Foodborne pathogens and disease最新文献

{"title":"Investigation of Biofilm Formation Ability and Antibiotic Resistance of <i>Staphylococcus aureus</i> Isolates from Food Products.","authors":"Yacine Titouche, Madjid Akkou, Yasmina Djaoui, Achour Chergui, Donia Mechoub, Lamia Bentayeb, Abdelhak Fatihi, Yacine Nia, Jacques-Antoine Hennekinne","doi":"10.1089/fpd.2024.0121","DOIUrl":"10.1089/fpd.2024.0121","url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> is one of the major causes of foodborne diseases and its presence in food products may poses a public health challenge. The aims of this study were to assess <i>in vitro</i> the capacity of <i>S. aureus</i> isolates from foods to form biofilm and to determine their antibiotic susceptibility. A total of 80 <i>S. aureus</i> isolates were characterized. The slime production ability was evaluated by congo-red agar (CRA) and the biofilm formation was carried out by microtiter-plate method (MPM). Resistance of isolates to eight antibiotics was determined using disc diffusion method. Sixty-four (80%) of the isolates were slime producers on congo-red agar. However, all isolates were biofilm producers on microtiter-plate method. The highest resistance profiles were ascribed to penicillin G (91.25%) and tetracycline (41.25%). Twelve isolates were methicillin-resistant (MRSA) harboring the <i>mecA</i> gene. All of these MRSA isolates were negative for the genes of the Panton Valentine leukocidine (<i>lukF/S</i>-PV). Typing of the MRSA isolates indicated that they belonged to three spa-types including t024, t450 and t688. The presence of biofilm producers and multidrug resistant isolates (MRSA) in food samples can represent a risk for public health. Therefore, an efficient control and effective measures were needed along the production chain to ensure the food safety.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global Impact of COVID-19 Pandemic on Gastrointestinal Infections: A Scoping Review.","authors":"Afroditi Lazarakou, Lapo Mughini-Gras, Roan Pijnacker","doi":"10.1089/fpd.2024.0047","DOIUrl":"10.1089/fpd.2024.0047","url":null,"abstract":"<p><p>During the COVID-19 pandemic, nonpharmaceutical public health interventions (NPIs) were implemented worldwide to control the spread of severe acute respiratory syndrome coronavirus 2. However, the incidence of other pathogens, including gastrointestinal (GI) pathogens, was also affected. Here, we reviewed studies assessing the impact of NPIs during the COVID-19 pandemic on the incidence of GI infections, particularly foodborne infections. A systems literature search was conducted in May 2023, using Living Evidence on COVID-19 (COAP) and Scopus. Articles were identified and selected through a screening process with inclusion and exclusion criteria based on the Preferred Reporting Items for Systematic Reviews and Meta-Analysis statement. Data were extracted from each full-text article included in the review. Parameters included were GI viruses, GI bacteria, NPIs against the COVID-19 pandemic, and the associated impact of NPIs on GI pathogens. A total of 42 articles were included in the review, representing 18 countries. Overall, a larger reduction was observed for viral GI infections compared with bacterial GI infections during the COVID-19 pandemic, particularly for norovirus. For bacterial GI infections, <i>Campylobacter</i> and nontyphoidal <i>Salmonella</i> were the most frequently detected pathogens in the majority of the studies, with the largest reduction observed for <i>Shigella</i> and Shiga toxin-producing <i>Escherichia coli</i> infections. The sharp decrease in GI viral infections in most of the included countries is suggested to be related to the disruption of person-to-person transmission due to several implemented interventions (e.g., social distancing and hand hygiene). GI bacterial pathogens, more commonly transmitted via the foodborne route, were least impacted, and their reduction is associated with closure of food-providing settings and travel restrictions. However, the observed changes appear to be multifactorial; alterations in health-care-seeking behaviors and in routinary diagnostic testing have undeniably played a significant role, affecting national surveillance systems. Therefore, although NPIs likely had a substantial impact on the burden of GI infectious diseases, the extent of the true change cannot be fully assessed.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Antibiotic Susceptibility of Nontyphoidal <i>Salmonella</i> from Food-Animal Products in Bejaia, Algeria.","authors":"Zahra Bellil, Sylvain Meyer, Valentin Tilloy, Assia Mairi, Christophe De Champs, Olivier Barraud, Abdelaziz Touati","doi":"10.1089/fpd.2024.0036","DOIUrl":"https://doi.org/10.1089/fpd.2024.0036","url":null,"abstract":"<p><p>Nontyphoidal <i>Salmonella</i> presents a significant threat to animal and human health as a food-borne infectious agent. This study focused on the characterization of <i>Salmonella</i> isolates obtained from fresh animal products consumed in Bejaia, Algeria. In total, 495 beef products, 600 chicken products, and 355 dairy products were collected from retail outlets and slaughterhouses in the region. Out of the 1450 samples collected, a <i>Salmonella</i> positivity rate of 3.5% (51/1450) was observed. Traditional Algerian sausages exhibited the highest prevalence of <i>Salmonella</i> contamination (14.1%, 36/256). Fifteen different serotypes were identified, with <i>S.</i> Kentucky (<i>n</i> = 12), <i>S.</i> Anatum (<i>n</i> = 11), and <i>S.</i> Bredeney (<i>n</i> = 9) being the most prevalent. Antimicrobial susceptibility testing showed resistance to diverse antibiotics, particularly against tetracycline, ampicillin, nalidixic acid, and sulphonamides. Whole-genome sequencing conducted on 21 isolates enabled the comparison of phylogenetic links between isolates. We notably identified clones circulating across the region in different locations and food types, suggesting contamination at the early stages of the food chain (in the herd or slaughterhouses) that disseminated to numerous butcher shops in various cities. We also identified acquired antibiotic resistance genes and point mutations in the quinolone resistance-determining region genes, contributing to the observed resistance patterns. This study describes the genomic and phylogenetic characteristics of several <i>Salmonella</i> clones found in diverse food samples in Algeria. It suggests potential transmission dynamics that could better understand <i>Salmonella</i>'s contamination routes.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infection Status, Etiological Analysis of <i>Aeromonas</i> Spp. in Foodborne Diarrhea Patients from 2019 to 2023 in Wenzhou.","authors":"Huihuang Lou, Yi Li, Yuejin Wu, Yuqin Hu, Leyi Zhang","doi":"10.1089/fpd.2024.0030","DOIUrl":"https://doi.org/10.1089/fpd.2024.0030","url":null,"abstract":"<p><p>The infection status and etiological analysis of <i>Aeromonas</i> spp. from foodborne diarrhea patients in Wenzhou were carried out to provide the etiological basis for healthy diet and clinical treatment. <i>Aeromonas</i> isolates (<i>n</i> = 41) collected from foodborne diarrhea patients were identified using the automatic bacteriologic analyzer and mass spectrometer. Species identification, multilocus sequence typing, prediction of virulence genes, and antimicrobial resistance genes were analyzed by the data of whole genome sequencing. The antibiotic resistance of these isolates was determined using miniaturization of the broth dilution susceptibility test. A total of 1829 stool samples of diarrhea patients were collected, and the detection rate of <i>Aeromonas</i> spp. was 2.24% (41/1829). Moreover, <i>Aeromonas</i> spp. are more easily detected in warmer months (from June to August), which were identified as follows: <i>A. veronii</i> (53.66%, 22/41), <i>A. caviae</i> (21.95%, 9/41), <i>A. hydrophila</i> (9.76%, 4/41), <i>A. dhakensis</i> (4.88%, 2/41), <i>A. rivipollensis</i> (4.88%, 2/41), <i>A. enteropelogenes</i> (2.44%, 1/41), and <i>A. media</i> (2.44%, 1/41). All strains can be divided into 38 sequence types, 31 of which were novel, suggesting that <i>Aeromonas</i> spp. had high genetic diversity, multiple clones, and various sources in diarrhea patients. High number of genetic diversity and resistance were found in the <i>Aeromonas</i> isolates. In addition, the category distribution of the virulence genes was significantly different among the seven species of <i>Aeromonas</i>. <i>Aeromonas</i> spp. had different degrees of resistance to antibiotics, and tetracycline was the most serious, with a resistance rate of 27%. What's more, for some antimicrobial classes <i>in silico</i> antimicrobial resistance gene detection was highly correlated with phenotypic antimicrobial resistance patterns with an overall sensitivity of 93.3% and a specificity of 66.7%. The findings from this research highlighted the importance for development of prevention and control strategies to reduce the risk of foodborne diarrhea caused by <i>Aeromonas</i> spp.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142675596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lateral Ventricle Neurocysticercosis: A Systematic Review of Case Reports and Case Series.","authors":"Zoran Milenković, Aleksandra Ignjatović, Marko Stalević, Marina Ranđelović, Goran Koraćević, Snežana Mladenović, Suzana Otašević","doi":"10.1089/fpd.2024.0075","DOIUrl":"https://doi.org/10.1089/fpd.2024.0075","url":null,"abstract":"<p><p><b><i>Purpose:</i></b> We performed a literature review focusing on case reports and case series studies, aiming to better define the clinical presentation of isolated lateral intraventricular neurocysticercosis (LVNCC) and to discuss the current knowledge of its characteristics, patient demographics, clinical manifestations, treatment, and prognosis, based on the collected data. <b><i>Methods:</i></b> Data for this study were gathered by conducting searches on the Medline database and Google Scholar using various combinations of the following terms \"intraventricular neurocysticercosis (IVNCC),\" \"brain ventricle cyst,\" \"cysticercosis of lateral brain ventricles,\" \"cysticercus cyst in brain ventricles,\" and \"intraventricular cystic brain lesion.\" Articles published in English between January 1980 and March 2023 that reported cases of LVNCC were selected for analysis. <b><i>Results:</i></b> This study included 48 patients (mean age 33.1 ± 14.1, range 6-70 years) diagnosed with LVNCC. Most patients were from India. The predominant clinical manifestation was headache (87.8%), followed by nausea/vomiting (51.2%), altered sensorium (51.2%), and focal neurological deficits (29.3%). In most cases, symptoms lasted from 10 d to 20 years (67.6%). The mean age at symptom onset was higher than in those with cysts in the third and fourth ventricles (<i>p</i> = 0.010058), and a greater proportion of vesicular cysts was observed (58.3%). Hydrocephalus was common (81.3%), with a significant percentage showing unilateral ventricular enlargement (38.5%). Surgical excision of the parasite (predominantly endoscopic) was the prevailing type of treatment (72.9%). Postoperatively, anti-helminthics were administered in 37.5% of cases. Most patients (80.5%) had favorable clinical outcomes or improved clinical status; six patients died, while the clinical outcomes of seven individuals were not specified in reports. <b><i>Conclusion:</i></b> LVNCC is a rare form of NCC, typically characterized by symptoms lasting >7 d. Invasion of the ventricle by cysticerci occurs mainly in middle-aged individuals. Endoscopy is the preferred treatment option, although the prognosis is influenced by various factors. Mortality is high in untreated patients.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142647312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Rapid Colorimetric and Fluorescent Assay of <i>Aspergillus Fumigatus</i> in Food by Loop-Mediated Isothermal Amplification.","authors":"Ji Lv, Yun Tian, Wenping Liu, Haitian Fang, Lei Xiong, Lu Cao, Junping Zheng, Hongtao Liu","doi":"10.1089/fpd.2024.0092","DOIUrl":"https://doi.org/10.1089/fpd.2024.0092","url":null,"abstract":"<p><p><i>Aspergillus fumigatus</i> is a foodborne mycete that can induce recurrent pneumonia, but the current detection methods have insufficient sensitivity and rapidity. Here, we aim to develop an efficient and sensitive loop-mediated isothermal amplification (LAMP) primer set for <i>A. fumigatus</i> detection. First, we designed a novel set of LAMP primers by targeting the <i>Beta-tubulin (β-tub)</i> gene. The LAMP reaction system was optimized by screening reaction temperature and betaine concentration. And then, the specificity of the proposed primers was verified by using 10 interferent microorganism species. The sensitivity of the designed method was compared with that of polymerase chain reaction (PCR) on pure cultures and complex matrix. The accuracy and response time of the method were examined by simulated samples. Our proposed primer set could accurately detect <i>A. fumigatus</i> from different food matrices with no response to other microorganisms. More intriguingly, this method possessed a low limit of detection (2 copies/reaction, 10-fold less than PCR), a short measuring time (<30 min), and a naked-eye readability. A real sample test demonstrates the good recovery rate and accuracy in apple, corn, milk, and other food matrix. Our proposed <i>β-tub</i> primer set provides great potential for rapid assessment of <i>A. fumigatus</i> contamination in food by integrating portable equipment and microscale reaction system.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142616955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Antibacterial Effect of Lactic Acid Bacteria and Baicalin Against <i>Staphylococcus aureus In Vitro</i> and <i>In Vivo</i>.","authors":"Yan-Ni Mao, Yan-Jun Ma, Gui-Qin Wang","doi":"10.1089/fpd.2024.0086","DOIUrl":"https://doi.org/10.1089/fpd.2024.0086","url":null,"abstract":"<p><p>Pathogenic bacteria such as <i>Staphylococcus aureus</i> (<i>S. aureus</i>) are the principal cause of cow mastitis, which primarily impacts milk yield and results in significant financial losses for the animal husbandry industry. Lactic acid bacteria-cell-free supernatant (LAB-CFS) and baicalin (BAI) both have a number of biological effects, including decreasing inflammation. The combined use of LAB-CFS and BAI does not appear to have been used to protective against mastitis, however, and the underlying mechanisms are yet unknown. In this study, <i>in vitro</i> activity of LAB-CFS and BAI alone and in combination was determined (checkerboard experiments, time-kill curves, and flow cytometry to investigate membrane permeability) and examined the protective effects of LAB-CFS and BAI on <i>S. aureus</i>-induced mastitis in mice and the impact of NF-κB signaling pathways on the emergence of mastitis. We discovered that when LAB-CFS and BAI were used together, <i>S. aureus</i> was more effectively treated than when LAB-CFS and BAI were used separately. Flow cytometry demonstrated that LAB-CFS and BAI work together to kill bacteria. <i>In vivo</i>, the usage of LAB-CFS and BAI decreased the activity of myeloperoxidase, as well as IL-6, IL-1β, and TNF-α secretion and the levels of TLR2 and p65 (NF-κB) expression. These findings suggested that LAB-CFS and BAI had a preventive effect against mastitis brought on by <i>S. aureus</i>. Therefore, the NF-κB signaling pathway is thought to be the likely mechanism through which LAB-CFS and BAI reduced <i>S. aureus</i>-induced inflammation in the mammary of cows. For the treatment of cow mastitis, LAB-CFS and BAI are likely to replace antibiotics.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142616986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Possibility of Vertical Transmission of <i>Sarcocystis</i> Spp. in Sika Deer in Japan.","authors":"Akiko Yamazaki, Yoshitaka Yamaguchi, Tatsuya Hiroshima, Yui Urushibara, Yukiko Shirafuji, Shinya Fukumoto, Yoichi Kamata","doi":"10.1089/fpd.2024.0090","DOIUrl":"https://doi.org/10.1089/fpd.2024.0090","url":null,"abstract":"<p><p>In recent years, the wild deer population in Japan has grown exponentially, causing severe feeding damage to the agricultural and forestry industries. Therefore, the game meat industry is being promoted for effective utilization of hunted animals. Wild animals are not hygienically controlled and can serve as reservoirs for pathogenic microorganisms. However, epidemiological information on wild animals in Japan remains insufficient. Recently, food poisoning-like cases have occurred because of raw venison infection with <i>Sarcocystis</i> spp. As the prevalence of <i>Sarcocystis</i> spp. in sika deer is very high in Japan and even fawns are infected, this study attempted to verify the vertical infection of <i>Sarcocystis</i> spp. in sika deer in Japan. Genetic detection of <i>Sarcocystis</i> 18S ribosomal RNA in fetal and maternal tissues from early to late gestation in sika deer revealed <i>Sarcocystis</i> Types 1-5 and <i>Sarcocystis fayeri</i> in the mother and fetus. Types 1, 2, 4, and 5 were detected in the maternal tissues of Ezo sika deer (<i>Cervus nippon yesoensis</i>) in Hokkaido, whereas Types 1 and 2 and <i>S. fayeri</i> were detected in fetuses. Types 1-5 were detected in Honshu sika deer (<i>Cervus nippon centrali</i>s) in Mie Prefecture but not in the fetuses. Types 1, 2, and 4 were detected in the udder and milk samples. This indicates that <i>Sarcocystis</i> Types 1 and 2 and <i>S. fayeri</i> have the ability to pass through the placenta of sika deer and invade fetal tissues and Types 1, 2, and 4 may be transmitted orally via milk. These findings suggest that there is transplacental and transmammary transmission of <i>Sarcocystis</i> spp. in sika deer.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142616962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virulence Profiles of <i>Salmonella enterica</i> Isolated from Three Food Matrices Collected from Retail Markets.","authors":"Sandy V Alarcón Navas, Eliana M Pereira Cardeño, María F Martínez, Nicolás F Ortiz Suárez, Alexander David Castro, Ruth A Martínez-Vega, Marcela Navarro Rosado, Clara I González, Giovanna Rincón Cruz","doi":"10.1089/fpd.2024.0104","DOIUrl":"https://doi.org/10.1089/fpd.2024.0104","url":null,"abstract":"<p><p><i>Salmonella enterica</i> is one of the most common foodborne pathogens associated with the consumption of contaminated porcine, dairy, and avian products. Nontyphoidal <i>Salmonella</i> is a major cause of bacterial diarrhea, responsible for ∼150 million cases and 60,000 deaths annually. The main goal of this study was to determine the prevalence of <i>Salmonella</i> spp. and to establish the virulence profile (VP) from genes (<i>avr</i>A, <i>inv</i>E, <i>ssa</i>D, <i>sse</i>F, <i>ssa</i>Q, <i>ttr</i>C) and plasmid genes (<i>pef</i>A, <i>spv</i>B, <i>spv</i>C) in isolates obtained from cheese, chicken, and pork sold in food markets in Barrancabermeja, Colombia. A survey was conducted on 100 samples each matrix. The detection of <i>Salmonella</i> spp. followed the ISO 6579:2017 standards modified, and isolates were confirmed using the <i>inv</i>A gene. In addition, single polymerase chain reaction assays were developed to detect the nine virulence genes. <i>Salmonella</i> spp. was found in 62%, 32%, and 14% of pork, chicken, and cheese samples, respectively. A total of 277 isolates were biochemically, serologically, and molecularly compatible with <i>Salmonella</i> spp. The most representative serogroups were C and B. Forty-seven combinations of virulence gene were detected; 53.5% of the pork isolates, 46.2% of the cheese isolates, and 39% of the chicken isolates were distributed among VP1, VP2, and VP3 suggesting a higher pathogenic potential. In addition, seven isolates harbored plasmid-encoded virulence genes (<i>spv</i>B and <i>spv</i>C), which are associated with increased invasiveness. The results revealed a higher prevalence of <i>Salmonella</i> spp. in pork and chicken compared with other studies conducted in Colombia. The serogroups identified include serovars that more frequently affect humans <i>Salmonella</i> Enteriditis, <i>Salmonella</i> Newport, and <i>Salmonella</i> Typhimurium. The isolations have the majority of the virulence genes studied. These findings highlight the need to improve control measures and educate food handlers to minimize the presence of <i>Salmonella</i> spp. and its potential transmission.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142616990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Flagella on <i>Salmonella</i> Enteritidis Sedimentation, Biofilm Formation, Disinfectant Resistance, and Interspecies Interactions.","authors":"Huixue Hu, Jingguo Xu, Jingyu Chen, Chao Tang, Tianhao Zhou, Jun Wang, Zhuangli Kang","doi":"10.1089/fpd.2024.0088","DOIUrl":"https://doi.org/10.1089/fpd.2024.0088","url":null,"abstract":"<p><p>Flagella are essential for bacterial motility and biofilm formation by aiding bacterial attachment to surfaces. However, the impact of flagella on bacterial behavior, particularly biofilm formation, remains unclear. This study constructed two flagellar mutation strains of <i>Salmonella</i> Enteritidis (SE), namely, SE-Δ<i>flhD</i> and SE-Δ<i>flgE</i>, and confirmed the loss of flagellar structures and motility in these strains. The mutant strains exhibited growth comparable with the wild-type (WT) strain but had higher sedimentation rates. Biofilm biomass did not differ significantly between the WT and mutant strains, except for SE-Δ<i>flgE</i> at 3 d. SE-Δ<i>flgE</i> showed increased susceptibility to sodium hypochlorite compared to the WT. The co-sedimentation rate of flagella-deficient strains was lower than the WT, and the biomass of dual-species biofilm formed by <i>Bacillus paramycoides</i> B5 with SE-Δ<i>flhD</i> or SE-Δ<i>flgE</i> was significantly lower than with the WT. These findings emphasize the significance of SE flagella in biofilm formation and interspecies interactions, offering insights into targeted biofilm prevention and control measures.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142603916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}