Antimicrobial Resistance Profiles and Molecular Characteristics of Extended-Spectrum β-Lactamase-Producing Salmonella enterica Serovar Typhimurium Isolates from Food Animals During 2010-2021 in South Korea.

IF 1.9 2区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY

Foodborne pathogens and disease Pub Date : 2024-10-01 Epub Date: 2024-07-19 DOI:10.1089/fpd.2023.0128

Md Sekendar Ali, Seok-Hyeon Na, Bo-Youn Moon, Hee Young Kang, Hee-Seung Kang, Su-Jeong Kim, Tae-Sun Kim, Ye-Eun Heo, Yu-Jeong Hwang, Soon Seek Yoon, Suk-Kyung Lim

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引用次数: 0

Abstract

Extended-spectrum β-lactamase (ESBL)-producing Salmonella is emerging as a worldwide public health concern. In this study, we aimed to investigate the antimicrobial resistance profiles and molecular characteristics of ESBL-producing Salmonella enterica serovar Typhimurium (S. Typhimurium). We obtained a total of 995 S. Typhimurium isolates from the feces and carcasses of pigs (n = 678), chickens (n = 202), and cattle (n = 115) during 2010-2021 in Korea. We found that 35 S. Typhimurium isolates (3.5%) showed resistance to ceftiofur: pigs (51.4%, 18/35) and cattle (42.9%, 15/35). All of the ceftiofur-resistant S. Typhimurium isolates demonstrated multidrug resistance. Moreover, ceftiofur-resistant S. Typhimurium isolates displayed significantly higher rates of resistance to chloramphenicol and trimethoprim/sulfamethoxazole than ceftiofur-susceptible S. Typhimurium isolates (p < 0.05). The ceftiofur-resistant S. Typhimurium isolates produced four different CTX-M-type β-lactamase, comprising bla_CTX-M-55 in the majority (51.4%, 18/35), followed by bla_CTX-M-65 (28.6%, 10/35), bla_CTX-M-14 (17.1%, 6/35), and bla_CTX-M-1 (2.9%, 1/35). Among the 35 ceftiofur-resistant S. Typhimurium isolates, 16 bla_CTX-M-55-positive isolates and one bla_CTX-M-1-positive isolate were transferred to recipient Escherichia coli RG488 by conjugation. The predominantly found transposable units were bla_CTX-M-55-orf477 (45.7%, 16/35), followed by bla_CTX-M-65-IS903 (28.6%, 10/35) and bla_CTX-M-14-IS903 (17.1%, 6/35). Ceftiofur-resistant S. Typhimurium represented 19 types, with types P1-19 (22.9%, 8/35) and P12-34 (22.9%, 8/35) making up the majority and being found in most farms nationwide. Sequence types (STs) were different by animal species: ST19 (48.6%, 17/35) and ST34 (42.9%, 15/35) were mostly found STs in pigs and cattle, respectively. These findings showed that food animals, especially pigs and cattle, act as reservoirs of bla_CTX-M-harboring S. Typhimurium that can potentially be spread to humans.

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2010-2021 年间韩国食用动物肠道沙门氏菌中产广谱 β-乳酰胺酶沙门氏菌的抗菌谱和分子特征。

产广谱β-内酰胺酶（ESBL）沙门氏菌正在成为全球公共卫生关注的问题。在本研究中，我们旨在调查产ESBL鼠伤寒沙门氏菌（S. Typhimurium）的抗菌谱和分子特征。2010-2021 年间，我们从韩国猪（678 例）、鸡（202 例）和牛（115 例）的粪便和尸体中获得了共计 995 例秋伤寒沙门氏菌分离物。我们发现，35 个秋伤寒杆菌分离物（3.5%）对头孢噻呋有抗药性：猪（51.4%，18/35）和牛（42.9%，15/35）。所有耐头孢噻呋的伤寒杆菌分离物均表现出多药耐药性。此外，耐头孢噻呋的伤寒杆菌分离株对氯霉素和三甲双胍/磺胺甲噁唑的耐药率明显高于对头孢噻呋敏感的伤寒杆菌分离株（p < 0.05）。耐头孢噻呋菌分离物产生四种不同的 CTX-M 型 β-内酰胺酶，其中以 blaCTX-M-55 为主（51.4%，18/35），其次是 blaCTX-M-65（28.6%，10/35）、blaCTX-M-14（17.1%，6/35）和 blaCTX-M-1（2.9%，1/35）。在 35 个耐头孢噻呋菌分离株中，16 个 blaCTX-M-55 阳性分离株和 1 个 blaCTX-M-1 阳性分离株通过共轭作用转移到受体大肠埃希菌 RG488 中。发现的主要转座单位是 blaCTX-M-55-orf477（45.7%，16/35），其次是 blaCTX-M-65-IS903（28.6%，10/35）和 blaCTX-M-14-IS903（17.1%，6/35）。耐头孢噻呋的伤寒杆菌有 19 个类型，其中 P1-19 型（22.9%，8/35）和 P12-34 型（22.9%，8/35）占多数，在全国大多数养殖场均有发现。不同动物种类的序列类型（ST）各不相同：ST19（48.6%，17/35）和ST34（42.9%，15/35）分别是猪和牛的主要序列类型。这些结果表明，食用动物，尤其是猪和牛，是携带 blaCTX-M 的鼠伤寒沙门氏菌的贮藏地，有可能传播给人类。

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来源期刊

Foodborne pathogens and disease 医学-食品科技

CiteScore

5.30

自引率

3.60%

发文量

审稿时长

1 months

期刊介绍： Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.