobials.

IF 1.9 2区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY

Foodborne pathogens and disease Pub Date : 2025-02-07 DOI:10.1089/fpd.2024.0145

Colten W Dornbach, James E Wells, Elaine D Berry, Samodha C Fernando, Paul R Broadway, Kristin E Hales

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Abstract

The aim was to longitudinally evaluate the association between antimicrobial exposure and resistance occurrence within generic Escherichia coli and Enterococcus spp. populations in feedlot beef cattle raised conventionally or raised without antimicrobials. Angus steers (n = 180) were sorted into 1 of 2 treatments over 2 consecutive years (108 in yr 1 and 72 in yr 2): steers raised without antimicrobials (NAT) and conventionally raised steers exposed to antimicrobials (CONV). Pens within treatment were adjacent and separated by five empty pens from the other treatment. Monensin and tylosin were included in CONV steer diets. On d 123, CONV steers received a metaphylactic antimicrobial. Longitudinal diet (n = 6/year) and fecal (n = 5/year) sampling timepoints were collected to determine E. coli and Enterococcus spp. concentration, prevalence, and resistance patterns. Dietary Enterococcus spp. concentrations, and erythromycin (8ERY^R; 128ERY^R), tetracycline (TET^R), trimethoprim-sulfamethoxazole (COT^R), and cefotaxime (CTX^R) resistant E. coli concentrations and prevalence were greater in NAT diets than CONV diets (p < 0.02). Fecal E. coli concentrations tended to be greater in NAT steers than CON steers (p = 0.07). Fecal TET^R E. coli concentrations were greater in CONV steers than NAT steers (p = 0.03). Fecal COT^R and CTX^R E. coli prevalence was greater for CONV steers at the beginning of the finishing phase while greater for NAT steers at the end of the finishing phase (p < 0.01). Fecal Enterococcus spp. concentrations did not differ between treatments (p = 0.11). Concentrations of 8ERY^R and 128ERY^R Enterococcus spp. were greater in CONV steers on d 64, 130, and 168 than NAT steers (p < 0.05). Overall, antimicrobial resistant Enterococcus spp. and E. coli were detected regardless of antimicrobial exposure.

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在使用或不使用抗菌剂饲养的肉牛中，对通用大肠杆菌和肠球菌的抗生素暴露评估。浓度、流行率和对抗菌剂的耐药性。

目的是纵向评估在常规饲养或不使用抗菌剂饲养的饲养场肉牛中，普通大肠杆菌和肠球菌种群中抗生素暴露与耐药性之间的关系。180只安格斯阉牛（n = 180）在连续2年的时间里被分为2种处理中的1种（1年108只，2年72只）：不使用抗菌剂饲养的阉牛（NAT）和常规饲养的使用抗菌剂饲养的阉牛（CONV）。处理内的笔是相邻的，并与其他处理的5只空笔分开。在CONV阉牛日粮中添加莫能菌素和泰络菌素。在第123天，CONV患者接受了过敏性抗菌药物治疗。收集纵向饮食（n = 6/年）和粪便（n = 5/年）采样时间点，以确定大肠杆菌和肠球菌的浓度、患病率和耐药模式。饲粮中肠球菌浓度、红霉素(8ERYR；对128ERYR)、四环素（TETR）、甲氧苄啶-磺胺甲恶唑（COTR）和头孢噻肟（CTXR）耐药的大肠杆菌浓度和患病率在NAT饲粮中高于CONV饲粮（p < 0.02）。NAT组的粪便大肠杆菌浓度高于CON组（p = 0.07）。CONV组粪便中TETR大肠杆菌浓度高于NAT组（p = 0.03）。COTR和CTXR大肠杆菌在育肥期开始时和在育肥期结束时均高于对照组（p < 0.01）。粪肠球菌浓度在不同处理间无差异（p = 0.11）。在第64、130和168天，CONV组的8ERYR和128ERYR肠球菌浓度高于NAT组（p < 0.05）。总体而言，无论是否暴露于抗菌素，均检测到耐药肠球菌和大肠杆菌。

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来源期刊

Foodborne pathogens and disease 医学-食品科技

CiteScore

5.30

自引率

3.60%

发文量

审稿时长

1 months

期刊介绍： Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.