Foodborne pathogens and disease最新文献

筛选
英文 中文
Acknowledgment of Reviewers 2024. 审稿人致谢
IF 1.9 2区 农林科学
Foodborne pathogens and disease Pub Date : 2025-01-01 DOI: 10.1089/fpd.2024.84560.revack
{"title":"Acknowledgment of Reviewers 2024.","authors":"","doi":"10.1089/fpd.2024.84560.revack","DOIUrl":"https://doi.org/10.1089/fpd.2024.84560.revack","url":null,"abstract":"","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":"22 1","pages":"66-69"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Contamination Status and Serotypes Distribution of Salmonella in Food in Yantai City, China: A 14-Year Continuous Monitoring Study. 中国烟台市食品中沙门氏菌的污染状况和血清型分布:为期 14 年的连续监测研究。
IF 1.9 2区 农林科学
Foodborne pathogens and disease Pub Date : 2025-01-01 Epub Date: 2024-08-09 DOI: 10.1089/fpd.2024.0055
Fengguang Dong, Guiqiang Wang, Xueying Feng, Chunbo Gong, Wenjuan Liu, Songsong Wang, Yiyi Zhang, Yapeng Huo, Youxia Chen, Hongtao Wang
{"title":"Contamination Status and Serotypes Distribution of <i>Salmonella</i> in Food in Yantai City, China: A 14-Year Continuous Monitoring Study.","authors":"Fengguang Dong, Guiqiang Wang, Xueying Feng, Chunbo Gong, Wenjuan Liu, Songsong Wang, Yiyi Zhang, Yapeng Huo, Youxia Chen, Hongtao Wang","doi":"10.1089/fpd.2024.0055","DOIUrl":"10.1089/fpd.2024.0055","url":null,"abstract":"<p><p><i>Salmonella</i> is a foodborne zoonotic pathogen that threatens food safety and public health. However, few people have conducted long-term and systematic studies on <i>Salmonella</i> contamination in food in Yantai City. In order to investigate the situation of <i>Salmonella</i> contamination in food and improve the ability of early warning and control of foodborne diseases, a total of 3420 samples from 20 categories were collected from 13 monitoring points in Yantai City, from 2010 to 2023. The difference in detection rate and bacterial strain of different monitoring points, different types, and different sources of samples was compared. Of the 3420 samples, 80 were positive with a detection rate of 2.34%. <i>Salmonella</i> detection rates were significantly different for samples collected at different monitoring sites. <i>Salmonella</i> was detected only in meat and meat products and catering food, and none of the other types were detected. The detection rate of <i>Salmonella</i> was higher in raw animal meat and raw poultry. Samples collected at the market stage had the highest detection rate (5.81%), and there was a significant difference in detection rate between samples from different sources (χ<sup>2</sup> = 36.93, <i>p</i> < 0.05). Eighty-one strains of <i>Salmonella</i> were detected out of 3420 samples (2 different strains were detected in 1 positive sample). The serological test identified 8 groups and 27 serotypes. The dominant serum groups were group B 30.86% (25/81), group E1 23.46% (19/81), and group D 16.05% (13/81). The main dominant serotypes were <i>Salmonella</i> <i>give</i> 17.28% (14/81), <i>Salmonella</i> <i>enteritidis</i> 16.05% (13/81), and <i>Salmonella</i> <i>derby</i> 13.58% (11/81). Meat and meat products and catering food were the main food products contaminated with <i>Salmonella</i>. The resulting secondary contamination is the hidden threat of foodborne diseases and should be given sufficient attention.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"15-23"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141909835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular Epidemiology of Carbapenemase-Producing Pseudomonas aeruginosa: An Iranian Referral Hospital-Based Study. 产碳青霉烯酶铜绿假单胞菌的分子流行病学:一项基于伊朗转诊医院的研究。
IF 1.9 2区 农林科学
Foodborne pathogens and disease Pub Date : 2025-01-01 Epub Date: 2024-08-29 DOI: 10.1089/fpd.2024.0066
Shima Mahmoudi, Erfaneh Jafari, Babak Pourakbari, Shiva Poormohammadi, Reihaneh Hosseinpour Sadeghi, Setareh Mamishi
{"title":"Molecular Epidemiology of Carbapenemase-Producing <i>Pseudomonas aeruginosa</i>: An Iranian Referral Hospital-Based Study.","authors":"Shima Mahmoudi, Erfaneh Jafari, Babak Pourakbari, Shiva Poormohammadi, Reihaneh Hosseinpour Sadeghi, Setareh Mamishi","doi":"10.1089/fpd.2024.0066","DOIUrl":"10.1089/fpd.2024.0066","url":null,"abstract":"<p><p>In recent years, there has been a significant increase in infections caused by carbapenemase-producing strains, with carbapenem-resistant <i>Pseudomonas aeruginosa</i> (CRPA) emerging as a priority pathogen according to the World Health Organization. This study aimed to evaluate the molecular epidemiology of CRPA isolated from patients referred to Children's Medical Center in Tehran, Iran. <i>P. aeruginosa</i> isolates collected from different children's wards were screened for common carbapenem-resistant genes by polymerase chain reaction (PCR). Genetic relatedness between isolates was assessed by pulsed-field gel electrophoresis (PFGE). The study included 133 participants, with 50% being male, and revealed a median age of 2 years (interquartile range: 6 months to 6 years). Carbapenem resistance was detected in 15% of cases (<i>n</i> = 20), with CRPA isolates predominantly found in the emergency ward (60%). The median age of patients with CRPA was significantly higher than those with carbapenem-susceptible <i>P. aeruginosa</i> (6 years vs. 1 year). PCR analysis revealed metallo-β-lactamase production in 45% of CRPA isolates (<i>n</i> = 9), with <i>bla<sub>NDM</sub></i> being the most prevalent gene. PFGE analysis of the CRPA isolates identified three clusters (Cluster I, II, and III). Cluster I, comprising 65% of all isolates (<i>n</i> = 13), was predominantly found in the emergency ward. Notably, <i>bla<sub>NDM</sub></i>-producing strains were prevalent in the emergency ward. Our study highlights the significant prevalence of CRPA in the emergency ward of our hospital and underscores the importance of targeted surveillance and infection control measures to curb its spread within health care settings.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"51-57"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Improving Cost Estimates for Risk-Based Food Safety Management and Policy. 改进基于风险的食品安全管理和政策的成本估算。
IF 1.9 2区 农林科学
Foodborne pathogens and disease Pub Date : 2025-01-01 Epub Date: 2024-10-25 DOI: 10.1089/fpd.2024.0146
Sandra A Hoffmann, Elaine J Scallan Walter
{"title":"Improving Cost Estimates for Risk-Based Food Safety Management and Policy.","authors":"Sandra A Hoffmann, Elaine J Scallan Walter","doi":"10.1089/fpd.2024.0146","DOIUrl":"10.1089/fpd.2024.0146","url":null,"abstract":"","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"1-3"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sensitive and Specific Loop-Mediated Isothermal Amplification Assays for Detection of Salmonella, CTX-M-1 Group Genes, mph(A), and ermB in Stool and Blood Samples Based on Orange to Green Visible Dye. 基于橙绿可见染料的灵敏而特异的环路介导等温扩增测定,用于检测粪便和血液样本中的沙门氏菌、CTX-M-1 组基因、mph(A) 和 ermB。
IF 1.9 2区 农林科学
Foodborne pathogens and disease Pub Date : 2025-01-01 Epub Date: 2024-03-22 DOI: 10.1089/fpd.2023.0094
Jianbo Feng, Ya Huang, Huixin Chen, Shujin Xie, Congzhu Yang, Weixin Zheng, Jinglin Su, Wentao Zheng, Jiajie Mo, Fei Lv
{"title":"Sensitive and Specific Loop-Mediated Isothermal Amplification Assays for Detection of <i>Salmonella</i>, CTX-M-1 Group Genes, <i>mph(A)</i>, and <i>ermB</i> in Stool and Blood Samples Based on Orange to Green Visible Dye.","authors":"Jianbo Feng, Ya Huang, Huixin Chen, Shujin Xie, Congzhu Yang, Weixin Zheng, Jinglin Su, Wentao Zheng, Jiajie Mo, Fei Lv","doi":"10.1089/fpd.2023.0094","DOIUrl":"10.1089/fpd.2023.0094","url":null,"abstract":"<p><p><i>Salmonella</i> is a globally prevalent foodborne bacterium, and ceftriaxone and azithromycin have been regarded as drugs of choice for treating <i>Salmonella</i> infections, particularly in children. With the growing incidence of ceftriaxone and azithromycin resistance in <i>Salmonella</i>, there is an urgent requirement for a rapid and dependable gene testing approach to enhance the efficacy of treating <i>Salmonella</i> infections. Utilizing the orange to green visible dye approach, this study developed loop-mediated isothermal amplification (LAMP) assays for the sensitive and specific detection of <i>Salmonella</i>, ceftriaxone and azithromycin resistance genes (including CTX-M-1 group, <i>mph(A)</i>, and <i>ermB</i> genes) in stool and blood samples. The specificity and sensitivity of primers during the LAMP assays for detection of <i>Salmonella</i>, CTX-M-1 group, <i>mph(A)</i>, and <i>ermB</i> genes were determined in this study. The detection threshold for <i>Salmonella</i> was found to be 1.5 × 10<sup>3</sup> colony-forming units (CFU)/mL, while it was 1.5 × 10<sup>2</sup> CFU/mL for CTX-M-1 group genes (including <i>bla</i><sub>CTX-M-3</sub>, <i>bla</i><sub>CTX-M-15</sub>, and <i>bla</i><sub>CTX-M-55</sub>), 1.5 × 10<sup>2</sup> CFU/mL for <i>mph(A)</i>, and 1.5 × 10<sup>2</sup> CFU/mL for <i>ermB</i>, showing 10-10<sup>3</sup>-fold, 10<sup>3</sup>-fold, and 10<sup>5</sup>-fold increased sensitivity compared with the polymerase chain reaction assay, respectively. Results indicated that the LAMP primers designed for <i>Salmonella</i>, CTX-M-1 group, <i>mph(A)</i>, and <i>ermB</i> genes possess high specificity (100%) and sensitivity (over 94%). This novel approach advocates its application in detecting <i>Salmonella</i>, CTX-M-1 group, <i>mph(A)</i>, and <i>ermB</i> genes.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"58-65"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140287218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phenotypic and Genotypic of Antimicrobial Resistance Profile of Staphylococcus spp. Isolated from Raw Milk and Artisanal Cheese. 从生乳和手工奶酪中分离的葡萄球菌的抗菌谱表型和基因型从生乳和手工奶酪中分离的葡萄球菌的抗菌谱。
IF 1.9 2区 农林科学
Foodborne pathogens and disease Pub Date : 2025-01-01 Epub Date: 2024-09-05 DOI: 10.1089/fpd.2024.0037
Fernanda Danielle Melo, Heloíse Peterle, Ricardo Antonio Pilegi Sfaciotte, Mateus Felipe Schneider, Leandro Parussolo, Sheila Rezler Wosiacki, Ubirajara Maciel da Costa, Sandra Maria Ferraz
{"title":"Phenotypic and Genotypic of Antimicrobial Resistance Profile of <i>Staphylococcus</i> spp. Isolated from Raw Milk and Artisanal Cheese.","authors":"Fernanda Danielle Melo, Heloíse Peterle, Ricardo Antonio Pilegi Sfaciotte, Mateus Felipe Schneider, Leandro Parussolo, Sheila Rezler Wosiacki, Ubirajara Maciel da Costa, Sandra Maria Ferraz","doi":"10.1089/fpd.2024.0037","DOIUrl":"10.1089/fpd.2024.0037","url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> and a few species of coagulase negative are frequently associated with food poisoning. Raw milk and dairy products are among the foods usually associated with outbreaks due to staphylococcal intoxication. This study aimed to determine phenotypic and genotypic antimicrobial resistance profiles to beta-lactam drugs in <i>Staphylococcus</i> coagulase positive (CoPS) and negative (CoNS) isolates. A total of 58 CoPS and 45 CoNS isolates recovered from raw milk and artisanal cheese from Santa Catarina were analyzed. All isolates (<i>n</i> = 103) were subjected to antimicrobial susceptibility testing. High levels of resistance to penicillin (41% of CoPS and 31% of CoNS), amoxicillin (40% CoPS), ampicillin (36% CoPS), and sulfamethoxazole-trimethoprim (35% CoNS) were observed. Twenty six percent of the isolates (18 CoPS and 9 CoNS) exhibited multiresistance profile; which means, they were resistant to at least three different classes of the antimicrobial drugs. Detection of resistance genes (<i>mec</i>A, <i>mec</i>C, and <i>bla</i>Z) was performed using multiplex polymerase chain reaction. Twelve isolates (9 CoPS and 3 CoNS) were positive for <i>mec</i>A, whereas 10 strains (4 CoPS and 6 CoNS) were positive for <i>bla</i>Z. The detection of resistant and multidrug resistant isolates emphasizes the necessity to develop strategies to better comply with good manufacturing practices and health care guidelines.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"24-30"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antimicrobial Resistance and Public Health Risks Associated with Staphylococci Isolated from Raw and Processed Meat Products. 从生肉制品和加工肉制品中分离出的葡萄球菌的抗菌性和公共卫生风险。
IF 1.9 2区 农林科学
Foodborne pathogens and disease Pub Date : 2025-01-01 Epub Date: 2024-09-23 DOI: 10.1089/fpd.2024.0081
Mohammad Anas, Showkat Ahmad Lone, Abdul Malik, Junaid Ahmad
{"title":"Antimicrobial Resistance and Public Health Risks Associated with Staphylococci Isolated from Raw and Processed Meat Products.","authors":"Mohammad Anas, Showkat Ahmad Lone, Abdul Malik, Junaid Ahmad","doi":"10.1089/fpd.2024.0081","DOIUrl":"10.1089/fpd.2024.0081","url":null,"abstract":"<p><p>This study aimed at evaluating the occurrence, antibiotic resistance, and β-lactamase production in <i>Staphylococcus</i> isolates recovered from meat and meat products, as well as the incidence of antimicrobial resistance (AMR) genes in these bacterial isolates. The prevalence of <i>Staphylococcus</i> was very high (75% and 50%) in street kebab and raw buffalo meat, respectively. The antibiotic resistance and susceptibility behavior showed that 82% of the <i>Staphylococcus</i> isolates were resistant to β-lactam antibiotics such as aztreonam, followed by methicillin (68%), oxacillin (54%), cefepime (36%), ceftazidime (34%), cefaclor (24%), cefotaxime (22%), ertapenem (4%), meropenem and imipenem (2%). Among non-β-lactam antibiotics, the most widespread resistance was observed against nalidixic acid (80%), sulfadiazine (76%), vancomycin (24%), erythromycin (10%), chloramphenicol (6%), and kanamycin and gatifloxacin (4%). One hundred percent of the isolates were susceptible to ciprofloxacin, tetracycline, gemifloxacin, and cefotaxime/clavulanic acid. <i>In vitro</i> drug-resistant characteristics revealed 36 distinct resistance patterns of <i>Staphylococcus</i> isolates, with 82% of them being multidrug resistant (MDR). Iodometric assay showed that 48% of the <i>Staphylococcus</i> isolates produced β-lactamase and 24% of the isolates were capable of producing extended-spectrum beta-lactamases phenotypically. The most commonly detected AMR gene was <i>mecA</i> (29.2%), followed by <i>Sul</i> 1 (25%) and <i>qnrS</i> and <i>qnrB</i> (20.8%), in <i>Staphylococcus</i> isolates. Current findings show widespread occurrence of MDR <i>Staphylococcus</i> strains in raw meat and street meat products, which is a potential risk to public health. Therefore, the study suggests strict monitoring of hygiene through the whole food chain and judicious use of antibiotics.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"39-50"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142282794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phylogenetic Characterization of Vibrio vulnificus Strains from Oysters and its Comparison with Clinical vcgE Genotype Strain. 牡蛎弧菌菌株的系统发育特征及其与临床 vcgE 基因型菌株的比较。
IF 1.9 2区 农林科学
Foodborne pathogens and disease Pub Date : 2025-01-01 Epub Date: 2024-11-26 DOI: 10.1089/fpd.2024.0035
Abraham Guerrero, Clara Elizabeth Galindo-Sánchez, Ana Verónica Martínez-Vázquez, Marcial Leonardo Lizárraga-Partida
{"title":"Phylogenetic Characterization of <i>Vibrio vulnificus</i> Strains from Oysters and its Comparison with Clinical <i>vcg</i>E Genotype Strain.","authors":"Abraham Guerrero, Clara Elizabeth Galindo-Sánchez, Ana Verónica Martínez-Vázquez, Marcial Leonardo Lizárraga-Partida","doi":"10.1089/fpd.2024.0035","DOIUrl":"10.1089/fpd.2024.0035","url":null,"abstract":"<p><p>Twenty-one environmental <i>Vibrio vulnificus</i> strains from the Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE) bacteria collection, 20 of them isolated from oyster samples and 1 from a reported clinical case, were sequenced to analyze the genomic divergence between 2 genotypes, E-genotype and C-genotype, proposed by various groups to distinguish clinical (C) from environmental (E) <i>V. vulnificus</i> strains. As indicated in previous analyses of PFGE, MLST, and <i>rtx</i>A, 9 of the CICESE isolates were identified as <i>vcg</i>E, compared with 12 as <i>vcg</i>C. Separation of the genotypes into these 2 groups was confirmed in this study, based on the presence of certain genes in the 21 genomes, the presence of virulence factors, and <i>rtx</i>A sequencing. Most genomes from oyster isolates expressed <i>rtx</i>A-C type, with the exception being <i>rtx</i>A-M type detected in CICESE-594 a <i>vcg</i>E strain isolated from a clinical case. Although several genetic approaches clearly indicate differences between the C- and E-genotypes, none of them, including those in this study, can highlight a single factor that could be used to indicate the potential pathogenicity of <i>V. vulnificus</i> isolated from oysters.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"31-38"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11839520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Serogroups and Toxin Variants of Clinical Shiga Toxin-Producing Escherichia coli Strains Isolated from Patients with Hemolytic Uremic Syndrome in Turkey. 土耳其溶血性尿毒症患者临床产志贺毒素大肠杆菌菌株的血清群和毒素变异
IF 1.9 2区 农林科学
Foodborne pathogens and disease Pub Date : 2024-12-27 DOI: 10.1089/fpd.2024.0140
Elif Okumuş, Aynur Karadenizli, Elif Bahat Özdoğan, Kenan Bek
{"title":"Serogroups and Toxin Variants of Clinical Shiga Toxin-Producing <i>Escherichia coli</i> Strains Isolated from Patients with Hemolytic Uremic Syndrome in Turkey.","authors":"Elif Okumuş, Aynur Karadenizli, Elif Bahat Özdoğan, Kenan Bek","doi":"10.1089/fpd.2024.0140","DOIUrl":"https://doi.org/10.1089/fpd.2024.0140","url":null,"abstract":"<p><p>Shiga toxin-producing <i>Escherichia coli</i> (STEC) refers to a group of bacteria that can cause infections, which are common worldwide and pose a serious public health problem, as they can lead to conditions such as hemorrhagic colitis and hemolytic uremic syndrome (HUS). HUS is a disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, and renal failure. Determination of serogroups and toxin profiles of STEC is important for estimating their disease-causing potential and predicting epidemiological changes. This study analyzed STEC isolates from 46 pediatric HUS patients across Turkey, using polymerase chain reaction to determine O serogroups and Shiga toxin (Stx) variants from stool samples collected between 2016 and 2019. Of the patients, 25 (54.3%) were in the 0-2 age group. Of the isolates, 82.6% were non-O157 serogroup. The most detected serogroup was O145 (32.6%), and 28.3% of the serogroups were not typed. Of the strains, 8 (17.4%) had Stx1 alone, 26 (56.5%) had Stx2 alone, and 12 (26.1%) had both Stx1 and Stx2. The Stx variants occurred in seven combinations, with the most common being Stx2a alone (56.5%). The duration of hospitalization for patients with Stx2a was found to be longer than that for patients with other variants (<i>p</i> = 0.01). This study highlights a concerning trend in Turkey, where non-O157 serogroups, particularly O145, emerged as prevalent causes of HUS. The predominance of Stx2a among our isolates and the longer hospitalization duration for patients with Stx2a support findings linking this variant to severe clinical outcomes, including HUS. Understanding the dynamics of these Stx variants will help better prepare for and mitigate the impacts of STEC infections in our population.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142893212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Oral Immunization of Mice with a Nonantibiotic Recombinant Lactobacillus plantarum Coexpressing Trichinella spiralis TsNd and gp43 Protein Vaccine Combined with a Murine Interleukin-33 Adjuvant. 非抗生素重组植物乳杆菌共表达旋毛虫TsNd和gp43蛋白疫苗联合小鼠白细胞介素-33佐剂口服免疫小鼠
IF 1.9 2区 农林科学
Foodborne pathogens and disease Pub Date : 2024-12-24 DOI: 10.1089/fpd.2024.0125
Hong-Ye Li, Dan Wang, Qiu-Yu Xie, Xin Liu, Jun-Yi Li, Hai-Bin Huang, Nan Wang, Chun-Wei Shi, Tian-Xu Pan, Ming-Han Li, Dong-Yu Zhao, Shu-Yuan Yu, Jia-Yao Guan, Gui-Lian Yang
{"title":"Oral Immunization of Mice with a Nonantibiotic Recombinant <i>Lactobacillus plantarum</i> Coexpressing <i>Trichinella spiralis</i> TsNd and gp43 Protein Vaccine Combined with a Murine Interleukin-33 Adjuvant.","authors":"Hong-Ye Li, Dan Wang, Qiu-Yu Xie, Xin Liu, Jun-Yi Li, Hai-Bin Huang, Nan Wang, Chun-Wei Shi, Tian-Xu Pan, Ming-Han Li, Dong-Yu Zhao, Shu-Yuan Yu, Jia-Yao Guan, Gui-Lian Yang","doi":"10.1089/fpd.2024.0125","DOIUrl":"https://doi.org/10.1089/fpd.2024.0125","url":null,"abstract":"<p><p>Trichinellosis, a zoonotic disease transmitted through food and caused by <i>Trichinella spiralis</i>, is a significant health concern worldwide. Therefore, developing a safe and effective vaccine to combat <i>T. spiralis</i> infection is essential. In this study, a nonantibiotic <i>Lactobacillus plantarum</i> strain lacking the <i>alr</i> gene served as a live bacterial vector to deliver antigens to the host, creating a novel oral vaccine. A nonantibiotic vaccine was constructed to coexpress <i>T. spiralis</i> Nudix hydrolase (TsNd protein) and the gp43 protein alongside a food-grade murine IL-33 adjuvant. The objective of this study was to evaluate the efficacy of this novel strategy in preventing trichinellosis and mitigating intestinal damage. The integrated vaccination strategy was found to increase specific serum immunoglobulin G and mucosal secretory IgA (sIgA) concentration in BALB/c mice. Additionally, this approach significantly increased the release of cytokines such as interleukin-4 (IL-4) from lymphocytes, along with increased CD4<sup>+</sup> expression on lymph cells from both splenic and mesenteric sources. Post-larval exposure, significant reductions in the populations of adult worms by 59.80% at 7 d postinfection (dpi) and in the larval load by 51.29% at 28 dpi were documented. Furthermore, oral vaccination reduced the number of encysted larvae present in the tongue and masseter muscles after challenge with <i>T. spiralis</i>. Overall, the results demonstrated that combined vaccination with the IL-33 adjuvant effectively prevented trichinellosis and reduced intestinal damage, highlighting the potential of food-grade <i>Lactobacillus</i> vaccines in preventing intestinal parasitic infections, with IL-33 serving as an effective adjuvant.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142881475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信