Foodborne pathogens and disease最新文献

{"title":"Antibacterial Activity and Mechanism of Lauric Acid Against <i>Staphylococcus aureus</i> and Its Application in Infectious Cooked Chicken.","authors":"Liu Qingyan, Shi Susu, Liu Shuanglin, Xian Youhua, Yan Haiyang, Yuan Yuan","doi":"10.1089/fpd.2024.0063","DOIUrl":"https://doi.org/10.1089/fpd.2024.0063","url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> contamination and prevention has always been a major concern for food industry. This work investigated the antibacterial activity and mechanisms of lauric acid (LA) against <i>S. aureus</i>. Results revealed 156 μg/mL was the minimum inhibitory concentration (MIC) for LA and it retarded growth rate of <i>S. aureus</i>. The inhibitory effect was enhanced with LA concentration. After being treated with 2 MIC LA for 24 h, the number of <i>S. aureus</i> decreased by 3.56 log colony-forming unit (CFU)/mL. Scanning electron microscopy profiling revealed that LA resulted in altered morphology of <i>S. aureus</i> cells. In addition, propidium iodide staining of flow cytometry suggested that LA treatment disrupted the cell membrane integrity. Changes in 8-anilino-1-naphthalenesulfonic acid fluorescence indicated a depolarization change in cell membrane fluidity. For practical applications, LA also displayed an antimicrobial potential in cooked chicken food model system, with 1.25-5 g/L of LA prolonging shelf life by 2 days at 4°C. Moreover, it had no adverse effect on pH values, color in cooked chicken meat, and even reduced lipid oxidation. To sum up, LA has great antimicrobial properties and is a candidate preservative for cooked meat food.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142125264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The <i>ldh</i> Gene Plays a Crucial Role in Mediating the Pathogen Control of <i>Lactiplantibacillus plantarum</i> AR113.","authors":"Xue Li, Guangqiang Wang, Jun Wang, Xin Song, Zhiqiang Xiong, Yongjun Xia, Lianzhong Ai","doi":"10.1089/fpd.2024.0028","DOIUrl":"10.1089/fpd.2024.0028","url":null,"abstract":"<p><p>Effectively managing foodborne pathogens is imperative in food processing, where probiotics play a crucial role in pathogen control. This study focuses on the <i>Lactiplantibacillus plantarum</i> AR113 and its gene knockout strains, exploring their antimicrobial properties against <i>Escherichia coli</i> O157:H7 and <i>Staphylococcus aureus</i>. Antimicrobial assays revealed that the inhibitory effect of AR113 increases with its growth and the potential bacteriostatic substance is acidic. AR113Δ<i>ldh,</i> surpassed AR113Δ<i>0273&2024</i>, exhibited a complete absence of bacteriostatic properties, which indicates that lactic acid is more essential than acetic acid in the bacteriostatic effect of AR113. However, the exogenous acid validation test affirmed the equivalent superior bacteriostatic effect of lactic acid and acetic acid. Notably, AR113 has high lactate production and deletion of the <i>ldh</i> gene not only lacks lactate production but also affects acetic production. This underscores the <i>ldh</i> gene's pivotal role in the antimicrobial activity of AR113. In addition, among all the selected knockout strains, AR113Δ<i>tagO</i> and Δ<i>ccpA</i> also had lower antimicrobial effects, suggesting the importance of <i>tagO</i> and <i>ccpA</i> genes of AR113 in pathogen control. This study contributes insights into the antimicrobial potential of AR113 and stands as the pioneering effort to use knockout strains for comprehensive bacteriostatic investigations.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"578-585"},"PeriodicalIF":1.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141497599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence, Antimicrobial Resistance, and Diversity of <i>Campylobacter</i> Isolated from U.S. Goat Feces: 2019 NAHMS Survey.","authors":"Catherine A Gensler, Stephanie C Hempstead, Shivaramu Keelara, Paula J Fedorka-Cray, Natalie J Urie, Alyson M Wiedenheft, Katherine L Marshall, Matthew Branan, Kiera Stuart, Kristina Lantz, Megan E Jacob","doi":"10.1089/fpd.2023.0080","DOIUrl":"10.1089/fpd.2023.0080","url":null,"abstract":"<p><p>Goats are often asymptomatic carriers of <i>Campylobacter</i>, including the foodborne pathogen <i>Campylobacter jejuni.</i> Infections can have significant and economically detrimental health outcomes in both humans and animals. The primary objective of this study was to estimate the prevalence of <i>Campylobacter</i> in U.S. goat herds. <i>Campylobacter</i> species were isolated from 106 of 3,959 individual animals and from 42 of 277 goat operations that participated in fecal sample collection as part of the National Animal Health Monitoring System Goat 2019 study. Weighted animal-level prevalence was 2.3% (SE = 0.5%) and operation prevalence was 13.0% (SE = 3.2%). Animal-level prevalence ranged widely from 0 to 70.0%, however, 52.4% of positive operations (22/42) had only a single isolate. <i>C. jejuni</i> was the most frequently isolated species (68.9%; 73/106), followed by <i>C. coli</i> (29.3%, 31/106). A total of 46.2% (36/78) of viable isolates were pan-susceptible to 8 antimicrobials. Resistance to tetracycline (TET) was observed in 44.9% (35/78) of isolates, while 12.8% (10/78) were resistant to ciprofloxacin (CIP) and nalidixic acid (NAL). Among all isolates, a single resistance profile CIP-NAL-TET was observed in 3.8% (3/78) of isolates. A total of 35 unique sequence types (STs) were identified, 11 of which are potentially new. Multiple <i>C. jejuni</i> STs were observed in 48.1% (13/27) of positive operations. Goats with access to surface water, operations reporting antibiotics in the feed or water (excluding ionophores and coccidiostats), and operations reporting abortions and without postabortion management tasks had significantly greater odds of being <i>Campylobacter</i> positive. This snapshot of the U.S. goat population enriches the limited pool of knowledge on <i>Campylobacter</i> species presence in U.S. goats.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"546-559"},"PeriodicalIF":1.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141491482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and Characteristics of <i>Salmonella</i> from Tibetan Pigs in Tibet, China.","authors":"Gang Wang, Xilong Kang, Shuangjuan Wang, Chuang Meng, Dan Gu, Li Song, Xinan Jiao, Zhiming Pan","doi":"10.1089/fpd.2024.0069","DOIUrl":"10.1089/fpd.2024.0069","url":null,"abstract":"<p><p>This study aimed to understand the epidemiological characteristics of <i>Salmonella</i> in Tibetan pigs. We isolated, identified, and examined via antimicrobial susceptibility testing on <i>Salmonella</i> from Tibetan pigs breeder farms and slaughterhouses in Tibet, China. A genetic evolutionary tree was constructed on the basis of whole genome sequencing (WGS). A total of 81 <i>Salmonella</i> isolates were isolated from 987 samples. The main serovars were <i>Salmonella</i> Typhimurium and <i>Salmonella</i> London in Tibetan pigs. The isolated <i>Salmonella</i> Typhimurium isolates subjected to antimicrobial susceptibility testing showed varying degrees of resistance to β-lactams, aminoglycosides, fluoroquinolones, sulfonamides, tetracyclines, and amphenicols. WGS analysis was performed on 20 <i>Salmonella</i> Typhimurium isolates in Tibet (<i>n</i> = 10), Jiangsu (<i>n</i> = 10), and 205 genome sequences downloaded from the Enterobase database to reveal their epidemiological and genetic characteristics. They were divided into two clusters based on core genome single-nucleotide polymorphisms: Cluster A with 112 isolates from Tibet and other regions in China and Cluster B with 113 isolates from Jiangsu and other regions. The isolates in Cluster A were further divided into two subclusters: A-1 with 40 isolates including Tibet and A-2 with 72 isolates from other regions. Virulence factors analysis revealed that all isolates from Tibet carried <i>adeG</i>, but this observation was not as common in <i>Salmonella</i> isolates from Jiangsu and other regions of China. Antibiotic resistance genes (ARGs) analysis showed that all isolates from Tibet carried <i>bla_TEM-55</i> and <i>rmtB</i>, which were absent in <i>Salmonella</i> isolates from Jiangsu and other regions of China. Genetic characteristic analysis and biofilm determination indicated that the biofilm formation capabilities of the isolates from Tibet were stronger than those of the isolates from Jiangsu and other regions of China. Our research revealed the epidemic patterns and genomic characteristics of <i>Salmonella</i> in Tibetan pigs and provided theoretical guidance for the prevention and control of local salmonellosis.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"560-569"},"PeriodicalIF":1.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141598968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of <i>Listeria monocytogenes</i> in Milk and Dairy Product Supply Chains: A Global Systematic Review and Meta-analysis.","authors":"Xin Li, Jingying Zheng, Wei Zhao, Yafang Wu","doi":"10.1089/fpd.2024.0029","DOIUrl":"10.1089/fpd.2024.0029","url":null,"abstract":"<p><p><i>Listeria monocytogenes</i>, one of the main foodborne pathogens, is commonly found in milk and dairy products. This study aimed to estimate the presence of <i>L. monocytogenes</i> in milk and dairy product supply chains using a meta-analysis based on PubMed, Embase, Web of Science, and Scopus databases. A total of 173 studies were included in this meta-analysis. The pooled prevalence in the supply chain environment was 8.69% (95% confidence interval [CI]: 5.30%-12.78%), which was higher than that in dairy products (4.60%, 95% CI: 1.72%-8.60%) and milk products (2.93%, 95% CI: 2.14%-3.82%). Subgroup analysis showed that <i>L. monocytogenes</i> prevalence in raw milk (3.44%, 95% CI: 2.61%-4.28%) was significantly higher than in pasteurized milk (0.60%, 95% CI: 0.00%-2.06%). The highest prevalence of <i>L. monocytogenes</i> in milk and dairy products was observed in North America (5.27%, 95% CI: 2.19%-8.35%) and South America (13.54%, 95% CI: 3.71%-23.37%). In addition, studies using culture and molecular methods (5.17%, 95% CI: 2.29%-8.06%) had higher prevalence than other detection methods. Serogroup 1/2a and 3a (45.34%, 95% CI: 28.74%-62.37%), serogroup 1/2b and 3b (14.23%, 95% CI: 6.05%-24.24%), and serogroup 4b/4e (13.71%, 95% CI: 6.18%-22.83%) were dominant in these studies. The results of this study provide a better understanding of the prevalence of <i>L. monocytogenes</i> in milk and dairy product supply chains and suggest a potential foodborne pathogen burden.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"526-535"},"PeriodicalIF":1.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141431758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome Mining of Novel Targets and Construction of Ladder-shaped melting temperature isothermal amplification Assays for the Identification of <i>Cronobacter sakazakii</i> and <i>Cronobacter malonaticus</i>.","authors":"Da Ma, Ling Deng, Liangpi Huang, Aoxiang Peng, Wanglai Bi, Rui Li","doi":"10.1089/fpd.2024.0001","DOIUrl":"10.1089/fpd.2024.0001","url":null,"abstract":"<p><p><i>Cronobacter</i> species are potential pathogens that can contaminate powdered infant formula. <i>C. sakazakii</i> and <i>C. malonaticus</i> are the most common species of <i>Cronobacter</i> associated with infections. This study mined new molecular targets for the detection of <i>C. sakazakii</i> and <i>C. malonaticus</i> by using comparative genome approaches. Specific target genes <i>mngB</i> and <i>ompR</i> were obtained and used to detect <i>C. sakazakii</i> and <i>C. malonaticus,</i> respectively. A novel detection method, termed ladder-shape melting temperature isothermal amplification (LMTIA), was developed and evaluated. The detection limit for pure <i>C. sakazakii</i> DNA was 1 pg per reaction and 1 fg per reaction for <i>C. malonaticus</i>. The <i>C. sakazakii</i>, <i>C. malonaticus</i>, and the reference stains were all correctly identified. The amplicons can be successfully visualized and identified by naked eyes when hydroxy naphthol blue dye (HNB dye) was used in the reaction. Therefore, the LMTIA assays developed in this study showed potential application for microorganism identification and detection.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"586-592"},"PeriodicalIF":1.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141534111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rosalind Franklin Society Proudly Announces the 2023 Award Recipient for <i>Foodborne Pathogens and Disease</i>.","authors":"Megan E Jacob","doi":"10.1089/fpd.2024.79803.rfs2023","DOIUrl":"https://doi.org/10.1089/fpd.2024.79803.rfs2023","url":null,"abstract":"","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":"21 9","pages":"525"},"PeriodicalIF":1.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Into the Jungle of Biological Agents of Foodborne Diseases: Time to Put Some Order for the French Risk Manager.","authors":"Frédérique Audiat-Perrin, Laurent Guillier, Jean-Christophe Augustin, Gilles Bornert, Michel Federighi, Michel Gautier, Nathalie Jourdan-da Silva, Régis Pouillot, Myriam Merad, Moez Sanaa, Pauline Kooh","doi":"10.1089/fpd.2023.0105","DOIUrl":"10.1089/fpd.2023.0105","url":null,"abstract":"<p><p>Consumers can be exposed to many foodborne biological hazards that cause diseases with varying outcomes and incidence and, therefore, represent different levels of public health burden. To help the French risk managers to rank these hazards and to prioritize food safety actions, we have developed a three-step approach. The first step was to develop a list of foodborne hazards of health concern in mainland France. From an initial list of 335 human pathogenic biological agents, the final list of \"retained hazards\" consists of 24 hazards, including 12 bacteria (including bacterial toxins and metabolites), 3 viruses and 9 parasites. The second step was to collect data to estimate the disease burden (incidence, Disability Adjusted Life Years) associated with these hazards through food during two time periods: 2008-2013 and 2014-2019. The ranks of the different hazards changed slightly according to the considered period. The third step was the ranking of hazards according to a multicriteria decision support model using the ELECTRE III method. Three ranking criteria were used, where two reflect the severity of the effects (Years of life lost and Years lost due to disability) and one reflects the likelihood (incidence) of the disease. The multicriteria decision analysis approach takes into account the preferences of the risk managers through different sets of weights and the uncertainties associated with the data. The method and the data collected allowed to estimate the health burden of foodborne biological hazards in mainland France and to define a prioritization list for the health authorities.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"536-545"},"PeriodicalIF":1.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141534112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial Effect of <i>Euryale ferox</i> Seed Shell Polyphenol Extract on <i>Salmonella Typhimurium</i>.","authors":"Meihong Cai, Qiaoqiao Xu, Shili Zhao, Xianhan Zhou, Yuelin Cai, Xingle He","doi":"10.1089/fpd.2023.0185","DOIUrl":"10.1089/fpd.2023.0185","url":null,"abstract":"<p><p>This study aimed to evaluate the effects of <i>Euryale ferox</i> Seed Shell Polyphenol Extract (EFSSPE) on a foodborne pathogenic bacterium. EFSSPE showed antimicrobial activity toward <i>Salmonella</i> Typhimurium CICC 22956; the minimum inhibitory concentration of EFSSPE was 1.25 mg/mL, the inhibition curve also reflected the inhibitory effect of EFSSPE on the growth of <i>S</i>. Typhimurium. Detection of alkaline phosphatase outside the cell revealed that EFSSPE treatment damaged the cell wall integrity of <i>S.</i> Typhimurium. EFSSPE also altered the membrane integrity, thereby causing leaching of 260-nm-absorbing material (bacterial proteins and DNA). Moreover, the activities of succinate dehydrogenase and malate dehydrogenase were inhibited by EFSSPE. The hydrophobicity and clustering ability of cells were affected by EFSSPE. Scanning electron microscopy showed that EFSSPE treatment damaged the morphology of the tested bacteria. These results indicate that EFSSPE can destroy the cell wall integrity and alter the permeability of the cell membrane of <i>S.</i> Typhimurium.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"570-577"},"PeriodicalIF":1.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141491481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediction of <i>Listeria monocytogenes</i> Clonal Complexes from Multilocus Variable Number Tandem Repeat Analysis Patterns Using a Machine Learning Approach.","authors":"Nicholas Andrews, Natalia Unrath, Patrick Wall, James F Buckley, Séamus Fanning","doi":"10.1089/fpd.2023.0163","DOIUrl":"10.1089/fpd.2023.0163","url":null,"abstract":"<p><p>Multilocus variable number tandem repeat analysis (MLVA) is a molecular subtyping technique that remains useful for those without the resources to access whole genome sequencing for the tracking and tracing of bacterial contaminants. Unlike techniques such as multilocus sequence typing (MLST) and pulsed-field gel electrophoresis, MLVA did not emerge as a standardized subtyping method for <i>Listeria monocytogenes</i>, and as a result, there is no reference database of virulent or food-associated MLVA subtypes as there is for MLST-based clonal complexes (CCs). Having previously shown the close congruence of a 5-loci MLVA scheme with MLST, a predictive model was created using the XGBoost machine learning (ML) technique, which enabled the prediction of CCs from MLVA patterns with ∼85% (±4%) accuracy. As well as validating the model on existing data, a straightforward update protocol was simulated for if and when previously unseen subtypes might arise. This article illustrates how ML techniques can be applied with elementary coding skills to add value to previous-generation molecular subtyping data in-built food processing environments.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"593-599"},"PeriodicalIF":1.9,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141534113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}