Phenotypic and Whole-Genome Sequencing-Based Profiling of Antimicrobial Resistance and Virulence in Campylobacter spp. Isolated from Human Stool in a Croatian University Hospital.

IF 1.9 2区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY

Foodborne pathogens and disease Pub Date : 2025-03-07 DOI:10.1089/fpd.2024.0173

Luka Katic, Ivana Ferenčak, Ana Gverić-Grginić, Ivana Goic-Barisic, Zana Rubic, Marina Radic-Skelin, Jelena Marinovic, Dora Smolić, Josipa Kuzle, Lucija Škara Abramović, Irena Tabain, Dragan Jurić, Marija Tonkic, Georgina Osorio, Anita Novak

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Abstract

Campylobacter spp. is a leading cause of community-acquired gastroenteritis, with rising antimicrobial resistance (AMR) posing a significant public health challenge. This study aimed to characterize the genomic basis of resistance and virulence in Campylobacter spp. isolated from human stool at the University Hospital of Split, Croatia. A total of 115 unduplicated isolates (100 Campylobacter jejuni and 15 Campylobacter coli) were identified and tested for antimicrobial susceptibility, from January to June 2023. A representative number of isolates with phenotypically detected resistance were analyzed with whole-genome sequencing. Multilocus sequence typing (MLST) was determined using genomic sequences as input data against the standard MLST schemes. High rates of resistance were detected to tetracycline (45% of C. jejuni and 26.7% of C. coli) and ciprofloxacin (81% of C. jejuni and 46.7% of C. coli), while combined ciprofloxacin/tetracycline resistance was observed in 43% of C. jejuni and 7% of C. coli isolates. The majority of whole-genome sequenced isolates possess tet(O/32/O) gene and gyrA.T861 mutation, conferring resistance to tetracycline and fluoroquinolones. All isolates have at least one resistance gene for β-lactams (bla_OXA-61, bla_OXA-193, bla_OXA-450, bla_OXA-451, bla_OXA-452, bla_OXA-461, and bla_OXA-489), while 40% of isolates possess ant(6)-Ia for aminoglycoside resistance. In addition, a newly emerged, phenotypically identified multidrug-resistant C. coli, harbored tet(O/32/O) and tet(O) genes, point mutation in gyrA gene, and A2075G mutation in 23S rRNA. Virulence analysis highlighted C. jejuni's broad pathogenic potential, including motility, adherence, invasion, exotoxin production, and immune modulation (e.g., wlaN gene, linked to Guillain-Barré syndrome). On the contrary, C. coli isolates possess genes exclusively for motility. Nine different C. jejuni MLST sequence types were identified, while all C. coli isolates belong to the same 828 clonal complex. Escalating AMR and a broad spectrum of virulence in Campylobacter spp. highlight the importance of continuous surveillance on the phenotypic and genotypic level, thereby allowing more efficient health care management of these infections.

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克罗地亚大学医院人类粪便中分离的弯曲杆菌的耐药性和毒力的表型和全基因组测序分析。

弯曲杆菌属是社区获得性胃肠炎的主要病因，其抗菌药耐药性（AMR）不断上升，对公共卫生构成了重大挑战。本研究旨在分析克罗地亚斯普利特大学医院从人类粪便中分离出的弯曲杆菌的耐药性和毒力的基因组基础。从 2023 年 1 月至 6 月，共鉴定并检测了 115 个不重复的分离株（100 个空肠弯曲菌和 15 个大肠弯曲菌）的抗菌药敏感性。通过全基因组测序分析了表型检测出耐药性的代表性分离物。利用基因组序列作为输入数据，对照标准多焦点序列分型（MLST）方案，确定了多焦点序列分型（MLST）。对四环素（45% 的空肠球菌和 26.7% 的大肠球菌）和环丙沙星（81% 的空肠球菌和 46.7% 的大肠球菌）的耐药率较高，而在 43% 的空肠球菌和 7% 的大肠球菌分离物中观察到了对环丙沙星/四环素的联合耐药。大多数全基因组测序的分离菌株具有 tet（O/32/O）基因和 gyrA.T861 突变，对四环素和氟喹诺酮类药物产生耐药性。所有分离株都至少有一个对β-内酰胺类药物的耐药基因（blaOXA-61、blaOXA-193、blaOXA-450、blaOXA-451、blaOXA-452、blaOXA-461 和 blaOXA-489），而 40% 的分离株具有对氨基糖苷类药物耐药的 ant（6）-Ia 基因。此外，一种新出现的表型鉴定为多重耐药的大肠杆菌含有 tet（O/32/O）和 tet（O）基因、gyrA 基因的点突变和 23S rRNA 的 A2075G 突变。病毒性分析凸显了空肠大肠杆菌的广泛致病性，包括运动性、粘附性、侵袭性、外毒素产生和免疫调节（如与格林-巴利综合征有关的 wlaN 基因）。相反，大肠杆菌分离株只拥有运动基因。已发现九种不同的空肠大肠杆菌 MLST 序列类型，而所有大肠杆菌分离株都属于同一个 828 克隆复合体。弯曲杆菌属中不断升级的 AMR 和广泛的毒力谱凸显了在表型和基因型水平上进行持续监测的重要性，从而可以更有效地对这些感染进行医疗管理。

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来源期刊

Foodborne pathogens and disease 医学-食品科技

CiteScore

5.30

自引率

3.60%

发文量

审稿时长

1 months

期刊介绍： Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.