Antibiotic Resistance and Genomic Analysis of Shiga Toxin-Producing Escherichia coli from Dairy Cattle, Raw Milk, and Farm Environment in Shaanxi Province, China.

IF 1.9 2区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY

Foodborne pathogens and disease Pub Date : 2024-10-01 Epub Date: 2024-07-23 DOI:10.1089/fpd.2023.0098

Pengfei Zhang, Lisha Liu, Huanjing Sheng, Meng Zhang, Ting Wang, Guanhong Chang, Yeru Wang, Li Bai, Xin Wang

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Abstract

To investigate the epidemiology of Shiga toxin-producing Escherichia coli (STEC) in dairy cattle, 975 samples (185 feces, 34 silage, 36 cattle drinking water, 360 raw milk, and 360 teat skin swabs) were collected from two dairy farms in Baoji and Yangling, Shaanxi Province, China, and were screened for STEC. Whole-genome sequencing was used to analyze the genomic characteristics and potential transmission of STEC isolates. A total of 32 samples were contaminated with STEC, including 4.0% (19/479) in Farm A and 2.6% (13/496) in Farm B. Compared with adult cows (4.5%), nonadult cows had a higher rate (21.3%) of STEC colonization. A total of 14 serotypes and 11 multilocus sequence typing were identified in 32 STEC isolates, among which O55:H12 (25.0%) and ST101 (31.3%) were the most predominant, respectively. Six stx subtypes/combinations were identified, including stx1a (53.1%), stx2g (15.6%), stx2d, stx2a+stx2d, stx1a+stx2a (6.3%, for each), and stx2a (3.1%). Of 32 STEC isolates, 159 virulence genes and 27 antibiotic resistance genes were detected. Overall, STEC isolates showed low levels of resistance to the 16 antibiotics tested (0-40.6%), with most common resistance to ampicillin (40.6%). The phylogenetic analysis confirmed that STEC in the gut of cattle can be transmitted through feces. The results of this study help to improve our understanding of the epidemiological aspects of STEC in dairy cattle and provide early warning and control of the prevalence and spread of the bacterium.

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中国陕西省奶牛、生鲜乳和牧场环境中产志贺毒素大肠杆菌的抗生素耐药性和基因组分析。

为了研究奶牛中产志贺毒素大肠杆菌（STEC）的流行病学，研究人员从中国陕西省宝鸡和杨凌的两个奶牛场收集了 975 份样本（185 份粪便、34 份青贮饲料、36 份牛饮用水、360 份生鲜乳和 360 份乳头皮拭子），并对这些样本进行了 STEC 筛查。采用全基因组测序分析了 STEC 分离物的基因组特征和潜在传播途径。与成年奶牛（4.5%）相比，非成年奶牛的 STEC 定植率更高（21.3%）。在 32 个 STEC 分离物中共鉴定出 14 个血清型和 11 个多焦点序列分型，其中以 O55:H12 （25.0%）和 ST101 （31.3%）最为主要。鉴定出六种stx亚型/组合，包括stx1a（53.1%）、stx2g（15.6%）、stx2d、stx2a+stx2d、stx1a+stx2a（各占6.3%）和stx2a（3.1%）。在 32 个 STEC 分离物中，检测到 159 个毒力基因和 27 个抗生素耐药基因。总体而言，STEC 分离物对所检测的 16 种抗生素的耐药性水平较低（0-40.6%），其中对氨苄西林的耐药性最为常见（40.6%）。系统发育分析证实，牛肠道中的 STEC 可通过粪便传播。这项研究的结果有助于提高我们对奶牛 STEC 流行病学方面的认识，并对该细菌的流行和传播提供预警和控制。

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来源期刊

Foodborne pathogens and disease 医学-食品科技

CiteScore

5.30

自引率

3.60%

发文量

审稿时长

1 months

期刊介绍： Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.