Foodborne pathogens and disease最新文献

{"title":"<i>Listeria monocytogenes, Salmonella</i> spp., and <i>Staphylococcus aureus:</i> Threats to the Food Industry and Public Health.","authors":"Vinicius B Mantovam, David F Dos Santos, Luis C Giola Junior, Mariza Landgraf, Uelinton M Pinto, Svetoslav D Todorov","doi":"10.1089/fpd.2024.0124","DOIUrl":"https://doi.org/10.1089/fpd.2024.0124","url":null,"abstract":"<p><p>Foodborne pathogens have always been of public health concern and represent safety issues for food processors. These pathogens develop new ways to overcome antibiotics, survive in different environmental conditions, and the ability to reproduce in many hostile environments configure them as serious health hazards. Considering the huge number of microorganisms, three bacterial representatives were selected to provide a better knowledge about the question of which one is the worst enemy for humans, from the food industry point of view, taking into consideration their multiplication specificity, virulence, and resistance. As we constantly are exposed to these pathogens in our nutritional habits, this overview aims to summarize the most relevant characteristics associated with the pathogenicity, clinical symptoms and most importantly, how deadly <i>Listeria monocytogenes</i>, <i>Salmonella</i> spp., and <i>Staphylococcus aureus</i> can be in the hospital and the food industry, by comparing among them. Overall, the microbiological knowledge clearly suggests that while all three pathogens are dangerous, <i>L. monocytogenes</i> presents the highest risk of death due to their ability to cause severe complications in vulnerable populations as it presents a range of virulence factors that facilitate evasion of the immune system and cytological effects. Additionally, it shows great resistance to standard food processing and preservation techniques, making it one of the most difficult pathogens to control. Understanding the risks and characteristics of these foodborne pathogens is essential for implementing effective control measures to prevent their occurrence in food products and to promote public health.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142931107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acknowledgment of Reviewers 2024.","authors":"","doi":"10.1089/fpd.2024.84560.revack","DOIUrl":"https://doi.org/10.1089/fpd.2024.84560.revack","url":null,"abstract":"","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":"22 1","pages":"66-69"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contamination Status and Serotypes Distribution of <i>Salmonella</i> in Food in Yantai City, China: A 14-Year Continuous Monitoring Study.","authors":"Fengguang Dong, Guiqiang Wang, Xueying Feng, Chunbo Gong, Wenjuan Liu, Songsong Wang, Yiyi Zhang, Yapeng Huo, Youxia Chen, Hongtao Wang","doi":"10.1089/fpd.2024.0055","DOIUrl":"10.1089/fpd.2024.0055","url":null,"abstract":"<p><p><i>Salmonella</i> is a foodborne zoonotic pathogen that threatens food safety and public health. However, few people have conducted long-term and systematic studies on <i>Salmonella</i> contamination in food in Yantai City. In order to investigate the situation of <i>Salmonella</i> contamination in food and improve the ability of early warning and control of foodborne diseases, a total of 3420 samples from 20 categories were collected from 13 monitoring points in Yantai City, from 2010 to 2023. The difference in detection rate and bacterial strain of different monitoring points, different types, and different sources of samples was compared. Of the 3420 samples, 80 were positive with a detection rate of 2.34%. <i>Salmonella</i> detection rates were significantly different for samples collected at different monitoring sites. <i>Salmonella</i> was detected only in meat and meat products and catering food, and none of the other types were detected. The detection rate of <i>Salmonella</i> was higher in raw animal meat and raw poultry. Samples collected at the market stage had the highest detection rate (5.81%), and there was a significant difference in detection rate between samples from different sources (χ² = 36.93, <i>p</i> < 0.05). Eighty-one strains of <i>Salmonella</i> were detected out of 3420 samples (2 different strains were detected in 1 positive sample). The serological test identified 8 groups and 27 serotypes. The dominant serum groups were group B 30.86% (25/81), group E1 23.46% (19/81), and group D 16.05% (13/81). The main dominant serotypes were <i>Salmonella</i> <i>give</i> 17.28% (14/81), <i>Salmonella</i> <i>enteritidis</i> 16.05% (13/81), and <i>Salmonella</i> <i>derby</i> 13.58% (11/81). Meat and meat products and catering food were the main food products contaminated with <i>Salmonella</i>. The resulting secondary contamination is the hidden threat of foodborne diseases and should be given sufficient attention.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"15-23"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141909835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Epidemiology of Carbapenemase-Producing <i>Pseudomonas aeruginosa</i>: An Iranian Referral Hospital-Based Study.","authors":"Shima Mahmoudi, Erfaneh Jafari, Babak Pourakbari, Shiva Poormohammadi, Reihaneh Hosseinpour Sadeghi, Setareh Mamishi","doi":"10.1089/fpd.2024.0066","DOIUrl":"10.1089/fpd.2024.0066","url":null,"abstract":"<p><p>In recent years, there has been a significant increase in infections caused by carbapenemase-producing strains, with carbapenem-resistant <i>Pseudomonas aeruginosa</i> (CRPA) emerging as a priority pathogen according to the World Health Organization. This study aimed to evaluate the molecular epidemiology of CRPA isolated from patients referred to Children's Medical Center in Tehran, Iran. <i>P. aeruginosa</i> isolates collected from different children's wards were screened for common carbapenem-resistant genes by polymerase chain reaction (PCR). Genetic relatedness between isolates was assessed by pulsed-field gel electrophoresis (PFGE). The study included 133 participants, with 50% being male, and revealed a median age of 2 years (interquartile range: 6 months to 6 years). Carbapenem resistance was detected in 15% of cases (<i>n</i> = 20), with CRPA isolates predominantly found in the emergency ward (60%). The median age of patients with CRPA was significantly higher than those with carbapenem-susceptible <i>P. aeruginosa</i> (6 years vs. 1 year). PCR analysis revealed metallo-β-lactamase production in 45% of CRPA isolates (<i>n</i> = 9), with <i>bla_NDM</i> being the most prevalent gene. PFGE analysis of the CRPA isolates identified three clusters (Cluster I, II, and III). Cluster I, comprising 65% of all isolates (<i>n</i> = 13), was predominantly found in the emergency ward. Notably, <i>bla_NDM</i>-producing strains were prevalent in the emergency ward. Our study highlights the significant prevalence of CRPA in the emergency ward of our hospital and underscores the importance of targeted surveillance and infection control measures to curb its spread within health care settings.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"51-57"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving Cost Estimates for Risk-Based Food Safety Management and Policy.","authors":"Sandra A Hoffmann, Elaine J Scallan Walter","doi":"10.1089/fpd.2024.0146","DOIUrl":"10.1089/fpd.2024.0146","url":null,"abstract":"","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"1-3"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142497814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitive and Specific Loop-Mediated Isothermal Amplification Assays for Detection of <i>Salmonella</i>, CTX-M-1 Group Genes, <i>mph(A)</i>, and <i>ermB</i> in Stool and Blood Samples Based on Orange to Green Visible Dye.","authors":"Jianbo Feng, Ya Huang, Huixin Chen, Shujin Xie, Congzhu Yang, Weixin Zheng, Jinglin Su, Wentao Zheng, Jiajie Mo, Fei Lv","doi":"10.1089/fpd.2023.0094","DOIUrl":"10.1089/fpd.2023.0094","url":null,"abstract":"<p><p><i>Salmonella</i> is a globally prevalent foodborne bacterium, and ceftriaxone and azithromycin have been regarded as drugs of choice for treating <i>Salmonella</i> infections, particularly in children. With the growing incidence of ceftriaxone and azithromycin resistance in <i>Salmonella</i>, there is an urgent requirement for a rapid and dependable gene testing approach to enhance the efficacy of treating <i>Salmonella</i> infections. Utilizing the orange to green visible dye approach, this study developed loop-mediated isothermal amplification (LAMP) assays for the sensitive and specific detection of <i>Salmonella</i>, ceftriaxone and azithromycin resistance genes (including CTX-M-1 group, <i>mph(A)</i>, and <i>ermB</i> genes) in stool and blood samples. The specificity and sensitivity of primers during the LAMP assays for detection of <i>Salmonella</i>, CTX-M-1 group, <i>mph(A)</i>, and <i>ermB</i> genes were determined in this study. The detection threshold for <i>Salmonella</i> was found to be 1.5 × 10³ colony-forming units (CFU)/mL, while it was 1.5 × 10² CFU/mL for CTX-M-1 group genes (including <i>bla</i>_CTX-M-3, <i>bla</i>_CTX-M-15, and <i>bla</i>_CTX-M-55), 1.5 × 10² CFU/mL for <i>mph(A)</i>, and 1.5 × 10² CFU/mL for <i>ermB</i>, showing 10-10³-fold, 10³-fold, and 10⁵-fold increased sensitivity compared with the polymerase chain reaction assay, respectively. Results indicated that the LAMP primers designed for <i>Salmonella</i>, CTX-M-1 group, <i>mph(A)</i>, and <i>ermB</i> genes possess high specificity (100%) and sensitivity (over 94%). This novel approach advocates its application in detecting <i>Salmonella</i>, CTX-M-1 group, <i>mph(A)</i>, and <i>ermB</i> genes.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"58-65"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140287218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic and Genotypic of Antimicrobial Resistance Profile of <i>Staphylococcus</i> spp. Isolated from Raw Milk and Artisanal Cheese.","authors":"Fernanda Danielle Melo, Heloíse Peterle, Ricardo Antonio Pilegi Sfaciotte, Mateus Felipe Schneider, Leandro Parussolo, Sheila Rezler Wosiacki, Ubirajara Maciel da Costa, Sandra Maria Ferraz","doi":"10.1089/fpd.2024.0037","DOIUrl":"10.1089/fpd.2024.0037","url":null,"abstract":"<p><p><i>Staphylococcus aureus</i> and a few species of coagulase negative are frequently associated with food poisoning. Raw milk and dairy products are among the foods usually associated with outbreaks due to staphylococcal intoxication. This study aimed to determine phenotypic and genotypic antimicrobial resistance profiles to beta-lactam drugs in <i>Staphylococcus</i> coagulase positive (CoPS) and negative (CoNS) isolates. A total of 58 CoPS and 45 CoNS isolates recovered from raw milk and artisanal cheese from Santa Catarina were analyzed. All isolates (<i>n</i> = 103) were subjected to antimicrobial susceptibility testing. High levels of resistance to penicillin (41% of CoPS and 31% of CoNS), amoxicillin (40% CoPS), ampicillin (36% CoPS), and sulfamethoxazole-trimethoprim (35% CoNS) were observed. Twenty six percent of the isolates (18 CoPS and 9 CoNS) exhibited multiresistance profile; which means, they were resistant to at least three different classes of the antimicrobial drugs. Detection of resistance genes (<i>mec</i>A, <i>mec</i>C, and <i>bla</i>Z) was performed using multiplex polymerase chain reaction. Twelve isolates (9 CoPS and 3 CoNS) were positive for <i>mec</i>A, whereas 10 strains (4 CoPS and 6 CoNS) were positive for <i>bla</i>Z. The detection of resistant and multidrug resistant isolates emphasizes the necessity to develop strategies to better comply with good manufacturing practices and health care guidelines.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"24-30"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial Resistance and Public Health Risks Associated with Staphylococci Isolated from Raw and Processed Meat Products.","authors":"Mohammad Anas, Showkat Ahmad Lone, Abdul Malik, Junaid Ahmad","doi":"10.1089/fpd.2024.0081","DOIUrl":"10.1089/fpd.2024.0081","url":null,"abstract":"<p><p>This study aimed at evaluating the occurrence, antibiotic resistance, and β-lactamase production in <i>Staphylococcus</i> isolates recovered from meat and meat products, as well as the incidence of antimicrobial resistance (AMR) genes in these bacterial isolates. The prevalence of <i>Staphylococcus</i> was very high (75% and 50%) in street kebab and raw buffalo meat, respectively. The antibiotic resistance and susceptibility behavior showed that 82% of the <i>Staphylococcus</i> isolates were resistant to β-lactam antibiotics such as aztreonam, followed by methicillin (68%), oxacillin (54%), cefepime (36%), ceftazidime (34%), cefaclor (24%), cefotaxime (22%), ertapenem (4%), meropenem and imipenem (2%). Among non-β-lactam antibiotics, the most widespread resistance was observed against nalidixic acid (80%), sulfadiazine (76%), vancomycin (24%), erythromycin (10%), chloramphenicol (6%), and kanamycin and gatifloxacin (4%). One hundred percent of the isolates were susceptible to ciprofloxacin, tetracycline, gemifloxacin, and cefotaxime/clavulanic acid. <i>In vitro</i> drug-resistant characteristics revealed 36 distinct resistance patterns of <i>Staphylococcus</i> isolates, with 82% of them being multidrug resistant (MDR). Iodometric assay showed that 48% of the <i>Staphylococcus</i> isolates produced β-lactamase and 24% of the isolates were capable of producing extended-spectrum beta-lactamases phenotypically. The most commonly detected AMR gene was <i>mecA</i> (29.2%), followed by <i>Sul</i> 1 (25%) and <i>qnrS</i> and <i>qnrB</i> (20.8%), in <i>Staphylococcus</i> isolates. Current findings show widespread occurrence of MDR <i>Staphylococcus</i> strains in raw meat and street meat products, which is a potential risk to public health. Therefore, the study suggests strict monitoring of hygiene through the whole food chain and judicious use of antibiotics.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"39-50"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142282794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenetic Characterization of <i>Vibrio vulnificus</i> Strains from Oysters and its Comparison with Clinical <i>vcg</i>E Genotype Strain.","authors":"Abraham Guerrero, Clara Elizabeth Galindo-Sánchez, Ana Verónica Martínez-Vázquez, Marcial Leonardo Lizárraga-Partida","doi":"10.1089/fpd.2024.0035","DOIUrl":"10.1089/fpd.2024.0035","url":null,"abstract":"<p><p>Twenty-one environmental <i>Vibrio vulnificus</i> strains from the Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE) bacteria collection, 20 of them isolated from oyster samples and 1 from a reported clinical case, were sequenced to analyze the genomic divergence between 2 genotypes, E-genotype and C-genotype, proposed by various groups to distinguish clinical (C) from environmental (E) <i>V. vulnificus</i> strains. As indicated in previous analyses of PFGE, MLST, and <i>rtx</i>A, 9 of the CICESE isolates were identified as <i>vcg</i>E, compared with 12 as <i>vcg</i>C. Separation of the genotypes into these 2 groups was confirmed in this study, based on the presence of certain genes in the 21 genomes, the presence of virulence factors, and <i>rtx</i>A sequencing. Most genomes from oyster isolates expressed <i>rtx</i>A-C type, with the exception being <i>rtx</i>A-M type detected in CICESE-594 a <i>vcg</i>E strain isolated from a clinical case. Although several genetic approaches clearly indicate differences between the C- and E-genotypes, none of them, including those in this study, can highlight a single factor that could be used to indicate the potential pathogenicity of <i>V. vulnificus</i> isolated from oysters.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":"31-38"},"PeriodicalIF":1.9,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11839520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serogroups and Toxin Variants of Clinical Shiga Toxin-Producing <i>Escherichia coli</i> Strains Isolated from Patients with Hemolytic Uremic Syndrome in Turkey.","authors":"Elif Okumuş, Aynur Karadenizli, Elif Bahat Özdoğan, Kenan Bek","doi":"10.1089/fpd.2024.0140","DOIUrl":"https://doi.org/10.1089/fpd.2024.0140","url":null,"abstract":"<p><p>Shiga toxin-producing <i>Escherichia coli</i> (STEC) refers to a group of bacteria that can cause infections, which are common worldwide and pose a serious public health problem, as they can lead to conditions such as hemorrhagic colitis and hemolytic uremic syndrome (HUS). HUS is a disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, and renal failure. Determination of serogroups and toxin profiles of STEC is important for estimating their disease-causing potential and predicting epidemiological changes. This study analyzed STEC isolates from 46 pediatric HUS patients across Turkey, using polymerase chain reaction to determine O serogroups and Shiga toxin (Stx) variants from stool samples collected between 2016 and 2019. Of the patients, 25 (54.3%) were in the 0-2 age group. Of the isolates, 82.6% were non-O157 serogroup. The most detected serogroup was O145 (32.6%), and 28.3% of the serogroups were not typed. Of the strains, 8 (17.4%) had Stx1 alone, 26 (56.5%) had Stx2 alone, and 12 (26.1%) had both Stx1 and Stx2. The Stx variants occurred in seven combinations, with the most common being Stx2a alone (56.5%). The duration of hospitalization for patients with Stx2a was found to be longer than that for patients with other variants (<i>p</i> = 0.01). This study highlights a concerning trend in Turkey, where non-O157 serogroups, particularly O145, emerged as prevalent causes of HUS. The predominance of Stx2a among our isolates and the longer hospitalization duration for patients with Stx2a support findings linking this variant to severe clinical outcomes, including HUS. Understanding the dynamics of these Stx variants will help better prepare for and mitigate the impacts of STEC infections in our population.</p>","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142893212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}