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IF 1.9 2区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY

Foodborne pathogens and disease Pub Date : 2025-02-24 DOI:10.1089/fpd.2024.0083

Yunyi Zhang, Junyan Zhang, Jiancai Chen, Xiaojuan Qi, Zhen Zhang, Honghu Chen, Beibei Wu, Li Zhan, Jie Chen

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引用次数: 0

摘要

单核细胞增生李斯特菌（L. monocytogenes）是一种重要的食源性病原体。本研究收集了2016-2022年间的41例散发性李斯特菌病病例，包括92.7%的侵袭性病例和56.1%的妊娠相关病例。病例年龄从0天到88岁不等，大多数病例发生在20岁至单核细胞增生性李氏杆菌分离株之间，其次是1/2 a、4 b和3a。确定了 16 个克隆复合体（CC）。CC87占据首位。基于基因组测序的系统发育结果表明，中国的CC1、CC8和CC87分离物大多聚类在与其他国家分离物分离的支系中。同时，少数中国分离株参与了亚洲、欧洲、非洲、南美洲、北美洲和大洋洲的CC1、CC8和CC87的共同循环。所有分离株都携带 LIPI-1，而不携带 LIPI-2。LIPI-3 和 LIPI-4 与品系和 CC 有明显的关系。所有分离物中都存在 inlB、inlC、inlH、inlK、ipeA、srtA、dltA、lap、ami、fbpA、stp、oatA、intA、prsA2、lgt、hpt、iplA1、bsh、mdrT、mdrM 和 brtA。inlA、inlF、inlJ、aut、vip 的比例分别为 97.7%、97.7%、97.7%、83.7% 和 83.7%。1474位的过早终止密码子突变（C→T）导致了491 aa的截短InlA。对青霉素（100%）、氨苄西林（100%）、庆大霉素（100%）、红霉素（100%）、达托霉素（100%）、美罗培南（100%）、三甲双氨-磺胺甲噁唑（100%）、万古霉素（97.此外，还检测到了 tetM 和 ermB。这项全面的研究丰富了人们对李斯特菌病病例和临床分离株多样性的认识，同时基于系统发育分析指出了CC1、CC8和CC87在中国的传播特点，为制定有针对性的食品安全干预措施预防李斯特菌病提供了基础数据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Sporadic Listeriosis Cases, Genotypic Characteristics, and Antibiotic Resistance of Associated Listeria monocytogenes Isolates in Zhejiang Province, China (2016 - 2022).

Listeria monocytogenes (L. monocytogenes) is an important foodborne pathogen. In this study, 41 sporadic listeriosis cases were collected during 2016-2022, including 92.7% of invasive cases and 56.1% of pregnancy-associated cases. The age of cases ranged from 0 days to 88 years, with the majority occurring in individuals aged 20 to <30 years. Serotype 1/2 b was most prevalent among 43 L. monocytogenes isolates, followed by 1/2 a, 4 b, and 3a. Sixteen clonal complexes (CCs) were determined. CC87 occupied the top slot. Genome sequencing-based phylogeny results indicated that Chinese CC1, CC8, and CC87 isolates mostly clustered in clades separating from isolates from other countries. Meanwhile, a few Chinese isolates participated in cocirculating CC1, CC8, and CC87 in Asia, Europe, Africa, South America, North America, and Oceania. All isolates harbored LIPI-1 while LIPI-2 was absent. LIPI-3 and LIPI-4 exhibited an apparent relationship with lineage and CCs. It was notably that CC4, CC224, and CC619 carried both LIPI-3 and LIPI-4. inlB, inlC, inlH, inlK, ipeA, srtA, dltA, lap, ami, fbpA, stp, oatA, intA, prsA2, lgt, hpt, iplA1, bsh, mdrT, mdrM, and brtA existed in all isolates. The percentages of inlA, inlF, inlJ, aut, vip were 97.7%, 97.7%, 97.7%, 83.7%, and 83.7%. A premature stop codon mutation of position 1474(C→T) was detected, resulting in a truncated InlA with 491 aa. High susceptibility to penicillin (100%), ampicillin (100%), gentamicin (100%), erythromycin (100%), daptomycin (100%), meropenem (100%), trimethoprim-sulfamethoxazole (100%), vancomycin (97.7%), tetacycline (97.7%), chloramphenicol (97.7%), and ciprofloxacin (90.7%) was demonstrated. lin and fosX were present in 93.0% of the isolates, respectively. tetM and ermB were also detected. This comprehensive study enriched the understanding of listeriosis cases and diversity of clinical isolates, meanwhile, indicated the spread characteristics of CC1, CC8, and CC87 in China based on phylogeny analysis, providing fundamental data for developing targeting food safety interventions to prevent listeriosis.

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来源期刊

Foodborne pathogens and disease 医学-食品科技

CiteScore

5.30

自引率

3.60%

发文量

审稿时长

1 months

期刊介绍： Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.