Foodborne pathogens and disease最新文献

{"title":"Molecular Epidemiology of Carbapenemase-Producing <i>Pseudomonas aeruginosa</i>: An Iranian Referral Hospital-Based Study.","authors":"Shima Mahmoudi, Erfaneh Jafari, Babak Pourakbari, Shiva Poormohammadi, Reihaneh Hosseinpour Sadeghi, Setareh Mamishi","doi":"10.1089/fpd.2024.0066","DOIUrl":"https://doi.org/10.1089/fpd.2024.0066","url":null,"abstract":"<p><p>In recent years, there has been a significant increase in infections caused by carbapenemase-producing strains, with carbapenem-resistant <i>Pseudomonas aeruginosa</i> (CRPA) emerging as a priority pathogen according to the World Health Organization. This study aimed to evaluate the molecular epidemiology of CRPA isolated from patients referred to Children's Medical Center in Tehran, Iran. <i>P. aeruginosa</i> isolates collected from different children's wards were screened for common carbapenem-resistant genes by polymerase chain reaction (PCR). Genetic relatedness between isolates was assessed by pulsed-field gel electrophoresis (PFGE). The study included 133 participants, with 50% being male, and revealed a median age of 2 years (interquartile range: 6 months to 6 years). Carbapenem resistance was detected in 15% of cases (<i>n</i> = 20), with CRPA isolates predominantly found in the emergency ward (60%). The median age of patients with CRPA was significantly higher than those with carbapenem-susceptible <i>P. aeruginosa</i> (6 years vs. 1 year). PCR analysis revealed metallo-β-lactamase production in 45% of CRPA isolates (<i>n</i> = 9), with <i>bla_NDM</i> being the most prevalent gene. PFGE analysis of the CRPA isolates identified three clusters (Cluster I, II, and III). Cluster I, comprising 65% of all isolates (<i>n</i> = 13), was predominantly found in the emergency ward. Notably, <i>bla_NDM</i>-producing strains were prevalent in the emergency ward. Our study highlights the significant prevalence of CRPA in the emergency ward of our hospital and underscores the importance of targeted surveillance and infection control measures to curb its spread within health care settings.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Burden and Cost of <i>Campylobacter</i> Risk Factors in Australia.","authors":"Danielle M Cribb, Kathryn Glass, Emily L Lancsar, Russell J Stafford, Rhiannon L Wallace, Martyn D Kirk, Angus T McLure","doi":"10.1089/fpd.2024.0022","DOIUrl":"10.1089/fpd.2024.0022","url":null,"abstract":"<p><p><i>Campylobacter</i> is a globally important pathogen with well-studied risk factors, but the burden of risk factors has not been quantified. We quantified the cost of illness attributable to specific domestic risk factors for <i>C. jejuni</i> and <i>C. coli</i> in Australia. We used data from a 2018-2019 case-control study to estimate odds ratios and attributable fractions for risk factors. We used data on national incidence, hospitalization, and premature mortality to quantify burden. We then applied costs related to healthcare utilization, pain and suffering, premature mortality, and lost productivity to each risk factor. In Australia, <i>C. jejuni</i> caused 83.0% of campylobacteriosis infections and chicken consumption resulted in the highest attributable fraction (30.0%), costing approximately US$110 million annually. The excess burden of campylobacteriosis associated with the use of proton-pump inhibitors (PPIs) was US$45 million, with almost half these costs due to disease in adults over 65 years of age. Contact with young dogs (US$30 million) and chicken feces (US$10 million) also contributed to costs and burden. Campylobacteriosis is a significant cost to Australia, particularly because of lost productivity. Effective cross-sectoral interventions to improve chicken meat safety and reduce inappropriate use of PPIs might have substantial economic and human benefits.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Prevalence of <i>Brucella</i> Spp. in Dairy Products in Iran: A Systematic Review and Meta-Analysis.","authors":"Narges Shahbazpour, Elahesadat Hosseini, Seyed Ehsan Beladian Behbahan, Fatemeh Esfarjani, Seyed Amir Mohamad Mortazavian, Abdol-Samad Abedi, Slim Smaoui","doi":"10.1089/fpd.2024.0093","DOIUrl":"https://doi.org/10.1089/fpd.2024.0093","url":null,"abstract":"<p><p>Brucellosis, a significant zoonotic disease, threatens food safety substantially, particularly in developing nations such as the Middle East. This study aimed to comprehensively assess the prevalence of <i>Brucella</i> spp. in Iranian milk and dairy products through a systematic review and meta-analysis. A thorough search of international and domestic databases from January 2008 to October 2023 identified 38 relevant studies encompassing 11,130 samples for meta-analysis. The overall prevalence of <i>Brucella</i> spp. in Iranian dairy products was 22% (95% CI: 16-28%). The highest and lowest overall prevalence of <i>Brucella</i> spp. in milk were found in raw goat milk 27% (95% CI: 11-42%) and raw camel milk 15% (95% CI: -0.42 to 72%), respectively. The overall prevalence of traditional cheese, ice cream, and cream is estimated to be 9% (95% CI: -16 to 35%), 2% (95% CI: -2.78 to 2.82%), and 9% (95% CI: -0.94 to 1.12%). Geographical disparities were evident, with Zanjan province reporting the highest contamination rate, 53%, while Razavi Khorasan province had the lowest, 1%. However, the prevalence of <i>Brucella</i> spp. in Iranian dairy products has fluctuated over time, with a significant association between the study year and sample size. Comprehensive planning, robust policy implementation, and rigorous monitoring are imperative to mitigate and ultimately eliminate <i>Brucella</i> contamination in dairy products effectively. Further research is essential to refine prevalence estimates and develop targeted prevention strategies to safeguard public health.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of Non-<i>Saccharomyces</i> Yeasts as Probiotics and Alternatives to Antibiotics in Animal Production.","authors":"Djamel Drider, Vanessa Demey, Giuseppe Spano, Françoise Coucheney, Frédérique Chaucheyras-Durand, Mathieu Castex","doi":"10.1089/fpd.2023.0175","DOIUrl":"https://doi.org/10.1089/fpd.2023.0175","url":null,"abstract":"<p><p>Probiotics are live yeast or bacterial organisms that have beneficial effects on the host. Several microorganisms exhibit probiotic properties, the most common types being lactic acid bacteria, <i>Bifidobacteria</i>, spore-forming bacteria, and some yeast strains. <i>Saccharomyces cerevisiae</i> var. <i>boulardii</i> is the most important probiotic yeast species. However, another group of foodborne microorganisms, the so-called non-<i>Saccharomyces</i> yeasts (NSYs), has recently been re-evaluated and shown to have enormous potential in various fields of application, ranging from food fermentation to human and animal applications. NSYs are able to produce a range of bioactive compounds such as antimicrobials, mannoproteins, enzymes, polyunsaturated fatty acids, essential amino acids, vitamins, and β-glucans, which increases their potential applications as a new class of probiotics and/or alternatives to antibiotics in animal husbandry. In this review, we aim to highlight the potential and benefits of NSYs as probiotics and natural antimicrobials to improve animal health. Furthermore, the use of NSYs as biological alternatives to antibiotics to control foodborne pathogens in animal production is discussed.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142046552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rotavirus Infections: A 2-Year Comprehensive Review in Admitted Pediastric Patients Amid Conflicting National Policies.","authors":"Yusuf Deniz, Burak Demircioğlu, İrem Kuter-Emeklioğlu, Sadrettin Ekmen, Eylem Sevınc, Erkan Dogan","doi":"10.1089/fpd.2024.0019","DOIUrl":"https://doi.org/10.1089/fpd.2024.0019","url":null,"abstract":"<p><p>Highlighting rotavirus (RV) as a significant food and waterborne pathogen, particularly affecting infants and children, causing serious gastrointestinal infections and dehydration, is important. It should be noted that there are significant debates regarding the effectiveness and outcomes of RV vaccination. In contrast to Turkey's nonmandatory vaccination policy, many developed countries implement mandatory vaccination policies, raising questions about their impact on disease prevalence and healthcare expenditures. Our study aims to comprehensively understand RV infections in Turkey and compare them with countries that have mandatory vaccination policies. We found similar, and even better, hospitalization rates, length of hospital stays, and laboratory parameters demonstrating the effectiveness of Turkey's independent vaccination approach. These findings contribute valuable insights to global vaccination strategies and disease control.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142055334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiomics-Based Biocargo Components Analysis in <i>Enterococcus faecium</i> Membrane Vesicles.","authors":"Meiying Luo, Yuanyuan Zhu, Xiaofang Zhang, Junhang Sun, Xin Feng, Huihua Zhang, Qien Qi","doi":"10.1089/fpd.2024.0031","DOIUrl":"https://doi.org/10.1089/fpd.2024.0031","url":null,"abstract":"<p><p><i>Enterococcus</i> spp. have been shown to have gastrointestinal tract protective functions; our recent results suggest that membrane vesicles (MVs) play an important role in the gastric protection of <i>Enterococcus faecium</i> (<i>E. faecium</i>). The specific function is determined by molecular compositions of MVs. To resolve biocargo components in <i>E. faecium</i> MVs (<i>Efm</i>MVs), MVs were isolated from <i>E. faecium</i> culture. Transcriptomics, label-free quantitative proteomics, and untargeted metabolomics were performed to obtain information about the complexity of ribonucleic acids (RNAs), proteins, and metabolites biocargo they carry, respectively. RNA-sequencing identified a total of 2122 transcripts. The top 20 transcripts accounted for 27.63% of total counts, which, including enzymes, participate in glycolysis, ribosomal proteins, DNA-directed RNA polymerases, protein-synthesizing relative enzymes, molecules associated with protein post-translational processing and transport, and peptidoglycan lyases. Label-free quantitative proteomics analysis identified a total of 711 proteins. The top 20 proteins accounted for 48.02% of all identified proteins, which including ribosomal proteins, enzymes participate in glycolysis, DNA-directed RNA polymerases, protein-synthesizing relative enzymes, peptidoglycan lyases, and autolysin. Untargeted metabolomics analysis identified a total of 519 metabolites. The top 20 metabolites accounted for 79.55% of all identified metabolites, which included amino acids, substrates, or products in the metabolism of amino acids, natural organic acids, products in the metabolism of organic acids, ketone compounds, and two other compounds. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses indicated that the identified biocargo components enriched in metabolism, genetic, and environmental information processing. Overall, we hope that the current exploration of multiple \"-omics\" analyses of this <i>Efm</i>MVs will provide useful information and further groundwork for future studies on <i>E. faecium</i> application.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contamination Status and Serotypes Distribution of <i>Salmonella</i> in Food in Yantai City, China: A 14-Year Continuous Monitoring Study.","authors":"Fengguang Dong, Guiqiang Wang, Xueying Feng, Chunbo Gong, Wenjuan Liu, Songsong Wang, Yiyi Zhang, Yapeng Huo, Youxia Chen, Hongtao Wang","doi":"10.1089/fpd.2024.0055","DOIUrl":"https://doi.org/10.1089/fpd.2024.0055","url":null,"abstract":"<p><p><i>Salmonella</i> is a foodborne zoonotic pathogen that threatens food safety and public health. However, few people have conducted long-term and systematic studies on <i>Salmonella</i> contamination in food in Yantai City. In order to investigate the situation of <i>Salmonella</i> contamination in food and improve the ability of early warning and control of foodborne diseases, a total of 3420 samples from 20 categories were collected from 13 monitoring points in Yantai City, from 2010 to 2023. The difference in detection rate and bacterial strain of different monitoring points, different types, and different sources of samples was compared. Of the 3420 samples, 80 were positive with a detection rate of 2.34%. <i>Salmonella</i> detection rates were significantly different for samples collected at different monitoring sites. <i>Salmonella</i> was detected only in meat and meat products and catering food, and none of the other types were detected. The detection rate of <i>Salmonella</i> was higher in raw animal meat and raw poultry. Samples collected at the market stage had the highest detection rate (5.81%), and there was a significant difference in detection rate between samples from different sources (χ² = 36.93, <i>p</i> < 0.05). Eighty-one strains of <i>Salmonella</i> were detected out of 3420 samples (2 different strains were detected in 1 positive sample). The serological test identified 8 groups and 27 serotypes. The dominant serum groups were group B 30.86% (25/81), group E1 23.46% (19/81), and group D 16.05% (13/81). The main dominant serotypes were <i>Salmonella</i> <i>give</i> 17.28% (14/81), <i>Salmonella</i> <i>enteritidis</i> 16.05% (13/81), and <i>Salmonella</i> <i>derby</i> 13.58% (11/81). Meat and meat products and catering food were the main food products contaminated with <i>Salmonella</i>. The resulting secondary contamination is the hidden threat of foodborne diseases and should be given sufficient attention.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141909835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Duplex PCR Assay for Rapid Detection of <i>Klebsiella pneumoniae</i> and <i>Chryseobacterium</i> in Large Yellow Croaker Fish.","authors":"Gaowei Hu, Longfei Yin, Xi Luo, Yingjie Miao, Jianyun Yu","doi":"10.1089/fpd.2023.0149","DOIUrl":"10.1089/fpd.2023.0149","url":null,"abstract":"<p><p>Both <i>Klebsiella pneumoniae</i> and <i>Chryseobacterium</i> cause an increasing number of diseases in fish, resulting in great economic losses in aquaculture. In addition, the disease infected with <i>Klebsiella pneumoniae</i> or <i>Chryseobacterium</i> exhibited the similar clinical symptoms in aquatic animals. However, there is no effective means for the simultaneous detection of co-infection and discrimination them for these two pathogens. Here, we developed a duplex polymerase chain reaction (PCR) method based on the outer membrane protein A (<i>ompA</i>) gene of <i>Klebsiella pneumoniae</i> and <i>Chryseobacterium</i>. The specificity and validity of the designed primers were confirmed experimentally using simplex PCR. The expected amplicons for <i>Klebsiella pneumoniae</i> and <i>Chryseobacterium</i> had a size of 663 and 1404 bp, respectively. The optimal condition for duplex PCR were determined to encompass a primer concentration of 0.5 μM and annealing temperature of 57°C. This method was analytical specific with no amplification being observed from the genomic DNA of <i>Escherichia coli</i>, <i>Vibrio harveyi</i>, <i>Pseudomonas plecoglossicida</i>, <i>Aeromonas hydrophila</i> and <i>Acinetobacter johnsonii</i>. The limit of detection was estimated to be 20 fg of genomic DNA for <i>Chryseobacterium</i> and 200 fg for <i>Klebsiella pneumoniae</i>, or 100 colony-forming units (CFU) of bacterial cells in both cases. The duplex PCR was capable of simultaneously amplifying target fragments from genomic DNA extracted from the bacteria and fish liver. For practical validation of the method, 20 diseased fish were collected from farms, among which 4 samples were PCR-positive for <i>Klebsiella pneumoniae</i> and <i>Chryseobacterium</i>. The duplex PCR method developed here is time-saving, specific, convenient, and may prove to be an invaluable tool for molecular detection and epidemiological investigation of <i>Klebsiella pneumoniae</i> and <i>Chryseobacterium</i> in the field of aquaculture.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"508-516"},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140850108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Biological Characteristics of a Novel Phage and Its Application to Control <i>Vibrio Parahaemolyticus</i> in Shellfish Meat.","authors":"Wenting Liu, Yiming Wu, Huajuan Wang, Hongxun Wang, Min Zhou","doi":"10.1089/fpd.2023.0133","DOIUrl":"10.1089/fpd.2023.0133","url":null,"abstract":"<p><p><i>Vibrio parahaemolyticus</i> is a common foodborne pathogenic bacterium. With the overuse of antibiotics, an increasing proportion of drug-resistant strains are emerging, which puts enormous pressure on public health. In this study, a <i>V. parahaemolyticus</i>-specific phage, VP41s3, was isolated. The head length, width, and tail length of the phage were 77.7 nm, 72.2 nm, and 17.5 nm, respectively. It remained active in the temperature range of 30-50°C and pH range of 4-11. The lytic curve of phage VP41s3 showed that the host bacteria did not grow until 11 h under phage treatment at MOI of 1000, indicating that the phage had good bacteriostatic ability. When it was added to shellfish contaminated with <i>V. parahaemolyticus</i> (15°C, 48 h), the number of bacteria in the experimental group was 2.11 log₁₀ CFU/mL lower than that in the control group at 24 h. Furthermore, genomic characterization and phylogenetic analysis indicated that phage VP41s3 was a new member of the <i>Podoviridae</i> family. The genome contained 50 open reading frames (ORFs), in which the ORF19 (thymidine kinase) was an enzyme involved in the pyrimidine salvage pathway, which might lead to the accelerated DNA synthesis efficiency after phage entered into host cells. This study not only contributed to the improvement of phage database and the development of beneficial phage resources but also revealed the potential application of phage VP41s3 in food hygiene and safety.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"467-477"},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140957168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of <i>Salmonella</i> Enteritidis into a Viable but Nonculturable State by Cinnamaldehyde and Its Resuscitation.","authors":"Ziying Lu, Tong Xing, Zepeng Zhao, Zefeng Li, Dongping Hou, Zhuolin Ma, Siyi Chen, Yuheng Yang, Shaoting Li, Hongmei Zhang","doi":"10.1089/fpd.2023.0186","DOIUrl":"10.1089/fpd.2023.0186","url":null,"abstract":"<p><p>Trans-cinnamaldehyde (TC), a typical plant-derived compound, has been widely used in the control of foodborne pathogen contamination. Nevertheless, the risk associated with the occurrence of viable but nonculturable (VBNC) bacteria induced by TC remains unclear. The results of this study showed that <i>Salmonella</i> Enteritidis (<i>S.</i> Enteritidis) entered the VBNC state after being induced by TC at a minimum inhibitory concentration of 312.5 μg/mL and survived for at least 22 days under TC treatment. Enhanced resistance was found against heat treatment (75°C, 30 s), antibiotics (i.e., ampicillin, ceftriaxone sodium, chloramphenicol), and hydrogen peroxide (3%) in VBNC <i>S.</i> Enteritidis. A synergistic effect against VBNC <i>S.</i> Enteritidis occurred when TC was combined with acid treatment, including lactic acid and acetic acid (pH = 3.5). VBNC and resuscitated <i>S</i>. Enteritidis by sodium pyruvate treatment (100 mM) were found to retain the infectious ability to Caco-2 cells. Relative expression levels of the stress-related genes <i>relA</i>, <i>spoT</i>, <i>ppx</i>, <i>lon</i>, <i>katG</i>, <i>sodA</i>, <i>dnaK</i>, and <i>grpE</i> were upregulated in VBNC <i>S</i>. Enteritidis. Accumulation of reactive oxygen species (ROS) and protein aggregates was observed in VBNC cells. Besides, the resuscitation of VBNC cells was accompanied with clearance of ROS and protein aggregates. In summary, this study presents a comprehensive characterization of stress tolerance and resuscitation of VBNC <i>S</i>. Enteritidis induced by cinnamaldehyde, and the results provide useful information for the development of effective control strategy against VBNC pathogenic bacteria in food production.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"499-507"},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140853382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}