Foodborne pathogens and disease最新文献

{"title":"Development and Application of Four Foodborne Pathogens by TaqMan Multiplex Real-Time PCR.","authors":"Yinlei Xue, Shengfang He, Meng Li, Yuanhao Qiu","doi":"10.1089/fpd.2023.0134","DOIUrl":"https://doi.org/10.1089/fpd.2023.0134","url":null,"abstract":"<p><p>A TaqMan multiplex real-time PCR (mRT-PCR) was developed to detect simultaneously <i>Salmonella</i> spp., <i>Escherichia coli</i> O157, <i>Staphylococcus aureus</i>, and <i>Listeria monocytogene</i>s in food samples. The method involves four sets of primers and probes tailored to the unique DNA sequences found in the <i>invA</i>, <i>nuc</i>, <i>rfbE</i>, and <i>hly</i> genes of each pathogen. The generated standard curves, correlating gene copy numbers with Ct values, demonstrated high accuracy (<i>R</i>² > 0.99) and efficiency (92%-104%). Meanwhile, the limit of detection was 10⁰ CFU/mL for the four target bacteria in artificially contaminated food samples after 6-8 h of enrichment. The assay's effectiveness was further verified by testing 80 naturally contaminated food samples, showing results largely in agreement with traditional culture methods. Overall, this newly developed TaqMan mRT-PCR, inclusive of a pre-enrichment step, proves to be a dependable and effective tool for detecting single or multiple pathogens in diverse food items, offering significant potential for <i>in vitro</i> diagnostics.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Resistance Profiles of <i>Campylobacter</i> spp. Recovered from Chicken Farms in Two Districts of Bangladesh.","authors":"Mehedi Hasan, Sudipta Talukder, Amit Kumar Mandal, Syeda Tanjina Tasmim, Sonia Parvin, Yamin Ali, Mahmudul Hasan Sikder, Thomas J Callaghan, Ricardo J Soares Magalhães, Taohidul Islam","doi":"10.1089/fpd.2023.0079","DOIUrl":"https://doi.org/10.1089/fpd.2023.0079","url":null,"abstract":"<p><p>The rapid emergence of antimicrobial resistance (AMR) in <i>Campylobacter</i> has reinforced its status as a foodborne pathogen of significant public health concern. Resistant <i>Campylobacter</i> is typically transferred to humans via the consumption of contaminated animal products, particularly poultry. The genes associated with antimicrobial resistance in <i>Campylobacter</i> spp. are poorly understood. To address this knowledge gap, we conducted a prevalence survey of AMR <i>Campylobacter</i> across 84 chicken farms in two districts of Bangladesh. Pooled cloacal swabs were collected from chickens and underwent bacteriological testing for <i>Campylobacter</i> spp. with PCR confirmation. Antimicrobial susceptibility was tested against 14 antibiotics by disk diffusion method, and 12 resistance genes were screened in <i>Campylobacter</i>-positive isolates using multiplex PCR. A total of 34 (40.5%) farms were <i>Campylobacter</i>-positive of which 73.5% of isolates were resistant to at least 10 antibiotics. The antimicrobial susceptibility results indicate a high level of resistance against streptomycin (97.1%), clindamycin (97.1%), ampicillin (94.1%), tetracycline (94.1%), erythromycin (91.2%), ciprofloxacin (88.2%), nalidixic acid (85.3%), and imipenem (82.4%), and comparatively a low frequency of resistance to chloramphenicol (47.1%), ceftazidime (44.1%), and colistin (35.3%). Multidrug-resistant (MDR) and extensively drug-resistant <i>Campylobacter</i> were identified in 97.1%, and 50% of isolates, respectively. Ten resistance genes were identified including <i>bla</i>_TEM (in 97.1% of isolates), <i>strA</i>-<i>strB</i> (85.9%), <i>tetA</i> (70.6%), <i>tetB</i> (32.4%), <i>qnrS</i> (23.5%), <i>bla</i>_CTX-M-1 (20.6%), <i>qnrB</i> (20.6%), <i>bla</i>_SHV (8.8%), <i>aadB</i> (5.9%), and <i>qnrA</i> (2.9%). Our findings demonstrate that resistance to ampicillin, tetracycline, and ceftazidime in <i>Campylobacter</i> isolates was significantly (<i>p</i> ≤ 0.05) associated with the presence of <i>bla</i>_TEM, <i>tetA</i>, and <i>bla</i>_SHV genes, respectively. The high rates of AMR in <i>Campylobacter</i> isolates from our study are not surprising given the liberal use of antimicrobials and incomplete biosecurity provisions on farms. Of particular concern are resistance rates to those classes of antibiotics that should be reserved for human use (azithromycin, ciprofloxacin, and colistin). AMR was more prevalent in chicken farms that used multiple antibiotics, engaged in prophylactic treatment of the birds, and improperly disposed of antibiotic packages. The high prevalence of MDR in chicken-derived <i>Campylobacter</i> isolates from the different regions of our study reinforces the need for more prudent use of antimicrobial compounds in Bangladeshi chicken farms.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcontinental Dissemination of Enterobacterales Harboring <i>bla</i>_NDM-1 in Retail Frozen Shrimp.","authors":"Elizabeth M Parker, Dixie F Mollenkopf, Gregory A Ballash, Cong Li, Thomas E Wittum","doi":"10.1089/fpd.2023.0161","DOIUrl":"https://doi.org/10.1089/fpd.2023.0161","url":null,"abstract":"<p><p>The global food trade provides a means of disseminating antimicrobial resistant (AMR) bacteria and genes. Using selective media, carbapenem-resistant species of Enterobacterales (<i>Providencia</i> sp. and <i>Citrobacter</i> sp.), were detected in a single package of imported frozen shrimp purchased from a grocery store in Ohio, USA. Polymerase chain reaction confirmed that both isolates harbored <i>bla</i>_NDM-1 genes. Following PacBio long read sequencing, the sequences were annotated using the NCBI Prokaryotic Genome Annotation Pipeline. The <i>bla</i>_NDM-1 genes were found in IncC plasmids, each with different antimicrobial resistance island configuration. We found that the <i>bla</i>_NDM-1 AMR islands had close relationships with previously reported environmental, food, and clinical isolates detected in Asia and the United States, highlighting the importance of the food chain in the global dissemination of antimicrobial resistance.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Cymbopogon citratus</i> Essential Oil in Controlling of Bacteria Associated with Oral Cavity of Dogs <i>In Vitro</i> and Dry Pet Feed.","authors":"Marco Aurélio Cunha Del Vechio, Karolaine Bezerra, Jocimara Estevam Dos Santos, Jacqueline Midori Ono, Isabela Carvalho Dos Santos, Daniela Dib Gonçalves, Lidiane Nunes Barbosa","doi":"10.1089/fpd.2023.0109","DOIUrl":"10.1089/fpd.2023.0109","url":null,"abstract":"<p><p>The aim of this study was to evaluate the antibacterial activity of <i>Cymbopogon citratus</i> essential oil against bacteria isolated from the oral cavity of dogs and applied directly to artificially contaminated feed. The commercial dry dog feed received the inoculum of the bacteria prevalent in the oral cavity of the dogs where <i>C. citratus</i> was deposited in the feed by spray. In total, 42 isolates were obtained, 38 Gram-positive and 4 Gram-negative. <i>Staphylococcus</i> spp. was the most prevalent bacteria in the oral cavity of dogs (76.2%). The isolates showed high levels of resistance to different antimicrobials. The minimum inhibitory concentration capable of inhibiting oral bacteria was 0.38 mg/mL. <i>C. citratus</i> essential oil showed positive results with a significant bacterial reduction when applied to feed, signaling the need for further studies to enable its use in the control of pathogens transmitted by animal feed.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139477999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clostridiales in the Gut Against <i>Listeria monocytogenes</i> Infection Through Growth Inhibition.","authors":"Liang Guo, Qing Liu, Xianhong Yin","doi":"10.1089/fpd.2023.0049","DOIUrl":"10.1089/fpd.2023.0049","url":null,"abstract":"<p><p><i>Listeria monocytogenes</i> (Lm) mainly infect pregnant women, children, the elderly, and other populations with low immunity causing septicemia and meningitis. Healthy people can tolerate higher doses of Lm and only cause gastrointestinal symptoms such as abdominal pain and diarrhea after infection. Compared to the above population, healthy people have a richer and more diverse gut microbiota. In this study, we show that the microbiota in the large intestine and the feces of mice can significantly inhibit the growth of Lm compared to the microbiota in the small intestine. Bacteria larger than 1 μm in the gut microbiota play an important role in inhibiting Lm growth. 16s rRNA sequencing results show that these bacteria are mainly composed of Clostridiales under the phylum Firmicutes, including <i>Ruminiclostridium</i>, <i>Butyricicoccus</i>, <i>Lachnoclostridium</i>, <i>Roseburia</i>, <i>Coprooccus</i>, and <i>Blautia</i>. Thus, we demonstrate that there are some potential functional bacteria in the gut microbiota that can increase resistance against Lm.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139039721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Foodborne Pathogen and Microbial Community Differences in Fresh Processing Tomatoes in Xinjiang, China.","authors":"Shicui Li, Yingying Fan, Jie Han, Fengjuan Liu, Yu Ding, Xiaolong Li, Enhe Yu, Shuai Wang, Fulan Wang, Cheng Wang","doi":"10.1089/fpd.2023.0014","DOIUrl":"10.1089/fpd.2023.0014","url":null,"abstract":"<p><p>The microbes on fresh processing tomatoes correlate closely with diseases, preservation, and quality control. Investigation of the microbial communities on processing tomatoes from different production regions may help define microbial specificity, inform disease prevention methods, and improve quality. In this study, surface microbes on processing tomatoes from 10 samples in two primary production areas of southern and northern Xinjiang were investigated by sequencing fungal internal transcribed spacer and bacterial 16S rRNA hypervariable sequences. A total of 133 different fungal and bacterial taxonomies were obtained from processing tomatoes in the two regions, of which 63 genera were predominant. Bacterial and fungal communities differed significantly between southern and northern Xinjiang, and fungal diversity was higher in southern Xinjiang. <i>Alternaria</i> and <i>Cladosporium</i> on processing tomatoes in southern Xinjiang were associated with plant pathogenic risk. The plant pathogenic fungi of processing tomatoes in northern Xinjiang were more abundant in <i>Alternaria</i> and <i>Fusarium</i>. The abundance of <i>Alternaria</i> on processing tomatoes was higher in four regions of northern Xinjiang, indicating that there is a greater risk of plant pathogenicity in these areas. Processing tomatoes in northern and southern Xinjiang contained bacterial genera identified as gut microbes, such as <i>Pantoea</i>, <i>Erwinia</i>, <i>Enterobacter</i>, <i>Enterococcus</i>, and <i>Serratia</i>, indicating the potential risk of contamination of processing tomatoes with foodborne pathogens. This study highlighted the microbial specificity of processing tomatoes in two tomato production regions, providing a basis for further investigation and screening for foodborne pathogenic microorganisms.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139039722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of <i>Salmonella</i> Outer Membrane Vesicles on Intestinal Microbiota and Intestinal Barrier Function.","authors":"Meiying Luo, Suqian Li, Yang Yang, Junhang Sun, Yuman Su, Dechun Huang, Xin Feng, Huihua Zhang, Qien Qi","doi":"10.1089/fpd.2023.0096","DOIUrl":"10.1089/fpd.2023.0096","url":null,"abstract":"<p><p><i>Salmonella enterica</i> is one of the most important zoonotic pathogens causing foodborne gastroenteritis worldwide. Outer membrane vesicles (OMVs) are lipid-bilayer vesicles produced by Gram-negative bacteria, which contain biologically active components. We hypothesized that OMVs are an important weapon of <i>S. enterica</i> to initiate enteric diseases pathologies. In this study, the effects of <i>S. enterica</i> OMVs (<i>Se</i>OMVs) on intestinal microbiota and intestinal barrier function were investigated. <i>In vitro</i> fecal culture experiments showed that alpha diversity indexes and microbiota composition were altered by <i>Se</i>OMV supplementation. <i>Se</i>OMV supplementation showed an increase of pH, a decrease of OD630 and total short chain fatty acid (SCFA) concentrations. <i>In vitro</i> IPEC-J2 cells culture experiments showed that <i>Se</i>OMV supplementation did not affect the IPEC-J2 cell viability and the indicated genes expression. <i>In vivo</i> experiments in mice showed that <i>Se</i>OMVs had adverse effects on average daily gain (<i>p</i> < 0.05) and feed:gain ratio (<i>p</i> < 0.05), and had a tendency to decrease the final body weight (<i>p</i> = 0.073) in mice. <i>Se</i>OMV administration decreased serum interleukin-10 level (<i>p</i> < 0.05), decreased the relative abundance of bacteria belonging to the genera <i>BacC-u</i>-018 and <i>Akkermansia</i> (<i>p</i> < 0.05). Furthermore, <i>Se</i>OMV administration damaged the ileum mucosa (<i>p</i> < 0.05). These findings suggest that <i>Se</i>OMVs play an important role in the activation of intestinal inflammatory response induced by <i>S. enterica</i>, and downregulation of SCFA-producing bacteria is a possible mechanism.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139432391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Rosa roxburghii</i> Tratt Pomace Crude Extract Inactivates <i>Cronobacter sakazakii</i> Isolated from Powdered Infant Formula.","authors":"Peng Fei, Jing Xu, Jinlan Xie, Jicheng Huang, Hongxia Feng, Xi Chen, Peiyi Jiang, Mingliang Guo, Yunhe Chang","doi":"10.1089/fpd.2023.0088","DOIUrl":"10.1089/fpd.2023.0088","url":null,"abstract":"<p><p><i>Cronobacter sakazakii</i> is an important foodborne pathogen in powder infant formula (PIF). The objective of this study was to evaluate the inactivation effect of <i>Rosa roxburghii</i> Tratt pomace crude extract (RRPCE) on <i>C. sakazakii</i> isolated from PIF and to reveal the mechanism of action. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were used to evaluate the inhibitory activity of RRPCE against <i>C. sakazakii</i>. The inhibitory mechanism was revealed from the perspective of effects of RRPCE on intracellular adenosine 5'-triphosphate (ATP), reactive oxygen species (ROS), membrane potential, protein and nucleic acid leakage, and cell morphology of <i>C. sakazakii</i>. The inactivation effects of RRPCE on <i>C. sakazakii</i> in biofilms on stainless steel, tinplate, glass, silica gel, polyethylene terephthalate, and polystyrene to evaluate its potential as a natural disinfectant. The results showed that the MIC and MBC of RRPCE against <i>C. sakazakii</i> were 7.5 and 15 mg/mL, respectively. After treatments with RRPCE, intracellular ATP content decreased significantly while intracellular ROS level increased significantly (<i>p</i> < 0.05). The cell membrane depolarization, large leakage of proteins and nucleic acids, and severely damaged cell morphology also occurred in <i>C. sakazakii</i> treated with RRPCE. In addition, a 20-minute treatment with 2 MIC (15 mg/mL) of RRPCE could inactivate all <i>C. sakazakii</i> (from 6.10 to 6.40 CFU/mL) in biofilms on all six contact surfaces. Our findings suggest that RRPCE is ideal for the inactivation of <i>C. sakazakii</i> and has the potential to be used as a natural disinfectant for the inactivation of PIF packaging materials and containers.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139541973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic and Genotypic Characterizations of Antimicrobial-Resistant <i>Escherichia coli</i> Isolates from Diverse Retail Meat Samples in North Carolina During 2018-2019.","authors":"Afaf Abdelrahim, Erin Harrell, Paula J Fedorka-Cray, Megan Jacob, Siddhartha Thakur","doi":"10.1089/fpd.2023.0093","DOIUrl":"10.1089/fpd.2023.0093","url":null,"abstract":"<p><p>Surveillance of antimicrobial-resistant pathogens in U.S. retail meats is conducted to identify potential risks of foodborne illness. In this study, we conducted a phenotypic and genotypic analysis of <i>Escherichia coli</i> recovered from a diverse range of retail meat types during 2018-2019 in North Carolina. The investigation was conducted as part of the National Antimicrobial Resistance Monitoring System (NARMS). Retail meat sampling and <i>E. coli</i> isolation were performed in accordance with NARMS retail meat isolation protocols. We used the Sensititre™ broth microdilution system to determine phenotypic resistance to 14 antimicrobial agents and the Illumina next-generation sequencing platform for genotypic resistance profiling. The highest prevalence of <i>E. coli</i> isolates was found in ground turkey (<i>n</i> = 57, 42.9%) and chicken (<i>n</i> = 27, 20.3%), followed by ground beef (<i>n</i> = 25, 18.9%) and pork (<i>n</i> = 24, 18%). The isolates were divided into seven different phylogroups using the Clermont typing tool, with B1 (<i>n</i> = 59, 44.4%) and A (<i>n</i> = 39, 29.3%) being the most dominant, followed by B2 (<i>n</i> = 14, 10.5%), D (<i>n</i> = 7, 5.3%), F (<i>n</i> = 6, 4.5%), E (<i>n</i> = 3, 2.3%), and C (<i>n</i> = 2, 1.5%). Using multilocus sequence typing (MLST), 128 Sequence types (STs) were identified indicating high diversity. Phenotypic and genotypic resistance was observed toward aminoglycosides, sulfonamides, beta-lactams, macrolides, tetracyclines, phenicols, and fluoroquinolones. Ground turkey samples were more resistant to the panel of tested antimicrobials than chicken, beef, or pork (<i>p</i> < 0.05). All isolates were found to be susceptible to meropenem. A high percentage of turkey isolates (<i>n</i> = 16, 28%) were multidrug-resistant (MDR) compared with 18.5% of chicken (<i>n</i> = 5), 8.4% of pork (<i>n</i> = 2), and 8% of beef isolates (<i>n</i> = 2). This study highlights the benefit of surveillance to identify MDR <i>E. coli</i> for epidemiologic tracking and is a comprehensive report of the phenotypic and genotypic characterization of <i>E. coli</i> isolated from retail meats in North Carolina.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139402549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Foodborne Outbreak by <i>Salmonella enterica</i> Serovar Weltevreden in West Bengal, India.","authors":"Goutam Chowdhury, Falguni Debnath, Mainak Bardhan, Alok Kumar Deb, Rama Bhuina, Sudipta Bhattacharjee, Koushik Mondal, Kei Kitahara, Shin-Ichi Miyoshi, Shanta Dutta, Asish K Mukhopadhyay","doi":"10.1089/fpd.2023.0064","DOIUrl":"10.1089/fpd.2023.0064","url":null,"abstract":"<p><p>Foodborne gastroenteritis outbreaks owing to <i>Salmonella enterica</i> serovar Weltevreden (<i>Salmonella</i> Weltevreden) represent a significant global public health problem. In the past two decades, <i>Salmonella</i> Weltevreden has emerged as a dominant foodborne pathogen, especially in South-East Asian countries. This report describes a community foodborne outbreak of gastroenteritis caused by <i>Salmonella</i> Weltevreden in August 2022 following consumption of panipuri from a street vendor in the Polba block in Hooghly district, West Bengal, India. This food item was consumed by 185 people, of whom 129 had acute watery diarrhea with other clinical symptoms and 65 of them were admitted to different District hospitals for treatment. Stool specimens collected from hospitalized cases were positive for <i>S. enterica</i>, and further serotyped as <i>Salmonella</i> Weltevreden. All the <i>Salmonella</i> Weltevreden strains possessed the <i>Salmonella</i> pathogenicity islands associated genes (<i>invA/E</i>, <i>orgA</i>, <i>ttrc</i>, <i>ssaQ</i>, <i>mgtC</i>, <i>misL</i>, <i>spi4D)</i>, the enterotoxin (<i>stn</i>), and hyperinvasive locus gene (<i>hilA</i>). Except erythromycin, all the strains were susceptible for commonly used antimicrobials in the treatment of diarrhea. The <i>Xba</i>I-based pulsed-field gel electrophoresis analysis indicated that all the isolates responsible for the recent outbreak were similar, but diverged from other <i>Salmonella</i> Weltevreden that were previously reported in West Bengal. This report indicates that foodborne infection is a major public health concern in India and demands to strengthen capacity-building measures at the local health care levels for linking causative agents of outbreaks.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139402548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}