Foodborne pathogens and disease最新文献

{"title":"Rotavirus Infections: A 2-Year Comprehensive Review in Admitted Pediastric Patients Amid Conflicting National Policies.","authors":"Yusuf Deniz, Burak Demircioğlu, İrem Kuter-Emeklioğlu, Sadrettin Ekmen, Eylem Sevınc, Erkan Dogan","doi":"10.1089/fpd.2024.0019","DOIUrl":"https://doi.org/10.1089/fpd.2024.0019","url":null,"abstract":"<p><p>Highlighting rotavirus (RV) as a significant food and waterborne pathogen, particularly affecting infants and children, causing serious gastrointestinal infections and dehydration, is important. It should be noted that there are significant debates regarding the effectiveness and outcomes of RV vaccination. In contrast to Turkey's nonmandatory vaccination policy, many developed countries implement mandatory vaccination policies, raising questions about their impact on disease prevalence and healthcare expenditures. Our study aims to comprehensively understand RV infections in Turkey and compare them with countries that have mandatory vaccination policies. We found similar, and even better, hospitalization rates, length of hospital stays, and laboratory parameters demonstrating the effectiveness of Turkey's independent vaccination approach. These findings contribute valuable insights to global vaccination strategies and disease control.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142055334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiomics-Based Biocargo Components Analysis in <i>Enterococcus faecium</i> Membrane Vesicles.","authors":"Meiying Luo, Yuanyuan Zhu, Xiaofang Zhang, Junhang Sun, Xin Feng, Huihua Zhang, Qien Qi","doi":"10.1089/fpd.2024.0031","DOIUrl":"https://doi.org/10.1089/fpd.2024.0031","url":null,"abstract":"<p><p><i>Enterococcus</i> spp. have been shown to have gastrointestinal tract protective functions; our recent results suggest that membrane vesicles (MVs) play an important role in the gastric protection of <i>Enterococcus faecium</i> (<i>E. faecium</i>). The specific function is determined by molecular compositions of MVs. To resolve biocargo components in <i>E. faecium</i> MVs (<i>Efm</i>MVs), MVs were isolated from <i>E. faecium</i> culture. Transcriptomics, label-free quantitative proteomics, and untargeted metabolomics were performed to obtain information about the complexity of ribonucleic acids (RNAs), proteins, and metabolites biocargo they carry, respectively. RNA-sequencing identified a total of 2122 transcripts. The top 20 transcripts accounted for 27.63% of total counts, which, including enzymes, participate in glycolysis, ribosomal proteins, DNA-directed RNA polymerases, protein-synthesizing relative enzymes, molecules associated with protein post-translational processing and transport, and peptidoglycan lyases. Label-free quantitative proteomics analysis identified a total of 711 proteins. The top 20 proteins accounted for 48.02% of all identified proteins, which including ribosomal proteins, enzymes participate in glycolysis, DNA-directed RNA polymerases, protein-synthesizing relative enzymes, peptidoglycan lyases, and autolysin. Untargeted metabolomics analysis identified a total of 519 metabolites. The top 20 metabolites accounted for 79.55% of all identified metabolites, which included amino acids, substrates, or products in the metabolism of amino acids, natural organic acids, products in the metabolism of organic acids, ketone compounds, and two other compounds. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses indicated that the identified biocargo components enriched in metabolism, genetic, and environmental information processing. Overall, we hope that the current exploration of multiple \"-omics\" analyses of this <i>Efm</i>MVs will provide useful information and further groundwork for future studies on <i>E. faecium</i> application.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contamination Status and Serotypes Distribution of <i>Salmonella</i> in Food in Yantai City, China: A 14-Year Continuous Monitoring Study.","authors":"Fengguang Dong, Guiqiang Wang, Xueying Feng, Chunbo Gong, Wenjuan Liu, Songsong Wang, Yiyi Zhang, Yapeng Huo, Youxia Chen, Hongtao Wang","doi":"10.1089/fpd.2024.0055","DOIUrl":"https://doi.org/10.1089/fpd.2024.0055","url":null,"abstract":"<p><p><i>Salmonella</i> is a foodborne zoonotic pathogen that threatens food safety and public health. However, few people have conducted long-term and systematic studies on <i>Salmonella</i> contamination in food in Yantai City. In order to investigate the situation of <i>Salmonella</i> contamination in food and improve the ability of early warning and control of foodborne diseases, a total of 3420 samples from 20 categories were collected from 13 monitoring points in Yantai City, from 2010 to 2023. The difference in detection rate and bacterial strain of different monitoring points, different types, and different sources of samples was compared. Of the 3420 samples, 80 were positive with a detection rate of 2.34%. <i>Salmonella</i> detection rates were significantly different for samples collected at different monitoring sites. <i>Salmonella</i> was detected only in meat and meat products and catering food, and none of the other types were detected. The detection rate of <i>Salmonella</i> was higher in raw animal meat and raw poultry. Samples collected at the market stage had the highest detection rate (5.81%), and there was a significant difference in detection rate between samples from different sources (χ² = 36.93, <i>p</i> < 0.05). Eighty-one strains of <i>Salmonella</i> were detected out of 3420 samples (2 different strains were detected in 1 positive sample). The serological test identified 8 groups and 27 serotypes. The dominant serum groups were group B 30.86% (25/81), group E1 23.46% (19/81), and group D 16.05% (13/81). The main dominant serotypes were <i>Salmonella</i> <i>give</i> 17.28% (14/81), <i>Salmonella</i> <i>enteritidis</i> 16.05% (13/81), and <i>Salmonella</i> <i>derby</i> 13.58% (11/81). Meat and meat products and catering food were the main food products contaminated with <i>Salmonella</i>. The resulting secondary contamination is the hidden threat of foodborne diseases and should be given sufficient attention.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141909835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Duplex PCR Assay for Rapid Detection of <i>Klebsiella pneumoniae</i> and <i>Chryseobacterium</i> in Large Yellow Croaker Fish.","authors":"Gaowei Hu, Longfei Yin, Xi Luo, Yingjie Miao, Jianyun Yu","doi":"10.1089/fpd.2023.0149","DOIUrl":"10.1089/fpd.2023.0149","url":null,"abstract":"<p><p>Both <i>Klebsiella pneumoniae</i> and <i>Chryseobacterium</i> cause an increasing number of diseases in fish, resulting in great economic losses in aquaculture. In addition, the disease infected with <i>Klebsiella pneumoniae</i> or <i>Chryseobacterium</i> exhibited the similar clinical symptoms in aquatic animals. However, there is no effective means for the simultaneous detection of co-infection and discrimination them for these two pathogens. Here, we developed a duplex polymerase chain reaction (PCR) method based on the outer membrane protein A (<i>ompA</i>) gene of <i>Klebsiella pneumoniae</i> and <i>Chryseobacterium</i>. The specificity and validity of the designed primers were confirmed experimentally using simplex PCR. The expected amplicons for <i>Klebsiella pneumoniae</i> and <i>Chryseobacterium</i> had a size of 663 and 1404 bp, respectively. The optimal condition for duplex PCR were determined to encompass a primer concentration of 0.5 μM and annealing temperature of 57°C. This method was analytical specific with no amplification being observed from the genomic DNA of <i>Escherichia coli</i>, <i>Vibrio harveyi</i>, <i>Pseudomonas plecoglossicida</i>, <i>Aeromonas hydrophila</i> and <i>Acinetobacter johnsonii</i>. The limit of detection was estimated to be 20 fg of genomic DNA for <i>Chryseobacterium</i> and 200 fg for <i>Klebsiella pneumoniae</i>, or 100 colony-forming units (CFU) of bacterial cells in both cases. The duplex PCR was capable of simultaneously amplifying target fragments from genomic DNA extracted from the bacteria and fish liver. For practical validation of the method, 20 diseased fish were collected from farms, among which 4 samples were PCR-positive for <i>Klebsiella pneumoniae</i> and <i>Chryseobacterium</i>. The duplex PCR method developed here is time-saving, specific, convenient, and may prove to be an invaluable tool for molecular detection and epidemiological investigation of <i>Klebsiella pneumoniae</i> and <i>Chryseobacterium</i> in the field of aquaculture.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140850108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Biological Characteristics of a Novel Phage and Its Application to Control <i>Vibrio Parahaemolyticus</i> in Shellfish Meat.","authors":"Wenting Liu, Yiming Wu, Huajuan Wang, Hongxun Wang, Min Zhou","doi":"10.1089/fpd.2023.0133","DOIUrl":"10.1089/fpd.2023.0133","url":null,"abstract":"<p><p><i>Vibrio parahaemolyticus</i> is a common foodborne pathogenic bacterium. With the overuse of antibiotics, an increasing proportion of drug-resistant strains are emerging, which puts enormous pressure on public health. In this study, a <i>V. parahaemolyticus</i>-specific phage, VP41s3, was isolated. The head length, width, and tail length of the phage were 77.7 nm, 72.2 nm, and 17.5 nm, respectively. It remained active in the temperature range of 30-50°C and pH range of 4-11. The lytic curve of phage VP41s3 showed that the host bacteria did not grow until 11 h under phage treatment at MOI of 1000, indicating that the phage had good bacteriostatic ability. When it was added to shellfish contaminated with <i>V. parahaemolyticus</i> (15°C, 48 h), the number of bacteria in the experimental group was 2.11 log₁₀ CFU/mL lower than that in the control group at 24 h. Furthermore, genomic characterization and phylogenetic analysis indicated that phage VP41s3 was a new member of the <i>Podoviridae</i> family. The genome contained 50 open reading frames (ORFs), in which the ORF19 (thymidine kinase) was an enzyme involved in the pyrimidine salvage pathway, which might lead to the accelerated DNA synthesis efficiency after phage entered into host cells. This study not only contributed to the improvement of phage database and the development of beneficial phage resources but also revealed the potential application of phage VP41s3 in food hygiene and safety.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140957168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of <i>Salmonella</i> Enteritidis into a Viable but Nonculturable State by Cinnamaldehyde and Its Resuscitation.","authors":"Ziying Lu, Tong Xing, Zepeng Zhao, Zefeng Li, Dongping Hou, Zhuolin Ma, Siyi Chen, Yuheng Yang, Shaoting Li, Hongmei Zhang","doi":"10.1089/fpd.2023.0186","DOIUrl":"10.1089/fpd.2023.0186","url":null,"abstract":"<p><p>Trans-cinnamaldehyde (TC), a typical plant-derived compound, has been widely used in the control of foodborne pathogen contamination. Nevertheless, the risk associated with the occurrence of viable but nonculturable (VBNC) bacteria induced by TC remains unclear. The results of this study showed that <i>Salmonella</i> Enteritidis (<i>S.</i> Enteritidis) entered the VBNC state after being induced by TC at a minimum inhibitory concentration of 312.5 μg/mL and survived for at least 22 days under TC treatment. Enhanced resistance was found against heat treatment (75°C, 30 s), antibiotics (i.e., ampicillin, ceftriaxone sodium, chloramphenicol), and hydrogen peroxide (3%) in VBNC <i>S.</i> Enteritidis. A synergistic effect against VBNC <i>S.</i> Enteritidis occurred when TC was combined with acid treatment, including lactic acid and acetic acid (pH = 3.5). VBNC and resuscitated <i>S</i>. Enteritidis by sodium pyruvate treatment (100 mM) were found to retain the infectious ability to Caco-2 cells. Relative expression levels of the stress-related genes <i>relA</i>, <i>spoT</i>, <i>ppx</i>, <i>lon</i>, <i>katG</i>, <i>sodA</i>, <i>dnaK</i>, and <i>grpE</i> were upregulated in VBNC <i>S</i>. Enteritidis. Accumulation of reactive oxygen species (ROS) and protein aggregates was observed in VBNC cells. Besides, the resuscitation of VBNC cells was accompanied with clearance of ROS and protein aggregates. In summary, this study presents a comprehensive characterization of stress tolerance and resuscitation of VBNC <i>S</i>. Enteritidis induced by cinnamaldehyde, and the results provide useful information for the development of effective control strategy against VBNC pathogenic bacteria in food production.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140853382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomics Characterization of Colistin Resistant <i>Escherichia coli</i> from Chicken Meat-the First Report in the United Arab Emirates.","authors":"Ihab Habib, Mohamed-Yousif Ibrahim Mohamed, Mohammed Elbediwi, Akela Ghazawi, Mushtaq Khan, Afra Abdalla, Glindya Bhagya Lakshmi","doi":"10.1089/fpd.2024.0021","DOIUrl":"10.1089/fpd.2024.0021","url":null,"abstract":"<p><p>Plasmid-mediated colistin resistance is an emerging One Health challenge at the human-food-environment interface. In this study, 12 colistin-resistant <i>Escherichia coli</i> carrying <i>mcr-1.1</i> gene were characterized using whole-genome sequencing. This is the first report from locally produced chicken meat in the United Arab Emirates. The characterized isolates harbored virulence-associated factors ranging from 4 to 17 genes per isolate. The multilocus sequence type 1011 was identified in 5 (41.6%) isolates. Six (50.0%) of the isolates harbored <i>bla</i>_CTX-M-55. All of the <i>E. coli</i> isolates contained Incl2 plasmids. This study highlights for the first time chicken meat as a potential reservoir of <i>mcr-1.1</i> carrying <i>E. coli</i> in the UAE. This study has implications for food safety and underscores the need for comprehensive surveillance strategies to monitor the spread of colistin resistance. Results presented in this short communication address knowledge gaps on the epidemiology of plasmid-mediated colistin resistance in the Middle East food production chain.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141157214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteriological Survey of Insect Products in Japan.","authors":"Yukako Simojima, Tamako Ishikawa, Erika Noguchi, Reina Araki, Kai Gomyo, Itsuki Miyajima, Yuka Akita, Yui Ohara, Ryuga Nakagawa, Yumiko Okada, Yukio Morita","doi":"10.1089/fpd.2024.0004","DOIUrl":"10.1089/fpd.2024.0004","url":null,"abstract":"<p><p>A microbiological study was conducted on 41 insect product samples (29 raw frozen [21 domestic and 8 imported], 10 powdered, and 2 processed), which were commercially available in Japan. The total aerobic count for raw frozen insects was 5.61 log cfu/g (range: 2.52-8.40), whereas the powdered insect count was 2.89 log cfu/g (range: 1.00-4.57). The bacterial count was significantly higher in raw frozen insects (<i>p</i> < 0.05). The coliform count for the raw frozen insects ranged from <1 to 6.90 log cfu/g, and that for the powdered insects ranged from <1 to 1.00 log cfu/g. The number of samples with values above the detection limit was significantly higher in raw frozen insects (<i>p</i> < 0.05). The detection frequencies of aerobic spores (<1-4.63 log cfu/g), anaerobic spores (<0-4.40 log cfu/g), and <i>Bacillus cereus</i> (<1.7-3.83 log cfu/g) showed no sample type-related significant difference. <i>Listeria</i> spp. was isolated from four samples of raw frozen insects, one of which was <i>Listeria monocytogenes</i>. We did not detect any of the following: <i>Salmonella</i> spp., Shiga toxin-producing <i>E. coli</i> (STEC), <i>Campylobacter jejuni/coli</i>, or pathogenic <i>Yersinia</i>. We isolated insect products retailed in Japan harboring food poisoning bacteria, including <i>L. monocytogenes</i> and <i>B. cereus</i>. In particular, raw frozen products displayed high levels of hygienic indicator bacteria.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140854927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a New Multispecies Protein A-Based ELISA for Serodiagnosis of Hepatitis E Infection with Validation in Domestic Swine.","authors":"Maria Gabriela Oliveira da Paz, Matheus Filgueira Bezerra, Andrei Félix Mendes, Adalucia da Silva, Gustavo Barbosa de Lima, Laura Helena Vega Gonzales Gil, Lindomar José Pena, George Tadeu Nunes Diniz, Jan Felix Drexler, Reinaldo Barreto Oriá, Edmilson Ferreira de Oliveira-Filho, Christian Robson de Souza Reis","doi":"10.1089/fpd.2023.0179","DOIUrl":"10.1089/fpd.2023.0179","url":null,"abstract":"<p><p>Hepatitis E virus (HEV) infects roughly 20 million people worldwide, causing self-limiting acute hepatic disease that can evolve into a chronic course. HEV-3, HEV-4, and HEV-7 genotypes are zoonotic and transmitted to humans by consuming raw or undercooked meat. Here, we developed an indirect ELISA based on the recombinant HEV-3 capsid and performed a seroprevalence study on domestic swine in northeastern Brazil. Our in-house ELISA was initially validated using a subset of 79 sera characterized by concordant results for two distinct commercial ELISA kits. Our ELISA exhibited excellent sensitivity (94%) and specificity (100%), with an area under the curve of 0.99 Further testing, including 212 swine sera, revealed a seroprevalence of 57.5% (95% confidence interval, 50.6-64.3%). Our findings indicate that the novel ELISA test could accurately detect specific anti-HEV antibodies in domestic pigs and should be further validated in humans and other mammals.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140863486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abundance of Colistin-Resistance Genes in Retail Meats in Vietnam.","authors":"Yen Hai Le, Kanoko Ikawa, Hoa Thi Thanh Hoang, Hatsue Isomura, Diep Thi Khong, Thang Nam Nguyen, Tram Anh Que, Dung Tien Pham, Kaori Tanaka, Yoshimasa Yamamoto","doi":"10.1089/fpd.2023.0162","DOIUrl":"10.1089/fpd.2023.0162","url":null,"abstract":"<p><p>The degree of contamination of retail meat with colistin-resistant bacteria and its potential contribution to dissemination within communities remains to be determined. Thus, we aimed to elucidate the contamination status of colistin-resistance genes, indicative of colistin-resistant bacteria, in retail meats in Vietnam. In total, 46 chicken and 49 pork meats from stores in Vietnam and Japan were examined. Multiplex real-time polymerase chain reaction with TaqMan probes was performed for detecting <i>mcr-1</i>, <i>mcr-3</i>, and <i>Escherichia coli</i> <i>16S rRNA</i>. Colistin-resistant bacteria in meats were isolated using selective media. The minimum inhibitory concentrations of colistin were determined using the broth microdilution method. The results showed that 70.7% of chicken meats in Vietnam were contaminated with both <i>mcr-1</i> and <i>mcr-3</i>. Meanwhile, <i>mcr-1</i> and <i>mcr-3</i> were detected in 15.9% and 40.9% of pork meat, respectively. Only <i>mcr-3</i> was detected in 40% of chicken in Japan. In addition, <i>mcr-1</i>-harboring <i>E. coli</i> and <i>mcr-3</i>-harboring <i>Aeromonas</i> were isolated from chicken meats in Vietnam. Some of these isolates showed colistin resistance. These results showed that most retail meats were highly contaminated with colistin-resistance genes. Notably, our results suggest that <i>mcr-3</i> is more prevalent in the contaminated samples compared with <i>mcr-1</i>.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140854926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}