Foodborne pathogens and disease最新文献

{"title":"Typhoid Fever in the Republic of Korea, 2001-2024: Long-Term Decline, Residual Annual Periodicity, and Southeastern Coastal Spatial Clustering in 24 Years of Nationwide Surveillance.","authors":"Geonsang Lee, Seongdae Kim","doi":"10.1177/15353141261465221","DOIUrl":"https://doi.org/10.1177/15353141261465221","url":null,"abstract":"<p><p>Typhoid fever (<i>Salmonella</i> Typhi) has declined dramatically in Korea over three decades, but residual seasonality, demographic patterns and provincial spatial concentration have not been systematically reanalysed. Using all 3535 typhoid cases reported to the Korea Disease Control and Prevention Agency between 2001 and 2024 (3051 domestic and 484 imported [13.7%]), we quantified the long-term annual trend with the Hamed-Rao modified Mann-Kendall test (τ = -0.667, <i>p</i> = 0.0011) and a negative-binomial generalized linear model (GLM) yielding a Sen slope of -7.3%/year (95% CI -9.9 to -4.4); a parametric-bootstrap analysis of the runner-up 1-breakpoint GLM identified 2018 as the most likely structural change point (95% CI [2009, 2022]). A continuous Morlet wavelet transform of the weekly series revealed annual periodicity that exceeded a 1000-simulation AR(1) red-noise null by a factor of 2.60. Direct age standardization and age-band-specific Mann-Kendall tests showed statistically significant declines in every age stratum. Provincial spatial analysis (16 sido) gave a global Moran's I of 0.403 (<i>p</i> = 0.0083, 9999 permutations) under a Queen + k-nearest-neighbor-2 baseline scheme, with the southeastern coastal cluster (Gyeongnam-Busan-Ulsan-Gyeongbuk axis) preserved across 12 alternative weighting schemes after empirical Bayes shrinkage of sigungu-level rates and across two equal-length time strata; one province (Ulsan) survived Benjamini-Hochberg FDR adjustment of the 16 LISA <i>p</i>-values. Korean typhoid has continued its long-term decline; residual incidence concentrates in the southeastern coastal corridor, and imported cases-including ciprofloxacin-resistant H58 strains from South Asia-now form a substantial fraction of notifications, motivating pre-travel counseling and post-travel vigilance.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"15353141261465221"},"PeriodicalIF":2.3,"publicationDate":"2026-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148351246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meta-Analysis on Foodborne Pathogens in Animal-Derived Foodstuffs in Maghreb Countries.","authors":"Amir Nahal, Sofiane Tamendjari","doi":"10.1177/15353141261464403","DOIUrl":"https://doi.org/10.1177/15353141261464403","url":null,"abstract":"<p><p>Animal-derived foodstuffs are essential for human nutrition but may harbor pathogens responsible for foodborne diseases. In Maghreb countries (Algeria, Libya, Morocco, and Tunisia), data on foodborne pathogen hazards are often limited and fragmented. To address this gap, the present systematic review and meta-analysis aimed to estimate the overall prevalence of foodborne pathogens in animal-derived foods in the Maghreb region from 2004 to 2025. A systematic literature search was conducted in two electronic databases (PubMed and Scopus) following PRISMA guidelines. A total of 80 studies met the inclusion criteria. Data were analyzed using the meta package in R software, applying a random-effects model. The pooled prevalence of pathogens in foods of animal origin was 26.6% (95% CI: 20.0-33.8), with high heterogeneity (<i>I²</i> = 98.9%). The highest contamination was observed in raw poultry meat (36.1%). Eleven foodborne bacterial species were reported. <i>Salmonella</i> spp. was the most frequently reported (<i>n</i> = 18), with a pooled prevalence of 14.2%, followed by <i>Staphylococcus aureus</i> (28.3%, <i>n</i> = 16) and <i>Escherichia coli</i> (36%, <i>n</i> = 16). Most studies were conducted in Algeria and Morocco, with country-level prevalence ranging from 22.1% to 38.1%. This review reveals a substantial burden of foodborne pathogens in the Maghreb region. Enhanced surveillance and targeted control measures are essential to improve food safety and protect public health.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"15353141261464403"},"PeriodicalIF":2.3,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148344393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Digesting the Data: Overview of Foodborne Outbreaks in Canada Submitted to Outbreak Summaries, 2008-2023.","authors":"Elizabeth Schutt, Emily Thompson, Leslie Cheng, Anne-Marie Lowe, Melissa Phypers","doi":"10.1177/15353141261462750","DOIUrl":"10.1177/15353141261462750","url":null,"abstract":"<p><p>Outbreak Summaries is a Canadian enteric, food, and waterborne outbreak surveillance system designed to support the collection and analysis of Canadian outbreak data. This analysis summarizes the foodborne outbreak investigations submitted between 2008 and 2023. Finalized reports of foodborne outbreak investigations submitted by all participating jurisdictions were extracted and characterized by pathogen, outcomes, food vehicles, and pathogen-food vehicle pairs. Annual trends in submissions and jurisdictional scope were explored. A total of 452 foodborne outbreaks were submitted by 11 participating provinces and territories alongside the Public Health Agency of Canada included in the analysis. A total of 14,650 illnesses, 1268 hospitalizations, and 68 deaths were reported. Almost 70% of all submitted outbreaks were caused by bacteria, and viruses accounted for 20%. <i>Salmonella</i> was the most common cause of outbreaks (184/434, 42% of outbreaks with a known pathogen) and outbreak-associated illnesses (7003/14,418, 48%), followed by Norovirus outbreaks (72/434, 16%) and outbreak-associated illnesses (2599/14,418, 18%). The highest absolute number of hospitalizations was attributed to <i>Salmonella</i> (<i>n</i> = 796), followed by <i>Escherichia coli</i> (<i>n</i> = 249), and <i>Listeria monocytogenes</i> (<i>n</i> = 97). <i>L. monocytogenes</i> had the highest hospitalization rate (94%, <i>n</i> = 97), and case fatality rate (17%, <i>n</i> = 35). A confirmed or suspected food vehicle was identified in 230 outbreaks (51%), accounting for 10,587 outbreak-associated cases (72%). The most frequently identified food vehicles were raw vegetables (<i>n</i> = 36, 15.7%), chicken (<i>n</i> = 29, 12.6%), and fresh shellfish (<i>n</i> = 17, 7.4%). The pathogen-food pairs associated with the most outbreaks were <i>Salmonella</i> in chicken (26/221) and whole eggs (15/221) and <i>E. coli</i> in raw vegetables (13/221). Analysis of foodborne outbreaks in the context of pathogens and food vehicles supports the investigative work of public health authorities in Canada. The expansion of program participants and more than 15 years of surveillance data provide a more comprehensive understanding of the foodborne outbreak landscape in Canada.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"15353141261462750"},"PeriodicalIF":2.3,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148276723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Redox Modulation and Performance Outcomes in Broilers Supplemented with a Phytogenic Anti-Mycotoxin Agent.","authors":"Vasileios G Papatsiros, Zoi Prentza, Christos Eliopoulos, Georgios I Papakonstantinou, Nikoleta Polychronidou, Dimitrios Arapoglou, Eleftherios Meletis, Óscar Castro, Nikolaos Voulgarakis, Insaf Riahi, Polychronis Kostoulas, Konstantinos Koutoulis, Dimitra Psalla","doi":"10.1177/15353141261461963","DOIUrl":"10.1177/15353141261461963","url":null,"abstract":"<p><p>This study evaluated the effects of a phytogenic-based liquid anti-mycotoxin agent (grape and olive extracts) on the redox status, health, and performance of broilers. The experimental broilers were divided into two groups: (a) group C (control group) with 22,950 broilers and (b) group T (treatment group) with 23,256 broilers, including administration of 500 mL/ton of the tested agent in drinking water on the 9th-11th, and on the 23rd-25th and 31st-33rd day. A liquid chromatography tandem mass spectrometry with a quadrupole time of flight analyzer was performed with fungal metabolites on liver samples. Thiobarbituric acid reactive substances (TBARSs), protein carbonyls (CARBs), total antioxidant capacity (TAC), total protein, and liver enzymes (alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase) were determined in plasma. The relative abundance of the tenuazonic acid (TeA) was calculated for both groups, showing that the exposure of TeA was 83% lower in group T. TBARS and CARBs, as well as the levels of liver enzymes and total proteins, were significantly reduced, while TAC was increased in group T. No differences in mortality rate and a potential improvement in the growth performance of group T were noticed. In conclusion, administering a phytogenic-based liquid anti-mycotoxin agent to broiler positively affects redox status and mycotoxin management.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"15353141261461963"},"PeriodicalIF":2.3,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148276716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Virulence Factors, Biofilm Production, and Antimicrobial Resistance in Mastitis-Associated <i>Staphylococcus aureus</i> Strains by Phenotypic and Genotypic Methods.","authors":"Richa Sarkar, Ravi Kant Agrawal, Kajal Bankoti, Jaideep Singh, Keshav Kumar, Mrinalini Saini, Debajit Roy, Gaurav Kumar Sharma, Vidya Singh, Mudit Chandra, Bhoj Raj Singh","doi":"10.1177/15353141261460684","DOIUrl":"https://doi.org/10.1177/15353141261460684","url":null,"abstract":"<p><p><i>Staphylococcus aureus</i>, a common cause of bovine mastitis, relies on several virulence factors, with biofilm formation being a key contributor to its pathogenicity. The present study investigated the occurrence of <i>S. aureus</i> as etiological agents in bovine mastitis with a focus on the existence of various virulence factors and antibiotic resistance status. Among 120 milk samples collected from West Bengal and Uttar Pradesh, 36 (30%) <i>S. aureus</i> strains were confirmed by conventional methods and PCR. Phenotypic analysis revealed hemolysin (55.55%) and coagulase production (36.11%), while molecular analysis revealed the presence of leukotoxin (<i>luks</i>F, 19.44%), hemolysin (<i>hlb</i>, 58.33%), coagulase (<i>coa</i>, 63.88%), and toxic shock syndrome toxin (<i>tsst</i>-1, 30.55%) genes. Biofilm production ability was detected in 97.22% (crystal violet assay) and 86.11% (Congo red agar assay) strains. Biofilm-associated genes, namely, <i>icaA</i> (80.55%, 29/36), <i>icaB</i> (75%, 27/36), <i>icaC</i> (69.44%, 25/36), <i>icaD</i> (86.11%, 31/36), and MSCRAMMs genes, namely, <i>clfA</i> (58.33%, 21/36), <i>clfB</i> (75%, 27/36), <i>fnbA</i> (75%, 27/36), <i>fnbB</i> (55.55%, 20/36), <i>bap</i> (38.88%, 14/36), <i>bbp</i> (83.33%, 30/36), <i>ebps</i> (69.44%, 25/36), <i>eno</i> (66.66%, 24/36), <i>fib</i> (41.66%, 15/36), and <i>cna</i> (8.33%, 3/36), were also detected. Antimicrobial resistance was observed in 88.88% isolates, with 72.22% exhibiting multidrug resistance (MDR). Among the isolates, 83.33% were methicillin-resistant <i>S. aureus</i> (MRSA), and <i>mecA</i>, <i>femA</i>, and <i>femB</i> genes were present either singly or in combination in 76.66% of the isolates. Efflux pump protein genes, namely, <i>norA</i>, <i>norB</i>, <i>norC</i>, <i>mdeA</i>, <i>mepA</i>, and <i>sepA</i>, were detected either singly or in combination in <i>S. aureus</i> isolates. 61.53% of MDR-MRSA isolates harbored all six efflux pump genes. According to this study, <i>S. aureus</i> of mastitis origin harbors various virulence, antibiotic resistance, biofilm-forming, and efflux pump genes. Bovine mastitis-derived MDR <i>S. aureus</i> isolates can pose a significant public health risk and need urgent attention to formulate strategies for their control and preventing transfer to the human food chain.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"15353141261460684"},"PeriodicalIF":1.9,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148258020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gastric Infection by <i>Cronobacter sakazakii</i>: An <i>In Vitro</i> Insight.","authors":"Babak Pakbin, Susanne U Mertens-Talcott","doi":"10.1177/15353141261461975","DOIUrl":"https://doi.org/10.1177/15353141261461975","url":null,"abstract":"<p><p><i>Cronobacter sakazakii</i>, an opportunistic foodborne pathogen associated with powdered infant formula, causes severe neonatal infections, yet its interaction with gastric epithelial cells remains poorly characterized. Here, we conducted a preliminary <i>in vitro</i> study to evaluate early host-pathogen responses of <i>C. sakazakii</i> in AGS gastric epithelial cells. AGS cells were exposed to <i>C. sakazakii</i> ATCC 29544 at a multiplicity of infection of 100 for 2 h, and cytotoxicity and transcriptional responses were assessed by viability assay, gentamicin protection assay, and reverse transcription-quantitative polymerase chain reaction. Infection significantly reduced AGS cell viability and increased expression of bacterial virulence-associated genes (<i>ompA</i> and <i>hfq</i>) and host inflammatory genes (<i>IL-8</i> and <i>NF-ƙB</i>) compared with mock-infected controls (<i>p</i> < 0.001). Gentamicin-protected recovery was consistent with bacterial entry into AGS cells, although confirmatory imaging-based analyses were not performed. These findings indicate that <i>C. sakazakii</i> (ATCC 29544) can elicit early cytotoxic and pro-inflammatory responses in AGS gastric epithelial cells under <i>in vitro</i> conditions, supporting the need for confirmatory studies in more physiologically relevant gastric study models.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"15353141261461975"},"PeriodicalIF":1.9,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148257975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic Features of <i>Campylobacter jejuni</i> and <i>Campylobacter coli</i> Poultry Isolates from the Western Part of Romania.","authors":"Iulia Adelina Bundurus, Lavinia Ștef, Ozan Gundogdu, Ioan Pet, Nicolae Corcionivoschi, Igori Balta","doi":"10.1177/15353141261460746","DOIUrl":"https://doi.org/10.1177/15353141261460746","url":null,"abstract":"<p><p><i>Campylobacter jejuni</i> and <i>Campylobacter coli</i> are leading causes of foodborne gastroenteritis worldwide, with poultry representing the primary reservoir for human infection. Understanding species-specific differences in virulence-associated features, antimicrobial resistance genes, and population structure is essential for assessing public health risks and guiding surveillance strategies. A total of 26 <i>Campylobacter</i> isolates (22 <i>C. coli</i> and 4 <i>C. jejuni</i>) were recovered in 2023 from poultry caeca collected from farms in north-western, central-western, and south-western Romania. Species identification and detection of the <i>hcp</i> gene, a marker of the type VI secretion system (T6SS), were performed using multiplex PCR. Multilocus sequence typing (MLST) based on seven housekeeping genes was performed to evaluate genetic diversity and population structure. The <i>hcp</i> gene was detected exclusively in <i>C. jejuni</i> isolates, indicating that it was detected only in <i>C. jejuni</i> within this collection of the primary T6SS component. Both species shared a broad set of core virulence genes related to motility, colonization, quorum sensing, adhesion, and surface polysaccharide biosynthesis, while notable differences were observed in accessory virulence gene profiles. Genes associated with antibiotic resistance, including the tetracycline resistance gene <i>tet</i>(O) and multiple efflux-related determinants, were frequently detected in both species. MLST analysis revealed high genetic diversity, with most isolates not assignable to previously defined sequence types, consistent with an open pan-genome and substantial genomic plasticity. These findings feature clear differences between <i>C. jejuni</i> and <i>C. coli</i> in the distribution of virulence markers, while validating the widespread presence of antimicrobial resistance genes in poultry-associated isolates. This study highlights the adaptive potential of <i>Campylobacter</i> and underscores the need to advance molecular surveillance to monitor virulence and antimicrobial resistance risks relevant to animal and human health.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"15353141261460746"},"PeriodicalIF":1.9,"publicationDate":"2026-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148249932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Resistance and Genomic Insights into <i>Enterococcus faecalis</i> Isolates from Broilers and Their Handlers in Punjab, India.","authors":"Neha Parmar, Randhir Singh, Anuj Tyagi, Simranpreet Kaur, Hina Malik, Rabinder Singh Aulakh, Anil Kumar Arora, Jatinder Paul Singh Gill","doi":"10.1089/fpd.2024.0151","DOIUrl":"10.1089/fpd.2024.0151","url":null,"abstract":"<p><p><i>Enterococcus faecalis</i>, a common constituent of human and animal gut microbiota, has emerged as a significant pathogen due to its antimicrobial resistance (AMR) capabilities. This study investigates AMR profiles, virulence factors, and genetic diversity of <i>E. faecalis</i> isolates from broiler farms in Ludhiana, Punjab, India, to explore transmission dynamics between poultry and handlers. A total of 240 samples were collected from 20 farms, comprising 200 broiler droppings, 34 hand swabs from poultry handlers, and 6 human stool samples. Selective media and standard microbiological techniques were employed to isolate and identify <i>E. faecalis</i>. Whole genome sequencing (WGS) was performed on representative isolates to uncover antimicrobial resistance genes (ARGs) and virulence factors. <i>E. faecalis</i> was isolated from 47% of broiler droppings and 26.47% of handler hand swabs, whereas no isolates were recovered from stool samples. High resistance was observed for erythromycin (96.11%), tetracycline (78.64%), ciprofloxacin (76.69%), streptomycin (76.69%), and linezolid (65.04%), with 83.49% of isolates exhibiting multidrug resistance (MDR). Vancomycin resistance genes (<i>vanC1</i>-34.95% and <i>vanC2/C3</i>-9.70%) were also detected. WGS analysis of four isolates identified ARGs such as <i>dfrE</i>, <i>efrA</i>, <i>vanT</i> gene in <i>vanG</i> cluster, <i>vanY</i> gene in <i>vanB</i> cluster, <i>ermB</i>, <i>tet45</i>, <i>tetM</i>, <i>tetA</i>, <i>aac(6')-Ie-aph(2″)-Ia</i>, <i>ant(4')-Ib</i>, <i>aph(3')-Ia</i>, <i>sul1</i>, <i>sul3</i>, <i>mphA</i>, <i>qnrS1</i>, and <i>catA8</i>, as well as virulence factors associated with biofilm formation, adherence, capsule formation, and protease production. Sequence typing identified was ST-1866, ST-7317, and ST-403, with ST-7317 common between broiler droppings and handler swab isolates, indicating potential transmission. While these findings highlight poultry environments as reservoirs for MDR <i>E. faecalis</i>, the directionality of transmission (zoonotic vs. reverse zoonotic) remains unclear. This underscores the need for expanded surveillance and molecular studies to better understand transmission dynamics and mitigate risks to farm workers and public health.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"373-383"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143987345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold Limonene Vapor and UV Treatment Against <i>Salmonella</i> Enteritidis and <i>Staphylococcus aureus</i> in Quail Eggs: Potential Mode of Action.","authors":"Narumol Matan, Katthayawan Khunjan","doi":"10.1089/fpd.2025.0035","DOIUrl":"10.1089/fpd.2025.0035","url":null,"abstract":"<p><p>This study aimed to develop a novel, refrigeration-free preservation method to extend quail egg shelf life and enhance consumer safety. The antimicrobial efficacy of limonene vapor against <i>Salmonella</i> Enteritidis and <i>Staphylococcus aureus</i> was evaluated. Limonene nanoemulsions (0.04%, 0.08%, and 0.12%) were used to generate vapor for coating eggshells. The coating process was performed using an ultrasonic device with a controllable temperature chamber to maintain different temperatures: 4°C (cold), 25°C (medium), and 37°C (high temperature). The antimicrobial efficacy was enhanced by applying UV irradiation for 10 min. The mode of action was analyzed through bacterial morphology, biofilm formation, and cell leakage. Limonene vapor (0.08%) at 4°C with ultraviolet (UV) reduced <i>Salmonella</i> Enteritidis and <i>S. aureus</i> by 6-7 log₁₀ on eggshells, whereas at 25°C and 37°C, the reductions were only 3.5 log₁₀ and 2.8 log₁₀, respectively. <i>Salmonella</i> was not detected, and <i>S. aureus</i> remained within food safety standards when quail eggs were stored at 30 ± 2°C for 10 days, compared with the control, which showed bacterial growth within one day. Possible mechanisms of action suggested that cold-generated limonene formed a thin film on the eggshell surface. This film caused deformation and abnormal morphology in <i>Salmonella</i> Enteritidis cells and induced pore formation in <i>S. aureus</i> cell walls, enhancing bacterial cell injury. UV exposure further exacerbated cellular damage, resulting in a synergistic antimicrobial effect. However, this method did not cause significant membrane damage or lead to substantial leakage of intracellular materials in <i>S. aureus</i>. This method was cost-effective and could be adapted for on-farm application before distribution, ensuring safer eggs for consumers.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"384-390"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143980907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection and Antibiotic Susceptibility Profile of Bacterial Pathogens Isolated from Food Cutting Boards in Rio De Janeiro, Brazil.","authors":"Bianca de Souza Costa, Katarine Coutinho Silva, Carolaine Totelote Medeiros Pimenta Rodrigues, Ana Carolina Carvalho De Oliveira, Silvia Maria Dos Reis Lopes, Ivano de Filippis","doi":"10.1089/fpd.2024.0182","DOIUrl":"10.1089/fpd.2024.0182","url":null,"abstract":"<p><p>We investigated bacterial pathogens on restaurant cutting boards using phenotypic and molecular methods, and determined their antibiotic susceptibility. A total of 32 samples were collected from polyethylene cutting boards in the kitchens of 16 restaurants in Rio de Janeiro, Brazil. A total of 29 bacterial strains were isolated and identified, including <i>Salmonella</i> spp. (<i>n</i> = 11), <i>Escherichia coli</i> (<i>n</i> = 10), <i>Staphylococcus aureus</i> (<i>n</i> = 6), and <i>Listeria</i> spp. (<i>n</i> = 2). Of the 32 cutting boards, 9 (28.1%) harbored at least one bacterial species, 7 (21.9%) had two species, and 2 (6.2%) carried three species. Notably, the detection of two pathogens directly from raw samples using quantitative polymerase chain reaction with high-resolution melting (qPCR-HRM) was significant: <i>Listeria monocytogenes</i> was identified in two samples by phenotypic methods but in 18 samples by qPCR-HRM, while <i>E. coli</i> was found in 10 samples by phenotypic methods and in 29 samples by qPCR-HRM. Antibiotic resistance was another concern, especially for <i>L. monocytogenes</i> strains, which exhibited high resistance to all tested antibiotics. Additionally, multidrug-resistant strains were identified, including a <i>S. aureus</i> strain with strong potential to be methicillin-resistant. Our findings underscore the importance of molecular methods for detecting specific pathogens on food-processing surfaces, highlighting the need for improved monitoring and control measures in restaurant kitchens.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"355-361"},"PeriodicalIF":1.9,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143964314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}