Prevalence and Characteristics of Salmonella from Tibetan Pigs in Tibet, China.

IF 1.9 2区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY

Foodborne pathogens and disease Pub Date : 2024-09-01 Epub Date: 2024-07-12 DOI:10.1089/fpd.2024.0069

Gang Wang, Xilong Kang, Shuangjuan Wang, Chuang Meng, Dan Gu, Li Song, Xinan Jiao, Zhiming Pan

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Abstract

This study aimed to understand the epidemiological characteristics of Salmonella in Tibetan pigs. We isolated, identified, and examined via antimicrobial susceptibility testing on Salmonella from Tibetan pigs breeder farms and slaughterhouses in Tibet, China. A genetic evolutionary tree was constructed on the basis of whole genome sequencing (WGS). A total of 81 Salmonella isolates were isolated from 987 samples. The main serovars were Salmonella Typhimurium and Salmonella London in Tibetan pigs. The isolated Salmonella Typhimurium isolates subjected to antimicrobial susceptibility testing showed varying degrees of resistance to β-lactams, aminoglycosides, fluoroquinolones, sulfonamides, tetracyclines, and amphenicols. WGS analysis was performed on 20 Salmonella Typhimurium isolates in Tibet (n = 10), Jiangsu (n = 10), and 205 genome sequences downloaded from the Enterobase database to reveal their epidemiological and genetic characteristics. They were divided into two clusters based on core genome single-nucleotide polymorphisms: Cluster A with 112 isolates from Tibet and other regions in China and Cluster B with 113 isolates from Jiangsu and other regions. The isolates in Cluster A were further divided into two subclusters: A-1 with 40 isolates including Tibet and A-2 with 72 isolates from other regions. Virulence factors analysis revealed that all isolates from Tibet carried adeG, but this observation was not as common in Salmonella isolates from Jiangsu and other regions of China. Antibiotic resistance genes (ARGs) analysis showed that all isolates from Tibet carried bla_TEM-55 and rmtB, which were absent in Salmonella isolates from Jiangsu and other regions of China. Genetic characteristic analysis and biofilm determination indicated that the biofilm formation capabilities of the isolates from Tibet were stronger than those of the isolates from Jiangsu and other regions of China. Our research revealed the epidemic patterns and genomic characteristics of Salmonella in Tibetan pigs and provided theoretical guidance for the prevention and control of local salmonellosis.

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中国西藏藏猪沙门氏菌的流行与特征

本研究旨在了解藏猪沙门氏菌的流行病学特征。我们从中国西藏的藏猪种猪场和屠宰场分离、鉴定了沙门氏菌，并通过抗菌药物敏感性试验进行了检测。在全基因组测序（WGS）的基础上构建了基因进化树。从 987 份样本中共分离出 81 株沙门氏菌。藏猪的主要血清型为鼠伤寒沙门氏菌和伦敦沙门氏菌。经抗菌药物敏感性测试，分离出的鼠伤寒沙门氏菌对β-内酰胺类、氨基糖苷类、氟喹诺酮类、磺胺类、四环素类和安芬诺酯类具有不同程度的耐药性。对西藏（10 株）、江苏（10 株）的 20 株秋伤寒沙门氏菌分离株以及从 Enterobase 数据库下载的 205 株基因组序列进行了 WGS 分析，以揭示其流行病学和遗传学特征。根据核心基因组单核苷酸多态性将其分为两个群：A群有来自西藏和中国其他地区的112个分离株，B群有来自江苏和其他地区的113个分离株。A 群中的分离株又分为两个亚群：A-1 有 40 个分离株，包括西藏；A-2 有 72 个分离株，来自其他地区。病毒因子分析表明，西藏的所有分离株都携带 adeG，但这一现象在江苏和中国其他地区的沙门氏菌分离株中并不常见。抗生素耐药基因（ARGs）分析表明，西藏分离的所有沙门氏菌均携带 blaTEM-55 和 rmtB，而江苏和中国其他地区分离的沙门氏菌则不携带这两种基因。遗传特性分析和生物膜测定表明，西藏分离株的生物膜形成能力强于江苏和中国其他地区的分离株。我们的研究揭示了西藏猪沙门氏菌的流行规律和基因组特征，为当地沙门氏菌病的防控提供了理论指导。

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来源期刊

Foodborne pathogens and disease 医学-食品科技

CiteScore

5.30

自引率

3.60%

发文量

审稿时长

1 months

期刊介绍： Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.