Foodborne pathogens and disease最新文献

{"title":"Recent Occurrence and Rapid Spread of Multidrug-Resistant <i>Salmonella</i> Infantis in Broiler Flocks in Korea.","authors":"So-Hee Lee, O-Mi Lee, Sung-Il Kang, Moon Her, Min-Su Kang, Myeongju Chae, Min-Goo Seo","doi":"10.1089/fpd.2024.0162","DOIUrl":"https://doi.org/10.1089/fpd.2024.0162","url":null,"abstract":"<p><p><i>Salmonella</i> Infantis has recently been one of the most prevalent serotypes in poultry and has been identified in human salmonellosis cases worldwide. Multidrug-resistant (MDR) <i>Salmonella</i> Infantis has emerged as a significant threat to both poultry production and public health due to its increasing prevalence and global dissemination. We identified the occurrence of an MDR <i>Salmonella</i> Infantis clone in broiler flocks in Korea, and the clone was characterized to explore potential genetic causes for its high prevalence and rapid spread in broiler production. In total, 220 <i>Salmonella</i> strains isolated between 2020 and 2023 from broiler flocks were serotyped, and 50 strains were identified as <i>Salmonella</i> Infantis (22.7%). The isolates were tested for antimicrobial susceptibility, and their genetic characteristics were analyzed using pulsed-field gel electrophoresis (PFGE), multilocus sequence typing, and whole genome sequencing (WGS). Forty-six strains of <i>Salmonella</i> Infantis isolated since 2020 were resistant to at least five antimicrobial families including ampicillin, cephalosporins, chloramphenicol, nalidixic acid, and tetracycline. The strains showed 10 PFGE patterns and a single multilocus sequence type 32. Eight representative MDR strains were analyzed by WGS. Seven of the eight strains carried the plasmid of emerging <i>Salmonella</i> Infantis-like megaplasmids recognized globally in emergent MDR <i>Salmonella</i> Infantis. They had a high prevalence of seven antimicrobial resistance genes, six of which were identified in plasmids. Also, they all share virulence genes, including fimbrial adherence determinants and secretion system components, and showed a clonal relationship to strains from North America, South America, and West Asia, suggesting potential international dissemination routes. To mitigate the risks associated with the rapid spread of MDR <i>Salmonella</i> Infantis in poultry production and its potential impact on human health, this study provides valuable insights into implementing effective control measures to reduce <i>Salmonella</i> in broiler production in Korea. Further highlighting the critical importance of enhanced biosecurity and continuous surveillance.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sporadic Listeriosis Cases, Genotypic Characteristics, and Antibiotic Resistance of Associated <i>Listeria monocytogenes</i> Isolates in Zhejiang Province, China (2016 - 2022).","authors":"Yunyi Zhang, Junyan Zhang, Jiancai Chen, Xiaojuan Qi, Zhen Zhang, Honghu Chen, Beibei Wu, Li Zhan, Jie Chen","doi":"10.1089/fpd.2024.0083","DOIUrl":"https://doi.org/10.1089/fpd.2024.0083","url":null,"abstract":"<p><p><i>Listeria monocytogenes</i> (<i>L. monocytogenes</i>) is an important foodborne pathogen. In this study, 41 sporadic listeriosis cases were collected during 2016-2022, including 92.7% of invasive cases and 56.1% of pregnancy-associated cases. The age of cases ranged from 0 days to 88 years, with the majority occurring in individuals aged 20 to <30 years. Serotype 1/2 b was most prevalent among 43 <i>L. monocytogenes</i> isolates, followed by 1/2 a, 4 b, and 3a. Sixteen clonal complexes (CCs) were determined. CC87 occupied the top slot. Genome sequencing-based phylogeny results indicated that Chinese CC1, CC8, and CC87 isolates mostly clustered in clades separating from isolates from other countries. Meanwhile, a few Chinese isolates participated in cocirculating CC1, CC8, and CC87 in Asia, Europe, Africa, South America, North America, and Oceania. All isolates harbored LIPI-1 while LIPI-2 was absent. LIPI-3 and LIPI-4 exhibited an apparent relationship with lineage and CCs. It was notably that CC4, CC224, and CC619 carried both LIPI-3 and LIPI-4. <i>inlB</i>, <i>inlC</i>, <i>inlH</i>, <i>inlK</i>, <i>ipeA</i>, <i>srtA</i>, <i>dltA</i>, <i>lap</i>, <i>ami</i>, <i>fbpA</i>, <i>stp</i>, <i>oatA</i>, <i>intA</i>, <i>prsA2</i>, <i>lgt</i>, <i>hpt</i>, <i>iplA1</i>, <i>bsh</i>, <i>mdrT</i>, <i>mdrM</i>, and <i>brtA</i> existed in all isolates. The percentages of <i>inlA, inlF, inlJ, aut, vip</i> were 97.7%, 97.7%, 97.7%, 83.7%, and 83.7%. A premature stop codon mutation of position 1474(C→T) was detected, resulting in a truncated InlA with 491 aa. High susceptibility to penicillin (100%), ampicillin (100%), gentamicin (100%), erythromycin (100%), daptomycin (100%), meropenem (100%), trimethoprim-sulfamethoxazole (100%), vancomycin (97.7%), tetacycline (97.7%), chloramphenicol (97.7%), and ciprofloxacin (90.7%) was demonstrated. <i>lin</i> and <i>fosX</i> were present in 93.0% of the isolates, respectively. <i>tetM</i> and <i>ermB</i> were also detected. This comprehensive study enriched the understanding of listeriosis cases and diversity of clinical isolates, meanwhile, indicated the spread characteristics of CC1, CC8, and CC87 in China based on phylogeny analysis, providing fundamental data for developing targeting food safety interventions to prevent listeriosis.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of <i>Giardia</i> in Nine Species of Wild Rodents in Guangxi, Hunan, and Yunan Provinces, China.","authors":"Ya Qin, Zhen-Qiu Gao, Hai-Tao Wang, Qing-Yu Hou, Si-Yuan Qin, Quan Zhao, He Ma, Li-Hua Yang, Shuo Liu","doi":"10.1089/fpd.2024.0185","DOIUrl":"https://doi.org/10.1089/fpd.2024.0185","url":null,"abstract":"<p><p><i>Giardia</i>, a flagellated protozoan, is widely distributed in wild rodents. To characterize the presence of <i>Giardia</i> in wild rodents in southern China, this study collected 344 fecal samples from wild rodents in Guangxi, Hunan, and Yunnan provinces. The β-giardin gene (<i>bg</i>) was amplified to identify the presence of <i>Giardia</i> sp. The results showed that 48 samples (13.95%, 48/344) tested positive for <i>Giardia</i>, including three positives for <i>G. duodenalis</i> (assemblage G <i>n</i> = 1, assemblage F <i>n</i> = 2) and 45 positives for <i>G. microti.</i> Among these, <i>Microtus fortis</i> had the highest prevalence at 30.22% (42/139), followed by <i>Apodemus agrarius</i> (7.14%, 3/42). <i>Rattus flavipectus</i> showed a prevalence of 2.56% (1/39), while <i>Bandicota indica</i> had a rate of 5.13% (2/39). The prevalence of <i>Giardia</i> in males (18.27%, 36/197) was slightly higher than in females (8.16%, 12/147). To the best of our knowledge, the present study reported the detection of assemblage F and assemblage G in <i>B. indica</i> for the first time. In addition, <i>G. microti</i> was found exclusively in <i>M. fortis</i> and a few <i>A. agrarius</i>. This study enriches the epidemiological data on <i>Giardia</i> in wild rodents and provides new insights for future research.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143467472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation and Analysis of <i>Staphylococcus aureus</i> Contamination in Food in Yantai City, China: Based on a 14-Year Continuous Monitoring.","authors":"Fengguang Dong, Guiqiang Wang, Xueying Feng, Chunbo Gong, Zhong Zheng, Youxia Chen, Yapeng Huo, Yiyi Zhang, Hongtao Wang","doi":"10.1089/fpd.2024.0175","DOIUrl":"https://doi.org/10.1089/fpd.2024.0175","url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> is a foodborne zoonotic pathogen that threatens food safety and public health. However, few people have conducted long-term and systematic studies on <i>S. aureus</i> contamination in food in Yantai City. To investigate the contamination situation of <i>S. aureus</i> in food and improve the ability of early warning and control of foodborne diseases, a total of 2384 samples from 17 categories were collected from 13 monitoring points in Yantai City, from 2010 to 2023. Forty-four samples were positively detected for <i>S. aureus</i>, with a detection rate of 1.85% (44/2384). The detection rate of <i>S. aureus</i> was highest in Zhifu District (4.12%), followed by Penglai District (2.45%), Zhaoyuan District (2.37%), Kaifa District (2.19%), and Longkou District (1.98%). Positive detection rates were higher in frozen rice and flour products at 8.82% (6/68), quick-frozen dishes at 5.56% (1/18), aquatic products at 4.05% (3/74), and meat and meat products at 3.55% (27/760). Positive detection rates in samples from the first, second, third, and fourth quarters were 0% (0/44), 2.21% (20/906), 2.13% (22/1033), and 0.50% (2/401), respectively. Positive detection rates in bulk and prepackaged samples were 2.33% (36/1546) and 0.95% (8/838), respectively, with statistically significant differences (<i>χ²</i> = 5.66, <i>p</i> < 0.05). Positive detection rates were significantly different for samples collected from different sampling stages, of which at production and processing stages was 7.78% (20/257), catering stages 1.38% (10/727), and distribution stages 1% (14/1400) (<i>χ²</i> = 56.41, <i>p</i> < 0.05). Frozen rice and flour products, quick-frozen dishes, aquatic products, and meat and meat products are the main food products contaminated with <i>S. aureus</i>, and the resulting secondary contamination is a hidden danger for the occurrence of foodborne diseases, which should be given sufficient attention.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143467470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Controlling Multidrug-Resistant <i>Staphylococcus aureus</i> by Combined Use of Antimicrobials and Phage STPX-6 with Broad Spectrum and High Efficiency.","authors":"Lin Wang, Zhen Xiao, Juan Wang, Na Liu, Wen Jiang, Yaopeng Liu, Fangyuan Hu, Huanqi Liu, Junhui Liu, Zhina Qu, Junwei Wang","doi":"10.1089/fpd.2024.0130","DOIUrl":"https://doi.org/10.1089/fpd.2024.0130","url":null,"abstract":"<p><p><b><i>Objective:</i></b> The emergence of antibiotic-resistant <i>Staphylococcus aureus</i>, especially methicillin-resistant <i>S. aureus</i> (MRSA), poses a great challenge for animal and public health. This study aimed to isolate a broad-spectrum and high-efficiency MRSA phage and explore the phage-antibiotic synergistic effect on MRSA. <b><i>Results:</i></b> Phage STPX-6 belongs to <i>Caudovirales</i>, <i>Podoviridae</i>. It has a hexahedral head and a short tail. Its genome length was 17,007 bp, and it did not contain resistance genes and virulence genes. STPX-6 lysed 79.6% (133/167) of 167 <i>S. aureu</i>s and 87.96% (95/108) of MRSA from different sources. The titer of phage was 1.18 × 10¹⁰ PFU/mL, the optimal multiplicity of infection was 1, the latent period and lysis period were about 10 min and 60 min, respectively, and the burst amount was 68 PFU/cell. At 50°C and 70-90°C, the titer of STPX-6 was maintained at about 10¹⁰ PFU/mL and at least 10³ PFU/mL, respectively. In the range of pH 4-12, the titer of phage remained above 10⁸ PFU/mL, and it remained above 10⁴ PFU/mL at pH 2, 3, 13, and 14. The combined application of phage STPX-6 and enrofloxacin, doxycycline, ampicillin could reduce the minimum inhibitory concentration (MIC) of the three antibiotics to 1/4 MIC, 1/16 MIC, and 1/2 MIC, respectively. <b><i>Conclusion:</i></b> This study found that for the host MRSA, lytic phage STPX-6 had the characteristics of a broad lytic spectrum, a short latent period, strong adaptability and strong tolerance to high temperature, a strong acid and strong alkali environment, and might maintain certain activity under extreme environment. More importantly, the combination of phage STPX-6 with enrofloxacin, doxycycline, and ampicillin could reduce the antibiotic concentration used for MRSA. In other words, phages as new antibacterial agents have received increasing attention. The combined application of phages and antibiotics provides a new method for controlling multidrug resistant bacteria and reduce the use of antibiotics.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143254323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Baitouweng Decoction on <i>Salmonella</i> Typhimurium Infection and Its Underlying Mechanisms Evaluated by <i>In Vivo</i> and <i>In Vitro</i> Experiments, Network Pharmacology Analysis, and Molecular Docking Technology.","authors":"Cui Xinhua, Wang Yang, Shi Jinyang, Xu Hongyue, Yu Wanlu, Zhu Mingmei, Qiu Jiazhang, Yu Lu","doi":"10.1089/fpd.2023.0169","DOIUrl":"10.1089/fpd.2023.0169","url":null,"abstract":"<p><p><i>Salmonella</i> Typhimurium is a foodborne pathogen threatening livestock and human health. It is highly resistant to commonly used clinical antibiotics, and it is urgently needed to explore new anti-<i>Salmonella</i> treatment schemes. In this study, first, our <i>in vivo</i> mouse experiments showed that Baitouweng decoction (BTW), a classical Traditional Chinese Medicine (TCM) prescription, had good efficacy against <i>Salmonella</i> Typhimurium infection: mitigating weight loss of mice; lowering the bacterial load of liver, spleen, and colon; reducing the production of serum inflammatory factors (interleukin-1β and tumor necrosis factor-α); and decreasing histological index scores than that in the <i>Salmonella</i> Typhimurium infection group. Furthermore, we explored the potential active components and molecular mechanism of BTW in the treatment of <i>Salmonella</i> Typhimurium infection. A total of 465 compounds of BTW were retrieved from herb website and 227 bioactive compounds were identified, 911 potential BTW-related targets and 1,602 disease targets of <i>Salmonella</i> Typhimurium infection were acquired by ten public analytical databases, among them, 188 genes were overlay targets of BTW-<i>Salmonella</i> Typhimurium; String, Metascape, and Cytoscape plug-in Molecular Complex Detection and ClueGo analysis pointed that BTW exerted an anti-<i>Salmonella</i> effect through a multicomponent, multitarget, and multipathway manner, including 10 hub targets (TNF, AKT CASP3, ALB, EGFR, JUN, MAPK, STAT3, VEGFA, and TP53) and 94 pathways such as cell apoptosis, inflammation, and metabolism. Finally, AutoDock Vina showed that the hub target AKT1 with menispermine and quercetin had good binding energy, which was confirmed by the <i>in vitro</i> cellular thermal shift assay and drug affinity responsive target stability assay. This study laid the foundation for further study of BTW mechanism and for further development of BTW anti-<i>Salmonella</i>.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"140-158"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142282796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flagella Phenotypic Variations of ST34 Type <i>Salmonella</i> Typhimurium and Variants.","authors":"Xin Wu, Fanan Suksawat, Patchara Phuektes, Supatcharee Siriwong, Hongmei Liu, Wengui Li, Sunpetch Angkititrakul","doi":"10.1089/fpd.2023.0091","DOIUrl":"10.1089/fpd.2023.0091","url":null,"abstract":"<p><p><i>Salmonella enterica</i> serovar Typhimurium and its variants are the most common serotypes of human salmonellosis cases. Serotyping <i>Salmonella</i> Typhimurium and its variants has always been challenging. Our previous work found that among 14 <i>Salmonella</i> Typhimurium and variant strains, some different antigenic formulas had 100% pulsed-field gel electrophoresis (PFGE) similarity. The 14 strains were sorted into 3 groups; in each group, the different antigenic formulas had the same PFGE patterns. This phenomenon suggested that different antigenic formula identification might originate from a common ancestor subtyped by PFGE. To assess whether the serotyping method on <i>Salmonella</i> Typhimurium and variant strains reflected the genetic relationship, we improved the discrimination for the phylogenetic relationship among the 14 <i>Salmonella</i> Typhimurium and variant strains using Fourier-transform infrared spectroscopy (FTIR) and whole-genome multilocus sequence typing (wgMLST). We compared the wgMLST assay of 14 <i>Salmonella</i> Typhimurium and variant strains from this study with 50 public ST34 strain data of <i>Salmonella</i> Typhimurium and variant strains. We also compared flagella (H antigen)-related genes based on the whole genome of 14 strains and the other 293 ST34 public database for further understanding of this question. The phylogenetic results (PFGE) showed no regularity between the antigenic formulas and genotypes. The results of the higher discrimination power assays (FTIR and whole-genome multilocus sequence typing) also showed no regularity between the antigenic formulas and genotypes (or phenotypes). The 58 flagella encoding genes of different antigenic formulas were sorted into 13 patterns. However, a similar phenomenon was found: the same flagella encoding gene patterns could express different antigenic formulas. In conclusion, there is no consistency between the antigenic formulas and phylogenetic relationships among ST34 <i>Salmonella</i> Typhimurium and variant strains, even in flagella antigenic formula and flagella encoding genes.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"159-166"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140101405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Resistance Profiles of <i>Campylobacter</i> spp. Recovered from Chicken Farms in Two Districts of Bangladesh.","authors":"Mehedi Hasan, Sudipta Talukder, Amit Kumar Mandal, Syeda Tanjina Tasmim, Sonia Parvin, Yamin Ali, Mahmudul Hasan Sikder, Thomas J Callaghan, Ricardo J Soares Magalhães, Taohidul Islam","doi":"10.1089/fpd.2023.0079","DOIUrl":"10.1089/fpd.2023.0079","url":null,"abstract":"<p><p>The rapid emergence of antimicrobial resistance (AMR) in <i>Campylobacter</i> has reinforced its status as a foodborne pathogen of significant public health concern. Resistant <i>Campylobacter</i> is typically transferred to humans via the consumption of contaminated animal products, particularly poultry. The genes associated with antimicrobial resistance in <i>Campylobacter</i> spp. are poorly understood. To address this knowledge gap, we conducted a prevalence survey of AMR <i>Campylobacter</i> across 84 chicken farms in two districts of Bangladesh. Pooled cloacal swabs were collected from chickens and underwent bacteriological testing for <i>Campylobacter</i> spp. with PCR confirmation. Antimicrobial susceptibility was tested against 14 antibiotics by disk diffusion method, and 12 resistance genes were screened in <i>Campylobacter</i>-positive isolates using multiplex PCR. A total of 34 (40.5%) farms were <i>Campylobacter</i>-positive of which 73.5% of isolates were resistant to at least 10 antibiotics. The antimicrobial susceptibility results indicate a high level of resistance against streptomycin (97.1%), clindamycin (97.1%), ampicillin (94.1%), tetracycline (94.1%), erythromycin (91.2%), ciprofloxacin (88.2%), nalidixic acid (85.3%), and imipenem (82.4%), and comparatively a low frequency of resistance to chloramphenicol (47.1%), ceftazidime (44.1%), and colistin (35.3%). Multidrug-resistant (MDR) and extensively drug-resistant <i>Campylobacter</i> were identified in 97.1%, and 50% of isolates, respectively. Ten resistance genes were identified including <i>bla</i>_TEM (in 97.1% of isolates), <i>strA</i>-<i>strB</i> (85.9%), <i>tetA</i> (70.6%), <i>tetB</i> (32.4%), <i>qnrS</i> (23.5%), <i>bla</i>_CTX-M-1 (20.6%), <i>qnrB</i> (20.6%), <i>bla</i>_SHV (8.8%), <i>aadB</i> (5.9%), and <i>qnrA</i> (2.9%). Our findings demonstrate that resistance to ampicillin, tetracycline, and ceftazidime in <i>Campylobacter</i> isolates was significantly (<i>p</i> ≤ 0.05) associated with the presence of <i>bla</i>_TEM, <i>tetA</i>, and <i>bla</i>_SHV genes, respectively. The high rates of AMR in <i>Campylobacter</i> isolates from our study are not surprising given the liberal use of antimicrobials and incomplete biosecurity provisions on farms. Of particular concern are resistance rates to those classes of antibiotics that should be reserved for human use (azithromycin, ciprofloxacin, and colistin). AMR was more prevalent in chicken farms that used multiple antibiotics, engaged in prophylactic treatment of the birds, and improperly disposed of antibiotic packages. The high prevalence of MDR in chicken-derived <i>Campylobacter</i> isolates from the different regions of our study reinforces the need for more prudent use of antimicrobial compounds in Bangladeshi chicken farms.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"118-130"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140335255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Resistance Characteristics of Fecal <i>Escherichia coli</i> and <i>Enterococcus</i> Species in U.S. Goats: 2019 National Animal Health Monitoring System Enteric Study.","authors":"Catherine A Gensler, Stephanie C Hempstead, Shivaramu Keelara, Paula J Fedorka-Cray, Natalie J Urie, Alyson M Wiedenheft, Keira Stuart, Katherine L Marshall, Megan E Jacob","doi":"10.1089/fpd.2023.0089","DOIUrl":"10.1089/fpd.2023.0089","url":null,"abstract":"<p><p><i>Escherichia coli</i> and <i>Enterococcus</i> species are normal bacteria of the gastrointestinal tract and serve as indicator organisms for the epidemiology and emergence of antimicrobial resistance in their hosts and the environment. Some <i>E. coli</i> serovars, including <i>E. coli</i> O157:H7, are important human pathogens, although reservoir species such as goats remain asymptomatic. We describe the prevalence and antimicrobial resistance of generic <i>E. coli</i>, <i>E. coli</i> O157:H7, and <i>Enterococcus</i> species collected from a national surveillance study of goat feces as part of the National Animal Health Monitoring System (NAHMS) Goat 2019 study. Fecal samples were collected from 4918 goats on 332 operations across the United States. Expectedly, a high prevalence of <i>E. coli</i> (98.7%, 4850/4915) and <i>Enterococcus</i> species (94.8%, 4662/4918) was found. <i>E. coli</i> O157:H7 prevalence was low (0.2%; 10/4918). <i>E. coli</i> isolates, up to three per operation, were evaluated for antimicrobial susceptibility and 84.7% (571/674) were pansusceptible. Multidrug resistance (MDR; ≥3 classes) was uncommon among <i>E. coli</i>, occurring in 8.2% of isolates (55/674). Resistance toward seven antimicrobial classes was observed in a single isolate. Resistance to tetracycline alone (13.6%, 92/674) or to tetracycline, streptomycin, and sulfisoxazole (7.0% 47/674) was the most common pattern. All <i>E. coli</i> O157:H7 isolates were pansusceptible. <i>Enterococcus</i> isolates, up to four per operation, were prioritized by public health importance, including <i>Enterococcus faecium</i> and <i>Enterococcus faecalis</i> and evaluated. Resistance to lincomycin (93.8%, 1232/1313) was most common, with MDR detected in 29.5% (388/1313) of isolates. The combination of ciprofloxacin, lincomycin, and quinupristin resistance (27.1%, 105/388) was the most common pattern detected. Distribution and characteristics of antimicrobial resistance in <i>E. coli</i> and <i>Enterococcus</i> in the U.S. goat population from this study can inform stewardship considerations and public health efforts surrounding goats and their products.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"97-108"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140174097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Prevalence of <i>Brucella</i> spp. in Dairy Products in Iran: A Systematic Review and Meta-Analysis.","authors":"Narges Shahbazpour, Elahesadat Hosseini, Seyed Ehsan Beladian Behbahan, Fatemeh Esfarjani, Seyed Amir Mohamad Mortazavian, Abdol-Samad Abedi, Slim Smaoui","doi":"10.1089/fpd.2024.0093","DOIUrl":"10.1089/fpd.2024.0093","url":null,"abstract":"<p><p>Brucellosis, a significant zoonotic disease, threatens food safety substantially, particularly in developing nations such as the Middle East. This study aimed to comprehensively assess the prevalence of <i>Brucella</i> spp. in Iranian milk and dairy products through a systematic review and meta-analysis. A thorough search of international and domestic databases from January 2008 to October 2023 identified 38 relevant studies encompassing 11,130 samples for meta-analysis. The overall prevalence of <i>Brucella</i> spp. in Iranian dairy products was 22% (95% CI: 16-28%). The highest and lowest overall prevalence of <i>Brucella</i> spp. in milk were found in raw goat milk 27% (95% CI: 11-42%) and raw camel milk 15% (95% CI: -0.42 to 72%), respectively. The overall prevalence of traditional cheese, ice cream, and cream is estimated to be 9% (95% CI: -16 to 35%), 2% (95% CI: -2.78 to 2.82%), and 9% (95% CI: -0.94 to 1.12%). Geographical disparities were evident, with Zanjan province reporting the highest contamination rate, 53%, while Razavi Khorasan province had the lowest, 1%. However, the prevalence of <i>Brucella</i> spp. in Iranian dairy products has fluctuated over time, with a significant association between the study year and sample size. Comprehensive planning, robust policy implementation, and rigorous monitoring are imperative to mitigate and ultimately eliminate <i>Brucella</i> contamination in dairy products effectively. Further research is essential to refine prevalence estimates and develop targeted prevention strategies to safeguard public health.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"71-85"},"PeriodicalIF":1.9,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}