Foodborne pathogens and disease最新文献

{"title":"An Investigation of <i>Salmonella</i> Senftenberg Illnesses in the United States Linked to Peanut Butter-2022.","authors":"Brooke M Whitney, Alexandra Palacios, Benjamin Warren, Donald Kautter, E Ashley Grant, Alvin Crosby, Sharon Seelman, Lindsay Walerstein, Julia Mangia, Arthur Pightling, Allison Hunter, Kerri Harris-Garner, Victoria Wagoner, Timothy Jackson, Lauren Gollarza, Molly Leeper, Laura Gieraltowski, Stelios Viazis","doi":"10.1089/fpd.2024.0089","DOIUrl":"10.1089/fpd.2024.0089","url":null,"abstract":"<p><p>In 2022, the U.S. Food and Drug Administration (FDA), the Centers for Disease Control and Prevention (CDC), and state partners conducted a sample-initiated investigation of a multistate outbreak of <i>Salmonella</i> Senftenberg illnesses linked to peanut butter. Twenty-one illnesses and four hospitalizations were reported in 17 states, with a significant epidemiological signal for peanut butter from Firm A. Whole genome sequence (WGS) data from a <i>Salmonella</i>-positive environmental swab sample collected at Firm A in 2010 yielded the outbreak strain that was a match to the WGS data from the 2022 clinical isolates. Lot code information collected from patients indicated Firm A's facility in Kentucky as a common manufacturing source, and FDA and state partners initiated an inspection. In 2021, Firm A installed two new roasters with at least one of the cooling air supply vents leaking, allowing unfiltered air and rainwater to enter the cooling section after the roasting process. Investigators noted the limitations of Firm A's finished product testing program to identify contamination. Investigative partners from five states collected and analyzed 14 product samples, and FDA collected 205 environmental swabs, and all were negative. Although the exact source and route of the contamination were not determined, epidemiological and traceback evidence confirmed peanut butter consumed by patients was produced by Firm A. Firm A voluntarily recalled all implicated products and provided a plan for corrective actions and restart to FDA. This was the first major domestic investigation of a multistate-foodborne illness outbreak linked to peanut butter since 2012. This investigation demonstrates the importance of caution with reliance on finished product testing, taking appropriate corrective actions when detection occurs, and potential benefits for industry to incorporate WGS as a tool in their environmental monitoring program.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142767798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiomics-Based Biocargo Components Analysis in <i>Enterococcus faecium</i> Membrane Vesicles.","authors":"Meiying Luo, Yuanyuan Zhu, Xiaofang Zhang, Junhang Sun, Xin Feng, Huihua Zhang, Qien Qi","doi":"10.1089/fpd.2024.0031","DOIUrl":"10.1089/fpd.2024.0031","url":null,"abstract":"<p><p><i>Enterococcus</i> spp. have been shown to have gastrointestinal tract protective functions; our recent results suggest that membrane vesicles (MVs) play an important role in the gastric protection of <i>Enterococcus faecium</i> (<i>E. faecium</i>). The specific function is determined by molecular compositions of MVs. To resolve biocargo components in <i>E. faecium</i> MVs (<i>Efm</i>MVs), MVs were isolated from <i>E. faecium</i> culture. Transcriptomics, label-free quantitative proteomics, and untargeted metabolomics were performed to obtain information about the complexity of ribonucleic acids (RNAs), proteins, and metabolites biocargo they carry, respectively. RNA-sequencing identified a total of 2122 transcripts. The top 20 transcripts accounted for 27.63% of total counts, which, including enzymes, participate in glycolysis, ribosomal proteins, DNA-directed RNA polymerases, protein-synthesizing relative enzymes, molecules associated with protein post-translational processing and transport, and peptidoglycan lyases. Label-free quantitative proteomics analysis identified a total of 711 proteins. The top 20 proteins accounted for 48.02% of all identified proteins, which including ribosomal proteins, enzymes participate in glycolysis, DNA-directed RNA polymerases, protein-synthesizing relative enzymes, peptidoglycan lyases, and autolysin. Untargeted metabolomics analysis identified a total of 519 metabolites. The top 20 metabolites accounted for 79.55% of all identified metabolites, which included amino acids, substrates, or products in the metabolism of amino acids, natural organic acids, products in the metabolism of organic acids, ketone compounds, and two other compounds. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses indicated that the identified biocargo components enriched in metabolism, genetic, and environmental information processing. Overall, we hope that the current exploration of multiple \"-omics\" analyses of this <i>Efm</i>MVs will provide useful information and further groundwork for future studies on <i>E. faecium</i> application.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"752-765"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Genomic Investigation of <i>Salmonella</i> Isolates Associated with Watermelons and Their Environmental Reservoirs in Bejaia, Algeria.","authors":"Zahra Bellil, Sylvain Meyer, Valentin Tilloy, Assia Mairi, Christophe De Champs, Olivier Barraud, Abdelaziz Touati","doi":"10.1089/fpd.2023.0045","DOIUrl":"10.1089/fpd.2023.0045","url":null,"abstract":"<p><p>This study was conducted in Bejaia, Algeria, to determine the presence of <i>Salmonella</i> in fresh watermelon (<i>n</i> = 105), soil (<i>n</i> = 23), and irrigation water samples (<i>n</i> = 17) collected from two different farms. After isolation, antimicrobial susceptibility testing, serotype determination, multilocus sequence typing, antimicrobial resistance genes detection, and whole genome sequencing were performed. Twenty watermelon samples (19%) were contaminated with <i>Salmonella</i>, but none were found in the soil or irrigation water. Among the 20 <i>Salmonella</i> isolates, 2 serovars were identified (<i>Salmonella</i> Liverpool and <i>Salmonella</i> Anatum), belonging to sequence types ST1959 and ST64, respectively. Ten <i>Salmonella</i> isolates showed significant resistance to nalidixic acid, ofloxacin, and ciprofloxacin but were susceptible to all other antibiotics. The coexistence of point mutations (<i>parC</i>:p.T57S) in Quinolone Resistance-Determining Regions and the <i>qnrB19</i> gene may contribute to quinolone resistance. The study identified 164 virulence genes in the <i>Salmonella</i> isolates. Our study found <i>Salmonella</i> in fresh watermelon during the preharvest season in Bejaia, Algeria. Our study indicates a relatively high prevalence of <i>Salmonella</i> on watermelon samples before harvest. Although we cannot directly compare our results with previous studies, it is crucial to recognize that the absence of comprehensive comparative data underscores the need for further research and surveillance.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"774-782"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140174099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Burden and Cost of <i>Campylobacter</i> Risk Factors in Australia.","authors":"Danielle M Cribb, Kathryn Glass, Emily L Lancsar, Russell J Stafford, Rhiannon L Wallace, Martyn D Kirk, Angus T McLure","doi":"10.1089/fpd.2024.0022","DOIUrl":"10.1089/fpd.2024.0022","url":null,"abstract":"<p><p><i>Campylobacter</i> is a globally important pathogen with well-studied risk factors, but the burden of risk factors has not been quantified. We quantified the cost of illness attributable to specific domestic risk factors for <i>C. jejuni</i> and <i>C. coli</i> in Australia. We used data from a 2018-2019 case-control study to estimate odds ratios and attributable fractions for risk factors. We used data on national incidence, hospitalization, and premature mortality to quantify burden. We then applied costs related to healthcare utilization, pain and suffering, premature mortality, and lost productivity to each risk factor. In Australia, <i>C. jejuni</i> caused 83.0% of campylobacteriosis infections and chicken consumption resulted in the highest attributable fraction (30.0%), costing approximately US$110 million annually. The excess burden of campylobacteriosis associated with the use of proton-pump inhibitors (PPIs) was US$45 million, with almost half these costs due to disease in adults over 65 years of age. Contact with young dogs (US$30 million) and chicken feces (US$10 million) also contributed to costs and burden. Campylobacteriosis is a significant cost to Australia, particularly because of lost productivity. Effective cross-sectoral interventions to improve chicken meat safety and reduce inappropriate use of PPIs might have substantial economic and human benefits.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"745-751"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Polymyxin Resistance Gene <i>mcr-10</i> from Different Food Samples in Shanghai, China.","authors":"Zhang Hongzhi, Chen Wenjie, Lu Xiaoyu, Liang Yingying, Xiao Quan, Shi Tianqi, Yu Ying, Li Ruichao, Wu Huanyu","doi":"10.1089/fpd.2024.0049","DOIUrl":"10.1089/fpd.2024.0049","url":null,"abstract":"<p><p>This retrospective investigation (2019-2022) identified two plasmid-mediated <i>mcr-10</i> from 6800 food samples in Shanghai, China and localized in a conjugative plasmid (pEC1918-mcr10) in <i>Escherichia kobei</i> from ready-to-eat food with high-level polymyxin B resistance, and a nonconjugative plasmid (pEC2001-mcr10) in <i>E. coli</i> from chicken. These genes were adjacent to IS<i>Ec36</i>. This report highlights the emergence of <i>mcr-10</i> from food samples in Shanghai, China. Active surveillance of vital resistance genes along food production chain should be performed.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"783-786"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142344477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial Activity and Mechanism of Lauric Acid Against <i>Staphylococcus aureus</i> and Its Application in Infectious Cooked Chicken.","authors":"Liu Qingyan, Shi Susu, Liu Shuanglin, Xian Youhua, Yan Haiyang, Yuan Yuan","doi":"10.1089/fpd.2024.0063","DOIUrl":"10.1089/fpd.2024.0063","url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> contamination and prevention has always been a major concern for food industry. This work investigated the antibacterial activity and mechanisms of lauric acid (LA) against <i>S. aureus</i>. Results revealed 156 μg/mL was the minimum inhibitory concentration (MIC) for LA and it retarded growth rate of <i>S. aureus</i>. The inhibitory effect was enhanced with LA concentration. After being treated with 2 MIC LA for 24 h, the number of <i>S. aureus</i> decreased by 3.56 log colony-forming unit (CFU)/mL. Scanning electron microscopy profiling revealed that LA resulted in altered morphology of <i>S. aureus</i> cells. In addition, propidium iodide staining of flow cytometry suggested that LA treatment disrupted the cell membrane integrity. Changes in 8-anilino-1-naphthalenesulfonic acid fluorescence indicated a depolarization change in cell membrane fluidity. For practical applications, LA also displayed an antimicrobial potential in cooked chicken food model system, with 1.25-5 g/L of LA prolonging shelf life by 2 days at 4°C. Moreover, it had no adverse effect on pH values, color in cooked chicken meat, and even reduced lipid oxidation. To sum up, LA has great antimicrobial properties and is a candidate preservative for cooked meat food.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"766-773"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142125264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rotavirus Infections: A 2-Year Comprehensive Review in Admitted Pediastric Patients Amid Conflicting National Policies.","authors":"Yusuf Deniz, Burak Demircioğlu, İrem Kuter-Emeklioğlu, Sadrettin Ekmen, Eylem Sevınc, Erkan Dogan","doi":"10.1089/fpd.2024.0019","DOIUrl":"10.1089/fpd.2024.0019","url":null,"abstract":"<p><p>Highlighting rotavirus (RV) as a significant food and waterborne pathogen, particularly affecting infants and children, causing serious gastrointestinal infections and dehydration, is important. It should be noted that there are significant debates regarding the effectiveness and outcomes of RV vaccination. In contrast to Turkey's nonmandatory vaccination policy, many developed countries implement mandatory vaccination policies, raising questions about their impact on disease prevalence and healthcare expenditures. Our study aims to comprehensively understand RV infections in Turkey and compare them with countries that have mandatory vaccination policies. We found similar, and even better, hospitalization rates, length of hospital stays, and laboratory parameters demonstrating the effectiveness of Turkey's independent vaccination approach. These findings contribute valuable insights to global vaccination strategies and disease control.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"738-744"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142055334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of Non-<i>Saccharomyces</i> Yeasts as Probiotics and Alternatives to Antibiotics in Animal Production.","authors":"Djamel Drider, Vanessa Demey, Giuseppe Spano, Françoise Coucheney, Frédérique Chaucheyras-Durand, Mathieu Castex","doi":"10.1089/fpd.2023.0175","DOIUrl":"10.1089/fpd.2023.0175","url":null,"abstract":"<p><p>Probiotics are live yeast or bacterial organisms that have beneficial effects on the host. Several microorganisms exhibit probiotic properties, the most common types being lactic acid bacteria, <i>Bifidobacteria</i>, spore-forming bacteria, and some yeast strains. <i>Saccharomyces cerevisiae</i> var. <i>boulardii</i> is the most important probiotic yeast species. However, another group of foodborne microorganisms, the so-called non-<i>Saccharomyces</i> yeasts (NSYs), has recently been re-evaluated and shown to have enormous potential in various fields of application, ranging from food fermentation to human and animal applications. NSYs are able to produce a range of bioactive compounds such as antimicrobials, mannoproteins, enzymes, polyunsaturated fatty acids, essential amino acids, vitamins, and β-glucans, which increases their potential applications as a new class of probiotics and/or alternatives to antibiotics in animal husbandry. In this review, we aim to highlight the potential and benefits of NSYs as probiotics and natural antimicrobials to improve animal health. Furthermore, the use of NSYs as biological alternatives to antibiotics to control foodborne pathogens in animal production is discussed.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"731-737"},"PeriodicalIF":1.9,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142046552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic Characterization and Comparative Genomic Analysis of the Foodborne <i>Burkholderia gladioli</i> pv. <i>cocovenenans</i>.","authors":"Jing Chen, Xiaoqing Liu, Bin Liu, Qiongying Yan, Leshi Li, Jie Wang, Yanhui Wu, Chengrong Xiao, Guangzong Xie, Zeyu Lin, Xintian Lai, Jianfei Huang","doi":"10.1089/fpd.2024.0011","DOIUrl":"https://doi.org/10.1089/fpd.2024.0011","url":null,"abstract":"<p><p>The <i>Burkholderia gladioli</i> pv. <i>cocovenenans</i> (<i>B. cocovenenans</i>) has been linked to fatal food poisoning cases, which could produce the deadly toxin of bongkrekic acid (BA). However, genomic characterization and toxin production pathways of <i>B. cocovenenans</i> strains remain elusive. This study aimed to explore the BA-producing ability associated with the evolution of the <i>bon</i> gene cluster and to analyze the intraspecies genomic diversity and phylogenetic relationships of <i>B. gladioli</i> based on the 17 genomes of <i>B. cocovenenans</i> strains isolated from Shenzhen City, China. Genome sequencing results suggested that the genome sizes of these <i>B. cocovenenans</i> strains were mostly approximately 8 Mb, with a GC content of approximately 68%. The evolutionary tree analysis of the whole-genome sequences showed that significant divergences and distinct cluster were exhibited among these <i>B. cocovenenans</i> strains. Comparative genomic analysis indicated that the genomes of strains 2020051, 2021031, and 2021067 contained the complete and entire <i>bon</i> gene cluster, supporting that these strains displayed obviously BA-producing ability. The genomes of strains 2021028 and 2020041 lacked the entire <i>bon</i> gene cluster. However, the genomes of strains 2021037, 2021024, 2021035, and 2021031 exhibited disruptions in their <i>bon</i> gene clusters. This finding indicated the loss of specific genes within the cluster, suggesting a reduced capability for BA production in these strains. The present results indicated that the <i>bon</i> gene cluster in the genome played a key role in the toxin BA biosynthesis of different <i>B. cocovenenans</i> strains. This study provided a comprehensive understanding of the relationship between genomic diversity and BA production of this lethal foodborne pathovar, which will potentially contribute to the risk identification and food poisoning outbreak prevention of <i>B. cocovenenans</i>.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142727330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Biofilm Formation Ability and Antibiotic Resistance of <i>Staphylococcus aureus</i> Isolates from Food Products.","authors":"Yacine Titouche, Madjid Akkou, Yasmina Djaoui, Achour Chergui, Donia Mechoub, Lamia Bentayeb, Abdelhak Fatihi, Yacine Nia, Jacques-Antoine Hennekinne","doi":"10.1089/fpd.2024.0121","DOIUrl":"10.1089/fpd.2024.0121","url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> is one of the major causes of foodborne diseases and its presence in food products may poses a public health challenge. The aims of this study were to assess <i>in vitro</i> the capacity of <i>S. aureus</i> isolates from foods to form biofilm and to determine their antibiotic susceptibility. A total of 80 <i>S. aureus</i> isolates were characterized. The slime production ability was evaluated by congo-red agar (CRA) and the biofilm formation was carried out by microtiter-plate method (MPM). Resistance of isolates to eight antibiotics was determined using disc diffusion method. Sixty-four (80%) of the isolates were slime producers on congo-red agar. However, all isolates were biofilm producers on microtiter-plate method. The highest resistance profiles were ascribed to penicillin G (91.25%) and tetracycline (41.25%). Twelve isolates were methicillin-resistant (MRSA) harboring the <i>mecA</i> gene. All of these MRSA isolates were negative for the genes of the Panton Valentine leukocidine (<i>lukF/S</i>-PV). Typing of the MRSA isolates indicated that they belonged to three spa-types including t024, t450 and t688. The presence of biofilm producers and multidrug resistant isolates (MRSA) in food samples can represent a risk for public health. Therefore, an efficient control and effective measures were needed along the production chain to ensure the food safety.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}